JP2006151847A - Collagen peptide composition, method for producing the same and cosmetic composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a collagen peptide composition that dissolves the problems that the conventional collagen has and can be used in skin cosmetics or agents without damage to the originally excellent functions of the collagen peptide and can give excellent sense of use, when it is mixed in cosmetics or medicines. <P>SOLUTION: This is a collagen composition obtained by hydrolysis of collagen, and comprises 60 to 100 wt.% of peptides having a molecular weight of from 400 to less than 3,000, less than 25 wt.% of peptides of a molecular weight of less than 400 and less than 15 wt.% of peptides of a molecular weight of more than 3,000. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a collagen peptide composition, a method for producing the same, and a cosmetic composition. Specifically, the present invention relates to a peptide composition obtained by degrading collagen contained in connective tissues of animals, and such a collagen peptide composition. And a cosmetic composition using a collagen peptide composition.

It is already known that collagen peptides are effective as components for skin cosmetics and pharmaceuticals. It is also added to foods such as beverages and used to improve health promotion functions.
Dermal cosmetics and dermatological drugs containing collagen peptides not only provide the skin surface with a moisturizing function by means of collagen peptides, but the collagen peptides can penetrate into the skin and improve the function of skin cells. It has been reported. It is also known that the molecular weight and molecular weight distribution of peptides affect the above functions.
As a technique for producing a collagen peptide, a general technique for extracting collagen from animal raw materials and a technique for degrading collagen are applied.

For example, Patent Document 1 discloses a peptide whose amino acid sequence is (Gly-XY) n: n = 1 to 3 having a molecular weight of 1000 or less and having no antigenicity, by specifically degrading a collagen raw material with a collagenase enzyme. Techniques for producing the majority of collagen peptides have been shown. The obtained collagen peptide is said to be effective for prevention and treatment of food allergy, bone treatment, bone metabolism improvement, wound healing and the like.
Patent Document 2 discloses a technique for producing a collagen peptide containing a large amount of tripeptides and dipeptides by degrading a collagen component with a cysteine protease enzyme. The obtained collagen peptide is said to have a collagen production promoting action, an osteoblast proliferation promoting action, a bone strengthening action and a skin activating action.
Japanese Patent No. 3146251 JP 2004-244369 A

Conventionally, collagen peptides excellent in skin permeability suitable for skin cosmetics and pharmaceuticals have been considered to be tripeptides and dipeptides having a molecular weight of less than 300. For this reason, as a collagen peptide production technique, research and development have been carried out in order to find production conditions in which low molecular weight components such as the above-described tripeptides and dipeptides can be obtained as efficiently as possible.
However, cosmetics and pharmaceuticals for skin using conventional collagen peptides have a problem that the feeling of use when applied to the skin is not so good.
For example, as described in Patent Document 1, a solution mainly containing a tripeptide flows down or spreads immediately when applied to the skin, and it is difficult to sufficiently adhere to a predetermined site. It is. The feeling of extending smoothly along the skin is poor, and the feeling of use is not good.

When preparing cosmetics, it may be possible to add a viscosity modifier or thickener in addition to the collagen peptide to improve the stretchability and feel when used on the skin. Therefore, the target function is not sufficiently exhibited. A thickener or the like may inhibit the penetration function of collagen peptides into the skin.
The problem of the present invention is to solve the problems of the conventional collagen peptide described above, and to reduce the problem of use when blended in cosmetics and pharmaceuticals for skin without impairing the excellent functions inherent to the collagen peptide. Is to provide an excellent collagen peptide composition.

The collagen peptide composition according to the present invention is a composition of a collagen peptide which is a degradation product of collagen, wherein the peptide having a molecular weight of 400 or more and less than 3000 is 60 to 100% by weight, and the peptide having a molecular weight of less than 400 is 25% by weight. And a peptide having a molecular weight of 3000 or more and less than 15% by weight.
[Collagen peptide composition]
Basically, it is a degradation product of collagen and consists of a collection of substances included in the concept of peptides.
A peptide is a peptide bond of two or more amino acids. Those consisting of two amino acids are called dipeptides, and those consisting of three amino acids are called tripeptides. Moreover, what consists of 10 or more amino acids may be called a polypeptide, and what consists of less than 10 amino acids may be called an oligopeptide. The greater the number of amino acid bonds, the greater the molecular weight of the peptide. For example, the molecular weight of the tripeptide is about 300, and the molecular weight of the polypeptide is 1000 or more. However, the molecular weight may be different for the same tripeptide if the combination of amino acids is different.

