WO2023282355A1 - 魚類飼育用組成物、および魚病に対する予防または治療用組成物 - Google Patents

魚類飼育用組成物、および魚病に対する予防または治療用組成物 Download PDF

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WO2023282355A1
WO2023282355A1 PCT/JP2022/027146 JP2022027146W WO2023282355A1 WO 2023282355 A1 WO2023282355 A1 WO 2023282355A1 JP 2022027146 W JP2022027146 W JP 2022027146W WO 2023282355 A1 WO2023282355 A1 WO 2023282355A1
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gene
plantaricin
nisin
fish
plantalysin
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English (en)
French (fr)
Japanese (ja)
Inventor
幹雄 青木
和樹 味方
敏裕 甲斐
弘 桑原
育生 廣野
秀裕 近藤
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Tokyo University of Marine Science and Technology NUC
Sumitomo Chemical Co Ltd
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Tokyo University of Marine Science and Technology NUC
Sumitomo Chemical Co Ltd
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Application filed by Tokyo University of Marine Science and Technology NUC, Sumitomo Chemical Co Ltd filed Critical Tokyo University of Marine Science and Technology NUC
Priority to CN202280048254.3A priority Critical patent/CN117915781A/zh
Priority to JP2023533204A priority patent/JPWO2023282355A1/ja
Priority to EP22837769.3A priority patent/EP4413867A4/en
Priority to KR1020247003675A priority patent/KR20240034202A/ko
Priority to AU2022307811A priority patent/AU2022307811A1/en
Priority to US18/577,017 priority patent/US20250099514A1/en
Priority to CA3226285A priority patent/CA3226285A1/en
Publication of WO2023282355A1 publication Critical patent/WO2023282355A1/ja
Anticipated expiration legal-status Critical
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/195—Antibiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/335—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Definitions

  • the present invention relates to fish breeding compositions and preventive or therapeutic compositions for fish diseases.
  • the present invention also relates to a method for rearing fish organisms using the above composition.
  • FIG. 10 shows (A) colony shape and (B) Gram staining results of NITE ABP-03481 in Experiment 6.
  • FIG. 10 is a diagram explaining an antibacterial activity evaluation test against Lactococcus garvieae in Experiment 7.
  • FIG. 10 is a diagram explaining an antibacterial activity evaluation test against Lactococcus garvieae in Experiment 7.
  • Substitutions considered conservative substitutions include Ala to Ser or Thr, Arg to Gln, His or Lys, Asn to Glu, Gln, Lys, His or Asp, Asp to Asn, Glu. or Gln substitution, Cys to Ser or Ala substitution, Gln to Asn, Glu, Lys, His, Asp or Arg substitution, Glu to Gly, Asn, Gln, Lys or Asp substitution, Gly to Pro His to Asn, Lys, Gln, Arg or Tyr, Ile to Leu, Met, Val or Phe, Leu to Ile, Met, Val or Phe, Lys to Asn, Glu , Gln, His or Arg, Met to Ile, Leu, Val or Phe, Phe to Trp, Tyr, Met, Ile or Leu, Ser to Thr or Ala, Thr to Ser or Ala substitution, Trp to Phe or Tyr substitution, Tyr to His, Phe or Trp substitution, and Val to Met, Ile or Leu
  • plantalysin may be a peptide having an amino acid sequence in which part or all of Trp-Gly-Trp is added to the C-terminus of the amino acid sequence set forth in SEQ ID NO:1.
  • Plantalysin is a peptide having an amino acid sequence in which part or all of Lys-Ser-Ile-Arg or part or all of Asn-Ile-Arg is added to the C-terminus of the amino acid sequence shown in SEQ ID NO: 2.
  • the plantaricin gene includes plantaricin A gene (e.g., gene having the base sequence of SEQ ID NO: 20, 30, or 40), plantaricin E gene (e.g., base sequence of SEQ ID NO: 8, 22, 32, 42, or 50).
  • ATCC-11454 and NITE-ABP-03481 are examples of bacteria that have the nisin gene.
  • ATCC-11454 is a kind of lactic acid bacteria Lactococcus lactis subsp. lactis.
