WO2024020592A1 - Cosmetic or personal care composition comprising recombinant milk protein - Google Patents

Cosmetic or personal care composition comprising recombinant milk protein Download PDF

Info

Publication number
WO2024020592A1
WO2024020592A1 PCT/US2023/070793 US2023070793W WO2024020592A1 WO 2024020592 A1 WO2024020592 A1 WO 2024020592A1 US 2023070793 W US2023070793 W US 2023070793W WO 2024020592 A1 WO2024020592 A1 WO 2024020592A1
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant
cosmetic
personal care
care composition
casein
Prior art date
Application number
PCT/US2023/070793
Other languages
French (fr)
Inventor
Bonney Oommen
Original Assignee
Perfect Day, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perfect Day, Inc. filed Critical Perfect Day, Inc.
Publication of WO2024020592A1 publication Critical patent/WO2024020592A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4717—Plasma globulins, lactoglobulin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/20—Milk; Whey; Colostrum

Definitions

  • the present invention relates generally to cosmetic or personal care compositions that comprise recombinant milk proteins.
  • the present invention further provides methods for producing such cosmetic or personal care compositions.
  • compositions comprise proteins derived from mammalian milk. These milk proteins impart or contribute to critical attributes and/or functionalities of such compositions.
  • a cosmetic or personal care composition comprising a milk protein component, wherein the milk protein component comprises or consists essentially of a recombinant milk protein.
  • the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be a single recombinant milk protein.
  • the single recombinant milk protein may be: a recombinant p-lactoglobulin, a recombinant a-lactalbumin, a recombinant GMP, a recombinant lactotransferrin, a recombinant bovine serum albumin, a recombinant lactoperoxidase, a recombinant K-casein, a recombinant -casein, a recombinant a-Sl -casein; or a recombinant a-S2-casein.
  • the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be a mixture of two or more recombinant milk proteins.
  • the mixture of two or more recombinant milk proteins may comprise: a recombinant P-lactoglobulin, a recombinant a-lactalbumin, a recombinant GMP, a recombinant lactotransferrin, a recombinant bovine serum albumin, a recombinant lactoperoxidase, a recombinant K-casein, a recombinant [3-casein, a recombinant a-Sl -casein; and/or a recombinant a-S2-casein.
  • the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be hydrolyzed.
  • the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be a multimer or polymer of recombinant milk protein monomers.
  • the recombinant milk protein may be obtained via production by a recombinant host cell.
  • the recombinant host cell may be a recombinant fungal host cell (e.g., recombinant yeast host cell, recombinant filamentous fungal host cell).
  • the recombinant fungal host cell may be derived from Saccharomyces, Kluyveromyces, Pichia, Tetrahymena, Yarrowia, Hansenula, Blastobotrys, Zygosaccharomyces, Debaryomyces, Aspergillus, Candida, Trichoderma, Fusarium, Chrysosporium, Physcomitrella, or Neurospora.
  • the method may further comprise polymerizing the recombinant milk protein.
  • SUBSTITUTE SHEET ( RULE 26) [0013]
  • the method comprises applying to a body surface a cosmetic or personal care composition of any of paragraphs [0007] through [0011], DETAILED DESCRIPTION OF THE INVENTION
  • x, y, and/or z may refer to “x” alone, “y” alone, “z” alone, “x, y, and z", “(x and y) or z", “(x and z) or y”, “(y and z) or x", "x and y” alone, “x and z” alone, “y and z” alone, or "x or y or z”.
  • At least one and “one or more” as used herein refer to one, two, three, four, five, six, seven, eight, nine, ten, at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, or more, or all of the elements subsequently listed.
  • body surface refers to the exposed area of a human body.
  • body surfaces include skin, hair, nail, tooth, and tissues of the oral cavity (e.g., gums).
  • casein refers to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least
  • SUBSTITUTE SHEET (RULE 26) 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of ammo acids in a casein natively found in a mammal-produced milk (i.e., a casein that is native to a mammal-produced milk; e.g., a native casein).
  • a casein natively found in a mammal-produced milk i.e., a casein that is native to a mammal-produced milk; e.g., a native casein.
  • caseins include P-casein, /-casein, K-casein, a-Sl-casein, and a-S2-casein.
  • P- casein”, “/-casein”, “K-casein”, “a-Sl -casein”, and “a-S2-casein” as used herein refer to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of amino acids in a P-casein, /-casein, K-casein, a- S
  • corresponding cosmetic or personal care composition refers to a cosmetic or personal care composition that is identical in composition to the cosmetic or personal care composition that is compared to the “corresponding cosmetic or personal care composition” except that the “corresponding cosmetic or personal care composition” comprises a corresponding native milk protein in place of the recombinant milk protein that is comprised in the cosmetic or personal care composition that is compared to the “corresponding cosmetic or personal care composition”.
  • corresponding native milk protein refers to a native milk protein that is identical in amino acid sequence to the recombinant milk protein that is compared to the “corresponding native milk protein” but that is obtained from a mammal- produced milk.
  • cosmetic or personal care composition refers to a composition that upon application to a body surface confers a perceived or actual beautifying
  • Non-limiting examples of cosmetic or personal care compositions include anti-wrinkling treatments (i.e., compositions used for tensioning [e.g., smoothing out of skin, reducing wrinkles in skin, removing fine lines in skm]), anti-aging treatments (i.e., compositions used for removing signs of aging [e.g., wrinkles, fine lines, manifestations of photodamage (e.g., sun spots)]), sun protection (i.e., compositions used to protect against UV exposure), anti-bum treatments (i.e., compositions used for soothing bums [e.g., sunbums]), anti-acne treatments (i.e., compositions that are effective in the treatment of acne and/or the symptoms associated therewith), skin cleansers (i.e., compositions used for cleaning skin and/or skin pores [e.g., nose strips for pore cleaning]), anti-dandruff treatments (i.
  • anti-wrinkling treatments i.e., compositions used for tensioning [
  • SUBSTITUTE SHEET (RULE 26) gums, mouthwashes, toothpastes, gum-cleaning agents, denture adhesives, denture fixatives, breath strips, floss mechanisms, teeth whitening strips).
  • the term "essentially free of' as used herein refers to the indicated component being either not detectable in the indicated composition by common analytical methods, or to the indicated component being present in such trace amount as to not be functional.
  • the term "functional” as used in this context refers to not materially contributing to properties of the composition comprising the trace amount of the indicated component, or to not having material activity (e.g., chemical activity, enzymatic activity) in the indicated composition comprising the trace amount of the indicated component, or to not having health-adverse effects upon use or consumption of the composition comprising the trace amount of the indicated component.
  • materially contributing refers to the indicated component contributing to an attribute of a composition to such extent that in the absence of the component (e.g., in a reference composition that is identical to the composition except that it lacks the indicated component) the attribute is at least 10%, at least 20%, at least 30%, at least 40%, or at least 50% less present/active/measurable.
  • filamentous fungus refers to an organism from the filamentous form of the subdivision Eumycota and Oomycota (as defined by Hawksworth et al., In, Ainsworth and Bisby's Dictionary of The Fungi, 8th edition, 1995, CAB International, University Press, Cambridge, UK).
  • a filamentous fungus is distinguished from a yeast by its hyphal elongation during vegetative growth.
  • fungus refers to organisms of the phyla Ascomycotas, Basidiomycota, Zygomycota, and Chythridiomycota, Oomycota, and Glomeromycota. It is understood, however, that fungal taxonomy is continually evolving, and therefore this specific definition of the fungal kingdom may be adjusted in the future.
  • host cell refers not only to the particular subject cell but also to the progeny of such cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the subject cell, but are still included within the scope of the term “host cell” as used herein.
  • identity refers to the nucleotide or amino acid residues that are the same when the two or more polynucleotide or polypeptide sequences, respectively, are aligned for maximum correspondence.
  • the "identity” may exist over a region of the sequences being compared (e.g., over the length of a functional domain) or over
  • SUBSTITUTE SHEET (RULE 26) the full length of the sequences.
  • a “region” is considered to be a continuous stretch of at least 6, 9, 14, 19, 24, 29, 34, 39, or more nucleotides, or of at least 2, 6, 10, 14, 18, 22, 26, 30, or more amino acids.
  • typically one sequence acts as a reference sequence to which one or more test sequences are compared.
  • test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary', and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters.
  • Optimal alignment of sequences for comparison may be conducted, e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Sequence Analysis Software Package of the Genetics Computer Group (GCG), University of Wisconsin Biotechnology Center, which may be used with default parameters), or by visual inspection (see generally Ausubel et al., infra).
  • BLAST Altschul et al. 1990 J. Mol. Biol. 215:403-410; Gish & States. 1993 Nature Genet. 3:266-272; Madden et al. 1996 Meth. Enzymol. 266:131-141; Altschul et al. 1997 Nucleic Acids Res. 25:3389-3402; Zhang 7 Madden. 1997 Genome Res. 7:649-656).
  • Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information.
  • the percent sequence identity or degree of homology may be adjusted upwards to correct for the conservative nature of the substitution. Means for making this adjustment are well know n to those of skill in the art. See, e.g., Pearson, 1994, Methods Mol. Biol. 24:307-31 and 25:365-89.
  • mammal-produced milk refers to a milk produced by a mammal.
  • mammals include cow, human, sheep, mouflon, goat, buffalo, camel, horse, donkey, alpaca, yak, llama, lemur, panda, guinea pig, squirrel, bear, macaque, gorilla, chimpanzee, mountain goat, monkey, ape, cat, dog, wallaby, rat, mouse,
  • SUBSTITUTE SHEET (RULE 26) elephant, opossum, rabbit, whale, baboons, gibbons, orangutan, mandrill, pig, wolf, fox, lion, tiger, echidna, and woolly mammoth.
  • microbe or "microbial cell” as used herein is an abbreviation for microorganism, and includes all bacteria, archaea, unicellular protista, unicellular animals, unicellular plants, fungi (e.g., unicellular fungi, filamentous fungi, yeast), unicellular algae, protozoa, and chromista.
  • fungi e.g., unicellular fungi, filamentous fungi, yeast
  • microbial is used herein as the corresponding adjective.
  • milk protein as used herein refers to a whey protein or a casein.
  • milk protein component refers to a component that consists of a subset of whey proteins comprised in a mammal-produced milk, or of a subset of caseins comprised in a mammal-produced milk, or a mixture of a subset of whey proteins and a subset of caseins comprised in a mammal-produced milk, (i.e., consists of some but not all proteins present in, for example, whey protein concentrate, whey protein isolate, whey protein hydrolysate, casein isolate, casein concentrate, casein hydrolysate, milk, milk protein isolate, milk protein concentrate, milk protein hydrolysate, micellar casein concentrate, sodium caseinate, or acid caseinate).
  • the term implies that the milk proteins of which the milk protein component consists are the only milk proteins comprised in a composition provided herein (i.e., the composition comprises no other milk proteins other than the milk proteins of which the milk protein component consists).
  • non-native refers to what is found in nature in its unmodified state (e.g., a cell that is not genetically modified by a human, and that is maintained under conditions [e.g., level of oxygenation, pH, salt concentration, temperature, and nutrient (e.g., carbon, nitrogen, sulfur) availability] that are not defined by a human).
  • conditions e.g., level of oxygenation, pH, salt concentration, temperature, and nutrient (e.g., carbon, nitrogen, sulfur) availability
  • polynucleotide refers to a polymeric form of at least 2 (e.g., at least 5, at least 10, at least 20, at least 30, at least 40, at least 50, at least 100, at least 500, at least 1,000) nucleotides.
  • the term includes both sense and antisense strands of DNA molecules (e.g., cDNA, genomic DNA, synthetic DNA) and RNA molecules (e.g., mRNA, synthetic RNA), as well as analogs of DNA or RNA containing non-natural nucleotide analogs, non-native intemucleoside bonds, and/or chemical modifications.
  • a polynucleotide may be modified chemically or biochemically, or it may contain non-natural or derivatized nucleotide
  • SUBSTITUTE SHEET (RULE 26) bases.
  • modifications include, for example, labels; methylation; substitution of one or more of the naturally occurring nucleotides with an analog; intemucleotide modifications such as uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates), charged linkages (e.g., phosphorothioates, phosphorodithioates), pendent moieties (e.g., polypeptides), intercalators (e.g., acridine, psoralen), chelators, alkylators, and modified linkages (e.g., alpha anomeric nucleic acids).
  • uncharged linkages e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates
  • charged linkages e.g., phosphorothioates, phosphorodithioates
  • pendent moieties e.
  • modified nucleotides are described in the art (see, for example, Malyshev et al. 2014. Nature 509:385; Li et al. 2014. J. Am. Chem. Soc. 136:826). Also included are synthetic molecules that mimic polynucleotides in their ability to bind to a designated sequence via hydrogen bonding or other chemical interactions. Such molecules are known in the art and include, for example, molecules in which peptide linkages substitute for phosphate linkages in the backbone of the molecule. Other modifications can include, for example, analogs in which the ribose ring contains a bridging moiety or other structure, such as the modifications found in “locked” polynucleotides.
  • a polynucleotide can be in any topological conformation.
  • a polynucleotide can be single-stranded, double-stranded, triple-stranded, quadruplexed, partially double-stranded, branched, hairpinned, circular, or in a padlocked conformation.
  • polynucleotide sequence refers to a sequence of nucleotides that are comprised in a polynucleotide or of which a polynucleotide consists.
  • polypeptide and “protein” as used herein can be interchanged and refer to both a naturally occurring and a non-naturally occurring polymeric form of at least 2 (e.g., at least 5, at least 10, at least 20, at least 30, at least 40, at least 50, at least 100) amino acids, having an active structure or lacking a functional structure.
  • protein refers to a polypeptide of any length, which can include polypeptides comprising coded and non-coded amino acids, polypeptides compnsing amino acids that occur in nature, and those that do not occur in nature, polypeptides comprising chemically or biochemically modified or derivatized amino acids, and polypeptides comprising modified peptide backbones.
  • a protein can be monomeric, meaning it has a single chain, or polymeric, meaning it is composed of two or more chains, which can be covalently or non-covalently associated.
  • recombinant P-lactoglobulin refers to a recombinantly produced polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 50% (e.g., at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of amino acids in a native P-lactoglobulin (e.g., a native Bos
  • SUBSTITUTE SHEET (RULE 26) taurus P-lactoglobulin [e.g., amino acids 17 to 178 of UniProt sequence P02754], a native Ovis aries P-lactoglobulin [e.g., amino acids 19 to 180 of UniProt sequence P67976], a native Capra hircus P-lactoglobulin [e.g., amino acids 19 to 180 of UniProt sequence P02756], a native Equus caballus P-lactoglobulin [amino acids 19-180 of UniProt sequence P02758], a native Equus asinus P-lactoglobulin [UniProt sequence P13613], a native Equus caballus P- lactoglobulin [amino acids 19 to 181 of UniProt sequence P07380], a native Equus asinus
  • P-lactoglobulin e.g., amino acids 17 to
  • recombinant host cell refers to a host cell that comprises a recombinant polynucleotide.
  • a recombinant host cell may produce a polynucleotide or polypeptide not found in the native (non-recombinant) form of the host cell, or a recombinant host cell may produce a polynucleotide or polypeptide at a level that is different from that in the native (non-recombinant) form of the host cell. It should be understood that such term is intended to refer not only to the particular subject cell but also to the progeny of such a cell.
  • a recombinant host cell may be an isolated cell or cell line grown in culture or a cell that resides in a living tissue or organism.
  • recombinant milk protein refers to a milk protein, whey protein, casein, or non-milk protein, respectively, that is produced recombinantly (i.e., that is produced by a recombinant host cell).
  • the term "similar” as used herein refers to being within about +/— 15% with regard to a specified attribute.
  • the term includes being within about +/- 15%, about +/- 14%, about +/— 13%, about +/— 12%, about +/— 11%, about +/— 10%, about +/— 9%, about +/-8%, about +/-7%, about +/-6%, about +/— 5%, about +/-4%, about +/-3%, about +/-2%, or about +/-!% with regard to the specified attribute.
  • two or more refers to two, three, four, five, six, seven, eight, nine, ten, or more, or all of the elements subsequently listed.
  • hey protein refers to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical
  • SUBSTITUTE SHEET (RULE 26) to a sequence of amino acids in a whey protein natively found in a mammal-produced milk (i.e., a whey protein that is native to a mammal-produced milk; e.g., a native whey protein).
  • whey proteins include a-lactalbumin, [3-lactoglobulin, lactotransferrin, lactoferricin, serum albumin protein, lactoperoxidase protein, and glycomacropeptide.
  • a-lactalbumin refers to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of amino acids in an a-lactalbumin, P-lactoglobulin, lactotransferrin, lactoferricin, serum albumin, lactoperoxidase, and glyco
  • yeast refers to an organism of the order Saccharomycetales. Vegetative growth of yeast is by budding/blebbing of a unicellular thallus, and carbon catabolism may be fermentative.
  • % by mass refers to a percentage value for a mass as determined in a hydrated composition, such that the composition includes the mass of powder as well as the mass of the hydrating agent, with 100% fixed as the percentage value for the entire hydrated composition.
  • the term refers to a percentage value for a mass as determined relative to the eventual entire hydrated composition (with 100% fixed as the percentage value for that entire eventual hydrated composition).
  • a cosmetic or personal care composition that comprises a milk protein component, wherein the milk protein component comprises or consists essentially of a recombinant milk protein.
  • the cosmetic or personal care composition may comprise between 0.1% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3%, or 0.2%; between 0.2% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, or 0.3%; between 0.3% and 100%, 95%, 90%, 85%, 80%, 75%, 70%
  • SUBSTITUTE SHEET (RULE 26) 0.9%; between 0.9% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, or 1%; between 1% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, or 2%; between 2% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%,
  • SUBSTITUTE SHEET 50%; between 50% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, or 55%; between 55% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, or 60%; between 60% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, or 65%; between 65% and 100%, 95%, 90%, 85%, 80%, 75%, or 70%; between 70% and 100%, 95%, 90%, 85%, 80%, or 75%; between 75% and 100%, 95%, 90%, 85%, or 80%; between 80% and 100%, 95%, 90%, or 85%; or between 85% and 100%, 95%, 90%; between 90% and 100% or 95%, or between 95% and 100% by mass of the milk protein component.
  • the cosmetic or personal care composition according to any of the above may be in any form normally used for topical application, including a liquid (e.g., an aqueous dispersion, an oily dispersion, a dispersion in a solvent, an aqueous suspension, an aqueous-alcoholic solution, an oily solution, a milky liquid, a foaming liquid), an emulsion (e.g., water-in-oil emulsion, oil- in-water emulsion, water-in-oil-in-water emulsion, oil-in-water-in-oil emulsion, Pickering emulsion, microemulsion), a gel (e.g., a hydrogel, a lipogel, an organogel), a powder (e.g., an anhydrous powder or substantially anhydrous powder [e.g., pressed powder], a liquid
  • a liquid e.g., an aqueous dispersion, an oily dispersion,
  • the cosmetic or personal care composition according to any of the above when in powder form may comprise a moisture content of less than 20%, less than 15%, less than 10%, less than 7%, less than 5%, less than 3%, or less than 1%; or between 0.1% and 20%, 15%, 10%, 5%, or 1%; between 1% and 20%, 15%, 10%, or 5%; between 5% and 20%, 15%, or 10%; between 10% and 20%, or 15%; or between 15% and 20%.
  • the powder may be used in powder form, or may be reconstituted with a hydrating agent (e.g., water) prior to use, or may be mixed with other dry components before a hydrating agent is added to the mixture.
  • a hydrating agent e.g., water
  • the cosmetic or personal care composition according to any of the above when in emulsion form may comprise between 5% and 80%, 70%, 60%, 50%, 40%, 30%, 20%, or 10%; between 10% and 80%, 70%, 60%, 50%, 40%, 30%, or 20%; between 20% and 80%, 70%, 60%, 50%, 40%, or 30%; between 30% and 80%, 70%, 60%, 50%, or 40%; between 40% and 80%, 70%, 60%, or 50%; between 50% and 80%, 70%, or 60%; between 60% and 80%, or 70%; or between 70% and 80% by mass of lipid.
  • the cosmetic or personal care composition according to any of the above may further comprise an other ingredient (e.g., any of the other ingredients disclosed herein).
  • the cosmetic or personal care composition may comprise between 0.001% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%,
  • SUBSTITUTE SHEET (RULE 26) 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.1%, or 0.01%; between 0.01% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.1%; between 0.1% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, or 0.5%; between 0.5% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%,
  • the cosmetic or personal care composition according to any of the above may be essentially free of a component found in a mammal-produced milk, or may comprise a lower
  • SUBSTITUTE SHEET (RULE 26) concentration of at least one component found in a mammal-produced milk.
  • components found in mammal-produced milk include lactose, saturated fat, cholesterol, native milk proteins, and native milk lipids.
