WO2023128302A1 - Virus pre-processing device - Google Patents

Virus pre-processing device Download PDF

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Publication number
WO2023128302A1
WO2023128302A1 PCT/KR2022/018764 KR2022018764W WO2023128302A1 WO 2023128302 A1 WO2023128302 A1 WO 2023128302A1 KR 2022018764 W KR2022018764 W KR 2022018764W WO 2023128302 A1 WO2023128302 A1 WO 2023128302A1
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WO
WIPO (PCT)
Prior art keywords
cartridge
reservoirs
hole
sample
cartridge insertion
Prior art date
Application number
PCT/KR2022/018764
Other languages
French (fr)
Korean (ko)
Inventor
천진우
이재현
정지용
Original Assignee
연세대학교 산학협력단
기초과학연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from KR1020220155404A external-priority patent/KR20230101703A/en
Application filed by 연세대학교 산학협력단, 기초과학연구원 filed Critical 연세대학교 산학협력단
Publication of WO2023128302A1 publication Critical patent/WO2023128302A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • the present invention relates to a virus pre-processing device, and more particularly, to a virus pre-processing device that performs pre-processing of a virus sample to be subjected to biomarker molecular diagnosis such as PCR (Polymerase Chain Reaction).
  • biomarker molecular diagnosis such as PCR (Polymerase Chain Reaction).
  • coronavirus infection COVID-19
  • COVID-19 coronavirus infection
  • researchers are warning that a large-scale epidemic of various types of viral infections may occur in the future in addition to corona virus infections.
  • corona virus infections In order to suppress the large-scale epidemic of various viral infections, including corona virus infections, as much as possible, it is effective to identify and isolate as many symptomatic or asymptomatic infected people as quickly as possible.
  • PCR-based nucleic acid amplification test (Nucleic-acid amplification test, NAAT) has high analytical accuracy ( ⁇ 99%) in virus detection and is known as an effective means for diagnosing various viral infections. .
  • RT-PCR reverse transcription PCR
  • pretreatment is required for samples containing viruses. More specifically, for safe and accurate diagnosis, it is necessary to preserve the nucleic acid inside the virus while removing the infectivity of the virus present in the sample.
  • pretreatment of the sample is generally performed by personnel performing the diagnosis, and contamination of the sample is likely to occur during the pretreatment of the sample.
  • devices that automatically perform sample pretreatment have been introduced, but the structure is complicated and the efficiency is low because the sample has a structure in which the sample is transferred several times for a plurality of pretreatment steps or, conversely, a plurality of pretreatment solutions are transferred to the sample respectively. I have a low problem.
  • the present invention has been made to solve the above problems, and an object of the present invention is to provide a virus pretreatment device that minimizes problems such as sample contamination by performing pretreatment of samples containing viruses inside the device.
  • Another object of the present invention is to provide a virus pre-processing device that efficiently performs sample pre-processing through a simple operation.
  • the body a cartridge insertion port formed inwardly from one side of the main body; a sample inlet passing through an upper surface of the main body and an upper sidewall of the cartridge insertion port; one or more reservoirs disposed at an upper portion of the cartridge insertion port and spaced apart from the sample input port, and having a lower portion communicating with the cartridge insertion port; a discharge passage spaced apart from the lower side of the one or more reservoirs, disposed below the cartridge insertion port, and communicating with the upper side of the cartridge insertion port; and a cartridge having a cartridge body slidably inserted into the cartridge insertion hole and a through hole vertically penetrating the cartridge body.
  • the through hole communicates with the sample inlet, the lower part of the through hole is closed by the lower side wall of the cartridge insertion hole, and the lower part of the one or more reservoirs is attached to the cartridge body.
  • a pretreatment solution for pretreatment of the sample may be stored in each of the one or more reservoirs, and the cartridge may enter the through hole.
  • the through hole is in communication with the one or more reservoirs and the discharge passage so that the pretreatment solution stored in the one or more reservoirs and the sample are mixed and flow into the discharge passage.
  • the cartridge body may have a square rod shape.
  • the one or more reservoirs may include a first reservoir capable of storing a first pretreatment solution; and a second reservoir capable of storing the second pretreatment solution.
  • first reservoir and the second reservoir may be disposed side by side along a direction perpendicular to the sliding direction of the cartridge.
  • the virus preprocessing device is fixed to the inside of the through-hole to provide a flow path passing vertically, and a pin unit capable of penetrating the lower part of the container when the container carrying the sample enters the inside of the through-hole.
  • the branch may further include a through-hole arranging member.
  • the one or more reservoirs may have open tops
  • the virus preprocessing device may further include one or more reservoir covers covering upper portions of the one or more reservoirs.
  • the main body, the sample inlet and the one or more reservoirs are disposed, a first part having an upper cartridge insertion hole forming part having an open lower part, disposed below the first part, and the discharge passage Is disposed, may include a second part having a lower cartridge insertion hole forming portion to close the open lower portion of the upper cartridge insertion hole forming portion in a state coupled to the first portion and form the cartridge insertion hole.
  • the virus pretreatment device allows the introduction of the sample and the mixing of the pretreatment solution for pretreatment of the sample to be completed in a two-step process inside the device through a structure in which the cartridge is inserted into the main body, , providing high reliability and efficiency in sample preparation.
  • FIG. 1 is a perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • FIG. 2 is an exploded perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • FIG. 3 is a diagram showing the operation of a virus pre-processing device according to an embodiment of the present invention.
  • FIG. 4 is a cross-sectional view showing arrangement of a sample inlet and through-holes of the cartridge when the cartridge of the virus pre-processing device is in the first position according to an embodiment of the present invention.
  • FIG. 5 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the first position of the virus preprocessing device according to an embodiment of the present invention.
  • FIG. 6 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the second position of the virus preprocessing device according to an embodiment of the present invention.
  • Words and terms used in this specification and claims are not construed as limited in their ordinary or dictionary meanings, but in accordance with the principle that the inventors can define terms and concepts in order to best describe their inventions. It should be interpreted as a meaning and concept that corresponds to the technical idea. Since the embodiments described in this specification and the configurations shown in the drawings correspond to a preferred embodiment of the present invention and do not represent all of the technical ideas of the present invention, the corresponding configurations are various equivalents to replace them at the time of filing of the present invention. There may be water and variations.
  • a component being in the "front”, “rear”, “above” or “below” of another component means that it is in direct contact with another component, unless there are special circumstances, and is “in front”, “rear”, “above” or “below”. It includes not only those disposed at the lower part, but also cases in which another component is disposed in the middle.
  • the fact that certain components are “connected” to other components includes cases where they are not only directly connected to each other but also indirectly connected to each other unless there are special circumstances.
  • FIG. 1 is a perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • 2 is an exploded perspective view of a virus pre-processing device according to an embodiment of the present invention.
  • the virus pre-processing apparatus 100 performs pre-processing of a virus-containing sample.
  • the sample may be a sample for PCR (Polymerase Chain Reaction). More specifically, the sample is subject to nucleic acid detection through PCR, and may be prepared by purifying RNA or DNA from saliva of a subject.
  • the nucleic acid to be detected may be the N1 and N2 genes for detecting the SARS-CoV-2 virus and the human RPP30 gene for confirming that the sample is a human sample.
  • the nucleic acid to be detected may vary depending on the type of infection to be diagnosed.
  • the virus preprocessing device 100 includes a main body 110, a cartridge insertion port 120, a sample inlet 130, one or more reservoirs 140a and 140b, It may include a reservoir cover 150, a discharge passage 160, a cartridge 170, and a through hole arranging member 180.
  • the main body 110 is provided so that the cartridge 170 for inputting and transporting the sample is inserted, and components for storing the pretreatment solution for pretreatment of the sample and components for discharging the pretreated sample are disposed.
  • the main body 110 may have a rectangular box shape.
  • the main body 110 has a sample inlet 130 and the one or more reservoirs 140a and 140b disposed, and has an upper cartridge insertion port forming portion 120a with an open bottom at the lower side.
  • the first part 110a and the lower side of the first part 110a, the discharge passage 160 is disposed, and the opening of the upper cartridge insertion port forming part 120a in a state coupled with the first part 110a.
  • It may include a second part (110b) having a lower cartridge insertion hole forming portion (120b) closing the lower portion and forming the cartridge insertion hole (120).
  • Cartridge insertion port 120 is formed inward from one side of the main body 110.
  • a cartridge 170 is inserted into the cartridge insertion port 120 .
  • the cartridge 170 can be slidably displaced between the first position and the second position inside the cartridge insertion port 120 .
  • the cartridge insertion hole 120 is the first part (110a) and the second part (110b) of the main body 110 are combined to form the main body 110, the first part of the main body 110 It may be formed by the upper cartridge insertion hole forming part 120a of the first part 110a and the lower cartridge insertion hole forming part 120b of the second part 110b of the main body 110.
  • the sample inlet 130 passes through the top surface of the main body 110 and the upper sidewall of the cartridge insertion hole 120 .
  • the sample inlet 130 may be formed on one side of the main body 110 at an upper portion spaced apart by a predetermined interval along the cartridge entry direction.
  • the sample inlet 130 may have a cylindrical shape extending up and down.
  • the sample may be prepared in the form of a mixture of the subject's saliva and the dilution liquid, and a container containing the mixture of the subject's saliva and the dilution liquid may be introduced through the sample inlet 130 .
  • a sample such as saliva is directly introduced through the sample inlet 130 .
  • One or more reservoirs (140a, 140b) is disposed spaced apart from the sample inlet 130 on the upper portion of the cartridge insertion port 120, the lower portion communicates with the cartridge insertion port (120).
  • One or more reservoirs (140a, 140b) may be disposed at points spaced apart by a predetermined interval along the entry direction of the cartridge in the sample inlet (130).
