WO2023143310A1 - 一种纯化阔叶木制浆预水解液中糖分的方法 - Google Patents
一种纯化阔叶木制浆预水解液中糖分的方法 Download PDFInfo
- Publication number
- WO2023143310A1 WO2023143310A1 PCT/CN2023/072917 CN2023072917W WO2023143310A1 WO 2023143310 A1 WO2023143310 A1 WO 2023143310A1 CN 2023072917 W CN2023072917 W CN 2023072917W WO 2023143310 A1 WO2023143310 A1 WO 2023143310A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- prehydrolyzate
- hardwood pulp
- lignin
- hydrolyzate
- sugar
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 48
- 239000011121 hardwood Substances 0.000 title claims abstract description 24
- 238000004537 pulping Methods 0.000 title abstract description 14
- 150000001720 carbohydrates Chemical class 0.000 title abstract 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 39
- 108010059896 Manganese peroxidase Proteins 0.000 claims abstract description 22
- 238000001179 sorption measurement Methods 0.000 claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 10
- 238000000926 separation method Methods 0.000 claims abstract description 10
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 23
- 108090000790 Enzymes Proteins 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 17
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 14
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 14
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 12
- 241000219000 Populus Species 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 10
- 240000007472 Leucaena leucocephala Species 0.000 claims description 7
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims description 7
- 239000011572 manganese Substances 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 229920001221 xylan Polymers 0.000 claims description 6
- 150000004823 xylans Chemical class 0.000 claims description 6
- 244000166124 Eucalyptus globulus Species 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 229910021536 Zeolite Inorganic materials 0.000 claims description 2
- 239000003463 adsorbent Substances 0.000 claims description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 2
- 235000013373 food additive Nutrition 0.000 claims description 2
- 239000002778 food additive Substances 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 239000010457 zeolite Substances 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 238000010907 mechanical stirring Methods 0.000 claims 1
- 239000002808 molecular sieve Substances 0.000 claims 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 claims 1
- 229920005610 lignin Polymers 0.000 abstract description 46
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 3
- 239000000413 hydrolysate Substances 0.000 abstract 2
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 18
- 239000002994 raw material Substances 0.000 description 8
- 239000002023 wood Substances 0.000 description 8
- 108010029541 Laccase Proteins 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000006731 degradation reaction Methods 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 229920002488 Hemicellulose Polymers 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 238000001027 hydrothermal synthesis Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 238000006116 polymerization reaction Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 108010062085 ligninase Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 description 1
- 229920000875 Dissolving pulp Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008394 flocculating agent Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7004—Monosaccharides having only carbon, hydrogen and oxygen atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Definitions
- the invention belongs to the field of biotechnology, and in particular relates to a method for purifying sugar in hardwood pulp prehydrolyzate.
- Prehydrolysis is an important pretreatment method in the process of biomass classification and utilization.
- the pulping raw material is pretreated by hydrothermal method to dissolve hemicellulose and lignin into the hydrolyzate, thereby improving the subsequent pulping efficiency .
- the main components in the hydrolyzate are hemicellulose, lignin, a small amount of glucose, and degradation by-products such as acetic acid, furfural, and 5-hydroxymethylfurfural. Separating the components in the hydrolyzate and converting them into corresponding products can increase the added value of the hydrolyzate and realize the high-value utilization of biomass resources.
- hemicellulose in hardwood pulp prehydrolyzate The main components of hemicellulose in hardwood pulp prehydrolyzate are xylan and xylose, which can be used to prepare high value-added products such as xylooligosaccharides and xylitol. Xylanase and yeast, etc.
- impurities such as lignin have a strong inhibitory effect on the activity of enzymes and microorganisms, reducing the utilization efficiency of sugar.
- lignin separation methods include physical, chemical and biological methods. Due to single means The removal effect of lignin is limited, and the separation of lignin and sugar is generally achieved by combining various means.
- the physical method mainly uses activated carbon, zeolite, macroporous resin, etc. to adsorb and remove lignin, which is a common purification method;
- the chemical method mainly uses cationic flocculants, such as polyacrylamide, calcium hydroxide, calcium oxide and other drugs and lignin in the hydrolyzed solution. Formation of flocculation and removal.
- the loss of sugar is relatively serious.
- the biological method mainly uses biological enzymes (such as laccase, horseradish peroxidase, etc.) to treat the hydrolyzate, polymerize the lignin in it, and make it easy to remove by physical and chemical methods, because of its mild conditions and small sugar loss. , the use of less chemical agents, environmental protection and green advantages have become research hotspots.
- biological enzymes such as laccase, horseradish peroxidase, etc.
- Patent CN201510278306.8 discloses a lignin degradation solution and its preparation method and its method of degrading lignin, which solves the problem that it is difficult to achieve efficient biodegradation of lignin by using a single ligninase such as laccase.
