WO2023113399A1 - Antibacterial functional cosmetic composition for antioxidation, anti-inflammation and anti-aging comprising gardenia florida flower extract, pelargonium graveolens extract, and salvia officinalis extract as active ingredients - Google Patents

Abstract

The present invention relates to: an antibacterial functional cosmetic composition comprising, as active ingredients, a Gardenia Florida Flower extract, a Pelargonium Graveolens extract, a Salvia Officinalis extract, 3-0-ethyl ascorbic acid, and panthenol; and a method for preparing same. The cosmetic composition provided by the present invention is an antibacterial functional cosmetic composition which comprises natural ingredients as main ingredients, thus minimizing skin irritation, exhibits excellent antibacterial activity by effectively inhibiting the proliferation of bacteria, and also has excellent anti-inflammatory and antioxidant effects, as well as overall skin improvement effects.

Description

Antioxidant, anti-inflammatory, and anti-aging functional cosmetic composition containing Gardenia flower extract, Pelargonium graveolens extract, and Salvia officinalis extract as active ingredients

The present invention relates to an antibacterial functional cosmetic composition comprising gardenia flower extract, Pelargonium graveolens extract, and Salvia officinalis extract as active ingredients. It relates to an improved cosmetic composition and a manufacturing method thereof.

In order to promote hygiene and personal safety from disease, disinfection and virus removal, especially from the hands, is important. In the past, hand sanitizer was an essential element for people interested in hygiene or health other than some professional occupations, but recently, due to COVID-19, the importance of hand disinfection has become essential knowledge for the general public beyond common sense. If you keep your hands clean, you can prevent most infectious diseases as well as Corona 19.

As a result of a survey by the World Health Organization (WHO), it is reported that 40% of germs are sterilized when washed only with water, 20% when washed with general soap, and 99.99% when washed with alcohol-based hand sanitizer. However, even solid soap can remove about 90% of germs if you wash your hands for 15 seconds and 99% if you wash your hands for 30 seconds according to the WHO's hand hygiene guidelines. It should be noted that by breeding, there is a risk of becoming infected while washing hands or getting infected while rinsing with contaminated water. In addition, soap has the inconvenience of having to be washed with water after use, and a bigger disadvantage is that it uses a skin moisturizer, but it is used before the skin moisturizer, which should remain on the skin due to water washing and the action of the surfactant, functions properly. There are disadvantages of the prior art that are washed away. In particular, washing of bacteria or skin contamination in the absence of water was difficult.

Therefore, alcohol-containing disinfectants have been developed that can exert sterilization power even where there is no water, and the form is classified into a gel type and a liquid type. Looking at the patented technologies related to these alcohol-based disinfectants, Korean Patent Publication No. 10-2001-0044514 (published on June 5, 2001) contains 60-68% by volume of ethanol and 2-5% by volume of isopropanol, gel. "High bactericidal composition" containing 0.1-0.3% by weight of carbomer as a topical, and 30 to 80% by weight of ethanol in Korean Patent Registration No. 10-0425943 (published on April 3, 2004); 0.01 to 5% by weight of isopropyl alcohol; 0.01 to 1% by weight of benzalkonium chloride; 0.5 to 6% by weight of a skin moisturizer selected from the group consisting of propylene glycol, aloe vera, glycerin, sorbitol, isopropyl myristate, and silicone derivatives; And a "lotion-type skin protection disinfectant composition" characterized in that the remainder contains water. However, most of these alcohol-based disinfectants contain 60 to 85% of alcohol, and up to 95% of alcohol. Although these alcohol components can bring about excellent sterilization effects, even if a small amount of moisturizer is added as a component, alcohol Since the components are volatilized, the moisture and nutrients of the human body and hands to which the composition is applied are volatilized together, and the skin still becomes dry, tight, and crumbly.

In addition, since human skin, which has become sensitive due to the influence of environmental pollution, may be stimulated by alcohol components, consumers with sensitive skin prefer non-alcoholic products that do not contain alcohol components.

As people's interest in personal hygiene such as hand disinfection increases to prevent such infectious diseases and infections, various antibacterial compositions have been developed and manufactured as personal hygiene products, and other chemical additives are added to enhance moisturizing properties. . When the alcohol-based antibacterial cosmetic composition is applied to the skin, it dries again at an early point in time, and the moisturizing durability is not excellent, and skin irritation may occur due to the use of chemical additives.

Accordingly, the present inventors have developed a technology including natural ingredients as active ingredients, minimizing skin irritation, improving antibacterial function, and furthermore, including excellent anti-inflammatory and antioxidant functions, leading to the present invention.

The present invention relates to an antibacterial functional cosmetic composition comprising gardenia flower extract, Pelargonium graveolens extract, and Salvia officinalis extract as active ingredients.

An object of the present invention is to provide an antimicrobial functional cosmetic composition comprising a mixed extract of Gardenia flower extract, Pelargonium graveolens extract, and Salvia officinalis extract, and a method for preparing the same.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

According to one embodiment of the present invention, a mixed extract of Gardenia Florida Flower extract, Pelargonium Graveolens extract and Salvia Officinalis (Sage) extract and purified water Antibacterial functional cosmetic composition comprising is provided.

