KR20200011243A - Composition for anti-oxidation, anti-inflammation, or anti-wrinkle comprising extract of green ball apple peel - Google Patents

Abstract

The present invention relates to an antioxidant, anti-inflammatory, or wrinkle-ameliorating composition including green ball apple peel extract as an active ingredient. The peel extract of green ball (Malus pumila Mill.) apples, which is a newly developed species, of the present invention has an antioxidant effect by inhibiting DPPH radical, ABTS radical, and TBARs and increasing PF and has an effect of suppressing an inflammatory response by inhibiting hyaluronidase activity. Moreover, the present invention is expected to be useful as an antioxidant, anti-inflammatory, or wrinkle-ameliorating pharmaceutical composition, a health functional food composition, and a cosmetic composition since elastin molecular morphology is maintained by inhibiting elastase activity and a wrinkle-ameliorating effect is provided by inhibiting collagenase activity.

Description

Composition for anti-oxidation, anti-oxidation, anti-inflammation, or anti-wrinkle comprising extract of green ball apple peel}

The present invention relates to an antioxidant, anti-inflammatory, or anti-wrinkle pharmaceutical composition, health functional food composition, or cosmetic composition comprising the greenball apple peel extract as an active ingredient.

One of Korea's representative fruits, the apple ( Malus pumila Mill.) As the income level improves due to economic growth, the form of consumption is changing to quality rather than quantity. Busa; Malus , commonly called 'adverb' domestica Borkh.) is the most representative apple variety, accounting for 29.5% of the total domestic fruit production as of 2012, and is mainly exported to Taiwan. About 50% of domestic apples are consumed, and the rest are stored and consumed in the following year. Fuji apples, which are exported after low-temperature storage, have been limited in export since February of the following year due to deterioration in quality. In addition, recognition and prices are much lower than Japanese apples, so Korean apples are still regarded as medium in quality. However, as consumer concerns about food safety increased after the nuclear accident in Japan in 2011, imports of Japanese agri-food from Taiwan, Korea, China, and the United States declined. It is becoming. In China's case, strict measures have been taken to prohibit the importation of agri-food in areas considered safe in Japan. After the nuclear accident in Japan, it was necessary to develop high-quality apples that can be exported to various countries, especially in China, by taking advantage of Korean food safety and price competitiveness.

Apples are a perennial herbaceous plant belonging to the taxonomic family Rosaceae, grown in the northern hemisphere of Europe, Asia, and North America. In the private world, apples are used for medicine, vomiting, hookworm, blood donation, and constipation. One type of apple, the tomb, manages beef, kwakran, abdominal pain, removes the wall, and controls dysentery. Unripe tortilla is tasteless but used as medicine. But if you eat a lot, you get a lot of sleep, you get a wall and you boil. Apple cider, made by fermenting apples, has a unique flavor, especially after digestion. If the cider is oxidized and fermented to make it more suitable for burns, local medicine says that the pain stops immediately, and if it is applied to the affected area day and night, the area is much smaller after one month. Apples have proven their effectiveness not only through folk remedies but also through research. Apple fruit is sensory and nutritious and contains bioactive substances such as vitamins, minerals, potassium, sodium and polyphenol compounds in addition to palates such as organic acids and sugars. Among them, various polyphenols such as procyanidin, chlorogenic acid, caffeic acid, epicatechin, catechin, p-coumaroyl qunic acid, rutin, phloridzin, quercetin are used for skin aging and obesity caused by oxidative damage of biological components by free radicals. It is known to be effective in preventing degenerative diseases such as inflammation, arteriosclerosis, diabetes, hypertension and various cancers (Yun HJ, Lim SY, Hur JM, Jeong JW, Yang SH, Kim DH. 2007. Korean J Food Preserv 14: 239-246.).

Among the various varieties of apples, Green ball; Malus pumila Mill.) Apples are new varieties of apples grown in Korea using Golden Delicious (Fubon) and Fuji (Fubon), which are ripe from late August to early September. It is 14 ° brix and weighs about 327g. Greenball apples are green-yellow apples that can be tasted at the end of the summer. The greenball apples are green, ripe and sour. It can also be cultivated in the southern regions with less labor and relatively high temperatures.

On the other hand, biologically active substances are widely distributed in animals and plants, and plants contain polyphenol-based antioxidant substances in cells to protect themselves from oxidative oxidation, automatic oxidation and damage caused by insects, and flavonoids. Acidic phenolic compounds are known to have various physiological activities such as antioxidant, anti-allergy, anti-diabetic, anti-cancer, anti-inflammatory, whitening, wrinkle improvement. Recently, many researches and developments have been made in the fields of functional foods and functional cosmetics for the physiological activity and excellent functional substances derived from medicinal plant resources, which have been proven effective, and search for substances in medicinal parts such as medicinal plants, wild vegetables, herbal medicines and fruits. Therefore, the development of health functional food or food supplementary materials is very meaningful in terms of efficient use of natural resources, new food resources development, and new materials development.

Thus, the present inventors attempted to develop a high value-added functional material by analyzing the effects of antioxidant, anti-inflammatory, and wrinkle improvement on the apples of the greenball variety, a new breed bred in Korea.

The present inventors confirmed that the apples of the new breed of greenball varieties bred in Korea had better antioxidant, anti-inflammatory, and anti-wrinkle effects than the adverb varieties, and found that the effect was the most remarkable in the peel extract of greenball apples. .

