WO2023128302A1 - Dispositif de prétraitement de virus - Google Patents

Dispositif de prétraitement de virus Download PDF

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Publication number
WO2023128302A1
WO2023128302A1 PCT/KR2022/018764 KR2022018764W WO2023128302A1 WO 2023128302 A1 WO2023128302 A1 WO 2023128302A1 KR 2022018764 W KR2022018764 W KR 2022018764W WO 2023128302 A1 WO2023128302 A1 WO 2023128302A1
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WO
WIPO (PCT)
Prior art keywords
cartridge
reservoirs
hole
sample
cartridge insertion
Prior art date
Application number
PCT/KR2022/018764
Other languages
English (en)
Korean (ko)
Inventor
천진우
이재현
정지용
Original Assignee
연세대학교 산학협력단
기초과학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020220155404A external-priority patent/KR20230101703A/ko
Application filed by 연세대학교 산학협력단, 기초과학연구원 filed Critical 연세대학교 산학협력단
Publication of WO2023128302A1 publication Critical patent/WO2023128302A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • the present invention relates to a virus pre-processing device, and more particularly, to a virus pre-processing device that performs pre-processing of a virus sample to be subjected to biomarker molecular diagnosis such as PCR (Polymerase Chain Reaction).
  • biomarker molecular diagnosis such as PCR (Polymerase Chain Reaction).
  • coronavirus infection COVID-19
  • COVID-19 coronavirus infection
  • researchers are warning that a large-scale epidemic of various types of viral infections may occur in the future in addition to corona virus infections.
  • corona virus infections In order to suppress the large-scale epidemic of various viral infections, including corona virus infections, as much as possible, it is effective to identify and isolate as many symptomatic or asymptomatic infected people as quickly as possible.
  • PCR-based nucleic acid amplification test (Nucleic-acid amplification test, NAAT) has high analytical accuracy ( ⁇ 99%) in virus detection and is known as an effective means for diagnosing various viral infections. .
  • RT-PCR reverse transcription PCR
  • pretreatment is required for samples containing viruses. More specifically, for safe and accurate diagnosis, it is necessary to preserve the nucleic acid inside the virus while removing the infectivity of the virus present in the sample.
  • pretreatment of the sample is generally performed by personnel performing the diagnosis, and contamination of the sample is likely to occur during the pretreatment of the sample.
  • devices that automatically perform sample pretreatment have been introduced, but the structure is complicated and the efficiency is low because the sample has a structure in which the sample is transferred several times for a plurality of pretreatment steps or, conversely, a plurality of pretreatment solutions are transferred to the sample respectively. I have a low problem.
  • the present invention has been made to solve the above problems, and an object of the present invention is to provide a virus pretreatment device that minimizes problems such as sample contamination by performing pretreatment of samples containing viruses inside the device.
  • Another object of the present invention is to provide a virus pre-processing device that efficiently performs sample pre-processing through a simple operation.
  • the body a cartridge insertion port formed inwardly from one side of the main body; a sample inlet passing through an upper surface of the main body and an upper sidewall of the cartridge insertion port; one or more reservoirs disposed at an upper portion of the cartridge insertion port and spaced apart from the sample input port, and having a lower portion communicating with the cartridge insertion port; a discharge passage spaced apart from the lower side of the one or more reservoirs, disposed below the cartridge insertion port, and communicating with the upper side of the cartridge insertion port; and a cartridge having a cartridge body slidably inserted into the cartridge insertion hole and a through hole vertically penetrating the cartridge body.
  • the through hole communicates with the sample inlet, the lower part of the through hole is closed by the lower side wall of the cartridge insertion hole, and the lower part of the one or more reservoirs is attached to the cartridge body.
  • a pretreatment solution for pretreatment of the sample may be stored in each of the one or more reservoirs, and the cartridge may enter the through hole.
  • the through hole is in communication with the one or more reservoirs and the discharge passage so that the pretreatment solution stored in the one or more reservoirs and the sample are mixed and flow into the discharge passage.
  • the cartridge body may have a square rod shape.
  • the one or more reservoirs may include a first reservoir capable of storing a first pretreatment solution; and a second reservoir capable of storing the second pretreatment solution.
  • first reservoir and the second reservoir may be disposed side by side along a direction perpendicular to the sliding direction of the cartridge.
  • the virus preprocessing device is fixed to the inside of the through-hole to provide a flow path passing vertically, and a pin unit capable of penetrating the lower part of the container when the container carrying the sample enters the inside of the through-hole.
  • the branch may further include a through-hole arranging member.