In the present invention, the gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract may be mixed in a weight ratio of 1:0.5 to 5:2 to 7 in a cosmetic composition.

In the present invention, the mixed extract may be a cosmetic composition further comprising at least one of 3-0-ethyl ascorbic acid and panthenol.

In the present invention, the mixed extract may be a cosmetic composition containing 0.001 to 40% by weight based on the total weight of the cosmetic composition.

In the present invention, the Gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid, and the panthenol are mixed in a ratio of 1:0.5 to 5:2 to 7:0.05. ~0.3: It may be a cosmetic composition mixed in a weight ratio of 0.1 to 0.8.

In the present invention, the cosmetic composition may be a cosmetic composition containing at least one selected from the group consisting of thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, and fragrances.

In the present invention, the cosmetic composition may be a cosmetic composition having anti-inflammatory, antioxidant, and anti-aging functions.

According to another embodiment of the present invention, gardenia flower ( Gardenia Florida Flower ), Pelargonium Graveolens ( Pelargonium Graveolens ) and Salvia Officinalis ( Salvia Officinalis (Sage)) raw materials are ultrasonically extracted to obtain extracts There is provided a method for producing an antimicrobial functional cosmetic composition comprising the step.

In the present invention, 3-0-ethyl ascorbic acid was added to the Gardenia Florida Flower extract, the Pelargonium Graveolens extract, and the Salvia Officinalis (Sage) extract. (3-0-Ethyl Ascorbic acid) and panthenol (Panthenol) may be a method for producing an antimicrobial functional cosmetic composition comprising the step of mixing any one or more.

The antibacterial functional cosmetic composition provided in the present invention has excellent antibacterial effect as well as anti-inflammatory, antioxidant and anti-aging effects, and contains natural ingredients such as gardenia flower extract, Pelargonium graveolens extract, and Salvia officinalis extract. It provides an antimicrobial functional cosmetic composition with excellent antioxidant and anti-inflammatory activity while minimizing skin irritation by using it as an active ingredient.

Hereinafter, an example will be described in detail for a more detailed explanation. However, the following examples are illustrative, and the scope of the present invention is not limited thereto.

According to one embodiment of the present invention, a mixed extract of Gardenia Florida Flower extract, Pelargonium Graveolens extract and Salvia Officinalis (Sage) extract and purified water It relates to an antimicrobial functional cosmetic composition comprising.

In the present invention, as the mixed extract, extracts obtained by drying and crushing Gardenia flower, Pelargonium graveolens, and Salvia officinalis and then pulverizing them may be used.

The gardenia flower ( Gardenia Florida Flower ) is a plant of the genus Gardenia, and the stem, root, leaf, flower, petal, seed (seed), fruit pulp, fruit peel or fruit of the gardenia flower may be crushed, cut, or dried, and these are alone It may be used as a mixture, or may be used in combination, and there is no particular limitation as long as it is commonly used as a gardenia flower containing a triterpenoid component as a type of plant. There are about 250 species of gardenia flowers all over the world, distributed in tropical and subtropical regions, and 5 species in China and 1 variant species can all be used as medicine, and the flowers contain triterpenoids and the like.

The Pelargonium Graveolens ( Pelargonium Graveolens ) is an aromatic plant of the Pelargonium genus with essential oils in leaves and stems, and the stem, root, leaf, flower, petal, seed (seed), and fruit of Pelargonium Graveolens , The fruit peel or fruit may be crushed, cut, or dried, and may be used alone or mixed, and as long as it is commonly used as Pelargonium graveolens as a type of plant, there is no particular limitation. The Pelargonium graveolens is distributed in a wide area such as South Africa, Europe, and Asia, and is cultivated a lot for herbs in Korea, and is mostly used as a fragrance for perfumes, cosmetics, soaps, and the like, and is also used as a medicine.

The Salvia Officinalis (Sage) is a plant of the genus Sage, and the stem, root, leaf, flower, petal, seed (seed), fruit pulp, fruit peel or fruit of Salvia Officinalis It may be crushed, cut, or dried, and may be used alone or in combination, and there is no particular limitation as long as it is commonly used as Salvia officinalis as a type of plant. The Salvia officinalis is also called medicinal sage, and it is known that boiled leaves are used for sore throat and gastroenteritis for medicinal purposes.

The mixed extract may further include a crude extract, a fraction, or a combination thereof of the Gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract.

The crude extract is obtained by contacting the Gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract with an extraction solvent, and is a mixture of various substances, and the fraction is specific for the crude extract. A material containing components is separated, and the separation may be separation using an organic solvent, chromatography, or filtration, and the chromatography may be, for example, silica gel column chromatography, Sephadex LH-20 chromatography, or ion exchange. chromatography, affinity chromatography, size exclusion chromatography, HPLC, or a combination thereof.