- the lignin degradation solution is composed of laccase and manganese peroxidase.
- the degradation rate of lignin polymers can reach 30-50%, which significantly improves the degradation conversion efficiency and can be used for wood Cellulose biorefining, biopulping or environmental treatment and other fields.
- the invention provides a method for purifying sugar in hardwood pulp prehydrolyzate.
- the pulping prehydrolyzate involved in the present invention refers to the prehydrolyzate obtained by pretreating pulping fiber raw materials by hydrothermal method.
- the pulping fiber raw material can be fast-growing poplar, fast-growing eucalyptus, acacia and other broad-leaved wood raw materials.
- a first aspect of the present invention provides a method for purifying sugar in hardwood pulp prehydrolyzate, comprising:
- the hydrolyzed solution pretreated by the enzyme is treated with an adsorption material, and the solid and liquid are separated to obtain the product.
- the present invention uses biological enzymes as treatment means to develop a treatment method with high efficiency, environmental protection and simple operation, which is of great significance for the high-value utilization of sugar in hydrolyzate.
- the second aspect of the present invention provides the xylose and xylan purified liquid prepared by the above method.
- the third aspect of the present invention provides the application of the above-mentioned xylose and xylan purified liquid in the preparation of health care drugs or food additives.
- the present invention uses the pulping pre-hydrolyzate as raw material, and in the process of high-value utilization of sugar in the hydrolyzate, manganese peroxidase is used to increase its molecular weight so that it can be easily removed.
- activated carbon and other adsorption materials are used to adsorb and remove lignin, and the loss of sugar is small.
- activated carbon and lignin can recover heat through combustion and pyrolysis.
- the method of the present invention is simple, adopts high-efficiency biological enzyme treatment as a means to improve the purification efficiency, has fewer by-products, and adds less chemical agents. It is an environmentally friendly method, so it has good practical Application value and industrialization prospect.
- the treatment effect of manganese peroxidase on the hydrolyzate in the present invention is derived from the polymerization of lignin (realized by the free radical generation and coupling reaction of phenolic hydroxyl groups), changing the properties of lignin so that it is easy to be removed remove. Therefore, as long as the hydrolyzate contains lignin rich in phenolic hydroxyl groups, it can be well polymerized according to the conditions of the present invention, and it is not specific to the prehydrolyzate of hardwood pulp.
- the polymerization and application of manganese peroxidase to lignin have not been reported.
- the present invention proposes a method for promoting lignin separation and improving sugar purification efficiency based on its polymerization of lignin, and its effect is excellent. In the reported laccase and horseradish peroxidase.
- the operation method of the present application is simple, low in cost, universal, and easy for large-scale production.
- the main components of the obtained prehydrolyzate are hemicellulose, lignin and other degradation products.
- the existence of lignin has seriously hindered the high-value utilization of sugar in the hydrolyzate, resulting in the waste of resources and the loss of economic benefits of enterprises. Therefore, before the high-value utilization of sugar in the hydrolyzate, it is necessary to efficiently separate lignin, and at the same time ensure a high yield of sugar.
- the invention improves the removal efficiency of lignin in the sugar purification process of the prehydrolyzed solution, ensures a higher yield of sugar, and provides reference and technical support for the efficient separation of sugar.
- a method for purifying sugar in hardwood pulp prehydrolyzate the specific steps are:
- Adsorption materials such as activated carbon are used to process the hydrolyzed solution through enzyme pretreatment
- manganese peroxidase is used to treat the pre-hydrolyzed liquid, and then adsorption materials such as activated carbon are used to absorb and remove impurities such as lignin in the hydrolyzed liquid to obtain a purified hydrolyzed liquid.
- the method of using manganese peroxidase to treat and then adopting adsorption materials to absorb changes the properties of lignin in the hydrolyzate through enzyme treatment, polymerizes small molecules of lignin to increase its molecular weight, makes lignin easy to remove, and utilizes activated carbon, etc.
- the adsorption material adsorbs the hydrolyzate after enzyme treatment, improves the removal efficiency of lignin, and lowers the total xylose loss, which is a feasible way to achieve component separation and high-value utilization.
- the prehydrolyzate is treated with manganese peroxidase, preferably at a temperature of 20-50°C; for example, 20°C or 30°C.
- the amount of hydrogen peroxide is 1-30mM ⁇ L -1 ; For example, it can be 4.40, 6.88mM ⁇ L -1 .
- the enzyme dosage is 50-800 U ⁇ L -1 , for example, 375, 487.5 U ⁇ L -1 .
- the amount of Mn 2+ used is 0.01-1 mM ⁇ L -1 , such as 0.25, 0.5 mM ⁇ L -1 .