Collagen peptides may be distinguished by differences in collagen-containing raw materials that have become the raw materials for extraction and decomposition. For example, it is called bovine bone-derived collagen peptide, scab-derived collagen peptide, or the like. Among them, fish scale-derived collagen peptides and animal Achilles tendon-derived collagen peptides with less oil and fat and impurities are preferred. Among the animal Achilles tendon-derived collagen peptides, avian Achilles tendon-derived collagen peptides or ostrich Achilles tendon-derived collagen peptides are preferred.
The peptide composition does not need to be composed of only a specific number of peptides with amino acid bonds, and includes various peptides regardless of the number and combination of amino acid bonds. The distribution conditions are satisfied.

The molecular weight distribution of the peptide composition can be measured by a conventional method. For example, the HPLC method (High Performance Liquid Chromatography) is employed.
The molecular weight distribution consists of 60 to 100% by weight of peptides having a molecular weight of 400 or more and less than 3000, less than 25% by weight of peptides having a molecular weight of less than 400, and less than 15% by weight of peptides having a molecular weight of 3000 or more. It is desirable that the peptide having a molecular weight of 400 or more and less than 1500 is 55 to 100% by weight. The peptide having a molecular weight of less than 400 is desirably less than 15% by weight, and more preferably less than 10% by weight. The peptide having a molecular weight of 3000 or more is preferably less than 10% by weight, and more preferably less than 5% by weight.

  When a peptide having a molecular weight of 400 or more and less than 3000 is contained in a sufficient ratio, it is possible to make the loading or elongation appropriate when a cosmetic or the like containing the peptide composition is applied to the skin. The functions of the peptide, such as the effect of improving skin permeability and cell function, are also practically sufficiently excellent. When there are too many peptides with a molecular weight of less than 400, the cosmetics containing the peptide composition have almost no moist and moisturizing feeling, and feel like a mere water. When there are too many peptides with a molecular weight of 3000 or more, the cosmetics containing the peptide composition give a greasy and unpleasant feel. The permeability to the skin and the effect of improving cell function also deteriorate.

With the molecular weight distribution as described above, the weight average molecular weight of the collagen peptide composition can be set to 600 to 1800. If the weight average molecular weight is too large, it will be difficult to stretch when applied to the skin. The skin penetration function and the like are also reduced. If the weight average molecular weight is too small, it is difficult to hold on the skin when applied to the skin and the feeling of use becomes worse.
It is desirable that the collagen peptide composition contains as little impurities as possible other than the collagen peptide. Examples of impurities include components contained in the extraction raw material, such as fats and oils, pigments, mucopolysaccharides, and the like. Some impurities are introduced during the manufacturing process.
When used in cosmetics and pharmaceuticals, if the peptide solution to be blended contains a large amount of fats and oils, the color will deteriorate and the fish odor and animal odor derived from the raw material will be strongly emitted, causing discomfort when applied to the skin. . Therefore, what contains as little fat as possible as a collagen containing raw material mentioned later is preferable.

The purity of the collagen peptide composition can be increased to necessary purity conditions by selecting production raw materials, setting production conditions, purification treatment, and the like.
The collagen peptide composition is usually provided in the form of an aqueous solution. It can also be provided as a solution in other solvents. It can also be provided in a solid state such as a powder or granule of a collagen peptide composition.
When the collagen peptide composition is made into an aqueous solution, the transparency as high as possible is suitable for cosmetics and the like. Specifically, a light transmittance of 90% (wavelength 400 nm) or more is preferable.

[Production of collagen peptide composition]
Basically, ordinary collagen peptide production techniques can be applied.
In order to obtain a collagen peptide composition satisfying specific molecular weight distribution conditions, a pre-manufactured collagen peptide is fractionated for each narrow molecular weight range, and the fractionated collagen peptides are combined at a predetermined blending ratio. Can do. However, although such a method can be carried out at the research test level, it is not very suitable for industrial production.
As a method suitable for industrial production, a method including the following steps can be employed.
<Preparation step of collagen-containing raw material (a)>
As the collagen-containing raw material, it is preferable to prepare a collagen-containing raw material selected from the group consisting of fish scales and animal Achilles tendons and having a fat content of less than 0.3%. When used in cosmetics and pharmaceuticals, raw materials with less oil and fat can eliminate unpleasant odors derived from oil and fat.

