ZA200103669B - Hypoxia-inducible factor 1a HIF-1α variants and methods for identifying HIF-1α modulators. - Google Patents
Hypoxia-inducible factor 1a HIF-1α variants and methods for identifying HIF-1α modulators. Download PDFInfo
- Publication number
- ZA200103669B ZA200103669B ZA200103669A ZA200103669A ZA200103669B ZA 200103669 B ZA200103669 B ZA 200103669B ZA 200103669 A ZA200103669 A ZA 200103669A ZA 200103669 A ZA200103669 A ZA 200103669A ZA 200103669 B ZA200103669 B ZA 200103669B
- Authority
- ZA
- South Africa
- Prior art keywords
- hif
- seq
- human
- domain
- variant
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 38
- 206010021143 Hypoxia Diseases 0.000 title claims description 158
- 101001046870 Homo sapiens Hypoxia-inducible factor 1-alpha Proteins 0.000 title abstract 10
- 102100022875 Hypoxia-inducible factor 1-alpha Human genes 0.000 title 2
- 230000000694 effects Effects 0.000 claims abstract description 127
- 210000004899 c-terminal region Anatomy 0.000 claims abstract description 39
- 150000001413 amino acids Chemical class 0.000 claims abstract description 30
- 150000001875 compounds Chemical class 0.000 claims abstract description 19
- 102000040945 Transcription factor Human genes 0.000 claims abstract description 18
- 108091023040 Transcription factor Proteins 0.000 claims abstract description 18
- 230000035772 mutation Effects 0.000 claims abstract description 16
- 230000030648 nucleus localization Effects 0.000 claims abstract description 15
- 210000004027 cell Anatomy 0.000 claims description 189
- 108090001146 Nuclear Receptor Coactivator 1 Proteins 0.000 claims description 119
- 102100037223 Nuclear receptor coactivator 1 Human genes 0.000 claims description 119
- 230000007954 hypoxia Effects 0.000 claims description 81
- 108700008625 Reporter Genes Proteins 0.000 claims description 66
- 102100039556 Galectin-4 Human genes 0.000 claims description 63
- 101000608765 Homo sapiens Galectin-4 Proteins 0.000 claims description 60
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims description 42
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims description 42
- 239000005090 green fluorescent protein Substances 0.000 claims description 41
- 230000004568 DNA-binding Effects 0.000 claims description 29
- 101000602930 Homo sapiens Nuclear receptor coactivator 2 Proteins 0.000 claims description 25
- 102100037226 Nuclear receptor coactivator 2 Human genes 0.000 claims description 25
- 102100032742 Histone-lysine N-methyltransferase SETD2 Human genes 0.000 claims description 23
- 101000654725 Homo sapiens Histone-lysine N-methyltransferase SETD2 Proteins 0.000 claims description 23
- 108091006108 transcriptional coactivators Proteins 0.000 claims description 13
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 239000003068 molecular probe Substances 0.000 claims description 7
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 6
- 108091027981 Response element Proteins 0.000 claims description 6
- 210000000805 cytoplasm Anatomy 0.000 claims description 6
- 230000004807 localization Effects 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 5
- 102000003951 Erythropoietin Human genes 0.000 claims description 4
- 108090000394 Erythropoietin Proteins 0.000 claims description 4
- 229940105423 erythropoietin Drugs 0.000 claims description 4
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims description 4
- 102000034356 gene-regulatory proteins Human genes 0.000 claims description 3
- 108091006104 gene-regulatory proteins Proteins 0.000 claims description 3
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 claims description 3
- 108090000331 Firefly luciferases Proteins 0.000 claims description 2
- 239000007850 fluorescent dye Substances 0.000 claims description 2
- 230000003081 coactivator Effects 0.000 abstract description 16
- 102000049131 human HIF1A Human genes 0.000 abstract 8
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 72
- 108090000623 proteins and genes Proteins 0.000 description 72
- 230000001419 dependent effect Effects 0.000 description 70
- 235000018102 proteins Nutrition 0.000 description 61