  • the one or more reservoirs may have open tops
  • the virus preprocessing device may further include one or more reservoir covers covering upper portions of the one or more reservoirs.
  • the main body, the sample inlet and the one or more reservoirs are disposed, a first part having an upper cartridge insertion hole forming part having an open lower part, disposed below the first part, and the discharge passage Is disposed, may include a second part having a lower cartridge insertion hole forming portion to close the open lower portion of the upper cartridge insertion hole forming portion in a state coupled to the first portion and form the cartridge insertion hole.
  • the virus pretreatment device allows the introduction of the sample and the mixing of the pretreatment solution for pretreatment of the sample to be completed in a two-step process inside the device through a structure in which the cartridge is inserted into the main body, , providing high reliability and efficiency in sample preparation.
  • FIG. 1 is a perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • FIG. 2 is an exploded perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • FIG. 3 is a diagram showing the operation of a virus pre-processing device according to an embodiment of the present invention.
  • FIG. 4 is a cross-sectional view showing arrangement of a sample inlet and through-holes of the cartridge when the cartridge of the virus pre-processing device is in the first position according to an embodiment of the present invention.
  • FIG. 5 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the first position of the virus preprocessing device according to an embodiment of the present invention.
  • FIG. 6 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the second position of the virus preprocessing device according to an embodiment of the present invention.
  • Words and terms used in this specification and claims are not construed as limited in their ordinary or dictionary meanings, but in accordance with the principle that the inventors can define terms and concepts in order to best describe their inventions. It should be interpreted as a meaning and concept that corresponds to the technical idea. Since the embodiments described in this specification and the configurations shown in the drawings correspond to a preferred embodiment of the present invention and do not represent all of the technical ideas of the present invention, the corresponding configurations are various equivalents to replace them at the time of filing of the present invention. There may be water and variations.
  • a component being in the "front”, “rear”, “above” or “below” of another component means that it is in direct contact with another component, unless there are special circumstances, and is “in front”, “rear”, “above” or “below”. It includes not only those disposed at the lower part, but also cases in which another component is disposed in the middle.
  • the fact that certain components are “connected” to other components includes cases where they are not only directly connected to each other but also indirectly connected to each other unless there are special circumstances.
  • FIG. 1 is a perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • 2 is an exploded perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • the virus pre-processing apparatus 100 performs pre-processing of a virus-containing sample.
  • the sample may be a sample for PCR (Polymerase Chain Reaction). More specifically, the sample is subject to nucleic acid detection through PCR, and may be prepared by purifying RNA or DNA from saliva of a subject.
  • the nucleic acid to be detected may be the N1 and N2 genes for detecting the SARS-CoV-2 virus and the human RPP30 gene for confirming that the sample is a human sample.
  • the nucleic acid to be detected may vary depending on the type of infection to be diagnosed.
  • the virus preprocessing device 100 includes a main body 110, a cartridge insertion port 120, a sample inlet 130, one or more reservoirs 140a and 140b, It may include a reservoir cover 150, a discharge passage 160, a cartridge 170, and a through hole arranging member 180.
  • the main body 110 is provided so that the cartridge 170 for inputting and transporting the sample is inserted, and components for storing the pretreatment solution for pretreatment of the sample and components for discharging the pretreated sample are disposed.
  • the main body 110 may have a rectangular box shape.
  • the main body 110 has a sample inlet 130 and the one or more reservoirs 140a and 140b disposed, and has an upper cartridge insertion port forming portion 120a with an open bottom at the lower side.
  • the first part 110a and the lower side of the first part 110a, the discharge passage 160 is disposed, and the opening of the upper cartridge insertion port forming part 120a in a state coupled with the first part 110a.
  • It may include a second part (110b) having a lower cartridge insertion hole forming portion (120b) closing the lower portion and forming the cartridge insertion hole (120).
  • Cartridge insertion port 120 is formed inward from one side of the main body 110.
  • a cartridge 170 is inserted into the cartridge insertion port 120 .
  • the cartridge 170 can be slidably displaced between the first position and the second position inside the cartridge insertion port 120 .
  • the cartridge insertion hole 120 is the first part (110a) and the second part (110b) of the main body 110 are combined to form the main body 110, the first part of the main body 110 It may be formed by the upper cartridge insertion hole forming part 120a of the first part 110a and the lower cartridge insertion hole forming part 120b of the second part 110b of the main body 110.
  • the sample inlet 130 passes through the top surface of the main body 110 and the upper sidewall of the cartridge insertion hole 120 .
  • the sample inlet 130 may be formed on one side of the main body 110 at an upper portion spaced apart by a predetermined interval along the cartridge entry direction.
  • the sample inlet 130 may have a cylindrical shape extending up and down.
