WO2023132120A1

WO2023132120A1 - Method for producing extract, method for producing cosmetic, and method for producing substance for oral use

Info

Publication number: WO2023132120A1
Application number: PCT/JP2022/040577
Authority: WO
Inventors: 幸雄三井
Original assignee: 株式会社ホルス
Priority date: 2022-01-07
Filing date: 2022-10-31
Publication date: 2023-07-13
Also published as: JP2023101072A; TW202329993A

Abstract

Provided are: a method for producing an extract, in which, in an extraction step for adding a starting material to a solvent to extract a useful component of the starting material, hot-spring water at a pH of 5.5 to 8.5 is used as the solvent to increase the useful component content in the extract compared to conventional methods; and a method for producing a cosmetic and a substance for oral use that contain said extract.

Description

Extract manufacturing method, cosmetic manufacturing method, and oral product manufacturing method

The present invention relates to a method for producing an extract by adding raw materials to a solvent and extracting useful ingredients, and a method for producing cosmetics or oral products containing the extract.

Hot spring water contains ingredients that are good for the skin, so it is often used not only for bathing but also in cosmetics and health foods.
For example, Patent Document 1 discloses a cosmetic containing Moor hot spring water, Moor hot spring water, Hakutsuru Reishi mushroom, Hakutsuru Reishi extract, or Hakutsuru Reishi extract powder as main ingredients for the purpose of enhancing skin moisturizing ability. It is
In addition, Patent Document 2 discloses that alkaline hot spring water, phenoxyethanol, collagen, elastin, placenta extract, pentylene glycol, glycerin, and butylene glycol are used for the purpose of increasing water retention and moisturizing power. are mixed, and the mixed liquid is alkaline.

Placenta extract extracted from placenta, which is an organ unique to mammals, contains various useful components such as amino acids and enzymes. Therefore, placenta extract is used as a raw material for cosmetics, health foods, and the like. Similarly, Rhinophyte extract extracted from umbilical cord, amniotic membrane extract extracted from amniotic membrane, sake lees extract extracted from sake lees, or bird's nest extract extracted from bird's nest are also used as raw materials for cosmetics, health foods, etc. .
For example, Patent Literature 3 discloses a food for suppressing gray hair containing a placenta extract as an active ingredient.
In addition, Patent Document 4 discloses a diet food containing a placenta extract and a rhinoceros cypress extract.
Further, Patent Document 5 discloses a method for producing an amniotic membrane-derived raw material (amniotic membrane extract) containing many components useful for anti-aging effects.
Further, Patent Document 6 discloses a gel cosmetic containing a carboxy group-containing anionic polymer, a compound of hydroxypropylmethylcellulose or xanthan gum, and sake lees extract as a skin-beautifying ingredient.
In addition, Patent Document 7 discloses a drink containing sialic acid as a bird's nest extract. It is disclosed to be formed as an extract to be extracted and contained in a beverage solvent.

In addition, Patent Document 8 discloses food and drink containing 4-vinylguaiacol, and in paragraph 0045, one of the waters that can be used as an extraction solvent when extracting 4-vinylguaiacol from plants etc. hot spring water is mentioned.
In addition, Patent Document 9 discloses an ABCA12 gene expression promoter containing a peony extract as an active ingredient. ing.

JP-A-2000-239146 JP 2012-250960 A JP 2019-054769 A JP 2006-282609 A JP 2018-188401 A JP 2019-127460 A JP 2019-129803 A JP 2020-092719 A Japanese Patent Application Laid-Open No. 2020-138932

It is desirable that extracts to be incorporated into oral products such as cosmetics or health foods contain more useful ingredients.
However, as in

Patent Documents

1 and 2, when hot spring water is mixed after extraction to form a mixture, the concentration of the extract is diluted, and the amount of useful ingredients contained in the mixture is not necessarily sufficient. There is
Moreover, Patent Documents 3 to 7 do not attempt to increase the amount of useful components contained in the extract by using hot spring water.
In addition,

Patent Documents

8 and 9 state that hot spring water may be used as an extraction solvent, but this is only an example of an extraction solvent, and focusing on hot spring water, the useful components contained in the extract can be extracted. I'm not trying to increase the quantity.
Therefore, the present invention provides a method for producing an extract that uses hot spring water to increase the content of useful components in the extract (extract, extraction solution) compared to the conventional method, thereby enhancing usefulness for the skin, and the method for producing the extract. An object of the present invention is to provide a method for producing cosmetics and oral preparations containing an extract.

