Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
  • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
  • C07K5/08—Tripeptides
  • C07K5/0815—Tripeptides with the first amino acid being basic
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
  • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
  • C07D209/20—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals substituted additionally by nitrogen atoms, e.g. tryptophane
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
  • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
  • C07D209/26—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals with an acyl radical attached to the ring nitrogen atom
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
  • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
  • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
  • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
  • Y02P20/00—Technologies relating to chemical industry
  • Y02P20/50—Improvements relating to the production of bulk chemicals
  • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Definitions

• the invention relates to a novel process for manufacturing a compound of formula (I), or a salt thereof, wherein PG 1 , PG 2 and PG 3 are amino protective groups.
• the process according to the invention is particularly suitable for large-scale manufacturing under GMP conditions.
• the compound of formula (la) is a crucial precursor in the synthesis of the novel antibiotic 1:
• WO20 19206853 discloses a laboratory scale synthesis of the compound of formula (la), which relies on a solid phase synthesis of a particular tripeptide.
• solid-phase synthesis is not suitable for industrial scale manufacturing of the compound of formula (la) due to various issues, such as low yields, long reaction times and epimerization of certain stereocentres.
• the present invention provides a solution phase process for manufacturing compounds of formula (I), which overcomes the problems outlined above.
• the present invention also provides certain intermediates that are useful in the new process.
• the present invention provides a new method for Fmoc deprotection of Fmoc protected amines.
• PG protecting group denotes a group which selectively blocks a reactive site in a multifunctional compound such that a chemical reaction can be carried out selectively at another unprotected reactive site in the meaning conventionally associated with it in synthetic chemistry. Protective groups can be removed at the appropriate point.
• amino protective groups are Boc (tert-butoxycarbonyl), benzyl, 4-methoxybenzyl, benzhydryl, Fmoc (fluorenylmethoxycarbonyl), Cbz (benzyloxycarbonyl), Moz (p- methoxybenzyloxy carbonyl), Troc (2,2,2-trichloroethoxycarbonyl), Teoc (2- (Trimethylsilyl)ethoxycarbonyl), Adoc (adamantoxycarbonyl), formyl, acetyl, and cyclobutoxycarbonyl.
• Further particular amino protective groups are tert-butoxycarbonyl (Boc) and fluorenylmethoxycarbonyl (Fmoc).
• Exemplary carboxylic acid protective groups are allyl and 9-fluorenylmethyl (Fm).
• Exemplary amino and carboxylic acid protective groups and their application in organic synthesis are described, for example, in “Protective Groups in Organic Chemistry” by T. W. Greene and P. G. M. Wutts, 5th Ed., 2014, John Wiley & Sons, N.Y, which is included herein by reference in its entirety.
• salt refers to any kind of salts formed by reacting the compounds disclosed herein with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, in particular hydrochloric acid, and organic acids such as acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p- toluenesulfonic acid, salicylic acid, N-acetylcystein and the like.
• inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, in particular hydrochloric acid
• organic acids such as acetic acid, propionic acid, glycolic acid,
• salts may also be prepared by addition of an inorganic base or an organic base to the free acid.
• Salts derived from an inorganic base include, but are not limited to, the sodium, potassium, lithium, ammonium, calcium, magnesium salts and the like.
• Salts derived from organic bases include, but are not limited to salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, lysine, arginine, N-ethylpiperidine, piperidine, polyimine resins and the like.
• the present invention provides a process for manufacturing a compound of formula (I), or a salt thereof, comprising:
• the present invention provides a manufacturing process as disclosed herein, wherein the mixture used in step (a) is a mixture of HO At and DIC.
• the present invention provides a manufacturing process as disclosed herein, wherein the reagents used in step (a) are a mixture of HOPO and DIC.
• the present invention provides a manufacturing process as disclosed herein, wherein step (a) is performed in a solvent selected from:
• the present invention provides a manufacturing process as disclosed herein, wherein, in step (a), the reagents used are a mixture of HO At and DIC and the solvent is a mixture of tert-butyl methyl ether, //-heptane and dimethylacetamide.