Accordingly, it is an object of the present invention to provide an antioxidant, anti-inflammatory, or anti-wrinkle pharmaceutical composition, health functional food composition, or cosmetic composition comprising the greenball apple peel extract as an active ingredient.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problem, another task not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, the present invention is an antioxidant for containing a green ball apple peel extract as an active ingredient; Anti-inflammatory; Or it provides a pharmaceutical composition for wrinkle improvement.

In addition, the present invention for the antioxidant containing a green ball apple peel extract as an active ingredient; Anti-inflammatory; Or it provides a functional food composition for improving wrinkles.

In addition, the present invention for the antioxidant containing a green ball apple peel extract as an active ingredient; Anti-inflammatory; Or to provide a cosmetic composition for improving wrinkles.

In one embodiment of the invention, the composition is 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical inhibition, 2,2'-azinobis- (3 Inhibition of 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid; ABTS) radicals, inhibition of Thiobarbituric acid reactive substances (TBARs), or It can increase the antioxidant protection factor (PF).

In another embodiment of the present invention, the composition may inhibit hyaluronidase.

In another embodiment of the present invention, the composition may inhibit elastase or collagenase.

In another embodiment of the present invention, the greenball apple peel extract is water, C ₁ to C ₄ lower alcohol, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, And it may be extracted with a solvent selected from the group consisting of mixed solvents thereof.

In addition, the present invention provides an antioxidant comprising administering the pharmaceutical composition to a subject; Anti-inflammatory; Or it provides a wrinkle improvement method.

In addition, the present invention provides antioxidants of the pharmaceutical composition; Anti-inflammatory; Or anti-wrinkle applications.

Green ball (Malus pumila Mill.) Apple peel extract of the new breed of the present invention has an antioxidant effect by inhibiting DPPH radicals, ABTS radicals, and TBARs and increasing PF, and inhibits hyaluronidase activity to inflame the inflammatory response. It shows an inhibitory effect. In addition, it inhibits elastase activity and maintains elastin molecular form, and inhibits collagenase activity, thereby showing an anti-wrinkle effect.

Therefore, the greenball apple peel extract according to the present invention is expected to be useful as an antioxidant, anti-inflammatory, or anti-wrinkle pharmaceutical composition, health food composition, and cosmetic composition.

1 is a view showing the phenolic content in the water and ethanol extract of the peel (peel) and whole apple of the greenball apple according to an embodiment of the present invention.
Figure 2 is a view showing the collagenase inhibitory effect in the peel and whole apple extract of the greenball apple according to an embodiment of the present invention.
Figure 3a is a view showing the results of measuring the DPPH radical scavenging ability by solvent (water, ethanol) for comparing the physiological activity of the solid and phenolic components of the greenball apple peel according to an embodiment of the present invention.
Figure 3b is a view showing the results of measuring the DPPH radical scavenging ability by solvent (water, ethanol) to compare the physiological activity of the solid and phenolic components of the green ball whole apple according to an embodiment of the present invention.
Figure 3c is a view showing the results of measuring the respective ABTS radical scavenging ability by solvent (water, ethanol) for comparing the physiological activity of the solid content and phenolic components of the greenball apple peel according to an embodiment of the present invention.
3d is a view showing the results of measuring the ABTS radical scavenging ability by solvent (water, ethanol) for comparing the physiological activity of the solid content and phenolic components of the green ball whole apple according to an embodiment of the present invention.
Figure 4a is a view showing the phenolic content in the solvent-specific extracts of the greenball apple peel according to an embodiment of the present invention.
Figure 4b is a view showing the phenolic content in the extract of ethanol concentration of greenball apple peel according to an embodiment of the present invention.
Figure 4c is a view showing the phenolic content in the ethanol concentration extract of greenball whole apples according to an embodiment of the present invention.
5 is a view showing the results of measuring DPPH radical scavenging ability according to phenolic concentrations in water and ethanol extracts of greenball apple peel and adverb apple peel according to one embodiment of the present invention.
6 is a view showing the results of measuring the ABTS radical scavenging ability according to the phenolic concentration in the water and ethanol extract of the greenball apple peel and the adverb apple peel according to an embodiment of the present invention.
7 is a view showing the results of measuring the PF by phenolic concentration in the water and ethanol extract of the greenball apple peel and adverb apple peel in accordance with an embodiment of the present invention.
8 is a view showing the TBARs inhibitory effect according to phenolic concentrations in water and ethanol extracts of greenball apple peel and adverb apple peel according to an embodiment of the present invention.
9 is a view showing the hyaluronidase inhibitory effect of phenolic concentrations in water and ethanol extracts of greenball apple peel and adverb apple peel according to one embodiment of the present invention.
Figure 10a is a view showing the elastase inhibitory effect of phenolic concentrations in water and ethanol extracts of greenball apple peel and adverb apple peel according to an embodiment of the present invention.
Figure 10b is a view showing the collagenase inhibitory effect according to phenolic concentration in water and ethanol extracts of greenball apple peel and adverb apple peel according to an embodiment of the present invention.

As the invention allows for various changes and numerous embodiments, particular embodiments will be illustrated in the drawings and described in detail in the written description. However, this is not intended to limit the present invention to specific embodiments, it should be understood to include all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In the following description of the present invention, if it is determined that the detailed description of the related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted.

Hereinafter, the present invention will be described in detail.

The present invention for antioxidant comprising a greenball apple peel extract as an active ingredient; Anti-inflammatory; Or it provides a pharmaceutical composition for wrinkle improvement.