  • the cosmetic or personal care composition according to any of the above may be essentially free of a component obtained from an animal (i.e., a component that is native to an animal, including animal products [i.e., parts of an animal that are consumables or typically prepared for consumption by humans; e.g., animal meat, animal fat, animal blood], animal byproducts [i.e., products that are typically not consumable by themselves but are the byproducts of slaughtering animals for consumption; e g., animal bones, animal carcasses, and constituents isolated therefrom], products produced by an animal [e.g., mammal-produced milk, chicken eggs, bee honey], and consumables produced therefrom [e.g., gelatin, rennet, whey proteins extracted from mammal-produced milk, casein extracted from mammal- produced milk, milk lipid extracted from mammal-produced milk, animal lipids, animal proteins]), or comprise 2% or less by mass of such component.
  • an animal i.e., a component that is native to an
  • the cosmetic or personal care composition according to any of the above may be essentially free of antibiotics.
  • the cosmetic or personal care composition according to any of the above may be essentially free of animal hormones.
  • the cosmetic or personal care composition according to any of the above may be hypoallergenic.
  • the milk protein component comprised in a cosmetic or personal care composition according to any of the above comprises or consists essentially of a recombinant milk protein.
  • the recombinant milk protein may be a single recombinant milk protein, or may be a mixture of two or more recombinant milk proteins.
  • the milk protein component may, for example, comprise or consist essentially of: a recombinant P-lactoglobulin; a recombinant P- lactoglobulin and a recombinant a-lactalbumin; a recombinant P-lactoglobulin and a recombinant GMP; a recombinant P-lactoglobulin and a recombinant lactotransferrin; a recombinant P-lactoglobulin and a recombinant bovine serum albumin; a recombinant [L lactoglobulin and a recombinant lactoperoxidase; a recombinant [Tlactoglobulin and a recombinant K-casein; a recombinant P-lactoglobulin and a recombinant [3-casein; a recombinant P-lactoglobulin and a recombinant [3-case
  • SUBSTITUTE SHEET (RULE 26) and a recombinant GMP; a recombinant P-lactoglobulin, a recombinant a-lactalbumin. and a recombinant lactotransferrin; a recombinant P-lactoglobulin, a recombinant a-lactalbumin, and a recombinant bovine serum albumin; a recombinant P-lactoglobulin, a recombinant a- lactalbumm, and a recombinant lactoperoxidase; a recombinant P-lactoglobulin, a recombinant a-lactalbumin, and a recombinant K-casein; a recombinant P-lactoglobulin, a recombinant a- lactalbumin, and a recombinant P-casein; a recombinant P-lac
  • SUBSTITUTE SHEET (RULE 26) lactoglobulin, a recombinant 0-casein, and a recombinant a-S2-casein; a recombinant 0- lactoglobulin, a recombinant a-Sl -casein, and a recombinant a-S2-casein; a recombinant a- lactalbumm; a recombinant a-lactalbumin and a recombinant GMP; a recombinant a- lactalbumm and a recombinant lactotransferrin; a recombinant a-lactalbumin and a recombinant bovine serum albumin; a recombinant a-lactalbumin and a recombinant lactoperoxidase; a recombinant a-lactalbumin and a recombinant K-casein; a recombin
  • SUBSTITUTE SHEET (RULE 26) recombinant a-lactalbumin, a recombinant P-casein, and a recombinant a-S2-casein; a recombinant a-lactalbumin, a recombinant a-Sl -casein, and a recombinant a-S2-casein; a recombinant GMP; a recombinant GMP and a recombinant lactotransferrin; a recombinant GMP and a recombinant bovine serum albumin; a recombinant GMP and a recombinant lactoperoxidase; a recombinant GMP and a recombinant K-casein; a recombinant GMP and a recombinant P-casein; a recombinant GMP and a recombinant a-Sl -casein;
  • SUBSTITUTE SHEET (RULE 26) lactotransferrin, a recombinant bovine serum albumin, and a recombinant a-Sl -casein; a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant a-S2- casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant K- casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant P- casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant a- S1 -casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and
  • SUBSTITUTE SHEET (RULE 26) recombinant lactoperoxidase, a recombinant 0-casein, and a recombinant a-S2-casein; a recombinant lactoperoxidase, a recombinant a-Sl-casein, and a recombinant a-S2-casein; a recombinant K-casein; a recombinant K-casem and a recombinant -casein; a recombinant K- casein and a recombinant a-Sl -casein; a recombinant K-casein and a recombinant a-S2-casein; a recombinant K-casein, a recombinant
  • Recombinant milk proteins, and methods for producing recombinant milk proteins are disclosed, for example, in U.S. Patent 9,924,728, issued March 27, 2018; U.S. publication US20190216106, published July 18, 2019; and PCT publication WO2020219596, published October 29, 2020.
  • the milk protein component comprised in a cosmetic or personal care composition may comprise or consist of a multimer (e g., dimer, trimer, tetramer) or polymer of recombinant milk protein monomers (i.e., molecules composed of repeated recombinant milk protein monomers that are covalently linked, either directly with each other or via intermediary molecules).
  • the polymer of recombinant milk protein monomers may have the capacity to form a flexible film upon application of the cosmetic or personal care composition to a body surface.
  • the milk protein component comprised in a cosmetic or personal care composition may consist of whey protein and casein at a mass ratio of between about 10 to 0 and about 0 to 10 (e.g., about 10 to 0, about 10 to 1, about 9 to 1, about 8 to 1, about 7 to 1, about 6 to 1, about 5 to 1, about 4 to 1, about 3 to 1, about 2 to 1, about 1 to 1, about 1 to 2, about 1 to 3, about 1 to 4, about 1 to 5, about 1 to 6, about 1 to 7, about 1 to 8, about 1 to 9, about 1 to 10, about 0 to 10).
  • about 10 to 0 and about 0 to 10 e.g., about 10 to 0, about 10 to 1, about 9 to 1, about 8 to 1, about 7 to 1, about 6 to 1, about 5 to 1, about 4 to 1, about 3 to 1, about 2 to 1, about 1 to 1, about 1 to 2, about 1 to 3, about 1 to 4, about 1 to 5, about 1 to 6, about 1 to 7, about 1 to 8, about 1 to 9, about 1 to 10, about 0 to 10).
  • the milk protein component comprised in a cosmetic or personal care composition may comprise or consist of recombinant [3-lactoglobuhn and recombinant a-lactalbumin at a mass ratio of between about 10 to 0 and about 0 to 10 (e.g., about 10 to 0, about 10 to 1, about 9 to 1, about 8 to 1, about 7 to 1, about 6 to 1, about 5 to 1, about 4 to 1, about 3 to 1, about 2 to 1, about 1 to 1, about 1 to 2, about 1 to 3, about 1 to 4, about 1 to 5, about 1 to 6, about 1 to 7, about 1 to 8, about 1 to 9, about 1 to 10, about 0 to 10).
  • recombinant [3-lactoglobuhn and recombinant a-lactalbumin at a mass ratio of between about 10 to 0 and about 0 to 10 (e.g., about 10 to 0, about 10 to 1, about 9 to 1, about 8 to 1, about 7 to 1, about 6 to 1, about 5 to 1, about 4 to 1, about 3 to 1, about 2 to 1, about 1 to 1, about
  • the milk protein component comprised in a cosmetic or personal care composition according to any of the above may be comprised in a cosmetically or physiologically acceptable medium.
  • the milk protein component comprised in a cosmetic or personal care composition according to any of the above may be comprised in a coating layer around a core of the cosmetic or personal care composition.
  • Such core may comprise an active agent (e.g., any of the other ingredients disclosed herein).
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be undenatured (i.e., not heat-insolubilized as shown by electrophoretic measurements).
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be hydrolyzed (e.g., via action of an endopeptidase [e.g., trypsin, chymotrypsin]).
  • an endopeptidase e.g., trypsin, chymotrypsin
  • Such hydrolyzed recombinant milk protein may consist essentially of peptides having a molecular weight of between 200 to 5,000.
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be covalently or non- covalently associated with a lipid to form an amphiphilic complex.
  • suitable lipids for covalent association include fatty acids or acyls having carbon atom numbers of between 4 and 30, selected from fatty acids, fatty alcohols, and fatty amines, and derivatives thereof, and having chains that are unsaturated, linear, branched or cyclic.
  • Non-limiting examples include heptanoic acid, octanoic acid, decanoic acid, lauric acid, myristic acid, palmitic acid, stearic acid, ncinoleic acid, oleic acid, linoleic acid, linolenic acid, caprylic acid, capric acid; the corresponding fatty alcohols and fatty amines; the derivatives of said fatty acids, fatty alcohols, and fatty amines; and mixtures thereof.
  • the recombinant milk protein may comprise such lipid at a mass ratio of between about 1,000 to 1 and about 1 to 10.
  • the lipid may be covalently linked to the recombinant milk protein via a monofunctional agent (which may be the fatty containing component [fatty monoacid, monoalcohol, or monoamine]) or a multifunctional grafting agent (which may be the fatty containing component [fatty polyacid, polyalcohol, or poly amine]).
  • a monofunctional agent which may be the fatty containing component [fatty monoacid, monoalcohol, or monoamine]
  • a multifunctional grafting agent which may be the fatty containing component [fatty polyacid, polyalcohol, or poly amine]
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be covalently or non- covalently associated with a coloring agent (e.g., any of the coloring agents disclosed herein).
  • a coloring agent e.g., any of the coloring agents disclosed herein.
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be covalently or non- covalently associated with a UVA and/or UVB light filtering or UVA and/or UVB light scattering agent.
  • Non-limiting examples of UVA and/or UVB light filtering or UVA and/or UVB light scattering agents include BP-3 (2-hydroxy-4-methoxybenzophenone); cinnamoyl radical (optionally substituted by one or more alkoxy groups); p-dialkylaminobenzoyl radical; salicyloyl radical; sulphonyl radical originating from an isophthalylidene-camphor radical; acyl radical onginating from a carboxylic or sulphonic acid derived from benzylidenecamphor, 2-aiylbenzimidazoles, 2-arylbenzofurans, 2-arylbenzoxazoles, 2-arylbenzotriazoles, 2-aryhndoles, or hydroxybenzophenones; sulphonyl radical originating from a terephthalylidene-camphor radical; acyl radical derived from an absorber of carboxylic coumarin, monophenylcyanoacrylic, diphenylcyanoacryl
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be comprised in a coacervate with a polysaccharide.
  • the polysaccharide may be an anionic polysaccharide (i.e., a polysaccharide that has a negative total charge or linear charge density at a pH near the IEP of the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition), or a cationic polysaccharide (i.e., a polysacchande that has a positive total charge or linear charge density at the pH near the IEP of the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition).
  • Non-limiting examples of suitable anionic polysaccharides include polysaccharides having pendant carboxylic acid or sulfate groups, pectins (e.g., pectin from aloe, pectins from tobacco, pectins from apple pomace, pectins from citrus peel, pectins from plums, pectins from gooseberries sources, pectins from sugar beet), water soluble cellulose (e.g., sodium carboxymethyl cellulose, sulfated cellulose), seaweed hydrocolloid extracts (e.g., sodium alginate, propyleneglycol alginate, ammonium alginate, alginic acid, carageenans (e.g., iota carageenan, kappa carageenan, lambda carageenan)), microbial and agaroid gums and gum components (e.g., gellan gum, xanthan gum, gum Arabic, gum tragacanth
  • SUBSTITUTE SHEET (RULE 26) di ethylaminoethyl dextran, guar gum derivatives (e.g., guar hydroxypropyltrimonium chloride), quaternary nitrogen-containing cellulose ethers, copolymers of etherified cellulose, guar and starch, cationic cellulose, modified cationic cellulose polymers (e.g., polyquatemium 4, polyquatemium 10, polyquatemium 24, polyquatemium 28), laurdimonium hydroxypropyl oxyethyl cellulose, steardimonium hydroxyethyl cellulose, polysaccharides that comprise an amino group or an imine group, and derivatives thereof.
  • guar gum derivatives e.g., guar hydroxypropyltrimonium chloride
  • quaternary nitrogen-containing cellulose ethers e.g., guar hydroxypropyltrimonium chloride
  • the recombinant milk protein may be comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above as a gel (e.g., a hydrogel).
  • a gel e.g., a hydrogel
  • the other ingredient that may be comprised in a cosmetic or personal care composition according to any of the above may be any known ingredient normally used in cosmetic or personal care compositions.
  • the other ingredient should be of pharmaceutical or cosmetic grade, non-irritating on body surfaces, and non-toxic as used.
  • Non-limiting examples of other ingredients include: an aqueous solvent; a lipid; a wax; an emulsifying agent; a pH and/or ionic strength adjusting agent (i.e., a compound that can raise or lower the pH and/or the ionic strength of a solution; e.g., to balance the pH of the cosmetic or personal care composition to a pH of between 5 and 7); a buffering agent; a binding agent; a thickening agent; a filler agent (i.e., a colorless or white particle of any shape that is insoluble in the medium of the composition); a plasticizing agent; an anti-foam agent (e.g., to decrease foaming during compounding and handling of the composition); a foam stabilizer; a surfactant (e.g., a cationic, anionic, non-ionic, amphoteric, or zwitterionic surfactant); a gelling agent; a coloring agent (i.e., a molecule that
  • SUBSTITUTE SHEET (RULE 26) and/or scalp, for example by soothing the feelings of itching); a cooling agent (i.e. , a compound that provides a sensation of cooling); a fragrance; an anti-aging agent (i.e., a compound that inhibits or reduces signs of aging, such as wrinkles, fine lines, and manifestations of photodamage [e.g., sun spots]); an UVA and/or UVB light absorbing agent; an UVA and/or UVB light scattering agent; an antibacterial agent (i.e., a compound that has either a killing or growth inhibiting effect upon bacteria); an anti-fungal agent (i.e., a compound that has either a killing or growth inhibiting effect upon fungi); an anti-inflammatoiy agent (i.e., a compound effective in treating inflammation); a salt; a humectant; a hair growth agent; a hair anti-static agent; an anti-viral agent (i.e., a compound capable of
  • Non-limiting examples of aqueous solvents include water, floral water, mineral water, Cl to C6 alcohols (e.g., ethyl alcohol, propanol, isopropyl alcohol, butanol, pentanol, hexanol, glycerol), glycols (e.g., ethylene glycol, butylene glycol, isoprene glycol, propylene glycol, polyethylene glycol), glycol ethers (e.g., methyl ether, ethyl ether, butyl ether, 2- butoxyethanol, ethylene glycol monoethyl ether, propylene glycol, diethylene glycol monoethyl ether, di ethylene glycol monomethyl ether), and mixtures thereof.
  • alcohols e.g., ethyl alcohol, propanol, isopropyl alcohol, butanol, pentanol, hexanol, glycerol
  • glycols e.
  • Non-limiting examples of lipids include fatty alcohols (e.g., linoleyl alcohol, linolenyl alcohol, oleyl alcohol), polyalcohols (e.g., glycol, polyethylene glycol, polypropylene glycol, glycerol), fatty acid esters (e.g., alkyl myristates [e.g., isopropyl myristate, butyl myristate, cetyl myristate], alkyl stearates [e.g., butyl stearate, hexadecyl stearate], alkyl palmitates [e.g., ethyl palmitate, isopropyl palmitate], alkyl lanolates [e.g., isopropyl lanolate, isocetyl lanolate], alkyl oleates [e.g., decyl oleate], alkyl oct
  • Non-limiting examples of waxes include ozokerite wax, montan wax; ouricury wax,
  • Japan wax, cork fibre wax, sugar cane wax, lanolins e.g., lanolin, lanolin alcohol,
  • SUBSTITUTE SHEET (RULE 26) hydrogenated lanolin, acetylated lanolin, lanolin wax, lanolin fatty acids), beeswax, candellila wax, microcrystalline wax, carnauba wax, cetyl alcohol, stearyl alcohol, cocoa butter; paraffins, ozokerites, petrolatum, vaselines, petroleum jelly, hydrogenated oils that are solid at 25C, mono-/di-/tri-glycerides that are solid at 25C, fatty esters that are solid at 25C, silicone waxes (e.g., methyloctadecane-oxypolysiloxane, poly(dimethyl-siloxy) stearoxysiloxane), fluoro waxes, stearic acid monoethanolamide, colophony and its derivatives (e g., glycol abietate, glycerol abietate), hydrogenated oils that are solid at 25C, jojoba oil, sucroglycerides
  • Non-limiting examples of emulsifying agents include polyglycerin esters, sorbitan esters, partially esterified glycerides, and derivatives and mixtures thereof.
  • Non-limiting examples of pH adjusting agents include organic acids, mono- or triethanolamine, ammonia, sodium phosphate, sodium carbonate, phosphoric acid, hydrochloric acid, lactic acid, tartaric acid, acetic acid, citric acid, and mixtures thereof.
  • Non-limiting examples of buffering agents include ethylenediaminetetracetic acid (EDTA), sodium salt of EDTA, sodium chloride, sodium phosphate, diazolidinyl urea, iodopropynyl butylcarbamate, and mixtures thereof.
  • EDTA ethylenediaminetetracetic acid
  • sodium salt of EDTA sodium chloride
  • sodium phosphate sodium phosphate
  • diazolidinyl urea iodopropynyl butylcarbamate
  • Non-limiting examples of binding agents include silicone resins (e.g., silanes, siloxanes [e.g., polydimethylsiloxane (PDMS)], siloxysilicates [e.g., tnmethylsiloxysilicate], silsesqui oxanes [e.g., polymethylsilsesqui oxane]), dimethicone silylate/isododecane, polyglyceryyl-2 triisostearate, dry binders (e.g., zinc, lithium, magnesium stearate), and derivatives and mixtures thereof.
  • silicone resins e.g., silanes, siloxanes [e.g., polydimethylsiloxane (PDMS)], siloxysilicates [e.g., tnmethylsiloxysilicate], silsesqui oxanes [e.g., polymethylsilsesqui oxane]
  • Non-limiting examples of thickeners include salts, PEG-55, propylene glycol oleate, PEG- 120 methyl lucose ioleate, cellulose derivatives [e.g., carboxymethyl cellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose], and derivatives and mixtures thereof.
  • Non-limiting examples of fdlers include silica microspheres (e.g., hollow silica microspheres, acrylic polymer microspheres [e.g., microspheres made of crosslinked acrylate copolymer, microspheres made of polymethyl methacrylate]); polyurethane powders (e.g., powder of a copolymer of hexamethylene diisocyante and of trimethylol hexyl lactone); elastomeric crosslinked organopolysiloxane spherical powders; carnauba or paraffin wax microbeads; metal soaps in powder form (e.g., metal soaps of fatty acids containing from 12 to 22 carbons, where the metal may be zinc or magnesium, and the fatty acid may be lauric acid, myristic acid, stearic acid, and palamitic acid [e.g., zinc laurate, magnesium stearate, magnesium myristate, zinc stearate]), tal
  • SUBSTITUTE SHEET (RULE 26) aluminosilicates (e.g., micas of natural origin [e.g., muscovite, margarite, roscoelite, lepidolite, biotite] or of synthetic origin); clays (e.g., sericites); kaolin or hydrated aluminum silicate; boron nitrides; powders of tetrafluoroethylene polymers; precipitated calcium carbonate, magnesium carbonate, or magnesium hydrogen carbonate; hydroxyapatite; powders of nonexpanded synthetic polymers (e.g., polyethylene, polyesters [e.g., polyethylene isophthalate or terephthalate], polyamides [e.g., Nylon]); powders of spheronized, crosslinked or noncrosslinked synthetic polymers (e.g., polyamide powders [e.g., poly-P-alanine powder, Nylon powder, polyacrylic acid powder, polymethacrylic acid powder, powder
  • Non-limiting examples of plasticizing agents include glycerin, sorbitol, a non-toxic glycol or polyglycol (e.g., propylene dipropylene or other C2-C4 glycol), and derivatives and mixtures thereof.
  • Non-limiting examples of surfactants include alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkyl aryl sulfonates, alkyl succinates, alkyl sulfosuccinates, N-alkoyl sarcosinates, acyl taurates, acyl isothionates, alkyl phosphates, alkyl ether phosphates, alkyl ether carboxylates, alpha-olefin sulfonates (e.g., alkaline and earth alkaline metal salts [e.g., sodium, potassium, magnesium, calcium, ammonium, and triethanolamine salts]), alkyl betaines, alkyl amido propyle betaines, alkyl sulfobetaines, alkyl glycinates, alkyl carboxy glycinates, alkyl amphoacetates, alky prop
  • Non-limiting examples of coloring agents include dyes, lacquers, mineral pigments, organic pigments, nacres (i.e., colored particles of any form, which may or may not be iridescent, especially produced by certain molluses in their shell, or alternatively synthesized, and which have a color effect via optical interference), reflective particles (i.e., particles that impart sparkle), FD&C Blue No. 1 (Brilliant Blue), FD&C Blue No. 2 (Indigotine), FD&C Green No. 3 (Fast Green), FD&C Red No. 3 (Erythrosine), FD&C Red No. 40 (Allura Red), FD&C Yellow No. 5 (Tartrazine), FD&C Yellow No.
  • SUBSTITUTE SHEET (RULE 26) extract, copper-chlorophyll, copper-chlorophyllin, curcumin, curcumin/Cu-chlorophyllin, elderberry, grape, grape skin extract, hibiscus, lutein, mixed carotenoids, paprika, paprika extract, paprika oleoresin, riboflavin, saffron, spinach, stinging nettle, titanium dioxide, turmeric, titanium dioxide, zirconium oxide, cerium oxide, zinc oxide, iron (black, yellow or red) oxide, chromium oxide, sericites, micas, talcs, kaolin, manganese violet, ultramarine blue, chromium hydrate and ferric blue, metal powders (e.g., aluminum powder, copper powder), cochineal carmine, carotenoids, curcumin, porphyrin pigment, vegetable carbon black, organic pigments of azo dyes, anthraquinone dyes, indigoid
  • titanium mica coated with an iron oxide titanium mica coated with bismuth oxychloride, titanium mica coated with chromium oxide, pigments based on bismuth oxychloride, and particles comprising a borosilicate substrate coated with titanium oxide, and derivatives and mixtures thereof.
  • Non-limiting examples of agents that impart pearlescence include bismuth oxychloride, titanium mica, and guanine crystals.
  • Non-limiting examples of oil-dispersing agents include rhamnolipid biosurfactants, sophorolipid biosurfactants, polylipid protein sulfates, and derivatives and mixtures thereof.
  • conditioning agents include silicone compounds (e.g., polyalkyl siloxanes, polyaryl siloxanes, polyarylalkyl siloxanes, polyether siloxanes, silicone resins).
  • Non-limiting examples of anti-oxidants include alpha hydroxy acids (e.g., glycolic acid, citric acid, lactic acid, malic acid, mandelic acid, ascorbic acid, alpha-hydroxybutyric acid, alpha-hydroxyisobutyric acid, alpha-hydroxyisocaproic acid, atrrolactic acid, alphahydroxyisovaleric acid, ethyl pyruvate, galacturonic acid, glucopehtonic acid, glucopheptono 1,4-lactone, gluconic acid, gluconolactone, glucuronic acid, glucurronolactone, glycolic acid, isopropyl pyruvate, methyl pyruvate, mucic acid, pyruvia acid, saccharic acid, saccanc acid 1,4-lactone, tartaric acid, tartronic acid), chelating agents (e.g., metal chelators) such as ethylenediaminetetraacetic acid (EDTA), cit
  • SUBSTITUTE SHEET bis(o-aminophenoxy)ethane-N,N,N' ,N' -tetraacetic acid (BAPTA), diethylene triamine pentaacetic acid (DTP A), 2, 3-dimercapto-l -propanesulfonic acid (DMPS), dimercaptosuccinic acid (DMSA), a-lipoic acid, salicylaldehyde isonicotinoyl hydrazone (SIH), hexyl thioethylamine hydrochloride (HTA), desferrioxamine, ascorbic acid (vitamin C), cysteine, glutathione, dihydrolipoic acid, 2-mercaptoethane sulfonic acid, 2-mercaptobenzimidazole sulfonic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, sodium metabisulfite, vitamin E isomers (e.
  • BAPTA
  • Non-limiting examples of suitable anti-dandruff agents, anti-seborrheic dermatitis agents, and anti-psoriasis agents include zinc pyrithione, selenium sulfide, sulfur, sulfonated shale oil, salicylic acid, coal tar, povidone-iodine, imidazoles (e.g., ketoconazole, dichlorophenyl imidazolodioxalan, clotrimazole, itraconazoie, miconazole, climbazole, tioconazole, sulconazole, butoconazole, fluconazole, miconazolenitrite, anthralin), piroctone olamine (Octopirox), selenium sulfide, ciclopirox olamine, anti-psoriasis agents, vitamin A analogs, corticosteroids, and derivatives and mixtures thereof.