• the present invention provides a manufacturing process as disclosed herein, wherein, in step (a), the reagents used are a mixture of HOPO and DIC; and the solvent is selected from:
• the present invention provides a manufacturing process as disclosed herein, wherein, in step (a), the reagents used are a mixture of HOPO and DIC; and the solvent is selected from:
• the present invention provides a manufacturing process as disclosed herein, wherein, in step (a), the reagents used are a mixture of HOPO and DIC; and the solvent is a mixture of isopropylacetate and l,3-dimethyl-2- imidazolidinone.
• the present invention provides a manufacturing process as disclosed herein, wherein, in step (a), the reagents used are a mixture of HOPO and DIC; and the solvent is a mixture of tert-butyl methyl ether and l,3-dimethyl-2- imidazolidinone.
• the present invention provides a manufacturing process as disclosed herein, further comprising:
• the present invention provides a manufacturing process as disclosed herein, further comprising: (bl) reacting said compound of formula (IV), wherein PG 4 is Fmoc, with N- acetylcysteine and tAmNH2 or tBuNH2, to form a compound of formula (V): wherein PG 1 , PG 2 , and PG 3 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc, and PG 5 is a carboxylic acid protective group selected from allyl and 9-fluorenylmethyl; and
• said basic aqueous solution is an aqueous solution of KHCO3 and/or K2CO3.
• said step (b2) consists of
• the present invention provides a manufacturing process as disclosed herein, further comprising:
• PG 1 , PG 2 , PG 3 and PG 6 are amino protective groups, and PG 5 is a carboxylic acid protective group.
• the present invention provides a manufacturing process as disclosed herein, further comprising:
• PG 5 is a carboxylic acid protective group selected from allyl and 9-fluorenylmethyl.
• the present invention provides a manufacturing process as disclosed herein, wherein, in step (c), said reducing agent is NaBH(OAc)3 and said carboxylic acid is acetic acid. In one embodiment, the present invention provides a manufacturing process as disclosed herein, further comprising:
• the present invention provides a manufacturing process as disclosed herein, further comprising:
• said transition metal catalyst used in step (d) is a palladium catalyst.
• said transition metal catalyst used in step (d) is a palladium (0) catalyst.
• said transition metal catalyst used in step (d) is (PPh 3 ) 4 Pd.
• said secondary amine used in step (d) is Et2NH.
• said secondary amine used in step (d) is Et2NH and said transition metal catalyst is (PPhs ⁇ Pd.
• the present invention provides a manufacturing process as disclosed herein, wherein step (d) is performed in acetonitrile and further comprises working up the reaction mixture obtained from step (d) by:
• step (d4) distilling off the secondary amine from step (d);
• the present invention provides a manufacturing process as disclosed herein, further comprising:
• the present invention provides a manufacturing process as disclosed herein, further comprising:
• the present invention provides a process for manufacturing a compound of formula (I), or a salt thereof, comprising:
• step (d4) distilling off the secondary amine from step (d);
• the present invention provides a process for manufacturing a compound of formula (IVb) described herein, or a salt thereof, comprising:
• the present invention provides a process for manufacturing a compound of formula (Vb) described herein, or a salt thereof, comprising:
• the present invention provides a process for manufacturing a compound of formula (Vllb) described herein, or a salt thereof, comprising:
• the present invention provides a process for manufacturing a compound of formula (IX) described herein, or a salt thereof, comprising:
• the present invention provides a process for manufacturing a compound of formula (IX) described herein, or a salt thereof, comprising:
• step (d) reacting said compound of formula (Vllb) with a transition metal catalyst in the presence of a secondary amine, to form said compound of formula (IX); followed by working up the reaction mixture obtained from step (d) by:
• step (d2) adding N-acetylcysteine to said reaction mixture obtained from step (d); (d3) adding Cy2NH to the reaction mixture obtained from step (d2);
• step (d4) distilling off the secondary amine from step (d); and (d5) filtering the reaction mixture obtained from step (d4).
• the present invention provides a process for manufacturing a compound of formula (la), which is:
• the present invention provides a compound of formula (I) described herein, or a salt thereof, when manufactured according to the processes of the invention.
• the present invention provides a process for manufacturing a compound of formula (1), or a salt thereof, comprising any of the processes described herein.
• the present invention provides the use of any of the process described herein in the manufacture of the compound of formula (1) or a salt thereof.
• PG 1 is BOC.
• PG 3 is BOC.
• PG 4 is Fmoc
• PG 5 is allyl
• PG 6 is Fmoc.
