WO2023050710A1 - 一种多通道lamp检测仪及其控制方法 - Google Patents

一种多通道lamp检测仪及其控制方法 Download PDF

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Publication number
WO2023050710A1
WO2023050710A1 PCT/CN2022/078304 CN2022078304W WO2023050710A1 WO 2023050710 A1 WO2023050710 A1 WO 2023050710A1 CN 2022078304 W CN2022078304 W CN 2022078304W WO 2023050710 A1 WO2023050710 A1 WO 2023050710A1
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WIPO (PCT)
Prior art keywords
detection
chip
detection signal
channel
controller
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PCT/CN2022/078304
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English (en)
French (fr)
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颜菁
俞涛
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江苏汇先医药技术有限公司
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Publication of WO2023050710A1 publication Critical patent/WO2023050710A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the invention belongs to the technical field of nucleic acid detection, and relates to a multi-channel LAMP detector and a control method thereof.
  • LAMP Loop-mediated isothermal amplification
  • Loop-mediated isothermal amplification technology is widely used in the field of biological diagnosis due to its mild reaction conditions (lower reaction temperature) and short reaction time, such as nucleic acid amplification detection to diagnose samples presence of pathogens.
  • LAMP technology is to provide in vitro amplification conditions for nucleic acid fragments to make them exponentially amplify in large quantities and add fluorescent dyes or fluorescent markers during the nucleic acid amplification process, and use optical devices to detect the strength of fluorescent signals. The process by which the analysis of fluorescent signals yields the results of nucleic acid amplification. During the nucleic acid amplification reaction, the reaction system needs to be heated.
  • the current LAMP detector can integrate nucleic acid amplification detection.
  • the detection chip usually a microfluidic chip
  • the reaction chamber usually an amplification reaction chamber
  • the sample volume to be detected is large, a LAMP detector capable of detecting multiple samples at one time is required.
  • an object of the present invention is to provide a multi-channel LAMP detector, which can automatically detect samples in multiple microfluidic chips at one time, improve detection efficiency, and has a small volume.
  • Another object of the present invention is to provide a control method for a multi-channel LAMP detector, which automatically detects samples of multiple microfluidic chips at one time without additional device cost, thereby improving detection efficiency.
  • a multi-channel LAMP detector includes a chip chamber and an optical detection mechanism, the chip chamber has a plurality of chip slots for accommodating microfluidic chips, and a plurality of the chips The slots are arranged side by side along the front-to-back direction; the optical detection mechanism is arranged in the housing so as to be movable along the left-right direction and the front-to-back direction, and the optical detection mechanism is located below the chip compartment; each of the chip slots has A plurality of detection positions arranged along the left and right directions, the optical detection mechanism has a plurality of detection positions, and each detection position of each chip slot corresponds to one detection position; the multi-channel LAMP The detector also includes:
  • X-direction driving mechanism which is used to drive the optical detection mechanism to move along the left and right directions;
  • Y-direction driving mechanism which is used to drive the optical detection mechanism to move along the front and rear directions
  • X-direction starting point detection switch which is used to send a first detection signal when detecting that the optical detection mechanism reaches its starting point in the left-right direction;
  • X-direction end point detection switch which is used to send a second detection signal when detecting that the optical detection mechanism reaches its end point in the left and right direction;
  • Y-direction starting point detection switch which is used to detect the third detection signal that the optical detection mechanism reaches its starting point in the front-back direction
  • Y direction end detection switch which is used to detect the fourth detection signal that the optical detection mechanism reaches its end point in the front-back direction
  • a controller which is used to receive the detection signals of the X-direction start detection switch, X-direction end detection switch, Y-direction start detection switch, and Y-direction end detection switch, and can control all
  • the X-direction drive mechanism runs forward, and can control the X-direction drive mechanism to run backward after receiving the second detection signal, and can control the Y-direction drive after receiving the third detection signal
  • the mechanism runs forward, and can control the Y-direction driving mechanism to run reversely after receiving the fourth detection signal.
  • the controller is configured to, when receiving the first detection signal and the third detection signal, control the X-direction driving mechanism to run forward for a first set distance Stop setting the time, repeat this process one or more times, until the second detection signal is received, control the X-direction drive mechanism to run in reverse; until the first detection signal is received again, control the The X-direction drive mechanism stops running.
  • the controller is further configured to control the Y-direction driving mechanism to run forward for a second set distance and then stop when receiving the first detection signal and the third detection signal Set the time; control the X-direction drive mechanism to run forward for the first set distance and then stop the set time, repeat this process one or more times until the second detection signal is received, control the X-direction The driving mechanism runs in reverse; until the first detection signal is received again, the X-direction driving mechanism is controlled to stop running.
  • the controller is further configured to control the Y-direction driving mechanism to run forward when receiving the first detection signal, until the fourth detection signal is received, to control the The Y-direction drive mechanism stops running; the X-direction drive mechanism is controlled to run forward for a first set distance and then stops for a set time, repeating this process one or more times until the second detection signal is received, and the control The X-direction drive mechanism runs in reverse; until the first detection signal is received again, the X-direction drive mechanism is controlled to stop running.
  • the controller is further configured to control the Y-direction driving mechanism to run in reverse when receiving the fourth detection signal and the first detection signal until the first detection signal is received.
  • Three detection signals to control the Y-direction drive mechanism to stop running.
  • the set times are equal.
  • the X-direction drive mechanism includes an X-direction motor
  • the Y-direction drive mechanism includes a Y-direction motor
  • the controller is electrically connected to the X-direction motor and the Y-direction motor respectively. connect.
  • the controller is electrically connected to the X-direction start detection switch, the X-direction end detection switch, the Y-direction start detection switch and the Y-direction end detection switch respectively
  • the multi-channel LAMP detector also includes a scanner for reading the barcode on the microfluidic chip, and a display screen used as a human-computer interaction interface, the display screen has a first A display state, in the first display state, the display screen has a code-scanning entry button for the user to input a code-scanning entry instruction, and the controller is also used to Establish a correspondence relationship between the barcode information read by the scanner and the corresponding detection channels according to the set order, and each detection channel corresponds to one of the chip slots.
  • the controller is also used to receive the detection data returned by the optical detection mechanism, the display screen also has a second display state, and in the second display state, the display screen has The result display area of each detection point of the channel.
  • the multi-channel LAMP detector also includes a housing, and the housing is provided with a chip port for inserting a microfluidic chip, and each of the chip slots has a chip port corresponding to the chip port. Opposite notches; the multi-channel LAMP detector also includes a heating assembly for constant temperature heating of the microfluidic chip in the chip slot, and at least one The heating assembly; the multi-channel LAMP detector also includes a mechanical arm mechanism, the mechanical arm mechanism includes a plurality of mechanical arms that can engage with the pistons of the microfluidic chips in the chip chamber, each of the chips The slot corresponds to at least one of the mechanical arms, and each of the mechanical arms is movably arranged in the housing and located on the right side of the chip magazine.
  • the multi-channel LAMP detector also includes a door for closing the chip opening and a first driving mechanism for driving the door to move, and the door is movably arranged on the housing on the inner wall; and/or, the multi-channel LAMP detector further includes a chip cover, and the chip cover is arranged between the inner wall of the shell surrounding the chip opening and the chip chamber and between the chip slots.
  • the mechanical arm mechanism includes a bracket disposed on the inner wall of the housing, a lower mounting plate capable of being disposed on the bracket along the left-right direction, and an upper mounting plate capable of being moved along the left-right direction on the bracket.
  • the upper mounting plate is located above the lower mounting plate
  • the upper mounting plate is provided with a plurality of first mechanical arms
  • the lower mounting plate is provided with a plurality of second mechanical arms
  • each of the The chip slots are respectively corresponding to at least one of the first mechanical arms and at least one of the second mechanical arms.
  • the second mechanical arms are located at the lower left of the first mechanical arms.
  • first mechanical arm and/or the second mechanical arm respectively have engaging grooves for the piston of the microfluidic chip to snap into, and the engaging grooves have notches facing upward; and/or, the The mechanical arm mechanism also includes a second drive mechanism for driving the upper mounting plate to move left and right, and a third drive mechanism for driving the lower mounting plate to move left and right.