  • imidazoles e.g.,
  • Non-limiting examples of anti-acne agents include to keratolytics (e.g., salicylic acid, derivatives of salicylic acid, resorcinol); retinoids (e.g., retinoic acid, tretinoin, adapalene, tazarotene); sulfur-containing D- and L-amino acids and their derivatives and salts; lipoic acid; antibiotics and antimicrobials (e.g., lantibiotics [e.g., nisin, subtilin, epidermin, gallidermin], defensins, attacins [e.g., sarcotoxin], cecropins [e.g, cecropin A, bactericidin, lepidopteran], magainins, melittins, histatins, brevinins, novispirins, benzoyl peroxide, triclosan, chlorhexidine gluconate, octopi
  • Non-limiting examples of skin whitening agents include hydrogen peroxide, zinc peroxide, sodium peroxide, hydroquinone, 4-isopropylcatechol, hydroquinone monobenzyl ether, kojic acid, lactic acid, ascorbyl acid and derivatives (e.g., magnesium ascorbyl
  • SUBSTITUTE SHEET (RULE 26) phosphate), arbutin, licorice root, sugar amines (e.g., glucosamine, N-acetyl glucosamine, glucosamine sulfate, mannosamine, N-acetyl mannosamine, galactosamine, N-acetyl galactosamine), N-acyl amino acids (e.g., N-acyl phenylalanine, N-acyl tyrosine, N- undecylenoyl-L-phenylalanine), soy isoflavones, retinoids (e.g., retinol), kojic dipalmitate, arbutin, transexamic acid, vitamins (e.g., niacin, vitamin C), azelaic acid, linolenic acid, linoleic acid, placertia, natural extracts (e.g., chamomile, green tea
  • Non-limiting examples of skin self-tanning agents include dihydroxy acetone (DHA), glyceryl aldehyde, tyrosine and tyrosine derivatives (e.g., malyltyrosine, tyrosine glucosinate, ethyl tyrosine), phospho-DOPA, indoles, and derivatives and combinations thereof.
  • DHA dihydroxy acetone
  • tyrosine and tyrosine derivatives e.g., malyltyrosine, tyrosine glucosinate, ethyl tyrosine
  • phospho-DOPA indoles
  • Non-limiting examples of soothing agents include aloe, avocado oil, green tea extract, hops extract, chamomile extract, colloidal oatmeal, calamine, cucumber extract, sodium palmate, sodium palm kemelate, butyrospermum parkii (i.e., shea butter), menthe piperita (i.e., peppermint) leaf oil, sericin, pyridoxine (a form of vitamin B6), retinyl palmitate and/or other forms of vitamin A, tocopheryl acetate and/or other forms of vitamin E, lauryl laurate, hyaluronic acid, aloe barbadensis leaf juice powder, euterpe oleracea (i.e., acai berry) fruit extract, riboflavin (i.e., vitamin B2), thiamin HC1 and/or other forms of vitamin Bl, and variations and mixtures thereof.
  • menthe piperita i.e., peppermint
  • Non-limiting examples of cooling agents include WS-3, WS-23, menthol, 3- substituted-P-menthanes, N-substituted-P-menthane-3-carboxamides, isopulegol, 1,2- alkanediols (e.g., 3-(l-menthoxy)propane-l,2-diol, 3-(l-menthoxy)-2-methylpropane-l,2- diol, p-menthane-2,3-diol, p-menthane-3,8-diol), 6-isopropyl-9-methyl-l,4- dioxaspiro[4,5]decane-2-methanol, menthyl succinate and its alkaline earth metal salts, trimethylcyclohexanol, N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide, Japanese mint oil, peppermint oil, menthone, menthone glycerol
  • Non-limiting examples of fragrances include bisabolol; 2,4-dimethyl-3- cyclohexene-l-carbaldehyde; isocyclocitral; menthone; isomenthone; ROMASCONE® (methyl 2, 2-dimethyl-6-methylene-l -cyclohexanecarboxylate); nerone; terpineol; dihydroterpineol; terpenyl acetate; dihydroterpenyl acetate; dipentene; eucalyptol; hexylate;
  • SUBSTITUTE SHEET (RULE 26) rose oxide; PERYCOROLLE® ((S)-l,8-p-menthadiene-7-ol); l-p-menthene-4-ol; (lRS,3RS,4SR)-3-p-mentanyl acetate; (lR,2S,4R)-4,6,6-trimethyl-bicyclo[3,l,l]heptan-2-ol; DOREMOX® (tetrahydro-4-methyl-2-phenyl-2H-pyran); cyclohexyl acetate; cyclanol acetate; Fructalate (1,4-cyclohexane di ethyldicarboxylate); KOUMALACTONE® ((3ARS,6SR,7ASR)-perhydro-3,6-dimethyl-benzo[B]furan-2-one); Natactone ((6R)- perhydro-3,6-dimethyl-benzo[B]furan-2-one
  • SUBSTITUTE SHEET (RULE 26) 3-(l,l-dimethyl-5-indanyl)propanal); Rhubofix® (3' ,4-dimethyl- tricyclo[6.2.1.0(2,7)]undec-4-ene-9-spiro-2 z -oxirane); 9/10-ethyldiene-3- oxatricyclo[6.2.1 ,0(2,7)]undecane; POLYWOOD® (perhydro-5,5,8A-tnmethyl-2- naphthalenyl acetate); octalynol; CETALOX® (dodecahydro-3a,6,6,9a-tetramethyl- naphtho[2, l-b]furan); tricyclo[5.2.1 ,0(2,6)]dec-3-en-8-yl acetate and tricyclo[5.2.1 ,0(2,6)]dec-
  • Non-limiting examples of anti-aging agents include vitamins (e.g., vitamin E, vitamin A, vitamin C (ascorbic acid), vitamin B, vitamin E acetate, vitamin C palmitate), hydroxy acids (e.g., alpha-hydroxy acids [e.g., glycolic acid, lactic acid, 2-hydroxy butanoic acid, malic acid, citric acid tartaric acid, alpha-hydroxyethanoic acid, hydroxycaprylic acid], beta hydroxy acids [e.g., beta-hydroxybutyric acid, beta-phenyl-lactic acid, beta-phenylpyruvic acid, salicylic acid], polyhydroxy acids [e.g., gluconolactone (G4)]), gamma-linolenic acid, flavonoids (e.g., quercetin, hesperidin, quercitrin, rutin, tangeritin, epicatechin), CoQlO, retinoids, other proteins (e.g., collagen, soybean protein) and protein
  • UVA and/or UVB light absorbing agents include UV absorbers of benzoic acid system (e.g., para-aminobenzoic acid (PABA); PABA monoglycerin ester; N,N-dipropoxy PABA ethyl ester; N,N-diethoxy PABA ethyl ester; N,N-dimethyl PABA ethyl ester; N,N-dimethyl PABA butyl ester; N,N-dimethyl PABA methyl ester); UV absorbers of benzoic acid system (e.g., para-aminobenzoic acid (PABA); PABA monoglycerin ester; N,N-dipropoxy PABA ethyl ester; N,N-diethoxy PABA ethyl ester; N,N-dimethyl PABA ethyl ester; N,N-dimethyl PABA butyl ester; N,N-dimethyl PABA methyl ester); UV absorbers of
  • SUBSTITUTE SHEET (RULE 26) absorbers of anthranilic acid system (e.g., homomenthyl-N-acetyl anthranilate); UV absorbers of salicylic acid system (e.g., amyl salicylate; menthyl salicylate; homomenthyl salicylate; octyl salicylate; phenyl salicylate; benzyl salicylate; p-isopropanol phenyl salicylate); UV absorbers of cinnamic acid system (e.g., octyl cinnamate; ethyl-4-isopropyl cinnamate; methyl- 2,5-diisopropyl cinnamate; ethyl-2,4-diisopropyl cinnamate; methyl-2,4-diisopropyl cinnamate; propyl-p-methoxy cinnamate; isoprop
  • UVA and/or UVB light scattering agents include titanium oxide, particulate titanium oxide, zinc oxide, particulate zinc oxide, ferric oxide, particulate ferric oxide, ceric oxide, and derivatives and combinations thereof.
  • Non-limiting examples of antibacterial agents include macrolides or ketolides (e.g., erythromycin, azithromycin, clarithromycin, telithromycin); beta-lactams (e.g., penicillin, cephalosporin, carbapenems [e.g., carbapenem, imipenem, meropenem]); monolactams (e.g., penicillin G, penicillin V, methicillin, oxacillin, cioxacillin, dicloxacillin, nafcillin, ampicillin, amoxicillin, carbenicillin, ticarcillin, meziocillin, piperacillin, azlocillin, temocillin, cepalothin, cephapirin, cephradine, cephaloridine, cefazolin, cefamandole, cefuroxime, cephalexin, cefprozil, cefaclor, loracarbef,
  • SUBSTITUTE SHEET (RULE 26) sulphanilamides (e.g., para-aminobenzoic acid, sulfadiazine, sulfisoxazole, sulfamethoxazole, sulfathalidine); aminoglycosides (e.g., streptomycin, neomycin, kanamycin, paromycin, gentamicin, tobramycin, amikacin, netilmicin, spectinomycin, sisomicin, dibekalin, isepamicin); tetracyclines (e.g., tetracycline, chlortetracycline, demeclocy cline, minocycline, oxy tetracycline, methacycline, doxycycline); rifamycins (e.g., rifampicin [i.e., rifampin], rifapentine, rifabutin, bez
  • Non-limiting examples of antifungal agents include azoles (e.g., Fluconazole, Isavuconazole, Itraconazole, Ketoconazole (or a pyrithione salt thereof [e.g., zinc pyrithione, sodium pyrithione, potassium pyrithione, lithium pyrithione, ammonium pyrithione, copper pyrithione, calcium pyrithione, magnesium pyrithione, strontium pyrithione, silver pyrithione, gold pyrithione, manganese pyrithione, ethanolamine salt, chitosan salt, disulfide salt of pyrithione]), Miconazole, Clortrimazole, Voriconazole, Posaconazole, Ravuconazole, Ciclopirox, etc.), polyenes (e.g., natamycin, lucensomycin, nystatin, amphotericin B,
  • Non-limiting examples of anti-inflammatory agents include steroidal antiinflammatory (e.g., 21-acetoxypregnenolone, alclometasone, algestone, amcinonide, beclomethasone, betamethasone, budesonide, chloroprednisone, clobetasol, clobetansone, clocortolone, cloprednol, corticosterone, cortisone, cortivazol, deflazacort, desonide, desoximetasone, dexamethasone, diflorasone, diflucortolone, difluprednate, enoxolone, fluazacort, flucloronide, flumethasone flunisolide, fluocinolone acetonide, fluocinonide, fluocortin butyl, fluocortolone, fluoromethoIone, fluperolone acetate, fluprednidene a
  • SUBSTITUTE SHEET (RULE 26) or COX nonspecific inhibitors; e.g., salicylic acid derivatives (e.g., aspirin, sodium salicylate, choline magnesium trisalicylate, salicylate, diflunisal, sulfasalazine, olsalazine), paraaminophenol derivatives (e.g., acetaminophen); indole and indene acetic acids (e.g., indomethacin, sulindac); heteroaryl acetic acids (e.g, tolmetin, diclofenac, ketorolac); arylpropionic acids (e.g., ibuprofen, naproxen, flurbiprofen, ketoprofen, fenoprofen, oxaprozin); anthranilic acids (fenamates; e.g., mefenamic acid, meloxicam); enolic acids (
  • a cosmetic or personal care composition according to any of the above may have an attribute (e.g., any one of the attributes disclosed herein, or combination of two or more of the attributes disclosed herein) that is between 50% and 150%, 140%, 130%, 120%, 110%, 100%, 90%, 80%, 70%, or 60%; between 60% and 150%, 140%, 130%, 120%, 110%, 100%, 90%, 80%, or 70%; between 70% and 150%, 140%, 130%, 120%, 110%, 100%, 90%, or 80%; between 80% and 150%, 140%, 130%, 120%, 110%, 100%, or 90%; between 90% and 150%, 140%, 130%, 120%, 110%, or 100%; between 100% and 150%, 140%, 130%, 120%, or 110%; between 110% and 150%, 140%, 130%, 120%, or 110%; between 110% and 150%, 140%, 130%, or 120%; between 120% and 150%, 140%, or 130%; between 130% and 150%, or 14
  • Non-limiting examples of attributes include film forming (e.g., for even spreading on a body surface); film elasticity (e.g., elasticity of film formed on body surface); film flexibility (e.g., flexibility of film formed on body surface); adhesion to body surface (e.g., binding to sebum secreted from skin or keratin in hair to influence duration of wear); foam forming (e.g., foaming of hair shampoo); gelling; moisturizing of body surface; emulsifying (e.g., to stabilize oil droplets comprised in the cosmetic or personal care composition and/or capture oil secreted by skin to prevent pooling of oil); binding (e.g., water binding, lipid binding, binding of bioactive agent); delivery (e.g., delivery of bioactive compound); oilcontrolling and/or oil removing (e.g., absorption or breaking up into droplets of excess oil
  • SUBSTITUTE SHEET (RULE 26) produced and/or accumulated on body surface [e.g., to provide matte finish; to prevent acne, oily hair, or oily nail]); hypoallergenicity; softening of body surface; restructuring of body surface; glossing of body surface; de-glossing of body surface (e.g., to remove reflective shine); soothing of body surface (e.g., soothing of bum or sunbum); penetration of body surface (e.g., penetration into skin epidermis); revitalizing of body surface (e.g., modulate in situ production of collagen); activity on cutaneous necrosis; modulate (e.g., increase) rupture force of cicatrices; detangling of hair; strengthening, mending, and/or thickening of hair; humidity shielding (e.g., to enable long, sweat-free, and transfer-proof wear on body surface); encapsulating (e.g., to protect) active agent (e.g., any of the other ingredients disclosed herein); delivering active agent (e
  • the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care composition according to any of the above may impart or materially contribute to such attribute of the cosmetic or personal care composition.
  • the properties of milk proteins are in large part determined by the side chains of amino acids in the primary sequence of the milk proteins, the secondary and tertiary structures of the milk proteins, and on the interactions the secondary and tertiary structures permit the side chains to have with other molecules (e.g., other milk proteins, other non-milk proteins).
  • the primary sequence of milk proteins can be maintained in their recombinant production, amino acid side chains as well as secondary and tertiary structures may be impacted by protein folding pathways and non-native post-translational modifications (PTMs) that may occur in recombinant host cells.
  • PTMs post-translational modifications
  • SUBSTITUTE SHEET (RULE 26) of any of the above may have a non-native PTM that impacts a functionality of the recombinant milk protein, such that the recombinant milk protein imparts or materially contributes to an attribute of the cosmetic or personal care composition according to any of the above that differs from that imparted by a corresponding native milk protein in a corresponding cosmetic or personal care composition.
  • use of a native milk protein in production of a cosmetic or personal care composition often entails use of milk protein preparations that typically comprise at least small amounts of other components present in milk (e.g., other proteins, carbohydrates, minerals, lipids), which may impact an attribute of the cosmetic or personal care composition that is imparted or materially contributed to by the native milk protein.
  • recombinant production of a milk protein provides for preparations that are substantially purer, and that may therefor impart or materially contribute to an attribute of a cosmetic or personal care composition according to any of the above in a measurably different manner.
  • a cosmetic or personal care composition according to any of the above may comprise a lower amount of the recombinant milk protein than is necessary for the corresponding native protein in the corresponding cosmetic or personal care composition to obtain a similar attribute that is imparted or materially contributed to by the milk protein; and/or a cosmetic or personal care composition according to any of the above may comprise a different salt content than the corresponding cosmetic or personal care composition without effect on an attribute that is imparted or materially contributed to by the milk protein (e.g., without the recombinant milk protein becoming insoluble and leaving visible residue upon application of the cosmetic or personal care product on a body surface); and/or a cosmetic or personal care composition according to any of the above may have improved oil-controlling and/or oil removing capacity compared to the corresponding
  • a method for producing a cosmetic or personal care composition according to any of the above, wherein the method comprises the step of obtaining a recombinant milk protein.
  • the recombinant milk protein may be obtained using in vitro methods (e.g., using cell-free transcription and/or translation systems). Alternatively, the recombinant milk protein may be produced using a recombinant host cell.
  • the recombinant host cell may be derived from any wildtype organism, including any bacterium, fungus (e.g., yeast, filamentous fungus), archaea, unicellular protista, animal, plant, algae, protozoan, and chromista, or from a genetic variant (e.g., mutant) thereof, as well as from any generally recognized as safe (GRAS) industrial host cell.
  • Non-limiting examples of suitable bacteria include firmicutes, cyanobacteria (bluegreen algae), oscillatoriophcideae, bacillales, lactobacillales, oscillatoriales, bacillaceae, lactobacillaceae, and members of any of the following genera, and derivatives and crosses thereof: Acinetobacter, Acetobacter (e.g., Acetobacter suboxydans, Acetobacter xylinum), Actinoplane (e.g., Actinoplane missouriensis), Arthrospira (e.g., Arthrospira platensis, Arthrospira maxima), Bacillus (e g., Bacillus cereus, Bacillus coagulans, Bacillus licheniformis, Bacillus stearothermophilus, Bacillus subtilis), Escherichia (e.g., Escherichia coli), Lactobacillus (e.g., Lactobacillus acidophilus, Lac
  • Streptococcus thermophilus Streptomyces (e.g., Streptomyces chattanoogensis, Streptomyces gnseus, Streptomyces natalensis, Streptomyces olivaceus, Streptomyces olivochromogenes, Streptomyces rubiginosus), Tetrahymena (e.g., Tetrahymena thermophile, Tetrahymena hegewischi, Tetrahymena hyperangularis, Tetrahymena malaccensis, Tetrahymena pigmentosa, Tetrahymena pyriformis, Tetrahymena vorax ), and Xanthomonas (e.g., Xanthomonas campestns).
  • Streptomyces e.g., Streptomyces chattanoogensis, Streptomyces gnseus, Streptomyces natal
  • Non-limiting examples of suitable yeast include members of any of the following genera, and derivatives and crosses thereof: Blastobotrys, Candida (e g., Candida albicans, Candida etchellsii, Candida guilliermondii, Candida humilis, Candida lipolytica, Candida orthopsilosis, Candida palmioleophila, Candida pseudotropicalis, Candida sp., Candida utilis,
  • Candida e g., Candida albicans, Candida etchellsii, Candida guilliermondii, Candida humilis, Candida lipolytica, Candida orthopsilosis, Candida palmioleophila, Candida pseudotropicalis, Candida sp., Candida utilis,
  • SUBSTITUTE SHEET (RULE 26) Candida versatilis), Cladosporium, Cryptococcus (e.g., Cryptococcus terricolus, Cryptococcus curvatus), Debaryomyces (e.g., Debaryomyces hansenii), Endomyces (e.g., Endomyces vemalis), Endomycopsis (e.g., Endomycopsis vemalis), Eremothecium (e.g., Eremothecium ashbyii), Hansenula (e.g., Hansenula sp., Hansenula polymorpha), Kluyveromyces (e.g., Kluyveromyces sp., Kluyveromyces lactis, Kluyveromyces marxianus var.
  • Candida versatilis Cladosporium
  • Cryptococcus e.g., Cryptococcus terricolus, Cryptococcus curvat
  • Pichia e.g., Pichia sp., Pichia pastoris, Pichia finlandica, Pichia trehalophila, Pichia koclamae, Pichia membranaefaciens, Pichia minuta, Pichia lindnen), Pichia opuntiae, Pichia thermotolerans, Pichia salictaria, Pichia guercuum, Pichia pijperi, Pichia stiptis, Pichia methanolica), Rhodosporidium (e.g., Rhodosporidium toruloides), Rhodotorula (e.g., Rhodotorula s
  • Trichosporon cacaoliposimilis sp. nov. Trichosporon gracile, Trichosporon dulcitum, Trichosporon jirovecii, Trichosporon insectorum), Xanthophyllomyces (e.g., Xanthophyllomyces dendrorhous), Yarrowia (e.g., Yarrowia lipolytica), and Zygosaccharomyces (e.g., Zygosaccharomyces rouxii).
  • Xanthophyllomyces e.g., Xanthophyllomyces dendrorhous
  • Yarrowia e.g., Yarrowia lipolytica
  • Zygosaccharomyces e.g., Zygosaccharomyces rouxii).
  • Non-limiting examples of suitable filamentous fungi include any holomorphic, teleomorphic, and anamorphic forms of fungi, including members of any of the following genera, and derivatives and crosses thereof: Acremonium (e.g., Acremonium alabamense), Aspergillus (e.g., Aspergillus aculeatus, Aspergillus awamori, Aspergillus clavatus, Aspergillus flavus, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus niger var.
  • Acremonium e.g., Acremonium alabamense
  • Aspergillus e.g., Aspergillus aculeatus, Aspergillus awamori, Aspergillus clavatus, Aspergillus
  • Mucor e.g., Mucor miehei Cooney et Emerson (Rhizomucor miehei (Cooney & R. Emerson)
  • S chipper Mucor pusillus Lindt, Mucor circinelloides Mucor mucedo
  • Mycehophthora e.g., Myceliophthora thermophila
  • Myrothecium Neocallimastix
  • Neurospora e.g., Neurospora crassa
  • Paecilomyces Penicillium (e.g., Penicillium chrysogenum, Pennicillium iilacinum, Penicillium roquefortii), Phenerochaete, Phlebia, Piromyces, Pythium, Rhizopus (e.g., Rhizopus niveus), Schizophyllum, Scytalidium, Sporotrichum (e.g., Sporotrichum cellulophilum), Stere
  • Non-limiting examples of suitable plants include cycads, ginkgo biloba, conifers, cypress, junipers, thuja, cedarwood, pines, angelica, caraway, coriander, cumin, fennel, parsley, dill, dandelion, helichrysum, marigold, mugwort, safflower, camomile, lettuce,
  • SUBSTITUTE SHEET (RULE 26) wormwood, calendula, citronella, sages, thyme, chia seed, mustard, olive, coffee, capsicum, eggplant, paprika, cranberry, kiwi, vegetables (e.g., carrot, celery), tagetes, tansy, tarragon, sunflower, wintergreen, basil, hyssop, lavender, lemon verbena, marjoram, melissa, patchouli, pennyroyal, peppermint, rosemary, sesame, spearmint, primroses, samara, pepper, pimento, potato, sweet potato, tomato, blueberry, nightshades, petunia, morning glory, lilac, jasmin, honeysuckle, snapdragon, psyllium, wormseed, buckwheat, amaranth, chard, quinoa, spinach, rhubarb, jojoba, cypselea, chlor
  • the recombinant milk protein may be purified (i.e., be substantially separated from chemicals (e.g., carbohydrates, lipids, ash, metabolites, signaling molecules, other proteins), cellular components (e.g., cell walls, membrane lipids, chromosomes), and cells (e.g., other cells in an organism) of the source material from which the recombinant milk protein originated) comprised in a source material [e.g., a plant material, a fermentation broth]).
  • chemicals e.g., carbohydrates, lipids, ash, metabolites, signaling molecules, other proteins
  • cellular components e.g., cell walls, membrane lipids, chromosomes
  • cells e.g., other cells in an organism
  • the recombinant milk protein may be purified to a purity of greater than 30%, greater than 35%, greater than 40%, greater than 45%, greater than 50%, greater than 55%, greater
  • SUBSTITUTE SHEET (RULE 26) than 60%, greater than 65%, greater than 70%, greater than 75%, greater than 80%, greater than 85%, greater than 90%, greater than 95%, greater than 97%, or greater than 99% relative to other components comprised in the source material, or to at least 2-fold, at least 3 -fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, or at least 10-fold greater abundancy relative to other components comprised in the source material, or to a purity of greater than 30%, greater than 35%, greater than 40%, greater than 45%, greater than 50%, greater than 55%, greater than 60%, greater than 65%, greater than 70%, greater than 75%, greater than 80%, greater than 85%, greater than 90%, greater than 95%, greater than 97%, or greater than 99% by weight.
  • the method may further comprise the step of polymerizing the recombinant milk protein.
  • Methods for polymerizing milk proteins are disclosed, for example, in U.S. patent publication US20060057131, published March 16, 2006; PCT publication WO2019213155, published November 7, 2019; and PCT publication WO2022031941, published February 10, 2022.
  • the method may further comprise the step of producing a coacervate comprising the recombinant milk protein and a polysaccharide.
  • a coacervate comprising the recombinant milk protein and a polysaccharide.
  • a variety of recipes known in the art may be used to prepare cosmetic or personal care product.
  • the recombinant milk may be used in such recipes in purified/isolated form or comprised in a fermentation broth to produce a cosmetic or personal care product according to any of the above.
  • a method for beautifying or hygienizing comprising the step of applying to a body surface a cosmetic or personal care composition according to any of the above.
  • Example 1 Skin lightening activity of recombinant B-lactoglobulin in melanoderm skin model
  • the skin lightening activity of recombinantly produced B-lactoglobulin was evaluated using a MelanoDerm 24 tissue kit (MELB-300; MatTek, Ashland. MA).
  • MELB-300 MelanoDerm 24 tissue kit
  • EPI-100-NMM- 113 culture medium pre-warmed at 30-37C
  • the MEL-300B tissue was removed from packaging using sterile forceps and transferred into the 12 well hanging top plates.
  • the positive control exhibited the brightest skin (highest L* value).
  • Lower concentrations of recombinant B-lactoglobulin (0.63mg/ml, 0.2mg/ml, and 0.06mg/ml) showed higher L*values, indicating lighter skin.
  • concentrations (20mg/ml, 6.33mg/ml, and 2mg/ml)
  • the recombinant B-lactoglobulin showed lower L*values, suggesting less effective skin lightening.
  • Example 2 Anti-oxidant and anti-aging activity of recombinant B-lactoglobulin in epiderma skin model