A method for producing an extract corresponding to claim 1 is characterized in that hot spring water having a pH of 5.5 or more and 8.5 or less is used as a solvent in the extraction step of putting the raw material into a solvent and extracting the useful components of the raw material. and
The present invention according to claim 2 is characterized in that, in the method for producing an extract according to claim 1, the raw material is placenta, rhinoceros cypress, amniotic membrane, sake lees, or bird's nest.
The present invention according to claim 3 is the method for producing an extract according to claim 1, wherein the raw material is placenta, rhinoceros cypress, or amniotic membrane, and a freezing step of freezing the raw material before the extraction step; It is characterized by performing a normal temperature melting step of melting the raw material at normal temperature, a washing step of washing the raw material after the normal temperature melting step, and a mincing process of chopping the raw material after the washing step.
According to a fourth aspect of the present invention, in the method for producing an extract according to any one of the first to third aspects, the hot spring water is Yugawara hot spring water.
The present invention according to claim 5 is the method for producing an extract according to claim 4, wherein the Yugawara hot spring water has a sodium/calcium-chloride/sulfate hot spring and is weakly alkaline. Characterized by
A method for producing cosmetics corresponding to claim 6 is characterized in that the extract obtained by the method for producing an extract according to any one of claims 1 to 5 is blended.
A method for producing an oral preparation corresponding to claim 7 is characterized by blending an extract obtained by the method for producing an extract according to any one of claims 1 to 5.

According to the present invention, the content of useful ingredients in the extract can be increased compared to the past, and the usability for the skin can be enhanced.

A diagram showing a manufacturing process of a placenta extract (placenta extract) according to an embodiment of the present invention. Graph showing the results of component analysis of the same placenta extract Graph showing the results of the same skin moisture content test 1 Graph showing the results of the same skin moisture content test 2 Graph showing the results of component analysis of the Rhinoceros rhinoceros extract Graph showing the results of the same skin moisture content test 3 Graph showing the component analysis results of the same amniotic membrane extract (amniotic membrane extract) Graph showing the results of the same skin moisture content test 4 Graph showing the results of component analysis of the same sake lees extract (sake lees extract) Graph showing the results of the same skin moisture content test 5

Hereinafter, a method for producing an extract, a method for producing a cosmetic, and a method for producing an oral product according to embodiments of the present invention will be described.
FIG. 1 is a diagram showing the manufacturing process of a placenta extract (placenta extract).
As the raw material for the extract, various animal and plant-derived raw materials such as rhinoceros bream, amniotic membrane, sake lees, or bird's nest can be used.
First, a full-term placenta is collected immediately after delivery (S1: placenta collection step). The placentas to be collected may be those of mammals such as humans, pigs, horses or sheep, but healthy pig or horse placentas that are superior in safety and suitable for use in cosmetics and health foods should be selected. is preferred.
The collected placenta is immediately frozen (S2: first freezing step). The frozen placenta is transported to a designated location and stored. As a result, the collected placenta can be stored in good condition.
At the time of use, the frozen placenta is thawed at room temperature (S3: thawing step). The thawed placenta is washed after removing the membrane tissue (S4: washing step), and the villous tissue is chopped (S5: mincing step). In the mincing step S5, the meat is cut into pieces of a predetermined size by a cutting machine such as a mincing machine.
After the mincing step S5, the placenta is immediately placed in a freezer to freeze (S6: second freezing step). This activates the useful components contained in the placenta and improves its stability.