• PG 1 is BOC
• PG 2 is BOC
• PG 4 is Fmoc.
• PG 3 is BOC
• PG 5 is allyl.
• PG 1 is BOC
• PG 2 is BOC
• PG 3 is BOC
• PG 4 is Fmoc
• PG 5 is allyl.
• PG 1 is BOC
• PG 2 is BOC
• PG 3 is BOC
• PG 5 is allyl.
• PG 1 is BOC
• PG 2 is BOC
• PG 3 is BOC
• PG 5 is allyl
• PG 6 is Fmoc.
• PG 1 is BOC
• PG 2 is BOC
• PG 3 is BOC.
• the present invention provides a compound of formula (II) or a salt thereof, wherein PG 1 , PG 2 and PG 4 are amino protective groups.
• the present invention provides a compound of formula (II) as described herein, or a salt thereof, wherein PG 1 , PG 2 and PG 4 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc.
• the present invention provides a compound of formula (II) as described herein, or a salt thereof, wherein said compound of formula (II) is a compound of formula (Ila)
• the present invention provides a compound of formula (III) or a salt thereof, wherein PG 3 is an amino protective group and PG 5 is a carboxylic acid protective group.
• the present invention provides a compound of formula (III) as described herein, or a salt thereof, wherein PG 3 is an amino protective group selected from BOC, Adoc, Moz, and Fmoc, and PG 5 is a carboxylic acid protective group selected from allyl and 9-fluorenylmethyl.
• the present invention provides a compound of formula (III) as described herein, or a salt thereof, wherein said compound of formula (III) is a compound of formula (Illa)
• the present invention provides a compound of formula (IV) or a salt thereof, wherein PG 1 , PG 2 , PG 3 and PG 4 are amino protective groups, and PG 5 is a carboxylic acid protective group.
• the present invention provides a compound of formula (IV) as described herein, or a salt thereof, wherein PG 1 , PG 2 , PG 3 and PG 4 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc, and PG 5 is a carboxylic acid protective group selected from allyl and 9-fluorenylmethyl.
• the present invention provides a compound of formula (IV) as described herein, or a salt thereof, wherein said compound of formula (IV) is a compound of formula (IVa)
• the present invention provides a compound of formula (V) or a salt thereof, wherein PG 1 , PG 2 and PG 3 are amino protective groups, and PG 5 is a carboxylic acid protective group.
• the present invention provides a compound of formula (V) as described herein, or a salt thereof, wherein PG 1 , PG 2 and PG 3 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc, and PG 5 is a carboxylic acid protective group selected from allyl and 9-fluorenylmethyl.
• the present invention provides a hemiphosphate salt of the compound of formula (V) as described herein.
• the present invention provides a compound of formula (V) as described herein, or a salt thereof, wherein said compound of formula (V) is a compound of formula (Va)
• the present invention provides a hemiphosphate salt of the compound of formula (Va) as described herein.
• the present invention provides a compound of formula (VI) as described herein, or a salt thereof.
• the present invention provides a compound of formula (VI) as described herein, or a salt thereof, wherein PG 6 is an amino protective group selected from BOC, Adoc, Moz, and Fmoc.
• the present invention provides a compound of formula (VI) as described herein, or a salt thereof, wherein said compound of formula (VI) is a compound of formula (Via)
• the present invention provides a compound of formula (VII) or a salt thereof, wherein PG 1 , PG 2 , PG 3 and PG 6 are amino protective groups, and PG 5 is a carboxylic acid protective group.
• the present invention provides a compound of formula (VII) as described herein, or a salt thereof, wherein PG 1 , PG 2 , PG 3 and PG 6 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc, and PG 5 is a carboxylic acid protective group selected from allyl and 9-fluorenylmethyl.
• the present invention provides a compound of formula (VII) as described herein, or a salt thereof, wherein said compound of formula (VII) is a compound of formula (Vila)
• the present invention provides a compound of formula (VIII) or a salt thereof, PG 1 , PG 2 and PG 3 and PG 6 are amino protective groups.
• the present invention provides a compound of formula (VIII) as described herein, or a salt thereof, wherein PG 1 , PG 2 and PG 3 and PG 6 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc.