As used herein, the term "extract" refers to an extract obtained by extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, the extract itself and the extract. It includes extracts of all formulations that can be formed using

In the present invention, "antibacterial functionality" is to block and inhibit the propagation of microorganisms such as bacteria, bacteria and fungi, and includes the effect of preventing the propagation of bacteria, bacteria and fungi caused by the external environment or preventing viral infection. The composition according to the present application may be used for various purposes requiring inhibition of bacteria in addition to the above-described antibacterial function.

The mixed extract containing the gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract of the present invention as active ingredients has an amount of 0.001 to 40, 0.001 to 39, 0.001, based on the total weight of the cosmetic composition. to 38, 0.001 to 37, or 0.001 to 36% by weight, and preferably 0.001 to 35% by weight in terms of achieving excellent antibacterial activity and stable formulation by including the mixed extract in an appropriate amount in the composition, but , but is not limited thereto. In the present invention, when the content of the mixed extract is less than 0.001% by weight, the effect of inhibiting bacterial growth is insignificant, and when it exceeds 40% by weight, the intended formulation is difficult and the feeling of use is reduced.

The gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract of the present invention may be mixed in a weight ratio of 1:0.5 to 5:2 to 7, preferably 1:1 to It may be mixed in a weight ratio of 5: 3 to 7, more preferably a cosmetic composition mixed in a weight ratio of 1: 3: 3, 1: 4: 4, 1: 1: 7, but is not limited thereto. .

The cosmetic composition mixes and includes the extracts at a constant ratio within the above range, compared to the case of containing only one of the Gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract. It can have anti-proliferation and anti-inflammatory and antioxidant effects.

The mixed extract of the present invention is a cosmetic composition further comprising any one or more of 3-0-Ethyl Ascorbic acid and Panthenol in terms of exhibiting effective anti-inflammatory and antioxidant effects. can be

The 3-0-ethyl ascorbic acid (3-0-Ethyl Ascorbic acid) is a water-soluble vitamin. It is present in citrus fruits, tomatoes, strawberries and many other fruits and vegetables, can be obtained synthetically, and its deficiency causes scurvy.

Panthenol is a water-soluble vitamin that is easily absorbed into the body as one of the essential nutrients and is converted into a component called pantothenic acid through a chemical reaction. Therefore, it can be widely used as a stable component.

The mixed extract including the gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol as active ingredients of the present invention is a cosmetic composition in total 0.001 to 40, 0.001 to 39, 0.001 to 38, 0.001 to 37, or 0.001 to 36% by weight, and the mixed extract is contained in an appropriate amount in the composition to effectively inhibit the growth of flora on the skin, resulting in excellent In terms of achieving antibacterial activity and formulating a stable viscosity, the composition may be preferably included in an amount of 0.001 to 35% by weight, but is not limited thereto. In the present invention, when the content of the mixed extract is less than 0.001% by weight, the effect of inhibiting bacterial growth is insignificant, and when it exceeds 40% by weight, the acidic component 3-O-ethyl ascorbic acid causes a decrease in viscosity when applied to creams or fixed formulations. collapses, making it difficult to formulate the desired product and reducing the feeling of use.

The ratio of the Gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol of the present invention is 1 : 0.5-5 : 2-7 : 0.05 ~ 0.3: may be mixed in a weight ratio of 0.1 ~ 0.8, preferably 1: 1 ~ 5: 3 ~ 7: 0.1 ~ 0.3: may be mixed in a weight ratio of 0.3 ~ 0.7, more preferably 1: 3: 3: 0.1: 0.5, 1: 3: 3: 0.2: 0.5, 1: 4: 4: 0.1: 0.5, 1: 4: 4: 0.2: 0.5, 1: 1: 7: 0.1: 0.5, 1: 1: It may be a cosmetic composition that can be mixed in a weight ratio of 7: 0.2: 0.5, but is not limited thereto.

The gardenia flower extract may be 3.89 to 11.59, 4 to 11.5, 4.5 to 11.4, 5 to 11.3, 6 to 11.2 or 7 to 11.2% by weight based on the total weight of the cosmetic composition, and in terms of maintaining excellent anti-inflammatory and antioxidant effects, Depending on the embodiment, it may preferably be 8.3 to 11.11% by weight.

The Pelargonium graveolens extract may be 3.89 to 11.59, 4 to 11.5, 4.5 to 11.4, 5 to 11.3, 6 to 11.2 or 7 to 11.2% by weight relative to the total weight of the cosmetic composition, maintaining excellent anti-inflammatory and antioxidant effects In terms of that, it may be preferably 8.3 to 11.11% by weight according to one embodiment.

The Salvia officinalis extract may be 3.89 to 11.59, 4 to 11.5, 4.5 to 11.4, 5 to 11.3, 6 to 11.2 or 7 to 11.2% by weight based on the total weight of the cosmetic composition, and maintain excellent anti-inflammatory and antioxidant effects. In terms of that, it may be preferably 8.3 to 11.11% by weight according to one embodiment.