  • the second guide rail and the third guide rail, the upper mounting plate is arranged on the second guide rail so as to be movable in the left and right direction
  • the lower mounting plate is arranged on the third guide rail so as to be movable in the left and right direction
  • the The second drive mechanism includes a second motor and a second screw driven by the second motor
  • the second screw is connected to the upper mounting plate
  • the third drive mechanism includes a third motor and a second screw driven by the second motor.
  • the third motor drives a third lead screw that rotates, and the third lead screw is connected to the lower mounting plate; and/or, the mechanical arm mechanism also includes a function for detecting whether the first mechanical arm reaches a first detection switch for its initial position, and a second detection switch for detecting whether the second mechanical arm has reached its initial position; and/or, the bracket has a function for preventing the upper mounting plate and the lower The mounting plate moves to the right to a blocking portion beyond its set stroke.
  • the multi-channel LAMP detector also includes a main PCB board, and the housing is provided with a mounting frame between the display screen and the chip compartment, and the mounting frame is provided with A guide chute, the edge of the main PCB board is inserted into the guide chute.
  • a control method of the multi-channel LAMP detector as described above includes the following steps:
  • the controller After receiving the first detection signal and the third detection signal, the controller judges that the optical detection mechanism is at the origin, and detects the detection point P11 of the first microfluidic chip;
  • the controller controls the X-direction drive mechanism to run forward, so that the optical detection mechanism moves to the right for a set distance d1 and then stops to the detection point P12 for detection; and so on until the first micro The detection of all detection sites of the fluidic chip, at this time, the X-direction endpoint detection switch is triggered to send a second detection signal;
  • the controller controls the X-direction drive mechanism to run in reverse, so that the optical detection mechanism moves leftward to return to the origin, and at this time, the X-direction starting point detection switch is triggered and send out a first detection signal;
  • the controller controls the Y-direction drive mechanism to move backward by a set distance d2 and then stops to the detection point P21 of the second microfluidic chip for testing;
  • the controller controls the X-direction drive mechanism to run forward, so that the optical detection mechanism moves to the right for a set distance d1 and stops at the detection point P22 for detection; and so on until the second micro The detection of all detection sites of the fluidic chip, at this time, the X-direction endpoint detection switch is triggered to send a second detection signal;
  • the controller After the controller receives the second detection signal, it controls the X-direction driving mechanism to run in the reverse direction, so that the optical detection mechanism moves to the left until the X-direction starting point detection switch is triggered to send out the first detection Signal;
  • the controller controls the Y-direction drive mechanism to move backward to the detection point Pn1 of the last microfluidic chip for detection;
  • the controller controls the X-direction drive mechanism to run forward, so that the optical detection mechanism moves to the right for a set distance d1 and then stops to the detection point Pn2 for detection; and so on until the last micro-flow is completed.
  • the controller controls the X-direction drive mechanism to run in reverse, and moves the optical detection mechanism to the left until the X-direction starting point detection switch is triggered to send out the first detection signal, at this time, the Y-to-end detection switch is triggered to send a fourth detection signal;
  • the controller controls the Y-direction drive mechanism to run in reverse, so that the optical detection mechanism moves forward until the X-direction starting point detection switch and The Y-direction starting point detection switches are all triggered to send out the first detection signal and the third detection signal, and the Y-direction driving mechanism stops running.
  • control method also includes the following steps:
  • S201 Receive the user's first code scanning input instruction through the display screen, read the first barcode information of the first microfluidic chip through the code scanner, and the controller receives the first scanning code input instruction and After the first barcode information, associate the first barcode information with the first detection channel;
  • S202 Receive the user's second code scanning input instruction through the display screen, read the second barcode information of the second microfluidic chip through the code scanner, and the controller receives the second barcode information After scanning the code and inputting the instruction and the second barcode information, associating the second barcode information with the second detection channel;
  • the controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism and the detection point P11 of the first detection channel;
  • the controller sequentially establishes a corresponding relationship between the detection information sent by the optical detection mechanism and other detection points of the first detection channel;
  • the controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism and the detection point P21 of the second detection channel;
  • the controller sequentially establishes a corresponding relationship between the detection information sent by the optical detection mechanism and other detection points of the second detection channel;
  • the controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism and the detection point Pn1 of the last detection channel;
  • the controller sequentially establishes a corresponding relationship between the detection information sent by the optical detection mechanism and other detection points of the last detection channel.
  • the present invention adopts the above scheme, and has the following advantages compared with the prior art:
  • a kind of multi-channel LAMP detector of the present invention is provided with a plurality of chip slots in the chip chamber, and automatically detects all detection points of all detection channels by controlling the movement of the optical detection mechanism in the front, rear, left and right directions, It can process multiple microfluidic chips at one time, and the number of samples that can be processed once the detection program is run is greatly increased, with high detection efficiency; moreover, multiple detection channels of the LAMP detector can share an optical detection mechanism, Compared with the single-channel LAMP detector, the space is effectively used, the volume of the whole machine will not increase significantly, and it will not take up too much space.
  • Fig. 1 is the overall structure schematic diagram of a kind of multi-channel LAMP detector of the present embodiment
  • Fig. 2 is a schematic diagram of the internal structure of a multi-channel LAMP detector of the present embodiment
  • FIG. 3 is a schematic diagram of the internal structure of another viewing angle of a multi-channel LAMP detector of the present embodiment
  • FIG. 4 is an internal schematic diagram of a housing of a multi-channel LAMP detector of the present embodiment
  • FIG. 5 is a schematic structural diagram of the scanner bracket of this embodiment
  • FIG. 6 is a structural schematic diagram of another viewing angle of the scanner bracket of this embodiment.
  • FIG. 7 is a schematic structural view of the first heating module and the second heating module of this embodiment.
  • Fig. 8 is a structural schematic view of another viewing angle of the first heating module and the second heating module of this embodiment.
  • FIG. 9 is a schematic structural view of the mechanical arm mechanism of this embodiment.
  • Fig. 10 is another structural schematic diagram of the mechanical arm mechanism of this embodiment.
  • Fig. 11a is an axonometric view of the optical detection mechanism of this embodiment.
  • Fig. 11b is a top view of the optical detection mechanism of this embodiment.
  • FIG. 12 is a schematic structural view of the mounting frame of the present embodiment.
  • FIG. 14 is a schematic structural diagram of the chip cap of this embodiment.
  • FIG. 15a and FIG. 15b are schematic structural views of a chip socket in this embodiment
  • Fig. 16 is a schematic diagram of detection sites in this embodiment.
  • 501-code scanner 502-code scanner bracket; 5021-base; 5022-support table; 503-scanning window; 504-display screen;
  • 601-the first heating module 6011-the first insulation cotton compact; 6012-the first heating block; 6013-the first heating film; 6014-the first heat insulation sheet; 6015-the first insulation cotton; 602-the second heating Module; 6021-the second insulation cotton compact; 6022-the second heating block; 6023-the second heating film; 6024-the second heat insulation sheet; 6025-the second insulation cotton; 603-the first temperature sensor; 604-the first Two temperature sensors;
  • 801-X direction start detection switch 802-X direction end detection switch; 803-Y direction start detection switch; 804-Y direction end detection switch; 805-X direction motor; 806-Y direction motor; 809-X direction rail; 810-Y guide rail;
  • 101-mounting frame 101a-guiding chute.
  • a feature when a feature is called “fixed” or “connected” to another feature, it can be directly fixed and connected to another feature, or indirectly fixed and connected to another feature. on a feature.
  • first, second, etc. are used to describe various information, but the information should not be limited to these terms. These terms are only used to distinguish information of the same type from one another, and do not imply a specific order or degree of importance. In fact, expressions such as “first” and “second” can be used interchangeably.
  • first information may also be called second information, and similarly, second information may also be called first information.
  • FIG. 1 to FIG. 16 show a multi-channel LAMP detector according to this embodiment, which can automatically perform nucleic acid amplification detection based on the LAMP reaction.
  • the LAMP detector includes a housing 1 , an inner cavity for installing internal components is formed in the housing 1 , and a chip port 101 for inserting a microfluidic chip 100 is provided on the housing 1 .