In the present invention, an "extract" is an extract obtained by the extraction process of the greenball apple peel, a diluent or concentrate of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, a mixture thereof, and the like. , Extracts themselves and extracts of all formulations that can be formed using the extracts.

In the greenball apple peel extract of the present invention, the method of extracting the greenball apple peel is not particularly limited and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method and the like, these may be performed alone or in combination of two or more methods.

The kind of the extraction solvent used to extract the greenball apple peel in the present invention is not particularly limited, and according to a conventional method known in the art for extracting the extract from natural products, that is, the conventional temperature, pressure Extraction can be carried out using conventional solvents under conditions. For example, the greenball apple peel extract in the present invention is water, lower alcohol having 1 to 4 carbon atoms, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and a mixed solvent thereof Can be extracted using one or more solvents selected from the group consisting of, according to one embodiment of the present invention can be extracted using water or ethanol, but is not limited thereto.

The prepared extract may then be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. For example, the filtration may use a filter paper or a vacuum filter, the concentration may be a vacuum concentrator, the drying may be a freeze-drying method, but is not limited thereto.

In addition, the present invention provides an antioxidant comprising administering the pharmaceutical composition to a subject; Anti-inflammatory; Or it provides a wrinkle improvement method.

As used herein, the term "administration" means providing a subject with any composition of the present invention in any suitable manner.

As used herein, the term "individual" refers to a subject in need of antioxidant, anti-inflammatory, or anti-wrinkle, and more specifically, human or non-human primates, mice, dogs, cats, horses And mammals such as cows.

In addition, the present invention provides antioxidants of the pharmaceutical composition; Anti-inflammatory; Or anti-wrinkle applications.

In the present invention, the "pharmaceutical composition" means that it is prepared for the purpose of antioxidant, anti-inflammatory, or wrinkle improvement, each can be used in formulated in various forms according to conventional methods. For example, they may be formulated in oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and the like, creams, gels, patches, sprays, ointments, warnings, lotions, liniments, pasta or It may be formulated in the form of external preparations such as cataplasma, suppositories, and sterile injectable solutions.

The pharmaceutical compositions according to the invention may further comprise suitable carriers, excipients and diluents conventionally used in the preparation of pharmaceutical compositions. At this time, carriers, excipients and diluents that may be included in the composition include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium Silicates, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. Or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The pharmaceutical compositions of the invention may be administered orally or parenterally (eg, applied intravenously, subcutaneously, intraperitoneally or topically) according to the desired method, and the dosage may be determined by the condition and weight of the patient, Depending on the degree, drug form, route of administration, and time, it may be appropriately selected by those skilled in the art.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. As used herein, the term “pharmaceutically effective amount” means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level refers to the type of disease, severity, activity of the drug, It may be determined according to the sensitivity to the drug, the time of administration, the route of administration and the rate of release, the duration of treatment, factors including the drug used concurrently and other factors well known in the medical field. The pharmaceutical compositions according to the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be single or multiple doses. Taking all of the above factors into consideration, it is important to administer an amount that can achieve the maximum effect with a minimum amount without side effects, which can be readily determined by one skilled in the art. Administration may be administered once a day or may be divided several times.

In addition, the present invention for the antioxidant containing a green ball apple peel extract as an active ingredient; Anti-inflammatory; Or it provides a functional food composition for improving wrinkles.

As used herein, the term “improvement” means any action that at least reduces the parameters associated with the condition being treated, for example the extent of symptoms.

In the present invention, the "health food composition" is characterized in that it is formulated as one selected from the group consisting of tablets, pills, powders, granules, powders, capsules and liquid formulations, including one or more of carriers, diluents, excipients and additives. It is done.

Foods that can be added to the greenball apple peel extract of the present invention include various foods, powders, granules, tablets, capsules, syrups, beverages, gums, teas, vitamin complexes, and health functional foods. As additives that may be further included in the present invention, natural carbohydrates, flavoring agents, nutrients, vitamins, minerals (electrolytes), flavoring agents (synthetic flavoring agents, natural flavoring agents, etc.), colorants, fillers, pactic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, antioxidants, glycerin, alcohols, carbonation agents, and one or more components selected from the group consisting of pulp can be used. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used.

In addition to the above, the composition according to the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents, fact acids and salts thereof, alginic acid and salts thereof, organic acids, protection Sex colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages and the like.

In addition, the compositions according to the invention may contain flesh for the production of natural fruit juices and vegetable beverages. These components can be used independently or in combination.

Specific examples of the carrier, excipient, diluent and additives include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium phosphate, calcium silicate, Group consisting of microcrystalline cellulose, polyvinylchirrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methyl hydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate, and mineral oil It is preferred that at least one selected from be used.

In addition, the present invention for the antioxidant containing a green ball apple peel extract as an active ingredient; Anti-inflammatory; Or to provide a cosmetic composition for improving wrinkles.

Cosmetic composition comprising the greenball apple peel extract of the present invention can be used in a variety of cosmetics and cleansing agents for the skin's antioxidant, anti-inflammatory, anti-wrinkle effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

The cosmetic of the present invention comprises a composition selected from the group consisting of water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extracts.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins usable in the present invention. Included. The water-soluble vitamin can be obtained by conventional methods such as microbial transformation, purification from culture of microorganisms, enzymatic or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. , Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or a fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Colorginine, arginate, sodium arginate, purified from these seaweed extracts, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octylate decyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopenta sorbitol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristic acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethyl hexateate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol phthalic acid, neopentyl glycol dicapricate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tritridecylate, glyceryl triisopalmitine, glyceryl triisostearate, octyldodecyl neopentane Isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerin isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-stealo Ester type | system | groups, such as monohydroxystearic acid isostearyl, etc. are mentioned.

