WO2023213141A1 - 一种靶向卵巢的多肽及其衍生物和应用 - Google Patents
一种靶向卵巢的多肽及其衍生物和应用 Download PDFInfo
- Publication number
- WO2023213141A1 WO2023213141A1 PCT/CN2023/081313 CN2023081313W WO2023213141A1 WO 2023213141 A1 WO2023213141 A1 WO 2023213141A1 CN 2023081313 W CN2023081313 W CN 2023081313W WO 2023213141 A1 WO2023213141 A1 WO 2023213141A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- modification
- polypeptide
- ovary
- ovarian
- derivative
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 124
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 108
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 101
- 230000032683 aging Effects 0.000 claims abstract description 40
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 36
- 210000000287 oocyte Anatomy 0.000 claims abstract description 32
- 230000002611 ovarian Effects 0.000 claims abstract description 31
- 208000017443 reproductive system disease Diseases 0.000 claims abstract description 29
- 208000035475 disorder Diseases 0.000 claims abstract description 23
- 238000002360 preparation method Methods 0.000 claims abstract description 20
- 239000003814 drug Substances 0.000 claims abstract description 19
- 229940079593 drug Drugs 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 15
- 201000004384 Alopecia Diseases 0.000 claims abstract description 14
- 230000002159 abnormal effect Effects 0.000 claims abstract description 13
- 238000000338 in vitro Methods 0.000 claims abstract description 12
- 235000013601 eggs Nutrition 0.000 claims abstract description 11
- 208000024963 hair loss Diseases 0.000 claims abstract description 11
- 230000003676 hair loss Effects 0.000 claims abstract description 11
- 238000001727 in vivo Methods 0.000 claims abstract description 9
- 201000010065 polycystic ovary syndrome Diseases 0.000 claims abstract description 9
- 230000006371 metabolic abnormality Effects 0.000 claims abstract description 8
- 230000027758 ovulation cycle Effects 0.000 claims abstract description 8
- 208000002500 Primary Ovarian Insufficiency Diseases 0.000 claims abstract description 7
- 230000001548 androgenic effect Effects 0.000 claims abstract description 7
- 206010036601 premature menopause Diseases 0.000 claims abstract description 7
- 208000017942 premature ovarian failure 1 Diseases 0.000 claims abstract description 7
- 230000004153 glucose metabolism Effects 0.000 claims abstract description 5
- 206010022489 Insulin Resistance Diseases 0.000 claims abstract description 4
- 230000037356 lipid metabolism Effects 0.000 claims abstract description 4
- 201000004535 ovarian dysfunction Diseases 0.000 claims abstract description 4
- 208000001072 type 2 diabetes mellitus Diseases 0.000 claims abstract description 4
- 201000001421 hyperglycemia Diseases 0.000 claims abstract description 3
- 230000004048 modification Effects 0.000 claims description 68
- 238000012986 modification Methods 0.000 claims description 68
- 210000001672 ovary Anatomy 0.000 claims description 42
- 230000008685 targeting Effects 0.000 claims description 22
- 230000000694 effects Effects 0.000 claims description 19
- 239000000523 sample Substances 0.000 claims description 19
- 229940088597 hormone Drugs 0.000 claims description 18
- 239000005556 hormone Substances 0.000 claims description 18
- 238000001514 detection method Methods 0.000 claims description 17
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 16
- 208000024891 symptom Diseases 0.000 claims description 14
- 230000005856 abnormality Effects 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 11
- 230000001850 reproductive effect Effects 0.000 claims description 10
- 210000004602 germ cell Anatomy 0.000 claims description 9
- 235000020958 biotin Nutrition 0.000 claims description 6
- 239000011616 biotin Substances 0.000 claims description 6
- 229960002685 biotin Drugs 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 6
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 6
- 208000002874 Acne Vulgaris Diseases 0.000 claims description 5
- 208000011707 Ovulation disease Diseases 0.000 claims description 5
- 206010000496 acne Diseases 0.000 claims description 5
- 239000002502 liposome Substances 0.000 claims description 5
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- 208000004930 Fatty Liver Diseases 0.000 claims description 4
- 206010019708 Hepatic steatosis Diseases 0.000 claims description 4
- 206010020112 Hirsutism Diseases 0.000 claims description 4
- 208000008589 Obesity Diseases 0.000 claims description 4
- 208000001132 Osteoporosis Diseases 0.000 claims description 4
- 230000021736 acetylation Effects 0.000 claims description 4
- 238000006640 acetylation reaction Methods 0.000 claims description 4
- 238000005576 amination reaction Methods 0.000 claims description 4
- 150000001540 azides Chemical class 0.000 claims description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 4
- 239000000969 carrier Substances 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 208000010706 fatty liver disease Diseases 0.000 claims description 4
- 230000013595 glycosylation Effects 0.000 claims description 4
- 238000006206 glycosylation reaction Methods 0.000 claims description 4
- 238000001948 isotopic labelling Methods 0.000 claims description 4
- 239000002105 nanoparticle Substances 0.000 claims description 4
- 235000020824 obesity Nutrition 0.000 claims description 4
- 230000026731 phosphorylation Effects 0.000 claims description 4
- 238000006366 phosphorylation reaction Methods 0.000 claims description 4
- 102000040430 polynucleotide Human genes 0.000 claims description 4
- 108091033319 polynucleotide Proteins 0.000 claims description 4
- 239000002157 polynucleotide Substances 0.000 claims description 4
- -1 small molecule compound Chemical class 0.000 claims description 4
- 231100000240 steatosis hepatitis Toxicity 0.000 claims description 4
- 206010027304 Menopausal symptoms Diseases 0.000 claims description 3
- 231100000360 alopecia Toxicity 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 3
- 230000003054 hormonal effect Effects 0.000 claims description 3
- 239000003504 photosensitizing agent Substances 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- HKAVADYDPYUPRD-UHFFFAOYSA-N 1h-pyrazine-2-thione Chemical compound SC1=CN=CC=N1 HKAVADYDPYUPRD-UHFFFAOYSA-N 0.000 claims description 2
- 235000000638 D-biotin Nutrition 0.000 claims description 2
- 239000011665 D-biotin Substances 0.000 claims description 2
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims description 2
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims description 2
- 208000034189 Sclerosis Diseases 0.000 claims description 2
- GYDJEQRTZSCIOI-UHFFFAOYSA-N Tranexamic acid Chemical compound NCC1CCC(C(O)=O)CC1 GYDJEQRTZSCIOI-UHFFFAOYSA-N 0.000 claims description 2
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims description 2
- 239000004480 active ingredient Substances 0.000 claims description 2
- 230000029936 alkylation Effects 0.000 claims description 2
- 238000005804 alkylation reaction Methods 0.000 claims description 2
- 230000009435 amidation Effects 0.000 claims description 2
- 238000007112 amidation reaction Methods 0.000 claims description 2
- 239000000427 antigen Substances 0.000 claims description 2
- 102000036639 antigens Human genes 0.000 claims description 2
- 108091007433 antigens Proteins 0.000 claims description 2
- 229920002988 biodegradable polymer Polymers 0.000 claims description 2
- 239000004621 biodegradable polymer Substances 0.000 claims description 2
- 230000006315 carbonylation Effects 0.000 claims description 2
- 238000005810 carbonylation reaction Methods 0.000 claims description 2
- 230000021523 carboxylation Effects 0.000 claims description 2
- 238000006473 carboxylation reaction Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 108010048367 enhanced green fluorescent protein Proteins 0.000 claims description 2
- 230000032050 esterification Effects 0.000 claims description 2
- 238000005886 esterification reaction Methods 0.000 claims description 2
- 210000001808 exosome Anatomy 0.000 claims description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims description 2
- 230000033444 hydroxylation Effects 0.000 claims description 2
- 238000005805 hydroxylation reaction Methods 0.000 claims description 2
- 238000002372 labelling Methods 0.000 claims description 2
- 239000003446 ligand Substances 0.000 claims description 2
- 230000011987 methylation Effects 0.000 claims description 2
- 238000007069 methylation reaction Methods 0.000 claims description 2
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 claims description 2
- USRGIUJOYOXOQJ-GBXIJSLDSA-N phosphothreonine Chemical compound OP(=O)(O)O[C@H](C)[C@H](N)C(O)=O USRGIUJOYOXOQJ-GBXIJSLDSA-N 0.000 claims description 2
- DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 claims description 2
- 238000010791 quenching Methods 0.000 claims description 2
- 230000000171 quenching effect Effects 0.000 claims description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 2
- 238000007363 ring formation reaction Methods 0.000 claims description 2
- 239000004615 ingredient Substances 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 12
- 238000004321 preservation Methods 0.000 abstract 2
- 208000021959 Abnormal metabolism Diseases 0.000 abstract 1
- 206010002659 Anovulatory cycle Diseases 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 49
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical group C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 23
- 241000699666 Mus <mouse, genus> Species 0.000 description 13
- 238000011282 treatment Methods 0.000 description 13
- 229960003473 androstanolone Drugs 0.000 description 12
- 230000004720 fertilization Effects 0.000 description 11
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 10
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 8
- 210000003684 theca cell Anatomy 0.000 description 8
- 239000003098 androgen Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 206010042573 Superovulation Diseases 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 230000016087 ovulation Effects 0.000 description 6
- 230000004913 activation Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- 150000002632 lipids Chemical class 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 238000009825 accumulation Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 229940011871 estrogen Drugs 0.000 description 4
- 239000000262 estrogen Substances 0.000 description 4
- 230000012173 estrus Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 230000004807 localization Effects 0.000 description 4
- 238000010172 mouse model Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 239000000186 progesterone Substances 0.000 description 4
- 229960003387 progesterone Drugs 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- 210000002536 stromal cell Anatomy 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 3
- 206010033128 Ovarian cancer Diseases 0.000 description 3
- 206010061535 Ovarian neoplasm Diseases 0.000 description 3
- 229930040373 Paraformaldehyde Natural products 0.000 description 3
- 102000001307 androgen receptors Human genes 0.000 description 3
- 108010080146 androgen receptors Proteins 0.000 description 3