- 102000004169 proteins and genes Human genes 0.000 description 61
- 108020001507 fusion proteins Proteins 0.000 description 57
- 102000037865 fusion proteins Human genes 0.000 description 57
- 101100221606 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) COS7 gene Proteins 0.000 description 46
- 230000004913 activation Effects 0.000 description 43
- 230000001146 hypoxic effect Effects 0.000 description 42
- 230000006870 function Effects 0.000 description 38
- 229910052760 oxygen Inorganic materials 0.000 description 31
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 30
- 238000002474 experimental method Methods 0.000 description 29
- 230000033228 biological regulation Effects 0.000 description 27
- 235000001014 amino acid Nutrition 0.000 description 25
- 230000012223 nuclear import Effects 0.000 description 24
- 102100026277 Alpha-galactosidase A Human genes 0.000 description 23
- 101000718525 Homo sapiens Alpha-galactosidase A Proteins 0.000 description 23
- 238000001890 transfection Methods 0.000 description 23
- 239000013604 expression vector Substances 0.000 description 22
- 230000007959 normoxia Effects 0.000 description 22
- 210000004940 nucleus Anatomy 0.000 description 21
- 230000004927 fusion Effects 0.000 description 20
- 230000014509 gene expression Effects 0.000 description 20
- 230000001939 inductive effect Effects 0.000 description 20
- 230000002103 transcriptional effect Effects 0.000 description 20
- 230000003993 interaction Effects 0.000 description 19
- 230000004044 response Effects 0.000 description 19
- 108060001084 Luciferase Proteins 0.000 description 18
- 230000001404 mediated effect Effects 0.000 description 18
- 102000018720 Basic Helix-Loop-Helix Transcription Factors Human genes 0.000 description 17
- 108010027344 Basic Helix-Loop-Helix Transcription Factors Proteins 0.000 description 17
- 102100038885 Histone acetyltransferase p300 Human genes 0.000 description 16
- 101000882390 Homo sapiens Histone acetyltransferase p300 Proteins 0.000 description 16
- 101000978776 Mus musculus Neurogenic locus notch homolog protein 1 Proteins 0.000 description 16
- 230000010474 transient expression Effects 0.000 description 16
- 239000005089 Luciferase Substances 0.000 description 15
- 102000002131 PAS domains Human genes 0.000 description 15
- 108050009469 PAS domains Proteins 0.000 description 15
- 238000003306 harvesting Methods 0.000 description 15
- 239000013612 plasmid Substances 0.000 description 15
- ROFVEXUMMXZLPA-UHFFFAOYSA-N Bipyridyl Chemical group N1=CC=CC=C1C1=CC=CC=N1 ROFVEXUMMXZLPA-UHFFFAOYSA-N 0.000 description 14
- 238000012217 deletion Methods 0.000 description 14
- 230000037430 deletion Effects 0.000 description 14
- 230000000638 stimulation Effects 0.000 description 14
- 230000001086 cytosolic effect Effects 0.000 description 13
- 230000005937 nuclear translocation Effects 0.000 description 13
- 125000003275 alpha amino acid group Chemical group 0.000 description 12
- 230000004942 nuclear accumulation Effects 0.000 description 12
- 230000007115 recruitment Effects 0.000 description 11
- 239000012634 fragment Substances 0.000 description 10
- 230000007246 mechanism Effects 0.000 description 10
- 238000011282 treatment Methods 0.000 description 10
- 101150024767 arnT gene Proteins 0.000 description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 9
- 238000009739 binding Methods 0.000 description 9
- 239000001301 oxygen Substances 0.000 description 9
- 230000027455 binding Effects 0.000 description 8
- 239000013613 expression plasmid Substances 0.000 description 8
- 230000005714 functional activity Effects 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 230000002018 overexpression Effects 0.000 description 8
- 238000003571 reporter gene assay Methods 0.000 description 8
- 238000013518 transcription Methods 0.000 description 8
- 230000035897 transcription Effects 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000006471 dimerization reaction Methods 0.000 description 7
- 230000006698 induction Effects 0.000 description 7
- 230000003389 potentiating effect Effects 0.000 description 7
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 6