Hydrocarbon-based oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and petrolatum, etc. are mentioned as a hydrocarbon oil-based fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

As a humectant, a water-soluble low molecular humectant, a fat-soluble molecular moisturizer, a water-soluble polymer, a fat-soluble polymer, etc. are mentioned.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long chain acyl glutamic acid cholesteryl ester, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Examples of nonionic surfactants are self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, and POE. Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate , Alkyloyl alkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfo acetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. are mentioned. .

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments, such as a silk powder, a cellulose, CI pigment yellow, and CI pigment orange, and the composite pigment of these inorganic pigments and organic pigments, etc. are mentioned.

As organic powder, Metal soap, such as calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolysine, N-alpha-paratoylolnitine, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Cananoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hydroxy Ci-5-methylphenyl) benzotriazole and the like.

Examples of fungicides are hinokithiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinphylthione, benzalkonium chloride and photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

As alcohol, higher alcohols, such as cetyl alcohol, are mentioned.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, any of the above components can also be mix | blended within the range which does not impair the objective and effect of this invention.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, plant fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In an experimental example of the present invention, as a result of comparing the physiological activities of the greenball apple peel and whole apple extract, it was confirmed that the greenberry apple peel extract had the highest physiological activity (see Experimental Example 1).

In another experimental example of the present invention, as a result of comparing the solid and phenolic physiological activities of the greenball apple peel and whole apple extract, it was confirmed that the phenolic compounds of the greenball apple peel were directly involved in the physiological activity (see Experimental Example 2). ).

In another experimental example of the present invention was compared the extraction yield of phenolic compound by solvent type, concentration of greenball apple.

Extraction is a method of separating the physiologically active substances and functional substances contained in the sample according to the characteristics of each solvent, the setting of the solvent used in the extraction has an important effect on the pre-bioactivity experiment. Hot water extraction using pure distilled water has no problem of harmfulness to solvents, high yield, and is widely used for extracting water-soluble substances.In the case of ethanol extraction, which is a polar solvent, it is easy to purify after extraction, and tannin, saponin, and organic acid of plants. It is mainly used for the extraction of various useful components.

The extracts of greenball apples from different types of solvents were measured for the phenolic content, and the highest yields were obtained in the order of methanol, ethanol, and water, and the highest yields were obtained for 70% ethanol extracts. , Greenball whole apple was confirmed to show the highest extraction yield in 60% ethanol extract (see Experimental Example 3).

In another experimental example of the present invention, the antioxidant effect of the greenball apple peel extract was confirmed.

Antioxidants neutralize free radicals before they attack DNA or oxidize lipids, causing harmful reactions in the cells of the body. Therefore, antioxidants are one of the functional or physiologically active substances that human beings are currently paying attention to, and are known as substances capable of preventing the deterioration of food, preventing aging in the human body, and preventing adult diseases. Antioxidants are highly reactive and react with harmful substances in the body to protect the cells by blocking the chain reaction caused by free radicals. The body contains antioxidant enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), and low-molecular antioxidant vitamin C, which acts as an antioxidant or free radical scavenger. Several minerals, including vitamin E, β-carotin, carotenoids, flavonoids, and selenium, are known to protect the body.

In order to confirm the antioxidant effect of Greenball apple peel extract, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging ability, Antioxidant protection factor (PF) and Thiobarbituric acid reactive substances (TBARs) were measured and confirmed, Green Apple apple bark extract showed an excellent antioxidant effect compared to the adverb apple bark extract (see Experimental Example 4).

In another experimental example of the present invention, the anti-inflammatory effect of the greenball apple peel extract was confirmed.

The hyaluronic acid (HA) of the polymer inhibits the phagocytic ability of macrophage, which is an important factor of inflammation formation, whereas the HA breakdown or small molecule HA increases inflammation, fibrosis and collagen deposition during wound healing. In addition, hyaluronidase (HAase) is generally present in lysosomes in an inactive form to maintain homeostasis. However, hyaluronidase (HAase) is known as an inflammation-inducing substance because it is activated when inflammatory diseases such as physical injury or rheumatism are involved and is involved in vascular permeability and inflammatory response. Allergic reactions and cancer cell metastasis have been reported.

Thus, the hyaluronidase inhibitory effect was measured to evaluate the anti-inflammatory effect of the greenball apple peel extract, and the greenball apple peel extract showed a superior anti-inflammatory effect because the hyaluronidase inhibition effect was higher than that of the adverb peel extract ( See Experimental Example 5).

In another experimental example of the present invention confirmed the wrinkle improvement effect of the greenball apple peel extract.

The elastin, a structural protein, decreases as the skin ages, and the biosynthesis of type-1 collagenase increases according to the degree of skin aging, leading to degradation of matrix proteins such as collagen fibers and elastic fibers in the dermis. Knocks down and causes the formation of skin wrinkles. Elastase, which is present in human neutrophil granulocytes, is an enzyme that degrades skin elastic fibers (elastin). Inhibition of elastase means improvement of skin wrinkles.

In addition, collagen has the function of inducing mechanical strength of skin, resistance of tissue, tissue power, cell division and differentiation.Increasing stress due to internal factors such as decreased cell activity due to natural aging and harmful environment, UV irradiation Decomposed by external factors such as photoaging. This decrease in collagen is reported to reduce the elasticity of the skin and cause wrinkles and sagging.