  • One or more reservoirs may include a first reservoir 140a and a second reservoir 140b.
  • the first reservoir 140a may store the first pretreatment solution when the cartridge 170 is in the first position.
  • the second reservoir 140b may store the second pretreatment solution when the cartridge 170 is in the first position.
  • the first pretreatment solution may be a solution that removes the infectivity of the virus included in the sample and preserves the internal nucleic acid (eg, RNA) of the virus.
  • the second pretreatment solution may be a solution that dissolves the virus and removes a degrading enzyme (eg, RNA degrading enzyme) that degrades nucleic acids inside the virus.
  • the second pretreatment solution may include salt.
  • the first reservoir 140a communicates with the first reservoir 141a in which the first pretreatment solution can be stored and the first reservoir 141a communicates with the cartridge insertion port 120
  • 1 includes a first communication part 142a extending from the lower part of the storage part 141a to the upper side wall of the cartridge insertion port 120
  • the second reservoir 140b communicates with the second storage unit 141b in which the second pretreatment solution can be stored and the second storage unit 141b communicates with the cartridge insertion port 120
  • the second storage unit 141b It includes a second communication portion (142b) extending from the lower part to the upper sidewall of the cartridge insertion hole (120).
  • first communication portion 142a and the second communication portion 142b may be formed to be narrower than the first storage portion 141a and the second storage portion 141b, respectively.
  • the first communication portion 142a and the second communication portion 142b may be closed by a cartridge body 171 of a cartridge to be described later, and the first communication portion 142a and the second communication portion 142b are connected to each other of the cartridge.
  • a first pretreatment solution and a second pretreatment solution may be respectively stored in the first reservoir 140a and the second reservoir 140b in a closed state by the cartridge body 171 .
  • the first reservoir 140a and the second reservoir 140b may be disposed side by side. More specifically, the first reservoir 140a and the second reservoir 140b may be arranged side by side in a direction perpendicular to the entry direction of the cartridge. In other words, the first reservoir 140a and the second reservoir 140b are disposed at points spaced apart from the sample inlet 130 by a predetermined interval along the entry direction of the cartridge, and are parallel to the entry direction of the cartridge and perpendicular to the direction. They can be sorted and placed.
  • one or more reservoirs may have an open top. That is, each of the first reservoir 140a and the second reservoir 140b may be formed with an open top. Such an open structure provides an advantageous effect such as washing the first reservoir 140a and the second reservoir 140b with the first pretreatment solution and the second pretreatment solution.
  • the reservoir cover 150 covers the top of one or more reservoirs 140a and 140b. When each of the one or more reservoirs is filled with the pretreatment solution, the open top of the one or more reservoirs is preferably closed to prevent contamination of the pretreatment solution. The reservoir cover 150 closes the open top of one or more reservoirs while the pretreatment solution is filled in the one or more reservoirs.
  • the reservoir cover 150 covers both the first reservoir 140a and the second reservoir 140b.
  • a separate reservoir cover may be provided for each reservoir, but as in one embodiment of the present invention, when a plurality of reservoirs are simultaneously covered by one reservoir cover 150, efficiency is improved when the reservoir cover 150 is placed.
  • the reservoir cover 150 includes a cover body 151 covering the open state portions of the first reservoir 140a and the second reservoir 140b, and the cover body 151 includes the first reservoir ( 140a) and the second reservoir 140b, the first vent hole 152a and the second vent hole 152b for controlling internal pressure may be formed through the cover body 151.
  • the discharge flow passage 160 is spaced apart from the lower side of one or more reservoirs 140a and 140b and is disposed below the cartridge insertion port 120 and communicates with the upper side of the cartridge insertion port 120 .
  • the discharge passage 160 may be closed by the cartridge body 171 of the cartridge 170 so that communication with one or more reservoirs 140a and 140b is blocked, and the through hole 172 of the cartridge 170 may be blocked. Through this, it may be in a state of communication with one or more reservoirs 140a and 140b.
  • the sample mixed with the pretreatment solution may be discharged to the outside through the discharge passage 160.
  • the sample discharged to the outside may be transferred to a PCR device.
  • the discharge passage 160 communicates with the cartridge insertion port 120 and extends in the vertical direction with the first passage portion 161, the first passage portion 161 and the outside communicate with the side It may include a second flow path portion 162 extending to .
  • the cross-sectional area of the first flow path portion 161 may be larger than the cross-sectional area of the second flow path portion 162 .
  • the cartridge 170 is slidably inserted into the cartridge insertion port 120 .
  • the cartridge 170 includes a cartridge body 171 slidably inserted into the cartridge insertion port 120 and a through hole 172 vertically penetrating the cartridge body 171 .
  • the cartridge body 171 may have a square bar shape.
  • the cartridge 170 receives the sample introduced through the inlet 130 and transfers the sample to one or more reservoirs.
  • the cartridge 170 closes the lower part of the one or more reservoirs 140a and 140b through the cartridge body 171 in the first position so that the pretreatment solution can be maintained in a stored state, and the one or more reservoirs 140a and 140b ) and the discharge passage 160 are blocked.
  • the cartridge 170 opens the lower part of one or more reservoirs through the through hole 172 at the second position and communicates the one or more reservoirs 140a and 140b with the discharge passage 160 so that the sample and the pretreatment solution are mixed. It allows to be discharged to the outside through the discharge passage (160).
  • the through-hole arranging member 180 is disposed in the through-hole 172 of the cartridge 170, and when a container carrying the sample enters the through-hole 172, the sample penetrates the lower portion of the container through the through-hole ( 172) to allow it to flow into the interior.
  • the through-hole arranging member 180 is a placement body portion 181 fixedly mounted inside the through-hole 172, and a flow passage that vertically penetrates the placement body portion 181 to provide a flow path.
  • the pin part 183 connected to the body part 181 may be included so as to pass through the lower part of the container.
  • FIG. 3 is a diagram showing the operation of a virus pre-processing device according to an embodiment of the present invention.
  • 4 is a cross-sectional view showing the arrangement of a sample inlet and through-holes of the cartridge when the cartridge of the virus pre-processing device is in the first position according to an embodiment of the present invention
  • FIG. 5 is a virus pre-processing device according to an embodiment of the present invention.
  • 6 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the second position of the virus preprocessing device according to an embodiment of the present invention.
  • the cartridge 170 is inserted into the cartridge insertion hole 120 (FIG. 3(a)), and the cartridge 170 is placed in a first position (FIG. 3(b)).
  • the through hole 172 of the cartridge 170 communicates with the sample inlet 130 formed in the main body 110, and the cartridge body 171 is connected to the first reservoir 140a and the second reservoir 140b. It means a position where the lower part of ) is closed and communication between the first reservoir 140a and the second reservoir 140b and the discharge passage 160 is blocked.
  • the container 5 holding the sample S enters through the sample inlet 130 and passes through the through hole 172 of the cartridge 170. can reach At this time, the sample (S) may exist in a diluted state by the diluent in the container (5).
  • the lower part of the container 5 is penetrated by the pin 183 of the through hole arranging member 180, and the sample S existing inside the container 5 is passed through the through hole 172. leaked into the
  • the lower part of the through hole 172 is formed on the lower sidewall of the cartridge insertion hole 120, that is, the lower cartridge insertion hole forming part 120b of the second part 110b of the main body 110. Since the state is closed by, the sample (S) can be accommodated inside the through hole (172).
  • the cartridge body 171 blocks the first and second reservoirs 140a and 140b and the discharge passage 160 .
  • the first pretreatment solution may be a solution that removes the infectivity of the virus contained in the sample and preserves the internal nucleic acid (eg, RNA) of the virus.
  • the second pretreatment solution may be a solution that dissolves the virus and removes a degrading enzyme (eg, RNA degrading enzyme) that degrades nucleic acids inside the virus.
  • the cartridge 170 is slidably displaced from the first position to the second position (Fig. 3(c)).
  • the through hole 172 of the cartridge 170 opens the lower portions of the first reservoir 140a and the second reservoir 140b, and the first reservoir 140a and the second reservoir 140b are opened. This is a position where it communicates with the discharge passage 160.
  • the through hole 172 of the cartridge is disposed below the first reservoir 140a and the second reservoir 140b, and the first reservoir The first communication part 142a and the second communication part 142b of the bottom 140a and the second reservoir 140b are opened.
  • the first reservoir 140a and the second reservoir 140b communicate with the discharge passage 160 through the through hole 172 .
  • the first pretreatment solution (S1) stored in the first reservoir (140a) and the second pretreatment solution (S2) stored in the second reservoir (140b) enter the through hole 172 and mix with the sample (S). and the mixed solution (S+S1+S2) of the sample, the first pretreatment solution, and the second pretreatment solution is discharged to the outside through the discharge passage 160.
  • the through hole 172 communicates with the sample inlet 130, and the lower part of the through hole 172 is formed by the lower sidewall of the cartridge insertion hole 120. It is closed, and the lower part of the one or more reservoirs 140a and 140b is closed by the cartridge body 171, so that the biomarker molecular diagnostic sample S enters the through hole 172 through the sample inlet 130. It is possible, and a pretreatment solution for pretreatment of a sample may be stored in one or more reservoirs 140a and 140b, respectively.
  • the through hole 172 is in communication with the one or more reservoirs 140a and 140b and the discharge passage 160 so that the one or more reservoirs 140a and 140b communicate with each other.
  • the pretreatment solution stored in (140a, 140b) and the sample may be mixed and flow into the discharge channel 160.
  • the sample discharged to the outside through the discharge channel 160 may be transferred to a biomarker molecular diagnosis device such as a PCR device.