  • the sample may be prepared in the form of a mixture of the subject's saliva and the dilution liquid, and a container containing the mixture of the subject's saliva and the dilution liquid may be introduced through the sample inlet 130 .
  • a sample such as saliva is directly introduced through the sample inlet 130 .
  • One or more reservoirs (140a, 140b) is disposed spaced apart from the sample inlet 130 on the upper portion of the cartridge insertion port 120, the lower portion communicates with the cartridge insertion port (120).
  • One or more reservoirs (140a, 140b) may be disposed at points spaced apart by a predetermined interval along the entry direction of the cartridge in the sample inlet (130).
  • One or more reservoirs may include a first reservoir 140a and a second reservoir 140b.
  • the first reservoir 140a may store the first pretreatment solution when the cartridge 170 is in the first position.
  • the second reservoir 140b may store the second pretreatment solution when the cartridge 170 is in the first position.
  • the first pretreatment solution may be a solution that removes the infectivity of the virus included in the sample and preserves the internal nucleic acid (eg, RNA) of the virus.
  • the second pretreatment solution may be a solution that dissolves the virus and removes a degrading enzyme (eg, RNA degrading enzyme) that degrades nucleic acids inside the virus.
  • the second pretreatment solution may include salt.
  • the first reservoir 140a communicates with the first reservoir 141a in which the first pretreatment solution can be stored and the first reservoir 141a communicates with the cartridge insertion port 120
  • 1 includes a first communication part 142a extending from the lower part of the storage part 141a to the upper side wall of the cartridge insertion port 120
  • the second reservoir 140b communicates with the second storage unit 141b in which the second pretreatment solution can be stored and the second storage unit 141b communicates with the cartridge insertion port 120
  • the second storage unit 141b It includes a second communication portion (142b) extending from the lower part to the upper sidewall of the cartridge insertion hole (120).
  • first communication portion 142a and the second communication portion 142b may be formed to be narrower than the first storage portion 141a and the second storage portion 141b, respectively.
  • the first communication portion 142a and the second communication portion 142b may be closed by a cartridge body 171 of a cartridge to be described later, and the first communication portion 142a and the second communication portion 142b are connected to each other of the cartridge.
  • a first pretreatment solution and a second pretreatment solution may be respectively stored in the first reservoir 140a and the second reservoir 140b in a closed state by the cartridge body 171 .
  • the first reservoir 140a and the second reservoir 140b may be disposed side by side. More specifically, the first reservoir 140a and the second reservoir 140b may be arranged side by side in a direction perpendicular to the entry direction of the cartridge. In other words, the first reservoir 140a and the second reservoir 140b are disposed at points spaced apart from the sample inlet 130 by a predetermined interval along the entry direction of the cartridge, and are parallel to the entry direction of the cartridge and perpendicular to the direction. They can be sorted and placed.
  • one or more reservoirs may have an open top. That is, each of the first reservoir 140a and the second reservoir 140b may be formed with an open top. Such an open structure provides an advantageous effect such as washing the first reservoir 140a and the second reservoir 140b with the first pretreatment solution and the second pretreatment solution.
  • the reservoir cover 150 covers the top of one or more reservoirs 140a and 140b. When each of the one or more reservoirs is filled with the pretreatment solution, the open top of the one or more reservoirs is preferably closed to prevent contamination of the pretreatment solution. The reservoir cover 150 closes the open top of one or more reservoirs while the pretreatment solution is filled in the one or more reservoirs.
  • the reservoir cover 150 covers both the first reservoir 140a and the second reservoir 140b.
  • a separate reservoir cover may be provided for each reservoir, but as in one embodiment of the present invention, when a plurality of reservoirs are simultaneously covered by one reservoir cover 150, efficiency is improved when the reservoir cover 150 is placed.
  • the reservoir cover 150 includes a cover body 151 covering the open state portions of the first reservoir 140a and the second reservoir 140b, and the cover body 151 includes the first reservoir ( 140a) and the second reservoir 140b, the first vent hole 152a and the second vent hole 152b for controlling internal pressure may be formed through the cover body 151.
  • the discharge flow passage 160 is spaced apart from the lower side of one or more reservoirs 140a and 140b and is disposed below the cartridge insertion port 120 and communicates with the upper side of the cartridge insertion port 120 .
  • the discharge passage 160 may be closed by the cartridge body 171 of the cartridge 170 so that communication with one or more reservoirs 140a and 140b is blocked, and the through hole 172 of the cartridge 170 may be blocked. Through this, it may be in a state of communication with one or more reservoirs 140a and 140b.