Next, the shredded placenta is placed in a solvent and subjected to decomposition extraction to extract useful components (S7: extraction step). Examples of the method for decomposition and extraction include enzymatic decomposition treatment, hydrolysis treatment, acid treatment, freeze-thaw treatment, and the like, and use of enzymatic decomposition treatment is particularly preferable.
Hot spring water having a pH of 5.5 to 8.5 is used as the solvent. By using hot spring water with a pH of 5.5 or more and 8.5 or less as a solvent, the useful components are extracted completely, and the useful components are contained at a higher concentration than when tap water or purified water is used as the solvent. You can get placenta extract. Further, when the pH of the hot spring water used as the solvent is 5.5 or more and 8.5 or less, pH adjustment becomes unnecessary or easy when the placenta is blended in cosmetics.

The extract (placenta extract) obtained in the extraction step S7 is heat sterilized (S8: heat sterilization step), filtered to remove impurities, and the extract is concentrated (S9: filtration step). After the filtration step S9, addition of antiseptic, inspection, filling, packaging, etc. are performed as appropriate. As a result, it can be used as a liquid raw material for placenta extract to be blended in cosmetics, health foods, and the like.
Further, by freeze-drying or spray-drying the placenta extract after the filtration step S9, it is possible to obtain a powdered raw material of the placenta extract to be blended in cosmetics, health foods, and the like.
Moreover, by blending a predetermined amount of the obtained liquid raw material or powder raw material of the placenta extract, it is possible to prepare oral products such as cosmetics or health foods containing the placenta extract.

The hot spring water used as a solvent for extraction is preferably Yugawara hot spring water, and more preferably weakly alkaline with a pH of 7.5 or more and less than 8.5. Yugawara hot spring water gushes out in and around Yugawara Town, located in the southwestern part of Kanagawa Prefecture. Yugawara hot spring water is colorless and odorless, so it can reduce the effect on color and odor when used in cosmetics, health foods, and the like.
Each example using Yugawara hot spring water as a solvent will be described below.

Example 1 is a placenta extract produced by the process shown in FIG. 1 using placenta as a raw material. An enzymatic decomposition treatment was used for the decomposition extraction method in the extraction step S7. The pH, spring quality, etc. of the Yugawara hot spring water used are as follows.
・Gush place: Miyakami, Yugawara-cho, Ashigarashimo-gun, Kanagawa Prefecture ・pH: 8.2
・Spring quality: sodium/calcium-chloride/sulfate hot spring (former spring quality name: gypsum-containing salt spring)
The results of component analysis of the placenta extract according to Example 1 are shown in Table 1 below together with the results of component analysis of the placenta extract according to Comparative Examples 1-3. Moreover, FIG. 2 is a graph of the results of Table 1. FIG. 2(a) shows free amino acids, FIG. 2(b) shows minerals, and FIG. 2(c) shows peptide molecular weight distribution.
Comparative Example 1 is a placenta extract produced in the same process as in Example 1, except that purified water was used as the extraction solvent.
Comparative Example 2 is a placenta extract produced in the same process as in Example 1, except that hot spring water from some place in the northern Kanto region was used as the extraction solvent. The pH and quality of hot spring water in Kita Kanto Somewhere are as follows.
・Gush point: Somewhere in northern Kanto ・pH: 2.0
・Spring quality: Acidic/sulfur-containing aluminum-sulfate/chloride hot spring (hydrogen sulfide type)
Comparative Example 3 is a placenta extract produced by the same process as in Example 1, except that hot spring water from a certain place in Saitama Prefecture was used as the extraction solvent. The pH and quality of hot spring water in Saitama Prefecture are as follows.
・Gush place: Somewhere in Saitama Prefecture ・pH: 8.9
・Spring quality: Sodium-chloride cold mineral spring