Abstract

Provided are cosmetic or personal care compositions that comprise recombinant milk proteins, and methods for producing such cosmetic or personal care compositions.

Description

COSMETIC OR PERSONAL CARE COMPOSITION COMPRISING RECOMBINANT MILK PROTEIN
RELATED APPLICATIONS
[0001] This application claims priority to U.S. Provisional Patent Application Serial No. US63/391,511, filed on July 22, 2022, which is incorporated herein by reference, in its entirety.
FIELD OF THE INVENTION
[0002] The present invention relates generally to cosmetic or personal care compositions that comprise recombinant milk proteins. The present invention further provides methods for producing such cosmetic or personal care compositions.
BACKGROUND OF THE INVENTION
[0003] Many conventional cosmetic or personal care compositions comprise proteins derived from mammalian milk. These milk proteins impart or contribute to critical attributes and/or functionalities of such compositions.
[0004] There is, however, widespread concern over the impact of conventional milk production via animal husbandry on animal welfare and the environment. It is estimated that 30% of Earth's land surface is dedicated to animal farming, and that livestock account for 20% of the total terrestrial animal biomass. Due to its massive scale, animal farming produces more than 18% of net greenhouse gas emissions, constitutes the largest human source of water pollution, poses the world's largest threat to biodiversity, and in its industrialized implementation is cruel and exploitative.
[0005] Therefore, there exists a need for cosmetic or personal care products that, in their production and function, do not rely on the use of milk derived from animals, and for methods for producing such cosmetic or personal care products.
INCORPORATION BY REFERENCE
[0006] All publications, patents, patent applications, sequences, database entries, scientific publications, and other references mentioned herein are incorporated by reference in their entireties to the same extent as if each individual publication, patent, patent application, sequence, database entry, scientific publication, or other reference was specifically and individually indicated to be incorporated by reference. To the extent the material incorporated by reference contradicts or is inconsistent with the present disclosure, the present disclosure, including definitions, will supersede any such material.
1
SUBSTITUTE SHEET ( RULE 26) BRIEF SUMMARY OF THE INVENTION
[0007] In various aspects provided herein is a cosmetic or personal care composition comprising a milk protein component, wherein the milk protein component comprises or consists essentially of a recombinant milk protein.
[0008] The recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be a single recombinant milk protein. The single recombinant milk protein may be: a recombinant p-lactoglobulin, a recombinant a-lactalbumin, a recombinant GMP, a recombinant lactotransferrin, a recombinant bovine serum albumin, a recombinant lactoperoxidase, a recombinant K-casein, a recombinant -casein, a recombinant a-Sl -casein; or a recombinant a-S2-casein.
[0009] The recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be a mixture of two or more recombinant milk proteins. The mixture of two or more recombinant milk proteins may comprise: a recombinant P-lactoglobulin, a recombinant a-lactalbumin, a recombinant GMP, a recombinant lactotransferrin, a recombinant bovine serum albumin, a recombinant lactoperoxidase, a recombinant K-casein, a recombinant [3-casein, a recombinant a-Sl -casein; and/or a recombinant a-S2-casein.
[0010] The recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be hydrolyzed.
[0011] The recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition of paragraph [0007] may be a multimer or polymer of recombinant milk protein monomers.
[0012] In various further aspects provided herein is a method for producing a cosmetic or personal care composition of any of paragraphs [0007] through [0011], wherein the method comprises obtaining a recombinant milk protein. The recombinant milk protein may be obtained via production by a recombinant host cell. The recombinant host cell may be a recombinant fungal host cell (e.g., recombinant yeast host cell, recombinant filamentous fungal host cell). The recombinant fungal host cell may be derived from Saccharomyces, Kluyveromyces, Pichia, Tetrahymena, Yarrowia, Hansenula, Blastobotrys, Zygosaccharomyces, Debaryomyces, Aspergillus, Candida, Trichoderma, Fusarium, Chrysosporium, Physcomitrella, or Neurospora. The method may further comprise polymerizing the recombinant milk protein.
2
SUBSTITUTE SHEET ( RULE 26) [0013] In various further aspects provided herein is a method for beautifying or hygienizing, wherein the method comprises applying to a body surface a cosmetic or personal care composition of any of paragraphs [0007] through [0011], DETAILED DESCRIPTION OF THE INVENTION
[0014] The terminology and description used herein are for the purpose of describing particular embodiments only, and are not intended to limit the scope of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which this disclosure pertains. Further, unless otherwise required by context, singular terms shall include the plural, and plural terms shall include the singular.
Definitions
[0015] The terms "a" and "an" and "the" and similar references as used herein refer to both the singular and the plural (e.g., meaning "at least one" or "one or more"), unless otherwise indicated herein or clearly contradicted by context. For example, the term "a compound" is synonymous with the terms "at least one compound" and “one or more compounds”, and may refer to a single compound or to a plurality of compounds, including mixtures thereof.
[0016] The term "about" as used herein in conjunction with a stated numerical value or range of numerical values is meant to encompass variations of the stated numerical value or range of numerical values (i.e., denoting somewhat more or somewhat less than the stated numerical value or range of numerical values, to within a range of ±10% of the stated value or range of numerical values).
[0017] The term "and/or" as used herein refers to multiple components in combination with or exclusive of one another. For example, "x, y, and/or z" may refer to "x" alone, "y" alone, "z" alone, "x, y, and z", "(x and y) or z", "(x and z) or y", "(y and z) or x", "x and y" alone, "x and z" alone, "y and z" alone, or "x or y or z".
[0018] The terms “at least one” and “one or more” as used herein refer to one, two, three, four, five, six, seven, eight, nine, ten, at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, at least ten, or more, or all of the elements subsequently listed.
[0019] The term “body surface” as used herein refers to the exposed area of a human body. Non-limiting examples of body surfaces include skin, hair, nail, tooth, and tissues of the oral cavity (e.g., gums).
[0020] The term "casein" as used herein refers to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least
3
SUBSTITUTE SHEET ( RULE 26) 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of ammo acids in a casein natively found in a mammal-produced milk (i.e., a casein that is native to a mammal-produced milk; e.g., a native casein). Non-limiting examples of caseins include P-casein, /-casein, K-casein, a-Sl-casein, and a-S2-casein. Accordingly, the terms “P- casein”, “/-casein”, “K-casein”, “a-Sl -casein”, and “a-S2-casein” as used herein refer to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of amino acids in a P-casein, /-casein, K-casein, a- Sl-casein, and a-S2-casein, respectively, natively found in a mammal-produced milk (e.g., native P-casein (e.g., amino acids 16 to 224 of UniProt sequence P02666; amino acids 16 to 222 ofUniProt sequence Pl 1839 orP33048; amino acids 16 to 226 ofP05814), native /-casein, native K-casein (e.g., amino acids 22 to 190 of UniProt sequence P02668; amino acids 22 to 192 of UniProt sequence P02669 or P02670; amino acids 21 to 182 of UniProt sequence P07498), native a-Sl-casein (e.g., amino acids 16 to 214 ofUniProt sequence P02662, P04653, or P18626; amino acids 16 to 185 of UniProt sequence P47710), and native a-S2-casein (e.g., amino acids 16 to 222 ofUniProt sequence P02663; amino acids 16 to 223 ofUniProt sequence P04654 or P33049, respectively).
[0021] The term "corresponding cosmetic or personal care composition " as used herein refers to a cosmetic or personal care composition that is identical in composition to the cosmetic or personal care composition that is compared to the “corresponding cosmetic or personal care composition” except that the “corresponding cosmetic or personal care composition” comprises a corresponding native milk protein in place of the recombinant milk protein that is comprised in the cosmetic or personal care composition that is compared to the “corresponding cosmetic or personal care composition”.
[0022] The term “corresponding native milk protein” as used herein refers to a native milk protein that is identical in amino acid sequence to the recombinant milk protein that is compared to the “corresponding native milk protein” but that is obtained from a mammal- produced milk.
[0023] The term “cosmetic or personal care composition” as used herein refers to a composition that upon application to a body surface confers a perceived or actual beautifying
4
SUBSTITUTE SHEET ( RULE 26) or hygienizing effect. Non-limiting examples of cosmetic or personal care compositions include anti-wrinkling treatments (i.e., compositions used for tensioning [e.g., smoothing out of skin, reducing wrinkles in skin, removing fine lines in skm]), anti-aging treatments (i.e., compositions used for removing signs of aging [e.g., wrinkles, fine lines, manifestations of photodamage (e.g., sun spots)]), sun protection (i.e., compositions used to protect against UV exposure), anti-bum treatments (i.e., compositions used for soothing bums [e.g., sunbums]), anti-acne treatments (i.e., compositions that are effective in the treatment of acne and/or the symptoms associated therewith), skin cleansers (i.e., compositions used for cleaning skin and/or skin pores [e.g., nose strips for pore cleaning]), anti-dandruff treatments (i.e., compositions used for reducing or eliminating dandruff), anti-body odor treatments (i.e., compositions used for reducing or eliminating body odor), self-tanning treatments (i.e., compositions used for darkening skm color), skin whitening treatments (i.e., compositions used for bleaching/depigmenting skin color), hair coloring treatments (i.e., compositions used for coloring hair), lotions (e.g., skin lotions, body care lotions, wash lotions, moisture retention lotions, pre-shave lotions, after-shave lotions), pastes (e.g., washing pastes), ointments, balms, salves, masks, creams (e.g., water in oil creams, oil in water creams, day creams, night creams, eye creams, skin creams, face creams, anti-wrinkle creams, sun protection creams, moisture retention creams, after-shave creams, skin bleaching creams, self-tanning creams, vitamin creams, moisturizing creams, massage creams), eye care products (e.g., eye moisturizing mists, eye lens solutions), milks (e.g., body milks, cleansing milks), gels (e.g., anhydrous gels, shower gels), Eau de Toilette, soaps (e.g., transparent soaps, luxurious soaps, deodorant soaps, cream soaps, baby soaps, skin protection soaps, abrasive soaps, syndets, pasty soaps, soft soaps, peeling soaps), skin peeling treatments, liquid washes, shower and bath preparations (e.g., wash lotions, shower baths, shower gels, foam baths, oil baths, scrub preparations), foams (e.g., shaving foams, foam baths), deodorants, hair care products (e.g., shampoos, conditioners, hair mousses, hair colorants, hair sprays, rinse-off lotions, hair gels, hair emulsions, hair laquers, hair tonics, hair oils), lip glosses, sprays (e.g., hair sprays, pump sprays, sprays containing blowing agents), treatments for skin defects (e.g., dermatitis, weals, chaps, blemishes, cracks, scars, freckles, moles, rashes, blisters, pustules), toners, cleaning tissues, sanitary towels, tampons, nappies, repellents, make-up products (e.g., studio pigments, mascara, eye shadows, eyeliners, eye liner pens, rouges, face powders, eyebrow pencils, lipsticks, foundations, tinted creams, concealer sticks, blemish sticks, blushes, nail polish), sticks (e.g., lipsticks, concealer sticks, blemish sticks), hair removing agents, hand cleaning products, intimate hygiene products, foot care products, baby care products, and oral hygiene products (e.g., chewing
5
SUBSTITUTE SHEET ( RULE 26) gums, mouthwashes, toothpastes, gum-cleaning agents, denture adhesives, denture fixatives, breath strips, floss mechanisms, teeth whitening strips).
[0024] The term "essentially free of' as used herein refers to the indicated component being either not detectable in the indicated composition by common analytical methods, or to the indicated component being present in such trace amount as to not be functional. The term "functional" as used in this context refers to not materially contributing to properties of the composition comprising the trace amount of the indicated component, or to not having material activity (e.g., chemical activity, enzymatic activity) in the indicated composition comprising the trace amount of the indicated component, or to not having health-adverse effects upon use or consumption of the composition comprising the trace amount of the indicated component. The term “materially contributing” as used herein refers to the indicated component contributing to an attribute of a composition to such extent that in the absence of the component (e.g., in a reference composition that is identical to the composition except that it lacks the indicated component) the attribute is at least 10%, at least 20%, at least 30%, at least 40%, or at least 50% less present/active/measurable.
[0025] The term "filamentous fungus" as used herein refers to an organism from the filamentous form of the subdivision Eumycota and Oomycota (as defined by Hawksworth et al., In, Ainsworth and Bisby's Dictionary of The Fungi, 8th edition, 1995, CAB International, University Press, Cambridge, UK). A filamentous fungus is distinguished from a yeast by its hyphal elongation during vegetative growth.
[0026] The term "fungus" as used herein refers to organisms of the phyla Ascomycotas, Basidiomycota, Zygomycota, and Chythridiomycota, Oomycota, and Glomeromycota. It is understood, however, that fungal taxonomy is continually evolving, and therefore this specific definition of the fungal kingdom may be adjusted in the future.
[0027] The term "host cell" as used herein refers not only to the particular subject cell but also to the progeny of such cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the subject cell, but are still included within the scope of the term "host cell" as used herein.
[0028] The term “identity ” or “identical” in the context of two or more polynucleotide or polypeptide sequences as used herein refer to the nucleotide or amino acid residues that are the same when the two or more polynucleotide or polypeptide sequences, respectively, are aligned for maximum correspondence. Depending on the application, the "identity" may exist over a region of the sequences being compared (e.g., over the length of a functional domain) or over
6
SUBSTITUTE SHEET ( RULE 26) the full length of the sequences. A “region” is considered to be a continuous stretch of at least 6, 9, 14, 19, 24, 29, 34, 39, or more nucleotides, or of at least 2, 6, 10, 14, 18, 22, 26, 30, or more amino acids. For comparison, typically one sequence acts as a reference sequence to which one or more test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary', and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters. Optimal alignment of sequences for comparison may be conducted, e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Sequence Analysis Software Package of the Genetics Computer Group (GCG), University of Wisconsin Biotechnology Center, which may be used with default parameters), or by visual inspection (see generally Ausubel et al., infra). One example of an algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm (see, for example, Altschul et al. 1990 J. Mol. Biol. 215:403-410; Gish & States. 1993 Nature Genet. 3:266-272; Madden et al. 1996 Meth. Enzymol. 266:131-141; Altschul et al. 1997 Nucleic Acids Res. 25:3389-3402; Zhang 7 Madden. 1997 Genome Res. 7:649-656). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. In cases where two or more polypeptide sequences differ from each other by conservative substitutions (i.e., substitutions of amino acids with chemically similar amino acids; conservative substitution tables providing functionally similar amino acids are well known in the art), the percent sequence identity or degree of homology may be adjusted upwards to correct for the conservative nature of the substitution. Means for making this adjustment are well know n to those of skill in the art. See, e.g., Pearson, 1994, Methods Mol. Biol. 24:307-31 and 25:365-89.
[0029] The terms “including,” “includes,” “having,” “has,” “with,” or variants thereof as used herein are intended to be inclusive in a manner similar to the term “comprising”.
[0030] The term “mammal-produced milk” as used herein refers to a milk produced by a mammal. Non-limiting examples of mammals include cow, human, sheep, mouflon, goat, buffalo, camel, horse, donkey, alpaca, yak, llama, lemur, panda, guinea pig, squirrel, bear, macaque, gorilla, chimpanzee, mountain goat, monkey, ape, cat, dog, wallaby, rat, mouse,
7
SUBSTITUTE SHEET ( RULE 26) elephant, opossum, rabbit, whale, baboons, gibbons, orangutan, mandrill, pig, wolf, fox, lion, tiger, echidna, and woolly mammoth.
[0031] The term "microbe" or "microbial cell" as used herein is an abbreviation for microorganism, and includes all bacteria, archaea, unicellular protista, unicellular animals, unicellular plants, fungi (e.g., unicellular fungi, filamentous fungi, yeast), unicellular algae, protozoa, and chromista. The term "microbial" is used herein as the corresponding adjective. [0032] The term "milk protein" as used herein refers to a whey protein or a casein.
[0033] The term "milk protein component" as used herein refers to a component that consists of a subset of whey proteins comprised in a mammal-produced milk, or of a subset of caseins comprised in a mammal-produced milk, or a mixture of a subset of whey proteins and a subset of caseins comprised in a mammal-produced milk, (i.e., consists of some but not all proteins present in, for example, whey protein concentrate, whey protein isolate, whey protein hydrolysate, casein isolate, casein concentrate, casein hydrolysate, milk, milk protein isolate, milk protein concentrate, milk protein hydrolysate, micellar casein concentrate, sodium caseinate, or acid caseinate). The term implies that the milk proteins of which the milk protein component consists are the only milk proteins comprised in a composition provided herein (i.e., the composition comprises no other milk proteins other than the milk proteins of which the milk protein component consists).
[0034] The term "native" as used herein refers to what is found in nature in its unmodified state (e.g., a cell that is not genetically modified by a human, and that is maintained under conditions [e.g., level of oxygenation, pH, salt concentration, temperature, and nutrient (e.g., carbon, nitrogen, sulfur) availability] that are not defined by a human).
[0035] The terms "optional" or "optionally" as used herein refer to a feature or structure being present or not, or an event or circumstance occurring or not. The description includes instances in which a feature or structure is present, instances in which a feature or structure is absent, instances in which an event or circumstance occurs, and instances in which an event or circumstance does not occur.
[0036] The term “polynucleotide” as used herein refers to a polymeric form of at least 2 (e.g., at least 5, at least 10, at least 20, at least 30, at least 40, at least 50, at least 100, at least 500, at least 1,000) nucleotides. The term includes both sense and antisense strands of DNA molecules (e.g., cDNA, genomic DNA, synthetic DNA) and RNA molecules (e.g., mRNA, synthetic RNA), as well as analogs of DNA or RNA containing non-natural nucleotide analogs, non-native intemucleoside bonds, and/or chemical modifications. A polynucleotide may be modified chemically or biochemically, or it may contain non-natural or derivatized nucleotide
8
SUBSTITUTE SHEET ( RULE 26) bases. Such modifications include, for example, labels; methylation; substitution of one or more of the naturally occurring nucleotides with an analog; intemucleotide modifications such as uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates), charged linkages (e.g., phosphorothioates, phosphorodithioates), pendent moieties (e.g., polypeptides), intercalators (e.g., acridine, psoralen), chelators, alkylators, and modified linkages (e.g., alpha anomeric nucleic acids). Examples of modified nucleotides are described in the art (see, for example, Malyshev et al. 2014. Nature 509:385; Li et al. 2014. J. Am. Chem. Soc. 136:826). Also included are synthetic molecules that mimic polynucleotides in their ability to bind to a designated sequence via hydrogen bonding or other chemical interactions. Such molecules are known in the art and include, for example, molecules in which peptide linkages substitute for phosphate linkages in the backbone of the molecule. Other modifications can include, for example, analogs in which the ribose ring contains a bridging moiety or other structure, such as the modifications found in “locked” polynucleotides. A polynucleotide can be in any topological conformation. For instance, a polynucleotide can be single-stranded, double-stranded, triple-stranded, quadruplexed, partially double-stranded, branched, hairpinned, circular, or in a padlocked conformation. The term “polynucleotide sequence” as used herein refers to a sequence of nucleotides that are comprised in a polynucleotide or of which a polynucleotide consists.
[0037] The terms “polypeptide” and “protein” as used herein can be interchanged and refer to both a naturally occurring and a non-naturally occurring polymeric form of at least 2 (e.g., at least 5, at least 10, at least 20, at least 30, at least 40, at least 50, at least 100) amino acids, having an active structure or lacking a functional structure. The term “protein” as used herein refers to a polypeptide of any length, which can include polypeptides comprising coded and non-coded amino acids, polypeptides compnsing amino acids that occur in nature, and those that do not occur in nature, polypeptides comprising chemically or biochemically modified or derivatized amino acids, and polypeptides comprising modified peptide backbones. A protein can be monomeric, meaning it has a single chain, or polymeric, meaning it is composed of two or more chains, which can be covalently or non-covalently associated.
[0038] The term "recombinant P-lactoglobulin" as used herein refers to a recombinantly produced polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 50% (e.g., at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of amino acids in a native P-lactoglobulin (e.g., a native Bos
9
SUBSTITUTE SHEET ( RULE 26) taurus P-lactoglobulin [e.g., amino acids 17 to 178 of UniProt sequence P02754], a native Ovis aries P-lactoglobulin [e.g., amino acids 19 to 180 of UniProt sequence P67976], a native Capra hircus P-lactoglobulin [e.g., amino acids 19 to 180 of UniProt sequence P02756], a native Equus caballus P-lactoglobulin [amino acids 19-180 of UniProt sequence P02758], a native Equus asinus P-lactoglobulin [UniProt sequence P13613], a native Equus caballus P- lactoglobulin [amino acids 19 to 181 of UniProt sequence P07380], a native Equus asinus |3- lactoglobulin [UniProt sequence Pl 9647]).
[0039] The term "recombinant host cell" as used herein refers to a host cell that comprises a recombinant polynucleotide. Thus, for example, a recombinant host cell may produce a polynucleotide or polypeptide not found in the native (non-recombinant) form of the host cell, or a recombinant host cell may produce a polynucleotide or polypeptide at a level that is different from that in the native (non-recombinant) form of the host cell. It should be understood that such term is intended to refer not only to the particular subject cell but also to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not be identical to the subject cell, but are still included within the scope of the term "recombinant host cell" as used herein. A recombinant host cell may be an isolated cell or cell line grown in culture or a cell that resides in a living tissue or organism.
[0040] The term "recombinant milk protein", “recombinant whey protein”, “recombinant casein”, or “recombinant non-milk protein” as used herein refers to a milk protein, whey protein, casein, or non-milk protein, respectively, that is produced recombinantly (i.e., that is produced by a recombinant host cell).
[0041] The term "similar" as used herein refers to being within about +/— 15% with regard to a specified attribute. The term includes being within about +/- 15%, about +/- 14%, about +/— 13%, about +/— 12%, about +/— 11%, about +/— 10%, about +/— 9%, about +/-8%, about +/-7%, about +/-6%, about +/— 5%, about +/-4%, about +/-3%, about +/-2%, or about +/-!% with regard to the specified attribute.
[0042] The term "two or more” as used herein refers to two, three, four, five, six, seven, eight, nine, ten, or more, or all of the elements subsequently listed.
[0043] The term "whey protein" as used herein refers to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical
10
SUBSTITUTE SHEET ( RULE 26) to a sequence of amino acids in a whey protein natively found in a mammal-produced milk (i.e., a whey protein that is native to a mammal-produced milk; e.g., a native whey protein). Non-limitmg examples of whey proteins include a-lactalbumin, [3-lactoglobulin, lactotransferrin, lactoferricin, serum albumin protein, lactoperoxidase protein, and glycomacropeptide. Accordingly, the terms “a-lactalbumin”, “P-lactoglobulin”, “lactotransferrin”, “lactoferricin”, “serum albumin”, “lactoperoxidase”, and “glycomacropeptide” as used herein refer to a polypeptide that comprises a sequence of at least 20 (e.g., at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150) amino acids that is at least 40% (e.g., at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 99%, 100%) identical to a sequence of amino acids in an a-lactalbumin, P-lactoglobulin, lactotransferrin, lactoferricin, serum albumin, lactoperoxidase, and glycomacropeptide (GMP), respectively, natively found in a mammal- produced milk (e.g., native a-lactalbumin protein (e.g., amino acids 20-142 of UniProt sequence P00709, P00711, P00712, or P09462), native P-lactoglobulin protein (amino acids 17-178 of UniProt sequence P02754, amino acids 19-180 of UniProt sequence P67976 or P02756), native lactotransferrin protein (e.g., amino acids 20 to 708 of UniProt sequence P24627, D3G9G3, or Q29477; amino acids 20 to 710 of UniProt sequence P02788), native lactoferricin (amino acids 36 to 60 of UniProt sequence P24627), native serum albumin protein (e.g., amino acids 25 to 607 of UniProt sequence P02769 or P14639; amino acids 19 to 608 of UniProt sequence A0A452F7Y5; amino acids 25 to 609 of UniProt sequence P02768), native lactoperoxidase protein (amino acids 101 to 712 of UniProt sequence P80025), and native glycomacropeptide (GMP; e.g., amino acids 127 to 190 of UniProt sequence P02668)).
[0044] The term "yeast" as used herein refers to an organism of the order Saccharomycetales. Vegetative growth of yeast is by budding/blebbing of a unicellular thallus, and carbon catabolism may be fermentative.
[0045] The term “% by mass” as used herein refers to a percentage value for a mass as determined in a hydrated composition, such that the composition includes the mass of powder as well as the mass of the hydrating agent, with 100% fixed as the percentage value for the entire hydrated composition. In embodiments in which the composition provided herein is in powder form (to which the mass of the hydrating agent will be added at a later time), the term refers to a percentage value for a mass as determined relative to the eventual entire hydrated composition (with 100% fixed as the percentage value for that entire eventual hydrated composition).
11
SUBSTITUTE SHEET ( RULE 26) [0046] Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value (fractional or integral) falling within the range inclusive of the recited minimum and maximum value, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. Also, it should be understood that any numerical range recited herein is intended to include all sub-ranges subsumed therein.
[0047] It further should be understood that in any method disclosed herein the order of steps or order for performing certain actions is immaterial so long as the invention remains operable. Moreover, two or more steps or actions may be conducted simultaneously.
Animal-Free Cosmetic Or Personal Care Composition
[0048] In various aspects, provided herein is a cosmetic or personal care composition that comprises a milk protein component, wherein the milk protein component comprises or consists essentially of a recombinant milk protein.
