WO2024117733A1 - Method for cultivating cordyceps militaris with high content of collagen and cordycepin, and method for extracting high-purity collagen and cordycepin - Google Patents

Method for cultivating cordyceps militaris with high content of collagen and cordycepin, and method for extracting high-purity collagen and cordycepin Download PDF

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WO2024117733A1
WO2024117733A1 PCT/KR2023/019337 KR2023019337W WO2024117733A1 WO 2024117733 A1 WO2024117733 A1 WO 2024117733A1 KR 2023019337 W KR2023019337 W KR 2023019337W WO 2024117733 A1 WO2024117733 A1 WO 2024117733A1
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collagen
cordyceps sinensis
cordycepin
high content
weight
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PCT/KR2023/019337
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French (fr)
Korean (ko)
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김세은
김배용
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주식회사 바이오로지텍
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Publication of WO2024117733A1 publication Critical patent/WO2024117733A1/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/40—Cultivation of spawn
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/50—Inoculation of spawn
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/60—Cultivation rooms; Equipment therefor
    • A01G18/69—Arrangements for managing the environment, e.g. sprinklers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
    • A23L29/281—Proteins, e.g. gelatin or collagen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L35/00—Food or foodstuffs not provided for in groups A23L5/00 – A23L33/00; Preparation or treatment thereof