- 229940030486 androgens Drugs 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000003937 drug carrier Substances 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 210000002570 interstitial cell Anatomy 0.000 description 3
- 238000001990 intravenous administration Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920002866 paraformaldehyde Polymers 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 238000013268 sustained release Methods 0.000 description 3
- 239000012730 sustained-release form Substances 0.000 description 3
- 238000001308 synthesis method Methods 0.000 description 3
- 210000004340 zona pellucida Anatomy 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 2
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 241000282553 Macaca Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 230000002710 gonadal effect Effects 0.000 description 2
- 210000002503 granulosa cell Anatomy 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 206010020718 hyperplasia Diseases 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000007918 intramuscular administration Methods 0.000 description 2
- 238000007912 intraperitoneal administration Methods 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 208000015124 ovarian disease Diseases 0.000 description 2
- 230000000136 postovulatory effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000010532 solid phase synthesis reaction Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- QRZUPJILJVGUFF-UHFFFAOYSA-N 2,8-dibenzylcyclooctan-1-one Chemical compound C1CCCCC(CC=2C=CC=CC=2)C(=O)C1CC1=CC=CC=C1 QRZUPJILJVGUFF-UHFFFAOYSA-N 0.000 description 1
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 208000031648 Body Weight Changes Diseases 0.000 description 1
- 208000020084 Bone disease Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 208000017701 Endocrine disease Diseases 0.000 description 1
- 206010014733 Endometrial cancer Diseases 0.000 description 1
- 206010014759 Endometrial neoplasm Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 241000282577 Pan troglodytes Species 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 206010047486 Virilism Diseases 0.000 description 1
- 150000001345 alkine derivatives Chemical class 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000004579 body weight change Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000006482 condensation reaction Methods 0.000 description 1
- 210000001771 cumulus cell Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000001158 estrous effect Effects 0.000 description 1
- 230000006408 female gonad development Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000008217 follicular development Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 208000018914 glucose metabolism disease Diseases 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000001361 intraarterial administration Methods 0.000 description 1
- 238000007917 intracranial administration Methods 0.000 description 1
- 238000007913 intrathecal administration Methods 0.000 description 1
- 238000007914 intraventricular administration Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000794 masculinization Toxicity 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/10—Peptides having 12 to 20 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
- A61K49/0056—Peptides, proteins, polyamino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
- A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0609—Oocytes, oogonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
Definitions
- the invention belongs to the field of medical biology and relates to an ovary-targeting polypeptide and its derivatives and applications.
- the ovary is a very important reproductive organ in women. It is composed of germ cells (oocytes or eggs) and somatic cells (granulosa cells, theca cells and stromal cells). Their interaction determines the function of the ovary, that is, the secretion of secretions affects follicle development, The menstrual/estrous cycle and hormones necessary to maintain endocrine health are associated with the production of mature eggs for fertilization to produce offspring. Abnormal ovarian function can lead to a series of ovarian reproductive diseases such as premature ovarian failure (POF), polycystic ovary syndrome (Polycystic Ovary Syndrome, PCOS), ovulation disorders and even ovarian cancer (OC).
- POF premature ovarian failure
- PCOS polycystic Ovary Syndrome
- OC ovarian cancer
- Ovarian reproductive diseases can lead to hormone disorders in the body, showing a series of abnormalities in glucose and lipid metabolism such as obesity, insulin resistance, fatty liver, osteoporosis, etc.; reproductive abnormalities such as excessive activation of follicles, reduced ovulation, and infertility; and aging phenomena such as early menopausal symptoms. wait.
- High levels of androgens in females can lead to a series of androgenic phenotypes such as hirsutism, acne, alopecia, etc.
- High levels of estrogen can lead to the occurrence of a series of reproductive cancer diseases such as ovarian cancer, breast cancer, and endometrial cancer, seriously harming women's physical and mental health. Health and life safety.
- the window period for fertilization after oocyte maturation is very limited.
- the optimal time for fertilization and development in mice is within 12 hours after ovulation, while in humans it is within 4-12 hours after ovulation or egg retrieval.
- the discharged oocytes enter the aging stage and eventually undergo apoptosis and are unable to undergo fertilization.
- inhibiting post-ovulatory aging in mammals can improve and increase the fertilization rate and fertility rate.
- treatments for diseases related to ovarian germ cells and endocrine disorders mainly include drug treatment, surgical treatment and assisted reproductive technology.
- drug treatment the biggest problem is that it cannot be targeted. Drugs act on the whole body and may cause various side effects on other tissues and organs of the body.
- surgical treatment and assisted reproductive technology in addition to the low success rate, there is also a lack of post-treatment results. Clear non-invasive real-time observation of ovarian development. Therefore, it is of great social significance and broad economic market to screen out polypeptides that can specifically target the ovary in vivo, study their role in ovarian reproductive diseases, and serve as drug carriers for targeted treatment of ovarian reproductive diseases.
- the purpose of the present invention is to provide the functions and applications of an ovary-targeting polypeptide and its derivatives.
- the present invention has discovered the following sequence polypeptides, which can specifically target the ovaries and alleviate a variety of ovary-related reproductive diseases, metabolic abnormalities and symptoms caused by aging caused by hormone disorders.
- polypeptide sequence comprising the sequence represented by formula M1-Za-M2 or the sequence represented by Za, wherein:
- M1 and M2 each independently have single or multiple amino acid polypeptide segments or do not exist;
- Za is Tyr-Leu-X1-X2-X3-X4-Gly-Ala-X5-X6-Pro-X7-Pro-Asp-X8-Leu-Glu-Pro-Thr
- X1 is Asn or Tyr or Asp or does not exist
- X2 is Asn or Gln or His or Pro or Ser or does not exist
- X3 is Gly or Trp or does not exist
- X4 is Leu or does not exist
- X5 is Pro or Ser
- X6 is Ala or Val
- X7 is Tyr or Ser or Ala
- X8 is Pro or Thr
- Za is selected from one of:
- Another aspect of the present invention provides a derivative of an ovary-targeting polypeptide, which is a product obtained by conventional modification of the amino acid side chain group, amino terminus, and carboxyl terminus of the polypeptide, or is a product for connecting to the polypeptide.
- Products obtained from tags for polypeptide or protein detection or purification, or products modified by isotope labeling include fluorescent group modification, phosphorylation modification, cyclization modification of disulfide bonds, and biotin labeling Modification, photosensitizer, azide modification, PEG modification, methylation modification, fluorescence quenching group modification, protein coupling modification, small molecule compound modification, amination modification, amidation modification, hydroxylation modification, carboxylation modification, Carbonylation modification, alkylation modification, acetylation modification, esterification modification, Glycosylation modification;
- the tag is His 6 , GST, EGFP, MBP, Nus, HA, IgG, FLAG, c-Myc or ProfinityeXact.
- modifications at the amino terminus and carboxyl terminus are selected from the group consisting of acetylation modification at the N-terminus of the polypeptide and amination modification at the C-terminus.
- modification of the side chain is selected from modifications on the R group of the amino acid side chain in the polypeptide.
- the fluorescent dye used in the modification of the fluorescent group is selected from AMCA, FITC, Rhodamine, Cy3, Cy5, Cy5.5, Cy7, AIE, and ICG. This modification can be used for fluorescence detection.
- the phosphorylation modification is selected from one or a combination of p-Ser, p-Thr, and p-Tyr.
- glycosylation modification is selected from one or a combination of more of Ser, Asn, Thr, and Tyr.
- nitrolation modification is selected from one or a combination of more than Tyr.
- biotin label is selected from the group consisting of D-biotin, biotin hydrazide, photosensitive biotin and biotin-dUTP.
- the photosensitizer modification can be used to prepare photosensitive preparations.
- the azide modification can be used in secondary ligation reactions.
- the PEG modification can be used to prepare pharmaceutical carriers.
- the isotope used in the isotope labeling is selected from one or more of 13C, 14C, 14N, 15N, 2H, 3H, 18O, 32P, 32S, 34S, 35S, 36S, 35Cl, 37Cl, 125I, and 131I. combination of species.
- Another aspect of the present invention provides a polynucleotide encoding the above-mentioned polypeptide or a derivative thereof.
- Yet another aspect of the present invention provides a vector comprising the above polynucleotide.
- Yet another aspect of the present invention provides a host cell transfected with the above vector.
- Another aspect of the present invention provides the use of the polypeptide of the present invention or a derivative thereof in the preparation of an ovary-targeting detection reagent, an ovary-targeting drug, or an ovary-targeting tool.
- the tool is selected from carriers or preparations, such as carriers, preparations or kits that achieve targeting effects by coupling the polypeptides of the present invention or derivatives thereof, preparations such as liposomes, nanoparticles, etc.
- Another aspect of the present invention provides the use of the above-mentioned polypeptide or derivatives thereof in the preparation of drugs or detection reagents capable of targeting the ovary.
- medicines and detection reagents include the above-mentioned polypeptides and derivatives thereof as skeletons, and detection products prepared as antigens, such as detection reagents, test kits, and test strips.
- Another aspect of the present invention provides an ovary-targeted vector.
- the above-mentioned polypeptide and its derivatives as a backbone can be used as tools for targeted treatment of ovary-related reproductive diseases.