Abstract

A virus pre-processing device is disclosed. A virus pre-processing device according to an embodiment of the present invention may comprise: a main body; a cartridge insertion port formed inwardly from one side of the main body; a sample input port which penetrates an upper surface of the main body and an upper sidewall of the cartridge insertion port; one or more reservoirs disposed on the top of the cartridge insertion port so as to be spaced apart from the sample input port, and wherein the lower portion thereof communicates with the cartridge insertion port; a discharge passage disposed below the cartridge insertion port so as to be spaced apart downwardly from the one or more reservoirs, and wherein the upper side thereof communicates with the cartridge insertion port; and a cartridge having a cartridge body which is slidably inserted into the cartridge insertion port, and a through hole which vertically penetrates the cartridge body.

Description

바이러스 전처리 장치Virus preprocessor
본 발명은 바이러스 전처리 장치에 관한 것으로, 보다 상세하게는 PCR(Polymerase Chain Reaction) 등과 같은 바이오마커 분자진단의 대상이 되는 바이러스 샘플의 전처리를 수행하는 바이러스 전처리 장치에 관한 것이다.The present invention relates to a virus pre-processing device, and more particularly, to a virus pre-processing device that performs pre-processing of a virus sample to be subjected to biomarker molecular diagnosis such as PCR (Polymerase Chain Reaction).
최근 2년 이상 코로나 바이러스 감염증(COVID-19)의 팬데믹 상황이 지속되고 있다. 또한, 전문가들은 코로나 바이러스 감영증 외에도 향후 다양한 종류의 바이러스 감염증의 대규모 유행이 발생할 수도 있음을 경고하고 있다. 코로나 바이러스 감염증을 비롯한 각종 바이러스 감염증의 대규모 유행을 최대한 억제하기 위해서는 증상 또는 무증상 감염자를 최대한 신속하게 많이 식별하여 격리시키는 것이 효과적이다.The pandemic situation of coronavirus infection (COVID-19) has been continuing for more than two years in recent years. In addition, experts are warning that a large-scale epidemic of various types of viral infections may occur in the future in addition to corona virus infections. In order to suppress the large-scale epidemic of various viral infections, including corona virus infections, as much as possible, it is effective to identify and isolate as many symptomatic or asymptomatic infected people as quickly as possible.
중합효소연쇄반응(PCR)을 기반으로 하는 핵산 증폭 테스트(Nucleic-acid amplification test, NAAT)는 바이러스 검출에 있어서 높은 분석 정확도(~99%)를 가지고 있어 각종 바이러스 감염증의 진단에 효과적인 수단으로 알려져 있다. 이로 인하여 코로나 바이러스 감염증의 진단에는 역전사 PCR(Reverse Transcription PCR, RT-PCR)이 표준으로 사용되고 있다.Polymerase chain reaction (PCR)-based nucleic acid amplification test (Nucleic-acid amplification test, NAAT) has high analytical accuracy (~99%) in virus detection and is known as an effective means for diagnosing various viral infections. . For this reason, reverse transcription PCR (RT-PCR) is used as a standard for diagnosis of coronavirus infection.
그런데 핵산 증폭 테스트가 정확하게 이루어지기 위해서는 바이러스가 포함된 샘플에 대한 전처리가 필요하다. 더욱 상세하게, 안전하고 정확한 진단을 위해서는 샘플에 존재하는 바이러스의 감염력을 제거하면서도 바이러스 내부의 핵산을 보존해야 한다. 그러나 샘플의 전처리는 진단을 수행하는 인력에 의해 이루어지는 것이 일반적이며, 샘플의 전처리 과정에서 샘플의 오염 등이 발생하기 쉽다. 한편, 샘플의 전처리를 자동으로 수행하는 장치들이 선보이기도 하였으나 샘플이 복수의 전처리 단계를 위해 수차례 이송되거나 반대로 복수개의 전처리 용액들이 샘플에 각각 이송되어야 하는 구조를 가지고 있어 구조가 복잡하고, 효율성이 낮은 문제를 가지고 있다.However, in order for the nucleic acid amplification test to be performed accurately, pretreatment is required for samples containing viruses. More specifically, for safe and accurate diagnosis, it is necessary to preserve the nucleic acid inside the virus while removing the infectivity of the virus present in the sample. However, pretreatment of the sample is generally performed by personnel performing the diagnosis, and contamination of the sample is likely to occur during the pretreatment of the sample. On the other hand, devices that automatically perform sample pretreatment have been introduced, but the structure is complicated and the efficiency is low because the sample has a structure in which the sample is transferred several times for a plurality of pretreatment steps or, conversely, a plurality of pretreatment solutions are transferred to the sample respectively. I have a low problem.
본 발명은 상기와 같은 문제점을 해결하기 위한 것으로, 본 발명의 목적은 바이러스를 포함하는 샘플의 전처리가 장치의 내부에서 수행되어 샘플의 오염 등의 문제를 최소화해주는 바이러스 전처리 장치를 제공하는 것이다.The present invention has been made to solve the above problems, and an object of the present invention is to provide a virus pretreatment device that minimizes problems such as sample contamination by performing pretreatment of samples containing viruses inside the device.
본 발명의 다른 목적은 간단한 작동을 통해 샘플의 전처리를 효율적으로 수행하는 바이러스 전처리 장치를 제공하는 것이다.Another object of the present invention is to provide a virus pre-processing device that efficiently performs sample pre-processing through a simple operation.
본 발명의 과제들은 이상에서 언급한 과제들로 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 본 발명이 속하는 기술분야의 통상의 기술자에게 명확하게 이해될 수 있을 것이다.The tasks of the present invention are not limited to the tasks mentioned above, and other tasks not mentioned will be clearly understood by those skilled in the art from the description below.
본 발명의 일 측면에 따르면, 본체; 상기 본체의 일측부에서 내측으로 형성된 카트리지 삽입구; 상기 본체의 상부면과 상기 카트리지 삽입구의 상부 측벽을 관통하는 샘플 투입구; 상기 카트리지 삽입구의 상부에 상기 샘플 투입구와 이격되어 배치되고, 하부는 상기 카트리지 삽입구와 연통되는 하나 이상의 리저버; 상기 하나 이상의 리저버의 하측으로 이격되어 상기 카트리지 삽입구의 하부에 배치되고 상측은 상기 카트리지 삽입구와 연통되는 배출 유로; 및 상기 카트리지 삽입구에 슬라이딩 가능하게 삽입되는 카트리지 몸체와, 상기 카트리지 몸체를 상하로 관통하는 관통홀을 가지는 카트리지;를 포함하는 바이러스 전처리 장치가 제공된다.According to one aspect of the invention, the body; a cartridge insertion port formed inwardly from one side of the main body; a sample inlet passing through an upper surface of the main body and an upper sidewall of the cartridge insertion port; one or more reservoirs disposed at an upper portion of the cartridge insertion port and spaced apart from the sample input port, and having a lower portion communicating with the cartridge insertion port; a discharge passage spaced apart from the lower side of the one or more reservoirs, disposed below the cartridge insertion port, and communicating with the upper side of the cartridge insertion port; and a cartridge having a cartridge body slidably inserted into the cartridge insertion hole and a through hole vertically penetrating the cartridge body.
이때, 상기 카트리지가 제 1 위치에 있을 때, 상기 관통홀은 상기 샘플 투입구와 연통되고, 상기 관통홀의 하부는 상기 카트리지 삽입구의 하부 측벽에 의해 폐쇄되며, 상기 하나 이상의 리저버의 하부는 상기 카트리지 몸체에 의해 폐쇄된 상태가 되어, 바이오마커 분자진단 샘플이 상기 샘플 투입구를 통해 상기 관통홀로 진입가능하고, 상기 하나 이상의 리저버에는 각각 상기 샘플의 전처리를 위한 전처리 용액이 저장될 수 있으며, 상기 카트리지가 상기 제 1 위치에서 슬라이딩된 제 2 위치에 있을 때, 상기 관통홀은 상기 하나 이상의 리저버 및 상기 배출 유로와 연통된 상태가 되어 상기 하나 이상의 리저버에 저장된 전처리 용액 및 상기 샘플이 혼합되어 상기 배출 유로로 유동할 수 있다.At this time, when the cartridge is in the first position, the through hole communicates with the sample inlet, the lower part of the through hole is closed by the lower side wall of the cartridge insertion hole, and the lower part of the one or more reservoirs is attached to the cartridge body. in a closed state, allowing a biomarker molecular diagnostics sample to enter the through hole through the sample inlet, a pretreatment solution for pretreatment of the sample may be stored in each of the one or more reservoirs, and the cartridge may enter the through hole. When in the second position slid from the first position, the through hole is in communication with the one or more reservoirs and the discharge passage so that the pretreatment solution stored in the one or more reservoirs and the sample are mixed and flow into the discharge passage. can
또한, 상기 카트리지 몸체는 사각 막대 형상을 가질 수 있다.In addition, the cartridge body may have a square rod shape.
또한, 상기 하나 이상의 리저버는, 제 1 전처리 용액을 저장할 수 있는 제 1 리저버; 및 제 2 전처리 용액을 저장할 수 있는 제 2 리저버;를 포함할 수 있다.In addition, the one or more reservoirs may include a first reservoir capable of storing a first pretreatment solution; and a second reservoir capable of storing the second pretreatment solution.
또한, 상기 제 1 리저버와 상기 제 2 리저버는 상기 카트리지의 슬라이딩 방향과 수직한 방향을 따라 나란히 배치될 수 있다.In addition, the first reservoir and the second reservoir may be disposed side by side along a direction perpendicular to the sliding direction of the cartridge.