  • the sample mixed with the pretreatment solution may be discharged to the outside through the discharge passage 160.
  • the sample discharged to the outside may be transferred to a PCR device.
  • the discharge passage 160 communicates with the cartridge insertion port 120 and extends in the vertical direction with the first passage portion 161, the first passage portion 161 and the outside communicate with the side It may include a second flow path portion 162 extending to .
  • the cross-sectional area of the first flow path portion 161 may be larger than the cross-sectional area of the second flow path portion 162 .
  • the cartridge 170 is slidably inserted into the cartridge insertion port 120 .
  • the cartridge 170 includes a cartridge body 171 slidably inserted into the cartridge insertion port 120 and a through hole 172 vertically penetrating the cartridge body 171 .
  • the cartridge body 171 may have a square bar shape.
  • the cartridge 170 receives the sample introduced through the inlet 130 and transfers the sample to one or more reservoirs.
  • the cartridge 170 closes the lower part of the one or more reservoirs 140a and 140b through the cartridge body 171 in the first position so that the pretreatment solution can be maintained in a stored state, and the one or more reservoirs 140a and 140b ) and the discharge passage 160 are blocked.
  • the cartridge 170 opens the lower part of one or more reservoirs through the through hole 172 at the second position and communicates the one or more reservoirs 140a and 140b with the discharge passage 160 so that the sample and the pretreatment solution are mixed. It allows to be discharged to the outside through the discharge passage (160).
  • the through-hole arranging member 180 is disposed in the through-hole 172 of the cartridge 170, and when a container carrying the sample enters the through-hole 172, the sample penetrates the lower portion of the container through the through-hole ( 172) to allow it to flow into the interior.
  • the through-hole arranging member 180 is a placement body portion 181 fixedly mounted inside the through-hole 172, and a flow passage that vertically penetrates the placement body portion 181 to provide a flow path.
  • the pin part 183 connected to the body part 181 may be included so as to pass through the lower part of the container.
  • FIG. 3 is a diagram showing the operation of a virus pre-processing device according to an embodiment of the present invention.
  • 4 is a cross-sectional view showing the arrangement of a sample inlet and through-holes of the cartridge when the cartridge of the virus pre-processing device is in the first position according to an embodiment of the present invention
  • FIG. 5 is a virus pre-processing device according to an embodiment of the present invention.
  • 6 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the second position of the virus preprocessing device according to an embodiment of the present invention.
  • the cartridge 170 is inserted into the cartridge insertion hole 120 (FIG. 3(a)), and the cartridge 170 is placed in a first position (FIG. 3(b)).
  • the through hole 172 of the cartridge 170 communicates with the sample inlet 130 formed in the main body 110, and the cartridge body 171 is connected to the first reservoir 140a and the second reservoir 140b. It means a position where the lower part of ) is closed and communication between the first reservoir 140a and the second reservoir 140b and the discharge passage 160 is blocked.
  • the container 5 holding the sample S enters through the sample inlet 130 and passes through the through hole 172 of the cartridge 170. can reach At this time, the sample (S) may exist in a diluted state by the diluent in the container (5).
  • the lower part of the container 5 is penetrated by the pin 183 of the through hole arranging member 180, and the sample S existing inside the container 5 is passed through the through hole 172. leaked into the
  • the lower part of the through hole 172 is formed on the lower sidewall of the cartridge insertion hole 120, that is, the lower cartridge insertion hole forming part 120b of the second part 110b of the main body 110. Since the state is closed by, the sample (S) can be accommodated inside the through hole (172).
  • the cartridge body 171 blocks the first and second reservoirs 140a and 140b and the discharge passage 160 .
  • the first pretreatment solution may be a solution that removes the infectivity of the virus contained in the sample and preserves the internal nucleic acid (eg, RNA) of the virus.
  • the second pretreatment solution may be a solution that dissolves the virus and removes a degrading enzyme (eg, RNA degrading enzyme) that degrades nucleic acids inside the virus.
  • the cartridge 170 is slidably displaced from the first position to the second position (Fig. 3(c)).
  • the through hole 172 of the cartridge 170 opens the lower portions of the first reservoir 140a and the second reservoir 140b, and the first reservoir 140a and the second reservoir 140b are opened. This is a position where it communicates with the discharge passage 160.
  • the through hole 172 of the cartridge is disposed below the first reservoir 140a and the second reservoir 140b, and the first reservoir The first communication part 142a and the second communication part 142b of the bottom 140a and the second reservoir 140b are opened.
  • the first reservoir 140a and the second reservoir 140b communicate with the discharge passage 160 through the through hole 172 .