The fish scales are not particularly limited as long as the fish scales contain collagenous tissue. For example, examples of the fish species include spring bream, nile perch, carp, grass fish, red pine bream, lobster, and cod. Fish species with many scales that are easy to separate and recover from fish are easy to handle. Scales of fish species rich in collagen tissue contained in the scales and scales of fish species containing few unnecessary components contained in the scales are preferred.
Fish scales are used in a state separated from fish or skin. In order to remove dirt and impurities attached to fish scales, it is desirable to perform a washing process such as water washing. It is preferable to sufficiently remove dirt and contaminants by repeating the water washing step a plurality of times. Degreasing treatment can be performed to remove oil and fat. Demineralization treatment can remove inorganic substances such as phosphorus and calcium.

Animal Achilles tendon has a high content of collagen tissue among connective tissues of animals. There are few impurities such as fats and oils. As animals for extracting the Achilles tendon, mammals such as cows and pigs, and birds such as chickens, turkeys and ostriches are used. Mammals such as cattle have problems with BSE infection, whereas birds do not have such problems. Large birds such as turkeys and ostriches have a large amount of Achilles tendon that can be removed, making them suitable for industrial production.
In the case of the animal Achilles tendon, it is desirable to sufficiently remove unnecessary substances such as fats and oils by performing a water washing process, a degreasing process, and a deashing process as in the case of fish scales.
By using a collagen-containing raw material having a fat and oil content of less than 0.3%, a high-purity and high-performance collagen peptide composition can be efficiently obtained. Processing of filtration and purification after extracting the collagen peptide becomes easy.

In addition, the collagen containing raw material sent to the next alkali treatment process is preferably in a state where the collagen tissue is not divided as much as possible. Therefore, pulverization processing to fine particles is not performed. When the pulverization is performed, it becomes difficult to wash with water after the alkali treatment, and the refining work takes time. Usually, in the case of fish scales, individual scales may be used, and in the case of Achilles tendons, those taken out may be used.
<Alkali treatment step (b)>
Basically, techniques common to alkali treatment employed in normal collagen production or collagen peptide production can be applied.

Alkaline treatment removes impurities such as proteins and polysaccharides contained in the raw material, making the raw tissue swelled, cutting the bond between collagen and tissue so that the collagen peptide can be easily extracted from the collagen-containing raw material. There is a function to.
As the alkali treatment liquid, a lime liquid containing slaked lime (calcium hydroxide), a sodium hydroxide solution containing sodium sulfate, or the like can be used. The pulverized raw material pulverized in the previous step is put into such an alkali treatment liquid and immersed for a predetermined period. The pH of the treatment liquid can be set to 12.0 or higher.
As an alkali treatment liquid, a sodium hydroxide solution containing sodium sulfate is excellent in treatment performance. The sodium hydroxide concentration of the alkali treatment liquid can be set to about 0.3 to 6%. The sodium sulfate concentration can be set to about 10 to 20%.

The temperature of the alkaline treatment liquid can be set to 15 to 30 ° C. The processing period can be set to 5 to 20 days. During the treatment, the treatment liquid can be agitated or replaced.
When the alkali treatment is completed, water washing treatment or neutralization treatment can be performed. The water washing treatment removes alkali components adhering to the raw material. What is necessary is just to wash | clean the raw material taken out from the processing liquid of an alkali treatment with a lot of water, stirring. The neutralization treatment neutralizes the alkali component adhering to the raw material with an inorganic acid or the like. Alkali components that could not be removed by the water washing treatment can be neutralized. After the neutralization treatment, a further water washing treatment can be performed to remove excess acid used for neutralization.
<Enzyme treatment step (c)>
A collagen peptide composition having a target molecular weight distribution can be obtained by performing an enzyme treatment on the raw material after the alkali treatment.