[0049] The cosmetic or personal care composition may comprise between 0.1% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, 0.3%, or 0.2%; between 0.2% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, 0.4%, or 0.3%; between 0.3% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, 0.5%, or 0.4%; between 0.4% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6%, or 0.5%; between 0.5% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, or 0.6%; between 0.6% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, or 0.7%; between 0.7% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, or 0.8%; between 0.8% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, or
12
SUBSTITUTE SHEET ( RULE 26) 0.9%; between 0.9% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, or 1%; between 1% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, or 2%; between 2% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, or 3%; between 3% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, 5%, or 4%; between 4% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, 6%, or 5%; between 5% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, 7%, or 6%; between 6% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, or 7%; between 7% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, 9%, or 8%; between 8% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, 10%, or 9%; between 9% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, 11%, or 10%; between 10% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, 12%, or 11%; between 11% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, 13%, or 12%; between 12% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 14%, or 13%; between 13% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, or 14%; between 14% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, or 15%; between 15% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, or 20%; between 20% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, or 25%; between 25% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, or 30%; between 30% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, or 35%; between 35% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, or 40%; between 40% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, or 45%; between 45% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, or
13
SUBSTITUTE SHEET ( RULE 26) 50%; between 50% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, or 55%; between 55% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, or 60%; between 60% and 100%, 95%, 90%, 85%, 80%, 75%, 70%, or 65%; between 65% and 100%, 95%, 90%, 85%, 80%, 75%, or 70%; between 70% and 100%, 95%, 90%, 85%, 80%, or 75%; between 75% and 100%, 95%, 90%, 85%, or 80%; between 80% and 100%, 95%, 90%, or 85%; or between 85% and 100%, 95%, 90%; between 90% and 100% or 95%, or between 95% and 100% by mass of the milk protein component.
[0050] At standard ambient temperature and conditions (i.e., 20-30°C and 0.95-1.05 atm), the cosmetic or personal care composition according to any of the above may be in any form normally used for topical application, including a liquid (e.g., an aqueous dispersion, an oily dispersion, a dispersion in a solvent, an aqueous suspension, an aqueous-alcoholic solution, an oily solution, a milky liquid, a foaming liquid), an emulsion (e.g., water-in-oil emulsion, oil- in-water emulsion, water-in-oil-in-water emulsion, oil-in-water-in-oil emulsion, Pickering emulsion, microemulsion), a gel (e.g., a hydrogel, a lipogel, an organogel), a powder (e.g., an anhydrous powder or substantially anhydrous powder [e.g., pressed powder], a liquid-to- powder formulation, a cream-to-powder formulation), an aerosol, a semi-solid (e.g., a paste, a cream, an ointment, a lotion, a mousse), or a solid (e.g., a stick, a tube).
[0051] The cosmetic or personal care composition according to any of the above when in powder form may comprise a moisture content of less than 20%, less than 15%, less than 10%, less than 7%, less than 5%, less than 3%, or less than 1%; or between 0.1% and 20%, 15%, 10%, 5%, or 1%; between 1% and 20%, 15%, 10%, or 5%; between 5% and 20%, 15%, or 10%; between 10% and 20%, or 15%; or between 15% and 20%. The powder may be used in powder form, or may be reconstituted with a hydrating agent (e.g., water) prior to use, or may be mixed with other dry components before a hydrating agent is added to the mixture.
[0052] The cosmetic or personal care composition according to any of the above when in emulsion form may comprise between 5% and 80%, 70%, 60%, 50%, 40%, 30%, 20%, or 10%; between 10% and 80%, 70%, 60%, 50%, 40%, 30%, or 20%; between 20% and 80%, 70%, 60%, 50%, 40%, or 30%; between 30% and 80%, 70%, 60%, 50%, or 40%; between 40% and 80%, 70%, 60%, or 50%; between 50% and 80%, 70%, or 60%; between 60% and 80%, or 70%; or between 70% and 80% by mass of lipid.
[0053] The cosmetic or personal care composition according to any of the above may further comprise an other ingredient (e.g., any of the other ingredients disclosed herein). For example, the cosmetic or personal care composition may comprise between 0.001% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%,
14
SUBSTITUTE SHEET ( RULE 26) 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, 0.1%, or 0.01%; between 0.01% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.1%; between 0.1% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, or 0.5%; between 0.5% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, or 1%; between 1% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, or 2%; between 2% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, or 3%; between 3% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, or 4%; between 4% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, or 5%; between 5% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, or 6%; between 6% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, 8%, or 7%; between 7% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, 9%, or 8%; between 8% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, 10%, or 9%; between 9% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, 15%, or 10%; between 10% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, 20%, or 15%; between 15% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 25%, or 20%; between 20% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50% or 25%; between 25% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, or 50%; between 50% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, or 60%; between 60% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, or 70%; between 70% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, 90%, or 80%; between 80% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, 95%, or 90%; between 90% and 99.9%, 99.5%, 99%, 98%, 97%, 96%, or 95%; or between 95% and 99.9%, 99.5%, 99%, 98%, 97%, or 96%; between 96% or 99.9%, 99.5%, 99%, 98%, or 97%; between 97% and 99.9%, 99.5%, 99%, or 98%; between 98% and 99.9%, 99.5%, or 99%; between 99% and 99.9%, or 99.5%; or between 99.5% and 99.9% by mass of any one such other ingredient, any combination of two or more such other ingredients, or all such other ingredients.
[0054] The cosmetic or personal care composition according to any of the above may be essentially free of a component found in a mammal-produced milk, or may comprise a lower
15
SUBSTITUTE SHEET ( RULE 26) concentration of at least one component found in a mammal-produced milk. Non-limiting examples of components found in mammal-produced milk include lactose, saturated fat, cholesterol, native milk proteins, and native milk lipids.
[0055] The cosmetic or personal care composition according to any of the above may be essentially free of a component obtained from an animal (i.e., a component that is native to an animal, including animal products [i.e., parts of an animal that are consumables or typically prepared for consumption by humans; e.g., animal meat, animal fat, animal blood], animal byproducts [i.e., products that are typically not consumable by themselves but are the byproducts of slaughtering animals for consumption; e g., animal bones, animal carcasses, and constituents isolated therefrom], products produced by an animal [e.g., mammal-produced milk, chicken eggs, bee honey], and consumables produced therefrom [e.g., gelatin, rennet, whey proteins extracted from mammal-produced milk, casein extracted from mammal- produced milk, milk lipid extracted from mammal-produced milk, animal lipids, animal proteins]), or comprise 2% or less by mass of such component.
[0056] The cosmetic or personal care composition according to any of the above may be essentially free of antibiotics.
[0057] The cosmetic or personal care composition according to any of the above may be essentially free of animal hormones.
[0058] The cosmetic or personal care composition according to any of the above may be hypoallergenic.
Milk Protein Component
[0059] The milk protein component comprised in a cosmetic or personal care composition according to any of the above comprises or consists essentially of a recombinant milk protein. The recombinant milk protein may be a single recombinant milk protein, or may be a mixture of two or more recombinant milk proteins. The milk protein component may, for example, comprise or consist essentially of: a recombinant P-lactoglobulin; a recombinant P- lactoglobulin and a recombinant a-lactalbumin; a recombinant P-lactoglobulin and a recombinant GMP; a recombinant P-lactoglobulin and a recombinant lactotransferrin; a recombinant P-lactoglobulin and a recombinant bovine serum albumin; a recombinant [L lactoglobulin and a recombinant lactoperoxidase; a recombinant [Tlactoglobulin and a recombinant K-casein; a recombinant P-lactoglobulin and a recombinant [3-casein; a recombinant P-lactoglobulin and a recombinant a-Sl -casein; a recombinant P-lactoglobulin and a recombinant a-S2-casem; a recombinant P-lactoglobulin, a recombinant a-lactalbumin,
16
SUBSTITUTE SHEET ( RULE 26) and a recombinant GMP; a recombinant P-lactoglobulin, a recombinant a-lactalbumin. and a recombinant lactotransferrin; a recombinant P-lactoglobulin, a recombinant a-lactalbumin, and a recombinant bovine serum albumin; a recombinant P-lactoglobulin, a recombinant a- lactalbumm, and a recombinant lactoperoxidase; a recombinant P-lactoglobulin, a recombinant a-lactalbumin, and a recombinant K-casein; a recombinant P-lactoglobulin, a recombinant a- lactalbumin, and a recombinant P-casein; a recombinant P-lactoglobulin, a recombinant a- lactal bumin, and a recombinant a-Sl -casein; a recombinant p-lactoglobulin, a recombinant a- lactalbumin, and a recombinant a-S2-casein; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant lactotransferrin; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant bovine serum albumin; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant lactoperoxidase; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant K-casem; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant P-casein; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant a-Sl -casein; a recombinant P-lactoglobulin, a recombinant GMP, and a recombinant a-S2-casein; a recombinant P-lactoglobulin, a recombinant lactotransferrin, and a recombinant bovine serum albumin; a recombinant P-lactoglobulin, a recombinant lactotransferrin, and a recombinant lactoperoxidase; a recombinant P-lactoglobulin, a recombinant lactotransferrin, and a recombinant K-casein; a recombinant p-lactoglobulin, a recombinant lactotransferrin, and a recombinant P-casein; a recombinant P-lactoglobulin, a recombinant lactotransferrin, and a recombinant a-Sl -casein; a recombinant P-lactoglobulin, a recombinant lactotransferrin, and a recombinant a-S2-casein; a recombinant P-lactoglobulin, a bovine serum albumin, and a recombinant lactoperoxidase; a recombinant P-lactoglobulin, a recombinant bovine serum albumin, and a recombinant K-casein; a recombinant P-lactoglobulin, a recombinant bovine serum albumin, and a recombinant P-casein; a recombinant P-lactoglobulin, a recombinant bovine serum albumin, and a recombinant a-Sl-casein; a recombinant P-lactoglobulin, a recombinant bovine serum albumin, and a recombinant a-S2-casein; a recombinant P- lactoglobulin, a recombinant lactoperoxidase, and a recombinant K-casein; a recombinant P- lactoglobulin, a recombinant lactoperoxidase, and a recombinant P-casein; a recombinant P- lactoglobulin, a recombinant lactoperoxidase, and a recombinant a-Sl -casein; a recombinant P-lactoglobulin, a recombinant lactoperoxidase, and a recombinant a-S2-casein; a recombinant P-lactoglobulin, a recombinant K-casein, and a recombinant P-casein; a recombinant P- lactoglobulin, a recombinant K-casein, and a recombinant a-Sl -casein; a recombinant P- lactoglobulin, a recombinant K-casein, and a recombinant a-S2-casein; a recombinant P- lactoglobulin, a recombinant P-casein, and a recombinant a-Sl -casein; a recombinant P-
17
SUBSTITUTE SHEET ( RULE 26) lactoglobulin, a recombinant 0-casein, and a recombinant a-S2-casein; a recombinant 0- lactoglobulin, a recombinant a-Sl -casein, and a recombinant a-S2-casein; a recombinant a- lactalbumm; a recombinant a-lactalbumin and a recombinant GMP; a recombinant a- lactalbumm and a recombinant lactotransferrin; a recombinant a-lactalbumin and a recombinant bovine serum albumin; a recombinant a-lactalbumin and a recombinant lactoperoxidase; a recombinant a-lactalbumin and a recombinant K-casein; a recombinant a- lactal bumin and a recombinant 0-casein; a recombinant a-lactalbumin and a recombinant a-S 1- casein; a recombinant a-lactalbumin and a recombinant a-S2-casein; a recombinant a- lactalbumm, a recombinant GMP, and a recombinant lactotransferrin; a recombinant a- lactalbumin, a recombinant GMP, and a recombinant bovine serum albumin; a recombinant a- lactalbumm, a recombinant GMP, and a recombinant lactoperoxidase; a recombinant a- lactalbumm, a recombinant GMP, and a recombinant K-casein; a recombinant a-lactalbumin, a recombinant GMP, and a recombinant 0-casein; a recombinant a-lactalbumin, a recombinant GMP, and a recombinant a-S 1 -casein; a recombinant a-lactalbumin, a recombinant GMP, and a recombinant a-S2-casein; a recombinant a-lactalbumin, a recombinant lactotransferrin, and a recombinant bovine serum albumin; a recombinant a-lactalbumin, a recombinant lactotransferrin, and a recombinant lactoperoxidase; a recombinant a-lactalbumin, a recombinant lactotransferrin, and a recombinant K-casein; a recombinant a-lactalbumin, a recombinant lactotransferrin, and a recombinant 0-casein; a recombinant a-lactalbumin, a recombinant lactotransferrin, and a recombinant a-S 1 -casein; a recombinant a-lactalbumin, a recombinant lactotransferrin, and a recombinant a-S2-casein; a recombinant a-lactalbumin, a recombinant bovine serum albumin, and a recombinant lactoperoxidase; a recombinant a- lactalbumm, a recombinant bovine serum albumin, and a recombinant K-casein; a recombinant a-lactalbumin, a recombinant bovine serum albumin, and a recombinant 0-casein; a recombinant a-lactalbumm, a recombinant bovine serum albumin, and a recombinant a-Sl- casein; a recombinant a-lactalbumin, a recombinant bovine serum albumin, and a recombinant a-S2-casein; a recombinant a-lactalbumin, a recombinant lactoperoxidase, and a recombinant K-casein; a recombinant a-lactalbumin, a recombinant lactoperoxidase, and a recombinant 0- casein; a recombinant a-lactalbumin, a recombinant lactoperoxidase, and a recombinant a-Sl- casein; a recombinant a-lactalbumin, a recombinant lactoperoxidase, and a recombinant a-S2- casein; a recombinant a-lactalbumm, a recombinant K-casein, and a recombinant 0-casein; a recombinant a-lactalbumin, a recombinant K-casein, and a recombinant a-S 1 -casein; a recombinant a-lactalbumin, a recombinant K-casein, and a recombinant a-S2-casein; a recombinant a-lactalbumin, a recombinant 0-casein, and a recombinant a-S 1 -casein; a
18
SUBSTITUTE SHEET ( RULE 26) recombinant a-lactalbumin, a recombinant P-casein, and a recombinant a-S2-casein; a recombinant a-lactalbumin, a recombinant a-Sl -casein, and a recombinant a-S2-casein; a recombinant GMP; a recombinant GMP and a recombinant lactotransferrin; a recombinant GMP and a recombinant bovine serum albumin; a recombinant GMP and a recombinant lactoperoxidase; a recombinant GMP and a recombinant K-casein; a recombinant GMP and a recombinant P-casein; a recombinant GMP and a recombinant a-Sl -casein; a recombinant GMP and a recombinant a-S2-casein; a recombinant GMP, a recombinant lactotransferrin, and a recombinant bovine serum albumin; a recombinant GMP, a recombinant lactotransferrin, and a recombinant lactoperoxidase; a recombinant GMP, a recombinant lactotransferrin, and a recombinant K-casein; a recombinant GMP, a recombinant lactotransferrin, and a recombinant P-casein; a recombinant GMP, a recombinant lactotransferrin, and a recombinant a-Sl -casein; a recombinant GMP, a recombinant lactotransferrin, and a recombinant a-S2-casein; a recombinant GMP, a recombinant bovine serum albumin, and a recombinant lactoperoxidase; a recombinant GMP, a recombinant bovine serum albumin, and a recombinant K-casein; a recombinant GMP, a recombinant bovine serum albumin, and a recombinant P-casein; a recombinant GMP, a recombinant bovine serum albumin, and a recombinant a-Sl -casein; a recombinant GMP, a recombinant bovine serum albumin, and a recombinant a-S2-casein; a recombinant GMP, a recombinant lactoperoxidase, and a recombinant K-casein; a recombinant GMP, a recombinant lactoperoxidase, and a recombinant P-casein; a recombinant GMP, a recombinant lactoperoxidase, and a recombinant a-Sl -casein; a recombinant GMP, a recombinant lactoperoxidase, and a recombinant a-S2-casein; a recombinant GMP, a recombinant K-casein, and a recombinant P-casein; a recombinant GMP, a recombinant K- casein, and a recombinant a-Sl -casein; a recombinant GMP, a recombinant K-casein, and a recombinant a-S2-casein; a recombinant GMP, a recombinant P-casein, and a recombinant a- S1 -casein; a recombinant GMP, a recombinant P-casein, and a recombinant a-S2-casein; a recombinant GMP, a recombinant a-Sl -casein, and recombinant a-S2-casein; a recombinant lactotransferrin; a recombinant lactotransferrin and a recombinant bovine serum albumin; a recombinant lactotransferrin and a recombinant lactoperoxidase; a recombinant lactotransferrin and a recombinant K-casein; a recombinant lactotransferrin and a recombinant P-casein; a recombinant lactotransferrin and a recombinant a-Sl -casein; a recombinant lactotransferrin and a recombinant a-S2-casein; a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant lactoperoxidase; a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant K-casein; a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant P-casein; a recombinant
19
SUBSTITUTE SHEET ( RULE 26) lactotransferrin, a recombinant bovine serum albumin, and a recombinant a-Sl -casein; a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant a-S2- casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant K- casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant P- casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant a- S1 -casein; a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant a-S2-casein; a recombinant lactotransferrin, a recombinant K-casein, and a recombinant p- casein; a recombinant lactotransferrin, a recombinant K-casein, and a recombinant a-S 1 -casein; a recombinant lactotransferrin, a recombinant K-casein, and a recombinant a-S2-casein; a recombinant lactotransferrin, a recombinant P-casein, and a recombinant a-Sl-casein; a recombinant lactotransferrin, a recombinant P-casein, and a recombinant a-S2-casein; a recombinant lactotransferrin, a recombinant a-S 1 -casein, and a recombinant a-S2-casein; a recombinant bovine serum albumin; a recombinant bovine serum albumin and a recombinant lactoperoxidase; a recombinant bovine serum albumin and a recombinant K-casein; a recombinant bovine serum albumin and a recombinant P-casem; a recombinant bovine serum albumin and a recombinant a-S 1 -casein; a recombinant bovine serum albumin and a recombinant a-S2-casein; a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant K-casein; a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant P-casein; a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant a-S 1 -casein; a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant a-S2-casein; a recombinant bovine serum albumin, a recombinant K-casein, and a recombinant P-casein; a recombinant bovine serum albumin, a recombinant K-casein, and a recombinant a-S 1 -casein; a recombinant bovine serum albumin, a recombinant K-casein, and a recombinant a-S2-casein; a recombinant bovine serum albumin, a recombinant P-casein, and a recombinant a-S 1 -casein; a recombinant bovine serum albumin, a recombinant P-casein, and a recombinant a-S2-casein; a recombinant bovine serum albumin, a recombinant a-S 1 -casein, and a recombinant a-S2-casein; a recombinant lactoperoxidase; a recombinant lactoperoxidase and a recombinant K-casein; a recombinant lactoperoxidase and a recombinant P-casein; a recombinant lactoperoxidase and a recombinant a-Sl-casein; a recombinant lactoperoxidase and a recombinant a-S2-casein; a recombinant lactoperoxidase, a recombinant K-casein, and a recombinant P-casein; a recombinant lactoperoxidase, a recombinant K-casein, and a recombinant a-Sl-casein; a recombinant lactoperoxidase, a recombinant K-casein, and a recombinant a-S2-casein; a recombinant lactoperoxidase, a recombinant P-casein, and a recombinant a-Sl-casein; a
20
SUBSTITUTE SHEET ( RULE 26) recombinant lactoperoxidase, a recombinant 0-casein, and a recombinant a-S2-casein; a recombinant lactoperoxidase, a recombinant a-Sl-casein, and a recombinant a-S2-casein; a recombinant K-casein; a recombinant K-casem and a recombinant -casein; a recombinant K- casein and a recombinant a-Sl -casein; a recombinant K-casein and a recombinant a-S2-casein; a recombinant K-casein, a recombinant |3-casein, and a recombinant a-Sl -casein; a recombinant K-casein, a recombinant P-casein, and a recombinant a-S2-casein; recombinant K-casein, a recombinant a-Sl -casein, and recombinant a-S2-casein; a recombinant p-casein; a recombinant P-casein and a recombinant a-Sl -casein; a recombinant P-casein and a recombinant a-S2- casein; a recombinant P-casein, a recombinant a-Sl -casein, and recombinant a-S2-casein; a recombinant a-Sl -casein; or a recombinant a-Sl -casein and a recombinant a-S2-casein.
[0060] Recombinant milk proteins, and methods for producing recombinant milk proteins (e.g., in a recombinant microbial host cell [e.g., in a recombinant filamentous fungal host cell]) are disclosed, for example, in U.S. Patent 9,924,728, issued March 27, 2018; U.S. publication US20190216106, published July 18, 2019; and PCT publication WO2020219596, published October 29, 2020.
[0061] The milk protein component comprised in a cosmetic or personal care composition according to any of the above may comprise or consist of a multimer (e g., dimer, trimer, tetramer) or polymer of recombinant milk protein monomers (i.e., molecules composed of repeated recombinant milk protein monomers that are covalently linked, either directly with each other or via intermediary molecules). The polymer of recombinant milk protein monomers may have the capacity to form a flexible film upon application of the cosmetic or personal care composition to a body surface.
[0062] The milk protein component comprised in a cosmetic or personal care composition according to any of the above may consist of whey protein and casein at a mass ratio of between about 10 to 0 and about 0 to 10 (e.g., about 10 to 0, about 10 to 1, about 9 to 1, about 8 to 1, about 7 to 1, about 6 to 1, about 5 to 1, about 4 to 1, about 3 to 1, about 2 to 1, about 1 to 1, about 1 to 2, about 1 to 3, about 1 to 4, about 1 to 5, about 1 to 6, about 1 to 7, about 1 to 8, about 1 to 9, about 1 to 10, about 0 to 10).
[0063] The milk protein component comprised in a cosmetic or personal care composition according to any of the above may comprise or consist of recombinant [3-lactoglobuhn and recombinant a-lactalbumin at a mass ratio of between about 10 to 0 and about 0 to 10 (e.g., about 10 to 0, about 10 to 1, about 9 to 1, about 8 to 1, about 7 to 1, about 6 to 1, about 5 to 1, about 4 to 1, about 3 to 1, about 2 to 1, about 1 to 1, about 1 to 2, about 1 to 3, about 1 to 4, about 1 to 5, about 1 to 6, about 1 to 7, about 1 to 8, about 1 to 9, about 1 to 10, about 0 to 10).
21
SUBSTITUTE SHEET ( RULE 26) [0064] The milk protein component comprised in a cosmetic or personal care composition according to any of the above may be comprised in a cosmetically or physiologically acceptable medium.
[0065] The milk protein component comprised in a cosmetic or personal care composition according to any of the above may be comprised in a coating layer around a core of the cosmetic or personal care composition. Such core may comprise an active agent (e.g., any of the other ingredients disclosed herein).
[0066] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be undenatured (i.e., not heat-insolubilized as shown by electrophoretic measurements).
[0067] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be hydrolyzed (e.g., via action of an endopeptidase [e.g., trypsin, chymotrypsin]). Such hydrolyzed recombinant milk protein may consist essentially of peptides having a molecular weight of between 200 to 5,000.
[0068] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be covalently or non- covalently associated with a lipid to form an amphiphilic complex. Non-limiting examples of suitable lipids for covalent association include fatty acids or acyls having carbon atom numbers of between 4 and 30, selected from fatty acids, fatty alcohols, and fatty amines, and derivatives thereof, and having chains that are unsaturated, linear, branched or cyclic. Non-limiting examples include heptanoic acid, octanoic acid, decanoic acid, lauric acid, myristic acid, palmitic acid, stearic acid, ncinoleic acid, oleic acid, linoleic acid, linolenic acid, caprylic acid, capric acid; the corresponding fatty alcohols and fatty amines; the derivatives of said fatty acids, fatty alcohols, and fatty amines; and mixtures thereof. The recombinant milk protein may comprise such lipid at a mass ratio of between about 1,000 to 1 and about 1 to 10. The lipid may be covalently linked to the recombinant milk protein via a monofunctional agent (which may be the fatty containing component [fatty monoacid, monoalcohol, or monoamine]) or a multifunctional grafting agent (which may be the fatty containing component [fatty polyacid, polyalcohol, or poly amine]).
[0069] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be covalently or non- covalently associated with a coloring agent (e.g., any of the coloring agents disclosed herein).
22
SUBSTITUTE SHEET ( RULE 26) [0070] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be covalently or non- covalently associated with a UVA and/or UVB light filtering or UVA and/or UVB light scattering agent. Non-limiting examples of UVA and/or UVB light filtering or UVA and/or UVB light scattering agents include BP-3 (2-hydroxy-4-methoxybenzophenone); cinnamoyl radical (optionally substituted by one or more alkoxy groups); p-dialkylaminobenzoyl radical; salicyloyl radical; sulphonyl radical originating from an isophthalylidene-camphor radical; acyl radical onginating from a carboxylic or sulphonic acid derived from benzylidenecamphor, 2-aiylbenzimidazoles, 2-arylbenzofurans, 2-arylbenzoxazoles, 2-arylbenzotriazoles, 2-aryhndoles, or hydroxybenzophenones; sulphonyl radical originating from a terephthalylidene-camphor radical; acyl radical derived from an absorber of carboxylic coumarin, monophenylcyanoacrylic, diphenylcyanoacrylic, or dibenzoylmethane structure (optionally substituted), and derivatives thereof.
[0071] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above may be comprised in a coacervate with a polysaccharide. The polysaccharide may be an anionic polysaccharide (i.e., a polysaccharide that has a negative total charge or linear charge density at a pH near the IEP of the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition), or a cationic polysaccharide (i.e., a polysacchande that has a positive total charge or linear charge density at the pH near the IEP of the recombinant milk protein comprised in the milk protein component of the cosmetic or personal care composition). Non-limiting examples of suitable anionic polysaccharides include polysaccharides having pendant carboxylic acid or sulfate groups, pectins (e.g., pectin from aloe, pectins from tobacco, pectins from apple pomace, pectins from citrus peel, pectins from plums, pectins from gooseberries sources, pectins from sugar beet), water soluble cellulose (e.g., sodium carboxymethyl cellulose, sulfated cellulose), seaweed hydrocolloid extracts (e.g., sodium alginate, propyleneglycol alginate, ammonium alginate, alginic acid, carageenans (e.g., iota carageenan, kappa carageenan, lambda carageenan)), microbial and agaroid gums and gum components (e.g., gellan gum, xanthan gum, gum Arabic, gum tragacanth, xanthan gum, ghatti gum, karaya gum, tragacanth gum, pullulan gum, scleroglucan, welan gum, rhamsan gum, okra gum, cellulose gum, carboxymethyl guar gum, carboxymethyl(hydroxypropyl) guar gum), starches containing carboxy groups (e.g., carboxymethyl starch), pullulanosic material containing carboxy groups, dextran materials containing carboxy groups, and derivatives thereof. Non-limiting examples of suitable cationic polysaccharides include chitosan,