- the carrier is selected from liposomes, nanoparticles, exosomes, biodegradable polymer substances, and antibody-ligands.
- the carrier contains components that can treat ovarian-related reproductive diseases.
- polypeptides and their derivatives as skeletons can be synthesized independently under general chemical laboratory conditions, can be synthesized through gene expression of recombinant proteins containing the above polypeptides, or can be industrially synthesized by commercial reagent companies using solid-phase methods.
- Polypeptides, different amino acids on the resin are directed to synthesize amino acid chains through condensation reactions. Derivatives of polypeptides are labeled with modifying groups after the amino acids are connected.
- the present invention provides a drug targeting the ovary, which is a conjugate of the above-mentioned polypeptide and derivatives thereof as a skeleton and active ingredients.
- the polypeptide and its derivatives as a skeleton can treat reproductive, metabolic and aging-related diseases caused by germ cell abnormalities and hormone disorders by targeting the ovaries.
- the present invention provides a polypeptide probe targeting the ovary.
- the polypeptide probe is the above-mentioned polypeptide or a derivative thereof as a skeleton, and is prepared by coupling with a fluorescent dye through an organic chemical reaction.
- the fluorescent dye is selected from the group consisting of AIE, Cy5, Cy7, and ICG.
- Another aspect of the present invention provides the use of the polypeptide in the preparation of drugs or reagents for treating ovarian-related reproductive diseases.
- the ovary-related reproductive diseases are selected from ovary-related reproductive diseases caused by germ cell abnormalities or hormonal disorders.
- the hormonal disorder is an excess of male hormones above normal levels.
- the ovarian-related reproductive diseases caused by germ cell abnormalities are ovarian-related reproductive diseases caused by aging of oocytes and or sclerosis of the transparent bag of oocytes, preferably ovarian-related reproductive diseases caused by the germ cell abnormalities.
- Diseases are selected from abnormal oocyte numbers, decreased oocyte quality, and egg aging.
- the ovary-related reproductive diseases caused by hormone disorders include menstrual cycle disorders, polycystic ovary syndrome, androgenic phenotype, hair loss, metabolic abnormalities, ovarian insufficiency, premature ovarian failure, and ovulation disorders.
- the metabolic abnormality caused by hormone disorder is selected from abnormal glucose metabolism and abnormal lipid metabolism, preferably hyperglycemia, insulin resistance, obesity, fatty liver, and osteoporosis.
- the androgenic phenotype is selected from acne, hirsutism, and alopecia.
- the aging and related symptoms caused by hormone disorders include perimenopause, menopausal syndrome, aging, acne, acne, hirsutism, and hair loss.
- Another aspect of the present invention provides the use of the polypeptide in preparing drugs or reagents for preventing and treating aging of oocytes in vivo or in vitro.
- the present invention provides an assisted reproductive product, which includes the polypeptide of the present invention or its derivatives.
- assisted reproduction product is a protective solution for oocytes and/or fertilized eggs during assisted reproduction.
- Another aspect of the present invention provides the use of the above-mentioned assisted reproduction products in preparing a protective solution for preserving oocytes and/or fertilized eggs during the process of assisted reproduction.
- the candidate polypeptides provided by the present invention and derivatives thereof as skeletons can specifically target the ovaries in vivo and in vitro.
- the candidate polypeptides and derivatives thereof as skeletons provided by the present invention can be used as modified polypeptides to enhance the targeting and intervention and therapeutic effects of drugs or drug carriers on the ovary.
- the candidate polypeptides and derivatives thereof as skeletons provided by the present invention are easy to synthesize and modify, and are low-cost, so they have broad application prospects.
- the candidate polypeptides provided by the present invention and their derivatives as skeletons provide a wide range of preparation strategies for diagnostic and therapeutic compounds or drugs for their practical applications.
- the candidate polypeptides and derivatives thereof as skeletons provided by the present invention are suitable for targeted treatment of ovarian-related reproductive diseases, and are also suitable as research and observation tools in experimental animals for modeling ovarian diseases.
- Figure 1 is a diagram showing the effect of targeting the ovary in vivo by the polypeptide connected to ICG.
- the figure shows the fluorescence signal distribution and results of the ovaries and uterus of mice in the PBS group, ICG group and ICG-linked peptide group.
- Figure 2 is a diagram showing the effect of targeting the ovary in vivo with the polypeptide connected to AIE.
- the figure shows the fluorescence signal distribution and results of the four groups of mouse ovaries: PBS group, AIE empty particle group, AIE connected peptide group, and AIE connected to other peptide groups.
- Figure 3 shows the localization map of the ovarian targeting of the polypeptide connected to AIE in vivo.
- the figure shows the fluorescence signal localization of the mouse ovaries in four groups: PBS group, AIE empty particle group, AIE connected peptide group and AIE connected to other peptide groups.
- arrows indicate theca cells and asterisks indicate interstitial cells.
- Figure 4 shows the alleviation effect of ovarian-targeted peptides on hyperandrogen-induced abnormal follicular development, estrous cycle disorders, androgen phenotype and blood glucose abnormalities in mice induced by hyperandrogen-dihydrotestosterone (DHT).
- Figure 4A is the morphological observation of the ovaries of mice in the control group, DHT group and DHT+ovarian targeting peptide group.
- Figure 4B shows the number of estrous days in the three groups of mice in ten days.
- Figure 4C shows the distance between the anogenital areas of mice after 60 days of modeling (the distance between the anogenital areas of males is larger).
- Figure 4D shows the changes in blood glucose after oral administration of glucose to mice 60 days after modeling.
- Figure 5 shows the alleviating effect of ovarian-targeted peptides on weight changes caused by hyperandrogen in a mouse model induced by hyperandrogen-dihydrotestosterone (DHT).
- Figure 5A shows changes in body weight
- Figure 5B shows changes in weight gain.
- Figure 5C shows the change in gonadal fat weight
- Figure 5D shows the change in lean body mass except fat.
- Figure 6 shows the effect of ovarian-targeted polypeptide on the hair loss on the neck and back of mice caused by hyperandrogen in a mouse model induced by hyperandrogen-dihydrotestosterone (DHT).
- Figure 6A is the observation of hair loss on the back of the neck and back of mice in the control group, DHT group and DHT+ovarian-targeting peptide group.
- Figure 6B is an immunofluorescence image of the expression of androgen receptor AR in the neck and back skin of three groups of mice.
- Figure 7 shows the improvement effect of targeted peptides on oocyte fragmentation and degeneration, zona pellucida hardening and in vitro fertilization caused by aging in the post-ovulatory aging model of oocytes.
- Figure 7A shows the degradation and fragmentation rates of the control group, aging group and targeted peptide group after aging for 12 hours.
- Figure 7B shows the hardening levels of the zona pellucida in the control group, aging group and targeted peptide group after 12 hours of aging.
- Figure 7C shows the in vitro fertilization rate after 12 hours of in vitro aging in the control group, aging group and targeted peptide group.
- Figure 8 shows the changes in serum estrogen and progesterone levels in OCN15KO mice compared with WT mice in the superexcretion model.
- the left picture of Figure 8 shows the changes in estrogen levels during superovulation.
- the right picture of Figure 8 shows the changes in progesterone levels during superovulation.
- Figure 9 shows the effect of ovarian-targeted polypeptide on the accumulation of lipid droplets in the mouse liver caused by hyperandrogen in a mouse model induced by hyperandrogen-dihydrotestosterone (DHT).
- DHT hyperandrogen-dihydrotestosterone
- Figure 10 is a sequence comparison diagram of metabolins derived from different species.
- subject means, but are not limited to, humans, mice, rats, chimpanzees, macaques, cattle, sheep, wild boars, moles, dogs. Also covered are tissues, cells and their progeny of biological entities obtained in vivo or cultured in vitro.
- administration includes oral administration, topical contact, administration as a suppository, intravenous, intraperitoneal, intramuscular, intralesional, intrathecal, intranasal or subcutaneous administration to a subject.
- Administration is by any route, including parenteral and transmucosal (eg, buccal, sublingual, transpalatal, transgingival, nasal, transvaginal, transrectal, or transdermal).
- Parenteral administration includes, for example, intravenous, intramuscular, intraarterial, intradermal, subcutaneous, intraperitoneal, intraventricular and intracranial.
- Other delivery modes include, but are not limited to, the use of liposome formulations, intravenous infusion, transdermal patches, etc.
- treatment refers to a method used to obtain beneficial or desired results including, but not limited to, therapeutic benefits.
- Therapeutic benefit means any treatment-related improvement in one or more diseases, conditions, or symptoms being treated, or any treatment-related effect on one or more diseases, conditions, or symptoms being treated.
- prevention refers to the prevention, including but not limited to A method that has beneficial or desired results, including preventive benefits.
- the composition may be administered to a subject at risk of developing a particular disease, condition, or symptom, or to a subject who reports one or more physiological symptoms of a disease, even if the disease, condition, or symptom It may not show up yet.
- therapeutically effective amount refers to an amount of a peptide or composition sufficient to achieve a beneficial or desired result.
- the therapeutically effective amount may vary depending on one or more of the following: the subject and disease condition being treated, the weight and age of the subject, the severity of the disease condition, the mode of administration, etc., which can be readily determined by one of ordinary skill in the art. Determined.
- the specific amount may vary depending on one or more of the following: the specific agent selected, the target cell type, the location of the target cells in the subject, the dosing regimen to be followed, whether it is administered in combination with other compounds, and the timing of administration. and the physical delivery systems that carry it.
- polypeptide sequence is as follows:
- the above polypeptides are synthesized using conventional solid phase synthesis or liquid phase synthesis methods.