Basically, it is the same as the extraction process using an enzyme in normal peptide production. A collagen-containing material is brought into contact with an enzyme to degrade collagen molecules to the peptide stage. Thereafter, if necessary, a solid-liquid separation treatment is performed to recover a water-soluble collagen peptide composition dissolved in the extract.
The collagen-containing raw material can be cut or pulverized before the enzyme treatment. Enzyme treatment can be efficiently performed by reducing the particle size of the collagen-containing raw material.
The enzyme may be an enzyme having a function of cleaving a peptide bond of a collagen molecule. Usually, it is an enzyme called a proteolytic enzyme or proathesis. Prothesis includes collagenase, cysteine protease, serine protease, aspartic protease and the like. Proteases may also be classified as acidic proteases, neutral proteases and alkaline proteases. These various proteases can be used singly or in combination.

Collagenase has a function of specifically cleaving the amino group side of glycine (Gly) in the amino acid sequence of the collagen molecule. Therefore, the collagen peptide composition obtained by enzyme treatment with collagenase has a large number of tripeptides or dipeptides beginning with Gly, and it is difficult to obtain a collagen peptide composition having the specific molecular weight distribution conditions described above. However, collagenase can be used as part of the enzyme treatment.
Known cysteine proteases include plant-derived chymopapain, papain, promelain, ficin, animal-derived cathepsins, calcium-dependent proteases, and the like. Any one of the enzymes may be used, or the enzyme treatment may be performed by combining a plurality of enzymes. Cysteine protease does not have a specific degradation function like collagenase. A collagen peptide composition having a target molecular weight distribution can be obtained by appropriately setting the treatment environment and treatment conditions for the enzyme treatment. Compared to collagenase, it is economically available, easy to handle, and has a high production efficiency of a peptide composition having a target molecular weight distribution. For industrial production, cysteine protease is preferred over collagenase.

Serine proteases such as trypsin and chymotrypsin and aspartic proteases such as pepsin and cathepsin D also have less specific degradation functions than collagenase.
The processing temperature is set to 20-80 ° C. Preferably, it can set to 35-80 degreeC. If the treatment temperature is too low, the function of the enzyme is not fully exhibited, and the target molecular weight distribution is difficult to obtain. If the treatment temperature is too high, the degradation of collagen by the enzyme proceeds too much, and it is difficult to achieve the target molecular weight distribution.
Set the processing time to 1-48 hours. If the processing time is short, the processing effect cannot be obtained. Even if the treatment time is too long, the treatment effect is not improved, and the collagen is decomposed too much.

<Recovery of collagen peptide composition>
The collagen peptide composition which is the object of the present invention is contained as a water-soluble substance dissolved in the treatment solution after the enzyme treatment. The aqueous collagen peptide composition solution contains almost no impurities other than the target collagen peptide composition.
Therefore, the obtained collagen peptide composition aqueous solution can be used as a product as it is. Further, if necessary, various purification treatments can be performed to obtain a product with higher purity. As a purification treatment, an aqueous solution of a collagen peptide composition and a solid unnecessary substance can be separated by performing various solid-liquid separation treatments employed in normal collagen peptide production technology. Examples of the solid-liquid separation process include a filtration process and a centrifugal separation process.

In general, an aqueous solution containing a sufficiently pure, colorless, and odorless collagen peptide composition can be obtained simply by subjecting the treatment solution after the enzyme treatment to a relatively simple purification treatment using activated carbon or the like. .
<Other processes>
Various processing steps and processing techniques employed in normal collagen peptide manufacturing technology can be combined as necessary.
For example, after the extraction step, a filtration step, a purification step, a concentration step, and the like can be performed. The same techniques as those used in normal collagen peptide production can be applied to the equipment used and the processing conditions for these processing steps.

[Use of collagen peptide composition]
Basically, it can be used in the same application and usage form as a normal collagen peptide or a composition thereof.
For example, cosmetic use, pharmaceutical and quasi-drug use, medical use body material use, cell culture substrate use, food use and the like.
The cosmetic composition used for cosmetic applications includes a case where it is a cosmetic product itself and a case where it is a raw material or an intermediate product for producing a cosmetic product. The cosmetic composition is usually an aqueous solution containing a collagen peptide composition.