0.12 to 13.46, 0.2 to 12, 0.3 to 11, 0.4 to 10, 0.5 to 9, 0.6 to 8, 0.7 to 7, 0.8 to 6, 0.7 to 5, based on the total weight of the cosmetic composition. , 0.8 to 4, 0.9 to 3, and may be 1.0 to 2% by weight, and may be preferably 1.11 to 1.89% by weight according to one embodiment in terms of maintaining excellent anti-inflammatory and antioxidant effects.

The panthenol may be 0.23 to 15.55, 1 to 15, 2 to 14, 3 to 13, 4 to 10.5, and 5 to 9% by weight based on the total weight of the cosmetic composition, and in one embodiment in terms of maintaining excellent anti-inflammatory and antioxidant effects Depending on this, it may be preferably 5.55 to 8.54% by weight.

The cosmetic composition contains the extracts more than any one of the Gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethylascorbic acid, and the panthenol. By including and mixing in a certain ratio within the above range, it can have excellent antibacterial activity and anti-inflammatory and antioxidant effects.

The present inventors have prepared the above-mentioned gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethylascorbic acid and the panthenol as active ingredients in a specific mixing ratio or weight ratio. In the case of the cosmetic composition containing, it was confirmed that it can be used as an antibacterial cosmetic composition by effectively inhibiting the proliferation of skin flora and exhibiting excellent antibacterial activity. In addition, it was specifically confirmed that the composition exhibited excellent anti-inflammatory and anti-aging activities, and even a skin improvement effect.

In this specification, the term "cosmetic composition" means an article used on the human body to clean and beautify the human body to add charm, brighten the appearance, or maintain or promote the health of skin and hair. The cosmetic composition may be for antibacterial purposes.

Here, the skin flora is, for example, Escherichia coli ( E. coli ), Candida albicans ( Candida albicans ), Bacillus subtilis ( Bacillus subtilis ), Staphylococcus aureus ( Staphylococcus aureus, S. aureus ), Aspergillus niger ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), Escherichia coli ( Escherichia coli ), Micrococcus luteus ( Micrococcus lutues ), Klebsiella new moniee ( Klebsiella pnemoniae ) , and Staphylococcus epidermidis ( Staphylococcus epidermidis ) It may be any one or more selected from the group consisting of.

The cosmetic composition of the present invention may be a cosmetic composition containing at least one selected from the group consisting of thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, and fragrances. In the cosmetic composition, other ingredients and functional ingredients that are formulated in conventional cosmetics may be additionally formulated.

For example, in terms of formulating a stable viscosity, preferably 1.0 to 2.0% by weight of a preservative, 0.01 to 0.5% by weight of a thickener, 0.03% by weight of a stabilizer, the balance of which is purified water, based on the total weight of the cosmetic composition. It may be one or more selected from. Here, the stabilizer may be, for example, a chelating agent.

Other ingredients that may be added include fats and oils, humectants, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, A blood circulation accelerator, a cooling agent, an antiperspirant, purified water, etc. are mentioned.

The cosmetic composition of the present invention may be a cosmetic composition having anti-inflammatory, antioxidant, and anti-aging functions.

The cosmetic composition may be intended to block and inhibit the propagation of microorganisms such as bacteria, bacteria, mites, and fungi, and viruses. However, it is not limited thereto.

The skin includes all skin areas of the body, including the face, hands, arms, legs, feet, chest, stomach, back, buttocks, and scalp.

As used herein, the terms "applying", "administering", and "applying" are used interchangeably and result in at least partial localization of a composition according to an embodiment to a desired site, or route of administration. It may mean disposing of a composition according to an embodiment into an object by.

According to another embodiment of the present invention, gardenia flower ( Gardenia Florida Flower ), Pelargonium Graveolens ( Pelargonium Graveolens ) and Salvia Officinalis ( Salvia Officinalis (Sage)) raw materials are ultrasonically extracted to obtain extracts It relates to a method for producing an antibacterial functional cosmetic composition comprising the step.

In the extraction of the present invention, the extraction method is not particularly limited, and extraction may be performed according to hot water extraction, ultrasonic extraction, filtration, reflux extraction, etc., which are commonly used in the art, but preferably ultrasonic extraction can

The extraction may be repeated one or more times, for example, 1 to 5 times, 2 to 4 times, or 3 times, and each extraction may be performed in the same way or in a different way. Specifically, for example, each gardenia flower extract, Pelargonium graveolens extract, and Salvia officinalis extract were added with 1 to 15 times the weight of the extraction solvent compared to each raw material, and then at 15 to 25 ° C for 30 minutes to 3 hours. It may be obtained by low-temperature ultrasonic treatment at a frequency of 20 to 40 kHz, and more specifically, the low-temperature ultrasonic treatment may be performed at a frequency of 22 to 38 kHz for 30 minutes to 2 hours at a temperature of 17 to 22 ° C.