  • the first pretreatment solution (S1) stored in the first reservoir (140a) and the second pretreatment solution (S2) stored in the second reservoir (140b) enter the through hole 172 and mix with the sample (S). and the mixed solution (S+S1+S2) of the sample, the first pretreatment solution, and the second pretreatment solution is discharged to the outside through the discharge passage 160.
  • the through hole 172 communicates with the sample inlet 130, and the lower part of the through hole 172 is formed by the lower sidewall of the cartridge insertion hole 120. It is closed, and the lower part of the one or more reservoirs 140a and 140b is closed by the cartridge body 171, so that the biomarker molecular diagnostic sample S enters the through hole 172 through the sample inlet 130. It is possible, and a pretreatment solution for pretreatment of a sample may be stored in one or more reservoirs 140a and 140b, respectively.
  • the through hole 172 is in communication with the one or more reservoirs 140a and 140b and the discharge passage 160 so that the one or more reservoirs 140a and 140b communicate with each other.
  • the pretreatment solution stored in (140a, 140b) and the sample may be mixed and flow into the discharge channel 160.
  • the sample discharged to the outside through the discharge channel 160 may be transferred to a biomarker molecular diagnosis device such as a PCR device.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un dispositif de prétraitement de virus est divulgué. Un dispositif de prétraitement de virus selon un mode de réalisation de la présente invention peut comprendre : un corps principal ; un orifice d'insertion de cartouche, formé vers l'intérieur à partir d'un côté du corps principal ; un orifice d'entrée d'échantillons, qui pénètre dans une surface supérieure du corps principal et dans une paroi latérale supérieure de l'orifice d'insertion de cartouche ; un ou plusieurs réservoirs, disposés au sommet de l'orifice d'insertion de cartouche, afin d'être espacés de l'orifice d'entrée d'échantillon, et dont la partie inférieure communique avec l'orifice d'insertion de cartouche ; un passage d'évacuation, disposé au-dessous de l'orifice d'insertion de cartouche, afin d'être espacé vers le bas à partir du ou des réservoirs, et dont le côté supérieur communique avec l'orifice d'insertion de cartouche ; et une cartouche, dont un corps est inséré de façon coulissante dans l'orifice d'insertion de cartouche, ainsi qu'un trou traversant, qui pénètre verticalement dans le corps de cartouche.
PCT/KR2022/018764 2021-12-29 2022-11-24 Dispositif de prétraitement de virus WO2023128302A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR10-2021-0191806 2021-12-29
KR10-2021-0191807 2021-12-29
KR20210191806 2021-12-29
KR20210191807 2021-12-29
KR1020220155404A KR20230101703A (ko) 2021-12-29 2022-11-18 바이러스 전처리 장치
KR10-2022-0155404 2022-11-18

Publications (1)

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WO2023128302A1 true WO2023128302A1 (fr) 2023-07-06

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PCT/KR2022/018764 WO2023128302A1 (fr) 2021-12-29 2022-11-24 Dispositif de prétraitement de virus

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WO (1) WO2023128302A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4623716B2 (ja) * 2004-11-25 2011-02-02 旭化成株式会社 核酸検出用カートリッジ及び核酸検出方法
KR101630784B1 (ko) * 2014-09-24 2016-06-15 한국기계연구원 시료 전처리용 카트리지
KR20180125816A (ko) * 2017-05-16 2018-11-26 에스케이텔레콤 주식회사 카트리지를 이용한 핵산 분석 장치
KR101967236B1 (ko) * 2017-05-16 2019-04-09 에스케이텔레콤 주식회사 핵산 추출용 전처리 챔버, 그를 이용한 카트리지 및 핵산 추출 방법
KR20200018475A (ko) * 2017-06-21 2020-02-19 베링거잉겔하임베트메디카게엠베하 샘플을 전처리하기 위한 압축성 추출 기구

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4623716B2 (ja) * 2004-11-25 2011-02-02 旭化成株式会社 核酸検出用カートリッジ及び核酸検出方法
KR101630784B1 (ko) * 2014-09-24 2016-06-15 한국기계연구원 시료 전처리용 카트리지
KR20180125816A (ko) * 2017-05-16 2018-11-26 에스케이텔레콤 주식회사 카트리지를 이용한 핵산 분석 장치
KR101967236B1 (ko) * 2017-05-16 2019-04-09 에스케이텔레콤 주식회사 핵산 추출용 전처리 챔버, 그를 이용한 카트리지 및 핵산 추출 방법
KR20200018475A (ko) * 2017-06-21 2020-02-19 베링거잉겔하임베트메디카게엠베하 샘플을 전처리하기 위한 압축성 추출 기구

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