  • the housing 1 is provided with a chip chamber 2 , and the chip chamber 2 has a plurality of chip slots 10 for accommodating microfluidic chips 100 . Specifically in this embodiment, there are three chip slots 10 inside the chip chamber 2, and each chip slot 10 corresponds to a detection channel.
  • a plurality of chip slots 10 are arranged side by side along the front and rear direction, and each chip slot 10 has a notch 102 opposite to the chip opening 101, specifically, the chip slot 10 is located directly below the chip opening 101 , the notch of the chip socket 10 faces upward, and the microfluidic chip 100 is inserted into the chip socket 10 from top to bottom.
  • the multi-channel LAMP detector further includes a door 3 movably arranged on the inner wall of the housing 1 , and the door 3 is used to close the chip opening 101 .
  • the door 3 is movably disposed on the inner wall of the upper side of the casing 1 .
  • a first guide rail 301 extending in the left-right direction is provided on the upper inner wall of the casing 1 , and the door 3 is movably arranged on the first guide rail 301 through a slider. The door 3 is driven to open and close by the first driving mechanism.
  • the first driving mechanism includes a first motor 302 and a first screw 303 driven to rotate by the first motor 302 , and the first screw 303 is connected to the door 3 through threads (for example, through a screw nut).
  • the first screw 303 can be driven by the first motor 302 to rotate, and through the cooperation of the screw and the screw nut, the rotational motion of the first motor 302 is converted into linear motion, so that the door 3 can move left and right.
  • the opening and closing of the door 3 is detected by the door sensor, the door sensor is electrically connected to a door controller, and the door controller is also electrically connected to the first motor 302; when the door sensor is triggered, a detection indicating that the door 3 is opened or closed is sent out After receiving the detection signal, the door controller sends a control signal to the first motor 302 to control its forward rotation or reverse rotation, thereby driving the door 3 to close or open.
  • the door controller is arranged on a door control PCB 93 , and the PCB 93 is arranged on the inner wall of the casing 1 . After the microfluidic chip is loaded, the door 3 is automatically closed to seal the chip port 101 to protect the internal components, which is more beautiful.
  • the multi-channel LAMP detector further includes a code scanner 501 for reading the barcode on the microfluidic chip 100 .
  • the code scanner 501 is installed in the housing 1 through the code scanner bracket 502 .
  • the housing 1 is provided with a scanning window 503 .
  • the scanning part of the code scanner 501 is located in the scanning window 503 or facing the scanning window 503 .
  • the scanner bracket 502 includes a base 5021 and a supporting platform 5022 arranged in parallel on the base 5021 .
  • the supporting platform 5022 is wedge-shaped.
  • a connecting hole 13 is also provided on the base 5021 , and the scanner bracket 502 is fixedly arranged on the inner wall of the housing 1 through a connecting piece passing through the connecting hole 13 .
  • Both the chip port 101 and the code scanner 501 are located on the upper side of the housing 1 and both face upward.
  • the multi-channel LAMP detector also includes a main controller and a display screen 504 (specifically, a touch screen), and the main controller is electrically connected to the code scanner 501 and the display screen 504 respectively.
  • the main controller is arranged on the main PCB board 91 .
  • the display screen 504 has a first display state, and in the first display state, the display screen 504 has a code scanning input button.
  • the information of the microfluidic chip 100 read by the code scanner 501 is associated with the first detection channel (for example, the chip slot 10 on the front side); After scanning the code input button, the information of the microfluidic chip 100 read by the code scanner 501 is associated with the second detection channel (for example, the chip slot 10 in the middle); after pressing the code scanning input button for the third time, The information of the microfluidic chip 100 read by the scanner 501 is associated with the third detection channel (eg, the last chip slot 10 ).
  • the display screen 504 is located on the left side of the housing 1 .
  • the multi-channel LAMP detector further includes a heating assembly for heating the microfluidic chip 100 in the chip slot 10 at a constant temperature.
  • the heating assembly is used to heat the reaction chamber of the microfluidic chip 100 .
  • Each chip socket 10 corresponds to at least one heating component, and the heating component is attached to the side wall of the chip socket 10 .
  • Each heating assembly includes a first heating module 601 and a second heating module 602, and the first heating module 601 includes a first thermal insulation cotton pressing block 6011, a first heating block 6012, a first heating film 6013, a first thermal insulation cotton 6015 and a first thermal insulation cotton 6015.
  • a thermal insulation sheet 6014 , the second heating module 602 includes a second thermal insulation cotton pressing block 6021 , a second heating block 6022 , a second heating film 6023 , a second thermal insulation cotton 6025 and a second thermal insulation sheet 6024 .
  • the structures of the first heating module 601 and the second heating module 602 are similar, and the difference is that the shapes of the second thermal insulation cotton pressing block 6021 and the second heating block 6022 of the second heating module 602 are similar to those of the first heating block 602 of the first heating module 601.
  • the shape of the thermal insulation cotton pressing block 6011 and the first heating block 6012 is different.
  • the first heating block 6012 and the first heat insulation sheet 6014 are covered and arranged on the first heating film 6013, the surface of the first heating film 6013 has an adhesive layer, and the first heating film 6013 is pasted together Covering and setting on the front of the first thermal insulation cotton 6015, the back of the first thermal insulation cotton 6015 is provided with a first thermal insulation cotton compact 6011, the first thermal insulation cotton compact 6011 has a connection hole 13, through the connection hole 13
  • the connecting member disposes the first heating module 601 on the sidewall of the chip socket 10 .
  • the first temperature sensor 603 is fixedly arranged on the first heating block 6012
  • the second temperature sensor 604 is fixedly arranged on the second heating block 6022.
  • the first temperature sensor 603 and the second temperature sensor 604 are connected with a controller (which can be the main controller) electrical connection.
  • the controller is used to correspondingly control the magnitude of the voltage supplied to the first heating film 6013 according to the temperature information detected by the first temperature sensor 603, thereby maintaining the heating temperature of the first heating block 6012 constant;
  • the temperature information detected by the second temperature sensor 604 controls the magnitude of the voltage supplied to the second heating film 6023 accordingly, so as to keep the heating temperature of the second heating block 6022 constant.
  • Fig. 15a and Fig. 15b show the partial structure of a single chip socket 10 in the chip chamber, in which the microfluidic chip 100 can be inserted.
  • a hollow area 10a is provided on the side wall of the chip socket 10, so as to cooperate with the heating assembly; Cooperate.
  • the first heating block 6012 matches the shape of a hollowed out area 10a on the upper part of the side wall of the chip socket 10, and is embedded in the hollowed out area 10a, so that it can be attached to the microfluidic chip in the chip socket 10; similarly
  • the second heating block 6022 matches the shape of a hollowed-out area 10a at the lower part of the side wall of the chip socket 10, and is embedded in the hollowed-out area 10a, so that it can be attached to the microfluidic chip in the chip socket 10.
  • the part that is not attached to the microfluidic chip is provided with a thermal insulation sheet, that is, the high-temperature component of the first heating module 601 is separated from the side wall of the chip socket 10 by the first thermal insulation sheet 6014, and the second insulation sheet 6014
  • the thermal sheet 6024 separates the high-temperature components of the second heating module 602 from the sidewall of the chip socket 10 to avoid the problem of premature aging caused by the chip socket 10 being exposed to a high-heat environment for a long time.
  • the multi-channel LAMP detector also includes a mechanical arm mechanism 7, the mechanical arm mechanism 7 includes a plurality of mechanical arms that can engage with the piston 100a of the microfluidic chip 100 in the chip warehouse 2, and the mechanical arm Used to drive the piston 100a to move.
  • Each chip slot 10 corresponds to at least one mechanical arm, and each mechanical arm is arranged in the casing 1 and is located on the right side of the chip magazine 2 so as to be movable in the left and right directions.
  • the mechanical arm mechanism 7 includes a bracket 701 arranged on the inner wall of the housing 1, an upper mounting plate 702 that can be moved along the front and rear directions on the bracket 701, and a lower mounting plate 703 that can be arranged on the bracket 701 along the left and right directions.
  • the 702 is located above the lower mounting plate 703.
  • the upper mounting plate 702 is provided with a plurality of first mechanical arms 704, and the lower mounting plate 703 is provided with a plurality of second mechanical arms 705.
  • Each chip slot 10 is respectively connected to at least one of the first mechanical arms.
