CN116440024B

CN116440024B - Sea collagen acne-removing repair cream from sea cucumber

Info

Publication number: CN116440024B
Application number: CN202310694182.6A
Authority: CN
Inventors: 周莹
Original assignee: Shenzhen Alpha Biotechnology Co ltd
Current assignee: Shenzhen Alpha Biotechnology Co ltd
Priority date: 2023-06-13
Filing date: 2023-06-13
Publication date: 2023-10-17
Anticipated expiration: 2043-06-13
Also published as: CN116440024A

Abstract

The invention provides sea collagen acne-removing repair cream from sea cucumbers, which relates to the field of repair cosmetics and comprises the following components in percentage by mass: 1% -6% of marine collagen, 5% -10% of humectant, 5% -10% of emollient, 5% -10% of emulsifier, 0.5% -5% of emulsion stabilizer, 0.1% -0.5% of titanium dioxide, 0.5% -5% of natural squalane, 0.1% -0.5% of sodium hyaluronate, 1% -15% of skin conditioner, 0.1% -5% of antioxidant, 0.05% -1% of thickener, 0.1% -0.5% of pH regulator, 0.1% -1% of preservative, 0.01% -0.1% of antibacterial agent, 0.01% -0.05% of essence and spice, 0.05% -0.1% of EDTA-2Na and the balance of water.

Description

Sea collagen acne-removing repair cream from sea cucumber

Technical Field

The invention relates to the field of repairing cosmetics, in particular to sea collagen acne-removing repairing cream from sea cucumbers.

Background

The acne mark and the acne pit are scar pieces with different sizes, namely pigmentation and scar left after the inflammation of the sebaceous gland of the hair follicle, which are left after the healing of the facial acne. Some scars can self-repair over time, while most orange-peel-like scars are difficult to self-repair, requiring effective intervention. The existing more effective method for repairing facial scars mainly comprises the steps of drug treatment, lattice laser treatment, microneedle treatment and the like. The medicine treatment generally comprises western medicines and traditional Chinese medicine treatment, wherein the western medicines mainly comprise tretinoin or fruit acid, salicylic acid, benzoyl peroxide and the like which are mainly stripped, and have certain irritation. Traditional Chinese medicine treatment generally needs to be matched with sufficient sleep, balanced and light diet, and has good effect but long treatment period. Laser dot matrix and microneedle therapy are generally used to cause certain damage to the face and other stimulation to the skin. In addition, the above method has better effect on the newly generated lighter scar, but has little effect on the old scar, and even has certain side effect.

Therefore, there is a need for a product capable of removing acne and repairing old scars, in which marine collagen has effects of improving skin texture, moisturizing, etc., and can significantly delay aging, and cosmetics using marine collagen as a repairing ingredient have been used gradually at present. For example, chinese patent application publication No. CN109106665a discloses a marine organism peptide acne-removing repairing mask and a preparation method thereof, which adopts polypeptide composition, dipotassium glycyrrhizinate and aloe freeze-dried powder as main active ingredients, and can play a role in repairing skin to a certain extent. The Chinese patent application with publication number of CN106726669A discloses an anti-aging cosmetic matrix containing sea cucumber polypeptide and a preparation method thereof, which adopts sea cucumber polypeptide hydrolysate, ganoderan and ginseng root extract as effective components, can effectively improve the absorbability of skin, and has a certain wrinkle-removing effect for providing nutrition to skin.

However, the above products using marine collagen as a main component have poor selectivity, single property, difficult provision of effective skin repair, excessive use of chemicals for proportioning, and certain side effects, and the existing products have insufficient nutrition supply to skin in acne areas during use, and difficult recovery of skin to original state after acne removal. In addition, the efficiency of extracting the marine collagen protein in the prior art is lower, so that the product cost is higher and the economic benefit is poor.

Disclosure of Invention

The invention provides sea collagen acne-removing repair cream from sea cucumbers, which mainly comprises sea collagen extracted from sea cucumbers, and is supplemented with components such as skin conditioning agents extracted from natural components, so that the acne-removing repair cream can play a role in repairing facial skin, can provide sufficient amino acids, has no irritation to the skin, and has high extraction efficiency.

The invention provides sea collagen acne-removing repair cream from sea cucumbers, which comprises the following components in percentage by mass: 1% -6% of marine collagen, 5% -10% of humectant, 5% -10% of emollient, 5% -10% of emulsifier, 0.5% -5% of emulsion stabilizer, 0.1% -0.5% of titanium dioxide, 0.5% -5% of natural squalane, 0.1% -0.5% of sodium hyaluronate, 1% -15% of skin conditioner, 0.1% -5% of antioxidant, 0.05% -1% of thickener, 0.1% -0.5% of pH regulator, 0.1% -1% of preservative, 0.01% -0.1% of antibacterial agent, 0.01% -0.05% of essence and spice, 0.05% -0.1% of EDTA-2Na and the balance of water, wherein the marine collagen is I-type marine collagen extracted from sea cucumbers, and the I-type marine collagen is prepared by the following method:

S1, extracting collagen from sea cucumbers to obtain type I marine collagen macromolecule stock solution;

and S2, performing secondary refining on the type I marine collagen macromolecule stock solution extracted in the step S1 to obtain type I marine collagen.

Further, the step S1 includes:

s1-1, soaking the sea cucumber until no hard core exists, cutting open the belly of the sea cucumber, removing a sand nozzle, peeling off the viscera of the sea cucumber from the sea cucumber body, and cleaning the viscera of the sea cucumber and the sea cucumber body respectively;

step S1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the crushed sea cucumber body into an aqueous solution of acetic acid and xylitol for soaking, adding pepsin for reaction for 8-10 hours, extracting at 4 ℃ for 50 hours, adjusting the pH value to 7.0-7.5, centrifuging to remove sediment, adding ethanol to supernatant to precipitate polysaccharide, and collecting supernatant, namely sea cucumber body collagen liquid;

step S1-3, crushing the sea cucumber viscera washed in the step S1-1, adding a first compound protease, adjusting the pH value to 8.0-9.0, reacting for 1-3 hours, adjusting the pH value of the enzymolysis liquid to 7.0-7.5, and centrifugally separating to obtain supernatant fluid, thus obtaining the sea cucumber viscera collagen liquid;

s1-4, mixing the sea cucumber body collagen liquid and the sea cucumber viscera collagen liquid to obtain a collagen mixed liquid;

S1-5, adding sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.0-7.5, then adding NaCl solution with the concentration of 5.1mol/L until the concentration of NaCl is 1.7mol/L, standing for 10-24 hours after stirring, centrifuging the solution, adding 4.0mol/L NaCl solution into the supernatant until the concentration of NaCl is 2.5mol/L, standing for 10-24 hours after stirring, centrifuging again, dissolving precipitate in 1% acetic acid solution, dialyzing 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 10-12 times, and finally obtaining the type I marine collagen macromolecule stock solution, wherein the pH value of the solution is equal to that of the collagen mixed solution

The step S2 includes:

step S2-1, uniformly mixing the type I marine collagen macromolecule stock solution and a sodium acetate buffer solution according to a ratio of 1:3, regulating the pH value to 7.5-8.5, adding a second compound protease, reacting for 4 hours at 40 ℃, inactivating the second compound protease by boiling water bath, centrifuging the mixed solution, taking the supernatant, concentrating the supernatant, and obtaining the type I marine collagen, wherein the step comprises the steps of

In the step S1-2, the molar ratio of the acetic acid to the xylitol in the aqueous solution of the acetic acid and the xylitol is 1:1.

Further, the first complex protease comprises papain, pepsin, and Flavourzyme protease; the second complex protease includes alkaline protease and trypsin.

Further, the moisturizer comprises the following components: glycerol, propylene glycol and 1, 2-hexanediol; the emollient includes butylene glycol, isopropyl myristate, isohexadecane, coconut oil, butter fruit and mineral oil.

Further, the skin conditioner comprises moringa seed oil, mango seed oil and royal jelly.

Further, the antioxidants include natural vitamin E and astaxanthin oil.

Further, the emulsifier comprises the following components: glyceryl monostearate, tween 60, tween 80, span 60, EG emulsifier and stearyl alcohol polyether-21; the emulsion stabilizer comprises cetyl alcohol/stearyl alcohol.

Further, the antimicrobial agent comprises the following components: the water-soluble cool sensing agent and the plant-source bacteriostatic agent, wherein the preservative comprises phenoxyethanol.

Further, the thickener comprises carbomers; the pH adjuster comprises arginine.

