WO2023282381A1 - Anti-photoaging composition comprising agastache rugosa extract and collagen peptide - Google Patents

Anti-photoaging composition comprising agastache rugosa extract and collagen peptide Download PDF

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WO2023282381A1
WO2023282381A1 PCT/KR2021/009902 KR2021009902W WO2023282381A1 WO 2023282381 A1 WO2023282381 A1 WO 2023282381A1 KR 2021009902 W KR2021009902 W KR 2021009902W WO 2023282381 A1 WO2023282381 A1 WO 2023282381A1
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composition
extract
photoaging
collagen
present
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PCT/KR2021/009902
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French (fr)
Korean (ko)
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유진희
최수영
윤원일
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코스맥스엔비티 주식회사
코스맥스엔에스 주식회사
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Publication of WO2023282381A1 publication Critical patent/WO2023282381A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

Definitions

  • the present invention relates to a composition capable of suppressing, preventing, and improving skin aging caused by light by including an extract of baechohyang and collagen peptides as active ingredients.
  • Sunlight consists of wavelengths divided into ultraviolet, visible, and infrared rays. Among them, ultraviolet rays are known to cause skin cancer and skin aging due to their short wavelength and strong energy.
  • Ultraviolet rays are invisible rays that penetrate deep into the skin and damage the dermis, causing skin aging, burns, and skin cancer. Ultraviolet rays are classified into UVA, UVB, and UVC according to the length of the wavelength, and among them, ultraviolet A (UVA) accounts for most of the ultraviolet rays reaching the ground because it has a relatively long wavelength and penetrates the atmosphere well. Ultraviolet A (UVA) is known to cause skin aging and skin irritation by penetrating the skin and promoting wrinkles and melanin formation.
  • UVB Ultraviolet B
  • Skin aging caused by continuous exposure to ultraviolet rays is called photoaging, and the most prominent symptoms of photoaging are wrinkles, spots, pigmentation, etc., and the degree of symptoms is proportional to the time and intensity of exposure to ultraviolet rays.
  • Lipids, proteins, polysaccharides, nucleic acids, etc. which are major components of the skin exposed to ultraviolet rays, are damaged and cells and tissues are destroyed, resulting in skin aging.
  • collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin which are skin connective tissues, are cut, which interferes with skin elasticity and forms wrinkles, which are the main symptoms due to photoaging. And, if it is more severe, it can cause mutations, cancer induction and immune function deterioration due to DNA mutation.
  • MMPs matrix metalloproteinases
  • An object of the present invention is to provide a composition exhibiting a synergistic effect of anti-aging of the skin due to the inclusion of baechohyang extract and collagen peptides as active ingredients.
  • the present invention provides a composition for anti-photoaging comprising a baechohyang extract and collagen peptides.
  • the weight ratio of the extract of baechohyang extract:collagen peptides may be 1:4 to 4:1.
  • the molecular weight of the collagen peptide may be Mw 1 to 2000.
  • step 2 filtering and concentrating the extract of step 1);
  • the extraction of step 1) includes hot water extraction
  • the step of filtering and concentrating in step 2) includes the step of vacuum filtration and rotary concentration
  • Preparing the powder of step 3) may include freeze drying or spray drying.
  • step 4) may include mixing the powder and the collagen peptides in a weight ratio of 1:4 to 4:1.
  • a food composition for anti-photoaging comprising the composition as described above is provided.
  • a cosmetic composition for anti-photoaging comprising the composition as described above is provided.
  • the anti-photoaging composition containing the extract and collagen peptides of the present invention the effect of suppressing, preventing and improving skin aging caused by light can be increased.
  • irritation to the skin can be minimized due to the inclusion of substances extracted from natural products.
  • 1 is a graph showing the collagen content.
  • 2 is a graph showing the content of MMP-1.
  • the present invention provides a composition for anti-photoaging comprising an extract of baechohyang and collagen peptides.
  • Baechohyang ( Agastache rugose ) is a perennial herb of the Lamiaceae family and is a plant with a unique scent.
  • Baechohyang contains fructose, glucose, sucrose, maltose, etc. as free sugar components, and glutamic acid, aspartic acid, leucine ( leucine) and the like, and includes potassium, calcium, sodium, magnesium, etc. as minerals, and includes ascorbic acid, polyphenols, and the like.
  • Water infused with baechohyang is also used as an external agent for skin diseases such as scabies or ringworm.
  • the collagen peptide of the present invention may be derived from plants or animals. Specifically, for example, it may include collagen peptides derived from fruits, vegetables, seeds, nuts, mushrooms, animal skin or bone, seaweed, fish and shellfish, fish, and the like.
  • Collagen peptides can be broken down into small molecules for easy absorption.
  • a decomposition method an enzymatic decomposition reaction, a hydrolysis reaction, or the like can be used.
  • the molecular weight of the collagen peptide may be Mw 1 to 2000, for example Mw 1 to 1000.
  • the weight ratio of baechohyang extract:collagen peptides may be 1:4 to 4:1, for example 4:1, for example 3:2.
  • the weight ratio of the extract of baechohyang to collagen peptides may be greater than 1 and less than or equal to 5, for example, greater than 1 and less than or equal to 4.
  • step 2 filtering and concentrating the extract of step 1);
  • step 1) may include adding water 1 to 100 times, for example, 1 to 50 times the raw Baechohyang.
  • Water is not particularly limited as long as it can be used for food or cosmetics.
  • stirring may be performed at 50 to 120° C., for example, 80 to 110° C. for 1 to 6 hours, for example, 2 to 4 hours.
  • the active ingredients that exhibit the anti-aging effect contained in the mixture may be damaged, and if the temperature is too low, extraction may be inefficient.
  • Water may be used as the extraction solvent, and in addition to alcohol, for example, ethanol such as alcohol may be added or replaced.
  • step 2) may include filtering under reduced pressure with a filter paper.
  • a step of concentrating the filtered extract with a vacuum rotary concentrator to remove solvent components may be included.
  • step 3) may include freeze drying or spray drying.