또한, 상기 바이러스 전처리 장치는, 상기 관통홀의 내부에 고정되어 상하로 관통된 유로를 제공하며 상기 샘플을 담지한 용기가 상기 관통홀의 내부로 진입할 때, 상기 용기의 하부를 관통할 수 있는 핀부를 가지는 관통홀 배치부재를 더 포함할 수 있다.In addition, the virus preprocessing device is fixed to the inside of the through-hole to provide a flow path passing vertically, and a pin unit capable of penetrating the lower part of the container when the container carrying the sample enters the inside of the through-hole. The branch may further include a through-hole arranging member.
또한, 상기 하나 이상의 리저버는 상부가 개방되고, 상기 바이러스 전처리 장치는 상기 하나 이상의 리저버의 상부를 커버하는 하나 이상의 리저버 커버를 더 포함할 수 있다.In addition, the one or more reservoirs may have open tops, and the virus preprocessing device may further include one or more reservoir covers covering upper portions of the one or more reservoirs.
또한, 상기 본체는, 상기 샘플 투입구 및 상기 하나 이상의 리저버가 배치되고, 하측에 하부가 개방된 형태의 상부 카트리지 삽입구 형성부를 가지는 제 1 부분과, 상기 제 1 부분의 하측에 배치되고, 상기 배출 유로가 배치되며, 상기 제 1 부분과 결합된 상태에서 상기 상부 카트리지 삽입구 형성부의 개방된 하부를 폐쇄하며 상기 카트리지 삽입구를 형성하는 하부 카트리지 삽입구 형성부를 가지는 제 2 부분을 포함할 수 있다.In addition, the main body, the sample inlet and the one or more reservoirs are disposed, a first part having an upper cartridge insertion hole forming part having an open lower part, disposed below the first part, and the discharge passage Is disposed, may include a second part having a lower cartridge insertion hole forming portion to close the open lower portion of the upper cartridge insertion hole forming portion in a state coupled to the first portion and form the cartridge insertion hole.
상기의 구성에 따라 본 발명에 따른 바이러스 전처리 장치는 카트리지가 본체에 삽입되는 구조를 통해 샘플의 투입 및 샘플의 전처리를 위한 전처리 용액의 혼합이 장치의 내부에서 2단계 과정으로 완료될 수 있게 해주며, 샘플 전처리에 있어 높은 신뢰성 및 효율성을 제공한다.According to the above configuration, the virus pretreatment device according to the present invention allows the introduction of the sample and the mixing of the pretreatment solution for pretreatment of the sample to be completed in a two-step process inside the device through a structure in which the cartridge is inserted into the main body, , providing high reliability and efficiency in sample preparation.
본 발명의 효과는 상기한 효과로 한정되는 것은 아니며, 본 발명의 상세한 설명 또는 청구범위에 기재된 발명의 구성으로부터 추론 가능한 모든 효과를 포함하는 것으로 이해되어야 한다.The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be inferred from the detailed description of the present invention or the configuration of the invention described in the claims.
도 1은 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 사시도이다.1 is a perspective view of a virus pre-processing device according to an embodiment of the present invention.
도 2는 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 분해 사시도이다.2 is an exploded perspective view of a virus pre-processing device according to an embodiment of the present invention.
도 3은 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 작동을 나타낸 도면이다.3 is a diagram showing the operation of a virus pre-processing device according to an embodiment of the present invention.
도 4는 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 카트리지가 제 1 위치에 있을 때 샘플 투입구와 카트리지의 관통홀의 배치를 나타낸 단면도이다.4 is a cross-sectional view showing arrangement of a sample inlet and through-holes of the cartridge when the cartridge of the virus pre-processing device is in the first position according to an embodiment of the present invention.
도 5는 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 카트리지가 제 1 위치에 있을 때 하나 이상의 리저버와 카트리지의 배치를 나타낸 단면도이다.5 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the first position of the virus preprocessing device according to an embodiment of the present invention.
도 6은 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 카트리지가 제 2 위치에 있을 때 하나 이상의 리저버와 카트리지의 배치를 나타낸 단면도이다.6 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the second position of the virus preprocessing device according to an embodiment of the present invention.
이하, 첨부한 도면을 참고로 하여 본 발명의 실시예에 대하여 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 상세히 설명한다. 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며 여기에서 설명하는 실시예에 한정되지 않는다. 본 발명을 명확하게 설명하기 위해서 도면에서 설명과 관계없는 부분은 생략하였으며, 명세서 전체를 통하여 동일 또는 유사한 구성요소에 대해서는 동일한 참조부호를 붙였다.Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. This invention may be embodied in many different forms and is not limited to the embodiments set forth herein. In order to clearly describe the present invention, parts irrelevant to the description are omitted in the drawings, and the same reference numerals are assigned to the same or similar components throughout the specification.
본 명세서 및 청구범위에 사용된 단어와 용어는 통상적이거나 사전적인 의미로 한정 해석되지 않고, 자신의 발명을 최선의 방법으로 설명하기 위해 발명자가 용어와 개념을 정의할 수 있는 원칙에 따라 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야 한다. 본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 바람직한 일 실시예에 해당하고, 본 발명의 기술적 사상을 모두 대변하는 것이 아니므로 해당 구성은 본 발명의 출원시점에서 이를 대체할 다양한 균등물과 변형예가 있을 수 있다.Words and terms used in this specification and claims are not construed as limited in their ordinary or dictionary meanings, but in accordance with the principle that the inventors can define terms and concepts in order to best describe their inventions. It should be interpreted as a meaning and concept that corresponds to the technical idea. Since the embodiments described in this specification and the configurations shown in the drawings correspond to a preferred embodiment of the present invention and do not represent all of the technical ideas of the present invention, the corresponding configurations are various equivalents to replace them at the time of filing of the present invention. There may be water and variations.
본 명세서에서, "포함하다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성 요소, 부품 또는 이들을 조합한 것이 존재함을 설명하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성 요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.In this specification, terms such as "include" or "have" are intended to describe the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.
어떤 구성 요소가 다른 구성 요소의 "전방", "후방", "상부" 또는 "하부"에 있다는 것은 특별한 사정이 없는 한 다른 구성 요소와 바로 접하여 "전방", "후방", "상부" 또는 "하부"에 배치되는 것뿐만 아니라 그 중간에 또 다른 구성 요소가 배치되는 경우도 포함한다. 또한, 어떤 구성 요소가 다른 구성 요소와 "연결"되어 있다는 것은 특별한 사정이 없는 한 서로 직접 연결되는 것뿐만 아니라 간접적으로 서로 연결되는 경우도 포함한다.A component being in the "front", "rear", "above" or "below" of another component means that it is in direct contact with another component, unless there are special circumstances, and is "in front", "rear", "above" or "below". It includes not only those disposed at the lower part, but also cases in which another component is disposed in the middle. In addition, the fact that certain components are “connected” to other components includes cases where they are not only directly connected to each other but also indirectly connected to each other unless there are special circumstances.
도 1은 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 사시도이다. 또한, 도 2는 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 분해 사시도이다.1 is a perspective view of a virus pre-processing device according to an embodiment of the present invention. 2 is an exploded perspective view of a virus pre-processing device according to an embodiment of the present invention.
본 발명의 일 실시예에 따른 바이러스 전처리 장치(100)는 바이러스를 포함하는 샘플의 전처리를 수행한다. 예를 들면, 샘플은 PCR(Polymerase Chain Reaction)용 샘플이 될 수 있다. 더욱 상세하게, 상기 샘플은 PCR을 통한 핵산 검출의 대상이 되며, 피검자의 타액에서 RNA 또는 DNA를 정제하여 제조된 것일 수 있다. 이때, 검출 대상 핵산은 SARS-CoV-2 바이러스 검출을 위한 N1 및 N2 유전자와, 인간의 샘플임을 확인하기 위한 인간 RPP30 유전자가 될 수 있다. 물론, 이것은 하나의 예에 불과하며 진단하고자 하는 감염증의 종류에 따라 검출 대상 핵산은 달라질 수 있다.The virus pre-processing apparatus 100 according to an embodiment of the present invention performs pre-processing of a virus-containing sample. For example, the sample may be a sample for PCR (Polymerase Chain Reaction). More specifically, the sample is subject to nucleic acid detection through PCR, and may be prepared by purifying RNA or DNA from saliva of a subject. In this case, the nucleic acid to be detected may be the N1 and N2 genes for detecting the SARS-CoV-2 virus and the human RPP30 gene for confirming that the sample is a human sample. Of course, this is just one example, and the nucleic acid to be detected may vary depending on the type of infection to be diagnosed.
도 1 및 도 2를 참조하면, 본 발명의 일 실시예에 따른 바이러스 전처리 장치(100)는 본체(110), 카트리지 삽입구(120), 샘플 투입구(130), 하나 이상의 리저버(140a, 140b), 리저버 커버(150), 배출 유로(160), 카트리지(170) 및 관통홀 배치부재(180)를 포함할 수 있다.1 and 2, the virus preprocessing device 100 according to an embodiment of the present invention includes a main body 110, a cartridge insertion port 120, a sample inlet 130, one or more reservoirs 140a and 140b, It may include a reservoir cover 150, a discharge passage 160, a cartridge 170, and a through hole arranging member 180.
본체(110)는 샘플의 투입 및 이송을 위한 카트리지(170)가 삽입되고, 샘플의 전처리를 위한 전처리 용액의 저장을 위한 구성 및 전처리된 샘플의 배출을 위한 구성 등이 배치되도록 제공된다. 예를 들면, 본체(110)는 사각 박스 형태를 가질 수 있다.The main body 110 is provided so that the cartridge 170 for inputting and transporting the sample is inserted, and components for storing the pretreatment solution for pretreatment of the sample and components for discharging the pretreated sample are disposed. For example, the main body 110 may have a rectangular box shape.