As a result of measuring the elastase and collagenase inhibitory effects to evaluate the antiwrinkle effect of greenball apple peel extract, the greenball apple peel extract showed better antiwrinkle effect than elastase and collagenase inhibitory effect compared to the adverb peel extract. It was confirmed (see Experimental Example 6).

Hereinafter, preferred embodiments and experimental examples of the present invention will be described in detail with reference to the accompanying drawings. However, these Examples and Experimental Examples are only for illustrating the present invention, and the scope of the present invention will not be construed as being limited by these Examples and Experimental Examples.

Example  1. Material Preparation

1-1. Green Ball  Apple Sample Preparation

The test materials used were Greenball apples, which are newly grown varieties that were bred by breeding Golden Delicious (Fubon) and Fuji (Fubon) as breeding combinations at the Apple Institute in Gyeongbuk County. The samples were harvested from green ball apple trees, and the foreign matters were removed and the skins and whole apples of apples were tested. Peel and whole apple samples were lyophilized (freeze dryer, FD8518, Ilshinbiobase, Yangju, Korea) to remove moisture and then crushed into 40 mesh and stored at 4 ℃ low temperature and used as samples.

1-2. Green Ball  From an apple phenolic  Extract manufacturer

Preparation of extracts for the analysis of functional foods and functional cosmetics activity, in the case of hot water extracts, 1 g of the sample powder of greenball apples prepared in Example 1-1 was immersed in 200 mL of distilled water and heated until the extract became 100 mL and then cooled. The extract was stirred for 24 hours, and in the case of ethanol extract, 100 mL of 10-100% ethanol was added to 1 g of the greenball apple powder, followed by stirring extraction for 24 hours in a shaking incubator at 4 ° C. Each extract contains Whatman No. 1 filter paper (Whatman inc., Piscataway, New jersey, USA), and then, if necessary, concentrate in a rotary vacuum evaporator (Eyela NE, Tokyo, Japan) and store at low temperature in a 4 ℃ refrigerator. 25, 50, 75, 100 µg / mL or 50, 100, 150, 200 µg / mL phenolics concentrations were used for the experiment, respectively.

Example  2. Experimental method

2-1. Total phenolic compound content quantification

For total phenolic quantification, 1 mL of 95% ethanol and 5 mL of distilled water were added to 1 mL of extract according to the method of Folin and Denis. After mixing 0.5 mL of 1 N Folin-ciocalteu reagent for 5 minutes, 1 mL of Na ₂ CO ₃ was added. The absorbance was measured within 1 hour at 725 nm, and the amount was converted from the standard curve using gallic acid.

2-2. 1,1- diphenyl -2- picrylhydrazyl  ( DPPH ) Radical Inhibition Effect Measurement

DPPH radical inhibition effect according to the method of Blois, immersed 3 mL of 60 μM DPPH in 0.5 mL of the sample and allowed to stand for 15 minutes and the absorbance was measured at 517 nm.

2-3. 2,2'- Azinobis -(3- ethylbenzothiazoline -6- sulfonic  acid) ( ABTS ) Radical Inhibition Effect Measurement

ABTS radical inhibition effect according to the method of Fellegrini et al., Mixed with 7 mM ABTS and 140 mM K ₂ S ₂ O ₈ in 5 mL: 88 μL and left in a dark place for 12-16 hours, and then ethanol 1:88 The mixture was mixed at a ratio of 734 nm, and the absorbance value of the control was adjusted to 0.7 ± 0.02. The ABTS solution was mixed with 50 μL of sample solution and 1 mL of ABTS solution for 30 seconds and then incubated for 2.5 minutes to measure absorbance at 734 nm.

2-4. Antioxidant protection factor ( PF ) Measure

PF measurement was carried out according to Andarwulan and Shetty's method. 1 mL of a solution of 10 mg β-carotene in 50 mL of chloroform was placed in an evaporator water, and chloroform was distilled in a 40 ° C water bath. 20 μL linoleic acid, 184 μL Tween 40 and 50 mL H ₂ O ₂ were added to make an emulsion. 100 μL of the sample was mixed with 5 mL of emulsion, mixed well with a vortex, reacted at 50 ° C. for 30 minutes, cooled, and the absorbance was measured at 470 nm. .

2-5. Thiobarbituric  acid reactive substances ( TBARs ) Inhibition effect measurement

TBARs inhibition effect was made by 1% linoleic acid and 1% Tween 40 according to Buege and Aust method, 0.8 mL of emulsion and 0.2 mL of sample were mixed and reacted for 10 hours in a 50 ℃ water bath. 2 mL of TBA regent was added to 1 mL of the reaction solution, which was then stirred for 15 minutes, cooled for 10 minutes, centrifuged at 1,000 rpm for 15 minutes, and allowed to stand at room temperature for 10 minutes. The supernatant was measured for absorbance at 532 nm.

2-6. Hyaluronidase  Inhibition effect measurement

Hyaluronidase (HAase) inhibitory effect was measured by absorbance at 600 nm by modifying N-acetyl-glucosamine formed from sodium-hyaluronic acid (HA) to glucoxazoline derivative followed by color development with ρ-dimethylaminobenzaldehyde (DMAB) according to Dorfman and OTT method. It was.