  • Freeze-drying sublimates the solidified solvent contained in the drying object into water vapor by reducing the pressure in a state in which the drying object is frozen by rapidly reducing the temperature.
  • Spray drying is a method of spraying an object to be dried in hot air and drying it in an air stream in a state of fine water droplets.
  • an additive such as dextrin may be used to increase the drying intensity.
  • step 4) may include mixing the powder and the collagen peptides in a weight ratio of 1:4 to 4:1.
  • a step of mixing the weight ratio of the extract of Baechohyang to collagen peptides in a range of more than 1 to 5 or less, for example, more than 1 to 4 or less may be included.
  • a functional food composition for anti-photoaging comprising the anti-photoaging composition described above may be provided.
  • the form of the food composition may be prepared in the form of a powder, granule, pill, tablet, capsule, etc., and is not particularly limited as long as it is in the form of a general food or beverage.
  • Types of food compositions may include all foods in the conventional sense, for example, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, and dairy including ice cream.
  • Products, various soups, beverages, tea, drinks, alcoholic beverages, vitamin complexes, etc. may be included, but are not limited to the above description.
  • Beverages and beverages may include general flavoring agents or natural carbohydrates as additional components, like conventional beverages.
  • the natural carbohydrate may include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.
  • Sweeteners may include natural sweeteners such as thaumatin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.
  • the food composition of the present invention includes general nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal viscous agents, pH adjusters, stabilizers, preservatives, glycerin, alcohol,
  • One or more carbonation agents used in carbonated beverages may be included, and fruit flesh contained in natural fruit juice, fruit juice beverages, and vegetable beverages may be included.
  • a cosmetic composition for anti-photoaging comprising the composition as described above.
  • the cosmetic composition of the present invention is conventionally prepared in the art, and may be prepared in any formulation that is dermatologically applicable.
  • Dermatologically applicable is a composition that is relatively non-toxic and harmless to the target and can have an effective effect, and may include an external agent applied to the skin, and side effects resulting from the composition may reduce the effectiveness of the active ingredient. It may mean that the activity and physical properties of the active ingredients are not damaged without causing serious irritation to the target.
  • the dermatologically applicable cosmetic composition of the present invention includes, for example, solutions, suspensions, emulsions, emulsions, pastes, gels, packs, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, and powder foundations. , It may be formulated as emulsion foundation, wax foundation, spray and hair cosmetics, but is not limited to the above description.
  • the cosmetic composition of the present invention is skin lotion, skin softener, skin toner, astringent, lotion, gel, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, foundation, essence, ampoule , Nutritional essence, pack, soap, hair shampoo, foot shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, etc.
  • Baechohyang extract and collagen peptide complex were prepared at the weight ratios shown in Table 1.
  • Baechohyang extract was extracted by hot water extraction method. Specifically, water was added to the raw product of baechohyang, and heated to 100 ° C. to obtain an extract of baechohyang while stirring for 2 hours.
  • the extract was filtered under reduced pressure using filter paper, and the filtered extract was concentrated using a vacuum rotary evaporator to remove solvent components.
  • the concentrated extract was obtained in powder form by spray drying.
  • Collagen peptides were prepared by hydrolysis twice, and the molecular weight is Mw 300 to 1000.
  • Human skin fibroblasts were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS) and 1% antibiotics at 37° C. under 5% CO 2 environmental conditions. After removing the medium, the cells were irradiated with UVB at 15 mJ/cm 2 using a UV crosslinker CL-1000M to induce photoaging. After that, the medium containing the composition according to each Example and Comparative Example was treated and cultured for 24 hours.
  • FBS fetal bovine serum
  • Collagen content was measured using a Procollagen I alpha 1 ELISA kit according to the manufacturer's protocol. After the cell culture was completed, the culture medium was collected, dispensed with an antibody cocktail to an immuno-well plate, and reacted at room temperature for 1 hour. After washing three times with a wash buffer, a TMB solution was dispensed and reacted for 10 minutes under light-blocking conditions. After 10 minutes, stop solution was added to stop the reaction. Absorbance was measured at a wavelength of 450 nm using a VersaMax TM tunable microplate reader, and the results are shown in FIG. 1 .
  • Example 1 the content of collagen was significantly increased by 1.5 times and 1.8 times, respectively, compared to the case where the untreated cells were irradiated with UV light. In particular, the effect of increasing the collagen content was the highest in Example 1.
  • the content of collagen degrading enzyme-1 (matrix metalloproteinases-1, MMP-1), one of the enzymes that degrade collagen, was measured using the Human Pro-MMP-1 Quantikin ELISA kit according to the manufacturer's protocol. After the cell culture was completed, the culture medium was collected, dispensed with an antibody cocktail to an immuno-well plate, and reacted at room temperature for 2 hours. After washing three times with wash buffer, Pro-MMP-1 Conjugate was added and reacted at room temperature for 2 hours. After washing three times with a washing buffer, a substrate solution was dispensed and reacted for 20 minutes under conditions where light was blocked. After 20 minutes, stop solution was added to stop the reaction. Absorbance was measured at a wavelength of 540 nm using a VersaMaxTM tunable microplate reader, and the results are shown in FIG. 2 .
  • MMP-1 As shown in Figure 2, it was confirmed that the content of MMP-1 was further reduced when the composition according to the Example was treated compared to the Comparative Example. In particular, in Examples 1 and 2, MMP-1 was greatly reduced to 1/5 compared to the case where UV rays were irradiated to untreated cells.
  • the anti-photoaging composition according to the present invention increases the collagen content and the MMP-1 inhibitory effect.
  • composition of the present invention can maximize the skin improvement effect against photoaging.

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Abstract

The present invention relates to a composition capable of inhibiting, preventing, and ameliorating skin aging caused by light due to the inclusion of an Agastache rugosa extract and collagen peptide as active ingredients. The present invention can minimize irritation to the skin due to the inclusion of a material extracted from a natural product.