- 230000002708 enhancing effect Effects 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 230000036515 potency Effects 0.000 description 6
- 230000020874 response to hypoxia Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000701022 Cytomegalovirus Species 0.000 description 5
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
- 102000006601 Thymidine Kinase Human genes 0.000 description 5
- 108020004440 Thymidine kinase Proteins 0.000 description 5
- 230000000875 corresponding effect Effects 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 230000006641 stabilisation Effects 0.000 description 5
- 238000011105 stabilization Methods 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 4
- 102100021147 DNA mismatch repair protein Msh6 Human genes 0.000 description 4
- 102000003893 Histone acetyltransferases Human genes 0.000 description 4
- 108090000246 Histone acetyltransferases Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 102000005421 acetyltransferase Human genes 0.000 description 4
- 108020002494 acetyltransferase Proteins 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000030570 cellular localization Effects 0.000 description 4
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 4
- 230000006702 hypoxic induction Effects 0.000 description 4
- 238000003119 immunoblot Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000003446 ligand Substances 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 4
- 229930182817 methionine Natural products 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 102000005962 receptors Human genes 0.000 description 4
- 108020003175 receptors Proteins 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 102000005969 steroid hormone receptors Human genes 0.000 description 4
- 238000003146 transient transfection Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000012983 Dulbecco’s minimal essential medium Substances 0.000 description 3
- 108091006052 GFP-tagged proteins Proteins 0.000 description 3
- 108010001515 Galectin 4 Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101710128836 Large T antigen Proteins 0.000 description 3
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 3
- 108020005497 Nuclear hormone receptor Proteins 0.000 description 3
- 230000033115 angiogenesis Effects 0.000 description 3
- 231100000673 dose–response relationship Toxicity 0.000 description 3
- 230000010437 erythropoiesis Effects 0.000 description 3
- 235000013861 fat-free Nutrition 0.000 description 3
- 230000034659 glycolysis Effects 0.000 description 3
- 230000006553 hypoxic activation Effects 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000010606 normalization Methods 0.000 description 3
- 102000006255 nuclear receptors Human genes 0.000 description 3
- 108020004017 nuclear receptors Proteins 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
- 108020003113 steroid hormone receptors Proteins 0.000 description 3
- 230000004960 subcellular localization Effects 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- 230000001052 transient effect Effects 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101100534223 Caenorhabditis elegans src-1 gene Proteins 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000008300 Mutant Proteins Human genes 0.000 description 2
- 108010021466 Mutant Proteins Proteins 0.000 description 2
- 241000714474 Rous sarcoma virus Species 0.000 description 2
- 102100023132 Transcription factor Jun Human genes 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- -1 aryl hydrocarbon Chemical class 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000005014 ectopic expression Effects 0.000 description 2
- 239000000833 heterodimer Substances 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000003365 immunocytochemistry Methods 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000007423 screening assay Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
- 230000003827 upregulation Effects 0.000 description 2
- VHJLVAABSRFDPM-UHFFFAOYSA-N 1,4-dithiothreitol Chemical compound SCC(O)C(O)CS VHJLVAABSRFDPM-UHFFFAOYSA-N 0.000 description 1