Comparing Example 1 (solvent: Yugawara hot spring water) with Comparative Example 1 (solvent: purified water), an increase was confirmed for all amino acids except for glycine. As for minerals, an increase was confirmed in calcium and potassium, which are expected to have cosmetic effects. Regarding the peptide molecular weight, the distribution of molecules with a size of 1,000 or more decreased, and the distribution of molecules with a size of less than 1,000, especially with a size of less than 500, which has high permeability and moisturizing effect, increased.
On the other hand, comparing Comparative Example 2 (solvent: North Kanto Somewhere Hot Spring Water) with Comparative Example 1, an increase in calcium and potassium was confirmed for minerals, but a decrease in amino acid components other than proline and cystine. Regarding the peptide molecular weight, the distribution of molecules with a size of less than 500 increased, but the distribution of molecules with a size of 500 or more and less than 3,000 decreased, and the distribution of molecules with a size of 3,000 or more increased.
Further, when comparing Comparative Example 3 (solvent: hot spring water in a certain place in Saitama Prefecture) with Comparative Example 1, it was confirmed that isoleucine, alanine, tryptophan, and cystine among the amino acids increased. are both small. As for minerals, an increase in calcium was confirmed, but a decrease in potassium. Peptide molecular weights increased in the distribution of molecules with a size of 3,000 or more and decreased in the distribution of molecules with a size of less than 3,000.
In this way, by using Yugawara hot spring water as a solvent, it is possible to increase the content of useful ingredients in the placenta extract more than when purified water or other hot spring water is used as a solvent, and the size is less than 1,000. , especially below 500 molecules can be increased.

The placenta extract of Example 1 was subjected to the following tests, and changes in skin moisture increase over time after application were measured to confirm the effect of suppressing evaporation of skin moisture.
[Skin Moisture Content Test 1]
[conditions]
Temperature: 25°C
Humidity: 25%
Measuring device: Skin surface stratum corneum water content measuring device (SKICON-200EX manufactured by Yayoi Co., Ltd.)
[sample]
Example 1: Placenta extract using Yugawara hot spring water as a solvent Comparative example 1: Placenta extract using purified water as a solvent Comparative example 4: Yugawara hot spring water Comparative example 5: Purified water [Method]
1. Mark a 3.0 cm long by 2.0 cm wide frame on the inside of the lower arm with a permanent marker.
2. Wash the measurement points with soap and rest for 30 minutes.
3. Measure the normal skin moisture content at the measurement point. In addition, it measures 5 times and calculates|requires an average value.
4. Take 0.1 g of the sample on the measurement site, and evenly apply the sample 10 times vertically and horizontally using up to the second joint of the finger.
5. 5, 10, 15, and 60 minutes after application of the sample, the skin moisture content is measured using a measuring instrument.

[Skin Moisture Content Test 2]
[conditions]
Temperature: 25°C
Humidity: 32%
Measuring device: Skin surface stratum corneum water content measuring device (SKICON-200EX manufactured by Yayoi Co., Ltd.)
[sample]
Example 1: Placenta extract using Yugawara hot spring water as a solvent Comparative example 5: Purified water Comparative example 6: A mixture of placenta extract and Yugawara hot spring water using purified water as a solvent (mixing was performed immediately before the test, the mixing ratio was 1 : 1)
[Method]
Same as Skin Moisture Content Test 1 except that measurements were made 5, 10, 15 and 60 minutes after application of the sample, and also 30 minutes after application.