Description

배초향 추출물 및 콜라겐 펩타이드를 함유하는 항광노화 조성물Anti-photoaging composition containing baechohyang extract and collagen peptides
본 발명은 배초향 추출물 및 콜라겐 펩타이드를 유효성분으로 포함함으로 인하여 광에 의한 피부 노화를 억제, 예방 및 개선할 수 있는 조성물에 관한 것이다.The present invention relates to a composition capable of suppressing, preventing, and improving skin aging caused by light by including an extract of baechohyang and collagen peptides as active ingredients.
태양광은 자외선, 가시광선, 적외선으로 구분되는 파장으로 구성된다. 이 중, 자외선은 파장이 짧고 에너지가 강하여 피부암 및 피부노화의 원인으로 알려져 있다.Sunlight consists of wavelengths divided into ultraviolet, visible, and infrared rays. Among them, ultraviolet rays are known to cause skin cancer and skin aging due to their short wavelength and strong energy.
자외선은 눈에 보이지 않는 광선으로 피부 속 깊숙하게 침투하여 진피를 손상시키며 피부노화, 화상, 피부암 등을 발생시키기도 한다. 자외선은 파장의 길이에 따라 UVA, UVB 및 UVC로 구분하며, 그 중 자외선 A(UVA)는 파장이 비교적 길어 대기를 잘 투과하기 때문에 지면에 도달하는 자외선의 대부분을 차지한다. 자외선 A(UVA)는 피부에 침투하여 주름과 멜라닌 형성을 촉진하는 등 피부 노화 및 피부 자극을 유발하는 것으로 알려져 있다.Ultraviolet rays are invisible rays that penetrate deep into the skin and damage the dermis, causing skin aging, burns, and skin cancer. Ultraviolet rays are classified into UVA, UVB, and UVC according to the length of the wavelength, and among them, ultraviolet A (UVA) accounts for most of the ultraviolet rays reaching the ground because it has a relatively long wavelength and penetrates the atmosphere well. Ultraviolet A (UVA) is known to cause skin aging and skin irritation by penetrating the skin and promoting wrinkles and melanin formation.
자외선 B(UVB)는 단시간 동안 과도하게 노출되면 피부의 표피를 침투하여 홍반, 주근깨 또는 부종 등을 유발한다. 자외선 B는 DNA와 결체조직에 손상을 주어 주로 광노화를 일으키는 주범이다.Ultraviolet B (UVB) rays penetrate the epidermis of the skin when exposed excessively for a short period of time, causing erythema, freckles, or swelling. UVB is the main cause of photoaging by damaging DNA and connective tissue.
자외선에 지속적으로 노출되어 생긴 피부노화를 광노화(photoaging)라 하며, 광노화의 가장 두드러진 증상은 주름, 반점, 색소침착 등이며, 증상의 정도는 자외선에 노출된 시간과 강도에 비례한다. 자외선에 노출된 피부는 주요 구성물질인 지질, 단백질, 다당류, 핵산 등이 손상되어 세포와 조직이 파괴되고, 결과적으로 피부의 노화가 발생한다. 특히, 피부 결합조직인 콜라겐(collagen), 히알루론산(hyaluronic acid), 엘라스틴(elastin), 프로테오글리칸(proteoglycan), 피브로넥틴(fibronectin) 등이 절단되어 피부 탄력에 지장을 주며 광노화로 인한 주요 증상인 주름을 형성하고, 더욱 심화할 경우 DNA 변이에 의해 돌연변이, 암 유발 및 면역기능 저하를 일으킬 수 있다.Skin aging caused by continuous exposure to ultraviolet rays is called photoaging, and the most prominent symptoms of photoaging are wrinkles, spots, pigmentation, etc., and the degree of symptoms is proportional to the time and intensity of exposure to ultraviolet rays. Lipids, proteins, polysaccharides, nucleic acids, etc., which are major components of the skin exposed to ultraviolet rays, are damaged and cells and tissues are destroyed, resulting in skin aging. In particular, collagen, hyaluronic acid, elastin, proteoglycan, and fibronectin, which are skin connective tissues, are cut, which interferes with skin elasticity and forms wrinkles, which are the main symptoms due to photoaging. And, if it is more severe, it can cause mutations, cancer induction and immune function deterioration due to DNA mutation.
생체 내에서는 평소에 콜라겐과 같은 세포 외 기질의 합성 및 분해가 적절하게 조절되는데, 광노화가 진행되면서, 특히, 자외선에 만성적으로 노출되는 부위에서는 콜라겐과 같은 세포 외 기질의 합성이 감소하고 분해가 촉진된다. 구체적으로, 자외선으로 인하여 콜라겐 분해 효소, 즉, 기질금속단백질 분해효소(matrix metalloproteinase, MMP)의 발현이 촉진되어 피부 탄력이 저하되고 주름이 형성된다.In vivo, the synthesis and degradation of extracellular matrix such as collagen are usually appropriately regulated. As photoaging progresses, especially in areas chronically exposed to ultraviolet rays, the synthesis and degradation of extracellular matrix such as collagen decreases and degradation is promoted. do. Specifically, expression of collagen degrading enzymes, that is, matrix metalloproteinases (MMPs), is promoted due to ultraviolet rays, thereby reducing skin elasticity and forming wrinkles.
또한, 최근에는 블루라이트(blue light) 영역의 가시광선으로 인한 광노화 역시 보고되고 있다.In addition, recently, photoaging due to visible light in the blue light region has also been reported.
이러한 광노화 현상을 예방, 억제, 개선 또는 치료하기 위한 유효성분을 포함하는 항광노화 조성물에 대한 연구가 이루어지고 있지만, 효과를 상승시키는 동시에 원재료의 가격으로 인한 경제적 문제, 피부 자극 또는 독성 문제 등의 단점을 해결하기에는 부족한 실정이다. 따라서, 세포독성을 유발하지 않으면서도 광에 의한 노화를 효과적으로 억제, 예방 및 개선하기 위한 조성물이 요구된다.Research on anti-photoaging compositions containing active ingredients for preventing, suppressing, improving or treating such photoaging phenomena has been conducted, but at the same time increasing the effect, economic problems due to the price of raw materials, skin irritation or toxicity problems, etc. is insufficient to solve the problem. Therefore, there is a need for a composition for effectively inhibiting, preventing, and improving photo-induced aging without causing cytotoxicity.