- PFFIDZXUXFLSSR-UHFFFAOYSA-N 1-methyl-N-[2-(4-methylpentan-2-yl)-3-thienyl]-3-(trifluoromethyl)pyrazole-4-carboxamide Chemical compound S1C=CC(NC(=O)C=2C(=NN(C)C=2)C(F)(F)F)=C1C(C)CC(C)C PFFIDZXUXFLSSR-UHFFFAOYSA-N 0.000 description 1
- HGUFODBRKLSHSI-UHFFFAOYSA-N 2,3,7,8-tetrachloro-dibenzo-p-dioxin Chemical compound O1C2=CC(Cl)=C(Cl)C=C2OC2=C1C=C(Cl)C(Cl)=C2 HGUFODBRKLSHSI-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 101150052384 50 gene Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108010040163 CREB-Binding Protein Proteins 0.000 description 1
- 102100021975 CREB-binding protein Human genes 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 108010045171 Cyclic AMP Response Element-Binding Protein Proteins 0.000 description 1
- 102000005636 Cyclic AMP Response Element-Binding Protein Human genes 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108091035710 E-box Proteins 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241001125671 Eretmochelys imbricata Species 0.000 description 1
- 102100037362 Fibronectin Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 102000003676 Glucocorticoid Receptors Human genes 0.000 description 1
- 108090000079 Glucocorticoid Receptors Proteins 0.000 description 1
- 208000009889 Herpes Simplex Diseases 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101000602926 Homo sapiens Nuclear receptor coactivator 1 Proteins 0.000 description 1
- 101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- 241000254158 Lampyridae Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101100494944 Mus musculus Crebbp gene Proteins 0.000 description 1
- 101100506764 Mus musculus Hif1a gene Proteins 0.000 description 1
- 101100519838 Mus musculus Pag1 gene Proteins 0.000 description 1
- 101100202329 Mus musculus Slc6a11 gene Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000009869 Neu-Laxova syndrome Diseases 0.000 description 1
- 102000011779 Nitric Oxide Synthase Type II Human genes 0.000 description 1
- 108010076864 Nitric Oxide Synthase Type II Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 102000007999 Nuclear Proteins Human genes 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 102000002488 Nucleoplasmin Human genes 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 1
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004245 Proteasome Endopeptidase Complex Human genes 0.000 description 1
- 108090000708 Proteasome Endopeptidase Complex Proteins 0.000 description 1
- 102100027584 Protein c-Fos Human genes 0.000 description 1
- 229940123573 Protein synthesis inhibitor Drugs 0.000 description 1
- 108010071563 Proto-Oncogene Proteins c-fos Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 101100289792 Squirrel monkey polyomavirus large T gene Proteins 0.000 description 1
- 108010085012 Steroid Receptors Proteins 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 108010018242 Transcription Factor AP-1 Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 101000968944 Xenopus laevis Nucleoplasmin Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 108091006374 cAMP receptor proteins Proteins 0.000 description 1
- 230000008209 cardiovascular development Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000006552 constitutive activation Effects 0.000 description 1
- 230000037011 constitutive activity Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 150000002019 disulfides Chemical class 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000001378 electrochemiluminescence detection Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 238000010230 functional analysis Methods 0.000 description 1
- 238000002825 functional assay Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 102000053772 human NCOA1 Human genes 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000010189 intracellular transport Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 239000007758 minimum essential medium Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000030147 nuclear export Effects 0.000 description 1
- 230000025308 nuclear transport Effects 0.000 description 1