23
SUBSTITUTE SHEET ( RULE 26) di ethylaminoethyl dextran, guar gum derivatives (e.g., guar hydroxypropyltrimonium chloride), quaternary nitrogen-containing cellulose ethers, copolymers of etherified cellulose, guar and starch, cationic cellulose, modified cationic cellulose polymers (e.g., polyquatemium 4, polyquatemium 10, polyquatemium 24, polyquatemium 28), laurdimonium hydroxypropyl oxyethyl cellulose, steardimonium hydroxyethyl cellulose, polysaccharides that comprise an amino group or an imine group, and derivatives thereof.
[0072] The recombinant milk protein may be comprised in the milk protein component comprised in a cosmetic or personal care product according to any of the above as a gel (e.g., a hydrogel).
Other Ingredients
[0073] The other ingredient that may be comprised in a cosmetic or personal care composition according to any of the above may be any known ingredient normally used in cosmetic or personal care compositions. The other ingredient should be of pharmaceutical or cosmetic grade, non-irritating on body surfaces, and non-toxic as used.
[0074] Non-limiting examples of other ingredients include: an aqueous solvent; a lipid; a wax; an emulsifying agent; a pH and/or ionic strength adjusting agent (i.e., a compound that can raise or lower the pH and/or the ionic strength of a solution; e.g., to balance the pH of the cosmetic or personal care composition to a pH of between 5 and 7); a buffering agent; a binding agent; a thickening agent; a filler agent (i.e., a colorless or white particle of any shape that is insoluble in the medium of the composition); a plasticizing agent; an anti-foam agent (e.g., to decrease foaming during compounding and handling of the composition); a foam stabilizer; a surfactant (e.g., a cationic, anionic, non-ionic, amphoteric, or zwitterionic surfactant); a gelling agent; a coloring agent (i.e., a molecule that comprises a chromophoric or chromogenic group; e.g., to color the composition and/or to color a body surface); an agent that imparts pearlescence; an oil-dispersing agent (i.e., a compound that can break up sebum on a body surface and spread it over the surface to minimize oily appearance and feel); a conditioning agent; an anti-oxidant (i.e., a molecule capable of slowing, reducing, inhibiting, or preventing the oxidation of other molecules); an anti-dandruff agent (i.e., a compound that is effective in reducing or eliminating dandruff); an anti-seborrheic dermatitis agent; an anti-psoriasis agent; an anti-acne agent (i.e., compound that is effective in the treatment of acne and/or symptoms associated therewith); a skin whitening agent (i.e., a compound that is effective in bleaching/ depigmenting skin color); a skin self-tanning agent (i.e., a compound that is effective in darkening skin color); a soothing agent (i.e., compound that reduces discomfort of skin
24
SUBSTITUTE SHEET ( RULE 26) and/or scalp, for example by soothing the feelings of itching); a cooling agent (i.e. , a compound that provides a sensation of cooling); a fragrance; an anti-aging agent (i.e., a compound that inhibits or reduces signs of aging, such as wrinkles, fine lines, and manifestations of photodamage [e.g., sun spots]); an UVA and/or UVB light absorbing agent; an UVA and/or UVB light scattering agent; an antibacterial agent (i.e., a compound that has either a killing or growth inhibiting effect upon bacteria); an anti-fungal agent (i.e., a compound that has either a killing or growth inhibiting effect upon fungi); an anti-inflammatoiy agent (i.e., a compound effective in treating inflammation); a salt; a humectant; a hair growth agent; a hair anti-static agent; an anti-viral agent (i.e., a compound capable of inhibiting cell entry or replication of a virus); a moisturizing agent (e.g., phytantriol, panthenol, vitamin F); an electrolyte; an herbal active agent (e.g., bioactive herbs, herbal extracts, tinctures, essential oils); and mixtures thereof.
[0075] Non-limiting examples of aqueous solvents include water, floral water, mineral water, Cl to C6 alcohols (e.g., ethyl alcohol, propanol, isopropyl alcohol, butanol, pentanol, hexanol, glycerol), glycols (e.g., ethylene glycol, butylene glycol, isoprene glycol, propylene glycol, polyethylene glycol), glycol ethers (e.g., methyl ether, ethyl ether, butyl ether, 2- butoxyethanol, ethylene glycol monoethyl ether, propylene glycol, diethylene glycol monoethyl ether, di ethylene glycol monomethyl ether), and mixtures thereof.
[0076] Non-limiting examples of lipids include fatty alcohols (e.g., linoleyl alcohol, linolenyl alcohol, oleyl alcohol), polyalcohols (e.g., glycol, polyethylene glycol, polypropylene glycol, glycerol), fatty acid esters (e.g., alkyl myristates [e.g., isopropyl myristate, butyl myristate, cetyl myristate], alkyl stearates [e.g., butyl stearate, hexadecyl stearate], alkyl palmitates [e.g., ethyl palmitate, isopropyl palmitate], alkyl lanolates [e.g., isopropyl lanolate, isocetyl lanolate], alkyl oleates [e.g., decyl oleate], alkyl octanoates, alkyl decanoates, alkyl ricinoleates [e.g., cetyl ricinoleate], laurates [e.g., hexyl laurate], adipates [e.g., isopropyl adipate]), fatty acids, fatty acid tnglycerides (e.g., triglycerides of C6-C30 fatty acids), natural oils (e.g., plant oils [e.g., sweet-almond oil, avocado oil, calophyllum oil, castor oil, sesame oil, jojoba oil, Karite oil, wheatgerm oil, sunflower oil, coconut oil, olive oil, com oil, soybean oil, marrow oil, grapeseed oil, hazelnut oil, apricot oil, avocado oil]), mineral and synthetic oils (e.g., paraffin oil, purcellin oil, perhydrosqualene, vaseline oil, silicone oils [e.g., dimethylpolysiloxanes, methylphenyl polysiloxanes], aliphatic hydrocarbons having more than 8 carbon atoms), fish oils (e.g., hoplostethus oil), and mixtures and derivatives thereof.
[0077] Non-limiting examples of waxes include ozokerite wax, montan wax; ouricury wax,
Japan wax, cork fibre wax, sugar cane wax, lanolins (e.g., lanolin, lanolin alcohol,
25
SUBSTITUTE SHEET ( RULE 26) hydrogenated lanolin, acetylated lanolin, lanolin wax, lanolin fatty acids), beeswax, candellila wax, microcrystalline wax, carnauba wax, cetyl alcohol, stearyl alcohol, cocoa butter; paraffins, ozokerites, petrolatum, vaselines, petroleum jelly, hydrogenated oils that are solid at 25C, mono-/di-/tri-glycerides that are solid at 25C, fatty esters that are solid at 25C, silicone waxes (e.g., methyloctadecane-oxypolysiloxane, poly(dimethyl-siloxy) stearoxysiloxane), fluoro waxes, stearic acid monoethanolamide, colophony and its derivatives (e g., glycol abietate, glycerol abietate), hydrogenated oils that are solid at 25C, jojoba oil, sucroglycerides, calcium oleates, magnesium oleates, zirconium oleates, aluminum oleates, myristates, lanolates, stearates, dihydroxystearates, and mixtures and derivatives thereof.
[0078] Non-limiting examples of emulsifying agents include polyglycerin esters, sorbitan esters, partially esterified glycerides, and derivatives and mixtures thereof.
[0079] Non-limiting examples of pH adjusting agents include organic acids, mono- or triethanolamine, ammonia, sodium phosphate, sodium carbonate, phosphoric acid, hydrochloric acid, lactic acid, tartaric acid, acetic acid, citric acid, and mixtures thereof.
[0080] Non-limiting examples of buffering agents include ethylenediaminetetracetic acid (EDTA), sodium salt of EDTA, sodium chloride, sodium phosphate, diazolidinyl urea, iodopropynyl butylcarbamate, and mixtures thereof.
[0081] Non-limiting examples of binding agents include silicone resins (e.g., silanes, siloxanes [e.g., polydimethylsiloxane (PDMS)], siloxysilicates [e.g., tnmethylsiloxysilicate], silsesqui oxanes [e.g., polymethylsilsesqui oxane]), dimethicone silylate/isododecane, polyglyceryyl-2 triisostearate, dry binders (e.g., zinc, lithium, magnesium stearate), and derivatives and mixtures thereof.
[0082] Non-limiting examples of thickeners include salts, PEG-55, propylene glycol oleate, PEG- 120 methyl lucose ioleate, cellulose derivatives [e.g., carboxymethyl cellulose, hydroxypropylmethylcellulose, hydroxyethylcellulose], and derivatives and mixtures thereof. [0083] Non-limiting examples of fdlers include silica microspheres (e.g., hollow silica microspheres, acrylic polymer microspheres [e.g., microspheres made of crosslinked acrylate copolymer, microspheres made of polymethyl methacrylate]); polyurethane powders (e.g., powder of a copolymer of hexamethylene diisocyante and of trimethylol hexyl lactone); elastomeric crosslinked organopolysiloxane spherical powders; carnauba or paraffin wax microbeads; metal soaps in powder form (e.g., metal soaps of fatty acids containing from 12 to 22 carbons, where the metal may be zinc or magnesium, and the fatty acid may be lauric acid, myristic acid, stearic acid, and palamitic acid [e.g., zinc laurate, magnesium stearate, magnesium myristate, zinc stearate]), talcs or hydrated magnesium silicates; micas or
26
SUBSTITUTE SHEET ( RULE 26) aluminosilicates (e.g., micas of natural origin [e.g., muscovite, margarite, roscoelite, lepidolite, biotite] or of synthetic origin); clays (e.g., sericites); kaolin or hydrated aluminum silicate; boron nitrides; powders of tetrafluoroethylene polymers; precipitated calcium carbonate, magnesium carbonate, or magnesium hydrogen carbonate; hydroxyapatite; powders of nonexpanded synthetic polymers (e.g., polyethylene, polyesters [e.g., polyethylene isophthalate or terephthalate], polyamides [e.g., Nylon]); powders of spheronized, crosslinked or noncrosslinked synthetic polymers (e.g., polyamide powders [e.g., poly-P-alanine powder, Nylon powder, polyacrylic acid powder, polymethacrylic acid powder, powders of polystyrene crosslinked with divinylbenzene, silicone resin powders); bismuth oxychloride powders; powders of organic materials of natural origin (e.g., starches [e.g., com starch, wheat starch, rice starch]); and mixtures thereof.
[0084] Non-limiting examples of plasticizing agents include glycerin, sorbitol, a non-toxic glycol or polyglycol (e.g., propylene dipropylene or other C2-C4 glycol), and derivatives and mixtures thereof.
[0085] Non-limiting examples of surfactants include alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkyl aryl sulfonates, alkyl succinates, alkyl sulfosuccinates, N-alkoyl sarcosinates, acyl taurates, acyl isothionates, alkyl phosphates, alkyl ether phosphates, alkyl ether carboxylates, alpha-olefin sulfonates (e.g., alkaline and earth alkaline metal salts [e.g., sodium, potassium, magnesium, calcium, ammonium, and triethanolamine salts]), alkyl betaines, alkyl amido propyle betaines, alkyl sulfobetaines, alkyl glycinates, alkyl carboxy glycinates, alkyl amphoacetates, alky propionates, alkyl amphodiacetates, alkyl dipropionates, reaction products of aliphatic alcohols or alkyl phenols having 6 to 20 C atoms in the alkyl chain with ethylene oxide and/or propylene oxide, quaternary ammonium compounds (e.g., cetyl trimethyl ammonium chloride), and derivatives and mixtures thereof.
[0086] Non-limiting examples of coloring agents include dyes, lacquers, mineral pigments, organic pigments, nacres (i.e., colored particles of any form, which may or may not be iridescent, especially produced by certain molluses in their shell, or alternatively synthesized, and which have a color effect via optical interference), reflective particles (i.e., particles that impart sparkle), FD&C Blue No. 1 (Brilliant Blue), FD&C Blue No. 2 (Indigotine), FD&C Green No. 3 (Fast Green), FD&C Red No. 3 (Erythrosine), FD&C Red No. 40 (Allura Red), FD&C Yellow No. 5 (Tartrazine), FD&C Yellow No. 6 (Sunset Yellow), annatto extract, anthocyanis, aronia/redfruit, beet juice, beet powder, beta-carotene, beta-apo-8-carotenal, black currant, burnt sugar, canthaxanthin, caramel, carbo medicinalis, carmine, carmine/beta- carotene, carmine blue, carminic acid, carrot, carrot oil, chlorophyll, chlorophyllin, cochineal
27
SUBSTITUTE SHEET ( RULE 26) extract, copper-chlorophyll, copper-chlorophyllin, curcumin, curcumin/Cu-chlorophyllin, elderberry, grape, grape skin extract, hibiscus, lutein, mixed carotenoids, paprika, paprika extract, paprika oleoresin, riboflavin, saffron, spinach, stinging nettle, titanium dioxide, turmeric, titanium dioxide, zirconium oxide, cerium oxide, zinc oxide, iron (black, yellow or red) oxide, chromium oxide, sericites, micas, talcs, kaolin, manganese violet, ultramarine blue, chromium hydrate and ferric blue, metal powders (e.g., aluminum powder, copper powder), cochineal carmine, carotenoids, curcumin, porphyrin pigment, vegetable carbon black, organic pigments of azo dyes, anthraquinone dyes, indigoid dyes, xanthene dyes, pyrene dyes, quinoline dyes, triphenylmethane dyes, fluorane dyes, D&C certified pigments (e.g., D&C Blue No. 4, D&C Brown No. 1, D&C Green No. 5, D&C Green No. 6, D&C Orange No. 4, D&C Orange No. 5, D&C Orange No. 10, D&C Orange No. 11, D&C Red No. 6, D&C Red No. 7, D&C Red No. 17, D&C Red No. 21, D&C Red No. 22, D&C Red No. 27, D&C Red No. 28, D&C Red No. 30, D&C Red No. 31, D&C Red No. 33, D&C Red No. 34, D&C Red No. 36, D&C Violet No. 2, D&C Yellow No. 7, D&C Yellow No. 8, D&C Yellow No. 10, D&C Yellow No. 11, FD&C Blue No. 1, FD&C Green No. 3, FD&C Red No. 40, FD&C Yellow No. 5, FD&C Yellow No. 6), titanium mica coated with an iron oxide, mica coated with bismuth oxychloride, titanium mica coated with chromium oxide, pigments based on bismuth oxychloride, and particles comprising a borosilicate substrate coated with titanium oxide, and derivatives and mixtures thereof.
[0087] Non-limiting examples of agents that impart pearlescence include bismuth oxychloride, titanium mica, and guanine crystals.
[0088] Non-limiting examples of oil-dispersing agents include rhamnolipid biosurfactants, sophorolipid biosurfactants, polylipid protein sulfates, and derivatives and mixtures thereof.
[0089] Non-limiting examples of conditioning agents include silicone compounds (e.g., polyalkyl siloxanes, polyaryl siloxanes, polyarylalkyl siloxanes, polyether siloxanes, silicone resins).
[0090] Non-limiting examples of anti-oxidants include alpha hydroxy acids (e.g., glycolic acid, citric acid, lactic acid, malic acid, mandelic acid, ascorbic acid, alpha-hydroxybutyric acid, alpha-hydroxyisobutyric acid, alpha-hydroxyisocaproic acid, atrrolactic acid, alphahydroxyisovaleric acid, ethyl pyruvate, galacturonic acid, glucopehtonic acid, glucopheptono 1,4-lactone, gluconic acid, gluconolactone, glucuronic acid, glucurronolactone, glycolic acid, isopropyl pyruvate, methyl pyruvate, mucic acid, pyruvia acid, saccharic acid, saccanc acid 1,4-lactone, tartaric acid, tartronic acid), chelating agents (e.g., metal chelators) such as ethylenediaminetetraacetic acid (EDTA), citrate, ethylene glycol tetraacetic acid (EGTA), 1,2-
28
SUBSTITUTE SHEET ( RULE 26) bis(o-aminophenoxy)ethane-N,N,N' ,N' -tetraacetic acid (BAPTA), diethylene triamine pentaacetic acid (DTP A), 2, 3-dimercapto-l -propanesulfonic acid (DMPS), dimercaptosuccinic acid (DMSA), a-lipoic acid, salicylaldehyde isonicotinoyl hydrazone (SIH), hexyl thioethylamine hydrochloride (HTA), desferrioxamine, ascorbic acid (vitamin C), cysteine, glutathione, dihydrolipoic acid, 2-mercaptoethane sulfonic acid, 2-mercaptobenzimidazole sulfonic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, sodium metabisulfite, vitamin E isomers (e.g., a-, |3-, y-, and 8-tocopherols; a-, 0-, y-, and 5-tocotrienols), polyphenols (e.g., 2-tert-butyl-4-methyl phenol, 2-tert-butyl-5 -methyl phenol, 2-tert-butyl-6-methyl phenol), butylated hydroxyanisole (BHA) (e.g., 2-tert-butyl-4-hydroxyanisole, 3-tert-butyl-4- hydroxyanisole), butylhydroxytoluene (BHT), tert-butylhydroquinone (TBHQ), ascorbyl palmitate, n-propyl gallate, and derivatives, salts, and mixtures thereof.
[0091] Non-limiting examples of suitable anti-dandruff agents, anti-seborrheic dermatitis agents, and anti-psoriasis agents include zinc pyrithione, selenium sulfide, sulfur, sulfonated shale oil, salicylic acid, coal tar, povidone-iodine, imidazoles (e.g., ketoconazole, dichlorophenyl imidazolodioxalan, clotrimazole, itraconazoie, miconazole, climbazole, tioconazole, sulconazole, butoconazole, fluconazole, miconazolenitrite, anthralin), piroctone olamine (Octopirox), selenium sulfide, ciclopirox olamine, anti-psoriasis agents, vitamin A analogs, corticosteroids, and derivatives and mixtures thereof.
[0092] Non-limiting examples of anti-acne agents include to keratolytics (e.g., salicylic acid, derivatives of salicylic acid, resorcinol); retinoids (e.g., retinoic acid, tretinoin, adapalene, tazarotene); sulfur-containing D- and L-amino acids and their derivatives and salts; lipoic acid; antibiotics and antimicrobials (e.g., lantibiotics [e.g., nisin, subtilin, epidermin, gallidermin], defensins, attacins [e.g., sarcotoxin], cecropins [e.g, cecropin A, bactericidin, lepidopteran], magainins, melittins, histatins, brevinins, novispirins, benzoyl peroxide, triclosan, chlorhexidine gluconate, octopirox, tetracycline, 2,4,4' -trichloro-2' -hydroxy diphenyl ether, 3,4,4' -tnchlorobanilide, nicotinamide, tea tree oil, rofecoxib, azelaic acid, phenoxyethanol, phenoxypropanol, phenoxisopropanol, ethyl acetate, clindamycin, erythromycin, meclocycline); sebostats (e.g., flavonoids); bile salts (e.g., scymnol sulfate and its derivatives, deoxy cholate, cholate); and derivatives and combinations thereof.
[0093] Non-limiting examples of skin whitening agents include hydrogen peroxide, zinc peroxide, sodium peroxide, hydroquinone, 4-isopropylcatechol, hydroquinone monobenzyl ether, kojic acid, lactic acid, ascorbyl acid and derivatives (e.g., magnesium ascorbyl
29
SUBSTITUTE SHEET ( RULE 26) phosphate), arbutin, licorice root, sugar amines (e.g., glucosamine, N-acetyl glucosamine, glucosamine sulfate, mannosamine, N-acetyl mannosamine, galactosamine, N-acetyl galactosamine), N-acyl amino acids (e.g., N-acyl phenylalanine, N-acyl tyrosine, N- undecylenoyl-L-phenylalanine), soy isoflavones, retinoids (e.g., retinol), kojic dipalmitate, arbutin, transexamic acid, vitamins (e.g., niacin, vitamin C), azelaic acid, linolenic acid, linoleic acid, placertia, natural extracts (e.g., chamomile, green tea, soy extract), and derivatives and combinations thereof.
[0094] Non-limiting examples of skin self-tanning agents include dihydroxy acetone (DHA), glyceryl aldehyde, tyrosine and tyrosine derivatives (e.g., malyltyrosine, tyrosine glucosinate, ethyl tyrosine), phospho-DOPA, indoles, and derivatives and combinations thereof.
[0095] Non-limiting examples of soothing agents include aloe, avocado oil, green tea extract, hops extract, chamomile extract, colloidal oatmeal, calamine, cucumber extract, sodium palmate, sodium palm kemelate, butyrospermum parkii (i.e., shea butter), menthe piperita (i.e., peppermint) leaf oil, sericin, pyridoxine (a form of vitamin B6), retinyl palmitate and/or other forms of vitamin A, tocopheryl acetate and/or other forms of vitamin E, lauryl laurate, hyaluronic acid, aloe barbadensis leaf juice powder, euterpe oleracea (i.e., acai berry) fruit extract, riboflavin (i.e., vitamin B2), thiamin HC1 and/or other forms of vitamin Bl, and variations and mixtures thereof.
[0096] Non-limiting examples of cooling agents include WS-3, WS-23, menthol, 3- substituted-P-menthanes, N-substituted-P-menthane-3-carboxamides, isopulegol, 1,2- alkanediols (e.g., 3-(l-menthoxy)propane-l,2-diol, 3-(l-menthoxy)-2-methylpropane-l,2- diol, p-menthane-2,3-diol, p-menthane-3,8-diol), 6-isopropyl-9-methyl-l,4- dioxaspiro[4,5]decane-2-methanol, menthyl succinate and its alkaline earth metal salts, trimethylcyclohexanol, N-ethyl-2-isopropyl-5-methylcyclohexanecarboxamide, Japanese mint oil, peppermint oil, menthone, menthone glycerol ketal, menthyl lactate, 3-(l-menthoxy)ethan- l-ol, 3-(l-menthoxy)propan-l-ol, 3-(l-menthoxy)butan-l-ol, 1 -menthylacetic acid N-ethyl amide, l-menthyl-4-hydroxypentanoate, l-menthyl-3-hydroxybutyrate, N,2,3-trimethyl-2-(l- methylethylj-butanamide, n-ethyl-t-2-c-6 nonadienamide, N,N-dimethyl menthyl succinamide, menthyl pyrrolidone carboxylate, and derivatives and mixtures thereof.
[0097] Non-limiting exemples of fragrances include bisabolol; 2,4-dimethyl-3- cyclohexene-l-carbaldehyde; isocyclocitral; menthone; isomenthone; ROMASCONE® (methyl 2, 2-dimethyl-6-methylene-l -cyclohexanecarboxylate); nerone; terpineol; dihydroterpineol; terpenyl acetate; dihydroterpenyl acetate; dipentene; eucalyptol; hexylate;
30
SUBSTITUTE SHEET ( RULE 26) rose oxide; PERYCOROLLE® ((S)-l,8-p-menthadiene-7-ol); l-p-menthene-4-ol; (lRS,3RS,4SR)-3-p-mentanyl acetate; (lR,2S,4R)-4,6,6-trimethyl-bicyclo[3,l,l]heptan-2-ol; DOREMOX® (tetrahydro-4-methyl-2-phenyl-2H-pyran); cyclohexyl acetate; cyclanol acetate; Fructalate (1,4-cyclohexane di ethyldicarboxylate); KOUMALACTONE® ((3ARS,6SR,7ASR)-perhydro-3,6-dimethyl-benzo[B]furan-2-one); Natactone ((6R)- perhydro-3,6-dimethyl-benzo[B]furan-2-one); 2,4,6-trimethyl-4-phenyl-l,3-dioxane; 2,4,6- trimethyl-3-cyclohexene-l-carbaldehyde; (E)-3-methyl-5-(2,2,3-trimethyl-3-cyclopenten-l- yl)-4-penten-2-ol; (T R,E)-2-ethyl-4-(2' ,2' ,3' -trimethyl-3' -cyclopenten-T -yl)-2- buten-l-ol; POLYSANTOL® ((T R,E)-3,3-dimethyl-5-(2' ,2' ,3' -trimethyl-3' - cyclopenten-1' -yl)-4-penten-2-ol); fleuramone; PARADISONE® (methyl-(lR)-cis-3-oxo-2- pentyl-l-cyclopentane acetate); Veloutone (2,2,5-Trimethyl-5-pentyl-l-cyclopentanone); NIRVANOL® (3,3-dimethyl-5-(2,2,3-trimethyl-3-cyclopenten-l-yl)-4-penten-2-ol); 3- methyl-5-(2,2,3-trimethyl-3-cyclopenten-l-yl)-2-pentanol; damascenes; NEOBUTENONE® (l-(5,5-dimethyl-l-cyclohexen-l-yl)-4-penten-l-one); nectalactone ((!' R)-2-[2-(4' - methyl-3' -cyclohexen-1' -yl)propyl]cyclopentanone); alpha-ionone; beta-ionone; damascenone; DYNASCONE® (mixture of l-(5,5-dimethyl-l-cyclohexen-l-yl)-4-penten-l- one and l-(3,3-dimethyl-l-cyclohexen-l-yl)-4-penten-l-one); DORINONE® beta (l-(2,6,6- trimethyl-l-cyclohexen-l-yl)-2-buten-l-one); ROMANDOLIDE® ((1S,1' R)-[l-(3' ,3' - Dimethyl-1' -cyclohexyl)ethoxycarbonyl]methyl propanoate); 2-tert-butyl-l -cyclohexyl acetate; LIMB ANOL® (l-(2,2,3,6-tetramethyl-cyclohexyl)-3-hexanol); trans- 1 -(2,2,6- trimethyl-l-cyclohexyl)-3-hexanol; (E)-3-methyl-4-(2,6,6-trimethyl-2-cyclohexen-l-yl)-3- buten-2-one; terpenyl isobutyrate; LORYSIA® (4-(l,l-dimethylethyl)-l-cyclohexyl acetate); 8-methoxy-l-p-menthene; HELVETOLIDE® ((1S,T R)-2-[l-(3' ,3' -dimethyl-1' - cyclohexyl)ethoxy]-2-methylpropyl propanoate); para tert-butylcyclohexanone; menthenethiol; l-methyl-4-(4-methyl-3-pentenyl)-3-cyclohexene-l-carbaldehyde; allyl cyclohexylpropionate; cyclohexyl salicylate; Methyl cedryl ketone; Verdylate; vetyverol; vetyverone; l-(octahydro-2,3,8,8-tetramethyl-2-naphtalenyl)-l-ethanone; (5RS,9RS,10SR)-
2.6.9.10-tetramethyl-l-oxaspiro[4.5]deca-3,6-diene and the (5RS,9SR,10RS) isomer; 6-ethyl-
2.10.10-tnmethyl-I-oxaspiro[4.5]deca-3,6-diene; l,2,3,5,6,7-hexahydro-l,l,2,3,3- pentamethyl-4-indenone; HIVERNAL® (a mixture of 3-(3,3-dimethyl-5-indanyl)propanal and
31
SUBSTITUTE SHEET ( RULE 26) 3-(l,l-dimethyl-5-indanyl)propanal); Rhubofix® (3' ,4-dimethyl- tricyclo[6.2.1.0(2,7)]undec-4-ene-9-spiro-2z -oxirane); 9/10-ethyldiene-3- oxatricyclo[6.2.1 ,0(2,7)]undecane; POLYWOOD® (perhydro-5,5,8A-tnmethyl-2- naphthalenyl acetate); octalynol; CETALOX® (dodecahydro-3a,6,6,9a-tetramethyl- naphtho[2, l-b]furan); tricyclo[5.2.1 ,0(2,6)]dec-3-en-8-yl acetate and tricyclo[5.2.1 ,0(2,6)]dec-
4-en-8-yl acetate as well as tricyclo[5.2.1.0(2,6)]dec-3-en-8-yl propanoate and tricyclo[5.2.1.0(2,6)]dec-4-en-8-yl propanoate; camphor; borneol; isobomyl acetate; 8- isopropyl-6-methyl-bicyclo[2.2.2]oct-5-ene-2-carbaldehyde; camphopinene; cedramber (8- methoxy-2,6,6,8-tetramethyl-tricyclo[5.3.1.0(l,5)]undecane); cedrene; cedrenol; cedrol; FLOREX® (mixture of 9-ethylidene-3-oxatricyclo[6.2. 1.0(2, 7)]undecan-4-one and 10- ethylidene-3-oxatricyclo[6.2.1.0(2,7)]undecan-4-one); 3-methoxy-7,7-dimethyl-10- methylene-bicyclo[4.3.1]decane; CEDROXYDE® (trimethyl-13-oxabicyclo-[10.1.0]-trideca- 4,8-diene); Ambrettolide LG ((E)-9-hexadecen- 16-olide); HABANOLIDE® (pentadecenolide); muscenone (3-methyl-(4/5)-cyclopentadecenone); muscone; EXALTOLIDE® (pentadecanolide); EXALTONE® (cyclopentadecanone); (1- ethoxyethoxy)cyclododecane; Astrotone; LILIAL®; rosinol; and derivatives and combinations thereof.
[0098] Non-limiting examples of anti-aging agents include vitamins (e.g., vitamin E, vitamin A, vitamin C (ascorbic acid), vitamin B, vitamin E acetate, vitamin C palmitate), hydroxy acids (e.g., alpha-hydroxy acids [e.g., glycolic acid, lactic acid, 2-hydroxy butanoic acid, malic acid, citric acid tartaric acid, alpha-hydroxyethanoic acid, hydroxycaprylic acid], beta hydroxy acids [e.g., beta-hydroxybutyric acid, beta-phenyl-lactic acid, beta-phenylpyruvic acid, salicylic acid], polyhydroxy acids [e.g., gluconolactone (G4)]), gamma-linolenic acid, flavonoids (e.g., quercetin, hesperidin, quercitrin, rutin, tangeritin, epicatechin), CoQlO, retinoids, other proteins (e.g., collagen, soybean protein) and protein hydrolysates, bioactive agents (e.g., epidermal growth factor), botanical extracts (e.g., green tea, soy, milk thistle, algae, aloe, angelica, bitter orange, coffee, goldthread, grapefruit, hoellen, honeysuckle, Job's tears, lithospermum, mulberry, peony, puerarua, rice, safflower), and derivatives and combinations thereof.
[0099] Non-limiting examples of UVA and/or UVB light absorbing agents include UV absorbers of benzoic acid system (e.g., para-aminobenzoic acid (PABA); PABA monoglycerin ester; N,N-dipropoxy PABA ethyl ester; N,N-diethoxy PABA ethyl ester; N,N-dimethyl PABA ethyl ester; N,N-dimethyl PABA butyl ester; N,N-dimethyl PABA methyl ester); UV
32
SUBSTITUTE SHEET ( RULE 26) absorbers of anthranilic acid system (e.g., homomenthyl-N-acetyl anthranilate); UV absorbers of salicylic acid system (e.g., amyl salicylate; menthyl salicylate; homomenthyl salicylate; octyl salicylate; phenyl salicylate; benzyl salicylate; p-isopropanol phenyl salicylate); UV absorbers of cinnamic acid system (e.g., octyl cinnamate; ethyl-4-isopropyl cinnamate; methyl- 2,5-diisopropyl cinnamate; ethyl-2,4-diisopropyl cinnamate; methyl-2,4-diisopropyl cinnamate; propyl-p-methoxy cinnamate; isopropyl-p-methoxy cinnamate; isoamyl-p- methoxy cinnamate; octyl-p-methoxy cinnamate(2-ethylhexyl-p-methoxy cinnamate); 2- ethoxyethyl-p-methoxy cinnamate; cyclohexyl-p-methoxy cinnamate; ethyl-a-cyano-0-phenyl cinnamate; 2-ethylhexyl-a-cyano-(3-phenyl cinnamate; glyceryl mono-2-ethylhexanoyl-dipara- methoxy cinnamate; methyl-bis(trimethylsiloxane)silylisopentyl trimethoxy cinnamate); 3-(4 -methylbenzylidene)-d,l-camphor; 3 -benzylidene-d.l -camphor: urocanic acid; urocanic acid ethyl ester; 2-phenyl-5-methylbenzoxazole; 2,2' -hydroxy-5-methylphenylbenzotriazole; 2-(2
-hydroxy-5' -t-octylphenyl)benzotriazole; 2-(2' -hydroxy-5' - methylphenylbenzotriazole; dibenzaladine; dianisoylmethane; 4-methoxy-4' -t- butyldibenzoylmethane; 5-(3,3-dimethyl-2-norbomylidene)-3-pentane-2-one; dimorpholinopyridazinone; and derivatives and combinations thereof.
[0100] Non-limiting examples of UVA and/or UVB light scattering agents include titanium oxide, particulate titanium oxide, zinc oxide, particulate zinc oxide, ferric oxide, particulate ferric oxide, ceric oxide, and derivatives and combinations thereof.
[0101] Non-limiting examples of antibacterial agents include macrolides or ketolides (e.g., erythromycin, azithromycin, clarithromycin, telithromycin); beta-lactams (e.g., penicillin, cephalosporin, carbapenems [e.g., carbapenem, imipenem, meropenem]); monolactams (e.g., penicillin G, penicillin V, methicillin, oxacillin, cioxacillin, dicloxacillin, nafcillin, ampicillin, amoxicillin, carbenicillin, ticarcillin, meziocillin, piperacillin, azlocillin, temocillin, cepalothin, cephapirin, cephradine, cephaloridine, cefazolin, cefamandole, cefuroxime, cephalexin, cefprozil, cefaclor, loracarbef, cefoxitin, cefmetazole, cefotaxime, ceftizoxime, ceftriaxone, cefoperazone, ceftazidime, cefixime, cefpodoxime, ceftibuten, cefdinir, cefpirome, cefepime, and astreonam; quinolones such as nalidixic acid, oxolinic acid, norfloxacin, pefloxacin, enoxacin, ofloxacin, levofloxacin, ciprofloxacin, temafloxacin, lomefloxacin, fleroxacin, grepafloxacin, sparfloxacin, trovafloxacin, clinafloxacin, gatifloxacin, moxifloxacin, sitafloxacin, besifloxacin, besifloxaxin, chntafloxacin, ganefloxacin, Gemifloxacin, pazufloxacin); antibacterial sulfonamides and antibacterial
33
SUBSTITUTE SHEET ( RULE 26) sulphanilamides (e.g., para-aminobenzoic acid, sulfadiazine, sulfisoxazole, sulfamethoxazole, sulfathalidine); aminoglycosides (e.g., streptomycin, neomycin, kanamycin, paromycin, gentamicin, tobramycin, amikacin, netilmicin, spectinomycin, sisomicin, dibekalin, isepamicin); tetracyclines (e.g., tetracycline, chlortetracycline, demeclocy cline, minocycline, oxy tetracycline, methacycline, doxycycline); rifamycins (e.g., rifampicin [i.e., rifampin], rifapentine, rifabutin, bezoxazinorifamycin, rifaximin); lincosamides (e.g., lincomycin, clindamycin); glycopeptides (e.g., vancomycin, teicoplanin); streptogramins (e.g., quinupristin, daflopristin); oxazolidinones (e.g., linezolid); polymyxin, colistin, colymycin; trimethoprim, bacitracin, and phosphonomycin.