Definitions

  • the present invention relates to a method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin and a method of extracting high purity collagen and cordycepin from Cordyceps sinensis.
  • Cordyceps sinensis refers to all types of mushrooms that parasitize insects. About 300 to 400 species have been discovered around the world, and about 80 species have been discovered in Korea. Representative insects that Cordyceps sinensis inhabits include bees, ants, dragonflies, butterflies, cicadas, stink bugs, beetles, flies, spiders, and crickets. These insects are invaded in the form of eggs, larvae, pupae, and adults.
  • Mushrooms formed by insects infected with Cordyceps sinensis have a variety of beautiful colors, including red, yellow, purple, green, black, white, orange, and olive.
  • Cordyceps sinensis is very difficult to find because it is very small and has a difficult habitat environment. In most cases, they are found in places where natural conditions are maintained, with clean air, high humidity, and adequate shade from trees.
  • Cordyceps sinensis is a mushroom named after the fact that it lives in the bodies of insects in the winter and appears like grass in the summer.
  • Cordyceps sinensis is an insect-parasitic medicinal mushroom that occurs when Cordyceps sinensis, a type of fungus, enters the body of a living insect. Insects infected with Cordyceps sinensis are characterized by not rotting and maintaining their mummy-like shape even after death until mushrooms emerge.
  • the conventional cultivation method for Cordyceps sinensis involves inoculating spawn into a medium containing rapeseed mixture and green algae and allowing it to germinate in dark culture and grow in light culture.
  • technology has been developed to enable the cultivation of Cordyceps sinensis malataris using culture methods such as dark culture and light culture rather than media, there were limitations in improving the various functionality of Cordyceps sinensis with these conventional culture methods alone. will be.
  • Cordycepin is known as a bioactive substance that embodies the efficacy of Cordyceps sinensis. It is a natural compound of the 3-deoxyadenosine family and has been shown in clinical trials to have antibacterial, antitumor and anticancer effects. Therefore, various studies have been conducted in depth to increase the cordycepin content of Cordyceps sinensis.
  • collagen is a protein that accounts for 1/3 of all proteins in mammals and is the most abundant protein in the human body.
  • the molecular structure of collagen consists of three chains of about 1,000 amino acids, and the polypeptide of collagen consists of a repeating sequence in which glycine is repeated every three times.
  • the main amino acids include proline and hydroxyproline.
  • Gly-Pro-Hyp composed of three amino acids, is known to be a representative structural peptide of collagen.
  • collagen is characterized by a high content of hydroxyproline, which does not exist in other proteins.
  • the object of the present invention is to cultivate Cordyceps sinensis to contain a high content of collagen and cordycepin by allowing Cordyceps to absorb the collagen consumed by the host when cultivating Cordyceps sinensis by inoculating a host with a seed fungus, and to cultivate the plant to contain a high content of collagen and cordycepin.
  • the purpose is to provide a method for extracting with high purity.
  • the present invention relates to a method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin, specifically a first step of aerobic fermentation and fermentation of the mixture to form food for the host; A second process of collecting eggs using the food as a breeding medium and growing them into larvae; A third process of rapidly freezing the larvae and completing them as hosts; A fourth step of preparing mycelium by adding seed to a culture prepared by diluting collagen powder and culturing it; And a fifth step of cultivating Cordyceps sinensis by inoculating the prepared mycelium into a host and maintaining humidity.
  • the present invention relates to a method for extracting high purity collagen and cordycepin from Cordyceps sinensis, comprising the steps of mixing Cordyceps sinensis, water, and carbohydrate decomposing enzyme and then performing primary extraction; A process of cooling the primary extract, adding fibrous enzyme and proteolytic enzyme, and performing secondary extraction; And a process of filtration and concentration through a deactivation process.
  • the method of cultivating Cordyceps sinensis containing collagen uses plants containing collagen, a desired specific ingredient, as food for the host of Cordyceps sinensis, so that the specific ingredient is naturally ingested and introduced into the host's body by the host's feeding activity,
  • Cordyceps sinensis absorbs specific ingredients consumed by the host, which has the effect of allowing Cordyceps sinensis to naturally absorb a large amount of collagen.
  • specific ingredients are absorbed by Cordyceps sinensis. This has the effect of allowing Cordyceps sinensis to more easily supply the collagen component to Cordyceps sinensis through the host, allowing Cordyceps sinensis to contain a large amount of collagen.
  • Cordyceps sinensis containing collagen can be cultivated simply by feeding the collagen-containing plant as food to the host of Cordyceps sinensis, so the cultivation method is extremely easy, providing convenience during cultivation and reducing the labor of workers. It has a number of effects, including being able to significantly reduce fatigue.
  • a fifth step of cultivating Cordyceps sinensis by inoculating the prepared mycelium into the host and maintaining humidity.
  • the cultivation method according to the present invention is characterized by rapidly freezing hosts grown on food containing essential substances containing the characteristic components desired to be contained in Cordyceps sinensis and cultivating them by inoculating them with spawn.
  • the cultivation method according to the present invention is characterized by containing a high content of cordycepin by periodically applying cryogenic shock below 3°C, and specifically, the brown rice medium mixture containing Cordyceps sinensis is grown in the first culture medium at 3°C or below. It includes a culturing step in which a low-temperature shock cycle of sequential culturing at a temperature and a second temperature of 20° C. or higher is repeatedly performed.
  • the “cryogenic shock” means including a means of applying a temperature of 3°C or lower to the Cordyceps fungi when cultivating Cordyceps sinensis and a means of applying a temperature difference of 17°C or higher.
  • a low-temperature shock cycle in which Cordyceps sinensis is sequentially cultured at the first temperature and the second temperature may be used.
  • the low-temperature shock cycle includes t1) reducing the temperature of the brown rice medium mixture to the first temperature, t2) increasing the temperature of the reduced brown rice medium mixture to the second temperature, and t3) raising the temperature of the brown rice medium mixture. and maintaining the mixture at the second temperature.
  • the first temperature is preferably 3°C or lower, preferably 1°C or lower, and the lower limit may be -2°C.
  • a cryogenic shock is applied to the Cordyceps sinensis with a high probability, so that the immunity and environmental adaptability of the bacteria are significantly increased compared to the case where the cryogenic shock is not applied. It is possible to cultivate brown rice Cordyceps sinensis with improved cordycepin content.
  • the second temperature may be 20°C or higher, specifically 20 to 30°C.
  • the second temperature is set to less than 20°C, even if a cryogenic state is induced at the first temperature, a temperature deviation of 17°C or more is not applied, so the cryogenic shock can be alleviated, and an increase in the cordycepin content described above can be expected. does not exist.
  • the step t1) may be preferably performed for 1 to 3 days, specifically 1.5 to 2.5 days. If this is satisfied, it is possible to quickly and smoothly apply a cryogenic shock to the Cordyceps sinensis bacteria and prevent a decrease in the growth or inactivation of the Cordyceps sinensis bacteria due to a prolonged cryogenic shock being applied.
  • Step t2) may be preferably performed for 3 to 7 days, specifically 4 to 6 days, and step t3) may be preferably performed for 5 to 10 days, specifically 6 to 8 days. If this is satisfied, it is possible to effectively apply cryogenic shock due to temperature deviation and prevent growth deterioration or inactivation of Cordyceps sinensis due to intermittent provision of an appropriate growth temperature.
  • the step t1) may include a process of reducing the temperature to the first temperature and a process of maintaining the first temperature. It may be preferable that the process of reducing the temperature to the first temperature is a process of rapid temperature reduction. Specifically, the temperature reduction rate in the process may be 1 to 10°C/min. However, this is only described as a preferred example, and the present invention is not necessarily limited thereto.
  • the step t2) may include a process of increasing the temperature to the second temperature and maintaining the second temperature. It may be preferable that the process of raising the temperature to the second temperature is a process of rapid temperature increase. Specifically, the temperature increase rate in the process may be 0.01 to 0.5°C/min. However, this is only described as a preferred example, and the present invention is not necessarily limited thereto.
  • the step t3) is a step of continuously maintaining the second temperature of the step t2) for a certain period of time. If this step t3) is further performed, it is possible to prevent growth deterioration or inactivation of Cordyceps sinensis due to intermittent provision of an appropriate growth temperature even when cryogenic shock is applied.
  • the low-temperature shock cycle is preferably performed two or more times, preferably three or more times, and specifically 2 to 4 times. If this is satisfied, by periodically applying cryogenic shock to the Cordyceps sinensis, the immunity and environmental adaptability of the bacteria are significantly improved compared to the case where it is performed only once, making it possible to cultivate brown rice Cordyceps sinensis with a further increased cordycepin content.
  • the culturing step it may be desirable for the culturing to be performed at a relative humidity of 50 to 80%. If this is satisfied, it is possible to prevent the problem of the growth of Cordyceps sinensis being inhibited as the above-described low-temperature shock cycle is repeatedly performed, and to provide a smooth growth environment.
  • the step of preparing the brown rice medium mixture may be further included.
  • the step of preparing the brown rice medium mixture may include the step of preparing the brown rice medium, the step of preparing the seed mixture medium, and the step of inoculating the seed mixture medium into the brown rice medium to prepare the brown rice medium mixture.
  • the mixture can be aerobically fermented and fermented to form food for the host.
  • the mixture includes 20-30% by weight of plants, 40-50% by weight of oak sawdust, 1-3% by weight of useful microorganisms, 4-6% by weight of molasses, 5-10% by weight of soybean meal, 5-10% by weight of wheat bran, and 4-4% of water. It is preferably prepared by mixing 12% by weight.
  • the first aerobic fermentation lasts for 5 weeks, and then the second and third fermentations are mixed by supplying moisture at four-week intervals to allow additional aerobic fermentation to occur, thereby minimizing the generation of fermentation heat during rearing and forming it into food for the host. desirable.
  • the above plants include Malvaceae and Malvaceae, Malvaceae seeds, Marshmallow, Rose of Sharon from the Rose of Sharon family, Rose of Sharon from the Rose of Sharon family, Root bark of the Rose of Sharon, Mulberry mulberry, Hundreds of grass, Eucalyptus officinalis, Euphyllaceae, Arrowaceae, Argentine vine, Arrowroot tree, Perpendicularis tree, Siberian chinensis, Geumhwagyu, Sinseoncho, Okra, Blochia, Cotton, Buyongcha, Elm, Radix Bark, Bodhi Tree, Althea, Tabacco, Maize, Alfalfa, Rice, Potato, Soybean ), tomatoes, wheat, barley, canola, carrots, and cotton, etc., are crushed and extracted. However, if it is a plant containing collagen, this is not recommended. It is not limited.
  • the hosts may include bees, ants, dragonflies, butterflies, cicadas, stink bugs, beetles, flies, spiders, and crickets.
  • white-spotted flower beetles also known as flower beetles, as the hosts.
  • snail mucus extract is used instead of the plant containing the collagen, or the plant is mixed with the snail mucus extract.
  • the mixture containing the snail mucus extract is preferably contained at 20-30% by weight, the same as the content of the collagen-containing plant.
  • the snail mucus extract is a natural product and is a cell secretion containing glycoprotein and mucopolysaccharide, and contains mucin, allantoin, glycolic acid, collagen, and elastin ( elastin) is included.
  • the method of obtaining the snail mucus extract is to separate the mucus from the snail, add a sufficient amount of extraction solvent to the mucus at room temperature or 20-40°C, and shake to allow the reaction to occur. All known extraction methods can be used, but high-temperature hot water extraction is not preferable because it can cause denaturation or functional deterioration of proteins.
  • the extraction solvent includes aqueous solvents (e.g., water, acidic or basic aqueous solvents, etc.), alcohols (e.g., methanol, anhydrous ethanol, lower alcohols such as ethanol, or propylene glycol, 1,3-butyl).
  • aqueous solvents e.g., water, acidic or basic aqueous solvents, etc.
  • alcohols e.g., methanol, anhydrous ethanol, lower alcohols such as ethanol, or propylene glycol, 1,3-butyl.
  • Polyhydric alcohols such as ren glycol, etc.
  • ketones such as acetone, etc.
  • extraction solvent is usually carried out in the range of pH 2 to pH 13, with the range of pH 4 to pH 10 being preferred, and the range of pH 4 to pH 7.5 being particularly preferred.
  • Snail mucus extract can be used in the form of a liquid extracted from snail mucus, granules prepared by drying it, powder, or a solution diluted in purified water.
  • the food prepared in the first process can be used as a breeding medium to collect eggs and then grow into larvae.
  • the second process may consist of an egg picking stage, a host breeding stage, and a host completion stage.
  • the food prepared through the above first process was used as a breeding medium and the moisture was adjusted to 65% water content. Then, 10 kg was weighed and spread out in a 36L box. The temperature of the breeding room was set to 28°C for smooth egg picking. It is desirable to set It is preferable to provide an egg picking step in which 60 adult hosts are introduced into the breeding medium for a week and 300 to 400 eggs are received from the adults.
  • the larvae grown in the second process can be rapidly frozen to complete the host.
  • a host completion step in which the fully grown larvae obtained through the host breeding step of the second process are rapidly frozen to complete the host.
  • mycelium can be prepared by adding seed to a culture made by diluting collagen powder and culturing it into mycelium.
  • the seed is added to a culture made by diluting the collagen extract and cultured into mycelium.
  • the present invention includes a method of feeding plants containing collagen as food to the host and a culture in which collagen is diluted to the seed.
  • the collagen component can be provided twice as Cordyceps sinensis.
  • Cordyceps militaris As the starter, but it is not limited thereto.
  • the host quenched in the third process is inoculated with the mycelium obtained in the fourth process, and then Cordyceps sinensis can be cultivated by maintaining humidity.
  • the host rapidly frozen in the host completion step is inoculated with the mycelium obtained in the mycelium preparation step, divided into 100g each container, and provided with an inoculation step of supplying moisture and irradiating light at the same time to allow the spawn to grow at 20°C.
  • the seed inoculated into the host begins to grow.
  • the temperature in the cultivation room is adjusted to 18°C, and moisture is administered through a sprayer until the growth period of 60-65 days has passed. Therefore, it is desirable to provide a Cordyceps sinensis cultivation step to cultivate Cordyceps sinensis while maintaining humidity at 65%.
  • a functional enhancer when the seed starts to grow, a functional enhancer can be added so that the cultivated Cordyceps sinensis contains a high content of collagen and cordycepin.
  • the present invention relates to a method for extracting collagen and cordycepin from Cordyceps sinensis cultivated through the above method,
  • a process of cooling the primary extract, adding fibrous enzyme and proteolytic enzyme, and performing secondary extraction A process of cooling the primary extract, adding fibrous enzyme and proteolytic enzyme, and performing secondary extraction;
  • It is characterized by including a process of filtration and concentration through a deactivation process.
  • the extraction method is characterized by using a low-temperature and high-pressure enzyme treatment method.
  • the extraction method may use water (distilled water), ethanol, etc., but it is more preferable to use water.
  • cordycepin content of Cordyceps sinensis grown according to the method of the present invention was analyzed by the following method.
  • cordycepin content was higher at 500 to 580 ppm as the number of cold shock cycles was increased by three times compared to Cordyceps sinensis cultivated by the conventional method.
  • the collagen content of Cordyceps sinensis grown according to the method of the present invention was analyzed by the following method.
  • Example 2 The same analysis sample as in Example 1 was used, and 4 mL of 3% sulfosalicylic acid was mixed with 1 mL of the analysis sample and placed in an ultrasonic disperser for 5 minutes. Mix 2 mL of acidic ninhydrin solution (ninhydrin 2.5 g/glacial acetic acid [glacial acetic acid] 6:phosphoric acid 3:distilled water 1 [V:V] 100 mL) and 2 mL of glacial acetic acid in 2 mL of the above mixture and leave for 1 hour at above 95 ⁇ 5°C. The reaction was terminated below 4°C, 4 mL of toluene was mixed, and the absorbance was measured at 520 nm. Using L-proline as a standard solution, the proline content in the sample was measured.
  • L-proline as a standard solution
  • Cordyceps sinensis sufficiently supplied in a collagen medium with the collagen feed and collagen starter through the cultivation method according to the present invention is rich in collagen components and can be a functional food with a high unit of cordycepin.
  • An extract was obtained by extracting Cordyceps sinensis grown according to the method of the present invention through a low-temperature and high-pressure enzyme treatment method.
  • the collagen and cordycepin contents of the obtained extract were measured using high-performance liquid chromatography (HPLC), and the results are shown in Table 1 below.
  • the method of cultivating Cordyceps sinensis according to the present invention can have the effect of allowing Cordyceps sinensis to contain a large amount of collagen and to have the efficacy of collagen in addition to the natural efficacy of Cordyceps sinensis.

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Abstract

The present invention relates to a method for cultivating Cordyceps militaris with a high content of collagen and cordycepin, and a method for extracting high-purity collagen and cordycepin from the Cordyceps militaris, and more specifically, to a method for cultivating Cordyceps militaris with a high content of collagen and cordycepin by supplying a sufficient amount of collagen from a collagen medium through collagen feed and collagen spawn, and a method for extracting the collagen and cordycepin in high purity.