- the solid-phase polypeptide synthesis method is used to react from the C-terminal amino acid to the N-terminal. After resin activation, amino acid linkage, elution protection, detection and other steps, the amino acid linkage is completed one by one, and then excessive ether is used to precipitate and centrifuge, and the crude peptide is purified by HPLC. Afterwards, mass spectrometry analysis was carried out and freeze-dried in liquid nitrogen for later use.
- the peptides are all isolated peptides.
- SEQ ID NO.1 is mouse metabolin
- SEQ ID NO.2 is rat metabolin
- SEQ ID NO.3 is human metabolin
- SEQ ID NO.4 is chimpanzee metabolin
- SEQ ID NO.5 is macaque.
- Metabolin SEQ ID NO.6 is bovine metabolin
- SEQ ID NO.7 is sheep metabolin
- SEQ ID NO.8 is wild boar metabolin
- SEQ ID NO.9 is mole metabolin
- SEQ ID NO.10 is Dog metabolin (shown in Figure 10).
- This embodiment provides a polypeptide probe, which uses the polypeptide prepared in Example 1 and combines it with ICG/AIE through an organic chemical reaction.
- the specific method is commissioned synthesis or self-synthesis.
- the polypeptide is connected to ICG or AIE through click chemistry to complete the probe preparation.
- the linker is a universal DBCO.
- ICG/AIE with activation functional groups include amino NH 2 , carboxyl COOH, activated lipid NHS, maleimide MAL, sulfhydryl SH, azide N 3 , alkyne ALK, and then combined with Example 1
- the prepared polypeptide is coupled to obtain a polypeptide ICG/AIE probe.
- Example 3 Detection of the ability of polypeptide ICG probes to target mouse ovaries
- mice were injected into the tail vein of the polypeptide ICG probe (containing SEQ ID NO.1) solution prepared in Example 2, ICG solution (2mM) and PBS. Each mouse was injected with 100 ⁇ L. After 24 hours, the solution was measured under a small animal imager. detection.
- the polypeptide of the present invention has a specific response to the ovary and can achieve the effect of targeting the ovary in the body.
- Example 4 Detection of the ability of polypeptide AIE probes to target mouse ovaries
- mice were injected into the tail vein of the polypeptide AIE probe (containing SEQ ID NO.1) prepared in Example 2, the scrambled polypeptide AIE probe, AIE empty particles and PBS. Each mouse was injected with 100 ⁇ L. After 24 hours, the mice were Detection under animal imager.
- the polypeptide of the present invention has a specific response to the ovary and can achieve the effect of targeting the ovary in the body.
- polypeptide of the present invention has a specific response to the ovary, and its modification will not affect its targeting effect. This indicates that the polypeptide of the present invention can be used to prepare drugs targeting the ovary. Testing reagents or preparations, etc.
- Example 5 Targeting of polypeptides to mouse ovaries
- the ovaries of the four groups of mice in Example 4 were fixed with 4% PFA, frozen sections (10 ⁇ m), stained for cell nuclei (DAPI), and the fluorescence localization was observed under a laser confocal microscope.
- the peptide AIE probe set (OCN15-AIE) mainly targets mouse ovarian stromal cells and theca cells.
- the mediating polypeptide provided by the present invention can be used for the purpose of ovarian targeting, especially targeting ovarian stromal cells and theca cells in the ovary. Modifications on the polypeptide will not affect the function of the polypeptide.
- Example 5 can prove that OCN15 targets ovarian stromal cells and theca cells. Combined with the results of the following examples, it can be expected to be applied to ovarian diseases related to abnormalities of these two cells, such as the thickening of theca cells caused by hyperandrogen. and interstitial cell hyperplasia, ovulation disorders caused by theca cell thickening and interstitial hyperplasia, ovarian insufficiency caused by ovulation disorders, menstrual cycle disorders and polycystic ovary syndrome, etc.
- ovarian diseases related to abnormalities of these two cells such as the thickening of theca cells caused by hyperandrogen. and interstitial cell hyperplasia, ovulation disorders caused by theca cell thickening and interstitial hyperplasia, ovarian insufficiency caused by ovulation disorders, menstrual cycle disorders and polycystic ovary syndrome, etc.
- Example 6 Effects of ovary-targeted polypeptides on reproductive and metabolic symptoms induced by hyperandrogen in mice
- control group DHT
- DHT+OCN15 OCT15 is the polypeptide shown in SEQ ID NO.1
- the blood sugar test results are shown in Figure 4D.
- the peak of blood sugar should be 15 minutes after oral glucose.
- the control group has dropped, but the DHT group is still very high, with a difference of 3 stars from the control group.
- OCN15 treatment There was a significant decrease compared with the DHT alone group and no difference with the control group, indicating that OCN15 normalized the abnormal blood glucose induced by DHT at 30 minutes.
- FIG. 5 A and B illustrate that OCN15 can alleviate the increase in body weight and body weight gain of mice caused by DHT to a certain extent, mainly reducing the weight of gonadal fat in mice (Figure 5C).
- the lean body mass of mice treated with OCN15 combined with DHT increased.
- Lean body mass is mainly the weight of bones, indicating that OCN15 can alleviate osteoporosis caused by hormone disorders.
- OCN15 can be applied to ovarian-related reproductive diseases caused by hyperandrogen, including menstrual cycle disorders, polycystic ovary syndrome, and androgenic phenotypes, as well as metabolic abnormalities caused by hormone disorders such as abnormal glucose metabolism, obesity, and bone disease. Loose quality.
- OCN15 can be applied to related symptoms caused by hyperandrogen such as female hair loss.
- Example 7 Effect of ovarian-targeted polypeptide on aging of oocytes after ovulation
- Wild-type female mice were intraperitoneally injected with 5IU PMSG (Pregnant Mare Serum Gonadotropin), and 48 hours later, 5IU hCG (Human Chorionic Gonadotropin) was intraperitoneally injected to perform superovulation. After 14 hours, the oocytes were collected, hyaluronidase was used to remove cumulus cells, and they were randomly divided into Aging group and Aging+OCN15 group and cultured and aged in M16 culture medium. The aging time of the control group (Control) was 0 hours, the aging time of the Aging group was 12 hours, and the Aging+OCN15 group was aged for 12 hours in M16 (1 ⁇ g/mL) containing peptides.
- 5IU PMSG Pregnant Mare Serum Gonadotropin
- 5IU hCG Human Chorionic Gonadotropin
- OCN15 can improve the fragmentation and degradation of oocytes caused by aging in vitro ( Figure 7A).
- OCN15 can improve the hardening of the zona pellucida caused by aging of oocytes in vitro ( Figure 7B).
- OCN15 can improve the low fertilization rate caused by in vitro aging of oocytes, and is even close to the fertilization rate at 0 hours of aging, with no significant difference.
- OCN15 can be used to protect oocyte quality, delay its aging process, and improve the low fertilization rate caused by aging. It can be used in the field of assisted reproduction to improve the storage time and quality of oocytes and fertilized eggs. At the same time, it can be expected that because it has the effect of improving the aging of oocytes in vitro, it can be used for diseases caused by abnormalities in oocytes caused by aging, such as abnormal oocyte number, decreased oocyte quality, and egg aging.
- the mediating polypeptide provided by the present invention can be used to treat ovarian-related reproductive diseases, metabolic abnormalities, hair loss and other symptoms caused by hormone disorders that increase the fertilization window of oocytes.
- Example 8 Effects of targeted ovarian polypeptide knockout on serum hormone levels in mice during superovulation
- Wild-type female mice and OCN15KO mice were intraperitoneally injected with 5IU PMSG, and 48 hours later, 5IU hCG was intraperitoneally injected for superovulation. Mice were sacrificed before injection, PMSG24h, PMSG48h, and hCG12h, and serum was taken to detect sex hormone levels.
- Example 9 Effect of ovary-targeted polypeptide on hyperandrogen-induced liver lipid droplet accumulation in mice
- Example 6 The method for inducing the mouse PCOS model with hyperandrogenic DHT is shown in Example 6. After the modeling was completed, mouse livers were removed, fixed in 4% paraformaldehyde (PFA) overnight, dehydrated through alcohol gradients, embedded in paraffin, sectioned (5um), and stained with HE for morphological observation.