In the present invention, the type of extraction solvent used to extract Gardenia, Pelargonium graveolens, and Salvia officinalis is not particularly limited, and any solvent known in the art may be used. In the present invention, the extract may be obtained by extraction with water as a hydrophilic solvent, C ₁ to C ₁₀ monohydric and dihydric alcohols, and trihydric alcohols or mixed solvents thereof. In addition, non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; and polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; Or a mixture thereof may be used, and specifically, methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, isobutanol, tert-butanol, and butylene glycol may be used alone or in combination of two or more. and can be used.

The extraction may further include a process of separating the plant residue and the extract by a known method such as filtration, and may include a step of removing the solvent from the obtained extract by a known method such as concentration under reduced pressure. It may also include preparing a dried extract by drying the obtained extract such as nitrogen drying or freeze drying. The extract may be in the form of a concentrated solution, or after being dried and then re-solvated with an appropriate solvent such as DMSO, or freeze-dried and powdered.

The extraction may further include subjecting the crude extract to silica gel column chromatography or Sephadex LH-20 chromatography.

In the present invention, drying of the extract may be performed by a known method within the range of not destroying useful components from the collected plant, and may be performed, for example, by natural drying in the shade. In addition, crushing or pulverization may be pulverized by crushing or pulverizing to the extent that useful components of the plant can be sufficiently extracted in the subsequent extraction process. The drying and crushing or crushing process may be carried out in reverse order or repeatedly if necessary.

The present invention is provided based on the results of the following experiments. The antibacterial functional cosmetic composition of the present invention contains a mixed extract of the gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract as active ingredients. It is characterized in that it comprises, and if the form including the antibacterial functional cosmetic composition, it can be usefully used in the group related to cosmetics, health functional foods, skin external preparations, quasi-drugs and pharmaceutical compositions.

Also, in the present invention, "active ingredient" means a component that exhibits the desired activity alone or that can exhibit activity in combination with a carrier having no activity itself.

The Gardenia Florida Flower extract, the Pelargonium Graveolens extract, and the Salvia Officinalis (Sage) extract of the present invention exhibit effective anti-inflammatory and antioxidant effects On the other hand, it may be a method for preparing an antimicrobial functional cosmetic composition comprising mixing at least one of 3-0-ethyl ascorbic acid and panthenol. For example, the Gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethylascorbic acid and the panthenol are 1: 0.5-5: 2-7: 0.05 to 0.3: may be mixed in a weight ratio of 0.1 to 0.8, preferably 1: 1 to 5: 3 to 7: 0.1 to 0.3: may be mixed in a weight ratio of 0.3 to 0.7.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited by the following examples.

Example

Preparation Example 1: Preparation of gardenia flower extract

Take 25 g of raw material (UK) for Gardenia Florida Flower , dry it, cut it into 1 to 5 cm pieces, put it in a container, and use 150 mL of 30 wt% butylene glycol as an extraction solvent at a temperature of 85 ° C. A 5% Gardenia flower extract was prepared by hot water extraction for 2 hours or ultrasonic extraction at a temperature of 20° C. for 1 hour at a frequency of 30 kHz. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Preparation Example 2: Preparation of Pelargonium graveolens extract

Take 25g of raw material (UK) for Pelargonium Graveolens , dry it, cut it into pieces of 1 to 5cm, put it in a container, and 85 ℃ with 150mL of 30% by weight of butylene glycol as an extraction solvent A 5% Pelargonium graveolens extract was prepared by hot water extraction at a temperature of 2 hours for 2 hours or ultrasonic extraction at a temperature of 20° C. for 1 hour at a frequency of 30 kHz. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Preparation Example 3: Preparation of Salvia Officinalis Extract

Take 25 g of raw material (Germany) for Salvia Officinalis (Sage), dry it, cut it into pieces of 1 to 5 cm, put it in a container, and use 150 mL of 30% by weight of butylene glycol as an extraction solvent 5% Salvia officinalis extract was prepared by hot water extraction at a temperature of 85° C. for 2 hours or ultrasonic extraction at a temperature of 20° C. for 1 hour at a frequency of 30 kHz. Then, the final extract was dissolved in water at a concentration of 1% by weight and used in the following experimental examples.

Experimental Example 1: Efficacy evaluation according to manufacturing method

1-1. Analysis of antibacterial activity of hot water extract and ultrasonic extract

Each of the hot water extract and the ultrasonic extract prepared in Preparation Examples 1 to 3 is used for Escherichia coli ( Escherichia coli ) and skin flora, Staphylococcus aureus ( Staphylococcus aureus , S. aureus ), which is an antibacterial disk diffusion method. (Disc diffusion assay, clear zone).

Each mixture prepared by hot water and ultrasonic extraction according to Preparation Examples 1 to 3 was set as a sample, and 0.025% of tetracycline, an antibacterial substance, was set as a positive control. First, after activating two stock strains ( E.coli, S.aureus ) in a liquid medium, the primary culture was performed. Each strain was smeared with the primary culture medium on a suitable solid medium (using a sterilized cotton swab), dried, and then sterilized Paper disc 8mm Thin was attached so that the flat surface was in contact with the solid medium. Next, the samples of the positive control and Preparation Examples 1 to 3 were dispensed on each 20ul Paper disc, cultured at 37 ° C for 24H, and the final clear zone diameter was measured to confirm antibacterial activity. The results of the above antibacterial experiments are shown in Table 1 below.