본 발명의 일 실시예에서, 본체(110)는 샘플 투입구(130) 및 상기 하나 이상의 리저버(140a, 140b)가 배치되고, 하측에 하부가 개방된 형태의 상부 카트리지 삽입구 형성부(120a)를 가지는 제 1 부분(110a)과, 제 1 부분(110a)의 하측에 배치되고, 배출 유로(160)가 배치되며, 제 1 부분(110a)과 결합된 상태에서 상부 카트리지 삽입구 형성부(120a)의 개방된 하부를 폐쇄하며 카트리지 삽입구(120)를 형성하는 하부 카트리지 삽입구 형성부(120b)를 가지는 제 2 부분(110b)을 포함할 수 있다.In one embodiment of the present invention, the main body 110 has a sample inlet 130 and the one or more reservoirs 140a and 140b disposed, and has an upper cartridge insertion port forming portion 120a with an open bottom at the lower side. The first part 110a and the lower side of the first part 110a, the discharge passage 160 is disposed, and the opening of the upper cartridge insertion port forming part 120a in a state coupled with the first part 110a. It may include a second part (110b) having a lower cartridge insertion hole forming portion (120b) closing the lower portion and forming the cartridge insertion hole (120).
카트리지 삽입구(120)는 본체(110)의 일측부에서 내측으로 형성된다. 카트리지 삽입구(120)에는 카트리지(170)가 삽입된다. 뒤에서 상세하게 설명되는 바와 같이, 카트리지 삽입구(120)의 내부에서 카트리지(170)는 제 1 위치와 제 2 위치 사이를 슬라이딩 변위할 수 있다. 본 발명의 일 실시예에서, 카트리지 삽입구(120)는 본체(110)의 제 1 부분(110a)과 제 2 부분(110b)이 결합되어 본체(110)를 구성할 때, 본체(110)의 제 1 부분(110a)의 상부 카트리지 삽입구 형성부(120a)와 본체(110)의 제 2 부분(110b)의 하부 카트리지 삽입구 형성부(120b)에 의해 형성될 수 있다. Cartridge insertion port 120 is formed inward from one side of the main body 110. A cartridge 170 is inserted into the cartridge insertion port 120 . As described in detail later, the cartridge 170 can be slidably displaced between the first position and the second position inside the cartridge insertion port 120 . In one embodiment of the present invention, the cartridge insertion hole 120 is the first part (110a) and the second part (110b) of the main body 110 are combined to form the main body 110, the first part of the main body 110 It may be formed by the upper cartridge insertion hole forming part 120a of the first part 110a and the lower cartridge insertion hole forming part 120b of the second part 110b of the main body 110.
샘플 투입구(130)는 본체(110)의 상부면과 카트리지 삽입구(120)의 상부 측벽을 관통한다. 샘플 투입구(130)는 본체(110)의 일측에서 카트리지 진입 방향을 따라 소정 간격 이격된 지점의 상부에 형성될 수 있다. 본 발명의 일 실시예에서, 샘플 투입구(130)는 상하로 연장되는 실린더 형태를 가질 수 있다. The sample inlet 130 passes through the top surface of the main body 110 and the upper sidewall of the cartridge insertion hole 120 . The sample inlet 130 may be formed on one side of the main body 110 at an upper portion spaced apart by a predetermined interval along the cartridge entry direction. In one embodiment of the present invention, the sample inlet 130 may have a cylindrical shape extending up and down.
예를 들면, 샘플은 피검자의 타액이 희석액과 혼합된 형태로 마련될 수 있으며, 샘플 투입구(130)를 통해 피검자의 타액과 희석액이 혼합되어 담겨있는 용기가 투입될 수 있다. 한편, 샘플 투입구(130)를 통해 타액 등의 샘플이 직접 투입되는 것도 고려될 수 있다.For example, the sample may be prepared in the form of a mixture of the subject's saliva and the dilution liquid, and a container containing the mixture of the subject's saliva and the dilution liquid may be introduced through the sample inlet 130 . Meanwhile, it may also be considered that a sample such as saliva is directly introduced through the sample inlet 130 .
하나 이상의 리저버(140a, 140b)는 카트리지 삽입구(120)의 상부에 샘플 투입구(130)와 이격되어 배치되고, 하부는 카트리지 삽입구(120)와 연통된다. 하나 이상의 리저버(140a, 140b)는 샘플 투입구(130)에서 카트리지의 진입 방향을 따라 소정 간격 이격된 지점에 배치될 수 있다.One or more reservoirs (140a, 140b) is disposed spaced apart from the sample inlet 130 on the upper portion of the cartridge insertion port 120, the lower portion communicates with the cartridge insertion port (120). One or more reservoirs (140a, 140b) may be disposed at points spaced apart by a predetermined interval along the entry direction of the cartridge in the sample inlet (130).
하나 이상의 리저버는 제 1 리저버(140a) 및 제 2 리저버(140b)를 포함할 수 있다. 제 1 리저버(140a)는 카트리지(170)가 제 1 위치에 있을 때, 제 1 전처리 용액을 저장할 수 있다. 또한, 제 2 리저버(140b)는 카트리지(170)가 제 1 위치에 있을 때, 제 2 전처리 용액을 저장할 수 있다. 예를 들면, 제 1 전처리 용액은 샘플에 포함된 바이러스의 감염력을 제거하고, 바이러스의 내부 핵산(예를 들면, RNA)를 보존시키는 용액이 될 수 있다. 또한, 제 2 전처리 용액은 바이러스를 용해시키고, 바이러스 내부의 핵산을 분해하는 분해 효소(예를 들면, RNA 분해 효소)를 제거하는 용액이 될 수 있다. 관련하여, 제 2 전처리 용액은 염(salt)을 포함할 수 있다.One or more reservoirs may include a first reservoir 140a and a second reservoir 140b. The first reservoir 140a may store the first pretreatment solution when the cartridge 170 is in the first position. Also, the second reservoir 140b may store the second pretreatment solution when the cartridge 170 is in the first position. For example, the first pretreatment solution may be a solution that removes the infectivity of the virus included in the sample and preserves the internal nucleic acid (eg, RNA) of the virus. In addition, the second pretreatment solution may be a solution that dissolves the virus and removes a degrading enzyme (eg, RNA degrading enzyme) that degrades nucleic acids inside the virus. In this regard, the second pretreatment solution may include salt.
본 발명의 일 실시예에서, 제 1 리저버(140a)는 제 1 전처리 용액이 저장될 수 있는 제 1 저장부(141a)와, 제 1 저장부(141a)를 카트리지 삽입구(120)와 연통시키며 제 1 저장부(141a)의 하부에서 카트리지 삽입구(120)의 상부 측벽으로 연장된 제 1 연통부(142a)를 포함한다. 또한, 제 2 리저버(140b)는 제 2 전처리 용액이 저장될 수 있는 제 2 저장부(141b)와, 제 2 저장부(141b)를 카트리지 삽입구(120)와 연통시키며 제 2 저장부(141b)의 하부에서 카트리지 삽입구(120)의 상부 측벽으로 연장된 제 2 연통부(142b)를 포함한다. 이때, 제 1 연통부(142a) 및 제 2 연통부(142b)는 각각 제 1 저장부(141a)와 제 2 저장부(141b)에 비하여 좁게 형성될 수 있다. 제 1 연통부(142a) 및 제 2 연통부(142b)는 후술되는 카트리지의 카트리지 몸체(171)에 의해 폐쇄될 수 있으며, 제 1 연통부(142a) 및 제 2 연통부(142b)가 카트리지의 카트리지 몸체(171)에 의해 폐쇄된 상태에서 제 1 리저버(140a) 및 제 2 리저버(140b)에 제 1 전처리 용액 및 제 2 전처리 용액이 각각 저장될 수 있다.In one embodiment of the present invention, the first reservoir 140a communicates with the first reservoir 141a in which the first pretreatment solution can be stored and the first reservoir 141a communicates with the cartridge insertion port 120, and 1 includes a first communication part 142a extending from the lower part of the storage part 141a to the upper side wall of the cartridge insertion port 120. In addition, the second reservoir 140b communicates with the second storage unit 141b in which the second pretreatment solution can be stored and the second storage unit 141b communicates with the cartridge insertion port 120, and the second storage unit 141b It includes a second communication portion (142b) extending from the lower part to the upper sidewall of the cartridge insertion hole (120). In this case, the first communication portion 142a and the second communication portion 142b may be formed to be narrower than the first storage portion 141a and the second storage portion 141b, respectively. The first communication portion 142a and the second communication portion 142b may be closed by a cartridge body 171 of a cartridge to be described later, and the first communication portion 142a and the second communication portion 142b are connected to each other of the cartridge. A first pretreatment solution and a second pretreatment solution may be respectively stored in the first reservoir 140a and the second reservoir 140b in a closed state by the cartridge body 171 .
본 발명의 일 실시예에서, 제 1 리저버(140a)와 제 2 리저버(140b)는 나란히 배치될 수 있다. 더욱 상세하게, 제 1 리저버(140a)와 제 2 리저버(140b)는 카트리지의 진입 방향과 수직한 방향을 따라 나란하게 정렬되어 배치될 수 있다. 다시 말하면, 제 1 리저버(140a)와 제 2 리저버(140b)는 샘플 투입구(130)로부터 카트리지의 진입 방향을 따라 소정 간격 이격된 지점에 배치되되, 카트리지의 진입 방향과 수직한 방향을 따라 나란하게 정렬되어 배치될 수 있다.In one embodiment of the present invention, the first reservoir 140a and the second reservoir 140b may be disposed side by side. More specifically, the first reservoir 140a and the second reservoir 140b may be arranged side by side in a direction perpendicular to the entry direction of the cartridge. In other words, the first reservoir 140a and the second reservoir 140b are disposed at points spaced apart from the sample inlet 130 by a predetermined interval along the entry direction of the cartridge, and are parallel to the entry direction of the cartridge and perpendicular to the direction. They can be sorted and placed.