According to the second aspect of the invention, the sea collagen acne-removing repair cream from sea cucumbers comprises the following components in percentage by mass: 1% -6% of marine collagen, 1% -5% of glycerol, 1% -5% of propylene glycol, 0.1% -1% of 1, 2-hexanediol, 1% -5% of butylene glycol, 1% -5% of isopropyl myristate, 1% -5% of isohexadecane, 0.5% -5% of coconut oil, 0.5% -5% of shea butter, 0.5% -5% of mineral oil, 1% -5% of glyceryl monostearate, 1% -5% of tween 60, 0.1% -1% of tween 80, 1% -5% of span 60, 0.1% -1% of EG emulsifier, 0.1% -1% of stearyl alcohol polyether-21, 0.5% -5% of cetyl alcohol/stearyl alcohol mixture, 0.1% -0.5% of sodium hyaluronate, 0.5% -5% of moringa seed oil, 0.5% -5% of mango seed oil, 0.5% -5% of royal jelly, 0.5% of vitamin E, 0.5% -5% of green oil, 0.05% of marine oil, 0.01% -1% of marine collagen, and 0.01% -1% of vegetable oil, wherein:

The sea collagen acne-removing repair cream provided by the invention adopts the sea collagen, and through the type I collagen extracted from sea cucumber, sufficient amino acid required by wound healing can be provided in the acne-removing process, the concentration and activity of the amino acid are improved, the type I sea collagen can stimulate the reconstructed collagen scaffold, the regeneration and arrangement of collagen fibers and elastic fibers in the dermis layer can be promoted in the repair process, the existing inflammatory pigmentation can be dissipated, the generation of the neuraminidase is inhibited, the new pigmentation is prevented, the repair effect on scars is good, and the long-term protection effect can be generated.

In the extraction process of the marine collagen used in the sea cucumber-derived marine collagen acne-removing repair cream, firstly, the collagen in the sea cucumber is extracted by the compound protease with different components, the type I collagen macromolecule stock solution can be obtained in the primary extraction process, and the type I marine collagen which has smaller molecular weight and is easy to be absorbed and utilized by human skin can be obtained in the secondary extraction process. The marine collagen adopts a secondary refining extraction method, and in the primary extraction process, viscera and flesh of the sea cucumber are treated separately, so that the problem of low extraction rate of mixed treatment is avoided, the integral utilization rate of the sea cucumber is improved, the extraction rate and purity of the marine collagen can be improved, and meanwhile, polysaccharides in the sea cucumber are removed, so that the nutrition supply amount of the marine collagen to skin can be improved, and the cost is effectively reduced. The extraction rate of the type I marine collagen can reach 30%, the molecular weight of the type I marine collagen is 1000Da-150kDa, and the type I marine collagen is rich in proline and hydroxyproline, and can be beneficial to the absorption and utilization of skin.

In addition, the sea collagen acne-removing repair cream of the sea cucumber source adopts a natural skin conditioner, the added antioxidant is natural vitamin E and astaxanthin oil, and the bacteriostatic agent is added with a plant-source bacteriostatic agent, does not adopt irritant ethanol, and does not generate irritation to human bodies. The composition has effects of diminishing inflammation, inhibiting bacteria, removing acne, repairing pigmentation and scar left after acne caused by inflammation of sebaceous gland, and repairing old scar. The natural components are used, so that the skin can be promoted to absorb marine collagen without stimulation, the water-oleic acid-alkali balance of the skin can be regulated, the inflammation is inhibited, the skin is protected for a long time, and the acne-removing repairing effect is achieved.

Drawings

Fig. 1 is a graph showing comparison of red spot number and total spot area test values of marine collagen acne removing cream from sea cucumber sources, which is tested before use and at 14 th day of use.

Fig. 2 is a graph comparing the percutaneous moisture lapse test values of marine collagen anti-acne cream derived from sea cucumber according to the present invention, which was tested before and at 14 days of use.

Fig. 3 is a picture of an acute skin irritation test of sea cucumber-derived marine collagen anti-acne repair cream according to the present invention on rabbits.

Detailed Description

Reference will now be made in detail to various embodiments of the invention, examples of which are illustrated in the accompanying drawings and described below. While the invention will be described in conjunction with the exemplary embodiments thereof, it will be understood that the present description is not intended to limit the invention to those exemplary embodiments. On the other hand, the present invention is intended to cover not only the exemplary embodiments of the present invention, but also various alternatives, modifications, equivalents and other embodiments, which may be included within the spirit and scope of the invention as defined by the appended claims.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. The specific structures and functions described in the exemplary embodiments of the present invention are for illustrative purposes only. Embodiments of the inventive concept according to the present invention may be embodied in various forms and it should be understood that they should not be construed as limited to the exemplary embodiments described in the exemplary embodiments, but include all modifications, equivalents, or alternatives falling within the spirit and scope of the invention.

Throughout the specification, the terminology used herein is for the purpose of describing various exemplary embodiments only and is not intended to be limiting. It will be further understood that the terms "comprises," "comprising," "includes," "including" and the like, when used in this exemplary embodiment, specify the presence of stated features, steps, operations, or elements, but do not preclude the presence or addition of one or more other features, steps, operations, or elements.

According to an aspect of the present invention, there is provided a marine collagen acne-removing repair cream (hereinafter referred to as "acne-removing repair cream" for convenience of description) derived from sea cucumber, the acne-removing repair cream comprising the following components in mass percent: 1% -6% of marine collagen, 5% -10% of humectant, 5% -10% of emollient, 5% -10% of emulsifier, 0.5% -5% of emulsion stabilizer, 0.1% -0.5% of titanium dioxide, 0.5% -5% of natural squalane, 0.1% -0.5% of sodium hyaluronate, 1% -15% of skin conditioner, 0.1% -5% of antioxidant, 0.05% -1% of thickener, 0.1% -0.5% of pH regulator, 0.1% -1% of preservative, 0.01% -0.1% of antibacterial agent, 0.01% -0.05% of essence and spice, 0.05% -0.1% of EDTA-2Na and the balance of water. The rest water is 100% by weight of the total water added on the basis of the other components, and in addition, the water used in the invention is cosmetic-grade deionized water. The marine collagen in the acne-removing repair cream provided by the invention is I-type marine collagen extracted from sea cucumbers.

The sea collagen acne-removing repair cream from sea cucumbers provided by the invention adopts a natural skin conditioner, is added with various plant and animal essences, does not adopt a irritant substance, is compounded with various components, and has the effects of diminishing inflammation, inhibiting bacteria and removing acnes. The I-type marine collagen is extracted from sea cucumbers, 80% of the collagen content in the skin of a human body is I-type collagen, and the I-type collagen has large tissue components and compact arrangement and can play a role in shaping and supporting, so that the I-type marine collagen extracted from the sea cucumbers can be directly used for the skin of the human body and play a role in repairing and supporting the skin. Preferably, in order to most effectively exert the effect of the type I marine collagen according to the present invention, the type I marine collagen is preferably added in an amount of 4% or 5% of the type I marine collagen, i.e., 4% or 5% of the total mass of the type I marine collagen.

Further, the type I marine collagen of the present invention is prepared by the following method: s1, extracting collagen from sea cucumbers so as to obtain type I marine collagen macromolecule stock solution; s2, performing secondary refining on the type I marine collagen macromolecule stock solution extracted in the step S1 to obtain the type I marine collagen. In the step S1, the marine collagen is extracted into the type I marine collagen macromolecule stock solution, and the type I marine collagen macromolecule stock solution is subjected to secondary refining processing, so that the type I marine collagen macromolecule stock solution is subjected to enzymolysis to obtain the marine collagen macromolecule stock solution with the molecular weight of 1000Da to 150kDa, the collagen has high selectivity and strong pertinence, is favorable for skin absorption, can select amino acids required by skin, and provides amino acids favorable for skin regeneration, such as proline, hydroxyproline, alanine, leucine, glycine, serine and the like, thereby playing a repairing role.

In addition, the sea cucumber is selected according to the characteristics and components of sea cucumbers in different sea areas, 1400 non-toxic sea cucumber varieties are analyzed, and the sea cucumbers used in the invention are all selected from non-edible non-toxic sea cucumber varieties in pollution-free sea areas. In contrast, most of sea cucumbers selected in the preparation process of various sea cucumber products on the market at present are edible varieties, so that the raw material cost is greatly increased, and the selectivity of the sea cucumber varieties is greatly improved and the production cost is greatly reduced by exploring non-edible and non-toxic sea cucumber varieties in high-quality sea areas. In addition, the protein content of the sea cucumber variety selected by the invention is high, the extractable high-quality protein content of the sea cucumber variety reaches more than one hundred times of the collagen yield in the prior art, the production efficiency is improved, and the cost is further optimized.