Extraction method	strain type	positive control	gardenia	pelargonium graveolens	Salvia officinalis
hydrothermal	E. coli	17	X	X	X
	S. aureus	17	X	X	10
ultrasonic wave	E. coli	17	X	X	X
	S. aureus	17	9	9	10

As shown in Table 1, in the case of Preparation Examples 1 to 3 prepared by hot water extraction, only the Salvia officinalis extract of Preparation Example 3 showed antibacterial activity in S. aureus , and Preparation Examples 1 to 3 prepared by ultrasonic extraction In case 3, it was confirmed that antibacterial activity appeared only in S. aureus .

1-2. Confirmation of anti-inflammatory and antioxidant effects of hot water extract and ultrasonic extract

When extracts of Gardenia flower, Pelargonium graveolens, and Salvia officinalis were prepared by the methods of Preparation Examples 1 to 3, whether the skin improvement effect, specifically, the anti-inflammatory and antioxidant effects could be changed by the extraction method was investigated. An experiment was conducted to confirm.

Specifically, in order to confirm the anti-inflammatory effect, an experiment was performed to determine whether the production of nitric oxide could be inhibited, and an experiment was performed to confirm the DPPH free radical scavenging activity to confirm the antioxidant activity. The results of the compositions prepared by hot water or ultrasonic extraction of Preparation Examples 1 to 3 are shown in Table 2 below.

Extraction method	Efficacy (%)	gardenia	pelargonium graveolens	Salvia officinalis
hot water extract	Anti-inflammatory (NO)	48.5	55.1	70.6
	Antioxidant (DPPH)	45.8	60.6	55.2
ultrasonic extract	Anti-inflammatory (NO)	55.6	60.1	86.3
	Antioxidant (DPPH)	69.5	88.5	79.5

As shown in Table 2, in the anti-inflammatory and antioxidant experiments, it was confirmed that each of the compositions extracted through ultrasonic extraction had higher anti-inflammatory and antioxidant effects than the hot water extract, and thus had excellent skin improvement effects. Therefore, in the following examples, the skin improvement effect was confirmed using the ultrasonic extract.

Experimental Example 2: Efficacy evaluation according to mixing ratio

Compositions prepared by the ultrasonic extraction method of Preparation Examples 1 to 3 were mixed in the mixing ratio of Examples 1 to 3 and Comparative Examples 1 to 4 shown in Table 3, 30% by weight of the mixed extract, 1% by weight of preservative, 0.2% by weight of thickener. %, stabilizer 0.03% by weight and the balance of purified water was mixed to prepare a cosmetic composition.

division	gardenia extract	Pelargonium Graveolens Extract	Salvia Officinalis Extract	3-0-Ethyl Ascorbic Acid	panthenol
Example 1	One	3	3	0.2	0.5
Example 2	One	4	4	0.2	0.5
Example 3	One	One	7	0.2	0.5
Comparative Example 1	One	One	One	0.1	0.5
Comparative Example 2	One	One	One	0.4	1.0
Comparative Example 3	One	7	One	0.2	0.5
Comparative Example 4	One	4	8	0.2	0.5

2-1. Analysis of antibacterial activity by ultrasonic extraction

Each of the mixtures according to Table 3 above has an antibacterial activity against Escherichia coli (E. coli ) and the skin flora, Staphylococcus aureus (S.aureus ). Disc diffusion assay, clear zone ) was confirmed through.

Each of the mixtures according to Table 3 was set as a sample, and 0.025% of tetracycline, an antibacterial substance, was set as a positive control group, and 30% BG was used as a negative control group. As a control, a commercially available hand sanitizer (ethanol 62%, Korea Life Science Co., Ltd.) consisting of ethanol, glycerin, aloe extract, and purified water was used.

First, after activating two stock strains ( E.coli, S.aureus ) in a liquid medium, the primary culture was performed. Each strain was smeared with the primary culture medium on a suitable solid medium (using a sterilized cotton swab), dried, and then sterilized Paper disc 8mm Thin was attached so that the flat surface was in contact with the solid medium. Next, the positive control, negative control, commercially available hand sanitizer and sample were dispensed on each 20ul Paper disc, and after incubation at 37 ° C. for 24H, the final clear zone diameter was measured to confirm antibacterial activity. The results of the above antibacterial experiments are shown in Tables 4 and 5 below.

strain type	positive control	negative control group	Example 1	Example 2	Example 3	Hand sanitizer
E. coli	13	X	8	11	8	X
S. aureus	26	X	11	20	10	X

As shown in Table 4, it was confirmed that all of Examples 1 to 3 exhibited antibacterial activity in E.coli and the skin flora, Staphylococcus auris ( S.aureus ).