한편, 하나 이상의 리저버는 상부가 개방될 수 있다. 즉, 제 1 리저버(140a) 및 제 2 리저버(140b)는 각각 상부가 개방된 형태로 형성될 수 있다. 이러한 개방 구조는 제 1 전처리 용액 및 제 2 전처리 용액을 제 1 리저버(140a) 및 제 2 리버저(140b)의 세척 등에 유리한 효과를 제공한다.Meanwhile, one or more reservoirs may have an open top. That is, each of the first reservoir 140a and the second reservoir 140b may be formed with an open top. Such an open structure provides an advantageous effect such as washing the first reservoir 140a and the second reservoir 140b with the first pretreatment solution and the second pretreatment solution.
리저버 커버(150)는 하나 이상의 리저버(140a, 140b)의 상부를 커버한다. 하나 이상의 리저버에 각각 전처리 용액이 채워진 경우 전처리 용액의 오염 등을 방지하기 위해 하나 이상의 리저버의 개방된 상부는 폐쇄되는 것이 바람직하다. 리저버 커버(150)는 하나 이상의 리저버에 전처리 용액이 채워진 상태에서 하나 이상의 리저버의 개방된 상부를 폐쇄한다.The reservoir cover 150 covers the top of one or more reservoirs 140a and 140b. When each of the one or more reservoirs is filled with the pretreatment solution, the open top of the one or more reservoirs is preferably closed to prevent contamination of the pretreatment solution. The reservoir cover 150 closes the open top of one or more reservoirs while the pretreatment solution is filled in the one or more reservoirs.
본 발명의 일 실시예에서, 리저버 커버(150)는 제 1 리저버(140a) 및 제 2 리저버(140b)를 모두 커버한다. 각각의 리저버 별로 별개의 리저버 커버가 마련될 수도 있으나 본 발명의 일 실시예와 같이 하나의 리저버 커버(150)에 의해 복수의 리저버가 동시에 커버될 경우 리저버 커버(150)의 배치 시 효율성이 향상된다. 더욱 상세하게, 리저버 커버(150)는 제 1 리저버(140a) 및 제 2 리저버(140b)의 개방된 상태부를 커버하는 커버 몸체(151)를 포함하며, 커버 몸체(151)에는 각각 제 1 리저버(140a) 및 제 2 리저버(140b) 내부의 압력의 조절을 위한 제 1 벤트홀(152a) 및 제 2 벤트홀(152b)이 커버 몸체(151)를 관통하여 형성될 수 있다.In one embodiment of the present invention, the reservoir cover 150 covers both the first reservoir 140a and the second reservoir 140b. A separate reservoir cover may be provided for each reservoir, but as in one embodiment of the present invention, when a plurality of reservoirs are simultaneously covered by one reservoir cover 150, efficiency is improved when the reservoir cover 150 is placed. . More specifically, the reservoir cover 150 includes a cover body 151 covering the open state portions of the first reservoir 140a and the second reservoir 140b, and the cover body 151 includes the first reservoir ( 140a) and the second reservoir 140b, the first vent hole 152a and the second vent hole 152b for controlling internal pressure may be formed through the cover body 151.
배출 유로(160)는 하나 이상의 리저버(140a, 140b)의 하측으로 이격되어 카트리지 삽입구(120)의 하부에 배치되고 상측은 카트리지 삽입구(120)와 연통된다. 배출 유로(160)는 카트리지(170)의 카트리지 몸체(171)에 의해 폐쇄되어 하나 이상의 리저버(140a, 140b)와 연통이 차단된 상태가 될 수도 있고, 카트리지(170)의 관통홀(172)을 통해 하나 이상의 리저버(140a, 140b)와 연통된 상태가 될 수도 있다.The discharge flow passage 160 is spaced apart from the lower side of one or more reservoirs 140a and 140b and is disposed below the cartridge insertion port 120 and communicates with the upper side of the cartridge insertion port 120 . The discharge passage 160 may be closed by the cartridge body 171 of the cartridge 170 so that communication with one or more reservoirs 140a and 140b is blocked, and the through hole 172 of the cartridge 170 may be blocked. Through this, it may be in a state of communication with one or more reservoirs 140a and 140b.
배출 유로(160)가 카트리지(170)의 관통홀(172)을 통해 하나 이상의 리저버(140a, 140b)와 연통된 상태에서 전처리 용액과 혼합된 샘플이 배출 유로(160)를 통해 외부로 배출될 수 있다. 예를 들면, 외부로 배출된 샘플은 PCR 장치로 이송될 수 있다.In a state in which the discharge passage 160 communicates with one or more reservoirs 140a and 140b through the through hole 172 of the cartridge 170, the sample mixed with the pretreatment solution may be discharged to the outside through the discharge passage 160. there is. For example, the sample discharged to the outside may be transferred to a PCR device.
본 발명의 일 실시예에서, 배출 유로(160)는 카트리지 삽입구(120)와 연통되고, 상하 방향으로 연장되는 제 1 유로부(161)와, 제 1 유로부(161)와 외부를 연통시키며 측방으로 연장되는 제 2 유로부(162)를 포함할 수 있다. 이때, 제 1 유로부(161)의 단면적이 제 2 유로부(162)의 단면적보다 크게 형성될 수 있다. 이러한 구조는 전처리 용액과 혼합된 샘플이 외부로 배출되도록 유동할 때, 압력과 속도 측면에서 유리한 효과를 제공한다.In one embodiment of the present invention, the discharge passage 160 communicates with the cartridge insertion port 120 and extends in the vertical direction with the first passage portion 161, the first passage portion 161 and the outside communicate with the side It may include a second flow path portion 162 extending to . In this case, the cross-sectional area of the first flow path portion 161 may be larger than the cross-sectional area of the second flow path portion 162 . This structure provides advantageous effects in terms of pressure and speed when the sample mixed with the pretreatment solution flows to be discharged to the outside.
카트리지(170)는 카트리지 삽입구(120)에 슬라이딩 가능하게 삽입된다. 카트리지(170)는 카트리지 삽입구(120)에 슬라이딩 가능하게 삽입되는 카트리지 몸체(171)와, 카트리지 몸체(171)를 상하로 관통하는 관통홀(172)을 포함한다. 이때, 카트리지 몸체(171)는 사각 막대 형상을 가질 수 있다.The cartridge 170 is slidably inserted into the cartridge insertion port 120 . The cartridge 170 includes a cartridge body 171 slidably inserted into the cartridge insertion port 120 and a through hole 172 vertically penetrating the cartridge body 171 . At this time, the cartridge body 171 may have a square bar shape.
카트리지(170)는 투입구(130)를 통해 투입된 샘플을 수용하고, 샘플을 하나 이상의 리저버 측으로 이송한다. 또한, 카트리지(170)는 제 1 위치에서 카트리지 몸체(171)를 통해 하나 이상의 리저버(140a, 140b)의 하부를 폐쇄하여 전처리 용액이 저장 상태를 유지할 수 있게 해주며, 하나 이상의 리저버(140a, 140b)와 배출 유로(160)의 연통을 차단한다. 한편, 카트리지(170)는 제 2 위치에서 관통홀(172)을 통해 하나 이상의 리저버의 하부를 개방시키고 하나 이상의 리저버(140a, 140b)와 배출 유로(160)를 연통시켜 샘플과 전처리 용액이 혼합되어 배출 유로(160)를 통해 외부로 배출될 수 있게 해준다.The cartridge 170 receives the sample introduced through the inlet 130 and transfers the sample to one or more reservoirs. In addition, the cartridge 170 closes the lower part of the one or more reservoirs 140a and 140b through the cartridge body 171 in the first position so that the pretreatment solution can be maintained in a stored state, and the one or more reservoirs 140a and 140b ) and the discharge passage 160 are blocked. On the other hand, the cartridge 170 opens the lower part of one or more reservoirs through the through hole 172 at the second position and communicates the one or more reservoirs 140a and 140b with the discharge passage 160 so that the sample and the pretreatment solution are mixed. It allows to be discharged to the outside through the discharge passage (160).
관통홀 배치부재(180)는 카트리지(170)의 관통홀(172)에 배치되어, 샘플을 담지한 용기가 관통홀(172)의 내부로 진입할 때, 용기의 하부를 뚫어서 샘플이 관통홀(172)의 내부로 유출되도록 해준다. 본 발명의 일 실시예에서, 관통홀 배치부재(180)는 관통홀(172)의 내부에 고정 장착되는 배치 몸체부(181), 배치 몸체부(181)를 상하로 관통하여 유로를 제공하는 유로 형성부(182) 및 샘플을 담지한 용기가 관통홀(172)의 내부로 진입할 때, 상기 용기의 하부를 관통하도록 배치 몸체부(181)에 연결된 핀부(183)를 포함할 수 있다.The through-hole arranging member 180 is disposed in the through-hole 172 of the cartridge 170, and when a container carrying the sample enters the through-hole 172, the sample penetrates the lower portion of the container through the through-hole ( 172) to allow it to flow into the interior. In one embodiment of the present invention, the through-hole arranging member 180 is a placement body portion 181 fixedly mounted inside the through-hole 172, and a flow passage that vertically penetrates the placement body portion 181 to provide a flow path. When the formation part 182 and the container carrying the sample enter the through hole 172, the pin part 183 connected to the body part 181 may be included so as to pass through the lower part of the container.