본 발명의 목적은 배초향 추출물 및 콜라겐 펩타이드를 유효성분으로 포함함으로 인하여 피부 항광노화의 상승효과를 나타내는 조성물을 제공하는 것이다.An object of the present invention is to provide a composition exhibiting a synergistic effect of anti-aging of the skin due to the inclusion of baechohyang extract and collagen peptides as active ingredients.
상기 과제를 해결하기 위하여, 본 발명은 배초향 추출물 및 콜라겐 펩타이드를 포함하는 항광노화용 조성물을 제공한다.In order to solve the above problems, the present invention provides a composition for anti-photoaging comprising a baechohyang extract and collagen peptides.
일 구현예에 따르면, 본 발명의 조성물은 배초향 추출물:콜라겐 펩타이드의 중량비가 1:4 내지 4:1일 수 있다.According to one embodiment, in the composition of the present invention, the weight ratio of the extract of baechohyang extract:collagen peptides may be 1:4 to 4:1.
일 구현예에 따르면, 상기 콜라겐 펩타이드의 분자량은 Mw 1 내지 2000일 수 있다.According to one embodiment, the molecular weight of the collagen peptide may be Mw 1 to 2000.
본 발명의 다른 구현예에 따르면 상기한 바와 같은 조성물을 제조하기 위하여,According to another embodiment of the present invention, in order to prepare the composition as described above,
1) 배초향에 물을 첨가하고, 가온하여 교반하면서 추출하는 단계;1) adding water to baechohyang, heating and extracting while stirring;
2) 상기 1)단계의 추출물을 여과 및 농축하는 단계;2) filtering and concentrating the extract of step 1);
3) 상기 2)단계의 농축물을 건조하여 파우더를 제조하는 단계; 및3) preparing a powder by drying the concentrate of step 2); and
4) 상기 3)단계의 파우더와 콜라겐 펩타이드를 혼합하는 단계를 포함하는, 항광노화용 조성물의 제조방법을 제공한다.4) It provides a method for preparing a composition for anti-aging, including mixing the powder of step 3) with collagen peptides.
일 구현예에 따르면, 상기 1)단계의 추출하는 단계는 열수추출하는 단계를 포함하고,According to one embodiment, the extraction of step 1) includes hot water extraction,
상기 2)단계의 여과 및 농축하는 단계는 감압여과 및 회전 농축하는 단계를 포함하고,The step of filtering and concentrating in step 2) includes the step of vacuum filtration and rotary concentration,
상기 3)단계의 파우더를 제조하는 단계는 동결 건조 또는 분무 건조하는 단계를 포함할 수 있다.Preparing the powder of step 3) may include freeze drying or spray drying.
일 구현예에 따르면, 상기 4)단계는 상기 파우더와 상기 콜라겐 펩타이드를 1:4 내지 4:1의 중량비로 혼합하는 단계를 포함할 수 있다.According to one embodiment, step 4) may include mixing the powder and the collagen peptides in a weight ratio of 1:4 to 4:1.
또한, 본 발명의 다른 구현예에 따르면, 상기한 바와 같은 조성물을 포함하는 항광노화용 식품 조성물을 제공한다.In addition, according to another embodiment of the present invention, a food composition for anti-photoaging comprising the composition as described above is provided.
본 발명의 또 다른 구현예에 따르면, 상기한 바와 같은 조성물을 포함하는 항광노화용 화장료 조성물을 제공한다.According to another embodiment of the present invention, a cosmetic composition for anti-photoaging comprising the composition as described above is provided.
기타 본 발명의 구현예들의 구체적인 사항은 이하의 상세한 설명에 포함되어 있다.Details of other embodiments of the present invention are included in the detailed description below.
본 발명의 배초향 추출물 및 콜라겐 펩타이드를 함유하는 항광노화 조성물에 따르면, 광에 의한 피부 노화를 억제, 예방 및 개선하는 효과를 상승시킬 수 있다. 또한, 천연물로부터 추출한 물질을 포함함으로 인하여 피부에 자극을 최소화할 수 있다.According to the anti-photoaging composition containing the extract and collagen peptides of the present invention, the effect of suppressing, preventing and improving skin aging caused by light can be increased. In addition, irritation to the skin can be minimized due to the inclusion of substances extracted from natural products.
도 1은 콜라겐 함량을 나타내는 그래프이다.1 is a graph showing the collagen content.
도 2는 MMP-1의 함량을 나타내는 그래프이다.2 is a graph showing the content of MMP-1.
본 발명은 다양한 변환을 가할 수 있고 여러 가지 실시예를 가질 수 있는 바, 특정 실시예를 도면에 예시하고 상세하게 설명하고자 한다. 그러나, 이는 본 발명을 특정한 실시 형태에 대해 한정하려는 것이 아니며, 본 발명의 사상 및 기술 범위에 포함되는 모든 변환, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다. 본 발명을 설명함에 있어서 관련된 공지 기술에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우 그 상세한 설명을 생략한다.Since the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail. However, it should be understood that this is not intended to limit the present invention to specific embodiments, and includes all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.
이하, 본 발명의 구현예에 따른 항광노화 조성물 및 그 제조방법에 대하여 보다 상세하게 설명한다.Hereinafter, an anti-aging composition according to an embodiment of the present invention and a manufacturing method thereof will be described in detail.
구체적으로 본 발명은 배초향 추출물 및 콜라겐 펩타이드를 포함하는 항광노화용 조성물을 제공한다.Specifically, the present invention provides a composition for anti-photoaging comprising an extract of baechohyang and collagen peptides.