• the present invention provides a compound of formula (VIII) as described herein, or a salt thereof, wherein said compound of formula (VIII) is a compound of formula (Villa)
• the present invention provides a compound of formula (IX)
• PG 1 , PG 2 and PG 3 are amino protective groups.
• the present invention provides a compound of formula (IX) as described herein, or a salt thereof, wherein PG 1 , PG 2 and PG 3 are amino protective groups independently selected from BOC, Adoc, Moz, and Fmoc.
• the present invention provides a compound of formula (IX) as described herein, or a salt thereof, wherein said compound of formula (IX) is a compound of formula (IXa) Novel Method for Fmoc Deprotection
• US9334302 discloses a method of removing a Fmoc protective group from an amine, comprising the addition of a mercaptocarboxylic acid to the dibenzofiilvene (DBF) Fmoc deprotection byproduct, followed by the removal of the adduct by a basic aqueous wash:
• DPF dibenzofiilvene
• the present invention therefore provides a new method for Fmoc deprotection, whereby said byproduct is removed by simple filtration, rather than washing with a basic aqueous solution.
• t-amylamine and dicyclohexylamine can form salts with the DBF-N-acetylcysteine adduct and that those salts exhibit a poor solubility in selected organic solvents, like MeCN:
• the Fmoc protective group is removed by reacting a Fmoc protected amine with a base, such as diethylamine, dicyclohexylamine or t-amylamine.
• the dibenzofiilvene byproduct that is formed in this reaction is then captured by forming an adduct with N- acetylcysteine.
• the base that was used for the removal of the Fmoc protective group was a base other than dicyclohexylamine or t-amylamine
• the adduct is subsequently reacted with dicyclohexylamine or t-amylamine. This results in the formation an insoluble salt, which precipitates from the reaction mixture and can conveniently be filtered off.
• the base used to effect the Fmoc deprotection is an amine other than dicyclohexylamine or t-amylamine, e.g., diethylamine, it may also form a salt with the N-acetylcysteine DBF adduct which does not precipitate from the reaction mixture.
• the presence of such a base may therefore hamper the desired removal of the adduct salt by filtration. It may hence be desirable to remove an excess of this amine reagent by distillation, driving the equilibrium towards the adduct dicyclohexylamine or t-amylamine salt, hence maximizing the precipitation of the corresponding dicyclohexylamine or t-amylamine salt.
• the product IXa comprises a carboxylic acid functional group which would preclude a simple basic aqueous wash to remove any DBF- mercaptocarboxylic acid adduct side products.
• the present invention provides a method for removing an Fmoc protective group from a compound comprising an Fmoc protected amine, comprising:
• step (c) provided the base in step (a) was not Cy2NH or t-amylamine, adding a base selected from Cy2NH and t-amylamine to the reaction mixture obtained from step (b);
• step (d) optionally distilling off the base from step (a);
• the present invention provides a method for removing a Fmoc protective group, comprising:
• step (b) provided the base in step (a) was not Cy2NH or t-amylamine, adding a base selected from Cy2NH and t-amylamine to the reaction mixture obtained from step (a);
• step (c) optionally distilling off the base from step (a);
• the method is performed in acetonitrile as a solvent.
• the base used in step (a) is diethylamine.
• the base used in step (c) is Cy2NH.
• the method for removing a Fmoc protective group according to the invention comprises step (d), distilling off the base from step (a).
• the reaction mixture obtained in step (b) is heated to 30 °C to reflux.
• the reaction mixture obtained in step (b) is heated to 30 °C to 70 °C.
• the reaction mixture obtained in step (b) is heated to 40 °C to 60 °C. In a particularly preferred embodiment, the reaction mixture obtained in step (b) is heated to 50 °C.
• step (a) of the method according to the invention it has surprisingly been found that if >10 equivalents of base are used in step (a) of the method according to the invention, the Fmoc deprotection proceeds smoothly at room temperature.
• >10 equivalents of base are used relative to compound comprising an Fmoc protected amine in step (a) of the method according to the invention.
• steps (a)-(c) of the method according to the invention are performed at room temperature.
• >10 equivalents of base are used relative to the compound comprising an Fmoc protected amine in step (a) of the method according to the invention, and steps (a)-(c) of the method according to the invention are performed at room temperature.