도 3은 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 작동을 나타낸 도면이다. 도 4는 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 카트리지가 제 1 위치에 있을 때 샘플 투입구와 카트리지의 관통홀의 배치를 나타낸 단면도이고, 도 5는 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 카트리지가 제 1 위치에 있을 때 하나 이상의 리저버와 카트리지의 배치를 나타낸 단면도이다. 또한, 도 6은 본 발명의 일 실시예에 따른 바이러스 전처리 장치의 카트리지가 제 2 위치에 있을 때 하나 이상의 리저버와 카트리지의 배치를 나타낸 단면도이다.3 is a diagram showing the operation of a virus pre-processing device according to an embodiment of the present invention. 4 is a cross-sectional view showing the arrangement of a sample inlet and through-holes of the cartridge when the cartridge of the virus pre-processing device is in the first position according to an embodiment of the present invention, and FIG. 5 is a virus pre-processing device according to an embodiment of the present invention. A cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the first position. 6 is a cross-sectional view showing the arrangement of one or more reservoirs and cartridges when the cartridges are in the second position of the virus preprocessing device according to an embodiment of the present invention.
도 3 내지 도 6을 참조하여, 본 발명의 일 실시예에 따른 바이러스 전처리 장치(100)의 작동을 설명한다.3 to 6, the operation of the virus pre-processing device 100 according to an embodiment of the present invention will be described.
먼저, 카트리지(170)를 카트리지 삽입구(120)에 삽입하고(도 3의 (a)), 카트리지(170)를 제 1 위치에 배치한다(도 3의 (b)). 여기서, 제 1 위치는 카트리지(170)의 관통홀(172)이 본체(110)에 형성된 샘플 투입구(130)와 연통되고, 카트리지 몸체(171)가 제 1 리저버(140a) 및 제 2 리저버(140b)의 하부를 폐쇄하고, 제 1 리저버(140a) 및 제 2 리저버(140b)와 배출 유로(160) 사이의 연통을 차단하는 위치를 의미한다.First, the cartridge 170 is inserted into the cartridge insertion hole 120 (FIG. 3(a)), and the cartridge 170 is placed in a first position (FIG. 3(b)). Here, in the first position, the through hole 172 of the cartridge 170 communicates with the sample inlet 130 formed in the main body 110, and the cartridge body 171 is connected to the first reservoir 140a and the second reservoir 140b. It means a position where the lower part of ) is closed and communication between the first reservoir 140a and the second reservoir 140b and the discharge passage 160 is blocked.
도 4에 나타난 바와 같이, 카트리지(170)가 제 1 위치에 있을 때, 샘플(S)을 담지한 용기(5)가 샘플 투입구(130)를 통해 진입하여 카트리지(170)의 관통홀(172)에 도달할 수 있다. 이때, 샘플(S)은 용기(5) 내에서 희석액에 으해 희석된 상태로 존재할 수 있다. 관통홀(172)의 내부에서 관통홀 배치부재(180)의 핀부(183)에 의해 용기(5)의 하부가 관통되어 용기(5)의 내부에 존재하는 샘플(S)이 관통홀(172)의 내부로 유출된다. 카트리지(170)가 제 1 위치에 있을 때, 관통홀(172)의 하부는 카트리지 삽입구(120)의 하부 측벽 즉, 본체(110)의 제 2 부분(110b)의 하부 카트리지 삽입구 형성부(120b)에 의해 폐쇄된 상태이므로, 샘플(S)은 관통홀(172)의 내부에 수용될 수 있다.As shown in FIG. 4, when the cartridge 170 is in the first position, the container 5 holding the sample S enters through the sample inlet 130 and passes through the through hole 172 of the cartridge 170. can reach At this time, the sample (S) may exist in a diluted state by the diluent in the container (5). Inside the through hole 172, the lower part of the container 5 is penetrated by the pin 183 of the through hole arranging member 180, and the sample S existing inside the container 5 is passed through the through hole 172. leaked into the When the cartridge 170 is in the first position, the lower part of the through hole 172 is formed on the lower sidewall of the cartridge insertion hole 120, that is, the lower cartridge insertion hole forming part 120b of the second part 110b of the main body 110. Since the state is closed by, the sample (S) can be accommodated inside the through hole (172).
도 5에 나타난 바와 같이, 카트리지(170)가 제 1 위치에 있을 때, 제 1 리버저(140a) 및 제 2 리저버(140b)는 카트리지 몸체(171)에 의해 폐쇄되어 있다. 이에 따라 제 1 리버저(140a) 및 제 2 리저버(140b)에는 제 1 전처리 용액(S1)과 제 2 전처리 용액(S2)이 저장될 수 있다. 한편, 제 1 리버저(140a) 및 제 2 리저버(140b)와 배출 유로(160) 사이는 카트리지 몸체(171)에 의해 차단된다.As shown in FIG. 5 , when the cartridge 170 is in the first position, the first reservoir 140a and the second reservoir 140b are closed by the cartridge body 171 . Accordingly, the first pretreatment solution S1 and the second pretreatment solution S2 may be stored in the first reservoir 140a and the second reservoir 140b. Meanwhile, the cartridge body 171 blocks the first and second reservoirs 140a and 140b and the discharge passage 160 .
전술한 바와 같이, 제 1 전처리 용액은 샘플에 포함된 바이러스의 감염력을 제거하고, 바이러스의 내부 핵산(예를 들면, RNA)를 보존시키는 용액이 될 수 있다. 또한, 제 2 전처리 용액은 바이러스를 용해시키고, 바이러스 내부의 핵산을 분해하는 분해 효소(예를 들면, RNA 분해 효소)를 제거하는 용액이 될 수 있다.As described above, the first pretreatment solution may be a solution that removes the infectivity of the virus contained in the sample and preserves the internal nucleic acid (eg, RNA) of the virus. In addition, the second pretreatment solution may be a solution that dissolves the virus and removes a degrading enzyme (eg, RNA degrading enzyme) that degrades nucleic acids inside the virus.
다음으로, 카트리지(170)가 제 1 위치로부터 제 2 위치로 슬라이딩 변위한다(도 3의 (c)). 여기서, 제 2 위치는 카트리지(170)의 관통홀(172)이 제 1 리저버(140a) 및 제 2 리저버(140b)의 하부를 개방시키고, 제 1 리저버(140a) 및 제 2 리저버(140b)를 배출 유로(160)와 연통시키는 위치이다.Next, the cartridge 170 is slidably displaced from the first position to the second position (Fig. 3(c)). Here, in the second position, the through hole 172 of the cartridge 170 opens the lower portions of the first reservoir 140a and the second reservoir 140b, and the first reservoir 140a and the second reservoir 140b are opened. This is a position where it communicates with the discharge passage 160.
도 6에 나타난 바와 같이, 카트리지(170)가 제 2 위치에 있을 때, 제 1 리버저(140a) 및 제 2 리저버(140b)의 하측에 카트리지의 관통홀(172)이 배치되면서, 제 1 리버저(140a) 및 제 2 리저버(140b)의 제 1 연통부(142a) 및 제 2 연통부(142b)가 개방된다. 또한, 제 1 리버저(140a) 및 제 2 리저버(140b)는 관통홀(172)을 통해 배출 유로(160)와 연통된다. 이에 따라 제 1 리저버(140a)에 저장된 제 1 전처리 용액(S1) 및 제 2 리저버(140b)에 저장된 제 2 전처리 용액(S2)이 관통홀(172)의 내부로 진입하여 샘플(S)과 혼합되고, 샘플, 제 1 전처리 용액 및 제 2 전처리 용액의 혼합액(S+S1+S2)이 배출 유로(160)를 통해 외부로 배출된다.As shown in FIG. 6, when the cartridge 170 is in the second position, the through hole 172 of the cartridge is disposed below the first reservoir 140a and the second reservoir 140b, and the first reservoir The first communication part 142a and the second communication part 142b of the bottom 140a and the second reservoir 140b are opened. In addition, the first reservoir 140a and the second reservoir 140b communicate with the discharge passage 160 through the through hole 172 . Accordingly, the first pretreatment solution (S1) stored in the first reservoir (140a) and the second pretreatment solution (S2) stored in the second reservoir (140b) enter the through hole 172 and mix with the sample (S). and the mixed solution (S+S1+S2) of the sample, the first pretreatment solution, and the second pretreatment solution is discharged to the outside through the discharge passage 160.
이상 살펴본 바와 같이, 카트리지(170)가 제 1 위치에 있을 때, 관통홀(172)은 샘플 투입구(130)와 연통되고, 관통홀(172)의 하부는 카트리지 삽입구(120)의 하부 측벽에 의해 폐쇄되며, 하나 이상의 리저버(140a, 140b)의 하부는 카트리지 몸체(171)에 의해 폐쇄된 상태가 되어, 바이오마커 분자진단 샘플(S)이 샘플 투입구(130)를 통해 관통홀(172)로 진입가능하고, 하나 이상의 리저버(140a, 140b)에는 각각 샘플의 전처리를 위한 전처리 용액이 저장될 수 있다.As described above, when the cartridge 170 is in the first position, the through hole 172 communicates with the sample inlet 130, and the lower part of the through hole 172 is formed by the lower sidewall of the cartridge insertion hole 120. It is closed, and the lower part of the one or more reservoirs 140a and 140b is closed by the cartridge body 171, so that the biomarker molecular diagnostic sample S enters the through hole 172 through the sample inlet 130. It is possible, and a pretreatment solution for pretreatment of a sample may be stored in one or more reservoirs 140a and 140b, respectively.