  • a robot arm 704 and at least one second robot arm 705 correspond to each other. In the initial position, the second robot arm 705 is located at the lower left of the first robot arm 704 .
  • the first mechanical arm 704 and the second mechanical arm 705 respectively have an end portion (specifically the neck 100a1 of the piston 100a) of the microfluidic chip 100 that can be snapped into
  • the engaging groove 706 has the notch 102 facing upward.
  • the first mechanical arm 704 and the second mechanical arm 705 are spaced apart from each other in front, rear, left and right, and can be combined with two pistons 100a at the same time.
  • the second mechanical arm 705 is located at the lower rear of the first mechanical arm 704 , that is, the second mechanical arm 705 is located at a distance from the rear side of the first mechanical arm 704 to match the positions of the two pistons 100 a.
  • the mechanical arm mechanism 7 also includes a second drive mechanism for driving the upper mounting plate 702 to move left and right, and a third drive mechanism for driving the lower mounting plate 703 to move left and right.
  • the upper mounting plate 702 is arranged on the second guide rail 709 to be movable in the left-right direction, and the lower mounting plate 703 is movable in the left-right direction.
  • the second driving mechanism includes a second motor 7071 and a second screw 7072 driven by the second motor 7071, the second screw 7072 is connected to the upper mounting plate 702, and the third driving mechanism includes the second Three motors 7081 and a third lead screw (not shown) driven by the third motor 7081 to rotate, the third lead screw is connected with the lower mounting plate 703 .
  • the mechanical arm mechanism 7 also includes a first photoelectric switch 711 for detecting whether the first mechanical arm 704 has reached its initial position, and a second photoelectric switch 712 for detecting whether the second mechanical arm 705 has reached its initial position.
  • the bracket 701 has a blocking portion 7011 for preventing the upper mounting plate 702 and the lower mounting plate 703 from moving rightward beyond their set stroke.
  • the second motor 7071 and the third motor 7081 are also respectively electrically connected to the controller of the robotic arm.
  • the controller of the robotic arm is used to send control signals to control the operation of the second motor 7071 and the third motor 7081 respectively.
  • a photoelectric switch 711 is electrically connected to the second photoelectric switch 712 to receive signals from the first photoelectric switch 711 and the second photoelectric switch 712 .
  • the robotic arm controller is located on the robotic arm control PCB 92 . This embodiment can precisely control the first mechanical arm 704 and the second mechanical arm 705, the three first mechanical arms 704 are controlled by the same drive system, and the three second mechanical arms 705 are controlled by the same drive system, which can synchronously control the three micro
  • the movement of the piston of the fluidic chip 100 has high control precision and avoids excessive cost increase.
  • the controller of the mechanical arm will send a control signal to the second motor 7071 to make the second motor 7071 start to run.
  • the second motor 7071 drives the second screw rod 7072 to rotate and drives the upper mounting plate 702 to move linearly.
  • the upper mounting plate 702 is provided with a first mechanical arm 704 , and the first mechanical arm 704 will move linearly along with the upper mounting plate 702 .
  • the first mechanical arm 704 is provided with a first photoelectric switch sensing bracket 7041, and the first photoelectric switch sensing bracket 7041 will move linearly along the second guide rail 709 with the first mechanical arm 704 until the first photoelectric switch sensing bracket 7041 moves to At the first photoelectric switch 711, the first photoelectric switch 711 is triggered to send a detection signal, which is transmitted to the controller of the robotic arm. After receiving the detection signal, the controller of the robotic arm controls the second motor 7071 to run in reverse or stop.
  • the second motor 7071 When the second motor 7071 is running, the second screw 7072 moves forward or backward along the front-back direction, and the first mechanical arm 704 moves forward or backward along the second guide rail 709, thereby driving the upper part of the microfluidic chip 100
  • the piston 100a moves left and right, and then provides positive or negative pressure to the liquid in the microfluidic chip 100, thereby providing power for liquid circulation.
  • the controller of the robotic arm sends a control signal to the third motor 7081 to make the third motor 7081 start to run.
  • the third motor 7081 drives the third screw to rotate and drives the lower mounting plate 703 to move linearly.
  • a second mechanical arm 705 is disposed on the lower mounting plate 703 , and the second mechanical arm 705 will move linearly along with the lower mounting plate 703 .
  • the second mechanical arm 705 is provided with a second photoelectric switch induction bracket 7051 .
  • the second photoelectric switch sensing bracket 7051 will move linearly along the third guide rail with the second mechanical arm 705 until the second photoelectric switch sensing bracket 7051 moves to the second photoelectric switch 712, and the second photoelectric switch 712 is triggered to send a detection
  • the signal is transmitted to the controller of the robotic arm.
  • the controller of the robotic arm controls the third motor 7081 to run in reverse or to stop running.
  • the third motor 7081 is running, the third screw rod moves left or right along the left and right directions, and the second mechanical arm 705 moves left or right along the third guide rail, thereby driving the lower piston of the microfluidic chip 100 100a moves left and right, and then provides positive pressure or negative pressure to the liquid in the microfluidic chip 100 so as to provide power for liquid circulation, or switch on and off between chambers in the microfluidic chip 100 .
  • the multi-channel LAMP detector includes an optical detection mechanism 8, the optical detection mechanism 8 can be moved along the left and right direction and the front and rear directions in the housing 1, the optical detection mechanism 8 is located in the chip chamber 2 Below, each amplification detection chamber of the microfluidic chip 100 in each chip slot 10 is detected one by one in order, wherein a plurality of microfluidic chips 100 are arranged along the front and rear directions, and each microfluidic chip 100 has a A plurality of amplification detection chambers arranged at intervals in the direction, each amplification detection chamber is a detection site, as shown in FIG. 16 .
  • the optical detection mechanism 8 moves along the left and right direction, and can scan multiple detection sites of the microfluidic chip 100 in a certain chip slot 10 one by one; It is aligned with another microfluidic chip 100 , and then moves in the left and right directions to scan the multiple detection sites of the microfluidic chip 100 one by one.
  • the multi-channel LAMP detector also includes a base 11 arranged on the inner wall of the housing 1, the base 11 has an X guide rail 809 extending in the left and right directions; the multi-channel LAMP detector also includes a device capable of moving in the left and right directions.
  • the multi-channel LAMP detector also includes an X-direction driving mechanism for driving the mounting block to move along the X-guiding rail 809, a Y-direction driving mechanism for driving the optical detection mechanism 8 to move along the Y-guiding rail 810, and a Y-direction driving mechanism for detecting the optical detection mechanism 8 to move along the Y-guiding rail 810.
  • the X-direction drive mechanism includes an X-direction motor 805, and the X-direction motor 805 is arranged on the base 11 and drives the Y guide rail 810 to move left and right through a screw and a nut;
  • the Y-direction drive mechanism includes a Y-direction motor 806 , the Y-direction motor 806 is arranged on the mounting block and drives the optical detection mechanism 8 to move forward and backward along the Y-direction rail through a screw rod and a nut.
  • the X-direction start detection switch 801 , the X-direction end detection switch 802 , the Y-direction start detection switch 803 , and the Y-direction end detection switch 804 are photoelectric detection switches, respectively. Referring to Fig. 16, the X-direction start detection switch 801 sends out a first detection signal when it detects that the optical detection mechanism 8 reaches its starting point in the left-right direction, and the X-direction end detection switch 802 is used to detect that the optical detection mechanism 8 arrives.
  • the optical detection mechanism 8 has a plurality of detection positions, and each chip socket 10 has a plurality of detection positions arranged along the left and right directions, and each detection position of each chip socket 10 corresponds to a detection position.
  • the microfluidic chip in each chip slot 10 corresponds to one detection channel, a total of three detection channels C1, C2 and C3, and each chip slot 10 has four detection sites, there are three chip slots 10, so there are twelve detection sites; specifically, detection channel C1 has four detection sites P11, P12, P13 and P14, and detection channel C2 has four detection sites Sites P21, P22, P23 and P24; detection channel C3 has four detection sites P31, P32, P33 and P34.
  • the optical detection mechanism 8 starts to run from the origin, and moves according to the coordinates formed by the detection points. After the optical detection mechanism 8 passes through four detection points, the optical detection mechanism 8 Move to the next chip socket 10 along the Y direction, detect the second chip socket 10, and so on.