- 108060005597 nucleoplasmin Proteins 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 108091033319 polynucleotide Chemical group 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Chemical group 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 239000000007 protein synthesis inhibitor Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000037425 regulation of transcription Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 108091006091 regulatory enzymes Proteins 0.000 description 1
- 230000000754 repressing effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 102220285270 rs1553408350 Human genes 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4703—Inhibitors; Suppressors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Amplifiers (AREA)
- Semiconductor Lasers (AREA)
- Radar Systems Or Details Thereof (AREA)
- Testing, Inspecting, Measuring Of Stereoscopic Televisions And Televisions (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9803891A SE9803891D0 (sv) | 1998-11-13 | 1998-11-13 | Protein Variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200103669B true ZA200103669B (en) | 2002-05-07 |
Family
ID=20413284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200103669A ZA200103669B (en) | 1998-11-13 | 2001-05-07 | Hypoxia-inducible factor 1a HIF-1α variants and methods for identifying HIF-1α modulators. |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP1141005B1 (de) |
| JP (1) | JP2002530063A (de) |
| KR (1) | KR100648761B1 (de) |
| CN (1) | CN1330666C (de) |
| AT (1) | ATE374210T1 (de) |
| AU (1) | AU761594B2 (de) |
| BR (1) | BR9915263A (de) |
| CA (1) | CA2350830A1 (de) |
| DE (1) | DE69937199D1 (de) |
| HK (1) | HK1043372B (de) |
| NZ (1) | NZ511450A (de) |
| SE (1) | SE9803891D0 (de) |
| TR (1) | TR200101345T2 (de) |
| WO (1) | WO2000029437A1 (de) |
| ZA (1) | ZA200103669B (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9911047D0 (en) * | 1999-05-12 | 1999-07-14 | Isis Innovation | Assay method and means |
| ATE383877T1 (de) * | 1999-06-04 | 2008-02-15 | Dana Farber Cancer Inst Inc | Identifikation von stoffen, die transkriptionelle antworten auf hypoxia modifizieren |
| AU2001265039B2 (en) | 2000-05-26 | 2007-03-29 | Board Of Trustees Of The University Of Illinois | Reagents and methods for identifying and modulating expression of genes regulated by retinoids |
| WO2002012326A2 (en) * | 2000-08-07 | 2002-02-14 | Angiogenetics Sweden Ab | Mechanism of conditional regulation of the hypoxia-inducible factor-1 by the von hippel-lindau tumor suppressor protein |
| US20030045830A1 (en) * | 2001-04-17 | 2003-03-06 | De Bizemont Therese | Gene therapy with chimeric oligonucleotides delivered by a method comprising a step of iontophoresis |
| EP1842856A3 (de) * | 2001-05-23 | 2007-12-12 | Angiogene Inc. | Hypoxieerzeugende Faktoren und ihre Verwendung zur Herbeiführung von Angiogenese und Verbesserung der Muskelfunktionen |
| CA2447958A1 (en) | 2001-05-23 | 2002-11-28 | Angiogene Inc. | Hypoxia inducible factors and uses thereof for inducing angiogenesis and improving muscular functions |
| AU2003201798A1 (en) * | 2002-01-11 | 2003-09-02 | Angio Genetics Sweden Ab | Hif-1 alpha proteins mutated in the c-terminal transactivation domain |
| CN100366747C (zh) * | 2005-03-08 | 2008-02-06 | 上海第二医科大学附属仁济医院 | 低氧诱导因子小干扰rna质粒 |
| CN100418574C (zh) * | 2005-10-26 | 2008-09-17 | 王成球 | 一类封闭人类HIF-1α基因和调控网络中下游相关基因的染色质肽 |
| CN102127161B (zh) * | 2010-12-16 | 2013-03-27 | 何明忠 | 抗肿瘤多肽及其用途 |
| CN106366173A (zh) * | 2015-07-24 | 2017-02-01 | 北京京爱康科技有限公司 | 一种通过结合低氧反应元件控制缺氧反应基因表达的染色质肽 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5882914A (en) * | 1995-06-06 | 1999-03-16 | The Johns Hopkins University School Of Medicine | Nucleic acids encoding the hypoxia inducible factor-1 |
-
1998
- 1998-11-13 SE SE9803891A patent/SE9803891D0/xx unknown
-
1999
- 1999-11-11 NZ NZ511450A patent/NZ511450A/en unknown
- 1999-11-11 JP JP2000582422A patent/JP2002530063A/ja active Pending
- 1999-11-11 AT AT99958594T patent/ATE374210T1/de not_active IP Right Cessation
- 1999-11-11 EP EP99958594A patent/EP1141005B1/de not_active Expired - Lifetime
- 1999-11-11 WO PCT/SE1999/002053 patent/WO2000029437A1/en active IP Right Grant
- 1999-11-11 TR TR2001/01345T patent/TR200101345T2/xx unknown