The method of extracting collagen from the sea cucumber and secondarily refining the extracted collagen will be described in detail.

In some embodiments of the present invention, the step S1 of extracting collagen from sea cucumber comprises the steps of:

s1-1, soaking sea cucumbers until no hard core exists, cutting open the abdomen of the sea cucumbers, removing sand nozzles, peeling viscera and sea cucumbers, and cleaning the viscera and the sea cucumbers respectively; s1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the crushed sea cucumber body into an aqueous solution with the concentration of acetic acid and xylitol, soaking the sea cucumber body in the aqueous solution, adding pepsin for reaction for 8-10 hours, extracting the sea cucumber body at the temperature of 4 ℃ for 50 hours, adjusting the pH value to 7.0-7.5, centrifuging the sea cucumber body to remove sediment, adding ethanol sediment polysaccharide into supernatant, and collecting the supernatant to obtain sea cucumber body collagen liquid; s1-3, crushing the sea cucumber viscera washed in the step S1-1, adding a first compound protease, adjusting the pH value to 8.0-9.0, reacting for 1-3 hours, adjusting the pH value of the enzymolysis solution to 7.0-7.5, and centrifugally separating to obtain a supernatant fluid, thus obtaining the sea cucumber viscera collagen solution; s1-4, mixing the sea cucumber body collagen liquid and the sea cucumber viscera collagen liquid to obtain a collagen mixed liquid; s1-5, adding a sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.0-7.5, then adding a NaCl solution with the concentration of 5.1mol/L until the concentration of NaCl in a system is 1.7mol/L, standing for 10-24 hours after stirring, centrifuging the solution, taking a supernatant, adding a NaCl solution with the concentration of 4.0mol/L until the concentration of NaCl in the system is 2.5mol/L, standing for 10-24 hours after stirring, centrifuging again, dissolving the precipitate in a 1% acetic acid solution, dialyzing the 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 10-12 times, and finally obtaining the type I marine collagen macromolecule stock solution.

In the method for extracting the type I marine collagen macromolecular stock solution in the sea cucumber, the steps S1-1 to S1-4 are the primary extraction process of the collagen in the sea cucumber. In the prior art, sea cucumber is usually subjected to crushing enzymolysis treatment directly after sand nozzles are removed by soaking, however, because the sea cucumber bodies and viscera of the sea cucumber have different tissue structures and different protein contents, the same enzyme is adopted to treat the sea cucumber bodies and viscera, so that the treatment degrees of the sea cucumber bodies and viscera are different, and further, part of protein in the sea cucumber cannot be extracted to be wasted, and the extraction efficiency of the follow-up collagen is affected. The invention separates viscera and sea cucumber bodies of sea cucumber, adopts different enzymes to carry out enzymolysis treatment, uses pepsin to treat the sea cucumber bodies, and then uses compound protease (first compound protease) to treat the viscera of sea cucumber, and in the actual operation process, the two steps can be carried out in two reactors at the same time, thus not only saving the operation time, but also improving the extraction efficiency of collagen in the sea cucumber, and greatly improving the utilization rate of the sea cucumber, thereby producing higher economic value.

Furthermore, in step S1-2, the molar ratio of acetic acid to xylitol in the aqueous solution of acetic acid and xylitol should be 1:1, and the total mass of both acetic acid and xylitol may be 20% of the total mass of the solution. In the process, acetic acid and xylitol are mixed according to a certain proportion to form a deep eutectic solvent, and the deep eutectic solvent can promote precipitation of a large amount of organic components, so that swelling of fibers and proteins is obviously improved, and collagen molecules in sea cucumber bodies are extracted in a large amount. And acetic acid and xylitol are common food grade additives, which can not harm human bodies, and can improve the yield and reduce the cost.

After the sea cucumber body is soaked in the aqueous solution of acetic acid and xylitol, pepsin is used for treatment, so that the terminal peptide of the collagen can be hydrolyzed, the triple helix structure of the collagen can not be damaged, and complete collagen molecules can be obtained.

Further, in the processing of the viscera of sea cucumber, a first complex protease is used, which in some embodiments of the application may comprise papain, pepsin and Flavourzyme protease, i.e. a mixture of these three enzymes. The complex protease can thoroughly decompose viscera of sea cucumber into micromolecular protein, is not only beneficial to absorption of human body in the subsequent use process, but also can directly eliminate fishy smell of sea cucumber, and does not need to perform odor removal treatment on sea cucumber in the subsequent processing process.

And the step S1-5 is used for further processing the mixed sea cucumber body collagen liquid and sea cucumber viscera collagen liquid, so that the marine collagen extracted from the sea cucumber in the previous step is further separated and extracted, and the type I marine collagen stock solution is obtained. Because the solubility of different collagens, namely, type I collagen, type II collagen and type III collagen in NaCl solution is different, the three collagens in protein can be separated by controlling the concentration of the NaCl solution, after the concentration of NaCl in the mixture is regulated to 1.7mol/L by adopting 5.1mol/L NaCl solution, the type III collagen in the mixture is separated out, the sediment is discarded, and then 4.0mol/L NaCl solution is added until the concentration is 2.5mol/L, so that the type I collagen is separated out, at the moment, the type I collagen is repeatedly dissolved in 1% acetic acid solution and dialyzed by the 1% acetic acid, thereby the small molecular weight type I marine collagen stock solution can be prepared, amino acid required for wound healing can be provided for human skin, the concentration and activity of amino acid can be improved, the neogenesis and the rearrangement of collagen fibers and elastic fibers in dermis can be promoted, a good repairing effect on scars can be provided, and the skin can play a role in supporting and protecting the skin.

Further, after extracting the type I marine collagen stock solution from sea cucumber, the obtained type I marine collagen needs to be subjected to secondary refining processing, so that the collagen which can be absorbed and utilized by human skin is further extracted. For this, the step S2 may include: s2-1, uniformly mixing the type I marine collagen macromolecule stock solution and a sodium acetate buffer solution according to a ratio of 1:3, regulating the pH value to 7.5-8.5, adding a second compound protease, reacting for 4 hours at 40 ℃, inactivating the second compound protease by boiling water bath, centrifuging the mixed solution, taking supernatant, concentrating the supernatant, and obtaining the type I marine collagen.

The second complex protease used in step S2-1 may include alkaline protease and trypsin, and the type I marine collagen obtained in the step of obtaining collagen having a molecular weight of 1000Da to 150kDa is absorbable and utilizable by the human body by further subjecting the type I marine collagen to enzymolysis using a second complex protease having a different composition from the first complex protease. By adopting secondary refining (namely step S2), the invention leads the macromolecular stock solution of the type I marine collagen to be finally changed into the type I marine collagen with smaller molecular weight, thereby avoiding the problem of difficult absorption of skin caused by higher molecular weight. The type I marine collagen after secondary refining is rich in proline and hydroxyproline, and has good promotion effect on skin repair.

In order to facilitate preservation and mixing with other components, step S2 may actually further include step S2-2, namely, preparing a type I marine collagen solution from the type I marine collagen obtained in step S2-1, recording the mass percentage of the type I marine collagen in the solution, and then directly calculating the addition amount of the solution according to the mass percentage of the type I marine collagen in the solution in the use process. Furthermore, the addition amount of the type I marine collagen in the acne-removing repair cream provided by the application is preferably 4% or 5% of the total components.

In addition, in the extraction process of the type I marine collagen provided by the application, a method for respectively treating sea cucumber bodies and sea cucumber viscera is adopted, and different parts of the sea cucumber can be treated and utilized more carefully, so that the extraction rate of the sea cucumber collagen is greatly improved, and the extraction rate of the collagen provided by the application can reach more than 30%, preferably more than 33%.

The components of the acne-removing cream of the present application and the extraction method of the marine collagen type I as the main active material are described in detail above, so that the acne-removing cream of the present application further comprises other components, which will be described in detail below, in order to better promote the absorption of collagen and antibacterial and anti-inflammatory effects on the skin with acne.

In some embodiments of the application, the sea cucumber-derived marine collagen acne-removing repair cream of the application further comprises a humectant comprising the following components: glycerol, propylene glycol and 1, 2-hexanediol. The propylene glycol and the glycerol can lock water and preserve moisture and promote the penetration of cosmetics, and the 1, 2-hexanediol can play a certain antibacterial role on the basis of preserving moisture. The humectant used in the application is a mild humectant, and the humectant containing irritant components such as ethanol is not adopted, so that the moisture can be safely and mildly locked. In some embodiments, the mass ratio of the three components of glycerol, propylene glycol and 1, 2-hexanediol is preferably 30-60:20-30:2-5, and most preferably 50:25:4.