Description

고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법 및 고순도의 콜라겐 및 코디세핀 추출 방법Method for cultivating Cordyceps sinensis containing high content of collagen and cordycepin and method for extracting high purity collagen and cordycepin
본 발명은 콜라겐 및 코디세핀이 고함량으로 함유된 동충하초의 재배방법 및 상기 동충하초로부터 고순도의 콜라겐 및 코디세핀을 추출하는 방법에 관한 것이다.The present invention relates to a method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin and a method of extracting high purity collagen and cordycepin from Cordyceps sinensis.
동충하초란 넓은 의미에서는 곤충에 기생하는 모든 종류의 버섯을 말하며, 전 세계에 300~400여 종 정도가 발견되었고, 우리나라에서는 80여 종이 발견되었다. 동충하초가 기생하는 대표적인 곤충은 벌, 개미, 잠자리, 나비, 매미, 노린재, 딱정벌레, 파리, 거미, 귀뚜라미 등이다. 이들 곤충은 알, 유충, 번데기, 성충 등의 상태에서 침입을 받게 된다. In a broad sense, Cordyceps sinensis refers to all types of mushrooms that parasitize insects. About 300 to 400 species have been discovered around the world, and about 80 species have been discovered in Korea. Representative insects that Cordyceps sinensis inhabits include bees, ants, dragonflies, butterflies, cicadas, stink bugs, beetles, flies, spiders, and crickets. These insects are invaded in the form of eggs, larvae, pupae, and adults.
동충하초에 감염된 곤충에 생긴 버섯의 색깔은 홍색, 황색, 자색, 녹색, 흑색, 백색, 오렌지색, 올리브색 등 여러 가지 아름다운 빛깔을 가지고 있다. 동충하초는 크기가 매우 작고 서식환경이 까다롭기 때문에 발견하기가 매우 어렵다. 대개의 경우 공기가 깨끗하고 습도가 높으며 적당하게 나무 그늘이 진, 자연 상태가 유지된 장소에서 많이 발견된다. Mushrooms formed by insects infected with Cordyceps sinensis have a variety of beautiful colors, including red, yellow, purple, green, black, white, orange, and olive. Cordyceps sinensis is very difficult to find because it is very small and has a difficult habitat environment. In most cases, they are found in places where natural conditions are maintained, with clean air, high humidity, and adequate shade from trees.
동충하초는 겨울에는 곤충의 몸에 있다가 여름에는 풀처럼 나타난다는데서 비롯되어 이름 지어진 버섯이다. 동충하초는 곰팡이의 일종인 동충하초균이 살아있는 곤충의 몸속으로 들어가 발생하는 곤충기생성 약용버섯이다. 동충하초균에 감염된 곤충은 버섯이 나오기 전까지는 죽어도 썩지 않고 미라처럼 형태를 유지하는 것이 특징이다. Cordyceps sinensis is a mushroom named after the fact that it lives in the bodies of insects in the winter and appears like grass in the summer. Cordyceps sinensis is an insect-parasitic medicinal mushroom that occurs when Cordyceps sinensis, a type of fungus, enters the body of a living insect. Insects infected with Cordyceps sinensis are characterized by not rotting and maintaining their mummy-like shape even after death until mushrooms emerge.
동충하초를 자연적으로 구하기가 어려워서 인공적으로 재배를 하여 대량으로 제공할 수 있는 방법을 모색하고 있으며 일부 인공 재배방법들이 개발되고 있었다. 종래의 동충하초 재배방법으로는 대한민국 등록특허 제10-1347219호에 개재된 바와 같이 유채박혼합물과 갈파래과녹조류가 함유된 배지에 종균을 접종하고 암배양으로 발아 및 광배양으로 생육토록 하는 재배방법으로 동충하초를 재배할 수 있도록 한 기술이 개발되어 왔으나, 배지보다는 암배양과 광배양 등의 배양방법에 의해서 말라타리스 동충하초를 재배토록 하였으나, 이러한 종래 배양방법으로만은 동충하초의 다양한 기능성을 향상시키는데 한계가 있었던 것이다. Since it is difficult to obtain Cordyceps sinensis naturally, methods are being sought to provide it in large quantities by artificially cultivating it, and some artificial cultivation methods are being developed. The conventional cultivation method for Cordyceps sinensis, as disclosed in Republic of Korea Patent No. 10-1347219, involves inoculating spawn into a medium containing rapeseed mixture and green algae and allowing it to germinate in dark culture and grow in light culture. Although technology has been developed to enable the cultivation of Cordyceps sinensis malataris using culture methods such as dark culture and light culture rather than media, there were limitations in improving the various functionality of Cordyceps sinensis with these conventional culture methods alone. will be.
한편, 동충하초의 효능을 구현하는 생리활성 물질로 코디세핀(Cordycpin)이 알려져 있으며, 이는 3-deoxyadenosine 계통의 천연 화합물로서 항세균, 항종양 및 항암 효과가 있음이 임상실험에서 밝혀졌다. 따라서 동충하초의 코디세핀 함량을 증대시키기 위한 다각도의 연구가 심도있게 연구되어 왔다.Meanwhile, Cordycepin is known as a bioactive substance that embodies the efficacy of Cordyceps sinensis. It is a natural compound of the 3-deoxyadenosine family and has been shown in clinical trials to have antibacterial, antitumor and anticancer effects. Therefore, various studies have been conducted in depth to increase the cordycepin content of Cordyceps sinensis.
또한, 콜라겐(Collagen)은 포유동물의 전체 단백질 중 1/3을 차지하는 단백질로서, 인체에 가장 많이 존재하는 단백질이다. 콜라겐의 분자구조는 아미노산 약 1,000개가 결합된 사슬 세가닥으로 이루어져 있고 콜라겐의 폴리펩티드는 3번 마다 글리신이 반복되는 반복서열로 구성되어 있으며, 글리신 외에 주요 아미노산으로는 프롤린, 히드록시프롤린이 있으며, 상기 세 가지 아미노산들로 이루어진 Gly-Pro-Hyp가 콜라겐을 대표하는 구성 펩티드로 알려져 있다. 특히 콜라겐에는 다른 단백질에는 존재하지 않는 히드록시프롤린의 함량이 높은 것이 특징이다.Additionally, collagen is a protein that accounts for 1/3 of all proteins in mammals and is the most abundant protein in the human body. The molecular structure of collagen consists of three chains of about 1,000 amino acids, and the polypeptide of collagen consists of a repeating sequence in which glycine is repeated every three times. In addition to glycine, the main amino acids include proline and hydroxyproline. Gly-Pro-Hyp, composed of three amino acids, is known to be a representative structural peptide of collagen. In particular, collagen is characterized by a high content of hydroxyproline, which does not exist in other proteins.
본 발명의 목적은 숙주에 종균을 접종하여 동충하초를 재배할 시 숙주가 섭취한 콜라겐을 동충하초가 흡수하도록 하여 고함량의 콜라겐 및 코디세핀을 함유하도록 동충하초를 재배하고, 상기 고함량의 콜라겐 및 코디세핀을 고순도로 추출하는 방법을 제공하는 데에 있다. The object of the present invention is to cultivate Cordyceps sinensis to contain a high content of collagen and cordycepin by allowing Cordyceps to absorb the collagen consumed by the host when cultivating Cordyceps sinensis by inoculating a host with a seed fungus, and to cultivate the plant to contain a high content of collagen and cordycepin. The purpose is to provide a method for extracting with high purity.
상기 과제를 해결하기 위하여, 본 발명은 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법에 관한 것으로, 구체적으로 혼합물을 호기발효 및 발효하여 숙주의 먹이로 조성하는 제1공정; 상기 먹이를 사육배지로 하여 채란한 후 유충으로 성장시키는 제2공정; 상기 유충을 급냉시켜 숙주로 완성하는 제3공정; 콜라겐 분말을 희석시켜 제조한 배양물에 종균을 투입하여 배양하여 균사체를 준비하는 제4공정; 및 상기 준비된 균사체를 숙주에 접종하고 습도를 유지하여 동충하초를 재배하는 제5공정;을 포함한다.In order to solve the above problems, the present invention relates to a method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin, specifically a first step of aerobic fermentation and fermentation of the mixture to form food for the host; A second process of collecting eggs using the food as a breeding medium and growing them into larvae; A third process of rapidly freezing the larvae and completing them as hosts; A fourth step of preparing mycelium by adding seed to a culture prepared by diluting collagen powder and culturing it; And a fifth step of cultivating Cordyceps sinensis by inoculating the prepared mycelium into a host and maintaining humidity.
또한, 본 발명은 동충하초로부터 고순도의 콜라겐 및 코디세핀을 추출하는 방법에 관한 것으로, 상기 동충하초, 물 및 탄수화물분해효소를 혼합한 후 1차 추출하는 공정; 상기 1차 추출물을 냉각시킨 후 섬유질분해효소 및 단백질분해효소를 첨가한 후 2차 추출하는 공정; 및 실활 과정을 거쳐 여과 및 농축하는 공정;을 포함하는 것을 특징으로 한다.In addition, the present invention relates to a method for extracting high purity collagen and cordycepin from Cordyceps sinensis, comprising the steps of mixing Cordyceps sinensis, water, and carbohydrate decomposing enzyme and then performing primary extraction; A process of cooling the primary extract, adding fibrous enzyme and proteolytic enzyme, and performing secondary extraction; And a process of filtration and concentration through a deactivation process.
본 발명에 따른 콜라겐이 함유된 동충하초 재배방법은 원하는 특정성분인 콜라겐이 함유된 식물을 동충하초의 숙주의 먹이로 사용하여 숙주의 먹이 활동에 의해서 자연스럽게 특정성분이 숙주의 몸속으로 섭취되어 유입되도록 하고, 숙주에 종균을 접종하여 동충하초를 재배할 시 숙주가 섭취한 특정성분을 동충하초가 오롯이 흡수하게 되므로 해서 동충하초가 콜라겐을 다량으로 자연스럽게 흡수할 수 있도록 하는 효과를 갖는 것으로서, 이러한 동충하초의 재배방법에 의하면 특정성분인 콜라겐을 보다 손쉽게 숙주를 통해서 동충하초로 공급토록 하여 동충하초가 콜라겐을 다량으로 함유토록 하는 효과를 가지는 것이다. The method of cultivating Cordyceps sinensis containing collagen according to the present invention uses plants containing collagen, a desired specific ingredient, as food for the host of Cordyceps sinensis, so that the specific ingredient is naturally ingested and introduced into the host's body by the host's feeding activity, When cultivating Cordyceps sinensis by inoculating a host with spawn, Cordyceps sinensis absorbs specific ingredients consumed by the host, which has the effect of allowing Cordyceps sinensis to naturally absorb a large amount of collagen. According to this cultivation method of Cordyceps sinensis, specific ingredients are absorbed by Cordyceps sinensis. This has the effect of allowing Cordyceps sinensis to more easily supply the collagen component to Cordyceps sinensis through the host, allowing Cordyceps sinensis to contain a large amount of collagen.
이와 같이 재배된 동충하초를 사람이 섭취하게 되면 당연히 동충하초 본연의 효능과 더불어서 이러한 동충하초에 함유된 콜라겐의 효능도 동시에 갖도록 하는 효과를 가지는 것이다. When a person consumes Cordyceps sinensis cultivated in this way, it naturally has the effect of having the efficacy of the collagen contained in Cordyceps sinensis in addition to the natural efficacy of Cordyceps sinensis.
이에 의하면, 본 발명에서는 콜라겐이 함유된 식물을 동충하초의 숙주의 먹이로 주기만 하는 수고로움만으로 콜라겐을 함유한 동충하초를 재배할 수 있으므로 재배방법이 극히 용이하므로 재배 시의 편의성을 제공하면서 작업자의 수고로움과 피로함을 현저히 극감시킬 수 있는 등 다수의 효과를 가지는 것이다. According to this, in the present invention, Cordyceps sinensis containing collagen can be cultivated simply by feeding the collagen-containing plant as food to the host of Cordyceps sinensis, so the cultivation method is extremely easy, providing convenience during cultivation and reducing the labor of workers. It has a number of effects, including being able to significantly reduce fatigue.