배초향(Agastache rugose)은 꿀풀과의 다년생 초본으로, 독특한 향을 가지고 있는 식물이다. 배초향은 유리당 성분으로 프룩토스(fructose), 글루코스(glucose), 수크로스(sucrose), 말토스(maltose) 등을 포함하고, 아미노산으로 글루탐산(glutamic acid), 아스파트산(aspartic acid), 류신(leucine) 등을 포함하고, 무기질로 칼륨, 칼슘, 나트륨, 마그네슘 등을 포함하고, 아스코르브산(ascorbic acid), 폴리페놀류 등을 포함한다. 배초향을 달인 물은 신체의 옴이나 버짐과 같은 피부 질환에 외용제로서 사용되기도 한다. Baechohyang ( Agastache rugose ) is a perennial herb of the Lamiaceae family and is a plant with a unique scent. Baechohyang contains fructose, glucose, sucrose, maltose, etc. as free sugar components, and glutamic acid, aspartic acid, leucine ( leucine) and the like, and includes potassium, calcium, sodium, magnesium, etc. as minerals, and includes ascorbic acid, polyphenols, and the like. Water infused with baechohyang is also used as an external agent for skin diseases such as scabies or ringworm.
일 구현예에 따르면, 본 발명의 콜라겐 펩타이드는 식물 또는 동물로부터 유래된 것일 수 있다. 구체적으로 예를 들면, 과실류, 채소류, 종자류, 견과류, 버섯류, 동물의 피부 또는 골, 해조류, 어패류, 어류 등으로부터 유래하는 콜라겐 펩타이드를 포함할 수 있다.According to one embodiment, the collagen peptide of the present invention may be derived from plants or animals. Specifically, for example, it may include collagen peptides derived from fruits, vegetables, seeds, nuts, mushrooms, animal skin or bone, seaweed, fish and shellfish, fish, and the like.
콜라겐 펩타이드는 흡수가 용이하도록 저분자로 분해될 수 있다. 분해 방법으로는 효소 분해 반응, 가수분해 반응 등을 이용할 수 있다.Collagen peptides can be broken down into small molecules for easy absorption. As a decomposition method, an enzymatic decomposition reaction, a hydrolysis reaction, or the like can be used.
콜라겐 펩타이드의 분자량은 Mw 1 내지 2000, 예를 들면 Mw 1 내지 1000일 수 있다.The molecular weight of the collagen peptide may be Mw 1 to 2000, for example Mw 1 to 1000.
일 구현예에 따르면, 배초향 추출물:콜라겐 펩타이드의 중량비는 1:4 내지 4:1, 예를 들면 4:1, 예를 들면 3:2일 수 있다. 또한 예를 들면, 콜라겐 펩타이드에 대한 배초향 추출물의 중량비는 1 초과 5 이하, 예를 들면 1 초과 4 이하일 수 있다. 상기와 같은 중량비에서 항광노화 효과를 증대시킬 수 있으며, 콜라겐 펩타이드의 함량이 배초향 추출물에 비하여 높은 경우에는 콜라겐 증가 및 MMP-1 억제 효과가 충분하지 않을 수 있다.According to one embodiment, the weight ratio of baechohyang extract:collagen peptides may be 1:4 to 4:1, for example 4:1, for example 3:2. Also, for example, the weight ratio of the extract of baechohyang to collagen peptides may be greater than 1 and less than or equal to 5, for example, greater than 1 and less than or equal to 4. The anti-aging effect can be increased at the weight ratio as described above, and when the content of collagen peptides is higher than that of the extract of Baechohyang, the effect of increasing collagen and inhibiting MMP-1 may not be sufficient.
본 발명의 다른 구현예에 따르면,According to another embodiment of the present invention,
1) 배초향에 물을 첨가하고, 가온하여 교반하면서 추출하는 단계;1) adding water to baechohyang, heating and extracting while stirring;
2) 상기 1)단계의 추출물을 여과 및 농축하는 단계;2) filtering and concentrating the extract of step 1);
3) 상기 2)단계의 농축물을 건조하여 파우더를 제조하는 단계; 및3) preparing a powder by drying the concentrate of step 2); and
4) 상기 3)단계의 파우더와 콜라겐 펩타이드를 혼합하는 단계를 포함하는, 항광노화용 조성물의 제조방법을 제공한다.4) It provides a method for preparing a composition for anti-aging, including mixing the powder of step 3) with collagen peptides.
일 구현예에 따르면, 상기 1) 단계는 배초향 원물의 1 내지 100배수, 예를 들면 1 내지 50배수의 물을 첨가하는 단계를 포함할 수 있다. 물은 식품 또는 화장료에 사용할 수 있는 것이라면 특별히 제한하지 않는다. 물을 첨가한 후, 50 내지 120℃, 예를 들면 80 내지 110℃에서 1 내지 6시간, 예를 들면 2 내지 4시간 동안 교반하는 단계를 포함할 수 있다.According to one embodiment, step 1) may include adding water 1 to 100 times, for example, 1 to 50 times the raw Baechohyang. Water is not particularly limited as long as it can be used for food or cosmetics. After adding water, stirring may be performed at 50 to 120° C., for example, 80 to 110° C. for 1 to 6 hours, for example, 2 to 4 hours.
온도가 지나치게 상승하면 혼합물 내에 함유된 항광노화 효과를 나타내는 유효성분들이 손상될 수 있고, 온도가 지나치게 낮으면 추출이 비효율적일 수 있다. 추출 용매로는 물을 사용할 수 있고, 이외에 알코올, 예를 들면 주정과 같은 에탄올(ethanol)을 추가 또는 대체하여 사용할 수도 있다.If the temperature is too high, the active ingredients that exhibit the anti-aging effect contained in the mixture may be damaged, and if the temperature is too low, extraction may be inefficient. Water may be used as the extraction solvent, and in addition to alcohol, for example, ethanol such as alcohol may be added or replaced.
일 구현예에 따르면, 상기 2) 단계는 여과지로 감압 여과하는 단계를 포함할 수 있다. 또한, 여과된 추출물을 진공 회전 농축기로 농축하여 용매 성분을 제거하는 단계를 포함할 수 있다.According to one embodiment, step 2) may include filtering under reduced pressure with a filter paper. In addition, a step of concentrating the filtered extract with a vacuum rotary concentrator to remove solvent components may be included.