In some embodiments of the present application, the emollient comprises butylene glycol, isopropyl myristate, isohexadecane, coconut oil, butter fruit and mineral oil, wherein the isohexadecane and isopropyl myristate have good nourishing effect on skin, and the rest substances are naturally extracted emollients, which can further play a role in water locking and moisture retention, provide a protective barrier for skin, and have no irritation to skin. Preferably, the mass ratio of the materials including butanediol, isopropyl myristate, isohexadecane, coconut oil, butter and mineral oil is preferably 1-5:1-5:0.5-5, and most preferably 2:2:5:2:2:2.

In some embodiments of the invention, the sea collagen acne-removing repair cream from sea cucumbers further comprises skin conditioning agents, wherein the skin conditioning agents comprise moringa seed oil, mango seed oil and royal jelly, the skin conditioning agents in the acne-removing repair cream are all skin conditioning agents extracted from natural substances, the moringa seed oil and the mango seed oil contain a large amount of phytosterol oleate, the skin conditioning agents can be used as conditioning agents and softening agents, the skin conditioning agents have a certain sun-proof effect, and the royal jelly has natural effects of resisting bacteria, diminishing inflammation, whitening, removing acnes and the like, and can eliminate inflammation.

Further, in some embodiments of the invention, the sea collagen acne-removing repair cream derived from sea cucumbers further comprises an antioxidant, wherein the antioxidant comprises natural vitamin E and astaxanthin oil, 60% of natural vitamin E, namely tocopherol, is adopted as the natural vitamin E, the effects of resisting aging and oxidization and supplementing nutrition can be achieved, 10% of astaxanthin oil is adopted as the astaxanthin oil, and the astaxanthin has the effects of resisting aging and oxidization and also has the effects of shrinking pores and cleaning free radicals.

In some embodiments of the present invention, in order to make the acne-removing cream of the present invention in emulsion form, and to make the use easier, a certain amount of emulsifier needs to be added, and thus, the emulsifier in the acne-removing cream of the present invention includes the following components: the viscosity of the repair cream can be adjusted to the most comfortable use viscosity by adding the emulsifier, wherein the emulsifier EG has a certain stabilizing effect, the addition amount of the emulsifier is lower, and the emulsification effect can be effectively achieved by matching multiple emulsifiers.

In order to improve the quality of the product, the emulsifying range of the emulsifying agent is enlarged to prevent the emulsion from phase separation during storage or transportation, and the emulsifying stabilizer is added, wherein the emulsifying stabilizer comprises cetyl alcohol/stearyl alcohol. The acne-removing repair cream added with the emulsion stabilizer not only can be stably stored, but also can play a certain role in lubrication, and improves the use comfort. In some embodiments, the mass ratio of the glyceryl monostearate, the tween 60, the tween 80, the span 60, the EG emulsifier and the steareth-21 is preferably 5-10:3-5:3-5:1-3:1-3, and most preferably 6:4:4:2:1:1.

Further, in order to prevent bacteria from entering the acne-removing repair cream due to improper storage of a user during use, a small amount of bacteriostatic agent is added into the acne-removing repair cream, and in some embodiments of the invention, the bacteriostatic agent comprises the following components: a water-soluble cool feeling agent and a plant bacteriostatic agent. Under the condition of playing a role in bacteriostasis, the water-soluble cold feeling agent can also provide a cool feeling and improve comfort, and the plant source bacteriostat can be a bacteriostasis component extracted from aloe, tea, pomegranate rind, mugwort or liquorice, in addition, the plant source bacteriostat can also be a bacteriostasis component extracted from natural Chinese herbal medicines, for example, honeysuckle, weeping forsythiae capsule, coptis chinensis, astragalus mongholicus, succus bambusae, common andrographis herb, selfheal fruit-spike, gallnut, scutellaria baicalensis, turmeric, perilla or mulberry, and the like, and the bacteriostat extracted from plants can be natural without irritation, has no harm to skin, and can achieve higher bacteriostasis effect. In some embodiments, the preservative of the present invention comprises phenoxyethanol, and in some preferred embodiments, the preservative of the present invention, phenoxyethanol, may be a compound phenoxyethanol (PH-CPH compound alcohol preservative available from Shanghai light industry research, all companies), i.e., a mixture of phenoxyethanol, 1, 2-hexanediol, chlorpheniramine, and water, in a mass ratio of 5:4:2.5:1, so that the concentration of phenoxyethanol can be further adjusted without irritation to the skin.

In some embodiments of the invention, the acne-removing repair cream is usually coated on the skin surface after the face is cleaned, so a certain amount of titanium dioxide is added as a sun-screening component, the titanium dioxide has a certain whitening effect, the titanium dioxide adopts hydrophilic ultra-fine titanium dioxide NTR-6 (available from Guangzhou Zhengqian biological technology Co., ltd./ZQ-080 BS aqueous titanium dioxide), the used sun-screening agent does not generate any burden on the human skin, and the use comfort can be improved.

In some embodiments of the invention, carbomer is added into the acne-removing repair cream as a thickener, wherein the carbomer can reduce dust powder in the production process, increase the dispersion rate, and can not cause the pH change of the system in the production process, and the viscosity of the finished product is easy to adjust. In order to adjust the product to the pH value range suitable for human skin, arginine is also added as a pH regulator, and arginine is used as the pH regulator to replace substances such as triethanolamine or sodium hydroxide in the traditional process, so that the system is milder, harmful components such as secondary alkylamine and nitrosamine are avoided, and cell proliferation and energy metabolism can be promoted. Arginine is taken as one of amino acids, can effectively play roles in whitening, moisturizing, softening skin, balancing grease and the like, can adjust the PH value of skin for a long time, and plays a role in long-acting oil control and inflammation inhibition.

In some embodiments of the application, the sodium hyaluronate in the acne removing repair cream disclosed by the application is small molecular sodium hyaluronate of 20-30 ten thousand Da, preferably small molecular sodium hyaluronate of 20-25 ten thousand Da, most preferably small molecular sodium hyaluronate of 23 ten thousand Da, and can play a role in moisturizing, provide nutrition for skin and promote the skin to absorb marine collagen.

In some embodiments of the present application, a small amount of essence and spice extracted from plants, such as aloe, rose, jasmine, gardenia, etc., is added to the acne-removing repair cream to improve the comfort level of use. In addition, the added EDTA-2Na as a chelating agent can reduce the influence of metal ions on the system and can adjust the pH value to a certain extent.

The water in the acne-removing repair cream disclosed by the application adopts deionized water as a matrix, so that any influence on various components is avoided, and the skin is not stimulated.

The beauty treatment cream provided by the application adopts the type I marine collagen, and can particularly excite the reconstruction of a collagen bracket at a scar (acne pit acne mark), so that the skin condition influenced by the scar is effectively repaired. The components such as the natural squalane, the antioxidant, the skin conditioner antibacterial agent and the like in the acne removing and repairing cream can play a role in diminishing inflammation and inhibiting bacteria, can play a role in removing acnes even if acnes caused by the inflammation of the sebaceous glands of hair follicles are eliminated, can inhibit tyrosinase from generating, prevents new pigmentation and has a long-term protection effect. Furthermore, the acne-removing repairing cream can be stored and transported at room temperature by adding the skin conditioning agent and the antibacterial agent, does not influence the acne-removing and long-acting repairing effects, and has higher stability. Further, various main components of the application, such as an emollient, a skin conditioner and the like, and various auxiliary components, such as an antioxidant, an antibacterial agent, a perfume essence and the like, are all components extracted from plants, so that the application has the advantages of good mildness, no irritation to skin and small use burden.

According to a second aspect of the invention, the invention also provides an acne-removing repair cream, which comprises the following components in percentage by mass: 1% -6% of marine collagen, 1% -5% of glycerol, 1% -5% of propylene glycol, 0.1% -1% of 1, 2-hexanediol, 1% -5% of butylene glycol, 1% -5% of isopropyl myristate, 1% -5% of isohexadecane, 0.5% -5% of coconut oil, 0.5% -5% of shea butter, 0.5% -5% of mineral oil, 1% -5% of glyceryl monostearate, 1% -5% of tween 60, 0.1% -1% of tween 80, 1% -5% of span 60, 0.1% -1% of EG emulsifier, 0.1% -1% of stearyl alcohol polyether-21, 0.5% -5% of cetyl alcohol/stearyl alcohol mixture, 0.1% -0.5% of titanium dioxide, 0.5% -5% of natural alkane, 0.1% -0.5% of sodium hyaluronate, 0.5% -5% of peppery seed oil, 0.5% -5% of mango seed oil, 0.5% -5% of royal jelly, 0.5% of vitamin E, 0.05% -0.05% of green oil, 0.05% of marine oil, and 0.01% -1% of vegetable-type marine collagen, wherein:

s2, performing secondary refining on the type I marine collagen macromolecule stock solution extracted in the step S1 to obtain type I marine collagen. The preparation method of the type I marine collagen is described in the above description, and will not be described herein.