  • NITE-ABP-03481 is the receipt number NITE ABP-03481 (date of receipt: June 9, 2021), National Institute of Technology and Evaluation Patent Microorganisms Depositary Center (NPMD, Address: Kisarazu, Chiba Prefecture 292-0818) It is a bacterium that has been internationally deposited under the Budapest Treaty at Room 122, 2-5-8 Kamatari, Kazusa City.
  • NITE-BP-03198 is present in fermented foods
  • NITE-BP-03199, NITE-BP-03200, NITE-BP-03201, NITE-BP-03202 and NITE-ABP-03481 are present in the natural environment, It is considered to have little impact on the environment when used for breeding fish organisms, and to be highly safe to humans.
  • the bacterium may be an isolated bacterium.
  • the bacterial cell or bacterial cell culture extract is prepared so as not to lose the antibacterial activity against Lactococcus garvie that the bacterial cell or bacterial cell culture of the lactic acid bacterium has.
  • Extracts can be obtained, for example, by subjecting bacterial cells or cell cultures to ultrasonic disruption, bead grinding, freeze-thawing, chemical lysis, or the like.
  • the extract may be obtained by salting-out, ultrafiltration, ion-exchange chromatography, liquid-phase extraction using an organic solvent, or the like, on the cells or cell culture. These treatments can be performed in combination as appropriate.
  • the extract may contain bacterial cell fragments, nucleic acids, peptides, proteins, sugars and enzymes.
  • bacterial cells, bacterial cell cultures, or extracts thereof are also referred to as "microbial cell preparations.”
  • the horse mackerel family includes the genus Seriola (Japanese amberjack, greater amberjack, amberjack, greater amberjack, etc.), the genus striped jack (striped jack, etc.), and the genus jack mackerel (striped jack, etc.).
  • the Mackerel family includes Mackerel genus (comb, mackerel, Atlantic mackerel, etc.) and Tuna genus (bluefin tuna, Atlantic tuna, yellowfin tuna, etc.).
  • the family Perciaceae includes the genus Percius (such as Perch).
  • the orally administered composition according to the present invention can reduce the burden of administration, as compared with the conventional method for controlling fish diseases in which vaccines are injected one by one.
  • the orally administered composition according to the present invention can be used for rearing a wide range of species without being limited to fish species.
  • the fish feed may contain any component as long as it is a component normally used for breeding and aquaculture of fish organisms, and may be manufactured by any manufacturing method.
  • the composition of the fish feed can be appropriately selected depending on the type and growth stage of the fish.
  • Fish feeds may include protein sources such as squid meal, krill meal, fish meal, soybean meal, corn gluten meal, and binders such as gluten and starch.
  • Fish feeds may contain known carriers or additives that are acceptable for feeds, and include erythromycin preparations, ampicillin preparations, praziquantel preparations, lysozyme chloride preparations, oxytetracycline hydrochloride preparations, spiramycin preparations, sodium nifurstyrene preparations, Pharmaceuticals for marine products such as lincomycin hydrochloride preparations, flumekine preparations, and glutathione preparations, vitamins such as vitamin C, vitamin B1, vitamin A, vitamin D, and vitamin E, and amino acids such as lysine, methionine, and histidine pigments such as beta-carotene, astaxanthin, and canthaxanthin; minerals such as calcium and silicic acid; trace metals;
  • the fish feed may have any shape and size depending on the type and size of the fish to be raised.
  • Fish feed includes, for example, powdered feed obtained by mixing and pulverizing dry raw materials, solidified feed obtained by solidifying powder (dry pellet), pasty feed containing moisture (moist pellet), live feed (fish fillet ) and the like.
  • powdered feed obtained by mixing and pulverizing dry raw materials
  • solidified feed obtained by solidifying powder (dry pellet)
  • pasty feed containing moisture miist pellet
  • live feed fish fillet
  • a method for producing fish feed first, general powdered feed for fish farming is mixed with plantaricin, nisin, or the cell preparation of the lactic acid bacteria. The mixture is shaped, for example extruded using a pasta machine or syringe. Finally, the molded product is dried at, for example, 60° C. to 65° C.