일 구현예에 따르면, 상기 3) 단계는 동결 건조 또는 분무 건조하는 단계를 포함할 수 있다. 동결건조는 온도를 급격하게 감소시켜 건조 대상을 얼린 상태에서 압력을 감소시킴으로 인하여 건조 대상이 포함하고 있는 고체화된 용매를 수증기로 승화시키는 것이다. 분무건조는 건조 대상을 열풍 속에 분무시켜 미세한 물방울 상태로 기류에 건조시키는 것이다. 예를 들면, 덱스트린(dectrin)과 같은 첨가제를 사용하여 건조의 강도를 증가시킬 수 있다.According to one embodiment, step 3) may include freeze drying or spray drying. Freeze-drying sublimates the solidified solvent contained in the drying object into water vapor by reducing the pressure in a state in which the drying object is frozen by rapidly reducing the temperature. Spray drying is a method of spraying an object to be dried in hot air and drying it in an air stream in a state of fine water droplets. For example, an additive such as dextrin may be used to increase the drying intensity.
일 구현예에 따르면, 상기 4)단계는 상기 파우더와 상기 콜라겐 펩타이드를 1:4 내지 4:1의 중량비로 혼합하는 단계를 포함할 수 있다. 또는, 콜라겐 펩타이드에 대한 배초향 추출물의 중량비를 1 초과 5 이하, 예를 들면 1 초과 4 이하로 혼합하는 단계를 포함할 수 있다.According to one embodiment, step 4) may include mixing the powder and the collagen peptides in a weight ratio of 1:4 to 4:1. Alternatively, a step of mixing the weight ratio of the extract of Baechohyang to collagen peptides in a range of more than 1 to 5 or less, for example, more than 1 to 4 or less may be included.
본 발명의 다른 구현예에 따르면, 상기한 바와 같은 항광노화 조성물을 포함하는 항광노화용 기능성 식품 조성물을 제공할 수 있다.According to another embodiment of the present invention, a functional food composition for anti-photoaging comprising the anti-photoaging composition described above may be provided.
식품 조성물의 형태로는 산제, 과립제, 환, 정제, 캡슐제의 형태 등으로 제조될 수 있고, 일반적인 식품 또는 음료의 형태라면 특별히 제한되지 않는다. 식품 조성물의 종류로는 통상적인 의미의 식품을 모두 포함할 수 있으며, 예를 들면, 육류, 소세지, 빵, 초콜렛, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올 음료, 비타민 복합제 등을 포함할 수 있으나, 상기한 기재에 한정되지는 않는다.The form of the food composition may be prepared in the form of a powder, granule, pill, tablet, capsule, etc., and is not particularly limited as long as it is in the form of a general food or beverage. Types of food compositions may include all foods in the conventional sense, for example, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, and dairy including ice cream. Products, various soups, beverages, tea, drinks, alcoholic beverages, vitamin complexes, etc. may be included, but are not limited to the above description.
음료수 및 음료는 통상의 음료와 같이 일반적인 향미제 또는 천연 탄수화물 등을 추가 성분으로 포함할 수 있다. 상기 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토스, 슈크로스와 같은 디사카라이드 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알코올을 포함할 수 있다. 감미제로는 타우마틴, 스테비아 추출물과 같은 천연 감미제, 사카린, 아스파르탐과 같은 합성 감미제 등을 포함할 수 있다.Beverages and beverages may include general flavoring agents or natural carbohydrates as additional components, like conventional beverages. The natural carbohydrate may include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. Sweeteners may include natural sweeteners such as thaumatin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.
또한, 본 발명의 식품 조성물은 일반적인 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 1종 이상 포함할 수 있고, 천연 과일쥬스, 과일쥬스 음료 및 야채 음료에 함유되는 과육을 포함할 수 있다.In addition, the food composition of the present invention includes general nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal viscous agents, pH adjusters, stabilizers, preservatives, glycerin, alcohol, One or more carbonation agents used in carbonated beverages may be included, and fruit flesh contained in natural fruit juice, fruit juice beverages, and vegetable beverages may be included.
본 발명의 또 다른 구현예에 따르면, 상기한 바와 같은 조성물을 포함하는 항광노화용 화장료 조성물을 제공할 수 있다.According to another embodiment of the present invention, it is possible to provide a cosmetic composition for anti-photoaging comprising the composition as described above.
본 발명의 화장료 조성물은 당업계에서 통상적으로 제조되며, 피부학적으로 적용이 가능한 어떠한 제형으로도 제조될 수 있다. 피부학적으로 적용이 가능하다는 것은 적용 대상에게 비교적 비독성이고 무해한 유효작용을 할 수 있는 조성물로서, 피부에 적용하는 외용제를 포함할 수 있으며, 상기 조성물로부터 기인하는 부작용이 유효 성분의 효능을 저하시키기 않고, 적용 대상에 심각한 자극을 유발하지 않으면서 유효성분들의 활성 및 물성을 손상시키지 않는다는 것을 의미할 수 있다.The cosmetic composition of the present invention is conventionally prepared in the art, and may be prepared in any formulation that is dermatologically applicable. Dermatologically applicable is a composition that is relatively non-toxic and harmless to the target and can have an effective effect, and may include an external agent applied to the skin, and side effects resulting from the composition may reduce the effectiveness of the active ingredient. It may mean that the activity and physical properties of the active ingredients are not damaged without causing serious irritation to the target.
본 발명의 피부학적으로 적용이 가능한 화장료 조성물로는 예를 들어, 용액, 현탁액, 유액, 유탁액, 페이스트, 겔, 팩, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션, 스프레이 및 모발 화장품 등으로 제형화될 수 있으나, 상기의 기재에 한정되는 것은 아니다. 구체적으로, 본 발명의 화장료 조성물은 스킨로션, 스킨소프너, 스킨토너, 아스트린젠트, 로션, 겔, 밀크로션, 모이스쳐 로션, 영양로션, 맛사지크림, 영양크림, 모이스처크림, 핸드크림, 파운데이션, 에센스, 엠플, 영양에센스, 팩, 비누, 헤어샴푸, 풋샴푸, 클렌징폼, 클렌징로션, 클렌징크림, 바디로션, 바디클린저 등의 제형 등으로 제조될 수 있다.The dermatologically applicable cosmetic composition of the present invention includes, for example, solutions, suspensions, emulsions, emulsions, pastes, gels, packs, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, and powder foundations. , It may be formulated as emulsion foundation, wax foundation, spray and hair cosmetics, but is not limited to the above description. Specifically, the cosmetic composition of the present invention is skin lotion, skin softener, skin toner, astringent, lotion, gel, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, moisture cream, hand cream, foundation, essence, ampoule , Nutritional essence, pack, soap, hair shampoo, foot shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, etc.