또한, 콜라겐이 함유된 식물을 먹이로 제공하여 사육한 숙주를 콜라겐 분말을 희석시켜서 만든 배양물이 내입된 배지에 종균(=밀리타리스균)을 투입하여 배양된 균사체를 상기 식물을 먹여 사육한 숙주(현미)에 접종하도록 함을 특징으로 하며, 이로 인해 숙주가 섭취한 콜라겐성분 외에도 동충하초의 종균이 배양될 시에도 콜라겐 분말이 혼합된 배양물에 의해서 콜라겐이 섭취되어 추후 동충하초가 콜라겐 성분을 한층 더 내포하고 있도록 할 수 있는 효과를 가지는 것이다. In addition, hosts raised by providing collagen-containing plants as food were raised by feeding the plants to the cultured mycelium by adding seed bacteria (= Bacteria Militaris) to a culture medium containing a culture made by diluting collagen powder. It is characterized by inoculating the host (brown rice), and as a result, in addition to the collagen component consumed by the host, when the Cordyceps sinensis spawn is cultured, collagen is ingested by the culture mixed with collagen powder, so that Cordyceps sinensis can further increase the collagen component in the future. It has the effect of making it more inclusive.
또한, 콜라겐을 숙주를 통해서 자연스럽게 동충하초에 흡수되도록 함으로써 별도의 장치가 더 필요하지 않고, 재배방법이 어렵지 않으므로 동충하초의 재배가 극히 용이하면서 재배에 필요한 비용을 절감시킬 수 있다.In addition, by allowing collagen to be naturally absorbed into Cordyceps sinensis through the host, no additional equipment is needed and the cultivation method is not difficult, making it extremely easy to cultivate Cordyceps sinensis and reducing the cost of cultivation.
이하, 본 발명을 보다 상세히 설명하도록 한다.Hereinafter, the present invention will be described in more detail.
본 발명에 따른 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법에 관한 것으로,It relates to a method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin according to the present invention,
혼합물을 호기발효 및 발효하여 숙주의 먹이로 조성하는 제1공정;A first step of aerobic fermentation and fermentation of the mixture to form food for the host;
상기 먹이를 사육배지로 하여 채란한 후 유충으로 성장시키는 제2공정;A second process of collecting eggs using the food as a breeding medium and growing them into larvae;
상기 유충을 급냉시켜 숙주로 완성하는 제3공정;A third process of rapidly freezing the larvae and completing them as hosts;
콜라겐 분말을 희석시켜 제조한 배양물에 종균을 투입하여 배양하여 균사체를 준비하는 제4공정; 및A fourth step of preparing mycelium by adding seed to a culture prepared by diluting collagen powder and culturing it; and
상기 준비된 균사체를 숙주에 접종하고 습도를 유지하여 동충하초를 재배하는 제5공정;을 포함하는 것을 특징으로 한다.A fifth step of cultivating Cordyceps sinensis by inoculating the prepared mycelium into the host and maintaining humidity.
본 발명에 따른 재배방법은 동충하초에 함유되도록 하고자 하는 특성성분이 함유된 필수대상물이 포함되는 먹이로 사육하여 키운 숙주를 급냉시켜서 종균을 접종하여 재배하는 것을 특징으로 한다.The cultivation method according to the present invention is characterized by rapidly freezing hosts grown on food containing essential substances containing the characteristic components desired to be contained in Cordyceps sinensis and cultivating them by inoculating them with spawn.
본 발명에 따른 재배 방법은 3℃ 이하의 극저온 충격을 주기적으로 가함으로써, 높은 함량의 코디세핀을 함유하는 것을 특징으로 하며, 구체적으로는 동충하초균을 포함하는 현미 배지 혼합물을 3℃ 이하의 제1온도 및 20℃ 이상의 제2온도에서 순차적으로 배양하는 저온 충격 주기가 반복 수행되는 배양 단계를 포함한다. The cultivation method according to the present invention is characterized by containing a high content of cordycepin by periodically applying cryogenic shock below 3°C, and specifically, the brown rice medium mixture containing Cordyceps sinensis is grown in the first culture medium at 3°C or below. It includes a culturing step in which a low-temperature shock cycle of sequential culturing at a temperature and a second temperature of 20° C. or higher is repeatedly performed.
상기 “극저온 충격”은 동충하초 재배 시 동충하초균의 3℃ 이하의 온도를 가하는 수단과 17℃ 이상의 온도차를 가하는 수단을 포함하는 것을 의미한다. 상기 극저온 충격을 주기 위한 수단으로 상기 제1온도와 상기 제2온도에서 순차적으로 동충하초균을 배양하는 저온 충격 주기를 예시할 수 있다. 구체적으로, 상기 저온 충격 주기는, t1) 상기 현미 배지 혼합물이 상기 제1온도로 감온되는 단계, t2) 상기 감온된 현미 배지 혼합물이 상기 제2온도로 승온되는 단계 및 t3) 상기 승온된 현미 배지 혼합물이 상기 제2온도로 유지되는 단계를 포함한다.The “cryogenic shock” means including a means of applying a temperature of 3°C or lower to the Cordyceps fungi when cultivating Cordyceps sinensis and a means of applying a temperature difference of 17°C or higher. As a means for applying the cryogenic shock, a low-temperature shock cycle in which Cordyceps sinensis is sequentially cultured at the first temperature and the second temperature may be used. Specifically, the low-temperature shock cycle includes t1) reducing the temperature of the brown rice medium mixture to the first temperature, t2) increasing the temperature of the reduced brown rice medium mixture to the second temperature, and t3) raising the temperature of the brown rice medium mixture. and maintaining the mixture at the second temperature.
상기 제1온도는 3℃ 이하, 바람직하게는 1℃ 이하인 것이 좋으며, 이때 하한 값은 -2℃일 수 있다. 바람직한 일 예로, 상기 제1온도가 -2 내지 1℃로 설정될 경우, 동충하초균에 극저온 충격이 높은 확률로 가해짐으로써, 극저온 충격이 가해지지 않은 경우와 비교하여 균의 면역력 및 환경 적응력이 현저히 향상되어 코디세핀 함유량이 더욱 증대된 현미 동충하초를 재배할 수 있다.The first temperature is preferably 3°C or lower, preferably 1°C or lower, and the lower limit may be -2°C. As a preferred example, when the first temperature is set to -2 to 1°C, a cryogenic shock is applied to the Cordyceps sinensis with a high probability, so that the immunity and environmental adaptability of the bacteria are significantly increased compared to the case where the cryogenic shock is not applied. It is possible to cultivate brown rice Cordyceps sinensis with improved cordycepin content.
상기 제2온도는 20℃ 이상, 구체적으로 20 내지 30℃일 수 있다. 상기 제2온도가 20℃ 미만으로 설정될 경우, 상기 제1온도에서 극저온 상태를 유발하더라도 17℃ 이상의 온도 편차가 가해지지 않아 극저온 충격이 완화될 수 있어, 전술한 코디세핀 함유량의 증대를 기대할 수 없다.The second temperature may be 20°C or higher, specifically 20 to 30°C. When the second temperature is set to less than 20°C, even if a cryogenic state is induced at the first temperature, a temperature deviation of 17°C or more is not applied, so the cryogenic shock can be alleviated, and an increase in the cordycepin content described above can be expected. does not exist.
상기 t1) 단계는 1 내지 3 일, 구체적으로 1.5 내지 2.5 일 동안 수행되는 것이 바람직할 수 있다. 이를 만족할 경우, 동충하초균에 극저온 충격이 신속히 원활하게 가해지면서도, 극저온 충격이 장시간 가해짐에 따른 동충하초균의 생육 저하 또는 불활성을 방지할 수 있다.The step t1) may be preferably performed for 1 to 3 days, specifically 1.5 to 2.5 days. If this is satisfied, it is possible to quickly and smoothly apply a cryogenic shock to the Cordyceps sinensis bacteria and prevent a decrease in the growth or inactivation of the Cordyceps sinensis bacteria due to a prolonged cryogenic shock being applied.
상기 t2) 단계는 3 내지 7 일, 구체적으로 4 내지 6 일 동안 수행되는 것이 바람직할 수 있고, 상기 t3) 단계는 5 내지 10 일, 구체적으로 6 내지 8 일 동안 수행되는 것이 바람직할 수 있다. 이를 만족할 경우, 온도 편차에 의한 극저온 충격이 효과적으로 가해지면서도 적절한 생육 온도의 간헐적 부여에 따른 동충하초균의 생육 저하 또는 불활성을 방지할 수 있다.Step t2) may be preferably performed for 3 to 7 days, specifically 4 to 6 days, and step t3) may be preferably performed for 5 to 10 days, specifically 6 to 8 days. If this is satisfied, it is possible to effectively apply cryogenic shock due to temperature deviation and prevent growth deterioration or inactivation of Cordyceps sinensis due to intermittent provision of an appropriate growth temperature.
상기 t1) 단계는 상기 제1온도로 감온되는 과정 및 상기 제1온도를 유지하는 과정을 포함할 수 있다. 상기 제1온도로 감온되는 과정은 급격한 온도 감소 과정인 것이 바람직할 수 있으며, 구체적으로 상기 과정에서 감온속도는 1 내지 10℃/min일 수 있다. 하지만 이는 바람직한 일 예로서 설명된 것일 뿐, 본 발명이 이에 반드시 제한되어 해석되는 것은 아니다.The step t1) may include a process of reducing the temperature to the first temperature and a process of maintaining the first temperature. It may be preferable that the process of reducing the temperature to the first temperature is a process of rapid temperature reduction. Specifically, the temperature reduction rate in the process may be 1 to 10°C/min. However, this is only described as a preferred example, and the present invention is not necessarily limited thereto.
상기 t2) 단계는 상기 제2온도로 승온되는 과정 및 상기 제2온도를 유지하는 과정을 포함할 수 있다. 상기 제2온도로 승온되는 과정은 급격한 온도 증가 과정인 것이 바람직할 수 있으며, 구체적으로 상기 과정에서 승온속도는 0.01 내지 0.5℃/min일 수 있다. 하지만 이는 바람직한 일 예로서 설명된 것일 뿐, 본 발명이 이에 반드시 제한되어 해석되는 것은 아니다.The step t2) may include a process of increasing the temperature to the second temperature and maintaining the second temperature. It may be preferable that the process of raising the temperature to the second temperature is a process of rapid temperature increase. Specifically, the temperature increase rate in the process may be 0.01 to 0.5°C/min. However, this is only described as a preferred example, and the present invention is not necessarily limited thereto.
상기 t3) 단계는 상기 t2) 단계의 제2온도를 일정 시간 지속적으로 유지하는 단계이다. 이러한 t3) 단계를 더 거칠 경우, 극저온 충격이 가해지면서도 적절한 생육 온도의 간헐적 부여에 따른 동충하초균의 생육 저하 또는 불활성을 방지할 수 있다.The step t3) is a step of continuously maintaining the second temperature of the step t2) for a certain period of time. If this step t3) is further performed, it is possible to prevent growth deterioration or inactivation of Cordyceps sinensis due to intermittent provision of an appropriate growth temperature even when cryogenic shock is applied.
상기 저온 충격 주기는 2 회 이상, 좋게는 3 회 이상, 구체적으로 2 내지 4 회 수행되는 것이 바람직하다. 이를 만족할 경우, 동충하초균에 극저온 충격이 주기적으로 가해짐으로써, 1 회만 수행된 경우와 비교하여 균의 면역력 및 환경 적응력이 현저히 향상되어 코디세핀 함유량이 더욱 증대된 현미 동충하초를 재배할 수 있다.The low-temperature shock cycle is preferably performed two or more times, preferably three or more times, and specifically 2 to 4 times. If this is satisfied, by periodically applying cryogenic shock to the Cordyceps sinensis, the immunity and environmental adaptability of the bacteria are significantly improved compared to the case where it is performed only once, making it possible to cultivate brown rice Cordyceps sinensis with a further increased cordycepin content.