  • the colony-forming unit can be determined by an agar plate culture method.
  • the total amount (content ratio) of plantalysin, nisin, and the bacterial cell preparation of lactic acid bacteria in the fish feed is 0.001% by mass to 50% by mass, preferably 0.001% by mass to 30%, based on the weight of the feed. % by mass, more preferably 0.01% to 30% by mass, and even more preferably 0.01% to 10% by mass.
  • the total amount can be weighed with a balance or the like.
  • the feed containing plantaricin, nisin, or the lactic acid bacteria cell preparation may be fed once a day or divided into multiple times.
  • a feed containing plantaricin, nisin or the cell preparation of the lactic acid bacteria may be fed once every few days.
  • the period during which the feed containing plantalysin, nisin, or the above-mentioned lactic acid bacteria cell preparation is fed can be appropriately selected according to the type of target fish and the like.
  • the feed containing plantaricin, nisin, or the cell preparation of the lactic acid bacterium may be continuously fed during the entire cultivation (rearing) period, or may be fed only during a part of the period.
  • the total amount of plantalysin, nisin, and the bacterial cell preparation of lactic acid bacteria in the composition for injection is 0.001% by mass to 30% by mass, preferably 0.01% by mass, based on the weight of the entire composition for injection. It may be up to 10% by mass.
  • An embodiment of the present invention is the use of at least one selected from plantaricin and nisin in the manufacture of a fish breeding composition.
  • One embodiment of the present invention is the use of bacterial cells or cell cultures or extracts thereof having at least one selected from the plantarisin gene and the nisin gene in the manufacture of a fish breeding composition.
  • a method for raising fish organisms according to an embodiment of the present invention includes immersing fish organisms in breeding water containing at least one selected from plantaricin and nisin.
  • a method for breeding fish organisms according to an embodiment of the present invention is characterized in that breeding water containing bacterial cells or bacterial cell cultures having at least one selected from the plantarisin gene and the nisin gene, or an extract thereof. Including soaking fish organisms. Breeding water containing plantaricin, nisin and the cell preparation of the lactic acid bacterium may be supplied as a composition for breeding the fish organisms described above.
  • a method for raising fish organisms according to an embodiment of the present invention includes injecting at least one selected from plantaricin and nisin into fish organisms.
  • a method for breeding fish organisms according to one embodiment of the present invention is characterized in that bacteria having at least one selected from the plantaricin gene and the nisin gene, bacterial cells or bacterial cell cultures, or extracts thereof are fed to fish organisms. Including injecting. Plantaricin, nisin and the cell preparation of the lactic acid bacteria may be supplied as a composition for rearing the fish organisms described above.
  • the DNA sequence and peptide sequence of the plantaricin NC8 ⁇ gene possessed by NITE BP-03198 are shown in SEQ ID NOs: 16 and 17, and the DNA sequence and peptide sequence of the plantaricin NC8 ⁇ gene are shown in SEQ ID NOs: 18 and 19.
  • isolate D fermented galactose, fructose and melezitose, etc., but did not ferment glycerol, D-xylose, etc.
  • Isolate D showed no arginine dihydrolase activity and grew at 15°C. These properties were attributed to L. cerevisiae, whose attribution was suggested as a result of 16S rDNA partial nucleotide sequence analysis.
  • pentosus and L. Among L. plantarum, L. plantarum shows no fermentation of glycerol and D-xylose. Unlike L. pentosus, L. It matched the properties of plantarum. Therefore, isolate D was found to be a novel isolate belonging to Lactobacillus plantarum. Isolate D was internationally deposited as NITE BP-03201.
  • isolate E fermented galactose, fructose, ⁇ -methyl-D-mannoside and melezitose, etc., but did not ferment glycerol, D-xylose, etc.
  • Isolate E showed no arginine dihydrolase activity and grew at 15°C. These properties were attributed to L. cerevisiae, whose attribution was suggested as a result of 16S rDNA partial nucleotide sequence analysis.
  • pentosus and L Among L. plantarum, L. plantarum shows no fermentation of glycerol and D-xylose. Unlike L. pentosus, L. It matched the properties of plantarum. Therefore, isolate E was found to be a novel isolate belonging to Lactobacillus plantarum. The isolate E was internationally deposited as NITE BP-03202.