- PFA paraformaldehyde
- the ovary-targeting polypeptide of the present invention can alleviate the accumulation of lipid droplets in the liver of mice induced by hyperandrogen, and can be used to treat fatty liver.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Diabetes (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Epidemiology (AREA)
- Reproductive Health (AREA)
- Biophysics (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Gynecology & Obstetrics (AREA)
- Toxicology (AREA)
- Child & Adolescent Psychology (AREA)
- Emergency Medicine (AREA)
Abstract
提供了一种靶向卵巢的多肽、所述多肽的衍生物及其在制备治疗卵巢相关生殖疾病、防治卵母细胞在体内或体外老化的药物或试剂中的用途,所述疾病包括月经周期紊乱、多囊卵巢综合征、雄化表型、毛发脱落、代谢异常、卵巢功能不全、卵巢早衰、排卵障碍、糖代谢异常、脂代谢异常,优选为高血糖、胰岛素抵抗。此外,所述多肽有望在辅助生殖领域延长卵母细胞或受精卵的保存时间和保存质量。
Description
本发明属于医学生物学领域,涉及一种卵巢靶向多肽及其衍生物和应用。
卵巢是女性非常重要的生殖器官,由生殖细胞(卵母细胞或卵子)和体细胞(颗粒细胞、膜细胞和基质细胞)组成,它们的相互作用决定了卵巢的功能,即分泌对卵泡发育、月经/发情周期和维持内分泌健康所必须的激素与产生成熟的卵子以供受精来产生后代。卵巢功能的异常会导致一系列卵巢生殖疾病如卵巢早衰(Premature Ovarian Failure,POF)、多囊卵巢综合症(Polycystic Ovary Syndrome,PCOS)、排卵障碍甚至卵巢癌(Ovarian Cancer,OC)的发生。
卵巢生殖疾病会导致机体激素紊乱,表现出一系列糖脂代谢异常如肥胖、胰岛素抵抗、脂肪肝、骨质疏松等;生殖异常如卵泡过度激活、排卵减少、不孕;衰老现象如更年期症状提前等。雌性体内高雄激素会导致一系列雄化表型如多毛、痤疮、脱发等,而高雌激素会导致一系列如卵巢癌、乳腺癌、子宫内膜癌等生殖肿瘤疾病的多发,严重危害女性身心健康和生命安全。
卵母细胞成熟后受精的窗口期非常有限。例如,小鼠最佳受精和发育的时间是排卵后不12小时内,而人类在排卵或取卵后4-12小时内。随时间推移,排出的卵母细胞进入老化阶段,最终凋亡不能进行受精。同时,在辅助生殖干预中,抑制哺乳动物排卵后老化可改善与提高受精率与生育率。
目前,针对卵巢生殖细胞和内分泌紊乱相关疾病的治疗主要有药物治疗、手术治疗和辅助生育技术。针对药物治疗方面,最大的问题是无法靶向治疗,药物作用于全身,可能对机体其他组织器官产生各种副作用;针对手术治疗和辅助生殖技术方面,除了成功率较低以外,还缺乏治疗后对卵巢发育情况较为清晰的无创实时观察。因此,筛选出能够在体内特异性靶向卵巢的多肽,研究其在卵巢生殖疾病中的作用以及作为靶向治疗卵巢生殖疾病的药物载体,具有重要的社会意义和广阔的经济市场。
发明内容
就上述背景技术中所提出的针对卵巢生殖疾病的治疗瓶颈问题,本发明的目的在于提供一种卵巢靶向多肽及其衍生物的功能和应用。
本发明发现了以下序列多肽,这些多肽能特异性靶向卵巢并缓解多种因为激素紊乱导致的卵巢相关生殖疾病、代谢异常和衰老引发的症状。
本发明一个方面提供了一种多肽,所述多肽序列包含式M1-Za-M2所示的序列或Za所示的序列,其中:
M1、M2各自独立地单个或多个氨基酸多肽区段或者不存在;
Za为Tyr-Leu-X1-X2-X3-X4-Gly-Ala-X5-X6-Pro-X7-Pro-Asp-X8-Leu-Glu-Pro-Thr
X1为Asn或Tyr或Asp或不存在,
X2为Asn或Gln或His或Pro或Ser或不存在,
X3为Gly或Trp或不存在,
X4为Leu或不存在,
X5为Pro或Ser,
X6为Ala或Val,
X7为Tyr或Ser或Ala,
X8为Pro或Thr,
进一步地,其中Za选自其中之一:
本发明另一方面提供了一种靶向卵巢多肽的衍生物,所述衍生物为所述多肽氨基酸侧链基团上、氨基端、羧基端进行常规修饰得到的产物,或者为多肽上连接用于多肽或蛋白检测或纯化的标签所得到的产物,或经过同位素标记修饰得到的产物;所述的常规修饰为荧光基团修饰、磷酸化修饰、二硫键的环化修饰、经过生物素标记修饰、光敏剂、叠氮修饰、PEG修饰、甲基化修饰、荧光淬灭基团修饰、蛋白偶联修饰、小分子化合物修饰、氨基化修饰、酰胺化修饰、羟基化修饰、羧基化修饰、羰基化修饰、烷基化修饰、乙酰化修饰、酯化修饰、
糖基化修饰;
进一步地,所述的标签为His6、GST、EGFP、MBP、Nus、HA、IgG、FLAG、c-Myc或ProfinityeXact。
进一步地,所述氨基端、羧基端的修饰选自多肽N端乙酰化修饰和C端的胺化修饰。
进一步地,所述侧链的修饰选自多肽中氨基酸侧链R基上的修饰。
进一步地,所述荧光基团的修饰中所用的荧光染料选自AMCA、FITC、Rhodamine、Cy3、Cy5、Cy5.5、Cy7、AIE、ICG,该修饰可用于荧光检测。
进一步地,所述磷酸化修饰选自p-Ser、p-Thr、p-Tyr中的一种或多种的组合。
进一步地,所述糖基化修饰选自Ser、Asn、Thr、Tyr中的一种或多种的组合。
进一步地,所述硝基化修饰选自Tyr中的一种或多种的组合。
进一步地,所述生物素的标记,所述生物素选自D-生物素、生物素酰肼、光敏生物素和生物素-dUTP。
进一步地,所述光敏剂修饰,可用于制备光敏感制剂。
进一步地,所述叠氮修饰,可用于次级的连接反应。
进一步地,所述PEG修饰,可用于制备药物载体。
进一步地,所述同位素标记中所用的同位素选自13C、14C、14N、15N、2H、3H、18O、32P、32S、34S、35S、36S、35Cl、37Cl、125I、131I中的一种或多种的组合。
本发明再一个方面提供了一种多聚核苷酸,其编码上述多肽或其衍生物。
本发明再一个方面提供了一种载体,其包含了上述多聚核苷酸。
本发明再一个方面提供了一种宿主细胞,其转染了上述载体。
本发明再一个方面提供了本发明所述多肽或其衍生物在制备靶向卵巢的检测试剂、靶向卵巢的药物或靶向卵巢的工具中的用途。
进一步地,所述工具选自载体或制剂,例如通过偶联本发明所述多肽或其衍生物实现靶向效果的载体、制剂或试剂盒,制剂如脂质体、纳米粒等。
本发明另一个方面提供了上述多肽或其衍生物在制备能够靶向卵巢的药物或检测试剂中的用途。
进一步地,所述药物和检测试剂包括上述多肽及以其为骨架的衍生物,作为抗原制备的检测产品如检测试剂、试剂盒、试纸条。
本发明另一个方面提供了一种靶向卵巢的载体,上述多肽及以其为骨架的衍生物可作为靶向治疗卵巢相关生殖疾病的工具。
进一步地,所述载体选自脂质体、纳米粒、外泌体、生物可降解高分子物质、抗体-配体。
进一步地,所述载体中含有可治疗卵巢相关生殖疾病的成分。
所述的多肽及以其为骨架的衍生物可通过一般化学实验室条件自主合成,可通过基因表达出来的含有上述多肽的重组蛋白,也可以由商业化试剂公司工业合成,采用固相法合成多肽,在树脂上不同氨基酸,经过缩合反应定向合成氨基酸链。多肽的衍生物,是在氨基酸完成连接后,再标记上修饰基团。
本发明再一方面提供了一种靶向卵巢的药物,所述药物为上述多肽及以其为骨架的衍生物与活性成分的偶联物。所述的多肽及以其为骨架的衍生物可通过靶向卵巢治疗因生殖细胞异常和激素紊乱导致的生殖、代谢及衰老相关疾病。
本发明再一方面提供了一种靶向卵巢的多肽探针,所述多肽探针为上述多肽或以其为骨架的衍生物,与荧光染料通过有机化学反应偶联制得。
进一步地,所述荧光染料选择具有近红外区荧光基团的染料,优选地,所述具有近红外区荧光基团的染料选自AIE、Cy5、Cy7、ICG。
本发明再一方面提供了所述多肽在制备治疗的卵巢相关生殖疾病的药物或试剂中的用途。
进一步地,卵巢相关生殖疾病选自生殖细胞异常或激素紊乱导致的卵巢相关生殖疾病。
进一步地,所述激素紊乱为雄性激素超过正常水平。
进一步地,所述生殖细胞异常导致的卵巢相关生殖疾病为由于卵母细胞老化,和或卵母细胞透明袋硬化导致的卵巢相关生殖疾病,优选地为所述的生殖细胞异常导致的卵巢相关生殖疾病选自卵母细胞数量异常、卵母细胞质量下降和卵子老化。
进一步地,所述的因激素紊乱导致的卵巢相关生殖疾病包括月经周期紊乱、多囊卵巢综合征、雄化表型、毛发脱落、代谢异常、卵巢功能不全、卵巢早衰、和排卵障碍。
进一步地,所述的因激素紊乱导致的代谢异常选自糖代谢异常、脂代谢异常,优选为高血糖、胰岛素抵抗、肥胖、脂肪肝、骨质疏松。
进一步地,雄化表型选自痤疮、多毛、脱发。
进一步地,所述的因激素紊乱导致的衰老及相关症状包括围绝经期、更年期综合征、衰老、痤疮、长痘、多毛、脱发。
本发明再一方面提供了所述多肽在制备防治卵母细胞在体内或体外老化的药物或试剂中的用途。
本发明再一方面提供了一种辅助生殖用品,其包括本发明所述的多肽或其衍生物。
进一步地,所述辅助生殖用品为辅助生殖过程中卵母细胞和或受精卵的保护液。
本发明再一方面提供了上述辅助生殖用品在制备辅助生殖过程中用于保存卵母细胞和或受精卵的保护液中的用途。
本发明的有益效果:
(1)本发明提供的候选多肽及以其为骨架的衍生物,可以在体内外特异性靶向卵巢。