Hereinafter, the present invention will be described in more detail by way of examples. These examples are only for illustrating the present invention and thus the present invention should not be construed as being limited to these examples.

Example 1

Extraction of type I marine collagen:

s1, extracting collagen from sea cucumbers to obtain type I marine collagen macromolecule stock solution:

s1-1, soaking the sea cucumber until no hard core exists, cutting open the belly of the sea cucumber, removing sand nozzles, peeling viscera of the sea cucumber from sea cucumber bodies, and cleaning the viscera and the bodies of the sea cucumber respectively;

s1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the sea cucumber body into an aqueous solution of acetic acid and xylitol with a molar ratio (molar ratio of acetic acid to xylitol) of 1:1 for soaking, adding pepsin for reaction for 10 hours, extracting at 4 ℃ for 50 hours, adjusting the pH value to 7.0, centrifuging to remove sediment, adding ethanol sediment polysaccharide into supernatant, and collecting supernatant, namely sea cucumber body collagen liquid;

S1-3, crushing the cleaned viscera of the sea cucumber, adding a first compound protease, adjusting the pH value to 8.0, reacting for 3 hours, adjusting the pH value of the enzymolysis solution to 7.0, and centrifugally separating to obtain a supernatant fluid, thus obtaining the viscera collagen solution of the sea cucumber;

s1-5, adding sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.0, then adding NaCl solution with the concentration of 5.1mol/L until the concentration of NaCl is 1.7mol/L, stirring, standing for 12 hours, centrifuging the solution, adding 4.0mol/L NaCl solution into the supernatant until the concentration of NaCl is 2.5mol/L, stirring, standing for 12 hours, centrifuging again, dissolving the precipitate in 1% acetic acid solution, dialyzing 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 10 times, and finally obtaining the type I marine collagen macromolecule stock solution;

s2, secondary refining of marine collagen

Uniformly mixing the type I marine collagen macromolecule stock solution and a sodium acetate buffer solution according to the proportion of 1:3, regulating the pH value to 8.0, adding a second compound protease, reacting for 4 hours at 40 ℃, inactivating the second compound protease by boiling water bath, centrifuging the mixed solution, taking supernatant, concentrating the supernatant, and obtaining the type I marine collagen after concentration.

Example 2

Extraction of type I marine collagen:

s1-1, soaking the sea cucumber until no hard core exists, cutting the sea cucumber into the sea cucumber belly, removing the sand mouth, peeling the viscera of the sea cucumber from the sea cucumber body, and cleaning the viscera and the sea cucumber body of the sea cucumber respectively;

s1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the sea cucumber body into an aqueous solution of acetic acid and xylitol with a molar ratio (molar ratio of acetic acid to xylitol) of 1:1 for soaking, adding pepsin for reaction for 8 hours, extracting at 4 ℃ for 50 hours, adjusting the pH value to 7.0, centrifuging to remove sediment, adding ethanol sediment polysaccharide into supernatant, and collecting supernatant, namely sea cucumber body collagen liquid;

s1-3, crushing the cleaned viscera of the sea cucumber, adding a first compound protease, adjusting the pH value to 8.0, reacting for 1h, adjusting the pH value of an enzymolysis solution to 7.0, and centrifugally separating to obtain a supernatant fluid, thus obtaining the viscera collagen solution of the sea cucumber;

s1-5, adding sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.0, then adding NaCl solution with the concentration of 5.1mol/L until the concentration of NaCl is 1.7mol/L, stirring, standing for 12 hours, centrifuging the solution, adding 4.0mol/L NaCl solution into the supernatant until the concentration of NaCl is 2.5mol/L, stirring, standing for 12 hours, centrifuging again, dissolving the precipitate in 1% acetic acid solution, dialyzing 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 10 times, and finally obtaining the type I marine collagen macromolecule stock solution.

S2, secondary refining of marine collagen:

s2-1, uniformly mixing the type I marine collagen macromolecule stock solution and a sodium acetate buffer solution according to a ratio of 1:3, regulating the pH value to 7.5, adding a second compound protease, reacting for 4 hours at 40 ℃, inactivating the second compound protease by boiling water bath, centrifuging the mixed solution, taking supernatant, concentrating the supernatant, and obtaining the type I marine collagen.

Example 3

Extraction of type I marine collagen:

s1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the sea cucumber body into an aqueous solution of acetic acid and xylitol with a molar ratio (molar ratio of acetic acid to xylitol) of 1:1 for soaking, adding pepsin for reaction for 10 hours, extracting at 4 ℃ for 50 hours, adjusting the pH value to 7.5, centrifuging to remove sediment, adding ethanol sediment polysaccharide into supernatant, and collecting supernatant, namely sea cucumber body collagen liquid;

S1-3, crushing the viscera of the sea cucumber cleaned in the step S1-1, adding a first compound protease, adjusting the pH value to 8.5, reacting for 3 hours, adjusting the pH value of the enzymolysis solution to 7.5, and centrifugally separating to obtain supernatant fluid, thus obtaining the viscera collagen solution of the sea cucumber;

s1-5, adding sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.5, then adding NaCl solution with the concentration of 5.1mol/L until the concentration of NaCl is 1.7mol/L, stirring, standing for 18h, centrifuging the solution, adding 4.0mol/L NaCl solution into the supernatant until the concentration of NaCl is 2.5mol/L, stirring, standing for 18h, centrifuging again, dissolving the precipitate in 1% acetic acid solution, dialyzing the 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 12 times, and finally obtaining the type I marine collagen macromolecule stock solution.

S2, secondary refining of marine collagen:

Example 4

Extraction of type I marine collagen:

s1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the sea cucumber body into an aqueous solution of acetic acid and xylitol with a molar ratio (molar ratio of acetic acid to xylitol) of 1:1 for soaking, adding pepsin for reaction for 9 hours, extracting at 4 ℃ for 50 hours, adjusting the pH value to 7.5, centrifuging to remove sediment, adding ethanol sediment polysaccharide into supernatant, and collecting supernatant, namely sea cucumber body collagen liquid;

s1-3, crushing the viscera of the sea cucumber cleaned in the step S1-1, adding a first compound protease, adjusting the pH value to 9.0, reacting for 2 hours, adjusting the pH value of the enzymolysis solution to 7.5, and centrifugally separating to obtain supernatant fluid, thus obtaining the viscera collagen solution of the sea cucumber;

s1-5, adding sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.5, then adding NaCl solution with the concentration of 5.1mol/L until the concentration of NaCl is 1.7mol/L, stirring, standing for 24 hours, centrifuging the solution, adding 4.0mol/L NaCl solution into the supernatant until the concentration of NaCl is 2.5mol/L, stirring, standing for 24 hours, centrifuging again, dissolving the precipitate in 1% acetic acid solution, dialyzing the 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 11 times, and finally obtaining the type I marine collagen macromolecule stock solution.

S2, secondary refining of marine collagen:

s2-1, uniformly mixing the type I marine collagen macromolecule stock solution and a sodium acetate buffer solution according to a ratio of 1:3, regulating the pH value to 8.0, adding a second compound protease, reacting for 4 hours at 40 ℃, inactivating the second compound protease by boiling water bath, centrifuging the mixed solution, taking supernatant, concentrating the supernatant, and obtaining the type I marine collagen.

The determination of the molecular weight and the extraction rate of the marine collagen prepared in examples 1 to 4 will be described below.

Test example 1

Molecular weight determination of marine collagen

The molecular weight of collagen was determined according to the industry executive standard QBT 2732-2005-hydrolyzed collagen, using gel chromatography, as shown in table 1 below:

table 1 molecular weight of marine collagen in examples 1 to 4

Examples	Example 1	Example 2	Example 3	Example 4
					Molecular weight	1200Da-120kDa	1700Da-150kDa	1000Da-120kDa	1200Da-150kDa

The molecular weight of the type I marine collagen prepared by the preparation method of the marine collagen provided by the invention is 1000Da-150kDa, which is favorable for skin absorption and utilization.