  • isolate F formed circular colonies. As shown in FIG. 6B, isolate F was positive for Gram staining. Tables 11 and 12 show the results of physiological/biochemical property tests and fermentability tests of isolate F. Isolate F was a non-motile, Gram-positive oomycoccus, did not form spores, was negative in catalase and oxidase reactions, and fermented glucose. These properties were consistent with those of the genus Lactococcus, for which the possibility of attribution was shown as a result of 16S rDNA partial nucleotide sequence analysis.
  • isolate F fermented ribose, galactose, maltose, etc., but did not ferment melibiose, melezitose, etc.
  • Isolate F grew at 15° C., grew in the presence of 4% NaCl, and exhibited arginine dihydrolase activity. These properties are attributed to Lactococcus lactis subsp. lactis. In particular, Lactococcus lactis subsp. hordniae. Therefore, isolate F is Lactococcus lactis subsp. lactis novel isolate.
  • the isolate F was internationally deposited as NITE ABP-03481.
  • bacterial solution 12 (about 10 5 cfu/mL) containing Lactococcus garvieae was added, and cultured with shaking at 25° C. for 8 hours.
  • 5 ⁇ L of bacterial culture solution 13 was added dropwise to TH agar medium 14 and cultured at 25° C. for 12 hours to observe whether colonies 15 of Lactococcus garvieae were formed. Antibacterial activity was judged to be present when 5 or fewer colonies were observed.
  • a cell preparation of lactic acid bacteria was prepared by the following method.
  • Example 8 Antibacterial activity evaluation test of synthetic plantaricin
  • the method of experiment 8 was the same as experiment 7, except that synthetic plantalysin was used instead of the cell preparation of lactic acid bacteria.
  • Each synthetic plantaricin was synthesized using a general method of organic synthesis.
  • Experiment 9 Antimicrobial Activity Evaluation Test of Nisin A Purified Product
  • the method of Experiment 9 was the same as Experiments 7 and 8, except that the nisin A purified product was used instead of the lactic acid bacteria cell preparation and synthetic plantaricin.
  • a nisin A solution was obtained by dissolving a purified nisin A product (manufactured by Sigma-Aldrich) in water and removing insoluble components by centrifugation.
  • the brains and kidneys of 10 fish in each test group that survived were sampled using a bacteria isolation loop, and the samples were seeded on an agar medium to isolate the bacteria.
  • the agar medium used was a Mueller-Hinton medium supplemented with a final concentration of 0.2% glucose.
  • the grown bacteria were placed on a slide, and a rabbit antiserum against type II streptococcus (provided by Professor Yoshida, University of Miyazaki) was used to confirm whether type II streptococci were detected. The results are shown in Table 18.

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PCT/JP2022/027146 2021-07-09 2022-07-08 魚類飼育用組成物、および魚病に対する予防または治療用組成物 Ceased WO2023282355A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN202280048254.3A CN117915781A (zh) 2021-07-09 2022-07-08 鱼类饲养用组合物和鱼病的预防或治疗用组合物
JP2023533204A JPWO2023282355A1 (https=) 2021-07-09 2022-07-08
EP22837769.3A EP4413867A4 (en) 2021-07-09 2022-07-08 FISH BREEDING COMPOSITION AND COMPOSITION FOR THE TREATMENT OR PREVENTION OF FISH DISEASES
KR1020247003675A KR20240034202A (ko) 2021-07-09 2022-07-08 어류 사육용 조성물, 및 어병에 대한 예방 또는 치료용 조성물
AU2022307811A AU2022307811A1 (en) 2021-07-09 2022-07-08 Fish rearing composition, and composition for treating or preventing fish diseases
US18/577,017 US20250099514A1 (en) 2021-07-09 2022-07-08 Fish rearing composition, and composition for preventing or treating fish diseases
CA3226285A CA3226285A1 (en) 2021-07-09 2022-07-08 Fish rearing composition, and composition for preventing or treating fish diseases

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JP2021114166 2021-07-09
JP2021-114166 2021-07-09

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