(2)本发明提供的候选多肽及以其为骨架的衍生物,可作为修饰多肽以增强药物或药物载体对卵巢的靶向性和干预及治疗效果。
(3)本发明提供的候选多肽及以其为骨架的衍生物,其易于合成修饰,成本低廉,因此应用前景广泛。
(4)本发明提供的候选多肽及以其为骨架的衍生物,为其实际应用提供了广泛的诊疗化合物或药物的制备策略。
(5)本发明提供的候选多肽及以其为骨架的衍生物,适用于靶向治疗卵巢相关生殖疾病的应用,也适用于作为卵巢疾病造模实验动物中的研究和观察工具。
图1为连接ICG后的多肽在体内靶向卵巢的效果图。图中显示了PBS组、ICG组和ICG连接多肽组三组小鼠卵巢和子宫的荧光信号分布和结果。
图2为连接AIE后的多肽在体内靶向卵巢的效果图。图中显示了PBS组、AIE空颗粒组、AIE连接多肽组和AIE连接其他多肽组四组小鼠卵巢的荧光信号分布和结果。
图3为连接AIE后的多肽在体内靶向卵巢的定位图。图中显示了PBS组、AIE空颗粒组、AIE连接多肽组和AIE连接其他多肽组四组小鼠卵巢的荧光信号定位。其中,箭头指示膜细胞,星号指示间质细胞。
图4为靶向卵巢多肽在高雄激素-双氢睾酮(DHT)诱导的小鼠模型中,对高雄激素导致的卵泡发育异常、动情周期紊乱、小鼠雄化表型和血糖异常的缓解作用。图4A是对照组、DHT组和DHT+靶向卵巢多肽组三组小鼠卵巢形态学观察。图4B是三组小鼠十天中的动情期天数。图4C是造模60天后,小鼠肛殖区距离(雄性肛殖区距离较大)。图4D是造模60天后,小鼠口服葡萄糖后血糖变化。
图5为靶向卵巢多肽在高雄激素-双氢睾酮(DHT)诱导的小鼠模型中,对高雄激素导致的体重变化的缓解作用。图5A是体重变化,图5B是体重增长量的变化。图5C是性腺脂肪的重量变化,图5D是除脂肪外的瘦体重变化。
图6为靶向卵巢多肽在高雄激素-双氢睾酮(DHT)诱导的小鼠模型中,对高雄激素导致的小鼠颈背部毛发脱落的影响。图6A是对照组、DHT组和DHT+靶向卵巢多肽组三组小鼠颈背部毛发脱落现象的观察。图6B是三组小鼠颈背部皮肤雄激素受体AR表达的免疫荧光图。
图7为靶向多肽在卵母细胞排卵后老化模型中,对老化导致的卵母细胞碎裂与退化、透明带硬化与体外受精情况的改善作用。图7A是对照组、老化组和靶向多肽组老化12小时后退化与碎裂率。图7B是对照组、老化组和靶向多肽组老化12小时透明带硬化水平。图7C是对照组、老化组和靶向多肽组体外老化12小时后体外受精率。
图8为OCN15KO小鼠在超排模型中与WT小鼠相比的血清雌孕激素水平变化。图8的左图为超排过程中雌激素水平变化。图8的右图是超排过程中孕激素水平变化。
图9为靶向卵巢多肽在高雄激素-双氢睾酮(DHT)诱导的小鼠模型中,对高雄激素导致的小鼠肝脏脂滴积累的影响。
图10为不同物种来源的代谢素的序列对比图。
为了更好地理解本发明的内容,下面结合具体实施案例,对本发明内容作进一步说明,但本发明的保护内容不局限以下实施例。
除非另外特别指出,否则本文使用的所有技术和科学术语具有与本发明所属领域的普通技术人员通常理解的含义相同的含义。此外,与本文描述的方法或材料类似或等同的任何方法或材料可用于实施本发明。出于本发明的目的,定义了以下术语。
术语“受试者”、“个体”和“患者”在本文中可互换使用以指脊椎动物,优选哺乳动物。哺乳动物包括但不限于人类、小鼠、大鼠、黑猩猩、猕猴、牛、绵羊、野猪、鼹鼠、狗。还涵盖了在体内获得或在体外培养的生物实体的组织、细胞及其后代。
如本文所用,术语“施用”包括经口施用、局部接触、作为栓剂施用、静脉内、腹膜内、肌内、病灶内、鞘内、鼻内或皮下施用给受试者。施用是通过任何途径,包括肠胃外和经粘膜(如,经颊、舌下、经腭、经牙龈、经鼻、经阴道、经直肠或经皮)。肠胃外施用包括如,静脉内、肌内、动脉内、皮内、皮下、腹膜内、心室内和颅内。其他的递送模式包括但不限于使用脂质体制剂、静脉内输注、经皮贴剂等。
术语“治疗”是指用于获得包括但不限于治疗性益处在内的有益或期望结果的方法。治疗性益处意指治疗中的一种或多种疾病、病况或症状的任何治疗相关的改善,或对治疗中的一种或多种疾病、病况或症状的任何治疗相关的作用。“预防”是指用于获得包括但不限于预
防性益处在内的有益或期望结果的方法。对于预防性益处,组合物可以施用给具有发展特定疾病、病况或症状风险的受试者,或施用给报告疾病的一种或多种生理症状的受试者,即使所述疾病、病况或症状可能尚未表现出来。
术语“治疗有效量”是指肽或组合物的足以实现有益或所需结果的量。治疗有效量可以取决于以下一个或多个而变化:被治疗的受试者和疾病状况、受试者的体重和年龄、疾病状况的严重程度、施用方式等,其可以由本领域普通技术人员容易地确定。具体的量可以取决于以下一个或多个而变化:所选择的特定药剂、靶细胞类型、受试者体内靶细胞的位置、要遵循的给药方案、是否与其他化合物联合施用、施用的时间以及携带它的实体递送系统。
实施例1:多肽序列的制备
采用人工合成的方法合成多肽序列,
所述多肽序列如下所述:
采用常规的固相合成或液相合成的方法合成上述多肽。其中利用固相合成多肽法为从C端的氨基酸向N端反应,经过树脂活化、氨基酸链接、洗脱保护、检测等步骤,逐个完成氨基酸链接,然后用过量乙醚沉淀离心,对粗肽经过HPLC纯化后,质谱分析,在液氮中速冷冻干备用。
在本发明中,所述的肽均为分离的肽。
其中SEQ ID NO.1为小鼠代谢素,SEQ ID NO.2为大鼠代谢素,SEQ ID NO.3为人源代谢素,SEQ ID NO.4为黑猩猩代谢素,SEQ ID NO.5为猕猴代谢素,SEQ ID NO.6为牛代谢素,SEQ ID NO.7为绵羊代谢素,SEQ ID NO.8为野猪代谢素,SEQ ID NO.9为鼹鼠代谢素,SEQ ID NO.10为狗代谢素(如图10所示)。
实施例2:多肽探针的制备
本实施例提供一种多肽探针,采用实施例1制备的多肽,将其与ICG/AIE通过有机化学反应结合。
具体方法为委托合成或自行合成,本实施例中,通过点击化学方法将多肽与ICG或AIE连接完成探针制备,其中的连接子为通用的DBCO。具有活化功能基团的ICG/AIE,活化功能基团包含氨基NH2、羧基COOH、活化脂NHS、马来酰亚胺MAL、巯基SH、叠氮N3、炔烃ALK,然后与实施例1制备的多肽偶联,获得多肽ICG/AIE探针。
实施例3:多肽ICG探针靶向小鼠卵巢能力检测
成年小鼠尾静脉注射实施例2制备的多肽ICG探针(含SEQ ID NO.1)溶液、ICG溶液(2mM)以及PBS,每只小鼠注射100μL,24小时后,在小动物成像仪下检测。
实验结果见图1,通过对比3组结果可知,在卵巢部位多肽ICG探针组(OCN15-ICG,含SEQ ID NO.1的ICG探针)有特异信号,而其他两组(ICG溶液组和PBS组)均未显示在卵巢部位有荧光信号。
以上实验结果可知,本发明的多肽对卵巢有特异性响应,能够实现在体内靶向卵巢的作用。
实施例4:多肽AIE探针靶向小鼠卵巢能力检测
成年小鼠尾静脉注射实施例2制备的多肽AIE探针(含SEQ ID NO.1)、乱序多肽AIE探针、AIE空颗粒以及PBS,每只小鼠注射100μL,24小时后,在小动物成像仪下检测。
实验结果见图2,通过对比4组结果可知,在卵巢部位多肽AIE探针组(OCN15,含SEQ ID NO.1的AIE探针)有特异信号,乱序多肽AIE探针组(OCN22)和PBS组均未显示在卵巢部位有荧光信号,AIE空颗粒组(NP)有微弱的信号。
以上实验结果可知,本发明的多肽对卵巢有特异性响应,能够实现在体内靶向卵巢的作用。
通过对比实施例3和4可知,本发明多肽对卵巢有特异性响应,对其进行的修饰并不会影响其靶向作用,这预示着本发明多肽能够用于制备向卵巢靶向的药物、检测试剂或制剂等。
实施例5:多肽靶向小鼠卵巢的定位
为了进一步确定多肽靶向卵巢的定位,实施例4中的四组小鼠卵巢通过4%PFA固定后,冰冻切片(10μm),染细胞核(DAPI),在激光共聚焦显微镜下观察荧光定位。
实验结果见图3,其中,箭头指示膜细胞,星号指示间质细胞。
通过对比4组结果可知,多肽AIE探针组(OCN15-AIE)主要靶向小鼠卵巢间质细胞和膜细胞。
综上,本发明提供的介导多肽可用于卵巢靶向的目的,尤其是靶向卵巢中的卵巢间质细胞和膜细胞。对于多肽上的修饰并不会影响多肽的作用。
通过实施例5结果可以证明OCN15靶向卵巢间质细胞和膜细胞,可以结合下述实施例结果可以预期其应用于与这两种细胞异常相关的卵巢疾病,如高雄激素引起的膜细胞增厚和间质细胞增生、因膜细胞增厚和间质增生导致的排卵障碍、因排卵障碍导致的卵巢功能不全、月经周期紊乱和多囊卵巢综合征等。
实施例6:靶向卵巢多肽对高雄激素诱导的小鼠生殖和代谢症状的作用
野生型雌鼠在出生后的第25天(D25),随机分为:对照组(CTRL),DHT组,DHT+OCN15(OCT15即SEQ ID NO.1所示多肽)组,每组至少8只小鼠。对照组皮下埋植空泵,每天灌胃200μL生理盐水;DHT组皮下埋植含5mg二氢睾酮(DHT)粉末的缓释泵,每天灌胃200μL生理盐水;DHT+OCN15组皮下埋植含5mg DHT粉末的缓释泵,每天灌胃200μL含多肽的生理盐水(500μg/Kg),造模时间为60天。
实验结果见图4,通过对比3组小鼠可见,高雄激素会诱导小鼠卵巢出现多囊样改变和动情期减少。正常小鼠动情期天数为4天以上,而高雄组小鼠动情天数在2天作用,而同时使用OCN15能够在一定程度上缓解这些现象,动情天数接近3天,而多囊样改变也有所降低(图4A,B)。高雄激素会导致雌性小鼠出现雄化的现象,即肛殖区距离增加,而OCN15可以显著改善这一症状(图4C),可以将肛殖区距离恢复至与正常小鼠接近的程度,其中对照组与DHT组之间,以及DHT组与DHT+OCN15组之间显示了显著性差异。
血糖实验结果见图4D,血糖的峰值应该是在口服葡萄糖15分钟以后,30分钟的时候,对照组已下降,DHT组还是很高,与对照组的差异达3颗星,而OCN15处理后,与单独DHT组相比有显著下降,与对照组没有差异,说明OCN15让DHT诱导的在30min时的血糖异常趋于正常。