  • the main controller is electrically connected to the X-direction starting point detection switch 801, the X-direction end point detection switch 802, the Y-direction starting point detection switch 803, and the Y-direction end point detection switch 804, and controls the X-direction driving mechanism to move forward after receiving the first detection signal.
  • control the X-direction drive mechanism to run in reverse
  • control the Y-direction drive mechanism to run forward
  • the Y-direction driving mechanism is controlled to run in reverse.
  • the housing 1 is provided with a mounting frame 101 , and the mounting frame 101 is located between the display screen 504 and the chip compartment 2 .
  • the installation frame 101 is provided with a guide chute 101a, and the edge of the main PCB board 91 is inserted into the guide chute 101a.
  • the PCB board can be installed in a simple structure and low-cost manner, the volume is small, and the installation is convenient, especially in the case of a relatively narrow installation space, the assembly and maintenance are relatively convenient.
  • the multi-channel LAMP detector also includes a chip cover 4, and the chip cover 4 is arranged between the inner wall of the housing 1 around the chip port 101 and the chip chamber 2 and between the chip slots 10, and its hollowed out part and The slots of each chip socket 10 correspond to allow the microfluidic chip 100 to be inserted into the chip socket 10 .
  • the chip cover 4 is used to seal the gap between the chip chamber 2 and the inner wall of the housing 1 and the gap between the chip slots 10 to prevent foreign matter from falling in, prevent dust, and protect internal components.
  • the chip cover 4 can also play a role of shielding the parts inside the tester, which is more beautiful.
  • the chip cap 4 adopts 3D printing technology, which has a simple structure and can solve the problem of high manufacturing cost.
  • the door 3 After aligning the microfluidic chip 100 with the code scanner 501 and scanning the code, insert it into the chip slot 10, the door 3 is automatically closed, and the first mechanical arm 704 and the second mechanical arm 705 of the mechanical arm mechanism 7 follow the set sequence. Move, drive the piston 100a to move, communicate with the cavity, and provide positive or negative pressure for fluid flow, so that the sample and reagent of the microfluidic chip 100 are mixed or the reagent is circulated into the target chamber until it enters the reaction chamber for further processing.
  • Amplification reaction power on the heating component to heat the corresponding chamber of the microfluidic chip 100; after the reaction, the optical detection mechanism 8 is powered on, and moves to each detection position in sequence according to the set time sequence, for each An amplification detection chamber for optical detection.
  • the present embodiment also provides a control method of a multi-channel LAMP detector, which includes the following process:
  • process D specifically includes the following steps:
  • the optical detection mechanism 8 is at the origin position or moved to the origin position (that is, the detection point P11), the X-direction starting point detection switch 801 and the Y-direction starting point detection switch 803 are both triggered to send out the first detection signal and the third detection signal, after receiving the first detection signal and the third detection signal, the main controller judges that the optical detection mechanism 8 is at the origin, and detects the detection point P11 of the first microfluidic chip 100 .
  • the main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P11 of the first detection channel C1.
  • the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves to the right for a set distance d1 and then stops to the detection point P12 for detection; specifically, by setting the distance d1 (that is, two adjacent distance between detection points) to convert the rotation angle of the X-direction motor 805, and stop when the X-direction motor 805 turns over the set angle.
  • the main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P12 of the first detection channel C1.
  • control the optical detection mechanism 8 to sequentially move to the detection positions P13 and P14 for detection, and the main controller sequentially establishes the detection information sent by the optical detection mechanism 8 with the detection positions P13 and P14 of the first detection channel C1 corresponding relationship.
  • the X-direction endpoint detection switch 802 is triggered to send a second detection signal.
  • the main controller After the main controller receives the second detection signal and waits for the set time, it controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves leftward to return to the origin, and at this time the X-direction starting point detection switch 801 is triggered
  • the first detection signal is issued; the set time is at least longer than the time required by the optical detection mechanism 8 to complete the optical detection of one detection point.
  • the main controller receives the first detection signal and the third detection signal, controls the Y-direction motor 806 to move backward by a set distance d2, and then stops the detection of the second microfluidic chip 100
  • the main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P21 of the second detection channel C2.
  • the rotation angle of the Y-direction motor 806 is converted by setting the distance d2 (that is, the distance between two adjacent microfluidic chips 100), and the Y-direction motor 806 stops when it rotates through the set angle.
  • the main controller controls the X-direction motor 805 to run forward, so that the optical detection mechanism 8 moves to the right for a set distance d1 and then stops to the detection position P22 for detection;
  • the information establishes a corresponding relationship with the detection point P22 of the second detection channel C2.
  • the optical detection mechanism 8 is controlled to move to the detection positions P23 and P24 in turn for detection, and the main controller sequentially establishes the detection information sent by the optical detection mechanism 8 with the detection positions P23 and P24 of the second detection channel C2. corresponding relationship.
  • the X-direction endpoint detection switch 802 is triggered to send a second detection signal.
  • the main controller controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves to the left until the X-direction starting point detection switch 801 is triggered to send out the first detection signal.
  • a heartbeat After receiving the second detection signal and waiting for the set time, the main controller controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves to the left until the X-direction starting point detection switch 801 is triggered to send out the first detection signal. A heartbeat.
  • the main controller receives the first detection signal, and after the main controller receives the first detection signal, controls the Y-direction motor 806 to move backward d2 to the detection of the last microfluidic chip 100
  • the main controller establishes a corresponding relationship between the detection information sent by the optical detection mechanism 8 at this time and the detection point P31 of the third detection channel C3.
  • the optical detection mechanism 8 is controlled to move to the detection positions P33 and P34 in sequence for detection, and the main controller sequentially establishes the detection information sent by the optical detection mechanism 8 with the detection positions P33 and P34 of the third detection channel C3. corresponding relationship.
  • the X-direction endpoint detection switch 802 is triggered to send a second detection signal.
  • the main controller controls the X-direction motor 805 to run in reverse, so that the optical detection mechanism 8 moves to the left until the X-direction starting point detection switch 801 is triggered to send out The first detection signal; meanwhile, in the process of detecting the third detection channel, the Y-to-end detection switch 804 is triggered to send a fourth detection signal;
  • the main controller After the main controller receives the first detection signal and the fourth detection signal, it controls the Y direction motor 806 to run in reverse, so that the optical detection mechanism 8 moves forward until the X direction starting point detection switch 801 and the Y direction starting point detection switch 803 are both Triggered to send out the first detection signal and the third detection signal, the Y-direction motor 806 stops running, so that the optical detection mechanism 8 returns to the origin and waits for the next detection.
  • Process A specifically includes:
  • the above-mentioned barcode information includes the information of the sample to be tested, such as serial number, corresponding subject's name, source and so on.
  • the display screen 504 is switched to the second display state, and the detection results (yin/yang) of each detection site, the associated number, the name of the subject, etc. are displayed.
  • the chip chamber 2 is provided with a plurality of chip slots 10, which can detect a plurality of chips at the same time, and can process a plurality of microfluidic chips 100 at one time.
  • the detection procedure The number of samples that can be processed in one operation is greatly improved, and the detection efficiency is high; the door 3 and the chip cover 4 can prevent dust and foreign matter from entering the gap between the chip chamber 2 and the inner wall, and the chip cover 4 can also It can shield the components inside the detector and make the appearance more beautiful; the multiple detection channels of the LAMP detector can share some components, such as the mechanical arm mechanism, optical detection mechanism 8, etc.
  • the volume of the whole machine will not increase significantly, and no additional space will be taken up.
  • the volume of the detector is still similar to that of a single-channel detector, and the structure is more compact, the space is optimized, the volume is small, the operation is simple, the use is convenient, and the cost is saved.