Specifically, it was confirmed that the mixture of Example 2 exhibited antibacterial activity similar to that of the positive control group. However, in the case of commercially available hand sanitizers, it was confirmed that they did not show any antibacterial activity against the above two strains.

strain type	Comparative Example 1	Comparative Example 2	Comparative Example 3	Comparative Example 4
E. coli	X	X	X	X
S. aureus	10	10	9	10

Referring to Table 4 to Table 5, according to the embodiments of the present invention, gardenia flower extract, Pelargonium graveolens extract, Salvia officinalis extract, 3-0-ethyl ascorbic acid and panthenol were added to 1 : 0.5 ~ 5 : 2 ~ 7 : 0.05 ~ 0.3 : 0.1 ~ 0.8 When the mixed mixing ratio is satisfied, it can be confirmed that the antibacterial activity is excellent, and it can be confirmed that it is suitable for preparing a liquid formulation with a desired viscosity. can In addition, Examples 1 to 3 and Comparative Examples 3 and 4 in which the ratios of 3-0-ethyl ascorbic acid and panthenol were set at fixed values and Comparative Example 1 containing different ratios of 3-0-ethyl ascorbic acid and panthenol and 2, it can be seen that the proportions of Gardenia flower extract, Pelargonium graveolens extract and Salvia officinalis extract are more important in antibacterial activity, and compared to Gardenia flower extract, Pelargonium graveolens extract and In the case of Example 2, in which the content of the Salvia officinalis extract was high, it was confirmed that antibacterial activity similar to that of the positive control group appeared. Specifically, both Comparative Examples 1 and 2 do not exhibit antibacterial activity in E.coli , and it can be seen that the antibacterial activity in S. aureus is also significantly lower than in Example 2.

In the case of Comparative Example 3, when the Pelargonium graveolens extract is out of the mixing ratio of the present invention, it can be confirmed that the antibacterial activity is low compared to the Example. Specifically, it can be seen that E. coli does not exhibit antibacterial activity, and the antibacterial activity of S. aureus is also remarkably low compared to Example 2. Therefore, it can be seen that when the Pelargonium graveolens extract is in the mixed mixing ratio of the present invention, excellent antibacterial activity is exhibited against all of the skin flora.

In the case of Comparative Example 4, when the Salvia officinalis extract is out of the mixing ratio of the present invention, it can be seen that the antibacterial activity is generally low compared to Examples 1 to 3. Specifically, when Comparative Example 4 was compared to Example 2, in which only the compounding ratio of the Salvia officinalis extract was different, it was confirmed that E. coli did not exhibit antibacterial activity and that S. aureus also had significantly lower antibacterial activity. there is. Therefore, it can be seen that when the Salvia officinalis extract is in the mixed mixing ratio of the present invention, excellent antibacterial activity is exhibited against all of the skin flora.

In this way, when the skin flora is not properly controlled, there are problems of causing itching, skin dryness, and even an inflammatory reaction.

In particular, in the case of the comparative examples, only the antibacterial activity against the specific bacterium S.aureus was shown, and in the case of the examples, the antibacterial activity against not only E.coli but also against the S.aureus strain was confirmed. It can be seen that the antibacterial efficacy of the composition according to the embodiments of the present invention is greater because various common bacteria exist on the skin and the antibacterial efficacy against each microorganism can act in combination.

2-2. Analysis of anti-inflammatory and antioxidant effects according to ultrasonic extraction

An experiment was performed to confirm the skin improvement effect, specifically the anti-inflammatory and antioxidant effect of each of the mixtures according to Table 3 above.

In order to confirm the anti-inflammatory effect, an experiment was performed to determine whether the production of nitric oxide could be inhibited, and to confirm the antioxidant activity, an experiment was performed to confirm the DPPH free radical scavenging activity. Ethanol was used as a control. , A commercially available hand sanitizer (ethanol 62%, Korea Life Science Co., Ltd.) consisting of glycerin, aloe extract, and purified water was used. The results of the above anti-inflammatory and antioxidant experiments are shown in Tables 6 and 7 below.

Efficacy (%)	Example 1	Example 2	Example 3	Hand sanitizer
Anti-inflammatory (NO)	65.2	95.6	96.8	X
Antioxidant (DPPH)	90.5	92.5	81.6	X

As shown in Table 6, in the case of the hand sanitizer used as a control example, it was confirmed that antioxidant and anti-inflammatory activities did not appear, but the mixtures of Examples 1 to 3 were generally excellent in anti-inflammatory and antioxidant activities, resulting in a skin improvement effect. could be seen appearing.