体重变化结果见图5,图5的A和B说明OCN15能够在一定程度上缓解DHT导致的小鼠体重和体重增加量的升高,主要减轻了小鼠性腺脂肪的重量(图5C)。OCN15联合DHT处理后的小鼠瘦体重是增加的,瘦体重主要是骨骼的重量,说明OCN15能够缓解因激素紊乱导致骨质疏松。
通过这个实施例结果可以证明OCN15可以应用于高雄激素导致的卵巢相关生殖疾病包括月经周期紊乱、多囊卵巢综合征和雄化表型,以及因激素紊乱导致的代谢异常如糖代谢异常、肥胖和骨质疏松。
此外,观察造模60天时小鼠毛发情况以及小鼠表皮层雄激素受体的表达情况。实验结果
见图6,通过对比3组小鼠可见,高雄激素会导致小鼠颈背部皮肤的毛发出现脱落现象,而OCN15能够显著改善这一现象(图6A)。这可能是由于高雄激素导致雌性小鼠颈背部皮肤中表皮层雄激素受体AR表达的显著增加,进而导致毛发脱落,而OCN15能够显著降低DHT导致的雌性小鼠颈背部皮肤中表皮层雄激素受体AR表达的显著增加(图6B),这一结果也与小鼠颈背毛发脱落情况一致。
通过这个实施例结果可以证明OCN15可以应用于高雄激素导致的相关症状如女性脱发。
实施例7:靶向卵巢多肽对卵母细胞排卵后老化的作用
野生型雌性小鼠腹腔注射5IU PMSG(Pregnant Mare Serum Gonadotropin),48小时后腹腔注射5IU hCG(Human Chorionic Gonadotropin)进行超数排卵。14小时后收集卵母细胞,使用透明质酸酶去除卵丘细胞,随机分为Aging组与Aging+OCN15组并于M16培养液中培养老化。对照组(Control)老化时间0小时,Aging组老化时间12小时,Aging+OCN15组在含多肽的M16(1μg/mL)中老化12小时。
实验结果见图7,通过三组对比,OCN15可以改善卵母细胞体外老化所导致的碎裂与退化(图7A)。OCN15可以改善卵母细胞体外老化所导致的透明带硬化(图7B)。OCN15可以改善卵母细胞体外老化所导致的受精率低下,甚至与老化0小时受精率接近,没有显著性差异。
通过这个实施例结果可以证明OCN15可以应用于卵母细胞质量的保护,延缓其老化过程,改善因老化导致的受精率低下。可以应用于辅助生殖领域,提高卵母细胞以及受精卵的保存时间和保存质量。同时,可以预期由于其具有改善卵母细胞体外老化的作用,因此,可以用于卵母细胞由于老化导致的异常而诱发的疾病,例如卵母细胞数量异常、卵母细胞质量下降和卵子老化。
综上,本发明提供的介导多肽可用于增加卵母细胞受精窗口时间激素紊乱导致的卵巢相关生殖疾病、代谢异常和脱发等症状的治疗。
实施例8:靶向卵巢多肽敲除对小鼠超数排卵过程中血清激素水平的影响
野生型雌性小鼠和OCN15KO小鼠腹腔注射5IU PMSG,48小时后腹腔注射5IU hCG进行超数排卵。在注射前、PMSG24h、PMSG48h、hCG12h分别处死小鼠,取血清检测性激素水平。
实验结果见图8,通过与WT小鼠的比较,可以发现超排过程中雌激素水平没有变化(图8的左图),而孕激素水平在PMSG48h时,OCN15KO小鼠与WT小鼠相比显著升高(图8的右图)。PMSG48h这个时间点,是大部分卵泡被刺激发育到优势卵泡待排卵的阶段,而此
阶段的孕激素主要是由大卵泡的颗粒细胞分泌,说明OCN15KO小鼠在这一阶段与WT相比有更多的卵泡响应了PMSG的刺激,发育到待排卵阶段,因此OCN15KO后可能导致小鼠卵泡过度激活,可能导致卵巢早衰,由此可以证明本发明的靶向卵巢多肽能够用于治疗卵巢早衰。
实施例9:靶向卵巢多肽对高雄激素诱导的小鼠肝脏脂滴积累的作用
高雄激素DHT诱导小鼠PCOS模型的方法见实施例6。造模结束后,取小鼠肝脏,4%多聚甲醛(PFA)固定过夜,酒精梯度脱水,石蜡包埋,切片(5um),HE染色做形态学观察。
实验结果见图9,通过与WT小鼠的肝脏形态比较,可以发现DHT模型小鼠肝脏细胞体积变大,提示胞质中更多的脂滴积累,而在DHT+OCN15处理组,这一现象被缓解。
由此证明本发明靶向卵巢多肽能够缓解高雄激素诱导的小鼠肝脏脂滴积累,用于治疗脂肪肝。
以上所述仅为本发明的具体实施方式,不是全部的实施方式,本领域普通技术人员通过阅读本发明说明书而对本发明技术方案采取的任何等效的变换,均为本发明的权利要求所涵盖。
Claims (12)
- 一种靶向卵巢的多肽,所述多肽序列包含式M1-Za-M2所示的序列或Za所示的序列,其中:M1、M2各自独立地单个或多个氨基酸多肽区段或者不存在;Za为Tyr-Leu-X1-X2-X3-X4-Gly-Ala-X5-X6-Pro-X7-Pro-Asp-X8-Leu-Glu-Pro-ThrX1为Asn或Tyr或Asp或不存在,X2为Asn或Gln或His或Pro或Ser或不存在,X3为Gly或Trp或不存在,X4为Leu或不存在,X5为Pro或Ser,X6为Ala或Val,X7为Tyr或Ser或Ala,X8为Pro或Thr,优选地,其中Za选自其中之一:YLGASVPSPDPLEPT SEQ ID No.1;YLNNGLGAPAPYPDPLEPT SEQ ID No.2;YLYQWLGAPVPYPDPLEPT SEQ ID No.3;YLYQWLGAPVPYPDTLEPT SEQ ID No.4YLYQWLGAPAPYPDPLEPT SEQ ID No.5YLDHWLGAPAPYPDPLEPT SEQ ID No.6YLDPGLGAPAPYPDPLEPT SEQ ID No.7YLDHGLGAPAPYPDPLEPT SEQ ID No.8YLDQGLGAPAPAPDPLEPT SEQ ID No.9YLDSGLGAPVPYPDPLEPT SEQ ID No.10。
- 一种靶向卵巢的多肽的衍生物,其特征在于,所述衍生物为权利要求1所述多肽氨基酸侧链基团上、氨基端、羧基端进行常规修饰得到的产物,或者为权利要求1多肽上连接用于多肽或蛋白检测或纯化的标签所得到的产物,或经过同位素标记修饰得到的产物;所述的常规修饰为荧光基团修饰、磷酸化修饰、二硫键的环化修饰、经过生物素标记修饰、光敏剂、叠氮修饰、PEG修饰、甲基化修饰、荧光淬灭基团修饰、蛋白偶联修饰、小分子化合物修饰、氨基化修饰、酰胺化修饰、羟基化修饰、羧基化修饰、羰基化修饰、烷基化修饰、乙酰化修饰、酯化修饰、糖基化修饰;优选地,所述的标签为His6、GST、EGFP、MBP、Nus、HA、IgG、FLAG、c-Myc或ProfinityeXact;优选地,所述氨基端、羧基端的修饰选自多肽N端乙酰化修饰和C端的胺化修饰;优选地,所述侧链的修饰选自多肽中氨基酸侧链R基上的修饰;优选地,所述荧光基团的修饰中所用的荧光染料选自AMCA、FITC、Rhodamine、Cy3、Cy5、Cy5.5、Cy7、AIE、ICG,该修饰可用于荧光检测;优选地,所述磷酸化修饰选自p-Ser、p-Thr、p-Tyr中的一种或多种的组合;优选地,所述糖基化修饰选自Ser、Asn、Thr、Tyr中的一种或多种的组合;优选地,所述硝基化修饰选自Tyr中的一种或多种的组合;优选地,所述生物素的标记,所述生物素选自D-生物素、生物素酰肼、光敏生物素和生物素-dUTP;优选地,所述同位素标记中所用的同位素选自13C、14C、14N、15N、2H、3H、18O、32P、32S、34S、35S、36S、35Cl、37Cl、125 I、131I中的一种或多种的组合。
- 一种多聚核苷酸,其编码权利要求1所述多肽或权利要求2所述衍生物。
- 一种载体,其包含了权利要求3所述多聚核苷酸。
- 一种宿主细胞,其转染了权利要求4所述载体。
- 权利要求1所述多肽或权利要求2所述衍生物在制备靶向卵巢的检测试剂、靶向卵巢的药物或靶向卵巢的工具中的用途;优选地,所述工具选自载体或制剂,更优选为通过偶联本发明所述多肽或其衍生物实现靶向效果的载体、制剂或试剂盒,更优选制剂为脂质体、纳米粒。
- 权利要求1所述多肽或权利要求2所述衍生物在制备能够靶向卵巢的药物或检测试剂中的用途;优选地,所述药物和检测试剂包括权利要求1所述多肽和或权利要求2所述衍生物,作为抗原制备的检测产品,更优选地,所述检测产品为检测试剂、试剂盒、试纸条。
- 一种靶向卵巢的载体,其以权利要求1所述多肽和或权利要求2所述衍生物作为靶向 剂;优选地,所述载体选自脂质体、纳米粒、外泌体、生物可降解高分子物质、抗体-配体;优选地,所述载体中含有可治疗卵巢相关生殖疾病的成分。
- 一种靶向卵巢的药物,所述药物为权利要求1所述多肽和或权利要求2所述衍生物与活性成分的偶联物。
- 一种靶向卵巢的多肽探针,所述多肽探针为权利要求1所述多肽和或权利要求2所述衍生物,与荧光染料通过有机化学反应偶联制得;优选地,所述荧光染料选择具有近红外区荧光基团的染料,更优选地,所述具有近红外区荧光基团的染料选自AIE、Cy5、Cy7、ICG。
- 权利要求1所述多肽和或权利要求2所述衍生物在制备治疗的卵巢相关生殖疾病或缓解卵巢相关生殖疾病的症状的药物或试剂中的用途;所述卵巢相关生殖疾病选自生殖细胞异常导致的卵巢相关生殖疾病、激素紊乱导致的卵巢相关生殖疾病、因激素紊乱导致的衰老及相关症状;或者在制备防止卵母细胞在体内或体外老化的药物或试剂中的用途;优选地,所述激素紊乱为雄性激素超过正常水平;优选地,所述生殖细胞异常导致的卵巢相关生殖疾病为由于卵母细胞老化,和或卵母细胞透明袋硬化导致的卵巢相关生殖疾病,更优选地,所述的生殖细胞异常导致的卵巢相关生殖疾病选自卵母细胞数量异常、卵母细胞质量下降和卵子老化;优选地,所述的激素紊乱导致的卵巢相关生殖疾病选自月经周期紊乱、多囊卵巢综合征、雄化表型、毛发脱落、代谢异常、卵巢功能不全、卵巢早衰和排卵障碍;更优选地,雄化表型选自痤疮、多毛、脱发;优选地,所述的因激素紊乱导致的代谢异常选自糖代谢异常、脂代谢异常,更优选为高血糖、胰岛素抵抗、肥胖、脂肪肝、骨质疏松;优选地,所述的因激素紊乱导致的衰老及相关症状包括围绝经期、更年期综合征。