2-7. Elastase  Inhibition effect measurement

Elastase inhibitory effect was 1.0 U / mL in a mixture of 0.1 mL of 0.8 mM N-succinyl- (Ala) 3-ρ-nitroanilide solution in 1 mL of 0.2 M Tris-HCl buffer (pH 8.0) according to Kraunsoe et al. Add 0.1 mL of porcine pancreatic elastase (PPE) (Sigma-Aldrich Co.) enzyme solution and 0.1 mL of each sample of 50 ~ 200 μg / mL phenolic compounds, and add 0.1 mL of distilled water instead of the sample to the control. After the ρ-nitroaniline production was measured at absorbance 410 nm.

2-8. Collagenase  Inhibition effect measurement

Collagenase inhibitory effect was obtained by adding 4 mM CaCl ₂ to 0.1 M Tris-HCl buffer (pH 7.5) according to Wunsch and Heidrich's method (4-mgazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (0.3 mg / mL). ) 0.15 mL of 0.2 mg / mL collagenase (Sigma-Aldrich Co.) was added to a mixed solution of 0.25 mL of dissolved substrate solution and 0.1 mL of each sample solution having a concentration of 50 to 200 μg / mL phenolic compounds. 0.5 mL of 6% citric acid was added to stop the reaction. 2 mL of ethyl acetate was added thereto, and the absorbance was measured at 320 nm.

2-9. Statistical analysis

Statistical analysis was performed by Duncan's multiple range test, and the different superscript characters in the graph indicate significant differences at P <0.05.

Experimental Example  One. Green Ball  Comparison of Biological Activities between Apple Peel and Whole Apple Extract

As a result of measuring the phenolic content by the method of Example 2-1 by extracting the peel of greenball apples and whole apples with water and 60% ethanol by the method of Example 1-2, as shown in Figure 1 It showed the highest phenolic content. Therefore, it was speculated that the physiological activity of the bark extract showed the highest level.

  In addition, in order to evaluate the physiological effects of the peel of greenball apples and the whole apple extract, the collagenase inhibitory effect, which is one of the anti-wrinkle effects, was measured by the method of Example 2-8, and as shown in FIG. Efficacy was high in, and low levels of collagenase inhibition were found in whole apples containing flesh.

In the graph of FIG. 2, PW is peel water extracts of greenball apples, PE is peeled ethanol extracts of greenball apples, and WhW is whole fruit water exracts of greenball whole apples. WhE refers to whole fruit ethanol extracts of greenball whole apples.

According to the results, when comparing the green apples with the peel of apples, the phenolic content was higher in the peel, and the peel improved activity than the whole apple containing the flesh was confirmed that the wrinkles showed higher wrinkle improvement activity. Therefore, the experiment was carried out in the experiment of the skin of the apple of greenball was high efficacy.

Experimental Example  2. Green Ball  Comparison of Physiological Activities of Solid and Phenolic Extracts from Apple Peel and Whole Apple Extract

As a result of measuring the DPPH radical scavenging ability by the method of Example 2-2 for comparing the physiological activity effect of the solid content of the apple and the phenolic component, 100 ㎍ / mL concentration of the greenball apple peel water and ethanol extract as shown in FIG. In the solid content of, it showed 58.95% and 69.28% electron donating ability, respectively, but it showed the electron donating ability of 92.47% and 80.62% at 100 ㎍ / mL phenolic concentration, respectively, and showed activity by phenolic compound. In the 100 ㎍ / mL solid content of the ethanol extract showed 25.84% and 29.62% electron donating ability, respectively, as shown in Figure 3b, but 90.78% and 84.01% electron donating ability at 100 ㎍ / mL phenolic concentration, respectively It was found to be active by the compound.

In addition, as a result of measuring ABTS radical scavenging ability by the method of Example 2-3 to compare the physiological activity effect of the solid content and phenolic component, as shown in Figure 3c of apple peel water, ethanol extract 100 ㎍ / mL concentration The solids showed electron donating abilities of 9.93% and 9.53%, respectively, and the electron donating abilities of 89.15% and 83.08% at 100 ㎍ / mL phenolic concentration, respectively, and the solid content of 100 ㎍ / mL of water and ethanol extracts of whole apples. In Fig. 3d, the electron donating ability of 5.61% and 4.02% was shown, respectively, but the electron donating ability of 90.34% and 91.44% at 100 ㎍ / mL phenolic concentration, respectively, was confirmed to be active by the phenolic compound. The physiological activity of apples was again confirmed to be governed by phenolic compounds in solids.

Therefore, as a result of comparing the physiological activity of the solid content and phenolic components, it was confirmed that the phenolic compounds of the apple peel is directly involved in the physiological activity, and the experiment was conducted by extracting the phenolic compound of the apple peel.

Experimental Example  3. Green Ball  Comparison of Extraction Yields of Phenol Compounds by Apple Solvent Type and Concentration

As a result of measuring the phenolic content by extracting the peel of greenball apple by the type of solvent, as shown in Figure 4a, methanol, ethanol, water, acetone, butanol, ethyl acetate in the order 7.46, 6.38, 5.30, 3.31, 3.24, 2.36 The phenolic content of mg / g was shown, and the extract of green berry apple peel by ethanol concentration showed the highest extraction yield of phenolic content as 8.31 mg / g in 70% ethanol extract as shown in FIG. 4b.

In addition, the ethanol concentration of the greenball whole apple extract showed the highest extraction yield of phenolic content as 3.49 mg / g in 60% ethanol extract as shown in Figure 4c.