FIG. 3 is a graph showing the results of Skin Moisture Content Test 1, and FIG. 4 is a graph showing the results of Skin Moisture Content Test 2. As shown in FIG. The horizontal axis represents time (min), and the vertical axis represents skin moisture content (μS), with the normal skin moisture content set to 0.
In FIG. 3 , the results of Example 1 are indicated by “● and solid line”, the results of Comparative Example 1 are indicated by “▲ and long dashed line”, the results of Comparative Example 4 are indicated by “× and dotted line”, and Comparative Example 5. The results are indicated by “♦ and short dashed line”. As a result of Skin Moisture Content Test 1, the placenta extract of Example 1 exhibited a higher skin moisture content than each Comparative Example immediately after application, and exhibited a higher skin moisture content than each Comparative Example even 60 minutes after application.
In FIG. 4 , the results of Example 1 are indicated by “● and solid line”, the results of Comparative Example 5 are indicated by “♦ and long dashed line”, and the results of Comparative Example 6 are indicated by “▲ and short dashed line”. As a result of the skin moisture content test 2, the placenta extract of Example 1 had the same skin moisture content as the placenta extract of Comparative Example 6 until about 10 minutes after application, but the subsequent decrease in skin moisture content was comparable. Compared to Example 6, it was moderate, and even after 60 minutes of application, the skin moisture content was higher than that of each comparative example.

As described above, according to the production method of the present invention, it is possible to obtain a placenta extract that has a higher content of useful ingredients, an enhanced usefulness to the skin, and an excellent moisturizing effect.
In addition, even when materials other than placenta, such as rhinoceros phlox, amniotic membrane, sake lees, or bird's nest, are used as raw materials, the production method of the present invention can be used to increase the useful ingredients and the moisturizing effect. The following shows the test results when the raw materials were rhinoceros rotunda, amniotic membrane, or sake lees.

Example 2 is a rhinoceros rotunda extract produced using rhinoceros rotunda (umbilical cord) as a raw material. The production method is basically the same as the placenta extract production method of Example 1, but does not have the second freezing step S6. Enzymatic decomposition treatment was used as the method of decomposition extraction in the extraction process. The pH and spring quality of the Yugawara hot spring water used are the same as in Example 1.
The results of component analysis of the Rhinoceros rotundus extract (Rhinoceros rhinoceros rotunda extract) according to Example 2 are shown in Table 2 below together with the results of component analysis of the Rhinoceros rhinoceros rotundum extract according to Comparative example 7. Moreover, FIG. 5 is a graph of the results of Table 2, FIG. 5(a) is the mineral, and FIG. 5(b) is the polysaccharide molecular weight distribution.
Comparative Example 7 is a rhinoceros cypress extract produced in the same process as in Example 2, except that purified water was used as the extraction solvent.

Comparing Example 2 (solvent: Yugawara hot spring water) with Comparative Example 7 (solvent: purified water), a significant increase in calcium, which is expected to have cosmetic effects, was confirmed. Regarding polysaccharide molecular weight, the distribution of molecules with a size of 10,000 or more decreased, and the distribution of molecules with a size of 1,000 or more and less than 10,000, which has high permeability and moisturizing effect, increased.
Thus, by using Yugawara hot spring water as a solvent, it is possible to increase the content of useful components in the rhinoceros cypress extract as compared with the case where purified water is used as a solvent.

The following test was performed on the Rhinoceros californica extract of Example 2, and changes in the increase in skin moisture over time after application were measured to confirm the effect of suppressing evaporation of skin moisture.
[Skin moisture content test 3]
[conditions]
Temperature: 24°C
Humidity: 31%
Measuring device: Skin surface stratum corneum water content measuring device (SKICON-200EX manufactured by Yayoi Co., Ltd.)
[sample]
Example 2: Saitai extract using Yugawara hot spring water as a solvent Comparative example 5: Purified water Comparative example 8: A mixture of Saitai extract and Yugawara hot spring water using purified water as a solvent (mixing was performed immediately before the test, mixing ratio is 1:1)
[Method]
Same as skin moisture content test 2.

6 is a graph showing the results of Skin Moisture Content Test 3. FIG. The horizontal axis represents time (min), and the vertical axis represents skin moisture content (μS), with the normal skin moisture content set to 0.
In FIG. 6 , the results of Example 2 are indicated by “● and solid line”, the results of Comparative Example 5 are indicated by “♦ and long dashed line”, and the results of Comparative Example 8 are indicated by “▲ and short dashed line”. As a result of Skin Moisture Content Test 3, the Rhinoceros magpita extract of Example 2 showed a slower decrease in skin moisture content after 15 minutes of application than each Comparative Example, and even after 60 minutes of application, the skin moisture content was higher than that of each Comparative Example. Moisture content was indicated.