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Abstract

一种多通道LAMP检测仪及其控制方法，检测仪包括芯片仓(2)和光学检测机构(8)，芯片仓(2)具有多个芯片插槽(10)；光学检测机构(8)能够沿左右方向及前后方向移动地设置于壳体(1)内，光学检测机构(8)位于芯片仓(2)的下方；各芯片插槽(10)具有沿左右方向排列的多个检测位点(P11,P12,P13,P14,P21,…,P24,P31, …,P34)，每个芯片插槽(10)的每个检测位点(P11,P12,P13,P14,P21,…,P24,P31,…, P34)分别对应一个检测位置；检测仪还包括：X向驱动机构，用于驱动光学检测机构(8)沿左右方向移动；Y向驱动机构，用于驱动光学检测机构(8)沿前后方向移动；控制器，用于接收X向起点检测开关(801)、X向终点检测开关(802)、Y向起点检测开关(803)及Y向终点检测开关(804)的检测信号，并控制X向驱动机构和Y向驱动机构。检测仪能够一次对多个微流控芯片(100)中的样本进行处理，同时体积较小。

Description

一种多通道LAMP检测仪及其控制方法
优先权
本申请要求2021年9月28日提交的申请号为CN 2021111414195的中国专利申请的优先权。
技术领域
本发明属于核酸检测技术领域，涉及一种多通道LAMP检测仪及其控制方法。
背景技术
LAMP(环介导等温扩增，Loop-mediated isothermal amplification)技术因其反应条件温和(反应温度较低)、反应时间短等优点广泛应用于生物诊断领域，如进行核酸扩增检测以诊断样本中是否存在病原体。目前，LAMP技术是通过为核酸片段提供体外扩增的条件，使之呈指数大量扩增并在核酸扩增过程中加入荧光染料或荧光标记物，采用光学装置检测出荧光信号的强弱，通过对荧光信号的分析得出核酸扩增结果的过程。在进行核酸扩增反应时，需要对反应体系进行加热。目前的LAMP检测仪能够集核酸扩增检测于一体，当检测芯片(通常为微流控芯片)放入LAMP检测仪后，能够对检测芯片的反应腔(通常为扩增反应腔)进行加热、光照、检测等。但当待检测的样本量较大时，就需要一种能够一次检测多个样品的LAMP检测仪。
发明内容
鉴于上述技术问题，本发明的一个目的提供一种多通道LAMP检测仪，其能够一次对多个微流控芯片中的样本自动进行检测，提高检测效率，同时体积较小。
本发明的另一个目的是提供一种多通道LAMP检测仪的控制方法，其在不额外增加器件成本的前提下一次对多个微流控芯片的样本自动进行检测，提高检测效率。
根据本发明的第一个方面，一种多通道LAMP检测仪，包括芯片仓和光学检测机构，所述芯片仓具有多个用于容置微流控芯片的芯片插槽，多个所述芯片插槽沿前后方向并列设置；所述光学检测机构能够沿左右方向及前后方向移动地设置于所述壳体内，所述光学检测机构位于所述芯片仓的下方；各所述芯片插槽分别具有沿左右方向排列的多个检测位点，所述光学检测机构具有多个检测位置，每个所述芯片插槽的每个所述检测位点分别对应一个所述检测位置；所述多通道LAMP检测仪还包括：
X向驱动机构，其用于驱动所述光学检测机构沿左右方向移动；
Y向驱动机构，其用于驱动所述光学检测机构沿前后方向移动；
X向起点检测开关，其用于在检测到所述光学检测机构到达其在左右方向上的起点时发出第一检测信号；
X向终点检测开关，其用于在检测到所述光学检测机构到达其在左右方向上的终点时发出第二检测信号；
Y向起点检测开关，其用于在检测到所述光学检测机构到达其在前后方向上的起点的第三检测信号；
Y向终点检测开关，其用于在检测所述光学检测机构到达其在前后方向上的终点的第四检测信号；
控制器，其用于接收所述X向起点检测开关、X向终点检测开关、Y向起点检测开关及Y向终点检测开关的检测信号，并能够在接收到所述第一检测信号后控制所述X向驱动机构正向运行，及能够在接收到所述第二检测信号后控制所述X向驱动机构反向运行，及能够在接收到所述第三检测信号后控制所述Y向驱动机构正向运行，及能够在接收到所述第四检测信号后控制所述Y向驱动机构反向运行。
在一优选的实施例中，所述控制器，用于在接收到所述第一检测信号和所述第三检测信号时，控制所述X向驱动驱动机构正向运行第一设定距离后停止设定时间，重复该过程一或多次，直至接收到所述第二检测信号后，控制所述X向驱动机构反向运行；直至再次接收到所述第一检测信号后，控制所述X向驱动机构停止运行。
在一优选的实施例中，所述控制器，还用于在接收到所述第一检测信号和所述第三检测信号时控制所述Y向驱动机构正向运行第二设定距离后停止设定时间；控制所述X向驱动驱动机构正向运行第一设定距离后停止设定时间，重复该过程一或多次，直至接收到所述第二检测信号后，控制所述X向驱动机构反向运行；直至再次接收到所述第一检测信号后，控制所述X向驱动机构停止运行。
在一优选的实施例中，所述控制器，还用于在接收到所述第一检测信号时控制所述Y向驱动机构正向运行，直至接收到所述第四检测信号，控制所述Y向驱动机构停止运行；控制所述X向驱动驱动机构正向运行第一设定距离后停止设定时间，重复该过程一或多次，直至接收到所述第二检测信号后，控制所述X向驱动机构反向运行；直至再次接收到所述第一检测信号后，控制所述X向驱动机构停止运行。
在一优选的实施例中，所述控制器，还用于在接收到所述第四检测信号和所述第一检测信号时控制所述Y向驱动机构反向运行，直至接收到所述第三检测信号，控制所述Y向驱动机构停止运行。
更优选地，所述设定时间相等。
在一优选的实施例中，，所述X向驱动机构包括X向电机，所述Y向驱动机构包括Y向电机，所述控制器分别和所述X向电机及所述Y向电机电性连接。
更优选地，所述多通道LAMP检测仪包括底座，所述底座具有多个并列设置且沿左右方向延伸的X向导轨；所述多通道LAMP检测仪还包括能够沿左右方向移动地设于所述X向导轨上的安装块，所述安装块上设有沿前后方向延伸的Y向导轨；所述光学检测机构能够沿前后方向移动地设于所述Y向导轨上；所述X向电机设于所述底座上并通过X向丝杆连接于所述安装块或所述Y向导轨，所述Y向电机设于所述安装块上并通过Y向丝杆连接于所述光学检测机构；所述X向起点检测开关和所述X向终点检测开关分别靠近所述X向导轨的两端部，所述Y向起点检测开关和所述Y向终点检测开关分别靠近所述Y向导轨的两端部。
在一优选的实施例中，所述控制器分别和所述X向起点检测开关、X向终点检测开关、Y向起点检测开关及Y向终点检测开关电性连接
在一优选的实施例中，所述多通道LAMP检测仪还包括用于读取微流控芯片上的条码的扫码器、及用作人机交互界面的显示屏，所述显示屏具有第一显示状态，在所述第一显示状态时，所述显示屏具有用于供用户输入扫码录入指令的扫码录入按钮，所述控制器还用于在接收到所述扫码录入指令后按照设定次序将所述扫码器读取的条码信息和对应的检测通道建立对应关系，每个检测通道对应一个所述芯片插槽。
更优选地，所述控制器还用于接收所述光学检测机构返回的检测数据，所述显示屏还具有第二显示状态，在所述第二显示状态时，所述显示屏具有对应各个检测通道的各个检测位点的结果显示区域。
在一优选的实施例中，所述多通道LAMP检测仪还包括壳体，所述壳体上设有供微流控芯片插入的芯片口，各所述芯片插槽分别具有与所述芯片口相对的槽口；所述多通道LAMP检测仪还包括用于对所述芯 片槽内的微流控芯片进行恒温加热的加热组件，每个所述芯片插槽的侧壁上分别设有至少一个所述加热组件；所述多通道LAMP检测仪还包括机械臂机构，所述机械臂机构包括多个能够和所述芯片仓中的微流控芯片的活塞接合的机械臂，每个所述芯片插槽对应至少一个所述机械臂，各所述机械臂能够沿左右方向移动地设置于所述壳体内并位于所述芯片仓的右侧。
更优选地，所述多通道LAMP检测仪还包括用于将所述芯片口封闭的门及用于驱动所述门移动的第一驱动机构，所述门可移动地设置于所述壳体的内壁上；和/或，所述多通道LAMP检测仪还包括芯片罩，所述芯片罩设于围绕所述芯片口的壳体内壁和所述芯片仓之间以及所述芯片槽之间。