- the reaction time is 0.5-15 hours, such as 4 or 5 hours.
- the pH is 3.6-6.0, such as 4.5.
- the amount of activated carbon added is 0.2-0.6 wt% of the mass of the hydrolyzed solution. In some embodiments, the activated carbon is adsorbed at room temperature, the reaction time is 5-15min, and the magnetic stirrer rotates at 250-350rpm.
- the plant raw material is fast-growing poplar, fast-growing eucalyptus, acacia and other hardwood pulp fiber raw materials.
- the prehydrolyzate rich in xylose and xylan prepared by the method of treating hardwood pulp prehydrolyzate with manganese peroxidase to purify sugar.
- a method for purifying sugar in hardwood pulp prehydrolyzate the steps are as follows:
- the prehydrolyzate is prepared by treating poplar chips with a hydrothermal method, the mass ratio of poplar chips to water is 1:6, and the pretreatment temperature is 170°C.
- the pretreatment time is 60min. After the reaction, the solid and liquid are separated, the liquid is the prehydrolyzed liquid, and the solid is the hydrolyzed wood chips for subsequent pulping).
- the reaction temperature is 30°C
- the pH is 4.5
- the amount of enzyme added is 375U ⁇ L -1
- the amount of hydrogen peroxide is 6.88mM ⁇ L -1
- the amount of Mn 2+ (MnSO 4 ) is 0.25mM ⁇ L -1
- the shaker The rotation speed was 150 rpm, and after 6 hours of reaction, the enzyme was inactivated in a boiling water bath for 5 minutes to terminate the reaction.
- Comparative example 1 did not undergo enzyme treatment, only utilized 0.2wt% activated carbon to treat the hydrolyzate, and measured lignin removal rate and total xylose yield, which are listed in Table 1.
- a method for purifying sugar in hardwood pulp prehydrolyzate the steps are as follows:
- Manganese peroxidase is used to treat the acacia wood pre-hydrolyzate (the pre-hydrolyzate is prepared by treating the acacia wood chips with a hydrothermal method, the mass ratio of the acacia wood chips to water is 1:6, the pretreatment temperature is 170°C, and the pretreatment time 60min, solid-liquid separation after the reaction, the liquid is the pre-hydrolyzed solution, and the solid is the hydrolyzed wood chips for subsequent pulping).
- the reaction temperature is 30°C
- the pH is 4.5
- the amount of enzyme added is 487.5U ⁇ L -1
- the amount of hydrogen peroxide is 4.4mM ⁇ L -1
- the amount of Mn 2+ (MnSO 4 ) is 0.25mM ⁇ L -1
- shake The bed rotation speed was 150 rpm. After 5 hours of reaction, the enzyme was inactivated in a boiling water bath for 5 minutes to terminate the reaction.
- Comparative example 2 did not undergo enzyme treatment, only utilized 0.4wt% activated carbon to treat the hydrolyzate, and measured lignin removal rate and total xylose yield, which are listed in Table 1.
- a method for purifying sugar in hardwood pulp prehydrolyzate the steps are as follows:
- the prehydrolyzate is prepared by treating poplar chips with hydrothermal method, the mass ratio of poplar chips to water is 1:6, and the pretreatment temperature is 170°C , the pretreatment time is 60min, solid-liquid separation after the reaction, the liquid is the pre-hydrolyzed solution, and the solid is the hydrolyzed wood chips for subsequent pulping).
- the reaction temperature was 35°C
- the pH was 4.5
- the amount of enzyme added was 2000U ⁇ L -1
- the rotation speed of the shaker was 150rpm. After 4 hours of reaction, the enzyme was inactivated in a boiling water bath for 5 minutes to terminate the reaction.
- reaction temperature is 30°C
- the pH is 4.5
- the amount of enzyme added is 375U ⁇ L -1 hydrolyzate
- the amount of hydrogen peroxide is 4.4mM ⁇ L -1
- the amount of Mn 2+ is 0.25mM ⁇ L -1
- the reaction time is Under the condition of 5h, 73.15% lignin in the acacia hydrolyzate can be removed by 0.4wt% activated carbon adsorption, which is 54.26% higher than that of the control example 2 (47.42%), but has little effect on the recovery rate of total xylose.
- the manganese peroxidase has a better effect than the laccase under the respective optimal process conditions.
- manganese peroxidase can modify the lignin in the hydrolyzate to make it easy to be removed by activated carbon adsorption and other means, and has no effect on the recovery rate of sugar, which can significantly improve the separation efficiency of lignin and reduce the cost of chemical agents.
- the amount of input is reduced, and the cost is reduced.