Test example 2

Determination of the extraction yield of marine collagen

The extraction rate of marine collagen refers to the ratio of the mass of a target substance (marine collagen) successfully extracted from a raw material to the total mass of the target substance in the raw material, and thus the calculation of the extraction rate of collagen in sea cucumber can be performed as follows:

wherein the method comprises the steps ofI.e. the mass of marine collagen extracted according to the method of the invention, < > i->Namely, the quality of collagen originally existing in the sea cucumber is calculated by measuring the content of hydroxyproline in the extracting solution. Specifically, hydroxyproline content determination passes national standards: the method in GB/T9695.23-2008 meat and meat products hydroxyproline content determination is calculated, 3mL of collagen solution in each embodiment is taken, 3mL of 6mol/L hydrochloric acid is added, digestion is carried out for 45min by a MARS microwave instrument (power 800W, temperature 180 ℃) and the hydroxyproline content is determined by a hydrolysate, the hydroxyproline content is multiplied by a conversion coefficient of 14.7, and the collagen content is calculated:

wherein, the liquid crystal display device comprises a liquid crystal display device,the content of hydroxyproline in the sample is percent; />Concentration of hydroxyproline in sample solution, μg/mL, obtained from standard curve; / >Sucking the volume of the filtrate, and mL; />G is the mass of the sample. Thus, the obtained hydroxyproline content was multiplied by a conversion factor of 14.7 to obtain a collagen content.

Type I marine collagen was extracted according to the methods of examples 1 to 4 above, the extracted type I marine collagen was dried under nitrogen atmosphere, and the mass of the dried type I marine collagen was recorded, which was counted as. Thus, the extraction rate of the type I marine collagen in each example can be calculated.

The calculated extraction rates of type I marine collagen of the above examples 1 to 4 are shown in Table 2 below

TABLE 2 extraction yield of sea collagen from examples 1 to 4

Examples	Example 1	Example 2	Example 3	Example 4
					Molecular weight	31.2%	33.7%	34.5%	33.9%

From the results, the extraction rate of the type I marine collagen prepared by the method is up to more than 30%, more preferably, can reach more than 33.5%, and the method has high extraction rate, high efficiency and great economic benefit.

The following provides examples of the sea cucumber-derived marine collagen acne-removing cream of the present invention, so as to better illustrate the regulation of the proportion of each component in the acne-removing cream of the present invention. The components in the examples below were purchased from Guangzhou Zhengli chemical Co., ltd unless specified. In addition, the component grades in the following examples are all cosmetic grades.

Example 5

The sea collagen acne-removing repair cream comprises the following components in percentage by mass: 6% marine collagen, 5% glycerol, 2.5% propylene glycol (available from Guangzhou Shi Ganyun Co., ltd.), 0.4% 1, 2% hexanediol, 1% butylene glycol, 2% isopropyl myristate, 4% isohexadecane (Japanese light), 2% coconut oil, 0.5% shea butter, 0.5% mineral oil, 3% glyceryl monostearate, 60% Tween 1%, 0.1% Tween 80, 60% span 60%, EG emulsifier (available from Guangzhou Chemie Co., ltd.) 0.5%, steareth-21.1% cetyl alcohol/stearyl alcohol mixture 1%, titanium dioxide (available from Guangzhou Long Xin New Material technologies Co., ltd.) 0.2%, natural squalane 2%, sodium hyaluronate 0.1%, moringa seed oil (available from Gibber's Hill) 0.5%, royal jelly 0.5%, natural vitamin E1%, green oil 0.1%, carbomer (available from Gibber's chemical industry Co., ltd.) 0.62%, 0.01% water soluble in water, 0.0.01% water, 0.2% water soluble in film, 0.0% water, 0.01% water soluble in film, etc., wherein the marine collagen is type I marine collagen extracted from sea cucumber.

Example 6

The sea collagen acne-removing repair cream comprises the following components in percentage by mass: the sea collagen acne-removing repair cream comprises the following components in percentage by mass: marine collagen 4%, glycerol 3%, propylene glycol 2.5%, 1, 2-hexanediol 1%, butylene glycol 4%, isopropyl myristate 1%, isohexadecane 2%, coconut oil 1%, shea butter 1%, mineral oil 1%, glyceryl monostearate 3%, tween 60%, tween 80.5%, span 60%, EG emulsifier 0.5%, steareth-21.5%, cetyl alcohol/stearyl alcohol mixture 0.5%, titanium dioxide 0.2%, natural squalane 3%, sodium hyaluronate 0.2%, moringa seed oil 2%, mango seed oil 2%, royal jelly 1%, natural vitamin E2%, astaxanthin oil 0.2%, carbomer 0.5%, arginine 0.45%, phenoxyethanol 0.1%, water-soluble cooling agent 0.03%, plant-derived marine bacteriostat 0.05%, essence and perfume 0.05%, EDTA-2na 0.05% and the balance water (56.67% water), wherein collagen is type I collagen extracted from sea cucumber.

Example 7

The sea collagen acne-removing repair cream comprises the following components in percentage by mass: marine collagen 3%, glycerol 1%, propylene glycol 1%, 1, 2-hexanediol 0.2%, butylene glycol 1%, isopropyl myristate 1%, isohexadecane 1%, coconut oil 2%, shea butter 1%, mineral oil 1%, glyceryl monostearate 1%, tween 60 1%, tween 80.1%, span 60%, EG emulsifier 0.1%, steareth-21.1%, cetyl alcohol/stearyl alcohol mixture 0.5%, titanium dioxide 0.1%, natural squalane 0.5%, sodium hyaluronate 0.1%, moringa seed oil 0.8%, mango seed oil 1%, royal jelly 0.5%, natural vitamin E0.5%, astaxanthin oil 0.1%, carbomer 0.05%, arginine 0.1%, phenoxyethanol 0.1%, water-soluble marine cooling agent 0.01%, botanical antibacterial agent 0.03%, sea cucumber flavor 0.03%, EDTA-2na 0.08% and the balance water (80.00% water), wherein marine collagen is type I collagen extracted from sea cucumber.

Example 8

A sea cucumber-derived marine collagen acne-removing repair cream comprises, by mass, 6% of marine collagen, 2% of glycerol, 5% of propylene glycol, 1, 2-hexanediol, 1% of butanediol, 1% of isopropyl myristate, 2% of isohexadecane, 2% of coconut oil, 2% of shea butter, 2% of mineral oil, 5% of glyceryl monostearate, 60% of tween, 0.1% of tween 80, 60% of span, 0.1% of EG emulsifier, 0.1% of steareth-21, 5% of cetyl alcohol/stearyl alcohol mixture, 0.5% of titanium dioxide, 5% of natural squalane, 0.5% of sodium hyaluronate, 4.6% of moringa seed oil, 4.3% of mango seed oil, 2% of royal jelly, 4.3% of natural vitamin E, 0.5% of astaxanthin oil, 1% of carbomer, 0.5% of arginine, 0.25% of phenoxyethanol, 0.05% of water-soluble sensitizer, 0.01% of plant-derived antibacterial agent, 0.01% of EDTA-2Na 0.1% of perfume and the balance water (36.04%), wherein the sea cucumber is collagen I extracted from sea cucumber.

Example 9

The sea collagen acne-removing repair cream comprises the following components in percentage by mass: 2% marine collagen, 3% glycerol, 3% propylene glycol, 1, 2-hexanediol, 2% butylene glycol, 2% isopropyl myristate, 3% isohexadecane, 2% coconut oil, 1% shea butter, 1% mineral oil, 2.5% glyceryl monostearate, 60.5% tween 80.1%, 60% span, 0.7% EG emulsifier, 0.3% steareth-21, 2% cetyl/stearyl alcohol mixture, 0.25% titanium dioxide, 2% natural squalane, 0.2% sodium hyaluronate, 0.8% moringa seed oil, 0.8% mango seed oil, 4% royal jelly, 3% natural vitamin E, 0.1% astaxanthin oil, 0.5% carbomer, 0.1% arginine, 0.1% phenoxyethanol, 0.02% water soluble cooling agent, 0.03% botanical antibacterial agent, 0.01% essence and 0.07% EDTA-2na and the balance water (57.92% water), wherein marine collagen is sea cucumber type I collagen extracted from the marine collagen.

Examples 5-9 above provided marine collagen acne-removing cream of different composition contents from sea cucumber sources, and type I marine collagen used in examples 5-9 above was extracted by the method of example 1. The above examples are illustrative, but not limiting, and the microbial content, physicochemical index, efficacy and effect on skin and acute skin irritation of the anti-acne cream provided by the present invention are tested by taking the above example 5 as an example.

Test example 3

Microbial detection of sea collagen acne-removing repair cream from sea cucumber source

Taking the acne-removing repair cream of the embodiment 5 of the invention as an example, the indexes of the total colony count, the total mould and saccharomycete count, the heat-resistant coliform group, staphylococcus aureus and pseudomonas aeruginosa are detected. Detection method and index are compounded with the requirement of cosmetic safety technical Specification (2015 edition) on microorganism indexes. The results of the microbiological detection are shown in Table 3:

TABLE 3 microbial test results

As can be seen from the results in Table 3, three pathogenic bacteria which are easy to appear in cosmetics, namely heat-resistant coliform bacteria, staphylococcus aureus and pseudomonas aeruginosa, are not detected in the acne-removing repair cream provided by the invention, and the total number of bacterial colonies, mould and saccharomycetes are smaller than the limit value, so that the safety of the acne-removing repair cream provided by the invention can be proved to be extremely high.