상기 배양 단계에서, 배양은 상대습도 50 내지 80%에서 수행되는 것이 바람직할 수 있다. 이를 만족할 경우, 전술한 저온 충격 주기가 반복 수행됨에 따른 동충하초균의 생육이 저해되는 문제를 방지하고, 원활한 생육 환경을 제공할 수 있다.In the above culturing step, it may be desirable for the culturing to be performed at a relative humidity of 50 to 80%. If this is satisfied, it is possible to prevent the problem of the growth of Cordyceps sinensis being inhibited as the above-described low-temperature shock cycle is repeatedly performed, and to provide a smooth growth environment.
상기 배양 단계 이전에, 상기 현미 배지 혼합물을 제조하는 단계를 더 포함할 수 있다. 이때 상기 현미배지 혼합물을 제조하는 단계는 현미 배지 제조 단계, 종균 혼합 배지 제조 단계, 상기 종균 혼합 배지를 상기 현미 배지에 접종하여 현미 배지 혼합물을 제조하는 단계를 포함할 수 있다. Before the culturing step, the step of preparing the brown rice medium mixture may be further included. At this time, the step of preparing the brown rice medium mixture may include the step of preparing the brown rice medium, the step of preparing the seed mixture medium, and the step of inoculating the seed mixture medium into the brown rice medium to prepare the brown rice medium mixture.
이하, 각 공정 단계의 구성을 설명한다.Hereinafter, the configuration of each process step will be described.
제1공정(먹이조성단계)1st process (food composition stage)
제1공정에서는 혼합물을 호기발효 및 발효하여 숙주의 먹이로 조성할 수 있다.In the first process, the mixture can be aerobically fermented and fermented to form food for the host.
상기 혼합물은 식물 20-30 중량%, 참나무톱밥 40-50 중량%, 유용미생물 1-3 중량%, 당밀 4-6 중량%, 대두박 5-10 중량%, 밀기울 5-10 중량% 및 물 4-12 중량%을 혼합하여 제조되는 것이 바람직하다.The mixture includes 20-30% by weight of plants, 40-50% by weight of oak sawdust, 1-3% by weight of useful microorganisms, 4-6% by weight of molasses, 5-10% by weight of soybean meal, 5-10% by weight of wheat bran, and 4-4% of water. It is preferably prepared by mixing 12% by weight.
상기 발효는 5주간 1차 호기발효 후 2차, 3차 발효는 4주 간격으로 수분을 공급해서 배합해주어 추가적인 호기발효가 일어나도록 하여 사육도중 발효열의 발생을 최소화시켜 숙주의 먹이로 조성토록 하는 것이 바람직하다.In the above fermentation, the first aerobic fermentation lasts for 5 weeks, and then the second and third fermentations are mixed by supplying moisture at four-week intervals to allow additional aerobic fermentation to occur, thereby minimizing the generation of fermentation heat during rearing and forming it into food for the host. desirable.
상기 식물은 아욱과 아욱, 아욱종자 동규자, 마시멜로, 무궁화과 무궁화, 목근피, 닥풀, 수백풀, 백오동, 두충과, 화살나무과, 노박덩굴, 화살나무, 사철나무, 참빗살나무, 금화규, 신선초, 오크라, 블로키아, 목화, 부용차, 느릅나무, 유근피, 보리수나무, 알테아, 담배(Tabacco), 옥수수(Maize), 자주개자리(Alfalfa), 벼(Rice), 감자(Potato), 대두(Soybean), 토마토(tomato), 밀(Wheat), 보리(Barley), 캐놀라(Canola), 당근(Carrot) 및 목화(Cotton) 등을 분쇄 및 추출한 것을 사용하는 것이 바람직하나, 콜라겐이 함유된 식물이라면 이에 제한되는 것은 아니다.The above plants include Malvaceae and Malvaceae, Malvaceae seeds, Marshmallow, Rose of Sharon from the Rose of Sharon family, Rose of Sharon from the Rose of Sharon family, Root bark of the Rose of Sharon, Mulberry mulberry, Hundreds of grass, Eucalyptus officinalis, Euphyllaceae, Arrowaceae, Argentine vine, Arrowroot tree, Perpendicularis tree, Siberian chinensis, Geumhwagyu, Sinseoncho, Okra, Blochia, Cotton, Buyongcha, Elm, Radix Bark, Bodhi Tree, Althea, Tabacco, Maize, Alfalfa, Rice, Potato, Soybean ), tomatoes, wheat, barley, canola, carrots, and cotton, etc., are crushed and extracted. However, if it is a plant containing collagen, this is not recommended. It is not limited.
상기 숙주는 벌, 개미, 잠자리, 나비, 매미, 노린재, 딱정벌레, 파리, 거미, 귀뚜라미로 이루어질 수 있으나, 상기 숙주로는 일명 꽃뱅이라고 불리는 흰점박이꽃무지를 사용하는 것이 바람직하다. The hosts may include bees, ants, dragonflies, butterflies, cicadas, stink bugs, beetles, flies, spiders, and crickets. However, it is preferable to use white-spotted flower beetles, also known as flower beetles, as the hosts.
본 발명의 일 실시형태에서, 상기 콜라겐이 함유된 식물 대신 달팽이 점액 추출물을 사용하거나 상기 식물에 달팽이 점액 추출물을 혼합하여 사용하는 것을 특징으로 한다. 이때, 달팽이 점액 추출물을 포함하는 혼합물은 콜라겐을 함유하는 식물의 함량과 동일하게 20-30 중량%으로 포함되는 것이 바람직하다.In one embodiment of the present invention, snail mucus extract is used instead of the plant containing the collagen, or the plant is mixed with the snail mucus extract. At this time, the mixture containing the snail mucus extract is preferably contained at 20-30% by weight, the same as the content of the collagen-containing plant.
상기 달팽이 점액 추출물은 천연물로서 당단백질(glycoprotein)과 뮤코다당(mucopolysaccharide)을 포함하는 세포 분비물이며, 뮤신(mucin), 알란토인(allantoin), 글리콜산(glycolic acid), 콜라겐(collagen) 및 엘라스틴(elastin)이 들어있다.The snail mucus extract is a natural product and is a cell secretion containing glycoprotein and mucopolysaccharide, and contains mucin, allantoin, glycolic acid, collagen, and elastin ( elastin) is included.
상기 달팽이 점액 추출물을 수득하는 방법은 달팽이로부터 점액을 분리한 후, 상온 또는 20-40℃에서 점액에 충분한 양의 추출 용매를 넣고 흔들어 주면서 반응이 일어나도록 한다. 공지된 추출 방법을 모두 사용할 수 있지만 고온의 열수 추출법은 단백질의 변성 내지 기능 저하를 발생시킬 수 있어 바람직하지 않다.The method of obtaining the snail mucus extract is to separate the mucus from the snail, add a sufficient amount of extraction solvent to the mucus at room temperature or 20-40°C, and shake to allow the reaction to occur. All known extraction methods can be used, but high-temperature hot water extraction is not preferable because it can cause denaturation or functional deterioration of proteins.
상기 추출 용매로는 수성 용매(예를 들면, 물, 산성 또는 염기성의 수성 용매 등), 알코올류(예를 들면, 메탄올, 무수 에탄올, 에탄올 등의 저급 알코올, 혹은 프로필렌 글리콜, 1,3-부틸렌 글리콜 등의 다가 알코올 등), 아세톤 등의 케톤류 등의 1종 또는 2종류 이상을 적당히 혼합해서 사용할 수 있다. The extraction solvent includes aqueous solvents (e.g., water, acidic or basic aqueous solvents, etc.), alcohols (e.g., methanol, anhydrous ethanol, lower alcohols such as ethanol, or propylene glycol, 1,3-butyl). Polyhydric alcohols such as ren glycol, etc.), ketones such as acetone, etc. can be used alone or in an appropriate mixture of two or more types.
또한, 적당히 산, 알칼리 또는 완충제 등에 의해 추출 용매를 원하는 pH로 조정하는 것도 가능하다. 추출은 보통은, pH 2 내지 pH 13의 범위에서 실행되며, pH 4 내지 pH 10의 범위가 바람직하고, pH 4 내지 pH 7.5의 범위가 특히 바람직하다.Additionally, it is also possible to appropriately adjust the extraction solvent to the desired pH using an acid, alkali, or buffer. Extraction is usually carried out in the range of pH 2 to pH 13, with the range of pH 4 to pH 10 being preferred, and the range of pH 4 to pH 7.5 being particularly preferred.
달팽이 점액 추출물은 달팽이 점액에서 추출한 액상 또는 이를 건조하여 제조한 과립, 분말 또는 정제수에 희석한 용액 형태로 사용될 수 있다.Snail mucus extract can be used in the form of a liquid extracted from snail mucus, granules prepared by drying it, powder, or a solution diluted in purified water.
제2공정(채란단계 및 숙주사육단계)2nd process (egg picking stage and host breeding stage)
제2공정에서는 상기 제1공정에서 제조된 먹이를 사육배지로 하여 채란한 후 유충으로 성장시킬 수 있다. 구체적으로 제2공정은 채란단계, 숙주사육단계 및 숙주완성단계로 구성될 수 있다.In the second process, the food prepared in the first process can be used as a breeding medium to collect eggs and then grow into larvae. Specifically, the second process may consist of an egg picking stage, a host breeding stage, and a host completion stage.
먼저, 상기 제1공정을 거쳐서 조성된 먹이를 사육배지로 하여 65%의 함수량에 맞추어 수분을 조절한 후, 10kg을 계량하여 36L의 상자에 넓게 깔아두고 원활한 채란을 위해 사육실의 온도는 28℃로 설정하는 것이 바람직하다. 상기 사육배지에 숙주의 성충 60마리를 일주일간 투입하여 성충으로부터 300~400개의 알을 받도록 한 채란단계를 구비하는 것이 바람직하다.First, the food prepared through the above first process was used as a breeding medium and the moisture was adjusted to 65% water content. Then, 10 kg was weighed and spread out in a 36L box. The temperature of the breeding room was set to 28°C for smooth egg picking. It is desirable to set It is preferable to provide an egg picking step in which 60 adult hosts are introduced into the breeding medium for a week and 300 to 400 eggs are received from the adults.
그런 다음, 상기 채란단계를 거쳐서 받은 알이 부화되면 20-28℃의 온도와 65%의 습도에서 13주 동안 먹이를 교체하면서 사육하여 1령의 유충을 3령 말기의 유충으로 성장토록 하는 숙주사육단계를 구비하는 것이 바람직하다.Then, when the eggs received through the above egg-laying stage hatch, they are reared at a temperature of 20-28°C and humidity of 65% for 13 weeks while changing the food, and the 1st instar larvae are grown into late 3rd instar larvae. It is desirable to have steps.
제3공정(숙주완성단계)Third process (host completion stage)
제3공정에서는 상기 제2공정에서 성장된 유충을 급냉시켜 숙주로 완성할 수 있다.In the third process, the larvae grown in the second process can be rapidly frozen to complete the host.
구체적으로, 상기 제2공정의 숙주사육단계를 거쳐서 얻어진 완전히 성장된 유충을 급냉시켜서 숙주를 완성하는 숙주완성단계를 구비하는 것이 바람직하다.Specifically, it is preferable to provide a host completion step in which the fully grown larvae obtained through the host breeding step of the second process are rapidly frozen to complete the host.
제4공정(균사체 준비단계)4th process (mycelium preparation step)
제4공정에서는 콜라겐 분말을 희석시켜서 만든 배양물에 종균을 투입하여 균사체로 배양토록 하여 균사체 준비할 수 있다.In the fourth process, mycelium can be prepared by adding seed to a culture made by diluting collagen powder and culturing it into mycelium.
숙주와 별개로 콜라겐 추출물을 희석시켜서 만든 배양물에 종균을 투입하여 균사체로 배양토록 하되, 이와 같이 본 발명은 숙주의 먹이로 콜라겐이 함유된 식물을 먹이는 방법과 종균에 콜라겐이 희석된 배양물을 공급하여 2중으로 콜라겐 성분을 동충하초로 제공할 수 있다.Separately from the host, the seed is added to a culture made by diluting the collagen extract and cultured into mycelium. As such, the present invention includes a method of feeding plants containing collagen as food to the host and a culture in which collagen is diluted to the seed. By supplying it, the collagen component can be provided twice as Cordyceps sinensis.
상기 종균은 밀리타리스(Cordyceps militaris)균을 사용하는 것이 바람직하나, 이에 제한되는 것은 아니다.It is preferable to use Cordyceps militaris as the starter, but it is not limited thereto.
제5공정(접종단계 및 동충하초 재배단계)5th process (inoculation stage and Cordyceps sinensis cultivation stage)
제5공정에서는 상기 제3공정에서 급냉된 숙주에 상기 제4공정에서 얻어진 균사체를 접종한 후, 습도를 유지하여 동충하초를 재배할 수 있다.In the fifth process, the host quenched in the third process is inoculated with the mycelium obtained in the fourth process, and then Cordyceps sinensis can be cultivated by maintaining humidity.