The cosmetic composition can also contain components other than the collagen peptide composition. For example, water-soluble collagen, gelatin, collagen or the like having a molecular weight larger than that of the peptide can be combined. By combining a plurality of collagen components, there are cases in which functions and characteristics that cannot be obtained by a collagen peptide composition alone can be exhibited.
Other blending components can be arbitrarily designed depending on the purpose of use and required functions. For example, the moisture retention function of the collagen peptide composition can be further enhanced by blending water-soluble collagen, mucopolysaccharides such as hyaluronic acid, and salts thereof. In addition, examples of general additive materials for cosmetics include various oils and fats, alcohols, herbal medicines, animal ingredients, pigments, ultraviolet absorbers / blockers, fragrances, stabilizers, and the like.

Cosmetics that use cosmetic compositions include basic cosmetics such as cold cream, milky lotion and lotion, skin cosmetics such as lipsticks, hair cosmetics such as hair conditioners, facial cleansers, after-shave lotions, and other collagen. It can be used for various cosmetics in which the function of the peptide composition is effective.
For the specific formulation of cosmetics, techniques common to ordinary collagen-based cosmetics can be applied. In the cosmetic formulations described in various documents, the alkali-treated collagen of the present invention can be used in place of the collagen used therein. As a specific example, a cosmetic prescription disclosed in JP 2003-327599 A can be applied.

  As a pharmaceutical composition used for pharmaceutical applications, a collagen peptide composition can be used in a skin ointment, cream, skin wound recovery coating, and the like. Ingredients other than the collagen peptide composition can be appropriately set according to the purpose and medicinal effect of the pharmaceutical composition.

The collagen peptide composition according to the present invention has a specific molecular weight distribution, so that it can be applied to the skin as a cosmetic or pharmaceutical product for skin as compared with conventional collagen peptide compositions that deviate from such molecular weight distribution conditions. When used for the purpose of use, it has good elongation, does not flow excessively and does not spread, has no stickiness, and has a moist feeling and excellent use performance. In addition, the skin permeability inherent to the collagen peptide, the cell function improving effect, etc. can exhibit superior performance that is practically comparable to low molecular weight peptides less than tripeptides.
In the production method of the present invention, by performing alkali treatment and enzyme treatment on a specific raw material, a collagen peptide composition that satisfies the specific molecular weight distribution conditions as described above can be efficiently produced with high productivity.

The specific manufacture example of a collagen peptide composition and the result of having evaluated the performance are shown.
[Example 1]
<Preparation of raw materials>
A fresh ostrich Achilles tendon with a wet weight of 10 kg was used as a raw material.
The process of washing the raw material with water at 15 ° C. or lower was repeated three times. Subsequently, after adding the raw material to 50 L of 10% sodium chloride aqueous solution and stirring for 24 hours, the operation of separating the raw material was repeated three times to remove impurities such as blood. The obtained ostrich / Achilles tendon was added to 50 L of 5% ethylenediaminetetraacetic acid / sodium tetraacetate (EDTA) solution and stirred for 24 hours, and then the operation of separating the ostrich / Achilles tendon was repeated twice to remove inorganic substances such as calcium phosphate. did. Thereafter, washing with water was performed, and after adding ostrich / Achilles tendon to 20 L of ethanol at about 5 ° C. and stirring for 24 hours, the ostrich / Achilles tendon was separated and washed with water to perform a degreasing treatment.

Through these operations, a pre-treated ostrich Achilles tendon (wet weight 6.7 kg, dry weight 1.9 kg, oil content 0.05 wt%) was obtained.
<Alkali treatment>
A pretreated ostrich Achilles tendon having a dry weight of 2 kg was immersed in 20 L of a 1N sodium hydroxide solution containing 20% sodium sulfate, and kept at 20 ° C. for 10 days for alkali treatment.
After the alkali treatment, the alkali content was removed by washing with about 100 L of purified water.
<Enzyme treatment>
The alkali-treated ostrich Achilles tendon was pulverized to 1 cm or less with a cutting machine, and purified water twice the wet weight was added. Furthermore, 20 g of papain (manufactured by Amano Enzyme) was added, the temperature was kept at 50 ° C., and the enzyme reaction was performed for 24 hours while stirring with a stirrer.