Therefore, the extract of greenball apples and whole apples using water, 70% ethanol, and 60% ethanol as solvents were used for the extraction.

The difference in the content of phenolic compounds between the peels of greenball apples and whole apple extracts was about 2.3 times higher than that of whole apple extracts.

According to the results, the extract extracted with water, 70% and 60% ethanol was tested for antioxidant, anti-inflammatory, anti-wrinkle activity by adjusting the phenolic content to 25 ~ 200 ㎍ / mL for reproducibility of the experiment.

Experimental Example  4. Green Ball  Antioxidant Effect of Apple Peel Extract

4-1. 1,1- diphenyl -2- picrylhydrazyl  ( DPPH ) Inhibition of radical inhibition effect

DPPH radical scavenging activity was measured by the method of Example 2-2 to evaluate the antioxidant effect of greenball apple peel extract, as shown in FIG. 5, water extract (GPW) and ethanol extract (GPE) of greenball apple peel ) Showed radical scavenging ability of 82.94 ~ 88.26 and 89.56 ~ 100.00% at 25 ~ 100 ㎍ / mL phenolic concentration, respectively. In addition, the water extract (BPW) and ethanol extract (BPE) of the bark of the adverb varieties used as a control showed 85.16 to 87.55 and 87.80 to 94.40% radical scavenging activity, and the green ball apple peel extract was better than the adverb varieties. The donor ability was found to be excellent DPDP radical inhibitory effect.

4-2. 2,2'- Azinobis -(3- ethylbenzothiazoline -6- sulfonic  acid) ( ABTS ) Inhibition of radical inhibition effect

As a result of measuring the ABTS radical scavenging ability by the method of Example 2-3 using the green ball apple peel extract, as shown in Figure 6 of the water extract (GPW), and ethanol extract (GPE) of the green ball apple peel At ˜100 ㎍ / mL phenolic concentration, 44.36 ~ 99.27 and 41.99 ~ 99.09% radical scavenging activity were observed. In addition, the water extract (BPW) and ethanol extract (BPE) of the adverb varietal bark used as a control showed radical scavenging ability of 40.15 ~ 99.46, 38.70 ~ 98.22%, and showed electron donating ability similar to that of greenball apple peel extract. Likewise, greenball apple peel extracts showed very high activity in DPPH and ABTS radical scavenging ability.

4-3. Antioxidant protection factor ( PF ) Confirm

Β-carotine, which is composed of 11 double bonds and has high unsaturated properties, reacts very easily with peroxyl radicals, and it shows a fat-soluble antioxidant activity by using selective chain cleavage to one of the unsaturated carbons and acting as an antioxidant. PF was measured by the method of Example 2-4.

As a result, as shown in Figure 7, the greenball apple peel water extract (GPW), and ethanol extract (GPE) of 1.23 ~ 1.43, 1.19 ~ 1.21 PF at 25 ~ 100 ㎍ / mL phenolic concentrations, respectively, the control group Water extract (BPW) and ethanol extract (BPE) of the adverb varietal bark were used as 1.22 ~ 1.29 and 1.26 ~ 1.37 PF at 25 ~ 100 ㎍ / mL phenolic concentration, respectively. It was confirmed that this shows a better fat-soluble antioxidant capacity.

4-4. Thiobarbituric  acid reactive substances ( TBARs ) Confirmation of Inhibitory Effect

As a result of measuring the inhibitory activity of TBARs showing another fat-soluble antioxidant activity using the greenball apple peel extract by the method of Example 2-5, as shown in FIG. 8, the water extract (GPW) of the greenball apple peel, And ethanol extract (GPE) showed inhibitory activity of TBARs of 24.88 ~ 60.29 and 29.66 ~ 75.92%, respectively. In addition, the water extract (BPW) and ethanol extract (BPE) of the adverb varieties bark used as a control showed the inhibitory activity of TBARs of 32.60 ~ 55.40, 20.57 ~ 72.48%, respectively. From the above results, it was confirmed that the greenball apple peel extract has a better fat-soluble antioxidant capacity than the apples of the adverb varieties.

Therefore, the greenball apple peel extract has a high antioxidant activity compared to the adverb varieties, it was determined that it can be used as a functional material for anti-aging.

Experimental Example  5. Green Ball  Anti-inflammatory Effects of Apple Peel Extracts

In order to confirm the anti-inflammatory effect of Greenball apple peel extract, the inhibitory effect of hyaluronidase (HAase), an enzyme related to inflammation, was measured by the method of Example 2-6.

As a result, as shown in Figure 9 green water apple peel (GPW) showed an inhibitory effect of 0.00 ~ 6.45% at a phenolic concentration of 50 ~ 200 ㎍ / mL, ethanol extract (GPE) of greenball apple peel ) Showed an inhibitory effect of 13.16-20.78% at a phenolic concentration of 50-200 ㎍ / mL. In addition, the water extract (BPW) of the adverb varietal bark used as a control showed an inhibitory effect of 0.03-1.79% at a phenolic concentration of 50-200 ㎍ / mL, and 50-200 ㎍ in the ethanol extract (BPE) of the adverb varietal bark. phenolic concentration of 1.29 ~ 12.72%, green ball apple peel extract showed better anti-inflammatory effect than adverb shell extract, especially ethanol extract showed higher anti-inflammatory effect than adverb. Indicated.

 Therefore, from the above results, it was determined that the greenball apple peel extract could be applied to a functional product utilizing anti-inflammatory and atopic inhibitory effects.