[0102] Non-limiting examples of antifungal agents include azoles (e.g., Fluconazole, Isavuconazole, Itraconazole, Ketoconazole (or a pyrithione salt thereof [e.g., zinc pyrithione, sodium pyrithione, potassium pyrithione, lithium pyrithione, ammonium pyrithione, copper pyrithione, calcium pyrithione, magnesium pyrithione, strontium pyrithione, silver pyrithione, gold pyrithione, manganese pyrithione, ethanolamine salt, chitosan salt, disulfide salt of pyrithione]), Miconazole, Clortrimazole, Voriconazole, Posaconazole, Ravuconazole, Ciclopirox, etc.), polyenes (e.g., natamycin, lucensomycin, nystatin, amphotericin B, etc.), echinocandins (e.g., Canci das), pradimicins (e.g., beanomicins, nikkomycins, sordarins, allylamines, etc.), Triclosan, Piroctone, fenpropimorph, terbinafme, an antifungal agent effective against the fungus of genus Malassezia (e.g., Malassezia globose) or of genus Trichophyton (e.g., Trichophyton rubrum), and derivatives and analogs thereof.
[0103] Non-limiting examples of anti-inflammatory agents include steroidal antiinflammatory (e.g., 21-acetoxypregnenolone, alclometasone, algestone, amcinonide, beclomethasone, betamethasone, budesonide, chloroprednisone, clobetasol, clobetansone, clocortolone, cloprednol, corticosterone, cortisone, cortivazol, deflazacort, desonide, desoximetasone, dexamethasone, diflorasone, diflucortolone, difluprednate, enoxolone, fluazacort, flucloronide, flumethasone flunisolide, fluocinolone acetonide, fluocinonide, fluocortin butyl, fluocortolone, fluoromethoIone, fluperolone acetate, fluprednidene acetate, fluprednisolone, flurandrenolide, fluticasone propionate, formocortal, halcinonide, halobetasol propionate, halometasone, halopredone acetate, hydrocortamate, hydrocortisone, loteprednol etabonate, mazipredone, medrysone, meprednisone, methylprednisolone, mometasone furcate, paramethosone, prednicarbate, prednisolone, prednisolone 25 -di ethylamino-acetate, prednisolone sodium phosphate, prednisone, prednival, prednylidene, rimexolone, tixocortol, triamcinolone, triamcinolone acetonide, triamcinolone benetonide, triamcinolone hexacetonide), non-steroidal antiinflammatory drugs (NSAIDs) (e.g., COX inhibitors [COX-1
34
SUBSTITUTE SHEET ( RULE 26) or COX nonspecific inhibitors; e.g., salicylic acid derivatives (e.g., aspirin, sodium salicylate, choline magnesium trisalicylate, salicylate, diflunisal, sulfasalazine, olsalazine), paraaminophenol derivatives (e.g., acetaminophen); indole and indene acetic acids (e.g., indomethacin, sulindac); heteroaryl acetic acids (e.g, tolmetin, diclofenac, ketorolac); arylpropionic acids (e.g., ibuprofen, naproxen, flurbiprofen, ketoprofen, fenoprofen, oxaprozin); anthranilic acids (fenamates; e.g., mefenamic acid, meloxicam); enolic acids (e.g., oxicams [e.g., piroxicam, meloxicam]); alkanones (e.g., nabumetone)], selective COX-2 inhibitors [e.g., diarylsubstituted furanones (e.g., refecoxib); diaryl-substituted pyrazoles (e.g., celecoxib); indole acetic acids (e.g., etodolac, sulfonanilides [e.g., nimesulide])]), and derivatives and combinations thereof.
Attribute
[0104] A cosmetic or personal care composition according to any of the above may have an attribute (e.g., any one of the attributes disclosed herein, or combination of two or more of the attributes disclosed herein) that is between 50% and 150%, 140%, 130%, 120%, 110%, 100%, 90%, 80%, 70%, or 60%; between 60% and 150%, 140%, 130%, 120%, 110%, 100%, 90%, 80%, or 70%; between 70% and 150%, 140%, 130%, 120%, 110%, 100%, 90%, or 80%; between 80% and 150%, 140%, 130%, 120%, 110%, 100%, or 90%; between 90% and 150%, 140%, 130%, 120%, 110%, or 100%; between 100% and 150%, 140%, 130%, 120%, or 110%; between 110% and 150%, 140%, 130%, or 120%; between 120% and 150%, 140%, or 130%; between 130% and 150%, or 140%; or between 140% and 150% of such attribute of a corresponding cosmetic or personal care composition, or that is at least 1.5-fold, at least 2-fold, at least 3-fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, at least 10-fold, at least 15-fold, or at least 20-fold of such attribute of a corresponding cosmetic or personal care composition.
[0105] Non-limiting examples of attributes include film forming (e.g., for even spreading on a body surface); film elasticity (e.g., elasticity of film formed on body surface); film flexibility (e.g., flexibility of film formed on body surface); adhesion to body surface (e.g., binding to sebum secreted from skin or keratin in hair to influence duration of wear); foam forming (e.g., foaming of hair shampoo); gelling; moisturizing of body surface; emulsifying (e.g., to stabilize oil droplets comprised in the cosmetic or personal care composition and/or capture oil secreted by skin to prevent pooling of oil); binding (e.g., water binding, lipid binding, binding of bioactive agent); delivery (e.g., delivery of bioactive compound); oilcontrolling and/or oil removing (e.g., absorption or breaking up into droplets of excess oil
35
SUBSTITUTE SHEET ( RULE 26) produced and/or accumulated on body surface [e.g., to provide matte finish; to prevent acne, oily hair, or oily nail]); hypoallergenicity; softening of body surface; restructuring of body surface; glossing of body surface; de-glossing of body surface (e.g., to remove reflective shine); soothing of body surface (e.g., soothing of bum or sunbum); penetration of body surface (e.g., penetration into skin epidermis); revitalizing of body surface (e.g., modulate in situ production of collagen); activity on cutaneous necrosis; modulate (e.g., increase) rupture force of cicatrices; detangling of hair; strengthening, mending, and/or thickening of hair; humidity shielding (e.g., to enable long, sweat-free, and transfer-proof wear on body surface); encapsulating (e.g., to protect) active agent (e.g., any of the other ingredients disclosed herein); delivering active agent (e.g., any of the other ingredients disclosed herein); anti-microbial and/or anti-viral activity (e.g., by coating the surface of pathogenic organisms inhibiting adhesion and/or growth of microbes [e.g., Streptococci [e.g., Streptococcus mutans], Lactobacilli, Corynebacteria, Propionibacteria, Actinomycetes, Clostridia, Bacillus, Enterococcus, Staphylococci (e.g., Staphylococcus aureus), Enterobacteria, Escherichia, Salmonella, Shigella, Chlamydia, Candida albicans, Trichophyton species, Entamoeba histolytica, Giardia lamblia, and Cryptosporidium neoformatans] or viruses [e.g., Herpes viridae, Herpes simplex, Varicella-zoster, Epstein-barr, Poxviridae, (Orthopoxvirus and Avipoxvirus); Togaviridae, (Alphavirus, Flavivirus, Rubivirus and Pestivirus); Lyssavirus, California Encaphalitis Virus, Lassa Virus, Paramyxovirus, Pneumovirus and Morbillivirus]); anti-inflammatory activity ; activity on vascular permeability; and formulating (e.g., to increase content of an active agent [e.g., any of the other ingredients disclosed herein] that does not mix well in the medium of the composition).
[0106] The recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care composition according to any of the above may impart or materially contribute to such attribute of the cosmetic or personal care composition. The properties of milk proteins are in large part determined by the side chains of amino acids in the primary sequence of the milk proteins, the secondary and tertiary structures of the milk proteins, and on the interactions the secondary and tertiary structures permit the side chains to have with other molecules (e.g., other milk proteins, other non-milk proteins). Although the primary sequence of milk proteins can be maintained in their recombinant production, amino acid side chains as well as secondary and tertiary structures may be impacted by protein folding pathways and non-native post-translational modifications (PTMs) that may occur in recombinant host cells. Without wishing to be bound by theory, the recombinant milk protein comprised in the milk protein component comprised in a cosmetic or personal care composition
36
SUBSTITUTE SHEET ( RULE 26) of any of the above may have a non-native PTM that impacts a functionality of the recombinant milk protein, such that the recombinant milk protein imparts or materially contributes to an attribute of the cosmetic or personal care composition according to any of the above that differs from that imparted by a corresponding native milk protein in a corresponding cosmetic or personal care composition. Moreover, use of a native milk protein in production of a cosmetic or personal care composition often entails use of milk protein preparations that typically comprise at least small amounts of other components present in milk (e.g., other proteins, carbohydrates, minerals, lipids), which may impact an attribute of the cosmetic or personal care composition that is imparted or materially contributed to by the native milk protein. In contrast, recombinant production of a milk protein provides for preparations that are substantially purer, and that may therefor impart or materially contribute to an attribute of a cosmetic or personal care composition according to any of the above in a measurably different manner. For example, a cosmetic or personal care composition according to any of the above may comprise a lower amount of the recombinant milk protein than is necessary for the corresponding native protein in the corresponding cosmetic or personal care composition to obtain a similar attribute that is imparted or materially contributed to by the milk protein; and/or a cosmetic or personal care composition according to any of the above may comprise a different salt content than the corresponding cosmetic or personal care composition without effect on an attribute that is imparted or materially contributed to by the milk protein (e.g., without the recombinant milk protein becoming insoluble and leaving visible residue upon application of the cosmetic or personal care product on a body surface); and/or a cosmetic or personal care composition according to any of the above may have improved oil-controlling and/or oil removing capacity compared to the corresponding cosmetic or personal care composition; and/or a cosmetic or personal care composition according to any of the above may have improved adhesion to a body surface compared to the corresponding cosmetic or personal care composition; and/or a cosmetic or personal care composition according to any of the above may have a greater opacity (or other attribute) while having a lower lipid content compared to the corresponding cosmetic or personal care composition; and/or a cosmetic or personal care composition according to any of the above may deliver an active agent (e.g., any of the other ingredients disclosed herein) or be more effective or efficient at delivering such active agent compared to the corresponding cosmetic or personal care composition.
37
SUBSTITUTE SHEET ( RULE 26) Method for Producing Cosmetic or Personal Care Composition
[0107] In various aspects, provided herein is a method for producing a cosmetic or personal care composition according to any of the above, wherein the method comprises the step of obtaining a recombinant milk protein.
[0108] The recombinant milk protein may be obtained using in vitro methods (e.g., using cell-free transcription and/or translation systems). Alternatively, the recombinant milk protein may be produced using a recombinant host cell. The recombinant host cell may be derived from any wildtype organism, including any bacterium, fungus (e.g., yeast, filamentous fungus), archaea, unicellular protista, animal, plant, algae, protozoan, and chromista, or from a genetic variant (e.g., mutant) thereof, as well as from any generally recognized as safe (GRAS) industrial host cell.
[0109] Non-limiting examples of suitable bacteria include firmicutes, cyanobacteria (bluegreen algae), oscillatoriophcideae, bacillales, lactobacillales, oscillatoriales, bacillaceae, lactobacillaceae, and members of any of the following genera, and derivatives and crosses thereof: Acinetobacter, Acetobacter (e.g., Acetobacter suboxydans, Acetobacter xylinum), Actinoplane (e.g., Actinoplane missouriensis), Arthrospira (e.g., Arthrospira platensis, Arthrospira maxima), Bacillus (e g., Bacillus cereus, Bacillus coagulans, Bacillus licheniformis, Bacillus stearothermophilus, Bacillus subtilis), Escherichia (e.g., Escherichia coli), Lactobacillus (e.g., Lactobacillus acidophilus, Lactobacillus bulgaricus), Lactococcus (e.g., Lactococcus lactis, Lactococcus lactis Lancefield Group N, Lactobacillus reuteri), Leuconostoc (e.g., Leuconostoc citrovorum, Leuconostoc dextranicum, Leuconostoc mesenteroides), Micrococcus (e.g., Micrococcus lysodeikticus), Rhodococcus (e.g., Rhodococcus opacus, Rhodococcus opacus strain PD630), Spirulina, Streptococcus (e.g., Streptococcus cremoris, Streptococcus lactis, Streptococcus lactis subspecies diacetylactis. Streptococcus thermophilus), Streptomyces (e.g., Streptomyces chattanoogensis, Streptomyces gnseus, Streptomyces natalensis, Streptomyces olivaceus, Streptomyces olivochromogenes, Streptomyces rubiginosus), Tetrahymena (e.g., Tetrahymena thermophile, Tetrahymena hegewischi, Tetrahymena hyperangularis, Tetrahymena malaccensis, Tetrahymena pigmentosa, Tetrahymena pyriformis, Tetrahymena vorax ), and Xanthomonas (e.g., Xanthomonas campestns).
[0110] Non-limiting examples of suitable yeast include members of any of the following genera, and derivatives and crosses thereof: Blastobotrys, Candida (e g., Candida albicans, Candida etchellsii, Candida guilliermondii, Candida humilis, Candida lipolytica, Candida orthopsilosis, Candida palmioleophila, Candida pseudotropicalis, Candida sp., Candida utilis,
38
SUBSTITUTE SHEET ( RULE 26) Candida versatilis), Cladosporium, Cryptococcus (e.g., Cryptococcus terricolus, Cryptococcus curvatus), Debaryomyces (e.g., Debaryomyces hansenii), Endomyces (e.g., Endomyces vemalis), Endomycopsis (e.g., Endomycopsis vemalis), Eremothecium (e.g., Eremothecium ashbyii), Hansenula (e.g., Hansenula sp., Hansenula polymorpha), Kluyveromyces (e.g., Kluyveromyces sp., Kluyveromyces lactis, Kluyveromyces marxianus var. lactis, Kluyveromyces marxianus, Kluyveromyces thermotolerans), Lipomyces (e.g., Lipomyces starkeyi, Lipomyecs lipofer), Ogataea (e.g., Ogataea minuta), Pichia (e.g., Pichia sp., Pichia pastoris, Pichia finlandica, Pichia trehalophila, Pichia koclamae, Pichia membranaefaciens, Pichia minuta, Pichia lindnen), Pichia opuntiae, Pichia thermotolerans, Pichia salictaria, Pichia guercuum, Pichia pijperi, Pichia stiptis, Pichia methanolica), Rhodosporidium (e.g., Rhodosporidium toruloides), Rhodotorula (e.g., Rhodotorula sp., Rhodotorula gracilis, Rhodotorula glutinis, Rhodotorula graminis), Saccharomyces (e.g., Saccharomyces sp., Saccharomyces bayanus, Saccharomyces beticus, Saccharomyces cerevisiae, Saccharomyces chevalieri, Saccharomyces diastaticus, Saccharomyces ellipsoideus, Saccharomyces exiguus, Saccharomyces florentinus, Saccharomyces fragilis, Saccharomyces pastorianus, Saccharomyces pombe, Saccharomyces sake, Saccharomyces uvarum), Sporobolomyces (e.g., Sporobolomyces roseus), Sporidiobolus (e.g., Sporidiobolus johnsonii, Sporidiobolus salmonicolor), Trichosporon (e.g., Trichosporon cacaoliposimilis, Trichosporon oleaginosus sp. nov., Trichosporon cacaoliposimilis sp. nov., Trichosporon gracile, Trichosporon dulcitum, Trichosporon jirovecii, Trichosporon insectorum), Xanthophyllomyces (e.g., Xanthophyllomyces dendrorhous), Yarrowia (e.g., Yarrowia lipolytica), and Zygosaccharomyces (e.g., Zygosaccharomyces rouxii).
[OHl] Non-limiting examples of suitable filamentous fungi include any holomorphic, teleomorphic, and anamorphic forms of fungi, including members of any of the following genera, and derivatives and crosses thereof: Acremonium (e.g., Acremonium alabamense), Aspergillus (e.g., Aspergillus aculeatus, Aspergillus awamori, Aspergillus clavatus, Aspergillus flavus, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus niger var. awamori, Aspergillus ochraceus, Aspergillus oryzae, Aspergillus sojae, Aspergillus terreus, as well as Emericella, Neosartorya, and Petromyces species), Aureobasidium, Canariomyces, Chaetomium, Chaetomidium, Corynascus, Chrysosporium (e.g., Chrysosporium botr oides. Chrysosporium carmichaeli, Chrysosporium crassitunicatum, Chrysosporium europae, Chrysosporium evolceannui, Chrysosporium farinicola, Chrysosporium fastidium, Chrysosporium filiforme, Chrysosporium georgiae, Chrysosporium globiferum,
39
SUBSTITUTE SHEET ( RULE 26) Chrysosporium globiferum var. articulatum, Chrysosporium globiferum var. niveum, Chrysosporium hirundo, Chrysosporium hispanicum, Chrysosporium holmii, Chrysosporium indicum, Chrysosporium iops, Chrysosporium keratinophilum, Chrysosporium kreiselii, Chrysosporium kuzurovianum, Chrysosporium lignorum. Chrysosporium obatum, Chrysosporium lucknowense, Chrysosporium lucknowense Garg 27K, Chrysosporium medium, Chrysosporium medium var. spissescens, Chrysosporium mephiticum, Chrysosporium merdarium, Chrysosporium merdarium var. roseum, Chrysosporium minor, Chrysosporium panmcola, Chrysosporium parvum, Chrysosporium parvum var. crescens, Chrysosporium pilosum, Chrysosporium pseudomerdarium, Chrysosporium pyriformis, Chrysosporium queenslandicum, Chrysosporium sigleri, Chrysosporium sulfureum, Chrysosporium synchronum, Chrysosporium tropicum, Chrysosporium undulatum, Chrysosporium vallenarense, Chrysosporium vespertilium, Chrysosporium zonatum), Coonemeria, Cunninghamella (e.g., Cunninghamella ehinulata), Dactylomyces, Emericella, Filibasidium, Fusarium (e.g., Fusarium moniliforme, Fusarium venenatum, Fusarium oxysporum, Fusarium graminearum, Fusarium proliferatum, Fusarium verticiollioides, Fusarium culmorum, Fusarium crookwellense, Fusarium poae, Fusarium sporotrichioides, Fusarium sambuccinum, Fusarium torulosum, as well as associated Gibberella teleomorphic forms thereof), Gibberella, Humicola, Hypocrea, Lentinula, Malbranchea(e.g., Malbranchea filamentosa), Magnaporthe, Malbranchium, Melanocarpus, Mortierella (e.g., Mortierella alpina 1S-4, Mortieralla isabelline, Mortierrla vinacea, Mortieralla vinaceae var. raffinoseutilizer), Mucor (e.g., Mucor miehei Cooney et Emerson (Rhizomucor miehei (Cooney & R. Emerson)) S chipper, Mucor pusillus Lindt, Mucor circinelloides Mucor mucedo), Mycehophthora (e.g., Myceliophthora thermophila), Myrothecium, Neocallimastix, Neurospora (e.g., Neurospora crassa), Paecilomyces, Penicillium (e.g., Penicillium chrysogenum, Pennicillium iilacinum, Penicillium roquefortii), Phenerochaete, Phlebia, Piromyces, Pythium, Rhizopus (e.g., Rhizopus niveus), Schizophyllum, Scytalidium, Sporotrichum (e.g., Sporotrichum cellulophilum), Stereum, Talaromyces, Thermoascus, Thermomyces, Thielavia (e.g., Thielavia terrestris), Tolypocladium, and Trichoderma (e.g., Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei, Trichoderma atroviride, Trichoderma virens, Trichoderma citrinoviride, Trichoderma viride).
[0112] Non-limiting examples of suitable plants include cycads, ginkgo biloba, conifers, cypress, junipers, thuja, cedarwood, pines, angelica, caraway, coriander, cumin, fennel, parsley, dill, dandelion, helichrysum, marigold, mugwort, safflower, camomile, lettuce,
40
SUBSTITUTE SHEET ( RULE 26) wormwood, calendula, citronella, sages, thyme, chia seed, mustard, olive, coffee, capsicum, eggplant, paprika, cranberry, kiwi, vegetables (e.g., carrot, celery), tagetes, tansy, tarragon, sunflower, wintergreen, basil, hyssop, lavender, lemon verbena, marjoram, melissa, patchouli, pennyroyal, peppermint, rosemary, sesame, spearmint, primroses, samara, pepper, pimento, potato, sweet potato, tomato, blueberry, nightshades, petunia, morning glory, lilac, jasmin, honeysuckle, snapdragon, psyllium, wormseed, buckwheat, amaranth, chard, quinoa, spinach, rhubarb, jojoba, cypselea, chlorella, marula, hazelnut, canola, kale, bok choy, rutabaga, frankincense, myrrh, elemi, hemp, pumpkin, squash, curcurbit, manioc, dalbergia, legume plants (e.g., alfalfa, lentils, beans, clovers, peas, fava coceira, frijole bola roja, frijole negro, lespedeza, licorice, lupin, mesquite, carob, soybean, peanut, tamarind, wisteria, cassia, chickpea/garbanzo, fenugreek, green pea, yellow pea, snow pea, lima bean, fava bean), geranium, flax, pomegranate, cotton, okra, neem, fig, mulberry, clove, eucalyptus, tea tree, niaouli, fruiting plants (e.g., apple, apricot, peach, plum, pear, nectarine), strawberry, blackberry, raspberry, cherry, prune, rose, tangerine, citrus (e.g., grapefruit, lemon, lime, orange, bitter orange, mandarin, tangerine), mango, citrus bergamot, buchu, grape, broccoli, brussels sprout, camelina, cauliflower, rape, rapeseed (canola), turnip, cabbage, cucumber, watermelon, honeydew melon, zucchini, birch, walnut, cassava, baobab, allspice, almond, breadfruit, sandalwood, macadamia, taro, tuberose, aloe vera, garlic, onion, shallot, vanilla, yucca, vetiver, galangal, barley, com, curcuma aromatica, ginger, lemon grass, oat, palm, pineapple, rice, rye, sorghum, tnticale, turmeric, yam, bamboo, barley, cajuput, canna, cardamom, maize, oat, wheat, cinnamon, sassafras, lindera benzoin, bay laurel, avocado, ylang- ylang, mace, nutmeg, moringa, horsetail, oregano, cilantro, chervil, chive, aggregate fruits, grain plants, herbal plants, leafy vegetables, non-grain legume plants, nut plants, succulent plants, land plants, water plants, delbergia, millets, drupes, schizocarps, flowering plants, nonflowering plants, cultured plants, wild plants, trees, shrubs, flowers, grasses, herbaceous plants, brushes, lianas, cacti, tropical plants, subtropical plants, temperate plants, moss (e.g., Physcomitrella patens), and derivatives and crosses thereof.
[0113] The recombinant milk protein may be purified (i.e., be substantially separated from chemicals (e.g., carbohydrates, lipids, ash, metabolites, signaling molecules, other proteins), cellular components (e.g., cell walls, membrane lipids, chromosomes), and cells (e.g., other cells in an organism) of the source material from which the recombinant milk protein originated) comprised in a source material [e.g., a plant material, a fermentation broth]). For example, the recombinant milk protein may be purified to a purity of greater than 30%, greater than 35%, greater than 40%, greater than 45%, greater than 50%, greater than 55%, greater
41
SUBSTITUTE SHEET ( RULE 26) than 60%, greater than 65%, greater than 70%, greater than 75%, greater than 80%, greater than 85%, greater than 90%, greater than 95%, greater than 97%, or greater than 99% relative to other components comprised in the source material, or to at least 2-fold, at least 3 -fold, at least 4-fold, at least 5-fold, at least 6-fold, at least 7-fold, at least 8-fold, at least 9-fold, or at least 10-fold greater abundancy relative to other components comprised in the source material, or to a purity of greater than 30%, greater than 35%, greater than 40%, greater than 45%, greater than 50%, greater than 55%, greater than 60%, greater than 65%, greater than 70%, greater than 75%, greater than 80%, greater than 85%, greater than 90%, greater than 95%, greater than 97%, or greater than 99% by weight.
[0114] The method may further comprise the step of polymerizing the recombinant milk protein. Methods for polymerizing milk proteins are disclosed, for example, in U.S. patent publication US20060057131, published March 16, 2006; PCT publication WO2019213155, published November 7, 2019; and PCT publication WO2022031941, published February 10, 2022.
[0115] The method may further comprise the step of producing a coacervate comprising the recombinant milk protein and a polysaccharide. Methods for producing a coacervate comprising a recombinant milk protein and a polysaccharide are known in the art.
[0116] A variety of recipes known in the art may be used to prepare cosmetic or personal care product. The recombinant milk may be used in such recipes in purified/isolated form or comprised in a fermentation broth to produce a cosmetic or personal care product according to any of the above.
Method for Beautifying or Hygienizmg
[0117] In various aspects, provided herein is a method for beautifying or hygienizing, wherein the method comprises the step of applying to a body surface a cosmetic or personal care composition according to any of the above.
EXAMPLES
[0118] The following examples are included to illustrate specific aspects of this disclosure. The techniques disclosed in the examples represent techniques discovered by the inventors to function well in the methods and processes of this disclosure; however, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific aspects that are disclosed and still obtain a like or similar result without departing from
42
SUBSTITUTE SHEET ( RULE 26) the spirit and scope of the disclosure. Therefore, all matter set forth or show n in the examples is to be interpreted as illustrative and not in a limiting sense.
Example 1 : Skin lightening activity of recombinant B-lactoglobulin in melanoderm skin model [0119] The skin lightening activity of recombinantly produced B-lactoglobulin (diluted to various concentrations in PBS) was evaluated using a MelanoDerm 24 tissue kit (MELB-300; MatTek, Ashland. MA). To each well of hanging top 12 well plates, 5 mL of EPI-100-NMM- 113 culture medium (pre-warmed at 30-37C) was added. The MEL-300B tissue was removed from packaging using sterile forceps and transferred into the 12 well hanging top plates. After overnight incubation at 37°C and 5% CO2, both the lid and the hanging top lid of the plates were removed, the inserts were carefully removed and blotted, and the medium was replenished. To each insert, 25 pL of test sample was added, and the plates were further incubated. Additional dosing was done every alternate day. On day 14, the tissues were rinsed with PBS and blotted, and then cut and transferred into 1.5 mL Eppendorf tubes. To each tube, 300 pL of solvable was added, and the tubes were incubated overnight at 65°C in a thermoshaker, before the samples were analyzed in a melanin assay. Spectrophotometric measurements were performed to assess the skin lightening efficacy. The positive control (PC) consisted of 2% Kojic acid in ultrapure water.
[0120] As shown in Table 1, the highest melanin concentration was detected in the negative control (NT), followed by various test concentrations of recombinant P-lactoglobulin. The positive control (PC) exhibited the most significant melanin reduction compared to NT and the test samples.
[0121] As show n in Table 2, the positive control (PC) exhibited the brightest skin (highest L* value). Lower concentrations of recombinant B-lactoglobulin (0.63mg/ml, 0.2mg/ml, and 0.06mg/ml) showed higher L*values, indicating lighter skin. At even higher concentrations (20mg/ml, 6.33mg/ml, and 2mg/ml), the recombinant B-lactoglobulin showed lower L*values, suggesting less effective skin lightening.
Table 1: Melanin Estimation
Sample Concentration Melanin Melanin (%)
Figure imgf000044_0001
(pg /ml) Reduction
NT - 59.96 100.00 0.00
PC 2% Kojic 37.88 63.17 36.83
Al 20 mg/mL 58.10 96.90 3.10
A2 6.33 mg/mL 56.46 94.16 5.84
A3 2 mg/mL 56.67 94.51 5.49
A4 0.63 mg/mL 56.58 94.37 5.63
SUBSTITUTE SHEET ( RULE 26) A5 0.2 mg/mL 56.38 94.02 5.98
A6 0.06 mg/mL 56.44 94.13 5.87
Table 2: Spectrophotometric Values for Skin Tissue Concentrations
Sample Concentration L* Value
NT 44.29
PC 2% Kojic acid 46.04
Al 20 mg/mL 44.03
A2 6.33 mg/mL 44.11
A3 2 mg/mL 44.93
A4 0.63 mg/mL 45.48
A5 0.2 mg/mL 45.54
A6 0.06 mg/mL 45.60
Example 2: Anti-oxidant and anti-aging activity of recombinant B-lactoglobulin in epiderma skin model
[0122] The anti-oxidant and anti-aging activity of recombinantly produced P-lactoglobulin (diluted to various concentrations in PBS) was evaluated using a EpiDerm - Full Thickness 24 tissue kit (EFT-400; MatTek, Ashland, MA). To each well of 6 well plates, 5 mL of EFT-400- MM maintenance medium (pre-warmed to 20-25 °C) was added. The EFT-400 tissue was removed from packaging using sterile forceps and transferred into the 6 well plates. After overnight incubation at 37°C and 5% CO2, the inserts were carefully removed from the plates and blotted, and the media replenished. To each insert, 42 pL of test sample was added, and the plates were further incubated. Additional dosing was done every alternate day. On day 9, the tissues were rinsed with PBS and blotted, and then cut and transferred into 1.5 mL Eppendorf tubes. To each tube, 750 pL of cold lysis buffer with protease inhibitor cocktail was added. The tissues were homogenized with Pellet Pestle for approximately 30 seconds on ice, and the lysates were collected. Levels of analytes were determined by ELISA. The positive control (PC) consisted of ascorbic acid in ultrapure water.
[0123] As shown in Table 3, compared to NT, levels of glutathione peroxide (GPX), superoxide dismutase (SOD), and LPO were increased, and levels of prostaglandin were decreased, in the test samples, a molecular profile that is indicative of anti-oxidant activity.
[0124] As shown in Table 4, compared to NT, levels of COL3A1, COL1A1, hyaluronic acid, and filaggrin were increased in the test samples, a molecular profile that is indicative of anti-aging activity.
SUBSTITUTE SHEET ( RULE 26) Table 3: Anti-oxidant biomarkers release
Figure imgf000046_0001
Figure imgf000046_0002
45
SUBSTITUTE SHEET ( RULE 26)
Figure imgf000047_0001
SUBSTITUTE SHEET (RULE 26)