구체적으로, 상기 숙주완성단계에서 급냉된 숙주에 균사체준비단계에서 얻어진 균사체를 접종하여 개별통에 100g씩 나누어 넣고 20℃에서 종균이 성장하도록 수분을 공급함과 동시에 광을 조사하는 접종단계를 구비하며, 상기 접종단계를 거쳐서 숙주에 접종된 종균이 자라기 시작하면서 15-20일이 경과 후 재배실의 온도를 18℃로 조정하고, 60-65일까지의 성장기를 경과할 때까지 수분을 분무기를 통하여 투여해서 습도를 65%로 유지하면서 동충하초를 재배토록 하는 동충하초 재배단계를 구비하는 것이 바람직하다. Specifically, the host rapidly frozen in the host completion step is inoculated with the mycelium obtained in the mycelium preparation step, divided into 100g each container, and provided with an inoculation step of supplying moisture and irradiating light at the same time to allow the spawn to grow at 20°C. After the above inoculation step, the seed inoculated into the host begins to grow. After 15-20 days, the temperature in the cultivation room is adjusted to 18°C, and moisture is administered through a sprayer until the growth period of 60-65 days has passed. Therefore, it is desirable to provide a Cordyceps sinensis cultivation step to cultivate Cordyceps sinensis while maintaining humidity at 65%.
본 발명의 일 실시형태에서, 상기 종균이 자라기 시작하는 경우, 기능성 증강제를 추가 투입하여 재배되는 동충하초가 고함량의 콜라겐 및 코디세핀을 함유하도록 할 수 있다.In one embodiment of the present invention, when the seed starts to grow, a functional enhancer can be added so that the cultivated Cordyceps sinensis contains a high content of collagen and cordycepin.
본 발명은 상기 방법을 통해 재배된 동충하초로부터 콜라겐 및 코디세핀을 추출하는 방법에 관한 것으로, The present invention relates to a method for extracting collagen and cordycepin from Cordyceps sinensis cultivated through the above method,
상기 동충하초, 물 및 탄수화물분해효소를 혼합한 후 1차 추출하는 공정;A process of mixing the Cordyceps sinensis, water, and carbohydrate-decomposing enzyme and then performing primary extraction;
상기 1차 추출물을 냉각시킨 후 섬유질분해효소 및 단백질분해효소를 첨가한 후 2차 추출하는 공정; 및A process of cooling the primary extract, adding fibrous enzyme and proteolytic enzyme, and performing secondary extraction; and
실활 과정을 거쳐 여과 및 농축하는 공정;을 포함하는 것을 특징으로 한다.It is characterized by including a process of filtration and concentration through a deactivation process.
본 발명의 일 실시형태에서, 상기 추출 방법은 저온고압효소처리방법을 이용하는 것을 특징으로 한다.In one embodiment of the present invention, the extraction method is characterized by using a low-temperature and high-pressure enzyme treatment method.
구체적으로, 상기 추출 방법은 Specifically, the extraction method is
동충하초 100~200g을 넣고 물 300~600g을 넣은 후 탄수화물분해효소(알파-아밀라아제) 0.5~1%을 첨가한 후 70~80℃에서 60~90분 정도 1차 추출을 수행하고, 50~60℃까지 냉각 후 섬유질분해효소(셀룰라아제) 0.5~1%, 단백질분해효소(프로테아제) 1~2%를 넣은 후 50~60℃에서 2차 추출하며, 90~100℃의 실활 과정을 거친 후 여과, 농축을 진행하는 것이 바람직하다.Add 100-200g of Cordyceps sinensis, add 300-600g of water, add 0.5-1% of carbohydrate decomposition enzyme (alpha-amylase), and perform primary extraction at 70-80℃ for 60-90 minutes, then 50-60℃. After cooling, 0.5-1% of cellulase (cellulase) and 1-2% of proteolytic enzyme (protease) are added, followed by secondary extraction at 50-60℃, followed by deactivation at 90-100℃, followed by filtration and concentration. It is desirable to proceed.
상기 추출방법은 물(증류수), 에탄올 등을 사용할 수 있으나, 물을 사용하는 것이 보다 바람직하다.The extraction method may use water (distilled water), ethanol, etc., but it is more preferable to use water.
이하, 실시예에 의해 본 발명을 보다 구체적으로 설명하고자 한다. 이들 실시예는 오직 본 발명을 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 국한되지 않는다는 것은 당업자에게 있어서 자명하다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it is obvious to those skilled in the art that the scope of the present invention is not limited to these examples.
실시예 1 : 코디세핀의 함량분석Example 1: Content analysis of cordycepin
본 발명의 방법에 따라 재배한 동충하초의 코디세핀 함량을 다음과 같은 방법으로 분석하였다. The cordycepin content of Cordyceps sinensis grown according to the method of the present invention was analyzed by the following method.
먼저, 종균을 접종한 후 60일 동안 재배한 동충하초 중 각 10개의 자실체만을 취하여 동결건조시켰다. 건조된 동충하초의 자실체 10g을 정제수 190g에 분산시키고 80℃에서 2시간 동안 추출하고 원심분리한 후 상등액을 여과하여 분석시료로 사용하였다. 코디세핀 함량은 HPLC를 이용하여 측정하였다. First, after inoculation with the spawn, only 10 fruiting bodies from Cordyceps sinensis grown for 60 days were taken and freeze-dried. 10 g of dried fruiting bodies of Cordyceps sinensis were dispersed in 190 g of purified water, extracted at 80°C for 2 hours, centrifuged, and the supernatant was filtered and used as an analysis sample. Cordycepin content was measured using HPLC.
측정 결과, 종래 방식으로 재배된 동충하초의 경우 15 내지 30 ppm의 코디세핀 함량이 포함된 반면, 본 발명의 방법에 따라 재배된 동충하초의 경우 400 내지 600 ppm의 코디세핀 함량이 포함된 것을 확인하였다.As a result of the measurement, it was confirmed that Cordyceps sinensis cultivated in a conventional manner contained a cordycepin content of 15 to 30 ppm, while Cordyceps sinensis cultivated according to the method of the present invention contained a cordycepin content of 400 to 600 ppm.
또한, 본 발명의 방법에 따라 재배된 동충하초의 경우, 종래 방식으로 재배된 동충하초 대비 저온 충격 주기 횟수가 3 배 더 증가됨에 따라 코디세핀 함유량이 500 내지 580 ppm으로 더 높게 나타나는 것을 확인하였다.In addition, in the case of Cordyceps sinensis grown according to the method of the present invention, it was confirmed that the cordycepin content was higher at 500 to 580 ppm as the number of cold shock cycles was increased by three times compared to Cordyceps sinensis cultivated by the conventional method.
실시예 2 : 콜라겐의 함량분석Example 2: Collagen content analysis
본 발명의 방법에 따라 재배한 동충하초의 콜라겐 함량을 다음과 같은 방법으로 분석하였다. 콜라겐의 주요 구성 아미노산인 L-프롤린 함량을 분석하였다.The collagen content of Cordyceps sinensis grown according to the method of the present invention was analyzed by the following method. The content of L-proline, a major amino acid in collagen, was analyzed.
상기 실시예 1과 동일한 분석시료를 대상으로 하였고, 분석시료 1mL에 3% 설포살리실산 4mL을 혼합하여 초음파 분산기에 5분간 방치하였다. 상기 혼합액 2mL에 산성 닌히드린 용액(닌히드린 2.5g /glacial acetic acid[빙초산] 6:인산 3:증류수 1[V:V] 100mL) 2mL, 빙초산 2mL을 혼합하여 95±5℃ 이상에서 1시간 방치 후 4℃ 이하에서 반응을 종결시키고 톨루엔 4mL을 혼합하여 520nm에서 흡광도를 측정하였다. L-프롤린(Proline)을 표준용액으로 하여, 시료 중 프롤린 함량을 측정하였다.The same analysis sample as in Example 1 was used, and 4 mL of 3% sulfosalicylic acid was mixed with 1 mL of the analysis sample and placed in an ultrasonic disperser for 5 minutes. Mix 2 mL of acidic ninhydrin solution (ninhydrin 2.5 g/glacial acetic acid [glacial acetic acid] 6:phosphoric acid 3:distilled water 1 [V:V] 100 mL) and 2 mL of glacial acetic acid in 2 mL of the above mixture and leave for 1 hour at above 95±5°C. The reaction was terminated below 4°C, 4 mL of toluene was mixed, and the absorbance was measured at 520 nm. Using L-proline as a standard solution, the proline content in the sample was measured.
그 결과, 종래 방식으로 재배된 동충하초의 경우 120 내지 130 ppm의 프롤린 함량이 포함된 반면, 본 발명의 방법에 따라 재배된 동충하초의 경우 180 내지 200 ppm의 프롤린 함량이 포함된 것을 확인하였다.As a result, it was confirmed that Cordyceps sinensis grown conventionally contained a proline content of 120 to 130 ppm, while Cordyceps sinensis cultivated according to the method of the present invention contained a proline content of 180 to 200 ppm.
따라서, 본 발명의 따른 재배방법을 통해 상기 콜라겐 사료와 콜라겐 종균으로 콜라겐 배지에서 충분히 공급받은 동충하초는 콜라겐 성분이 풍부하여 코디세핀의 고단위 기능성 식품이 될 수 있음을 확인하였다.Therefore, it was confirmed that Cordyceps sinensis sufficiently supplied in a collagen medium with the collagen feed and collagen starter through the cultivation method according to the present invention is rich in collagen components and can be a functional food with a high unit of cordycepin.
실시예 3 : 콜라겐 및 코디세핀의 추출 효과Example 3: Extraction effect of collagen and cordycepin
본 발명의 방법에 따라 재배한 동충하초를 저온고압효소처리방법을 통해 추출하여 추출물을 수득하였다. 수득한 추출물의 콜라겐 및 코디세핀 함량을 고성능 액체 크로마토그래피(HPLC)를 통해 측정하였으며, 그 결과를 하기 표 1에 나타내었다.An extract was obtained by extracting Cordyceps sinensis grown according to the method of the present invention through a low-temperature and high-pressure enzyme treatment method. The collagen and cordycepin contents of the obtained extract were measured using high-performance liquid chromatography (HPLC), and the results are shown in Table 1 below.
시료sample 코디세핀 함량(%)Cordycepin content (%) 콜라겐 함량(%)Collagen content (%)
동충하초 물 추출물Cordyceps sinensis water extract 3.473.47 5.785.78
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 본 발명이 속한 기술분야에서 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아님은 명백하다. 본 발명이 속한 기술분야에서 통상의 지식을 가진 자라면 상기 내용을 바탕으로 본 발명의 범주 내에서 다양한 응용 및 변형을 행하는 것이 가능할 것이다.As the specific parts of the present invention have been described in detail above, it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. do. Anyone skilled in the art to which the present invention pertains will be able to make various applications and modifications within the scope of the present invention based on the above contents.
따라서, 본 발명의 실질적인 범위는 첨부된 특허청구범위와 그의 등가물에 의하여 정의된다고 할 것이다.Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.
본 발명에 따른 동충하초 재배방법은 동충하초가 콜라겐을 다량으로 함유할 수 있고, 동충하초 본연의 효능과 더불어 콜라겐의 효능도 동시에 갖도록 하는 효과를 가질 수 있다. 또한, 콜라겐을 숙주를 통해서 자연스럽게 동충하초에 흡수되도록 함으로써 별도의 장치가 더 필요하지 않고, 재배방법이 어렵지 않으므로 동충하초의 재배가 극히 용이하면서 재배에 필요한 비용을 절감시킬 수 있다.The method of cultivating Cordyceps sinensis according to the present invention can have the effect of allowing Cordyceps sinensis to contain a large amount of collagen and to have the efficacy of collagen in addition to the natural efficacy of Cordyceps sinensis. In addition, by allowing collagen to be naturally absorbed into Cordyceps sinensis through the host, no additional equipment is needed and the cultivation method is not difficult, making it extremely easy to cultivate Cordyceps sinensis and reducing the cost of cultivation.