이하, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시예에 대하여 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다.Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.
실시예 및 비교예: 항광노화용 조성물의 제조Examples and Comparative Examples: Preparation of anti-aging composition
표 1과 같은 중량비로 배초향 추출물 및 콜라겐 펩타이드 복합물을 제조하였다.Baechohyang extract and collagen peptide complex were prepared at the weight ratios shown in Table 1.
배초향 추출물은 열수추출법으로 추출하였다. 구체적으로, 배초향 원물에 물을 첨가하고, 100℃로 가온하여 2시간 동안 교반하면서 배초향 추출물을 수득하였다.Baechohyang extract was extracted by hot water extraction method. Specifically, water was added to the raw product of baechohyang, and heated to 100 ° C. to obtain an extract of baechohyang while stirring for 2 hours.
여과지를 사용하여 상기 추출물을 감압 여과하고, 여과한 추출물을 진공 회전 농축기로 농축하여 용매 성분을 제거하였다. 농축한 추출물을 분무 건조하여 파우더 형태로 수득하였다.The extract was filtered under reduced pressure using filter paper, and the filtered extract was concentrated using a vacuum rotary evaporator to remove solvent components. The concentrated extract was obtained in powder form by spray drying.
콜라겐 펩타이드는 2번 가수분해하여 제조하였으며, 분자량은 Mw 300 내지 1000이다.Collagen peptides were prepared by hydrolysis twice, and the molecular weight is Mw 300 to 1000.
　 배초향 추출물Cabbage Fragrance Extract 저분자 콜라겐 펩타이드small molecule collagen peptides
비교예 1Comparative Example 1 55 00
실시예 1Example 1 44 1One
실시예 2Example 2 33 22
실시예 3Example 3 22 33
실시예 4Example 4 1One 44
비교예 2Comparative Example 2 00 55
실험예: 세포 배양 및 UVB 조사Experimental Example: Cell culture and UVB irradiation
인간 피부 섬유아세포를 10% 소 태아 혈청(fetal bovine serum, FBS)와 1% 항생제(antibiotics)가 함유된 Dulbecco's modified Eagle's medium로 37℃에서 5% CO2의 환경 조건으로 배양하였다. 배지를 제거한 후, UV crosslinker CL-1000M를 이용하여 UVB를 15 mJ/cm2로 세포에 조사하여 광노화를 유도하였다. 그 후, 각각의 실시예 및 비교예에 따른 조성물이 함유된 배지를 처리하여 24시간 동안 배양하였다.Human skin fibroblasts were cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (FBS) and 1% antibiotics at 37° C. under 5% CO 2 environmental conditions. After removing the medium, the cells were irradiated with UVB at 15 mJ/cm 2 using a UV crosslinker CL-1000M to induce photoaging. After that, the medium containing the composition according to each Example and Comparative Example was treated and cultured for 24 hours.
실험예 1: 콜라겐 함량 측정Experimental Example 1: Collagen content measurement
Procollagen I alpha 1 ELISA kit를 사용하여 제조사의 프로토콜(protocol)에 따라 콜라겐의 함량을 측정하였다. 세포 배양이 끝난 후, 배지를 회수하여 Immuno-well plate에 항체 칵테일(antibody cocktail)과 함께 분주하고 실온에서 1시간 동안 반응하였다. 세정 버퍼(wash buffer)로 3번 세척한 후, TMB 용액(TMB solution)을 분주하고 빛이 차단된 조건에서 10분간 반응하였다. 10분 후, 정지액(stop solution)을 넣어 반응을 중지시켰다. VersaMaxTM tunable microplate reader를 이용하여 450 nm의 파장에서 흡광도를 측정하였으며, 결과는 도 1에 도시하였다.Collagen content was measured using a Procollagen I alpha 1 ELISA kit according to the manufacturer's protocol. After the cell culture was completed, the culture medium was collected, dispensed with an antibody cocktail to an immuno-well plate, and reacted at room temperature for 1 hour. After washing three times with a wash buffer, a TMB solution was dispensed and reacted for 10 minutes under light-blocking conditions. After 10 minutes, stop solution was added to stop the reaction. Absorbance was measured at a wavelength of 450 nm using a VersaMax TM tunable microplate reader, and the results are shown in FIG. 1 .
도 1에 나타난 바와 같이, 비교예에 비하여 실시예에 따른 조성물을 처리한 경우 콜라겐의 함량이 더 높은 것을 확인하였다.As shown in Figure 1, it was confirmed that the collagen content was higher when the composition according to the Example was treated compared to the Comparative Example.
실시예 1 및 2는 아무것도 처리하지 않은 세포에 자외선을 조사한 경우에 비하여 콜라겐의 함량이 각각 1.5배, 1.8배로 크게 증가하였다. 특히, 콜라겐 함량 증가 효과는 실시예 1에서 가장 높게 나타났다.In Examples 1 and 2, the content of collagen was significantly increased by 1.5 times and 1.8 times, respectively, compared to the case where the untreated cells were irradiated with UV light. In particular, the effect of increasing the collagen content was the highest in Example 1.