- the enzyme is a kind of green and environmentally friendly agent, which can reduce the environmental load and help protect the environment.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Paper (AREA)
Abstract
本发明属于生物技术领域，涉及一种纯化阔叶木制浆预水解液中糖分的方法，具体步骤为：利用锰过氧化物酶处理阔叶木制浆预水解液，进而采用活性炭等吸附材料处理预水解液，分离得到纯化后的预水解液。本发明的方法有效降低了预水解液中糖分的损失，提高了水解液中木质素的去除率，实现了预水解中糖分的纯化，有利于糖分进一步的高效分离与高值化利用。
Description
本发明要求于2022年1月25日提交中国专利局、申请号为202210088371.4、发明名称为“一种纯化阔叶木制浆预水解液中糖分的方法”的中国专利申请的优先权，其全部内容通过引用结合在本发明中。
本发明属于生物技术领域，具体涉及一种纯化阔叶木制浆预水解液中糖分的方法。
公开该背景技术部分的信息仅仅旨在增加对本发明的总体背景的理解，而不必然被视为承认或以任何形式暗示该信息构成已经成为本领域一般技术人员所公知的现有技术。
预水解是生物质分级利用过程中的重要预处理手段，在溶解浆生产过程中，利用水热法预处理制浆原料将半纤维素和木质素溶出到水解液中，从而提升后续制浆效率。水解液中的主要成分为半纤维素、木质素、少量的葡萄糖，以及乙酸、糠醛、5-羟甲基糠醛等降解副产物。对水解液中的组分进行分离并转化为相应产品，可以提升水解液的附加值，实现生物质资源的高值化利用。阔叶木制浆预水解液中半纤维素的主要组分为木聚糖和木糖，可以用于制备低聚木糖、木糖醇等具有高附加值的产品，在此过程中需用到木聚糖酶和酵母菌等，然而木质素等杂质对酶和微生物的活性具有较强的抑制作用，降低糖分的利用效率。为提高水解液中半纤维素的利用效率，需去除水解液中的木质素。
目前，常用的木质素分离方法有物理法、化学法和生物法等。由于单一手段
对木质素的脱除效果有限，一般采用多种手段相结合的方式实现对木质素与糖分的分离。物理法主要使用活性炭、沸石、大孔树脂等吸附去除木质素，是常用的纯化手段；化学法主要采用阳离子絮凝剂，如聚丙烯酰胺、氢氧化钙、氧化钙等药品与水解液中木质素形成絮凝进而去除。然而采用物理法、化学法去除木质素过程中，糖分损失较为严重。生物法主要采用生物酶(如漆酶、辣根过氧化物酶等)处理水解液，聚合其中的木质素，使其易于通过物理、化学法等方式去除，因其具有条件温和、糖损失小、使用化学药剂少、环保绿色等优点成为研究热点。
专利CN201510278306.8公开了木质素降解液及制备方法以及用其降解木质素的方法，解决现有采用漆酶等单一木质素酶难以实现木质素高效生物降解的问题。所述木质素降解液由漆酶和锰过氧化物酶，与单一木质素酶降解反应体系相比，木质素高分子的降解率可达到30～50％，显著提高降解转化效率，可用于木质纤维素生物炼制、生物制浆或环境处理等领域。但对于锰过氧化物酶对木质素的聚合作用及应用未见报道。
发明内容
针对现有制浆预水解液纯化技术中木质素去除率低、糖分损失率高的问题，本发明提供一种纯化阔叶木制浆预水解液中糖分的方法。
本发明所涉及制浆预水解液是指对制浆纤维原料采取水热法预处理得到的预水解液。所述的制浆纤维原料可以为速生杨木、速生桉木、相思木等阔叶木原料。
为实现上述技术目的，本发明采用如下技术方案：
本发明的第一个方面，提供了一种纯化阔叶木制浆预水解液中糖分的方法，包括：
采用锰过氧化物酶处理预水解液，得到酶预处理的水解液；
采用吸附材料对所述酶预处理的水解液进行处理，固液分离，即得。
本发明利用生物酶作为处理手段，开发了一种高效、绿色环保、操作简单的处理方法，对于水解液中糖分的高值化利用具有重要意义。
本发明的第二个方面，提供了上述的方法制备的木糖和木聚糖纯化液。
本发明的第三个方面，提供了上述的木糖和木聚糖纯化液在制备医疗保健类药物或者食品添加剂中的应用。
本发明的有益效果在于：
(1)本发明以制浆预水解液为原料，在对水解液中糖分高值化利用过程中，通过利用锰过氧化物酶提升其分子量，使其易于被脱除。经酶处理后，利用活性炭等吸附材料吸附去除木质素，糖分损失较小，同时活性炭同木质素可以通过燃烧、热解等方式回收热量。与传统多段纯化法相比，本发明方法简单，采用高效的生物酶处理作为提升纯化效率的手段，副产物较少，添加化学药剂较少，是一种环境好友型的方法，因此具有良好的实际应用价值和工业化前景。
(2)本发明中锰过氧化物酶对水解液的处理效果源自于对木质素的聚合作用(通过酚羟基的自由基生成与偶合反应实现)，改变木质素的性质使其易于被脱除。因此，只要水解液中含有富含酚羟基的木质素，即可根据本发明所述条件，对其产生较好的聚合作用，并非针对特定阔叶木制浆预水解液。
(3)锰过氧化物酶对木质素的聚合作用及应用未见报道，本发明基于其对木质素的聚合作用，提出一种促进木质素分离，提高糖分纯化效率的方法，且其效果优于已被报道的漆酶与辣根过氧化物酶。
(4)本申请的操作方法简单、成本低、具有普适性，易于规模化生产。
应该指出，以下详细说明都是示例性的，旨在对本发明提供进一步的说明。除非另有指明，本发明使用的所有技术和科学术语具有与本发明所属技术领域的普通技术人员通常理解的相同含义。
如前所述，在制浆纤维原料的预水解过程当中，所得预水解液主要成分为半纤维素、木质素以及其他降解产物。木质素的存在对水解液中糖分的高值化利用产生了严重的阻碍，由此造成资源的浪费和企业经济效益的损失。因此，对水解液中糖分进行高值化利用之前，需要对木质素进行高效分离，同时还应保证糖分具有高得率。本发明提高了预水解液的糖分纯化过程中木质素的脱除效率，同时保证糖分具有较高得率，为糖分的高效分离提供借鉴与技术支持。