Claims (6)

  1. 혼합물을 호기발효 및 발효하여 숙주의 먹이로 조성하는 제1공정;A first step of aerobic fermentation and fermentation of the mixture to form food for the host;
    상기 먹이를 사육배지로 하여 채란한 후 유충으로 성장시키는 제2공정;A second process of collecting eggs using the food as a breeding medium and growing them into larvae;
    상기 유충을 급냉시켜 숙주로 완성하는 제3공정;A third process of rapidly freezing the larvae and completing them as hosts;
    콜라겐 분말을 희석시켜 제조한 배양물에 종균을 투입하여 배양하여 균사체를 준비하는 제4공정; 및A fourth step of preparing mycelium by adding seed to a culture prepared by diluting collagen powder and culturing it; and
    상기 준비된 균사체를 숙주에 접종하고 습도를 유지하여 동충하초를 재배하는 제5공정;을 포함하는 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법.A cultivation method of Cordyceps sinensis containing a high content of collagen and cordycepin, comprising a fifth step of inoculating the prepared mycelium into a host and cultivating Cordyceps sinensis by maintaining humidity.
  2. 제 1 항에 있어서, 상기 재배 방법은 3℃ 이하의 극저온 충격을 주기적으로 가함으로써, 높은 함량의 코디세핀을 함유하는 것을 특징으로 하는, 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법.The cultivation method of Cordyceps sinensis containing a high content of collagen and cordycepin according to claim 1, wherein the cultivation method contains a high content of cordycepin by periodically applying cryogenic shock of 3° C. or lower.
  3. 제 1 항에 있어서, 상기 혼합물은 콜라겐이 함유된 식물 20-30 중량%, 참나무톱밥 40-50 중량%, 유용미생물 1-3 중량%, 당밀 4-6 중량%, 대두박 5-10 중량%, 밀기울 5-10 중량% 및 물 4-12 중량%을 혼합하여 제조되는 것을 특징으로 하는, 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법.The method of claim 1, wherein the mixture contains 20-30% by weight of collagen-containing plants, 40-50% by weight of oak sawdust, 1-3% by weight of useful microorganisms, 4-6% by weight of molasses, 5-10% by weight of soybean meal, A method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin, characterized in that it is prepared by mixing 5-10% by weight of wheat bran and 4-12% by weight of water.
  4. 제 1 항에 있어서, 숙주가 섭취한 콜라겐 성분 외에 동충하초의 종균이 배양될 시에도 콜라겐 분말이 혼합된 배양물에 의해서 콜라겐이 섭취되어 재배되는 동충하초가 콜라겐 성분을 고함량으로 포함되는 것을 특징으로 하는, 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법.According to claim 1, in addition to the collagen component consumed by the host, even when the Cordyceps sinensis spawn is cultured, collagen is consumed by a culture mixed with collagen powder, and the cultivated Cordyceps sinensis contains a high content of collagen components. , Method of cultivating Cordyceps sinensis containing high content of collagen and cordycepin.
  5. 제1항에 있어서, 상기 혼합물은 달팽이 점액 추출물을 더 포함하는 것을 특징으로 하는, 고함량의 콜라겐 및 코디세핀을 함유하는 동충하초의 재배 방법.The method of cultivating Cordyceps sinensis containing a high content of collagen and cordycepin according to claim 1, wherein the mixture further comprises snail mucus extract.
  6. 제1항의 방법에 따라 재배된 동충하초, 물 및 탄수화물분해효소를 혼합한 후 1차 추출하는 공정;A process of mixing Cordyceps sinensis cultivated according to the method of paragraph 1, water, and carbohydrate decomposing enzyme and then performing primary extraction;
    상기 1차 추출물을 냉각시킨 후 섬유질분해효소 및 단백질분해효소를 첨가한 후 2차 추출하는 공정; 및A process of cooling the primary extract, adding fibrous enzyme and proteolytic enzyme, and performing secondary extraction; and
    실활 과정을 거쳐 여과 및 농축하는 공정;을 포함하는 것을 특징으로 하는, 동충하초로부터 고순도 콜라겐 및 코디세핀을 추출하는 방법.A method of extracting high purity collagen and cordycepin from Cordyceps sinensis, comprising a process of filtration and concentration through a deactivation process.
PCT/KR2023/019337 2022-11-28 2023-11-28 Method for cultivating cordyceps militaris with high content of collagen and cordycepin, and method for extracting high-purity collagen and cordycepin WO2024117733A1 (en)