After the enzyme reaction was sufficiently performed, the enzyme was inactivated by maintaining at 80 ° C. for 60 minutes.
<Purification treatment>
The aqueous solution containing the collagen peptide composition obtained in the previous step was treated with activated carbon and then filtered with filter paper to remove the activated carbon.
As a result, a collagen peptide solution 40L (concentration 7.1%) was finally obtained. The yield was 90.8% based on the collagen raw material before the alkali treatment.
Purified water was added to the collagen peptide solution to a concentration of 5% to obtain a collagen peptide solution having a concentration of 5%.

[Comparative Example 1]
In Example 1, except for omitting the alkali treatment step, a collagen peptide solution was obtained through the same production steps such as enzyme treatment and purification treatment.
A collagen peptide solution 40L (concentration 2.1%) was obtained. The yield based on the collagen raw material before the alkali treatment was 26.9%.
[Comparative Example 2]
In Example 1, a collagen peptide solution was obtained by the same production process as in Example 1 except that the enzyme used in the enzyme treatment was replaced by collagenase from papain.

A collagen peptide solution 40 L (concentration 7.5%) was obtained. The yield based on the collagen raw material before the alkali treatment was 95.9%.
[Comparative Example 3]
In Example 1, a collagen peptide solution was obtained by the same production process as in Example 1 except that collagenase was used for enzyme treatment without performing alkali treatment.
A collagen peptide solution 40 L (concentration 7.2%) was obtained. The yield based on the collagen raw material before the alkali treatment was 92.1%.
[Example 2]
In Example 1, a collagen peptide solution using shark scales as a raw material was obtained through the same production process as Example 1 except that 10 kg of fresh strawberry scales were used as a raw material.

As a result, a collagen peptide solution 40L (concentration 7.1%) was finally obtained. The yield was 90.8% based on the collagen raw material before the alkali treatment.
Purified water was added to the collagen peptide solution to a concentration of 5% to obtain a collagen peptide solution having a concentration of 5%.
[Comparative Example 4]
In Example 2, a collagen peptide solution was obtained through the same production steps such as enzyme treatment and purification treatment, except that the alkali treatment step was omitted.
A collagen peptide solution 40 L (concentration 2.4%) was obtained. The yield based on the collagen raw material before the alkali treatment was 30.1%.

[Comparative Example 5]
In Example 2, a collagen peptide solution was obtained by the same production process as in Example 2 except that the enzyme used in the enzyme treatment was replaced with collagenase.
A collagen peptide solution 40L (concentration 7.3%) was obtained. The yield based on the collagen raw material before the alkali treatment was 93.8%.
[Comparative Example 6]
In Example 2, a collagen peptide solution was obtained in the same production process as Example 2 except that collagenase was used for enzyme treatment without performing alkali treatment.

A collagen peptide solution 40 L (concentration 7.2%) was obtained. The yield based on the collagen raw material before the alkali treatment was 92.6%.
[Manufacture of cosmetics]
Using the collagen peptide solution obtained in each Example and Comparative Example, cosmetics were produced according to the following formulation. The concentration of the collagen peptide solution was adjusted by concentration or dilution so as to be 5% in all cases. Cosmetics are lotions applied to the skin. There was no particular problem in the production of cosmetics with any collagen peptide.
<Composition of cosmetics>
Collagen peptide solution (concentration 5%) 90% by weight
1,3-butylene glycol 5% by weight
Methyl paraoxybenzoate 0.1% by weight
4.9% by weight of water
[Performance evaluation]
<Molecular weight distribution, average molecular weight>
The molecular weight distribution and average molecular weight were measured by HPLC for the collagen peptide solutions obtained in each Example and Comparative Example.

HPLC measurement conditions were as follows: Column: TskGEL manufactured by Tosoh Corporation, G2500PWXL (column temperature 25 ° C.), eluent: 45% acetonitrile (0.1% TFA), flow rate 0.5 mL / min, detector: UV 218 nm.
The average molecular weight was measured by using GPC-8020 manufactured by Tosoh Corporation.
<Color, smell>
The collagen peptide solution was visually observed to evaluate the color and odor.
<Sensory test of cosmetics>
A sensory test of cosmetics was performed with a monitor (12 people aged 23 to 53).