一种纯化阔叶木制浆预水解液中糖分的方法，具体步骤为：
(1)利用锰过氧化物酶处理预水解液；
(2)采用活性炭等吸附材料处理经酶预处理的水解液；
(3)过滤分离活性炭等吸附材料得到纯化预水解液；
本发明的方法，先利用锰过氧化物酶处理预水解液，然后采用活性炭等吸附材料吸附去除水解液中的木质素等杂质得到纯化水解液。
本发明通过先利用锰过氧化物酶处理后采用吸附材料吸附的方法，通过酶处理改变水解液中木质素的性质，聚合木质素小分子提高其分子量，使得木质素易于脱除，利用活性炭等吸附材料吸附酶处理后的水解液，提高木质素的去除效率，总木糖损失较低，是一条实现组分分离并高值利用的可行途径。
在一些实施例中，利用锰过氧化物酶处理预水解液，优选温度为20～50℃；比如可以为20℃、30℃。在一些实施例中，过氧化氢的用量为1～30mM·L-1；比
如可以为4.40、6.88mM·L-1。在一些实施例中，酶用量为50～800U·L-1，比如可以为375、487.5U·L-1。在一些实施例中，Mn2+用量为0.01～1mM·L-1，比如可以为0.25、0.5mM·L-1。在一些实施例中，反应时间为0.5～15h，比如可以为4、5h。在一些实施例中，pH为3.6～6.0，比如可以为4.5。
在一些实施例中，活性炭添加量为水解液质量的0.2～0.6wt％。在一些实施例中，活性炭进行吸附是在室温下反应，反应时间为5-15min，磁力搅拌器转速为250-350rpm。
在一些实施例中，所述植物原料为速生杨木、速生桉木、相思木等阔叶木制浆纤维原料。
上述利用锰过氧化物酶处理阔叶木制浆预水解液纯化糖分的方法制备得到的富含木糖和木聚糖的预水解液。
下面结合具体的实施例，对本发明做进一步的详细说明，应该指出，所述具体实施例是对本发明的解释而不是限定。
实施例1：
一种纯化阔叶木制浆预水解液中糖分的方法，其步骤如下：
(1)利用锰过氧化物酶对速生杨木预水解液进行处理(采用水热法处理杨木片制备预水解液，杨木片与水的质量比为1:6，预处理温度为170℃，预处理时间为60min，反应结束后固液分离，液体为预水解液，固体为水解后的木片，用于后续制浆)。反应温度为30℃，pH为4.5，酶添加量为375U·L-1，过氧化氢用量为6.88mM·L-1,Mn2+(MnSO4)用量为0.25mM·L-1，摇床转速为150rpm，反应6h后，沸水浴5min将酶灭活，终止反应。
(2)向经过锰过氧化物酶处理后的水解液中加入0.2wt％的活性炭，300rpm
搅拌10min，反应完成后过滤分离得纯化后的水解液。
(3)测定本实施例的木质素去除率及总木糖得率，列于表1。
(4)对照例1不经过酶处理，只利用0.2wt％的活性炭处理水解液，测定木质素去除率及总木糖得率，列于表1。
实施例2：
一种纯化阔叶木制浆预水解液中糖分的方法，其步骤如下：
(1)利用锰过氧化物酶处理相思木预水解液(采用水热法处理相思木片制备预水解液，相思木片与水的质量比为1:6，预处理温度为170℃，预处理时间为60min，反应结束后固液分离，液体为预水解液，固体为水解后的木片，用于后续制浆)。反应温度为30℃，pH为4.5，酶添加量为487.5U·L-1，过氧化氢用量为4.4mM·L-1,Mn2+(MnSO4)用量为0.25mM·L-1，摇床转速为150rpm，反应5h后，沸水浴5min将酶灭活，终止反应。
(2)向经过锰过氧化物酶处理后的水解液中加入0.4wt％的活性炭，300rpm搅拌10min，反应完成后过滤分离得纯化后的水解液。
(3)测定本实施例的木质素去除率及总木糖得率，列于表1。
(4)对照例2不经过酶处理，只利用0.4wt％的活性炭处理水解液，测定木质素去除率及总木糖得率，列于表1。
表1
对比例3
一种纯化阔叶木制浆预水解液中糖分的方法，其步骤如下：
(1)利用漆酶在优选条件下对速生杨木预水解液进行处理(采用水热法处理杨木片制备预水解液，杨木片与水的质量比为1:6，预处理温度为170℃，预处理时间为60min，反应结束后固液分离，液体为预水解液，固体为水解后的木片，用于后续制浆)。反应温度为35℃，pH为4.5，酶添加量为2000U·L-1，摇床转速为150rpm，反应4h后，沸水浴5min将酶灭活，终止反应。
(2)向经过漆酶处理后的水解液中加入0.2wt％的活性炭，300rpm搅拌10min，反应完成后过滤分离得纯化后的水解液。
(3)测定对比例的木质素去除率及总木糖得率，列于表1。
通过以上实例说明，水解液在经过锰过氧化物酶处理后，可以利用活性炭吸附去除更多的木质素。在反应温度为30℃，pH为4.5，酶添加量为375U·L-1水解液，过氧化氢用量为6.88mM·L-1，Mn2+用量为0.25mM·L-1，反应时间为6h条件下，利用0.2wt％的活性炭吸附可以去除速生杨水解液中65.71％的木质素，比对照例1(38.50％)提高了70.66％，而不额外造成总木糖的损失。在反应温度为30℃，pH为4.5，酶添加量为375U·L-1水解液，过氧化氢用量为4.4mM·L-1，Mn2+用量为0.25mM·L-1，反应时间为5h条件下，利用0.4wt％的活性炭吸附可以去除相思木水解液中73.15％的木质素，比对照例2(47.42％)提高了54.26％，而对总木糖的回收率影响较小。
在纯化水解液中糖分过程中，在各自的优选工艺条件下，锰过氧化物酶的作用效果要优于漆酶。
综上，锰过氧化物酶可以改性水解液中的木质素，使其易于被活性炭吸附等手段脱除，并对糖的回收率没有影响，显著提高木质素的分离效率，减少化学药剂的投入量，降低成本，同时酶是一种绿色环保的药剂，可以减轻环境负荷，有利于保护环境。