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Citations (5)

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KR20030010112A (en) * 2001-07-25 2003-02-05 에이치엔엠바이오(주) A method of cultivating cordyceps based upon a snail or a mud snail
KR20170005935A (en) * 2015-07-06 2017-01-17 김철군 Medium composition for cultivating vegetable worms and the method of cultivating vegetable worms
KR20200048736A (en) * 2018-10-30 2020-05-08 주식회사 씨앤바이오 Vegetable worms using unpolished rice and its cultivation method
KR20210079100A (en) * 2019-12-19 2021-06-29 반정우 Cultivation method of Cordyceps sinensis containing collagen
KR20220081457A (en) * 2020-12-08 2022-06-16 영월고소애협동조합 Method for extracting cordycepine from Cordyceps cultivated as edible insects

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KR101347219B1 (en) 2011-11-24 2014-01-03 한라산동충하초영농조합법인 a Method of Cultivation Cordyceps Militaris

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KR20030010112A (en) * 2001-07-25 2003-02-05 에이치엔엠바이오(주) A method of cultivating cordyceps based upon a snail or a mud snail
KR20170005935A (en) * 2015-07-06 2017-01-17 김철군 Medium composition for cultivating vegetable worms and the method of cultivating vegetable worms
KR20200048736A (en) * 2018-10-30 2020-05-08 주식회사 씨앤바이오 Vegetable worms using unpolished rice and its cultivation method
KR20210079100A (en) * 2019-12-19 2021-06-29 반정우 Cultivation method of Cordyceps sinensis containing collagen
KR20220081457A (en) * 2020-12-08 2022-06-16 영월고소애협동조합 Method for extracting cordycepine from Cordyceps cultivated as edible insects

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