또한, 카트리지(170)가 상기 제 1 위치에서 슬라이딩된 제 2 위치에 있을 때, 관통홀(172)은 하나 이상의 리저버(140a, 140b) 및 배출 유로(160)와 연통된 상태가 되어 하나 이상의 리저버(140a, 140b)에 저장된 전처리 용액 및 상기 샘플이 혼합되어 배출 유로(160)로 유동할 수 있다. 배출 유로(160)를 통해 외부로 배출된 샘플은 PCR 장치 등과 같은 바이오마커 분자진단 장치로 이송될 수 있다.In addition, when the cartridge 170 is in the second position slid from the first position, the through hole 172 is in communication with the one or more reservoirs 140a and 140b and the discharge passage 160 so that the one or more reservoirs 140a and 140b communicate with each other. The pretreatment solution stored in (140a, 140b) and the sample may be mixed and flow into the discharge channel 160. The sample discharged to the outside through the discharge channel 160 may be transferred to a biomarker molecular diagnosis device such as a PCR device.
본 발명의 실시예에 대하여 설명하였으나, 본 발명의 사상은 본 명세서에 제시되는 실시예에 의해 제한되지 아니하며, 본 발명의 사상을 이해하는 당업자는 동일한 사상의 범위 내에서, 구성요소의 부가, 변경, 삭제, 추가 등에 의해서 다른 실시예를 용이하게 제안할 수 있을 것이나, 이 또한 본 발명의 사상범위 내에 든다고 할 것이다.Although the embodiments of the present invention have been described, the spirit of the present invention is not limited by the embodiments presented herein, and those skilled in the art who understand the spirit of the present invention may add or change components within the scope of the same spirit. Other embodiments can be easily proposed by adding, deleting, adding, etc., but this will also be said to be within the scope of the present invention.

Claims (8)

  1. 본체;main body;
    상기 본체의 일측부에서 내측으로 형성된 카트리지 삽입구;a cartridge insertion port formed inwardly from one side of the main body;
    상기 본체의 상부면과 상기 카트리지 삽입구의 상부 측벽을 관통하는 샘플 투입구;a sample inlet passing through an upper surface of the main body and an upper sidewall of the cartridge insertion port;
    상기 카트리지 삽입구의 상부에 상기 샘플 투입구와 이격되어 배치되고, 하부는 상기 카트리지 삽입구와 연통되는 하나 이상의 리저버;one or more reservoirs disposed at an upper portion of the cartridge insertion port and spaced apart from the sample input port, and having a lower portion communicating with the cartridge insertion port;
    상기 하나 이상의 리저버의 하측으로 이격되어 상기 카트리지 삽입구의 하부에 배치되고 상측은 상기 카트리지 삽입구와 연통되는 배출 유로; 및a discharge passage spaced apart from the lower side of the one or more reservoirs, disposed below the cartridge insertion port, and communicating with the upper side of the cartridge insertion port; and
    상기 카트리지 삽입구에 슬라이딩 가능하게 삽입되는 카트리지 몸체와, 상기 카트리지 몸체를 상하로 관통하는 관통홀을 가지는 카트리지;를 포함하는 바이러스 전처리 장치.A virus preprocessing device comprising: a cartridge body slidably inserted into the cartridge insertion port, and a cartridge having a through hole vertically penetrating the cartridge body.
  2. 제 1 항에 있어서,According to claim 1,
    상기 카트리지가 제 1 위치에 있을 때, 상기 관통홀은 상기 샘플 투입구와 연통되고, 상기 관통홀의 하부는 상기 카트리지 삽입구의 하부 측벽에 의해 폐쇄되며, 상기 하나 이상의 리저버의 하부는 상기 카트리지 몸체에 의해 폐쇄된 상태가 되어, 바이오마커 분자진단 샘플이 상기 샘플 투입구를 통해 상기 관통홀로 진입가능하고, 상기 하나 이상의 리저버에는 각각 상기 샘플의 전처리를 위한 전처리 용액이 저장될 수 있으며,When the cartridge is in the first position, the through hole communicates with the sample inlet, the lower part of the through hole is closed by the lower side wall of the cartridge insertion hole, and the lower part of the one or more reservoirs is closed by the cartridge body. In this state, a biomarker molecular diagnostic sample can enter the through hole through the sample inlet, and a pretreatment solution for pretreatment of the sample can be stored in each of the one or more reservoirs,
    상기 카트리지가 상기 제 1 위치에서 슬라이딩된 제 2 위치에 있을 때, 상기 관통홀은 상기 하나 이상의 리저버 및 상기 배출 유로와 연통된 상태가 되어 상기 하나 이상의 리저버에 저장된 전처리 용액 및 상기 샘플이 혼합되어 상기 배출 유로로 유동할 수 있는 바이러스 전처리 장치.When the cartridge is in the second position slid from the first position, the through hole is in a state of communication with the one or more reservoirs and the discharge passage so that the pretreatment solution stored in the one or more reservoirs and the sample are mixed and the A virus pre-treatment device that can flow into the discharge flow path.
  3. 제 1 항에 있어서,According to claim 1,
    상기 카트리지 몸체는 사각 막대 형상을 가지는 바이러스 전처리 장치.The cartridge body is a virus pre-processing device having a square bar shape.
  4. 제 1 항에 있어서,According to claim 1,
    상기 하나 이상의 리저버는,The one or more reservoirs,
    제 1 전처리 용액을 저장할 수 있는 제 1 리저버; 및a first reservoir capable of storing a first pretreatment solution; and
    제 2 전처리 용액을 저장할 수 있는 제 2 리저버;를 포함하는 바이러스 전처리 장치.A virus pretreatment device comprising a; second reservoir capable of storing a second pretreatment solution.
  5. 제 4 항에 있어서,According to claim 4,
    상기 제 1 리저버와 상기 제 2 리저버는 상기 카트리지의 슬라이딩 방향과 수직한 방향을 따라 나란히 배치되는 바이러스 전처리 장치.The first reservoir and the second reservoir are arranged side by side along a direction perpendicular to the sliding direction of the cartridge.
  6. 제 1 항에 있어서,According to claim 1,
    상기 관통홀의 내부에 고정되어 상하로 관통된 유로를 제공하며 상기 샘플을 담지한 용기가 상기 관통홀의 내부로 진입할 때, 상기 용기의 하부를 관통할 수 있는 핀부를 가지는 관통홀 배치부재를 더 포함하는 바이러스 전처리 장치.Further comprising a through-hole arrangement member fixed to the inside of the through-hole to provide a flow path penetrating vertically and having a pin portion capable of penetrating the lower portion of the container when the container carrying the sample enters the inside of the through-hole. Virus pre-processing device.
  7. 제 1 항에 있어서,According to claim 1,
    상기 하나 이상의 리저버는 상부가 개방되고,The one or more reservoirs are open at the top,
    상기 하나 이상의 리저버의 상부를 커버하는 하나 이상의 리저버 커버를 더 포함하는 바이러스 전처리 장치.Virus pre-processing apparatus further comprising one or more reservoir covers covering an upper portion of the one or more reservoirs.
  8. 제 1 항에 있어서,According to claim 1,
    상기 본체는,the body,
    상기 샘플 투입구 및 상기 하나 이상의 리저버가 배치되고, 하측에 하부가 개방된 형태의 상부 카트리지 삽입구 형성부를 가지는 제 1 부분과,A first part in which the sample inlet and the one or more reservoirs are disposed and has an upper cartridge insertion hole formed at a lower side with an open lower part;
    상기 제 1 부분의 하측에 배치되고, 상기 배출 유로가 배치되며, 상기 제 1 부분과 결합된 상태에서 상기 상부 카트리지 삽입구 형성부의 개방된 하부를 폐쇄하며 상기 카트리지 삽입구를 형성하는 하부 카트리지 삽입구 형성부를 가지는 제 2 부분을 포함하는 바이러스 전처리 장치.Disposed on the lower side of the first part, the discharge flow passage is disposed, having a lower cartridge insertion hole formation portion for forming the cartridge insertion hole and closing the open lower portion of the upper cartridge insertion hole formation portion in a state coupled to the first portion A virus pre-processing device comprising a second part.
PCT/KR2022/018764 2021-12-29 2022-11-24 Virus pre-processing device WO2023128302A1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
KR10-2021-0191807 2021-12-29
KR10-2021-0191806 2021-12-29
KR20210191807 2021-12-29
KR20210191806 2021-12-29
KR10-2022-0155404 2022-11-18
KR1020220155404A KR20230101703A (en) 2021-12-29 2022-11-18 Device for preprocessing viruses

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4623716B2 (en) * 2004-11-25 2011-02-02 旭化成株式会社 Nucleic acid detection cartridge and nucleic acid detection method
KR101630784B1 (en) * 2014-09-24 2016-06-15 한국기계연구원 Catridge for sample preparation
KR20180125816A (en) * 2017-05-16 2018-11-26 에스케이텔레콤 주식회사 Apparatus for nucleic acid analysis using cartridge
KR101967236B1 (en) * 2017-05-16 2019-04-09 에스케이텔레콤 주식회사 Pretreatment chamber for nucleic acid extraction, cartridge and nucleic acid extraction method using the same
KR20200018475A (en) * 2017-06-21 2020-02-19 베링거잉겔하임베트메디카게엠베하 Compressible Extraction Apparatus for Pretreatment of Samples

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4623716B2 (en) * 2004-11-25 2011-02-02 旭化成株式会社 Nucleic acid detection cartridge and nucleic acid detection method
KR101630784B1 (en) * 2014-09-24 2016-06-15 한국기계연구원 Catridge for sample preparation
KR20180125816A (en) * 2017-05-16 2018-11-26 에스케이텔레콤 주식회사 Apparatus for nucleic acid analysis using cartridge
KR101967236B1 (en) * 2017-05-16 2019-04-09 에스케이텔레콤 주식회사 Pretreatment chamber for nucleic acid extraction, cartridge and nucleic acid extraction method using the same
KR20200018475A (en) * 2017-06-21 2020-02-19 베링거잉겔하임베트메디카게엠베하 Compressible Extraction Apparatus for Pretreatment of Samples

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