• the method for removing a Fmoc protective group according to the invention comprises:
• the method for removing a Fmoc protective group according to the invention comprises:
• the method for removing a Fmoc protective group according to the invention comprises:
• the reagent mixture used in step (a) further comprises seed crystals of a DBF-N-acetylcysteine adduct Cy2NH or t-amylamine salt.
• the basic aqueous wash described in US9334302 may not be applicable if, for example, the desired product contains an acidic functional group, since it would be washed out into the aqueous phase together with the DBF-mercaptocarboxylic acid adduct byproduct.
• the compound comprising an Fmoc protected amine used in the method according to the invention further comprises at least one carboxylic acid moiety, preferably one to three carboxylic acid moieties, most preferably one carboxylic acid moiety.
• Fmoc-Orn(Boc)-OH (50.0 g, 0.11 mol), N-hydroxy succinimide (13.9 g, 0.12 mol) and THF (200 mL) were charged into a reaction vessel.
• the reaction mixture was stirred for further 21 hours.
• the precipitate was filtered off and washed with THF (30 mL) twice. The filtrate was concentrated at 40-45 °C to 125 mL.
• EtOAc 150 mL was added and the mixture was concentrated at 40-45 °C to 165 mL.
• the reaction mixture was further stirred for 5 hours.
• the reaction mixture was added to water (1200 mL) cooled to 5-10 °C over 2 hours and the mixture was further stirred at 5-10 °C for 30 minutes.
• Ethyl acetate (600 mL) was added.
• the pH was adjusted to 2-3 by addition of 15% aqueous HC1 (10-11 g) keeping the temperature at 5-10 °C.
• the phases were separated.
• the organic layer was washed with aqueous NaCl solution 1.6% (600 g) five times.
• Ethyl acetate (600 mL) was added and the mixture was concentrated to 780-820 g. This operation was repeated four times with the addition of ethyl acetate (480 mL).
• the mixture was concentrated to 960- 1000g.
• the suspension was heated to 40 °C.
• ⁇ Heptane (1500 mL) was added over 1 hour.
• the suspension was further stirred for 1 hour, cooled to 25 °C and stirred 3 hours at this temperature.
• the precipitate was filtered off, washed with ⁇ heptane (300 mL) three times and dried at 45 °C to afford Ila as a white solid. Typically, the yield was about 90%.
• the organic layer was washed with water (260 mL).
• the biphasic mixture was polish filtered over celite.
• the phases were separated and the organic phase was concentrated under reduced pressure to about 300 g.
• Ethyl acetate (540 mL) was added and the solution was concentrated under reduced pressure to about 300 g.
• a Karl Fischer titration was performed and water level should ⁇ 0.05% w/w. Typically the yield was around 98%.
• Step 4 N-Me-Trp(Boc)-OAll oxalate (Illa) N-NBS-N-Me-Trp(Boc)-OAll (171 g, 314.6 mmol) and DMF (684 mL) were charged into a reaction vessel and the system was inertized and cooled to -5 °C. Thiophenolate (65.0 g, 491.8 mmol) was added at -5 - 0 °C over 30 minutes. The reaction mixture was stirred at - 5 °C for 4 hours. Zb/V-butyl methyl ether (1.03 L) was added, followed by water (1.28 L) at -5 - 0 °C (typically 30-60 minutes).
• the reaction mixure was then brought to 20 °C and stirred for 2 hours. The layers were separated. The organic layer was washed with water (513 mL) three times and concentrated under reduced pressure at ⁇ 35 °C to 264 g. Acetone (850 mL) was added. The solution was polish filtered. The solution was cooled to 7 °C and a solution of oxalic acid dihydrate (39.2 g, 310.9 mmol) in acetone (342 mL) was added. The mixture was stirred at 7 °C for 2 hours.
• H-N-Me-Trp(Boc)-OAll oxalate (Illa oxalate, 150 g, 334.5 mmol) and tert-butyl methyl ether (1.1 kg) were charged into a reactor and water (1.95 kg) was added followed by aqueous NaOH 28% (119.5 kg) within 5 minutes (slight gas evolution).
• the biphasic system was stirred at room temperature for 60 minutes and the phases were separated.
• the organic phase was washed with water (750 g), evaporated under reduced pressure and dried under high vacuum.
• the residue was dissolved in ⁇ -heptane (513 g) and the solution was completely evaporated.
• the mixture was cooled to 0 - 5 °C and diisopropylcarbodiimide (44.5 g, 352.6 mmol) was added within 10 minutes.