最后应该说明的是，以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述实施例所记载的技术方案进行修改，或者对其中部分进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (10)
- 一种纯化阔叶木制浆预水解液中糖分的方法，其特征在于，包括：采用锰过氧化物酶处理预水解液，得到酶预处理的水解液；采用吸附材料对所述酶预处理的水解液进行处理，固液分离，即得。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，锰过氧化物酶处理预水解液过程中，酶用量为50～800U·L-1，Mn2+用量为0.01～1mM·L-1，过氧化氢的用量为1～30mM·L-1。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，锰过氧化物酶处理预水解液的温度为20～50℃，反应时间为0.5～10h，pH为3.6～6.0。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，所述吸附材料为活性炭、沸石、炭分子筛或树脂。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，所述吸附材料的添加量为水解液质量的0.2～0.6wt％。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，吸附过程在机械搅拌条件下进行，转速为250-350rpm，反应时间为5-15min。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，阔叶木为速生杨木、速生桉木或相思木。
- 如权利要求1所述的纯化阔叶木制浆预水解液中糖分的方法，其特征在于，所述固液分离的方法为过滤。
- 权利要求1-8任一项所述的方法制备的木糖和木聚糖纯化液。
- 权利要求9所述的木糖和木聚糖纯化液在制备医疗保健类药物或者食品添加剂中的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2023213772A AU2023213772A1 (en) | 2022-01-25 | 2023-01-18 | Method for purifying sugars in pre-hydrolysis liquor of hardwood pulping |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210088371.4A CN114410707B (zh) | 2022-01-25 | 2022-01-25 | 一种纯化阔叶木制浆预水解液中糖分的方法 |
| CN202210088371.4 | 2022-01-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2023143310A1 true WO2023143310A1 (zh) | 2023-08-03 |
Family
ID=81277927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2023/072917 WO2023143310A1 (zh) | 2022-01-25 | 2023-01-18 | 一种纯化阔叶木制浆预水解液中糖分的方法 |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN114410707B (zh) |
| AU (1) | AU2023213772A1 (zh) |
| WO (1) | WO2023143310A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117491536A (zh) * | 2023-12-29 | 2024-02-02 | 中国林业科学研究院林产化学工业研究所 | 一种prc-apmp工艺制浆废水的微生物毒性物质鉴定方法 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410707B (zh) * | 2022-01-25 | 2023-08-15 | 齐鲁工业大学 | 一种纯化阔叶木制浆预水解液中糖分的方法 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103740787A (zh) * | 2014-01-17 | 2014-04-23 | 山东大学 | 一种利用阔叶木溶解浆预水解废液酶法生产低聚木糖的方法 |
| CN104448159A (zh) * | 2013-11-26 | 2015-03-25 | 柳州爱格富食品科技股份有限公司 | 含有腐殖酸的高吸水树脂的制备方法 |
| CN104894079A (zh) * | 2015-05-27 | 2015-09-09 | 华中科技大学 | 木质素降解液及制备方法以及用其降解木质素的方法 |
| CN109162135A (zh) * | 2018-09-13 | 2019-01-08 | 山东太阳纸业股份有限公司 | 一种利用阔叶木材生产溶解浆与低聚木糖的方法 |
| CN110256608A (zh) * | 2019-06-24 | 2019-09-20 | 齐鲁工业大学 | 辣根过氧化物酶处理除去植物纤维预水解液中木素的方法 |