Example 3 is an amniotic membrane extract produced using amniotic membrane as the raw material. The production method is basically the same as the placenta extract production method of Example 1, but does not have the second freezing step S6. Enzymatic decomposition treatment was used as the method of decomposition extraction in the extraction process. The pH and spring quality of the Yugawara hot spring water used are the same as in Example 1.
The results of component analysis of the amniotic membrane extract (amniotic membrane extract) according to Example 3 are shown in Table 3 below together with the results of component analysis of the amniotic membrane extract according to Comparative Example 9. Moreover, FIG. 7 is a graph of the results of Table 3, where FIG. 7(a) shows free amino acids, FIG. 7(b) shows minerals, and FIG. 7(c) shows peptide molecular weight distribution.
Comparative Example 9 is an amniotic membrane extract produced in the same steps as in Example 3, except that purified water was used as the extraction solvent.

Comparing Example 3 (solvent: Yugawara hot spring water) with Comparative Example 9 (solvent: purified water), an increase in all 18 kinds of amino acids was confirmed. As for minerals, an increase was confirmed in calcium, potassium, and magnesium, which are expected to have cosmetic effects. As for the peptide molecular weight, the distribution of molecules with a size of 1,000 to 6,000 decreased significantly, and the distribution of molecules with a size of less than 500, which has high permeability and moisturizing effect, increased.
Thus, by using Yugawara hot spring water as a solvent, it is possible to increase the content of useful components in the amniotic membrane extract and increase the molecular distribution of less than 500 compared to the case of using purified water as a solvent. be able to.

The amniotic membrane extract of Example 3 was subjected to the following tests, and changes in skin moisture increase over time after application were measured to confirm the skin moisture evaporation inhibitory effect.
[Skin Moisture Content Test 4]
[conditions]
Temperature: 25°C
Humidity: 41%
Measuring device: Skin surface stratum corneum water content measuring device (SKICON-200EX manufactured by Yayoi Co., Ltd.)
[sample]
Example 3: Amniotic membrane extract using Yugawara hot spring water as a solvent Comparative example 5: Purified water Comparative example 10: A mixture of amniotic membrane extract and Yugawara hot spring water using purified water as a solvent (mixing was performed immediately before the test, the mixing ratio was 1 : 1)
[Method]
Same as skin moisture content test 2.

8 is a graph showing the results of Skin Moisture Content Test 4. FIG. The horizontal axis represents time (min), and the vertical axis represents skin moisture content (μS), with the normal skin moisture content set to 0.
In FIG. 8 , the results of Example 3 are indicated by “● and solid line”, the results of Comparative Example 5 are indicated by “♦ and long dashed line”, and the results of Comparative Example 10 are indicated by “▲ and short dashed line”. As a result of skin moisture content test 4, the amniotic membrane extract of Example 3 exhibited a higher skin moisture content than each comparative example immediately after application, and exhibited a higher skin moisture content than each comparative example even 60 minutes after application.

Example 4 is a sake lees extract produced using sake lees as the raw material. The sake lees extract of Example 4 was extracted by adding 1,3-butylene glycol to the solvent containing the sake lees and stirring the mixture periodically for a predetermined period of time. The pH and spring quality of Yugawara hot spring water used as a solvent are the same as in Example 1.
The results of component analysis of the sake lees extract (sake lees extract) according to Example 4 are shown in Table 4 below together with the results of component analysis of the sake lees extract according to Comparative Example 11. Moreover, FIG. 9 is a graph of the results of Table 4. FIG. 9(a) is for amino acids, FIG. 9(b) is for vitamins, and FIG. 9(c) is for minerals.
Comparative Example 11 is a sake lees extract produced in the same process as in Example 4, except that purified water was used as the extraction solvent.
Sake lees is extracted by a different method from placenta-based raw materials such as placenta, and the original protein content is low. Specifically, in the case of an extract made from a placental system, the free amino acids in the extract are measured as they are, whereas in the case of sake lees extract, the amino acids are measured after all the proteins in the extract are hydrolyzed. . In addition, in the case of extracts made from placenta as raw materials, only those that function as amino acids are measured, whereas in the case of sake lees extract, they are contained regardless of whether they function as amino acids. The difference is that all amino acids are measured.