- 1999-11-11 BR BR9915263-0A patent/BR9915263A/pt not_active Application Discontinuation
- 1999-11-11 AU AU15931/00A patent/AU761594B2/en not_active Ceased
- 1999-11-11 KR KR1020017005928A patent/KR100648761B1/ko not_active IP Right Cessation
- 1999-11-11 CN CNB99814388XA patent/CN1330666C/zh not_active Expired - Fee Related
- 1999-11-11 CA CA002350830A patent/CA2350830A1/en not_active Abandoned
- 1999-11-11 DE DE69937199T patent/DE69937199D1/de not_active Expired - Lifetime
-
2001
- 2001-05-07 ZA ZA200103669A patent/ZA200103669B/en unknown
-
2002
- 2002-07-09 HK HK02105080.8A patent/HK1043372B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1141005B1 (de) | 2007-09-26 |
| ATE374210T1 (de) | 2007-10-15 |
| JP2002530063A (ja) | 2002-09-17 |
| KR20010080411A (ko) | 2001-08-22 |
| WO2000029437A1 (en) | 2000-05-25 |
| CA2350830A1 (en) | 2000-05-25 |
| HK1043372B (zh) | 2008-05-09 |
| NZ511450A (en) | 2003-08-29 |
| SE9803891D0 (sv) | 1998-11-13 |
| BR9915263A (pt) | 2001-10-30 |
| TR200101345T2 (tr) | 2001-10-22 |
| AU1593100A (en) | 2000-06-05 |
| CN1330663A (zh) | 2002-01-09 |
| EP1141005A1 (de) | 2001-10-10 |
| CN1330666C (zh) | 2007-08-08 |
| DE69937199D1 (de) | 2007-11-08 |
| HK1043372A1 (en) | 2002-09-13 |
| AU761594B2 (en) | 2003-06-05 |
| KR100648761B1 (ko) | 2006-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Morris et al. | Myosin VI binds to and localises with Dab2, potentially linking receptor‐mediated endocytosis and the actin cytoskeleton | |
| Daniely et al. | Formation of a complex between nucleolin and replication protein A after cell stress prevents initiation of DNA replication | |
| Bex et al. | Differential transcriptional activation by human T-cell leukemia virus type 1 Tax mutants is mediated by distinct interactions with CREB binding protein and p300 | |
| Zhang et al. | Disruption of chaperone-mediated autophagy-dependent degradation of MEF2A by oxidative stress-induced lysosome destabilization | |
| US5523227A (en) | DNA encoding calcium-signal modulating cyclophilin ligand | |
| Brunet et al. | RhoGDI‐3 regulates RhoG and targets this protein to the Golgi complex through its unique N‐terminal domain | |
| Lischka et al. | A nonconventional nuclear localization signal within the UL84 protein of human cytomegalovirus mediates nuclear import via the importin α/β pathway | |
| EP1141005B1 (de) | Verfahren zur identifikation von modulatoren von hif-1-alpha | |
| US6436654B1 (en) | Methods for identifying compounds that modulate HIF-1α | |
| Weber-Boyvat et al. | ORP/Osh mediate cross-talk between ER-plasma membrane contact site components and plasma membrane SNAREs | |
| Piluso et al. | Host cell factor-1 interacts with and antagonizes transactivation by the cell cycle regulatory factor Miz-1 | |
| Cordes et al. | Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae | |
| US20070231835A1 (en) | Proteomic Screening for Redox State Dependent Protein-Protein Interactions | |
| Chan et al. | DNA elements of the type 1 adenylyl cyclase gene locus enhance reporter gene expression in neurons and pinealocytes | |
| US7081343B2 (en) | Methods for identifying modulators of NF-KB activity | |
| MXPA01004787A (en) | HYPOXIA-INDUCIBLE FACTOR 1&agr;HIF-1&agr;VARIANTS AND METHODS FOR IDENTIFYING HIF-1&agr;MODULATORS | |
| Chien et al. | The A20-binding protein ABIN-2 exerts unexpected function in mediating transcriptional coactivation | |
| EP1637540A1 (de) | Hyperaktive Stat-Moleküle und deren Verwendung zum Testen von Genaktivierung | |
| Herzig Jr | NRF-1 regulation of respiratory function: Involvement of phosphorylation and protein-protein interactions | |
| McNamar | Identifying Components of the Mammalian RNA Polymerase I Apparatus Required for rDNA Transcription | |
| Newman | The small GTPase ARL2 is a novel regulator of mitochondrial fusion | |
| Feng | Study of Short Forms of P/Q-Type Voltage-Gated Calcium Channels | |
| Reilein | Signals regulating organelle transport in melanophores | |
| Zhang | Regulation of the activity of Erythroid Krüppel-like Factor (EKLF) by histone acetyltransferases | |
| Jeffries | THE ROLE OF NUCLEAR LOCALIZATION AND N ic-PROTEIN COMPLEX FORMATION IN NOTCH INDUCED NEOPLASIA |