Test example 4

Physicochemical inspection of sea collagen acne-removing repair cream from sea cucumber source

Taking the acne-removing repair cream of the embodiment 5 of the invention as an example for detecting the content of mercury, lead, arsenic, cadmium and dioxane, the detection method and the index are both in accordance with the requirements of cosmetic safety technical Specification (2015 edition). The results are shown in Table 4:

TABLE 4 physicochemical test results

The results in table 4 show that the detection concentrations of the above five components of mercury, lead, arsenic, cadmium and dioxane are lower than the limit value, and the fact that the anti-acne repair cream provided by the invention has extremely low toxic and harmful substances and does not affect the human body can be proved.

Test example 5

Efficacy of sea collagen acne-removing repair cream from sea cucumber source and influence detection on skin

The detection method adopts T/TDCA 004-2021 (front and back comparison of the method) for testing the acne removing efficacy of cosmetics, adopts Guangdong society of cosmetics standard T/GDCA 009-2022 (human evaluation method for the repairing efficacy of cosmetics) for testing the efficacy of cosmetics, and 30 cases of subjects 18-40 years old participate in the whole course of the test, and the number of red acne spots, the total area of red areas and the percutaneous moisture loss rate of the cheeks of the subjects before and after the acne removing repairing cream is used are observed in an instrument test mode after the subjects use the acne removing repairing cream for 7 days, so that the acne removing and repairing efficacy of the acne removing repairing cream is evaluated, the acne removing and repairing effects of the acne removing repairing cream are kept dry during the test, and the acne removing and repairing effects of the acne removing repairing cream are not applicable to any liquid substances, other cosmetics, medicaments and health care products which have influence on the results. In addition, after the whole test is completed, the subject will complete a questionnaire, and the knowledge of the acne-removing repair cream of the present invention, the opinion of the product efficacy, and the attitude of the subject to the acne-removing repair cream of the present invention are expressed by answering the questionnaire.

Table 5 below lists the items, methods, instrumentation, test sites and detection principles tested.

Table 5 evaluate item content list

The test schedule is shown in table 6 below:

TABLE 6 test schedule

Note that: "good" indicates that the implementation was performed and "-" indicates that the implementation was not performed.

The SPSS software is used for carrying out descriptive statistics on each measured value, including the number, the mean value and the standard deviation. Comparison of front and rear: performing significance Test on normal distribution of data improvement values by using Shapiro-Wilk Test, wherein Sig (double sides) >0.01 is in normal distribution; t-test was performed with a significant difference level α of 0.01. If sig (double sided) <0.01, a non-normal distribution is performed, rank sum test is performed, and the significance difference level α is 0.05. Table 7 below shows the number of red spot spots and the average total area of the spots.

TABLE 7 descriptive statistics of number of red spot and total area of spot

/>

"ns" means that p > 0.05, ". Times.0.01.ltoreq.p < 0.05; "" means that 0.001. Ltoreq.p <0.01, "" means that p < 0.001.

Fig. 1 shows the number of red spot and total spot area test values of the acne-removing repair cream before use and on the 14 th day, and a comparison graph, and it can be seen that after the product is used for 14 days, compared with a D0 average value, skin test related parameters show that: the number of acne points of a subject is reduced by 24.61%, the saliency factor p is less than 0.001, the area of the acne points is reduced by 43.15%, and the saliency factor p is less than 0.001, so that the acne-removing repair cream disclosed by the invention has an obvious acne-removing effect.

Table 8 below shows the percutaneous moisture loss test on day 14 and when not in use with the anti-acne cream of the present invention:

table 8 descriptive statistics of percutaneous moisture loss test values

"ns" means that p > 0.05, ". Times.0.01.ltoreq.p < 0.05; "" means that 0.001. Ltoreq.p < 0.01, "" means that p < 0.001.

Fig. 2 shows a comparison of the percutaneous moisture lapse test values of the anti-acne cream according to the present invention, which were tested before and at the 14 th day of use. According to the results, the acne-removing repair cream provided by the invention has the advantages that the percutaneous moisture loss rate is reduced by 23.48% on the 14 th day compared with the average value before use, and the significance factor p is less than 0.001, so that the acne-removing repair cream provided by the invention has obvious repair efficacy, can prevent moisture loss and plays a role of long-term barrier.

Table 9 below shows the satisfaction of subjects with the anti-acne treatment cream of the present invention as indicated by a questionnaire.

Table 9 subject evaluation satisfaction

Sequence number	Problem(s)	Improvement (satisfaction) rate (%) after 14 days of use
			1	When in use, the acne-removing repair cream is mild and does not irritate	100%
2	After the acne-removing repairing cream is used, the acne-removing repairing cream improves redness caused by skin damage	100%
			3	After the acne-removing repair cream is used, the acne-removing repair cream has long-acting moisturizing effect	96.7%
4	After use, the acne-removing repair cream improves skin dryness	96.7%
			5	After the skin lotion is used, the skin moisture degree is improved	100%
6	After use, acne removing and repairingCream calm and relieve skin	100%
			7	After the use, the acne-removing repair cream is felt to moisten skin	96.7%
8	After the acne-removing repairing cream is used, the acne-removing repairing cream is considered to repair skin barrier	96.7%
			9	When in use, the acne-removing and repairing cream has good skin feel and is not greasy	93.3%
10	After the use, the acne-removing repairing cream is considered to reduce grease secretion	96.7%
			11	After the use, the acne-removing repairing cream is felt to have the oil control effect	96.7%
12	After the acne-removing repairing cream is used, the acne-removing repairing cream is perceived to improve the redness of acne parts	100%
			13	After the acne-removing repairing cream is used, the skin is not tight after the acne-removing repairing cream is used	96.7%
14	After the acne-removing repairing cream is used, the acne-removing repairing cream is not long in fat particles	100%
			15	Overall, the anti-acne repair cream is satisfactory	100%

Note that: and (3) statistics: satisfaction = number of significant persons scored > 3/(total significant persons x 100%)

As can be seen from table 9, in the subjects in the test, after 14 days of using the acne-removing repair cream of the present invention, 100.0% of the subjects feel the acne-removing repair cream of the present invention mild and non-irritating, and the redness caused by skin loss can be improved, the skin moisture degree can be improved, the skin can be calmed and relaxed, and the acne partial redness and the non-growing fat particles can be improved; 96.7% of subjects feel that the acne-removing repair cream has a long-acting moisturizing effect, can improve dry skin, moisten skin, repair skin barrier, reduce grease secretion, feel oil control effect and make skin not tight; 93.3% of subjects feel that the acne-removing repair cream of the invention has good skin feel and is not greasy. 100.0% of the subjects are satisfied with the skin care effect of the product.

In conclusion, the effects of the acne removing repair cream and the effects of the acne removing repair cream on the skin show that after 30 subjects use the acne removing repair cream, compared with a D0 average value, the number of red acne points and the total area of the acne points are obviously reduced (p is less than 0.001), and the acne removing repair cream has an acne removing effect; after the acne-removing repair cream provided by the invention is used for 14 days, compared with the average value of D0, the percutaneous moisture loss rate is remarkably reduced (p is less than 0.001), which proves that the acne-removing repair cream provided by the invention has a repair effect; and the satisfaction degree of the subjects on the acne-removing repair cream is extremely high.

Test example 6

Acute skin irritation test

The acne-removing cream of the present invention (example 5) was tested for acute skin irritation using animal experiments, in which the acne-removing cream of the present invention was used as a test substance. The experimental animals were New Zealand rabbits (common grade, male, weight 2.5 kg), animal eligibility number: no.430730221100242674; the test animal production license number was purchased from the biological technology company, taiping, hunan: SCXK (Xiang) 2020-0005; feeding environment: ordinary environment, temperature 24.0-24.5 ℃, relative humidity 65.9-68.7RH%, experimental animal environment use license number: SYXK (Guangdong) 2017-0216; the experimental animal feed is provided by Jiangsu province cooperative medical bioengineering Limited liability company, license number: su Sizheng (2019) 01008, quality qualifier: no. qcc20221000074401.