실험예 2: MMP-1 함량 측정Experimental Example 2: MMP-1 content measurement
콜라겐을 분해하는 효소 중 하나인 콜라겐 분해 효소-1(matrix metalloproteinases-1, MMP-1)의 함량은 Human Pro-MMP-1 Quantikin ELISA kit를 사용하여 제조사의 프로토콜에 따라 측정하였다. 세포 배양이 끝난 후, 배지를 회수하여 Immuno-well plate에 항체 칵테일(antibody cocktail)과 함께 분주하고 실온에서 2시간 동안 반응하였다. 세정 버퍼(wash buffer)로 3번 세척 후, Pro-MMP-1 Conjugate를 첨가하고 실온에서 2시간 동안 반응하였다. 세정 버퍼로 3번 세척 후, 기질 용액(substrate solution)을 분주하고 빛이 차단된 조건에서 20분간 반응하였다. 20분 후, 정지액(stop solution)을 넣어 반응을 중지시켰다. VersaMaxTM tunable microplate reader를 이용하여 540 nm의 파장에서 흡광도를 측정하였으며, 결과는 도 2에 도시하였다.The content of collagen degrading enzyme-1 (matrix metalloproteinases-1, MMP-1), one of the enzymes that degrade collagen, was measured using the Human Pro-MMP-1 Quantikin ELISA kit according to the manufacturer's protocol. After the cell culture was completed, the culture medium was collected, dispensed with an antibody cocktail to an immuno-well plate, and reacted at room temperature for 2 hours. After washing three times with wash buffer, Pro-MMP-1 Conjugate was added and reacted at room temperature for 2 hours. After washing three times with a washing buffer, a substrate solution was dispensed and reacted for 20 minutes under conditions where light was blocked. After 20 minutes, stop solution was added to stop the reaction. Absorbance was measured at a wavelength of 540 nm using a VersaMaxTM tunable microplate reader, and the results are shown in FIG. 2 .
도 2에 나타난 바와 같이, 비교예에 비하여 실시예에 따른 조성물을 처리한 경우 MMP-1의 함량이 더 저하함을 확인하였다. 특히, 실시예 1 및 2는 아무것도 처리하지 않은 세포에 자외선을 조사한 경우에 비하여 MMP-1이 1/5로 크게 감소하였다.As shown in Figure 2, it was confirmed that the content of MMP-1 was further reduced when the composition according to the Example was treated compared to the Comparative Example. In particular, in Examples 1 and 2, MMP-1 was greatly reduced to 1/5 compared to the case where UV rays were irradiated to untreated cells.
상기와 같은 결과로부터 확인할 수 있는 바와 같이, 본 발명에 따른 항광노화 조성물은 콜라겐 함량 증가 및 MMP-1 억제 효과를 상승시킴이 확인된다.As can be confirmed from the above results, it is confirmed that the anti-photoaging composition according to the present invention increases the collagen content and the MMP-1 inhibitory effect.
따라서, 본 발명의 조성물은 광노화에 대한 피부 개선 효과를 극대화할 수 있다.Therefore, the composition of the present invention can maximize the skin improvement effect against photoaging.
이상의 설명은 본 발명의 기술 사상을 예시적으로 설명한 것에 불과한 것으로서, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 다양한 수정 및 변형이 가능하다. 또한, 본 발명에 개시된 실시 예들은 본 발명의 기술 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시 예에 의하여 본 발명의 기술 사상의 범위가 한정되는 것은 아니다. 본 발명의 보호 범위는 아래의 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The above description is merely an example of the technical idea of the present invention, and various modifications and variations can be made to those skilled in the art without departing from the essential characteristics of the present invention. In addition, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed according to the claims below, and all technical ideas within the equivalent range should be construed as being included in the scope of the present invention.

Claims (8)

  1. 배초향 추출물 및 콜라겐 펩타이드를 포함하는 항광노화용 조성물.An anti-aging composition comprising an extract of baechohyang and collagen peptides.
  2. 제1항에 있어서,According to claim 1,
    배초향 추출물:콜라겐 펩타이드의 중량비가 1:4 내지 4:1인 것인, 항광노화용 조성물.Baechohyang extract: The weight ratio of collagen peptides is 1:4 to 4:1, anti-aging composition.
  3. 제1항에 있어서,According to claim 1,
    상기 콜라겐 펩타이드의 분자량이 Mw 1 내지 2000인 것인, 항광노화용 조성물.The molecular weight of the collagen peptide is Mw 1 to 2000, anti-aging composition.
  4. 제1항에 따른 조성물을 제조하는 방법으로,A method for preparing the composition according to claim 1,
    1) 배초향에 물을 첨가하고, 가온하여 교반하면서 추출하는 단계;1) adding water to baechohyang, heating and extracting while stirring;
    2) 상기 1)단계의 추출물을 여과 및 농축하는 단계;2) filtering and concentrating the extract of step 1);
    3) 상기 2)단계의 농축물을 건조하여 파우더를 제조하는 단계; 및3) preparing a powder by drying the concentrate of step 2); and
    4) 상기 3)단계의 파우더와 콜라겐 펩타이드를 혼합하는 단계를 포함하는, 항광노화용 조성물의 제조방법.4) A method for preparing a composition for anti-photoaging comprising mixing the powder of step 3) with collagen peptides.
  5. 제4항에 있어서,According to claim 4,
    상기 1)단계의 추출하는 단계가 열수추출하는 단계를 포함하고,The extraction step of step 1) includes hot water extraction,
    상기 2)단계의 여과 및 농축하는 단계가 감압여과 및 회전 농축하는 단계를 포함하고,The step of filtering and concentrating in step 2) includes the step of vacuum filtration and rotary concentration,
    상기 3)단계의 파우더를 제조하는 단계가 동결 건조 또는 분무 건조하는 단계를 포함하는 것인, 항광노화용 조성물의 제조방법.Wherein the step of preparing the powder of step 3) comprises freeze-drying or spray-drying, a method for preparing an anti-aging composition.
  6. 제4항에 있어서,According to claim 4,
    상기 4)단계가 상기 파우더와 상기 콜라겐 펩타이드를 1:4 내지 4:1의 중량비로 혼합하는 단계를 포함하는 것인, 항광노화용 조성물의 제조방법.Wherein step 4) comprises mixing the powder and the collagen peptides in a weight ratio of 1:4 to 4:1, a method for preparing a composition for anti-photoaging.
  7. 제1항에 따른 조성물을 포함하는, 항광노화용 식품 조성물.A food composition for anti-aging, comprising the composition according to claim 1.
  8. 제1항에 따른 조성물을 포함하는, 항광노화용 화장료 조성물.A cosmetic composition for anti-aging, comprising the composition according to claim 1.
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