| CN110699403A (zh) * | 2019-11-13 | 2020-01-17 | 齐鲁工业大学 | 一种提升阔叶木制浆预水解液中低聚木糖纯化效率的方法 |
| CN114410707A (zh) * | 2022-01-25 | 2022-04-29 | 齐鲁工业大学 | 一种纯化阔叶木制浆预水解液中糖分的方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI104835B (fi) * | 1997-01-14 | 2000-04-14 | Neste Chemicals Oy | Ligniinipohjaiset sideaineet lastulevyvalmistusta varten |
| JP3839940B2 (ja) * | 1997-11-26 | 2006-11-01 | 株式会社エス・ディー・エス バイオテック | 含金属処理剤による木材の処理方法およびその方法により処理された木材 |
| CN103429749B (zh) * | 2010-12-10 | 2016-12-14 | 诺维信公司 | 用于从含木素纤维素材料产生发酵产物的方法 |
| WO2013055890A1 (en) * | 2011-10-14 | 2013-04-18 | University Of Georgia Research Foundation, Inc. | Methods and compositions using lignolytic enzymes and mediators to reduce and reform lignin contents in lignocellulosic biomass |
| WO2018233559A1 (en) * | 2017-06-20 | 2018-12-27 | Novozymes A/S | PROCESS FOR INCREASING THE PERCENTAGE OF HYDROLYSAT XYLOSE |
| CN107936143B (zh) * | 2017-11-24 | 2021-03-23 | 齐鲁工业大学 | 一种植物纤维原料水解液生物处理去除木素的方法 |
| CN108411044B (zh) * | 2018-04-04 | 2021-04-30 | 齐鲁工业大学 | 一种桉木热水预水解液中木糖的超声波辅助纯化方法 |
| CN110042133B (zh) * | 2019-04-22 | 2021-06-04 | 齐鲁工业大学 | 一种生物酶协同物理化学法纯化制备杨木低聚木糖的方法 |
| CN110280217A (zh) * | 2019-06-24 | 2019-09-27 | 齐鲁工业大学 | 一种木素吸附剂、其制备方法及其应用 |
-
2022
- 2022-01-25 CN CN202210088371.4A patent/CN114410707B/zh active Active
-
2023
- 2023-01-18 WO PCT/CN2023/072917 patent/WO2023143310A1/zh active Application Filing
- 2023-01-18 AU AU2023213772A patent/AU2023213772A1/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104448159A (zh) * | 2013-11-26 | 2015-03-25 | 柳州爱格富食品科技股份有限公司 | 含有腐殖酸的高吸水树脂的制备方法 |
| CN103740787A (zh) * | 2014-01-17 | 2014-04-23 | 山东大学 | 一种利用阔叶木溶解浆预水解废液酶法生产低聚木糖的方法 |
| CN104894079A (zh) * | 2015-05-27 | 2015-09-09 | 华中科技大学 | 木质素降解液及制备方法以及用其降解木质素的方法 |
| CN109162135A (zh) * | 2018-09-13 | 2019-01-08 | 山东太阳纸业股份有限公司 | 一种利用阔叶木材生产溶解浆与低聚木糖的方法 |
| CN110256608A (zh) * | 2019-06-24 | 2019-09-20 | 齐鲁工业大学 | 辣根过氧化物酶处理除去植物纤维预水解液中木素的方法 |
| CN110699403A (zh) * | 2019-11-13 | 2020-01-17 | 齐鲁工业大学 | 一种提升阔叶木制浆预水解液中低聚木糖纯化效率的方法 |
| CN114410707A (zh) * | 2022-01-25 | 2022-04-29 | 齐鲁工业大学 | 一种纯化阔叶木制浆预水解液中糖分的方法 |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117491536A (zh) * | 2023-12-29 | 2024-02-02 | 中国林业科学研究院林产化学工业研究所 | 一种prc-apmp工艺制浆废水的微生物毒性物质鉴定方法 |
| CN117491536B (zh) * | 2023-12-29 | 2024-03-12 | 中国林业科学研究院林产化学工业研究所 | 一种prc-apmp工艺制浆废水的微生物毒性物质鉴定方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2023213772A1 (en) | 2023-09-07 |
| CN114410707A (zh) | 2022-04-29 |
