WO2023104218A3 - Pcr热循环装置及控制方法 - Google Patents

Pcr热循环装置及控制方法 Download PDF

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Publication number
WO2023104218A3
WO2023104218A3 PCT/CN2023/071084 CN2023071084W WO2023104218A3 WO 2023104218 A3 WO2023104218 A3 WO 2023104218A3 CN 2023071084 W CN2023071084 W CN 2023071084W WO 2023104218 A3 WO2023104218 A3 WO 2023104218A3
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WO
WIPO (PCT)
Prior art keywords
temperature
thermostatic bath
capillary tube
sample
thermal cycling
Prior art date
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PCT/CN2023/071084
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English (en)
French (fr)
Other versions
WO2023104218A2 (zh
Inventor
李晓峰
何马强
张涛
Original Assignee
广东润鹏生物技术有限公司
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Application filed by 广东润鹏生物技术有限公司 filed Critical 广东润鹏生物技术有限公司
Publication of WO2023104218A2 publication Critical patent/WO2023104218A2/zh
Publication of WO2023104218A3 publication Critical patent/WO2023104218A3/zh

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

本申请提供一种PCR热循环装置及控制方法，属于快速分子诊断技术领域。该PCR热循环装置包括具有恒定的第一温度的第一恒温槽、移动机构和控制模块，该移动机构被配置成将毛细管和测温模块同步移动至第一恒温槽内或从第一恒温槽内取出，以通过第一恒温槽对装于毛细管中的样品进行温度控制；该测温模块被配置成检测温度，该控制模块被配置成：控制模块设置有第一设定温度，当该测温模块在第一恒温槽内测得的温度到达第一设定温度时，该控制模块立即控制该移动机构将毛细管从第一恒温槽内取出，从而通过测温模块间接地对样品的温度进行监测，保证毛细管内样品的温度到达第一温度阈值时能够被及时从第一恒温槽内取出。
PCT/CN2023/071084 2021-12-06 2023-01-06 Pcr热循环装置及控制方法 WO2023104218A2 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202111480728.5 2021-12-06
CN202111480728.5A CN114134033B (zh) 2021-12-06 2021-12-06 Pcr热循环装置及控制方法

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WO2023104218A2 WO2023104218A2 (zh) 2023-06-15
WO2023104218A3 true WO2023104218A3 (zh) 2023-08-17

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114134033B (zh) * 2021-12-06 2022-11-04 广东润鹏生物技术有限公司 Pcr热循环装置及控制方法

Citations (5)

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CN104263634A (zh) * 2014-09-24 2015-01-07 中国科学技术大学 一种基于毛细管的流式聚合酶链式反应循环加热仪及加热方法
CN106978328A (zh) * 2016-01-15 2017-07-25 北京酷搏科技有限公司 热循环反应组件及具有其的实时检测装置
CN109562383A (zh) * 2016-06-10 2019-04-02 星阵私人有限公司 用于样品分析与处理的快速热循环
CN109797091A (zh) * 2019-01-17 2019-05-24 浙江大学 一种旋转式快速双温pcr扩增自动控制装置和控制方法
CN114134033A (zh) * 2021-12-06 2022-03-04 广东润鹏生物技术有限公司 Pcr热循环装置及控制方法

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US6787338B2 (en) * 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
CN106222068A (zh) * 2016-08-22 2016-12-14 上海交通大学 玻璃毛细管微型pcr系统及其制备方法
CN106442454A (zh) * 2016-11-17 2017-02-22 中国科学院上海光学精密机械研究所 荧光定量基因快速扩增检测装置和扩增检测方法
CN206385166U (zh) * 2017-01-19 2017-08-08 北京凡知医学科技有限公司 一种dna扩增装置
CN110387325A (zh) * 2019-08-13 2019-10-29 广州市华南医学研究中心 一种极速pcr反应检测装置及检测方法
CN111023613A (zh) * 2019-11-27 2020-04-17 合肥通用制冷设备有限公司 一种精确控温制冷系统

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104263634A (zh) * 2014-09-24 2015-01-07 中国科学技术大学 一种基于毛细管的流式聚合酶链式反应循环加热仪及加热方法
CN106978328A (zh) * 2016-01-15 2017-07-25 北京酷搏科技有限公司 热循环反应组件及具有其的实时检测装置
CN109562383A (zh) * 2016-06-10 2019-04-02 星阵私人有限公司 用于样品分析与处理的快速热循环
CN109797091A (zh) * 2019-01-17 2019-05-24 浙江大学 一种旋转式快速双温pcr扩增自动控制装置和控制方法
CN114134033A (zh) * 2021-12-06 2022-03-04 广东润鹏生物技术有限公司 Pcr热循环装置及控制方法

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CN116218660A (zh) 2023-06-06
CN114134033B (zh) 2022-11-04
WO2023104218A2 (zh) 2023-06-15
CN114134033A (zh) 2022-03-04
CN116286320A (zh) 2023-06-23

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