- 一种辅助生殖用品,其包括权利要求1所述多肽和或权利要求2所述衍生物;优选地,所述辅助生殖用品为辅助生殖过程中卵母细胞和或受精卵的保护液。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210479815.7A CN117050142A (zh) | 2022-05-05 | 2022-05-05 | 一种靶向卵巢的多肽及其衍生物和应用 |
CN202210479815.7 | 2022-05-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2023213141A1 true WO2023213141A1 (zh) | 2023-11-09 |
Family
ID=88646213
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2023/081313 WO2023213141A1 (zh) | 2022-05-05 | 2023-03-14 | 一种靶向卵巢的多肽及其衍生物和应用 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN117050142A (zh) |
WO (1) | WO2023213141A1 (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106892975A (zh) * | 2017-03-16 | 2017-06-27 | 深圳先进技术研究院 | 调节糖代谢的多肽及其用途 |
CN107011427A (zh) * | 2017-03-16 | 2017-08-04 | 深圳先进技术研究院 | 调节能量代谢的多肽及其用途 |
CN109957007A (zh) * | 2017-12-22 | 2019-07-02 | 深圳先进技术研究院 | 一种代谢素多肽人工抗原以及抗体和应用 |
CN114288414A (zh) * | 2021-12-07 | 2022-04-08 | 深圳先进技术研究院 | 一种跨血脑屏障的多肽及其衍生物和应用 |
CN114306630A (zh) * | 2021-12-07 | 2022-04-12 | 深圳先进技术研究院 | 一种靶向脑瘤的多肽及其衍生物和应用 |
-
2022
- 2022-05-05 CN CN202210479815.7A patent/CN117050142A/zh active Pending
-
2023
- 2023-03-14 WO PCT/CN2023/081313 patent/WO2023213141A1/zh unknown
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106892975A (zh) * | 2017-03-16 | 2017-06-27 | 深圳先进技术研究院 | 调节糖代谢的多肽及其用途 |
CN107011427A (zh) * | 2017-03-16 | 2017-08-04 | 深圳先进技术研究院 | 调节能量代谢的多肽及其用途 |
CN109957007A (zh) * | 2017-12-22 | 2019-07-02 | 深圳先进技术研究院 | 一种代谢素多肽人工抗原以及抗体和应用 |
CN114288414A (zh) * | 2021-12-07 | 2022-04-08 | 深圳先进技术研究院 | 一种跨血脑屏障的多肽及其衍生物和应用 |
CN114306630A (zh) * | 2021-12-07 | 2022-04-12 | 深圳先进技术研究院 | 一种靶向脑瘤的多肽及其衍生物和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN117050142A (zh) | 2023-11-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101669140B1 (ko) | 항비만 및 항당뇨 효능을 갖는 펩타이드 및 이의 용도 | |
US10561703B2 (en) | Method of modulating sex hormone levels using a sex hormone secretion modulator | |
JP5355561B2 (ja) | 生物活性ペプチドおよびその使用方法 | |
US20080207543A1 (en) | Modified pituitary gland development in offspring from expectant mother animals treated with growth hormone releasing hormone therapy | |
BRPI0911650B1 (pt) | Peptídeo tendo uma atividade de um fator de crescimento (gf) e derivado de gf e composição farmacêutica para aperfeiçoamento de uma condição de pele e para o tratamento de um ferimento | |
CN101400364A (zh) | 用基于神经丝状蛋白的肽预防或降低癌症风险或发病率的方法 | |
AU2005249373A1 (en) | Use of GPCR54 ligands for the treatment of infertility | |
KR102623470B1 (ko) | Bph 증상의 악화 또는 진행을 개선하거나 예방하는 방법 | |
KR101887577B1 (ko) | 항비만 및 항당뇨 효능을 갖는 펩타이드 및 이의 용도 | |
WO2023213141A1 (zh) | 一种靶向卵巢的多肽及其衍生物和应用 | |
US8980843B2 (en) | Leptin agonist and methods of use | |
Smolinska et al. | Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs | |
Kim et al. | Effects of recombinant gonadotropin hormones on the expression of vitellogenin, gonadotropin subunits and gonadotropin receptors in cinnamon clownfish, Amphiprion melanopus | |
JPH05503699A (ja) | 雌の生殖能の増大法 | |
Kunii et al. | The immunohistochemical expression profile of osteopontin in normal human tissues using two site-specific antibodies reveals a wide distribution of positive cells and extensive expression in the central and peripheral nervous systems | |
WO2023184332A1 (zh) | 一种靶向卵巢多肽及其应用 | |
CN110694056A (zh) | 一种fsh抗原及其制备方法以及含有该抗原的fsh疫苗 | |
Banks et al. | The prolactin receptor: A cross‐species comparison of gene structure, transcriptional regulation, tissue‐specificity, and genetic variation | |
CN114656524B (zh) | 一种靶向卵巢多肽及其应用 | |
CN109125307B (zh) | 一种克罗米酚-多肽复合物、药物制剂及其制备方法和应用 | |
CA2575370A1 (en) | Methods for treating premature infants | |
Newton et al. | Modulators of GnRH Secretion and Therapeutic Applications | |
CN113956331A (zh) | 异位子宫内膜识别多肽及其衍生物和应用 | |
WO2024108216A1 (en) | Compositions comprising octadecaneuropeptides (odn) and synthetic derivatives thereof and methods of use for modulation of food intake, obesity, body weight, nausea, and emesis | |
Pirtea et al. | Rooted in pre-assisted reproductive technology times menotropins are still used today: a narrative review of literature |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 23799129 Country of ref document: EP Kind code of ref document: A1 |