According to the test method, adult healthy skin intact rabbits are selected, and the fur on the two sides of the spine of the back of the rabbit is reduced 24 hours before the test, so that the epidermis cannot be damaged, and the fur removing range is 3cm multiplied by 3cm on the left and right sides. In the test, 0.5g of the test substance was applied to the left skin, and then covered with two layers of gauze (2.5 cm. Times.2.5 cm) and one layer of cellophane, and fixed with a non-irritating adhesive tape and bandage. The right skin was coated with an equal amount of distilled water as a control, and the residual test object was removed with warm water after the test was completed. Skin reactions at the application site were observed 1h, 24h, 48h and 72h after removal of the test substance, and skin irritation reactions were recorded. The results are shown in Table 10 below.

Table 10 test results of acute skin irritation test on Rabbit

Note that: the integrated mean retains 2-bit decimal.

Fig. 3 shows a test picture of acute skin irritation of a test object to rabbits, and as can be seen from table 10 and fig. 3, the highest integral mean value of the test object (namely, the acne removing repair cream of the invention) on the acute skin irritation of the rabbits is 0.00, and the irritation intensity is non-irritation.

In conclusion, according to the acne-removing repair cream disclosed by the invention, I-type marine collagen is added to be combined with other components, so that acne removal and repair are combined, amino acids required for sufficient wound healing are provided in the acne removal and repair processes, the new generation and rearrangement of collagen fibers and elastic fibers in the dermis layer can be promoted in repair, the existing inflammatory pigmentation can be dissipated, tyrosinase is inhibited, new pigmentation is prevented, effective absorption of skin is promoted, and the acne-removing repair cream has efficacy and no irritation; the acne-removing repair cream disclosed by the invention has good stability, and can be stored and transported at room temperature without affecting the acne-removing and long-acting repair effects. The acne-removing repair cream has the double functions of acne removal and repair, screens 1400 sea cucumbers in a pollution-free sea area, determines the variety of the sea cucumbers which are not edible and nontoxic in the pollution-free sea area, extracts the collagen of the sea cucumbers, performs secondary development and fine processing, improves the sampling safety and the collagen extraction efficiency, and has extremely high market application value.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to be exhaustive or to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and their practical application to enable others skilled in the art to make or utilize the invention in various exemplary embodiments and with various alternatives and modifications. It is intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

It is to be understood that the above embodiments are merely illustrative of the application of the principles of the present invention, but not in limitation thereof. Various modifications and improvements may be made by those skilled in the art without departing from the spirit and substance of the invention, and are also considered to be within the scope of the invention. In the description of the present specification, the descriptions of the terms "one embodiment," "some embodiments," "examples," "particular examples," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Claims

1. The sea cucumber-derived marine collagen acne-removing repair cream is characterized by comprising the following components in percentage by mass: 1% -6% of marine collagen, 5% -10% of humectant, 5% -10% of emollient, 5% -10% of emulsifier, 0.5% -5% of emulsion stabilizer, 0.1% -0.5% of titanium dioxide, 0.5% -5% of natural squalane, 0.1% -0.5% of sodium hyaluronate, 1% -15% of skin conditioner, 0.1% -5% of antioxidant, 0.05% -1% of thickener, 0.1% -0.5% of pH regulator, 0.1% -1% of preservative, 0.01% -0.1% of antibacterial agent, 0.01% -0.05% of essence and spice, 0.05% -0.1% of EDTA-2Na and the balance of water, wherein the marine collagen is I-type marine collagen extracted from sea cucumbers, and the I-type marine collagen is prepared by the following method:

s2, performing secondary refining on the type I marine collagen macromolecular stock solution extracted in the step S1 to obtain type I marine collagen, wherein

The step S1 includes:

s1-1, soaking sea cucumber until no hard core exists, cutting open the belly of the sea cucumber, removing sand nozzles, peeling off viscera and bodies of the sea cucumber, and cleaning the viscera and bodies of the sea cucumber respectively;

S1-2, crushing the sea cucumber body cleaned in the step S1-1, adding the crushed sea cucumber body into an aqueous solution of acetic acid and xylitol, soaking the sea cucumber body, adding pepsin for reaction for 8-10 hours, extracting the sea cucumber body at the temperature of 4 ℃ for 50-h, adjusting the pH value to 7.0-7.5, centrifuging the sea cucumber body to remove sediment, adding ethanol sediment polysaccharide into supernatant, and collecting the supernatant to obtain sea cucumber body collagen liquid;

s1-3, crushing the sea cucumber viscera washed in the step S1-1, adding a first compound protease, adjusting the pH value to 8.0-9.0, reacting for 1-3 hours, adjusting the pH value of the enzymolysis solution to 7.0-7.5, and centrifugally separating to obtain a supernatant fluid, thus obtaining the sea cucumber viscera collagen solution;

s1-5, adding sodium acetate buffer solution into the collagen mixed solution, regulating the pH value to 7.0-7.5, then adding NaCl solution with the concentration of 5.1 mol/L until the concentration of NaCl is 1.7 mol/L, standing for 10-24 hours after stirring, centrifuging the solution, adding 4.0 mol/L NaCl solution into the supernatant until the concentration of NaCl is 2.5 mol/L, standing for 10-24 hours after stirring, centrifuging again, dissolving precipitate in 1% acetic acid solution, dialyzing 1% acetic acid, repeatedly dissolving the precipitate and dialyzing for 10-12 times to finally obtain the type I marine collagen macromolecular stock solution, and

The step S2 includes:

s2-1, uniformly mixing the type I marine collagen macromolecule stock solution and a sodium acetate buffer solution according to a ratio of 1:3, regulating the pH value to 7.5-8.5, adding a second compound protease, reacting at 40 ℃ for 4 h, inactivating the second compound protease by boiling water bath, centrifuging the mixed solution, taking the supernatant, concentrating the supernatant, and obtaining the type I marine collagen, wherein the type I marine collagen is prepared by the steps of

In the step S1-2, the molar ratio of the acetic acid to the xylitol in the aqueous solution of the acetic acid and the xylitol is 1:1;

the first complex protease includes papain, pepsin, and Flavourzyme protease; the second complex protease comprises alkaline protease and trypsin;

the extraction rate of the type I marine collagen is 31.2% -34.5%.

2. The sea cucumber-derived marine collagen acne-removing repair cream of claim 1, wherein the moisturizer comprises the following components: glycerol, propylene glycol and 1, 2-hexanediol; the emollient includes butylene glycol, isopropyl myristate, isohexadecane, coconut oil, butter fruit and mineral oil.

3. The sea cucumber-derived marine collagen acne-removing repair cream of claim 1, wherein the skin conditioner comprises moringa seed oil, mango seed oil and royal jelly.

4. Sea cucumber-derived marine collagen acne-removing repair cream according to claim 1, wherein the antioxidant comprises natural vitamin E and astaxanthin oil.

5. The sea cucumber-derived marine collagen acne-removing repair cream of claim 1, wherein the emulsifier comprises the following components: glyceryl monostearate, tween 60, tween 80, span 60, EG emulsifier and stearyl alcohol polyether-21; the emulsion stabilizer comprises cetyl alcohol/stearyl alcohol.

6. The sea cucumber-derived marine collagen acne-removing repair cream of claim 1, wherein the antibacterial agent comprises the following components: the water-soluble cool sensing agent and the plant-source bacteriostatic agent, wherein the preservative comprises phenoxyethanol.

7. Sea cucumber-derived marine collagen anti-acne treatment cream according to claim 1, wherein the thickener comprises carbomers; the pH adjuster comprises arginine.

8. The sea cucumber-derived marine collagen acne-removing repair cream is characterized by comprising the following components in percentage by mass: 1% -6% of marine collagen, 1% -5% of glycerol, 1% -5% of propylene glycol, 0.1% -1% of 1, 2-hexanediol, 1% -5% of butylene glycol, 1% -5% of isopropyl myristate, 1% -5% of isohexadecane, 0.5% -5% of coconut oil, 0.5% -5% of shea butter, 0.5% -5% of mineral oil, 1% -5% of glyceryl monostearate, 1% -5% of tween 60, 0.1% -1% of tween 80, 1% -5% of span 60, 0.1% -1% of EG emulsifier, 0.1% -1% of stearyl alcohol polyether-21, 0.5% -5% of cetyl alcohol/stearyl alcohol mixture, 0.1% -0.5% of sodium hyaluronate, 0.5% -5% of moringa seed oil, 0.5% -5% of mango seed oil, 0.5% -5% of royal jelly, 0.5% of vitamin E, 0.5% -5% of green oil, 0.05% of marine oil, 0.01% -1% of marine collagen, and 0.01% -1% of vegetable oil, wherein:

The step S1 includes:

the step S2 includes:

The extraction rate of the type I marine collagen is 31.2% -34.5%.