Claims

1. A cosmetic or personal care composition comprising a milk protein component, wherein the milk protein component comprises or consists essentially of a recombinant milk protein.
2. The cosmetic or personal care composition of claim 1 comprising between 0.1% and 100%, by mass of the milk protein component.
3. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is a liquid.
4. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is an emulsion.
5. The cosmetic or personal care composition of claim 4, wherein the emulsion comprises between 5% and 80% by mass of lipid.
6. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is a gel.
7. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is a powder.
8. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is an aerosol.
9. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is a semi-solid.
10. The cosmetic or personal care composition of claim 1 or 2, wherein the cosmetic or personal care composition is a solid.
11. The cosmetic or personal care composition of any of claims 1 through 10, wherein the cosmetic or personal care composition is essentially free of a component found in a mammal- produced milk, or comprises a lower concentration of at least one component found in a mammal-produced milk.
12. The cosmetic or personal care composition of any of claims 1 through 11, wherein the cosmetic or personal care composition is essentially free of a component obtained from an animal, or comprises 2% or less by mass of such component.
13. The cosmetic or personal care composition of any of claims 1 through 12, wherein the cosmetic or personal care composition is hypoallergenic.
14. The cosmetic or personal care composition of any of claims 1 through 13, wherein the recombinant milk protein is a single recombinant milk protein.
47
SUBSTITUTE SHEET ( RULE 26)
15. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant P-lactoglobulin.
16. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant a-lactalbumin.
17. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant GMP.
18. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant lactotransferrin.
19. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant bovine serum albumin.
20. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant lactoperoxidase.
21. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant K-casein.
22. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant P-casein.
23. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant a-Sl -casein.
24. The cosmetic or personal care composition of claim 14, wherein the single recombinant milk protein is a recombinant a-S2-casein.
25. The cosmetic or personal care composition of any of claims 1 through 13, wherein the recombinant milk protein is a mixture of two or more recombinant milk proteins.
26. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant P-lactoglobulin.
27. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant P- lactoglobulin and a recombinant a-lactalbumin.
28. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant P- lactoglobulin and a recombinant GMP.
29. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant P- lactoglobulin and a recombinant lactotransferrin.
48
SUBSTITUTE SHEET ( RULE 26)
30. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin and a recombinant bovine serum albumin.
31. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin and a recombinant lactoperoxidase.
32. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin and a recombinant K-casein.
33. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin and a recombinant 0-casein.
34. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin and a recombinant a-Sl-casein.
35. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin and a recombinant a-S2-casein.
36. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant GMP.
37. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant lactotransferrin.
38. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant bovine serum albumin.
39. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant lactoperoxidase.
40. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant K-casein.
49
SUBSTITUTE SHEET ( RULE 26)
41. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant 0-casein.
42. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant a-Sl-casein.
43. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-lactalbumin, and a recombinant a-S2-casein.
44. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant lactotransferrin.
45. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant bovine serum albumin.
46. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant lactoperoxidase.
47. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant K-casein.
48. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant 0-casein.
49. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant a-Sl -casein.
50. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant GMP, and a recombinant a-S2-casein.
51. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactotransferrin, and a recombinant bovine serum albumin.
50
SUBSTITUTE SHEET ( RULE 26)
52. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactotransferrin, and a recombinant lactoperoxidase.
53. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactotransferrin, and a recombinant K-casein.
54. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactotransferrin, and a recombinant 0-casein.
55. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactotransferrin, and a recombinant a-Sl -casein.
56. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactotransferrin, and a recombinant a-S2-casein.
57. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a bovine serum albumin, and a recombinant lactoperoxidase.
58. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant bovine serum albumin, and a recombinant K-casein.
59. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant bovine serum albumin, and a recombinant 0-casein.
60. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant bovine serum albumin, and a recombinant a-Sl -casein.
61. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant bovine serum albumin, and a recombinant a-S2-casein.
62. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactoperoxidase, and a recombinant K-casein.
51
SUBSTITUTE SHEET ( RULE 26)
63. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactoperoxidase, and a recombinant 0-casein.
64. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactoperoxidase, and a recombinant a-Sl -casein.
65. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant lactoperoxidase, and a recombinant a-S2-casein.
66. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant K-casein, and a recombinant 0-casein.
67. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant K-casein, and a recombinant a-Sl -casein.
68. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant K-casein, and a recombinant a-S2-casein.
69. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant 0-casein, and a recombinant a-Sl -casein.
70. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant 0-casein, and a recombinant a-S2-casein.
71. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0- lactoglobulin, a recombinant a-Sl -casein, and a recombinant a-S2-casein.
72. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant a-lactalbumin.
73. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin and a recombinant GMP.
52
SUBSTITUTE SHEET ( RULE 26)
74. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm and a recombinant lactotransferrin.
75. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin and a recombinant bovine serum albumin.
76. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin and a recombinant lactoperoxidase.
77. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm and a recombinant K-casein.
78. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm and a recombinant P-casein.
79. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactal bumin and a recombinant a-Sl -casein.
80. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalburmn and a recombinant a-S2-casein.
81. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant GMP, and a recombinant lactotransferrin.
82. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant GMP, and a recombinant bovine serum albumin.
83. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant GMP, and a recombinant lactoperoxidase.
84. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant GMP, and a recombinant K-casein.
53
SUBSTITUTE SHEET ( RULE 26)
85. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant GMP, and a recombinant 0-casein.
86. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactal bumin, a recombinant GMP, and a recombinant a-Sl -casein.
87. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant GMP, and a recombinant a-S2-casein.
88. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant lactotransferrin, and a recombinant bovine serum albumin.
89. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant lactotransferrin, and a recombinant lactoperoxidase.
90. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant lactotransferrin, and a recombinant K-casein.
91. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalburmn, a recombinant lactotransferrin, and a recombinant 0-casein.
92. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant lactotransferrin, and a recombinant a-Sl -casein.
93. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant lactotransferrin, and a recombinant a-S2-casein.
94. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant bovine serum albumin, and a recombinant lactoperoxidase.
95. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant bovine serum albumin, and a recombinant K-casein.
54
SUBSTITUTE SHEET ( RULE 26)
96. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant bovine serum albumin, and a recombinant P-casein.
97. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactal bumin, a recombinant bovine serum albumin, and a recombinant a-Sl -casein.
98. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant bovine serum albumin, and a recombinant a-S2-casein.
99. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant lactoperoxidase, and a recombinant K-casein.
100. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant lactoperoxidase, and a recombinant P-casein.
101. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactal bumin, a recombinant lactoperoxidase, and a recombinant a-Sl -casein.
102. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalburmn, a recombinant lactoperoxidase, and a recombinant a-S2-casein.
103. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant K-casein, and a recombinant P-casein.
104. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactal bumin, a recombinant K-casein, and a recombinant a-Sl -casein.
105. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumin, a recombinant K-casein, and a recombinant a-S2-casein.
106. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant P-casein, and a recombinant a-Sl -casein.
55
SUBSTITUTE SHEET ( RULE 26)
107. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactalbumm, a recombinant -casein, and a recombinant a-S2-casein.
108. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a- lactal bumin, a recombinant a-Sl -casein, and a recombinant a-S2-casein.
109. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant GMP.
110. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant lactotransferrin.
111. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant bovine serum albumin.
112. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant lactoperoxidase.
113. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant K-casein.
114. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant -casein.
115. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant a-Sl -casein.
116. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP and a recombinant a-S2-casein.
117. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactotransferrin, and a recombinant bovine serum albumin.
56
SUBSTITUTE SHEET ( RULE 26)
118. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactotransferrin, and a recombinant lactoperoxidase.
119. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactotransferrin, and a recombinant K-casein.
120. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactotransferrin, and a recombinant P-casein.
121. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactotransferrin, and a recombinant a-Sl -casein.
122. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactotransferrin, and a recombinant a-S2-casein.
123. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant bovine serum albumin, and a recombinant lactoperoxidase.
124. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant bovine serum albumin, and a recombinant K-casein.
125. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant bovine serum albumin, and a recombinant P-casein.
126. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant bovine serum albumin, and a recombinant a-Sl -casein.
127. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant bovine serum albumin, and a recombinant a-S2-casein.
128. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactoperoxidase, and a recombinant K-casein.
57
SUBSTITUTE SHEET ( RULE 26)
129. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactoperoxidase, and a recombinant P-casein.
130. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactoperoxidase, and a recombinant a-Sl -casein.
131. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant lactoperoxidase, and a recombinant a-S2-casein.
132. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant K-casem, and a recombinant P-casein.
133. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant K-casem, and a recombinant a-Sl -casein.
134. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant K-casein, and a recombinant a-S2-casein.
135. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant P-casein, and a recombinant a-Sl -casein.
136. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant P-casem, and a recombinant a-S2-casein.
137. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant GMP, a recombinant a-Sl -casein, and recombinant a-S2-casein.
138. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant lactotransferrin.
139. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin and a recombinant bovine serum albumin.
58
SUBSTITUTE SHEET ( RULE 26)
140. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin and a recombinant lactoperoxidase.
141. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin and a recombinant K-casein.
142. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin and a recombinant P-casein.
143. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin and a recombinant a-Sl -casein.
144. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin and a recombinant a-S2-casein.
145. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant lactoperoxidase.
146. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant K-casein.
147. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant P-casein.
148. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant a-S 1 -casein.
149. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant bovine serum albumin, and a recombinant a-S2-casein.
150. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant K-casein.
59
SUBSTITUTE SHEET ( RULE 26)
151. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant P-casein.
152. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant a-Sl -casein.
153. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant lactoperoxidase, and a recombinant a-S2-casein.
154. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant K-casein, and a recombinant P-casein.
155. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant K-casein, and a recombinant a-Sl -casein.
156. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant K-casein, and a recombinant a-S2-casein.
157. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant P-casein, and a recombinant a-Sl -casein.
158. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant P-casein, and a recombinant a-S2-casein.
159. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactotransferrin, a recombinant a-Sl -casein, and a recombinant a-S2-casein.
160. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant bovine serum albumin.
161. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin and a recombinant lactoperoxidase.
60
SUBSTITUTE SHEET ( RULE 26)
162. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin and a recombinant K-casein.
163. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin and a recombinant P-casein.
164. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin and a recombinant a-Sl -casein.
165. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin and a recombinant a-S2-casein.
166. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant K-casein.
167. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant p-casein.
168. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant a-Sl -casein.
169. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant lactoperoxidase, and a recombinant a-S2-casein.
170. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant K-casein, and a recombinant P-casein.
171. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant K-casein, and a recombinant a-Sl -casein.
172. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant K-casein, and a recombinant a-S2-casein.
61
SUBSTITUTE SHEET ( RULE 26)
173. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant P-casein, and a recombinant a-Sl -casein.
174. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant P-casein, and a recombinant a-S2-casein.
175. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant bovine serum albumin, a recombinant a-Sl-casein, and a recombinant a-S2-casein.
176. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant lactoperoxidase.
177. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase and a recombinant K-casein.
178. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase and a recombinant P-casein.
179. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase and a recombinant a-Sl -casein.
180. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase and a recombinant a-S2-casein.
181. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase, a recombinant K-casein, and a recombinant P-casein.
182. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase, a recombinant K-casein, and a recombinant a-Sl -casein.
183. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase, a recombinant K-casein, and a recombinant a-S2-casein.
62
SUBSTITUTE SHEET ( RULE 26)
184. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase, a recombinant P-casein, and a recombinant a-Sl -casein.
185. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase, a recombinant P-casein, and a recombinant a-S2-casein.
186. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant lactoperoxidase, a recombinant a-Sl -casein, and a recombinant a-S2-casein.
187. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant K-casein.
188. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant K-casein and a recombinant P-casein.
189. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant K-casein and a recombinant a-Sl -casein.
190. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant K-casein and a recombinant a-S2-casein.
191. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant K-casein, a recombinant P-casem, and a recombinant a-Sl -casein.
192. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant K-casein, a recombinant P-casem, and a recombinant a-S2-casein.
193. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of recombinant K-casein, a recombinant a-Sl -casein, and recombinant a-S2-casein.
194. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant P-casein.
195. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant P-casein and a recombinant a-Sl -casein.
63
SUBSTITUTE SHEET ( RULE 26)
196. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant -casein and a recombinant a-S2-casein.
197. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant 0-casein, a recombinant a-Sl -casein, and recombinant a-S2-casein.
198. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant a-Sl -casein.
199. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises or consists essentially of a recombinant a-Sl -casein and a recombinant a-S2-casein.
200. The cosmetic or personal care composition of claim 25, wherein the mixture of two or more recombinant milk proteins comprises a recombinant a-S2-casein.
201. The cosmetic or personal care composition of any of claims 1 through 200, wherein the recombinant milk protein is produced using a recombinant host cell.
202. The cosmetic or personal care composition of claim 201 , wherein the recombinant host cell is a recombinant bacterial host cell.
203. The cosmetic or personal care composition of claim 201 , wherein the recombinant host cell is a recombinant plant host cell.
204. The cosmetic or personal care composition of claim 201 , wherein the recombinant host cell is a recombinant fungal host cell.
205. The cosmetic or personal care composition of claim 204, wherein the recombinant fungal host cell is a recombinant yeast.
206. The cosmetic or personal care composition of claim 204, wherein the recombinant fungal host cell is a recombinant filamentous fungal host cell.
207. The cosmetic or personal care composition of claim 204, wherein the recombinant fungal host cell is derived from Saccharomyces, Kluyveromyces, Pichia, Tetrahymena, Yarrowia, Hansenula, Blastobotrys, Zygosaccharomyces, Debaryomyces, Aspergillus, Candida, Trichoderma, Fusarium, Chrysosporium, Physcomitrella, or Neurospora.
208. The cosmetic or personal care composition of any of claims 1 through 207, wherein the milk protein component comprises or consists essentially of a multimer or polymer of recombinant milk protein monomers.
64
SUBSTITUTE SHEET ( RULE 26)
209. The cosmetic or personal care composition of any of claims 1 through 208, wherein the milk protein component consists of whey protein and casein at a mass ratio of between about 10 to 0 and about 0 to 10.
210. The cosmetic or personal care composition of any of claims 1 through 209, wherein the milk protein component is comprised in a cosmetically or physiologically acceptable medium.
211. The cosmetic or personal care composition of any of claims 1 through 210, wherein the milk protein component is comprised in a coating layer around a core of the cosmetic or personal care composition.
212. The cosmetic or personal care composition of claim 211, wherein the core comprises an active agent.
213. The cosmetic or personal care composition of any of claims 1 through 212, wherein the recombinant milk protein is undenatured.
214. The cosmetic or personal care composition of any of claims 1 through 213, wherein the recombinant milk protein is hydrolyzed.
215. The cosmetic or personal care composition of claim 214, wherein the hydrolyzed recombinant milk protein consists essentially of peptides having a molecular weight of between 200 to 5,000.
216. The cosmetic or personal care composition of any of claims 1 through 215, wherein the recombinant milk protein is covalently or non-covalently associated with a lipid to form an amphiphilic complex.
217. The cosmetic or personal care composition of claim 216, wherein the lipid is selected from the group consisting of fatty acids or acyls having a carbon atom number of between 4 and 30.
218. The cosmetic or personal care composition of claim 216, wherein the lipid is selected from the group consisting of heptanoic acid, octanoic acid, decanoic acid, lauric acid, myristic acid, palmitic acid, stearic acid, ricinoleic acid, oleic acid, linoleic acid, linolenic acid, caprylic acid, capric acid; corresponding fatty alcohols and fatty amines; derivatives of said fatty acids, fatty alcohols, and fatty amines; and mixtures thereof.
219. The cosmetic or personal care composition of any of claims 216 through 218, wherein the recombinant milk protein comprises such lipid at a mass ratio of between about 1,000 to 1 and about 1 to 10.
220. The cosmetic or personal care composition of any of claims 216 through 219, wherein the lipid is covalently linked to the recombinant milk protein via a monofunctional agent or a multifunctional grafting agent.
65
SUBSTITUTE SHEET ( RULE 26)
221. The cosmetic or personal care composition of any of claims 1 through 220, wherein the recombinant milk protein is covalently or non-covalently associated with a coloring agent.
222. The cosmetic or personal care composition of claim 222, wherein the recombinant milk protein is covalently or non-covalently associated with a UVA and/or UVB light filtering or UVA and/or UVB light scattering agent.
223. The cosmetic or personal care composition of any of claims 1 through 222, wherein the recombinant milk protein is comprised in a coacervate with a polysaccharide.
224. A method for producing a cosmetic or personal care composition of any of claims 1 through 223, wherein the method comprises: obtaining a recombinant milk protein.
225. The method of claim 224, wherein the recombinant milk protein is produced using a recombinant host cell.
226. The method of claim 225, wherein the recombinant host cell is a recombinant bacterial host cell.
227. The method of claim 225, wherein the recombinant host cell is a recombinant plant host cell.
228. The method of claim 225, wherein the recombinant host cell is a recombinant fungal host cell.
229. The method of claim 228, wherein the recombinant fungal host cell is a recombinant yeast.
230. The method of claim 228, wherein the recombinant fungal host cell is a recombinant filamentous fungal host cell.
231. The method of claim 228, wherein the recombinant fungal host cell is derived from Saccharomyces, Kluyveromyces, Pichia, Tetrahymena, Yarrowia, Hansenula, Blastobotrys, Zygosaccharomyces, Debaryomyces, Aspergillus, Candida, Trichoderma, Fusarium, Chrysosporium, Physcomitrella, orNeurospora.
232. The method of any of claims 224 through 231, wherein the method further comprises polymerizing the recombinant milk protein.
233. The method of any of claims 224 through 232, wherein the method further comprises producing a coacervate comprising the recombinant milk protein and a polysaccharide.
234. A method for beautifying or hygienizing, wherein the method comprises: applying to a body surface a cosmetic or personal care composition of any of claims 1 through 223.
66
SUBSTITUTE SHEET ( RULE 26)
PCT/US2023/070793 2022-07-22 2023-07-23 Cosmetic or personal care composition comprising recombinant milk protein WO2024020592A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263391511P 2022-07-22 2022-07-22
US63/391,511 2022-07-22

Publications (1)

Publication Number Publication Date
WO2024020592A1 true WO2024020592A1 (en) 2024-01-25

Family

ID=89618540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2023/070793 WO2024020592A1 (en) 2022-07-22 2023-07-23 Cosmetic or personal care composition comprising recombinant milk protein

Country Status (1)

Country Link
WO (1) WO2024020592A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040170590A1 (en) * 2003-02-20 2004-09-02 Fahnestock Stephen R. Water-soluble silk proteins in compositions for skin care, hair care or hair coloring
WO2020219596A1 (en) * 2019-04-22 2020-10-29 Perfect Day, Inc. Recombinant milk proteins and compositions comprising the same
WO2022031941A1 (en) * 2020-08-05 2022-02-10 Perfect Day, Inc. Fusion proteins comprising milk proteins and compositions thereof
WO2022104227A1 (en) * 2020-11-13 2022-05-19 Perfect Day, Inc. Methods and compositions for producing recombinant components for use in food and other products

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040170590A1 (en) * 2003-02-20 2004-09-02 Fahnestock Stephen R. Water-soluble silk proteins in compositions for skin care, hair care or hair coloring
WO2020219596A1 (en) * 2019-04-22 2020-10-29 Perfect Day, Inc. Recombinant milk proteins and compositions comprising the same
WO2022031941A1 (en) * 2020-08-05 2022-02-10 Perfect Day, Inc. Fusion proteins comprising milk proteins and compositions thereof
WO2022104227A1 (en) * 2020-11-13 2022-05-19 Perfect Day, Inc. Methods and compositions for producing recombinant components for use in food and other products

Similar Documents

Publication Publication Date Title
CA1322532C (en) Trehalose containing cosmetic composition and method of using it
ES2198662T3 (en) USE OF RETINOIDS AS AN INDUCTIVE AGENT OF SKIN PIGMENTATION.
JPWO2016067688A1 (en) Skin barrier function improving agent and skin barrier function improving composition
JP2008538769A (en) Personal care compositions containing dipeptides
JP4252460B2 (en) Skin preparation for external use
WO2002072040A1 (en) Comsetics or external preparaiotns for skin
US11116708B2 (en) Personal care compositions including naringin:zinc complexes and methods for the same
JP5550863B2 (en) Cosmetics
JP2000309521A (en) Skin lotion
JP7044229B2 (en) Cellular β-catenin production promoting composition
JP4970805B2 (en) Topical skin preparation
KR102486936B1 (en) Cosmetics compositions for skin whitening
JP2008195651A (en) Humectant
WO2024020592A1 (en) Cosmetic or personal care composition comprising recombinant milk protein
JPH02111714A (en) Cosmetic
JP2009274968A (en) Hairdye composition containing protein nanoparticles
JP6173362B2 (en) Skin barrier function improving agent, intercellular adhesion structure formation promoter, tight junction formation promoter, TRPV4 activator, intracellular Ca concentration increase promoter and sebum production promoter
KR102083325B1 (en) Cosmetics compositions for skin whitening
KR102502511B1 (en) Cosmetics compositions for prevention and improvement of wrinkle
JP2011042613A (en) Anti-aging skin care preparation and anti-aging cosmetic
US10406086B2 (en) Moisturizer and cosmetic including the same
KR102491995B1 (en) Cosmetics compositions for skin whitening
KR102006951B1 (en) Cosmetics compositions for prevention and improvement of dandruff or itching
CN105963166A (en) Lipstick prepared form lycium ruthenicum mill
KR102491996B1 (en) Cosmetics compositions for prevention and improvement of wrinkle

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 23843953

Country of ref document: EP

Kind code of ref document: A1