Experimental Example  6. Green Ball  Confirmation of wrinkle improvement of apple peel extract

6-1. Elastase  Confirmation of inhibitory effect

As a result of measuring the inhibitory effect of elastase, an enzyme that decomposes elastin to maintain skin elasticity of the human body and produces skin wrinkles, the water extract of greenball apple peel as shown in FIG. GPW) showed 3.90 ~ 4.55% inhibitory effect at phenolic concentration of 50 ~ 200 ㎍ / mL, and 29.39 ~ 63.38% at phenolic concentration of 50 ~ 200 ㎍ / mL in ethanol extract (GPE) of greenball apple peel. It showed a relatively high inhibitory effect. In addition, the bark water extract (BPW) of the adverb varieties used as a control showed an inhibitory effect of 0.00 ~ 5.35% at a phenolic concentration of 50-200 ㎍ / mL, and 50-200 ㎍ / mL in the ethanol extract (BPE). In the phenolic concentration of 8.02 ~ 32.10% showed an inhibitory effect, it was confirmed that the elastase inhibitory effect of Greenball apple peel extract is better.

6-2. Collagenase  Confirmation of inhibitory effect

As a result of measuring the inhibitory effect of collagenase, an enzyme that degrades collagen, by the method of Example 2-8, as shown in FIG. 10B, the water extract (GPW) of greenball apple peel shows a phenolic concentration of 50-200 ㎍ / mL. Showed an inhibitory effect of 8.44 ~ 40.06%, and ethanol extract (GPE) of greenball apple peel showed 44.66 ~ 80.07% of inhibitory effect at 50 ~ 200 ㎍ / mL phenolic concentration. In addition, the water extract (BPW) of the adverb varieties used as a control showed an inhibitory effect of 25.89-59.73% at a phenolic concentration of 50-200 ㎍ / mL, and 50-200 ㎍ / mL in the adverb shell ethanol extract (BPE). In the phenolic concentration of 47.79 ~ 79.05% inhibitory effect, it was confirmed that the ethanol extract of greenball apple peel has a better collagenase inhibitory effect than the adverb varieties in the 100 ~ 150 μg / mL phenolic concentration range.

Therefore, Greenball apple peel extract from the above results to inhibit the elastase and collagenase enzymes affecting wrinkles by decomposing elastin and collagen, which are important proteins for maintaining skin elasticity, have a positive effect on skin wrinkle improvement effect Confirmed.

The specific parts of the present invention have been described in detail above, and it is apparent to those skilled in the art that such specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Therefore, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (15)

Antioxidant for containing green ball apple peel extract as an active ingredient; Anti-inflammatory; Or anti-wrinkle pharmaceutical composition.
The method of claim 1,
The composition contains 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical inhibition, 2,2'-azinobis- (3-ethylbenzothiazoline-6- Sulfonic acid) (2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid; ABTS) radical inhibition, Thiobarbituric acid reactive substances (TBARs) inhibition or antioxidant protection factor PF), pharmaceutical composition.
The method of claim 1,
The composition is characterized by inhibiting hyaluronidase (hyaluronidase), pharmaceutical composition.
The method of claim 1,
The composition is characterized in that it inhibits elastase or collagenase (collagenase), pharmaceutical composition.
The method of claim 1,
The greenball apple peel extract is selected from the group consisting of water, C ₁ to C ₄ lower alcohols, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and mixed solvents thereof. Pharmaceutical composition, characterized in that extracted with a selected solvent.
Antioxidant for containing green ball apple peel extract as an active ingredient; Anti-inflammatory; Or functional food composition for wrinkle improvement.
The method of claim 6,
The composition contains 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical inhibition, 2,2'-azinobis- (3-ethylbenzothiazoline-6- Sulfonic acid) (2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid; ABTS) radical inhibition, Thiobarbituric acid reactive substances (TBARs) inhibition or antioxidant protection factor PF), nutraceutical composition, characterized in that to increase.
The method of claim 6,
The composition is characterized by inhibiting hyaluronidase (hyaluronidase), dietary supplement composition.
The method of claim 6,
The composition is characterized in that it inhibits elastase or collagenase (collagenase), dietary supplement composition.
The method of claim 6,
The greenball apple peel extract is selected from the group consisting of water, C ₁ to C ₄ lower alcohols, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and mixed solvents thereof. Health functional food composition, characterized in that extracted with a selected solvent.
Antioxidant for containing green ball apple peel extract as an active ingredient; Anti-inflammatory; Or cosmetic composition for wrinkle improvement.
The method of claim 11,
The composition contains 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical inhibition, 2,2'-azinobis- (3-ethylbenzothiazoline-6- Sulfonic acid) (2,2'-Azinobis- (3-ethylbenzothiazoline-6-sulfonic acid; ABTS) radical inhibition, Thiobarbituric acid reactive substances (TBARs) inhibition or antioxidant protection factor PF) to increase the cosmetic composition.
The method of claim 11,
The composition is characterized in that to inhibit hyaluronidase (hyaluronidase), cosmetic composition.
The method of claim 11,
The composition is characterized in that it inhibits elastase or collagenase, the cosmetic composition.
The method of claim 11,
The greenball apple peel extract is selected from the group consisting of water, C ₁ to C ₄ lower alcohols, n-hexane, ethyl acetate, acetone, butyl acetate, 1,3-butylene glycol, methylene chloride, and mixed solvents thereof. Cosmetic composition, characterized in that extracted with a selected solvent.

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