Odor: The odor was judged when smelling cosmetics. Moreover, after applying cosmetics to skin and then wiping off cosmetics, the odor remaining on the skin was determined. The case where there was no smell was judged as good, and the case where there was a smell was judged as bad.
Stickiness: The stickiness was determined on the skin. A case where there was no stickiness or little discomfort was judged as good, and a case where the stickiness was strong and uncomfortable was judged as bad.
Moist feeling: Determined moist feeling on skin. The case where there was a moist feeling was judged as good, and the case where there was no moist feeling was judged as bad.
Each item was evaluated by the number of people judged to be good among 12 people.

〔Test results〕
The test results are shown in Tables 1 to 3 below. In the molecular weight distribution category of Table 1, “<” means less than and “> =” means more than.

<Evaluation>
(1) In the collagen peptide compositions of Examples 1 and 2, most of the peptides have a molecular weight of 400 to 3000, and less than 400 and 3000 or more contain only a small amount of peptides. In particular, peptides with a molecular weight of 400-1500 are the most abundant.
In contrast, the collagen peptide compositions of Comparative Examples 1-3, 4-6 contain a considerably large amount of peptides having a molecular weight of less than 400 or 3000 or more.
(2) In Comparative Examples 1 and 4, it can be presumed that only a peptide having a large molecular weight was obtained because the enzyme treatment with cysteine protease (papain) was performed without the alkali treatment. High molecular weight impurities that cause animal odor remain. When used in cosmetics, the sticky feeling becomes stronger and the moist feeling is inferior.

(3) In Comparative Examples 2 and 5, since collagenase was used for the enzyme treatment, a large amount of tripeptide having a small molecular weight was included. For this reason, there is a disadvantage that when used in cosmetics, the moist feeling is not good.
(4) In Comparative Examples 3 and 6, enzyme treatment with collagenase is performed without alkali treatment. The molecular weight distribution was not significantly different from Comparative Examples 2 and 5, but the odor remained. When combined with the results of Comparative Examples 1 and 4, it can be seen that performing the alkali treatment is effective for the non-bromination of the collagen peptide composition.
[Cosmetic prescription]
With the specific formulation shown below, it was possible to produce a cosmetic containing a collagen peptide composition. The collagen peptide was used in an aqueous solution of the collagen peptide composition of Example 1 or Example 2. In any case, the effects of the present invention could be satisfactorily exhibited.

<Cosmetic prescription example 1: lotion>
Collagen peptide (concentration 5%): 20.0 parts, water-soluble collagen, hyaluronic acid or chondroitin sulfate solution: 5.0 parts, perfume (lemon water): appropriate amount, preservative (phenoxyethanol): appropriate amount, deep sea water or birch sap : Remainder when the total amount of each component is 100 parts.

  The collagen peptide composition of this invention can be mix | blended with the cosmetics for skin attached to skin, for example. When the skin cosmetic is applied to the skin, it can be quickly and smoothly stretched to cover the skin thinly and uniformly. As a feeling of use, a moist and comfortable feeling can be given. It is highly permeable to the skin and can enhance the moisture retention function of the skin or achieve an improvement in cell function. Since it is easy to manufacture and can be produced economically, the commercial value of skin cosmetics can be increased.

Claims (5)

A composition of a collagen peptide that is a degradation product of collagen,
60 to 100% by weight of a peptide having a molecular weight of 400 or more and less than 3000,
Less than 25% by weight of peptides with a molecular weight of less than 400;
A collagen peptide composition comprising a peptide having a molecular weight of 3000 or more and less than 15% by weight. The collagen peptide composition according to claim 1, which has a weight average molecular weight of 600 to 1800. The collagen peptide composition according to claim 1 or 2, wherein the collagen is a collagen selected from the group consisting of fish scale-derived collagen and animal Achilles tendon-derived collagen. A method for producing a collagen peptide composition according to claims 1-3,
The collagen-containing raw material is selected from the group consisting of fish scales and animal Achilles tendons, and a collagen-containing raw material having a fat content of less than 0.3% (a),
A step (b) of subjecting the collagen-containing raw material to an alkali treatment;
A process for producing a collagen peptide composition comprising the step (c) of subjecting the alkali-treated raw material to an enzyme treatment using a protease at a temperature of 20 to 80 ° C. to obtain a collagen peptide composition. A cosmetic composition comprising the collagen peptide composition according to any one of claims 1 to 4.