更优选地，所述机械臂机构包括设置于所述壳体内壁的支架、能够沿左右方向设置于所述支架上的下安装板及能够沿左右方向移动地设置于所述支架上的上安装板，所述上安装板位于所述下安装板的上方，所述上安装板上设有多个第一机械臂，所述下安装板上设置有多个第二机械臂，每个所述芯片槽分别与至少一个所述第一机械臂及至少一个所述第二机械臂对应，在初始位置时，所述第二机械臂位于所述第一机械臂的左下方。
进一步地，所述第一机械臂和/或所述第二机械臂分别具有可供微流控芯片的活塞卡入的接合槽，所述接合槽具有面向上方的槽口；和/或，所述机械臂机构还包括用于驱动所述上安装板左右移动的第二驱动机构、及用于驱动所述下安装板左右移动的第三驱动机构，所述支架上设置有沿左右方向延伸的第二导轨和第三导轨，所述上安装板能够沿左右方向移动地设置于所述第二导轨上，所述下安装板能够沿左右方向移动地设置于所述第三导轨上，所述第二驱动机构包括第二电机及由所述第二电机驱动转动的第二丝杆，所述第二丝杠和所述上安装板相连接，所述第三驱动机构包括第三电机及由所述第三电机驱动转动的第三丝杠，所述第三丝杆和所述下安装板相连接；和/或，所述机械臂机构还包括用于检测所述第一机械臂是否到达其初始位置的第一检测开关、及用于检测所述第二机械臂是否到达其初始位置的第二检测开关；和/或，所述支架具有用于阻止所述上安装板和所述下安装板向右移动至其设定行程之外的阻挡部。
在一优选的实施例中，所述多通道LAMP检测仪还包括主PCB板，所述壳体内设有位于所述显示屏和所述芯片仓之间的安装架，所述安装架上设有导向滑槽，所述主PCB板的边缘部位插设于所述导向滑槽中。
根据本发明的第二个方面，一种如上所述的多通道LAMP检测仪的控制方法，所述控制方法包括如下步骤：
S101、控制器接收到第一检测信号和第三检测信号后，判断所述光学检测机构处于原点，并对第一个微流控芯片的检测位点P11进行检测；
S102、控制器控制所述X向驱动机构正向运行，使所述光学检测机构向右移动设定距离d1后停止到检测位点P12以进行检测；以此类推，直至完成对第一个微流控芯片的所有检测位点的检测，此时所述X向终点检测开关被触发而发出第二检测信号；
S103、控制器接收到所述第二检测信号后，控制所述X向驱动机构反向运行，使所述光学检测机构向左移动至回到原点，此时所述X向起点检测开关被触发而发出第一检测信号；
S104、控制器接收到所述第一检测信号和所述第三检测信号后，控制所述Y向驱动机构向后移动设定距离d2后停止到第二个微流控芯片的检测位点P21以进行检测；
S105、控制器控制所述X向驱动机构正向运行，使所述光学检测机构向右移动设定距离d1后停止到检测位点P22以进行检测；以此类推，直至完成对第二个微流控芯片的所有检测位点的检测，此时所述X向终点检测开关被触发而发出第二检测信号；
S106、控制器接收到所述第二检测信号后，控制所述X向驱动机构反向运行，使所述光学检测机构向左移动，直至所述X向起点检测开关被触发而发出第一检测信号；
S107、控制器接收到所述第一检测信号后，控制所述Y向驱动机构向后移动至最后一个微流控芯片的检测位点Pn1以进行检测；
S108、控制器控制所述X向驱动机构正向运行，使所述光学检测机构向右移动设定距离d1后停止到检测位点Pn2以进行检测；以此类推，直至完成对最后一个微流控芯片的所有检测位点的检测，此时所述X向终点检测开关被触发而发出第二检测信号；
S109、控制器接收到所述第二检测信号后，控制所述X向驱动机构反向运行，使所述光学检测机构向左移动，直至所述X向起点检测开关被触发而发出第一检测信号，此时所述Y向终点检测开关被触发而发出第四检测信号；
S110、控制器接收到所述第一检测信号和所述第四检测信号后，控制所述Y向驱动机构反向运行，使所述光学检测机构向前移动直至所述X向起点检测开关和所述Y向起点检测开关均被触发而发出第一检测信号和第三检测信号，所述Y向驱动机构停止运行。
在一优选的实施例中，所述控制方法还包括如下步骤：
S201、通过显示屏接收用户的第一次扫码输入指令，通过扫码器读取第一个微流控芯片的第一条码信息，控制器在接收到所述第一次扫码输入指令和所述第一条码信息后，将所述第一条码信息和第一个检测通道相关联；
S202、通过所述显示屏接收用户的第二次扫码输入指令，通过所述扫码器读取第二个微流控芯片的第二条码信息，所述控制器在接收到所述第二次扫码输入指令和所述第二条码信息后，将所述第二条码信息和第二个检测通道相关联；
以此类推，直至将所有的检测通道和微流控芯片的条码信息对应关联。
更优选地，所述步骤S101中，所述控制器将所述光学检测机构发送的检测信息与第一个检测通道的检测位点P11建立对应关系；
所述步骤S102中，所述控制器将所述光学检测机构发送的检测信息与第一个检测通道的其他检测位点依次建立对应关系；
所述步骤S104中，所述控制器将所述光学检测机构发送的检测信息与第二个检测通道的检测位点P21建立对应关系；
所述步骤S105中，所述控制器将所述光学检测机构发送的检测信息与第二个检测通道的其他检测位点依次建立对应关系；
所述步骤S107中，所述控制器将所述光学检测机构发送的检测信息与最后一个检测通道的检测位点Pn1建立对应关系；
所述步骤S108中，所述控制器将所述光学检测机构发送的检测信息与最后一个检测通道的其他检测位点依次建立对应关系。
本发明采用以上方案，相比现有技术具有如下优点：
本发明的一种多通道LAMP检测仪，在芯片仓内设有多个芯片插槽，通过控制光学检测机构在前后、 左右方向上的移动来对所有检测通道的所有检测位点自动进行检测，能够一次对多个微流控芯片进行处理，检测程序运行一次所能处理的样本数量大大提高，具有较高的检测效率；而且，该LAMP检测仪的多个检测通道可以共用一个光学检测机构，相比单通道的LAMP检测仪，有效利用了空间，整机体积并不会明显增加，不会额外占用过多空间。
附图说明
为了更清楚地说明本发明的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1为本实施例的一种多通道LAMP检测仪的整体结构示意图；
图2为本实施例的一种多通道LAMP检测仪的内部结构示意图；
图3为本实施例的一种多通道LAMP检测仪的另一视角的内部结构示意图；
图4为本实施例的一种多通道LAMP检测仪的壳体的内部示意图；
图5为本实施例的扫码器支架的结构示意图；
图6为本实施例的扫码器支架的另一视角的结构示意图；
图7为本实施例的第一加热模块和第二加热模块的结构示意图；
图8为本实施例的第一加热模块和第二加热模块的另一视角的结构示意图；
图9为本实施例的机械臂机构的结构示意图；
图10为本实施例的机械臂机构的另一结构示意图；
图11a为本实施例的光学检测机构的轴侧图；
图11b为本实施例的光学检测机构的俯视图；
图12为本实施例的安装架的结构示意图；
图13为本实施例的LAMP检测仪所配套的微流控芯片的结构示意图；
图14为本实施例的芯片罩的结构示意图；
图15a和图15b分别为本实施例的一芯片插槽的结构示意图；
图16为本实施例的检测位点示意图。
其中，
1-壳体；2-芯片仓；3-门；4-芯片罩；6-加热组件；7-机械臂机构；8-光学检测机构；10-芯片插槽；11-底座；12-底脚；13-连接孔；
100-微流控芯片；100a-活塞；100a1-颈部；
101-芯片口；102-槽口；
301-第一导轨；302-第一电机；303-第一丝杆；
501-扫码器；502-扫码器支架；5021-基座；5022-支撑台；503-扫描窗口；504-显示屏；
601-第一加热模块；6011-第一保温棉压块；6012-第一加热块；6013-第一加热膜；6014-第一隔热片；6015-第一保温棉；602-第二加热模块；6021-第二保温棉压块；6022-第二加热块；6023-第二加热膜；6024-第二隔热片；6025-第二保温棉；603-第一温度传感器；604-第二温度传感器；