| CN114410707B (zh) | 2023-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2023143310A1 (zh) | 一种纯化阔叶木制浆预水解液中糖分的方法 | |
| CN108411044B (zh) | 一种桉木热水预水解液中木糖的超声波辅助纯化方法 | |
| JP5987015B2 (ja) | バイオマス前処理 | |
| CN110256608B (zh) | 辣根过氧化物酶处理除去植物纤维预水解液中木素的方法 | |
| WO2012100375A1 (zh) | 一种秸秆半纤维素制备生物基产品及其组分全利用的方法 | |
| WO2014092873A1 (en) | Processes and apparatus for lignin separation in biorefineries | |
| CN109825542B (zh) | 一种去除热水预水解液中木素及制备低聚木糖的方法 | |
| JP7141774B2 (ja) | ポプラキシロオリゴ糖の精製液の調製方法、並びにそれによって調整されたポプラキシロオリゴ糖の精製液、キシロオリゴ糖の固体及びその用途 | |
| EP2464734A1 (en) | Apparatus and process for fermentation of biomass hydrolysate | |
| Ximenes et al. | Biological conversion of plants to fuels and chemicals and the effects of inhibitors | |
| CN110616237A (zh) | 一种汽爆植物纤维原料制备低聚木糖的方法 | |
| AU2020100246A4 (en) | Method for preparing enriched xylobiose to xylotetraose from eucalyptus hydrothermal pretreatment liquid | |
| WO2020258749A1 (zh) | 一种木素吸附剂、其制备方法及其应用 | |
| CN107936143B (zh) | 一种植物纤维原料水解液生物处理去除木素的方法 | |
| CN109796508B (zh) | 一种制备高纯低聚木糖的方法 | |
| Tang et al. | Significantly enhanced enzymatic hydrolysis of waste rice hull through a novel surfactant-based deep eutectic solvent pretreatment | |
| Yang et al. | Integrated forest biorefinery: value-added utilization of dissolved organics in the prehydrolysis liquor of prehydrolysis kraft (PHK) dissolving pulp production process | |
| CN110699403A (zh) | 一种提升阔叶木制浆预水解液中低聚木糖纯化效率的方法 | |
| Hou et al. | Selective separation of hemicellulose from poplar by hydrothermal pretreatment with ferric chloride and pH buffer | |
| CN113914125A (zh) | 一种从甘蔗渣分离纤维素并酶解的方法 | |
| CN107164431B (zh) | 一种光催化-自由基氧化预处理剑麻渣制备还原糖水解物的方法 | |
| CN111534556B (zh) | 一种利用杨木酶法制备高浓度单糖溶液的方法 | |
| He et al. | Integrated understanding of enhancing enzymatic hydrolysis of bulrush through the metal chloride-assisting choline chloride: Glycerol pretreatment | |
| CN113046400A (zh) | 一种超快速预处理生物质中木质纤维素的方法 | |
| CN114657807B (zh) | 一种利用生物酶促进木材制浆预水解液中木质素聚积的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WWE | Wipo information: entry into national phase |
Ref document number: 2023213772 Country of ref document: AU |
|
| ENP | Entry into the national phase |
Ref document number: 2023213772 Country of ref document: AU Date of ref document: 20230118 Kind code of ref document: A |
|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 23746191 Country of ref document: EP Kind code of ref document: A1 |