• the addition funnel was rinsed with tert-butyl methyl ether (200 g).
• the reaction mixture was stirred at -2 - 2 °C for 120 minutes.
• Solution A was added over 30 minutes and the addition line was rinsed with //heptane (68 g). Stirring was pursued for 40 minutes.
• the reaction mixture was warmed to room temperature within 180-240 minutes and stirred further for 15 hours.
• the dropping funnel was rinsed with water (200 g).
• the extraction mixture was stirred for 90 minutes, filtered through a glass filter and the reactor was rinsed with //-heptane (298 g) and the filter cake washed with a mixture of tert-butyl methyl ether (340 g) and //-heptane (157 g).
• the biphasic filtrate was stirred for another 10 minutes, and then the phases were separated.
• the organic layer was washed with a solution of NaHCOs (56.5 g) and water (1.25 kg), and three times with a mixture of methanol (871 g) and water (1.1 kg).
• the organic layer was concentrated under reduced pressure to a volume of about 900 mL (solution gets more viscous).
• H-N-Me-Trp(Boc)-OAll oxalate (Illa oxalate, 80 g, 178.4 mmol) and tert-butyl methyl ether (587 g) were charged into a reactor and water (1.04 kg) was added followed by aqueous NaOH 30% (59.5 g) within 5 minutes (slight gas evolution).
• the biphasic system was stirred at room temperature for 60 minutes and the phases were separated.
• the organic phase was washed with water (400 g), evaporated under reduced pressure and dried under high vacuum.
• the residue was dissolved in heptane (275 g) and completely evaporated under reduced pressure to give H-N-MeTrp(Boc)OAll (Illa, 64.6 g, 100%) as a yellow oil.
• a reaction vessel was charged with Fmoc-Orn(Boc)-Lys(Boc)-OH (Ila, 20.5 g, 30.0 mmol), 2-hydroxypyridine-N-oxide (“HOPO”, 1.67 g, 15 mmol) and l,3-dimethyl-2- imidazolidinone (“DMI”, 64.9 g).
• HOPO 2-hydroxypyridine-N-oxide
• DI l,3-dimethyl-2- imidazolidinone
• a solution of H-N-Me- Trp(Boc)-OAll Illa, 11.1 g, 30.9 mmol
• tert-butyl methyl ether 40 g
• the reaction mixture was diluted with tert-butyl methyl ether (91 g).
• H-N-Me-Trp(Boc)-OAll oxalate (Illa oxalate, 150 g, 334.5 mmol) and tert-butyl methyl ether (1.1 kg) were charged into a reactor and water (1.95 kg) was added followed by aqueous NaOH 28% (119.5 g) within 5 minutes (slight gas evolution).
• the biphasic system was stirred at room temperature for 60 minutes and the phases were separated.
• the organic phase was washed with water (750 g), evaporated under reduced pressure and dried under high vacuum.
• the residue was dissolved in heptane (513 g) and completely evaporated under reduced pressure.
• a reaction vessel was charged with Fmoc-Orn(Boc)-Lys(Boc)-OH (Ila, 228.0 g, 334.5 mmol), l-hydroxy-7-azabenzotriazole (22.8 g, 167.1 mmol), dimethylacetamide (155 g), and tert-butyl-methyl ether (1.25 kg).
• To the suspension obtained cooled to 0 °C were added diisopropylcarbodiimide (44.5g, 352.6 mmol) and and tert-butyl methyl ether (200 g). The reaction mixture was stirred at 0 °C for 2 hours. Solution A was added within 30 minutes. The addition funnel was rinsed with nheptane (68 g).
• the reaction mixture was stirred for 40 minutes, then warmed to 22 °C within 3.5 hours, and stirred 16 hours at this temperature.
• a solution of citric acid (103 g, 536 mmol) in water (2.15 kg) was added within 30 minutes.
• the reaction mixture was stirred for 90 minutes, diluted with heptane (297 g) and filtered.
• the precipitate was washed with a mixture of tert-butyl methyl ether (340 g) and heptane (157 g).
• the phases of the filtrate were separated.
• the organic layer was washed with a solution of sodium bicarbonate (56.5 g) in water (1.25 kg) then 3 times with a mixture of methanol (871 g) and water (1.1 kg).