Comparing Example 4 (solvent: Yugawara hot spring water) with Comparative Example 11 (solvent: purified water), an increase was confirmed in 11 out of 18 amino acids. As for minerals, an increase was confirmed in calcium and potassium, which are expected to have cosmetic effects. As for vitamins, an increase was confirmed in vitamin B6 and niacin, which are expected to have cosmetic effects.
By using Yugawara hot spring water as a solvent in this manner, the content of useful components in the sake lees extract can be increased more than when purified water is used as a solvent.

The sake lees extract of Example 4 was subjected to the following tests, and changes in skin moisture increase over time after application were measured to confirm the effect of suppressing evaporation of skin moisture.
[Skin Moisture Content Test 5]
[conditions]
Temperature: 25°C
Humidity: 31%
Measuring device: Skin surface stratum corneum water content measuring device (SKICON-200EX manufactured by Yayoi Co., Ltd.)
[sample]
Example 4: Sake lees extract using Yugawara hot spring water as the solvent Comparative example 5: Purified water Comparative example 11: Sake lees extract using purified water as the solvent Comparative example 12: Mixture of sake lees extract and Yugawara hot spring water using purified water as the solvent (Mixing is done just before the test, the mixing ratio is 1:1)
[Method]
Same as skin moisture content test 2.

10 is a graph showing the results of Skin Moisture Content Test 5. FIG. The horizontal axis represents time (min), and the vertical axis represents skin moisture content (μS), with the normal skin moisture content set to 0.
In FIG. 10 , the results of Example 4 are indicated by “● and solid line”, the results of Comparative Example 5 are indicated by “◆ and short dashed line”, the results of Comparative Example 11 are indicated by “× and dotted line”, and Comparative Example 12. The results are indicated by “▲ and long dashed line”. As a result of skin moisture content test 5, the sake lees extract of Example 4 exhibited a higher skin moisture content than each comparative example immediately after application, and exhibited a higher skin moisture content than each comparative example even 60 minutes after application.

S1 placenta collection step S2 first freezing step (freezing step)
S3 Thawing step S4 Washing step S5 Mincing step S6 Second freezing step (freezing step)
S7 Extraction step S8 Heat sterilization step S9 Filtration step

Claims

A method for producing an extract, wherein hot spring water having a pH of 5.5 or more and 8.5 or less is used as the solvent in the extraction step of putting the raw material into a solvent and extracting the useful components of the raw material.
　The method for producing the extract according to claim 1, wherein the raw material is placenta, rhinoceros cypress, amniotic membrane, sake lees, or bird's nest.
The raw material is placenta, rhinoceros tie, or amniotic membrane,
Before the extraction step,
a freezing step of freezing the raw material;
A room temperature melting step of melting the raw material at room temperature after the freezing step;
a washing step of washing the raw material after the step of melting at room temperature;
2. The method for producing an extract according to claim 1, further comprising performing a mincing step of shredding the raw material after the washing step.
The method for producing the extract according to any one of claims 1 to 3, wherein the hot spring water is Yugawara hot spring water.
The method for producing an extract according to claim 4, wherein the Yugawara hot spring water has a sodium/calcium-chloride/sulfate hot spring and is weakly alkaline.
A method for producing cosmetics, characterized by blending the extract obtained by the method for producing an extract according to any one of claims 1 to 5.
A method for producing an oral preparation, characterized by blending the extract obtained by the method for producing an extract according to any one of claims 1 to 5.