Efficacy (%)	Comparative Example 1	Comparative Example 2	Comparative Example 3	Comparative Example 4
Anti-inflammatory (NO)	60.5	68.1	52.5	50.1
Antioxidant (DPPH)	70.7	80.3	95.2	62.1

Referring to Tables 6 to 7, according to the embodiments of the present invention, gardenia flower extract, Pelargonium graveolens extract, Salvia officinalis extract, 3-0-ethyl ascorbic acid and panthenol were mixed with 1 : 0.5 ~ 5 : 2 ~ 7 : 0.05 ~ 0.3 : 0.1 ~ 0.8, when the mixed mixing ratio is satisfied, it can be confirmed that the anti-inflammatory and antioxidant activity is excellent and has a skin improvement function, and the desired viscosity It can be confirmed that it is suitable for the preparation of a liquid formulation.

In addition, Comparative Example 1 containing Examples 1 to 3 and Comparative Examples 3 and 4 with fixed ratios of 3-0-ethyl ascorbic acid and panthenol at different ratios of 3-0-ethyl ascorbic acid and panthenol and 2, it can be confirmed that the proportions of gardenia flower extract, Pelargonium graveolens extract and Salvia officinalis extract are important in anti-inflammatory and antioxidant functions, and Pelargonium graveolens extract and Salvia In the case of Example 2, in which the ratio of the Officinalis extract content was all high, it could be confirmed that the anti-inflammatory and antioxidant effects were the best.

In the case of Comparative Example 3, when the Pelargonium graveolens extract was out of the mixing ratio of the present invention, it could be confirmed that the anti-inflammatory efficacy was remarkably low compared to Examples 1 to 3. In addition, since the antioxidant efficacy of Comparative Example 3 was somewhat high, it was found that the higher the content ratio of Pelargonium graveolens, the better the antioxidant effect, but there is a problem in that both anti-inflammatory and antioxidant activities are not excellent. Therefore, it can be seen that when the Pelargonium graveolens extract is in the mixed mixing ratio of the present invention, both anti-inflammatory and antioxidant activities are excellent, and the skin improvement effect is remarkable.

In the case of Comparative Example 4, when the Salvia officinalis extract was out of the mixing ratio of the present invention, it could be confirmed that the anti-inflammatory and antioxidant efficacy was significantly lower than that of Examples 1 to 3. Specifically, when compared to Example 2, in which only the compounding ratio of Comparative Example 4 and the Salvia officinalis extract was different, it can be seen that the higher the content of the Salvia officinalis extract, the better the anti-inflammatory and antioxidant effect. It can be seen that the skin improvement effect is remarkable when the ratio of the Via officinalis extract is the mixed mixing ratio of the present invention.

Therefore, as can be seen in Tables 4 to 7, Gardenia flower extract, Pelargonium graveolens extract, Salvia officinalis extract, 3-0-ethylascorbic acid and panthenol were prepared in Example 1 according to the present invention. to 3, compared to Comparative Examples 1 to 4, not only improved antibacterial activity against all of the skin flora, but also excellent anti-inflammatory and antioxidant effects, useful as an antibacterial composition with remarkable skin improvement effect It was confirmed that there is a critical significance that can be utilized appropriately.

Having described specific parts of the present invention in detail above, it is clear that these specific techniques are only preferred embodiments for those skilled in the art, and the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (9)

1. An antimicrobial functional cosmetic composition comprising a mixed extract of Gardenia Florida Flower extract, Pelargonium Graveolens extract and Salvia Officinalis (Sage) extract, and purified water.
2. According to claim 1,

   The cosmetic composition of claim 1, wherein the gardenia flower extract, the Pelargonium graveolens extract, and the Salvia officinalis extract are mixed in a weight ratio of 1: 0.5 to 5: 2 to 7.
3. According to claim 1,

   The mixed extract is a cosmetic composition further comprising any one or more of 3-0-ethyl ascorbic acid and panthenol.
4. According to claim 3,

   The mixed extract is a cosmetic composition comprising 0.001 to 40% by weight based on the total weight of the cosmetic composition.
5. According to claim 3,

   The Gardenia flower extract, the Pelargonium graveolens extract, the Salvia officinalis extract, the 3-0-ethyl ascorbic acid and the panthenol are 1: 0.5-5: 2-7: 0.05-0.3: A cosmetic composition mixed in a weight ratio of 0.1 to 0.8.
6. According to claim 1,

   The cosmetic composition is a cosmetic composition comprising at least one selected from the group consisting of thickeners, preservatives, stabilizers, solubilizers, surfactants, carriers, and fragrances.
7. According to claim 1,

   The cosmetic composition is a cosmetic composition having anti-inflammatory, antioxidant, anti-aging functions.
8. Gardenia flower ( Gardenia Florida Flower ), Pelargonium Graveolens ( Pelargonium Graveolens ) and Salvia Officinalis ( Salvia Officinalis (Sage)) of an antibacterial functional cosmetic composition comprising the step of obtaining an extract by ultrasonic extraction manufacturing method.
9. According to claim 8,

   Gardenia Florida Flower Extract, Pelargonium Graveolens Extract and Salvia Officinalis (Sage) Extract with 3-0-Ethyl Ascorbic Acid Ascorbic acid) and panthenol (Panthenol) a method for producing an antibacterial functional cosmetic composition comprising the step of mixing any one or more.