• Table 1 shows a screening of reaction conditions for the coupling of amine Illa with carboxylic acid Ila (see also Examples 4 and 5 above).
• a reaction vessel was charged with a solution of Fmoc-Orn(Boc)-Lys(Boc)-N(Me)- Trp(Boc)-OAll (IVa, 177.2 g, 173.2 mmol) and 1093 mL DMSO at room temperature.
• N- Acetyl-cysteine (36.7 g, 1.3 eq.) was added and the funnel rinsed with DMSO (100 mL). The reaction mixture was stirred until the solids were completely dissolved.
• a solution of tc/7-butylamine (32.9 g, 2.6 eq.) in DMSO (47 mL) was added over 30-60 minutes keeping the temperature at 18-22 °C.
• a solution of phosphoric acid (85%, 8.45 g, 0.43 eq.) in te/7-butyl methyl ether (138.5 ml) was prepared at 5-10 °C and cooled to 0-5 °C.
• the phosphoric acid solution was added to the solution A keeping the temperature at 0-5 °C (ca. 1-1.5 h).
• the suspension was stirred at 0-5 °C for two more hours.
• Cold //-heptane (3050 mL) were slowly added and stirring was pursued at 0-5 °C for 1 hour.
• N-[2-bromo-6-[(3-formyl-2-pyridyl)thio]benzyl]carbamic acid 9H-fluoren-9- ylmethyl ester (142.32 g, 260.9 mmol, 1 eq.) was added at room temperature and the addition funnel was rinsed with toluene (144.52 mL). This afforded a light yellow suspension.
• Acetic acid (34.47 g, 574.05 mmol, 2.2 eq.) was added at room temperature and the funnel was rinsed with toluene (24. 12 mL).
• reaction mixture was added to a solution of sodium bicarbonate (131.52 g, 1565.58 mmol, 6 eq) and water (1.92 L) over 15 minutes. During the addition, gas evolution and foaming was observed.
• the solution was concentrated under reduced pressure to a volume of 100-110 mL and diluted with acetonitrile (218.0 g). Diethylamine (22.8 g, 10.0 eq.) was added and the addition funnel was rinsed with acetonitrile (4.2 g). Palladium tetrakis(triphenylphosphine) (180.4 mg, 0.005 eq.) was added and the funnel was rinsed with acetonitrile (2.1 g). The reaction mixture was stirred at room temperature for 1.5 hour. V-Acetyl-cysteine (6. 1 g, 1.2 eq.) was added. The funnel was rinsed with acetonitrile (4.2 g) and the reaction mixture was stirred at room temperature for 3 hours.
• Example 12 tert-butyl 3-[[( I IS, 14S, 17S)-22-bromo-14-[ 4-(tert-butoxycarbonylamino)butyl] -11-[ 3- (tert-butoxycarbonylamino)propyl]-l 6-methyl-l 2, 15, 18-trioxo-2-thia-4, 10, 13, 16, 19- pentazatricyclo[ 19.4.0.03, 8 pentacosa-1 ( 25), 3, 5, 7,21, 23-hexaen-l 7-yl methyl indole- 1- carboxylate (la)
• the addition funnel was rinsed with dichloromethane (92.8 mL). The reaction mixture was stirred at room temperature for another 30 minutes. A solution of sodium bicarbonate (48.95 g, 0.583 mol, 4.47 eq.) in water (670.0 mL) was added within 30 minutes (gas evolution) und stirring was pursued for 30 to 40 minutes. The phases were separated. The organic layer was washed twice with a solution of sodium bicarbonate (48.95 g, 0.583 mol, 4.47 eq.) in water (670.0 mL), then with water (670.0 mL). The organic phase was concentrated at 25-35 °C/700-600 mbar to a volume of 479 mL. Crystals started to precipitate during the distillation.
• the addition funnel was washed into the reactor with MeCN (15 mL).
• the reaction mixture was stirred at RT for 20h (IPC: mostly deprotected) then 7h at 50°C (complete deprotection and mostly N-Acetyl-cysteine-DBF adduct).
• the reaction mixture was cooled to RT and filtered to remove the majority of the N-Acetyl-cysteine-DBF adduct.
• the filter cake was washed with MeCN (100 mL).
• the filtrate was concentrated under reduced pressure (50°C 300-60 mbar).

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