701-支架；7011-阻挡部；702-上安装板；703-下安装板；704-第一机械臂；7041-第一光电开关感应支架；705-第二机械臂；7051-第二光电开关感应支架；706-接合槽；7071-第二电机；7072-第二丝杆；7081-第三电机；709-第二导轨；711-第一光电开关；712-第二光电开关；
801-X向起点检测开关；802-X向终点检测开关；803-Y向起点检测开关；804-Y向终点检测开关；805-X向电机；806-Y向电机；809-X向导轨；810-Y向导轨；
91-主PCB板；92-机械臂控制PCB板；93-门控制PCB板；
101-安装架；101a-导向滑槽。
具体实施方式
下面结合附图对本发明的较佳实施例进行详细阐述，以使本发明的优点和特征能更易于被本领域的技术人员理解。在此需要说明的是，对于这些实施方式的说明用于帮助理解本发明，但并不构成对本发明的限定。
在本申请的描述中，需要理解的是，术语“上”、“下”“左”、“右”、“前”、“后”、“竖直”、“水平”、“内”以及“外”等指示的方位或位置关系为基于附图1和2所示的方位或位置关系，仅是为了便于描述本申请和简化描述，而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本申请的限制。
如本说明书和权利要求书中所示，术语“包括”与“包含”仅提示包括已明确标识的步骤和元素，而这些步骤和元素不构成一个排它性的罗列，方法或者设备也可能包含其他的步骤或元素。本文所使用的术语“和/或”包括一个或多个相关的所列项目的任意的组合。
需要说明的是，如无特殊说明，当某一特征被称为“固定”、“连接”在另一个特征，它可以直接固定、连接在另一个特征上，也可以间接地固定、连接在另一个特征上。
除非上下文明确说明，否则位于本文的元素或成份之前的不定冠词「一」、「一个」及「一种」应理解为包括一个或至少一个，且所述元素或成分的单数词形也包括复数形式。
进一步可以理解的是，术语“第一”、“第二”等用于描述各种信息，但这些信息不应限于这些术语。这些术语仅用来将同一类型的信息彼此区分开，并不表示特定的顺序或者重要程度。实际上，“第一”、“第二”等表述完全可以互换使用。例如，在不脱离本公开范围的情况下，第一信息也可以被称为第二信息，类似地，第二信息也可以被称为第一信息。
图1至图16示出了根据本实施例的一种多通道LAMP检测仪，其能够基于LAMP反应自动进行核酸扩增检测。参照图1所示，该LAMP检测仪包括壳体1，壳体1内形成用于安装内部构件的内腔，壳体1上设有供微流控芯片100插入的芯片口101。壳体1内设有芯片仓2，芯片仓2具有多个用于容置微流控芯片100的芯片插槽10。具体到本实施例中，芯片仓2内具有三个芯片插槽10，每个芯片插槽10对应一个检测通道。在芯片仓2中，多个芯片插槽10沿前后方向并列设置，各芯片插槽10分别具有与芯片口101相对的槽口102，具体而言，芯片插槽10位于芯片口101的正下方，芯片插槽10的槽口面向上方， 微流控芯片100自上而下插入芯片插槽10中。
结合图1和图4所示，该多通道LAMP检测仪还包括可移动地设置于壳体1的内壁上的门3，门3用于将芯片口101封闭。具体地，门3可移动地设置于壳体1上侧部的内壁上。进一步地，壳体1上侧的内壁上设有沿左右方向延伸的第一导轨301，门3通过滑块可移动地设置于第一导轨301上。门3由第一驱动机构驱动开闭。具体地，第一驱动机构包括第一电机302及由第一电机302驱动转动的第一丝杆303，第一丝杆303和门3通过螺纹(例如通过丝杆螺母)连接。第一丝杆303能够由第一电机302驱动转动，通过丝杆和丝杆螺母的配合，将第一电机302的旋转运动转换为直线运动，从而能够让门3实现左右移动。第一导轨301有两个且互相平行，分别设置在门3的前后两侧。通过门传感器检测门3的开闭，门传感器和一门控制器电性连接，该门控制器还和第一电机302电性连接；当门传感器被触发后发出表示门3开启或关闭的检测信号，门控制器接收到该检测信号后向第一电机302发出控制其正转或反转的控制信号，从而驱动门3关闭或开启。该门控制器设置于一门控制PCB板93上，该PCB板93设于壳体1内壁上。在微流控芯片装入完成后，门3自动关闭将芯片口101封闭，以保护内部构件，较为美观。
参照图1、图2、图5及图6所示，该多通道LAMP检测仪还包括用于读取微流控芯片100上的条码的扫码器501。扫码器501通过扫码器支架502设于壳体1内，壳体1上设有扫描窗口503，扫码器501的扫描部位于扫描窗口503内或正对扫描窗口503。扫码器支架502包括基座5021及设于基座5021上的相对平行设置的支撑台5022，支撑台5022的形状为楔形。在基座5021上还设有连接孔13，扫码器支架502通过穿过连接孔13的连接件固定设置于壳体1的内壁上。芯片口101及扫码器501均位于壳体1的上侧部上且均朝向上方。
该多通道LAMP检测仪还包括主控制器及显示屏504(具体为触控显示屏)，主控制器分别和扫码器501及显示屏504电性连接。主控制器设置于主PCB板91上。显示屏504具有第一显示状态，在第一显示状态时，显示屏504具有扫码录入按钮。第一次按下扫码录入按钮后，扫码器501读取的微流控芯片100的信息和第一个检测通道(例如，最前侧的芯片插槽10)相关联；第二次按下扫码录入按钮后，扫码器501读取的微流控芯片100的信息和第二个检测通道(例如，中间的芯片插槽10)相关联；第三次按下扫码录入按钮后，扫码器501读取的微流控芯片100的信息和第三个检测通道(例如，最后侧的芯片插槽10)相关联。显示屏504位于壳体1的左侧部上。
参照图7至图8所示，该多通道LAMP检测仪还包括加热组件，加热组件用于对芯片插槽10内的微流控芯片100进行恒温加热。具体地说，加热组件用于对微流控芯片100的反应腔室进行加热。每个芯片插槽10分别对应至少一个加热组件，加热组件贴合设置于芯片插槽10的侧壁上。每个加热组件包括第一加热模块601和第二加热模块602，第一加热模块601包括第一保温棉压块6011、第一加热块6012、第一加热膜6013、第一保温棉6015和第一隔热片6014，第二加热模块602包括第二保温棉压块6021、第二加热块6022、第二加热膜6023、第二保温棉6025和第二隔热片6024。第一加热模块601和第二加热模块602的结构是类似的，区别在于，第二加热模块602的第二保温棉压块6021和第二加热块6022的形状与第一加热模块601的第一保温棉压块6011和第一加热块6012的形状不同。此处以第一加热模块601为例，第一加热块6012和第一隔热片6014覆盖设置于第一加热膜6013上，第一加热膜6013的表面具有胶层，第一加热膜6013贴合并覆盖设置于第一保温棉6015的正面上，第一保温棉6015的背面上设有第一保温棉压块6011，第一保温棉压块6011上具有连接孔13，通过穿过连接孔13的连接件将第一加热模块601设于芯片插槽10的侧壁上。第一加热块6012上固定设置有第一温度传感器603，第二加热块6022上固定设置有第二温度传感器604，第一温度传感器603、第二温度传感器604分别与一控制器(可以为上述主控制器)电性连接。该控制器用于根据第一温度传感器603检测到的温度信息相应控制供给第一加热膜6013的电压的大小，从而将第一加热块6012的加热温度维持恒定；相似地，该控制器用于根据第二温度传感器604检测到的温度信息相应控制供给第二加热膜6023的电压的大小，从而将第二加热块6022 的加热温度维持恒定。
图15a及图15b示出了芯片仓中单独一个芯片插槽10的局部结构，微流控芯片100可插设于芯片插槽10中。如图15a所示，芯片插槽10的侧壁上设有镂空区域10a，以便于与加热组件相配合；芯片插槽10的底壁上设有镂空区域10b，以便于与光学检测机构8相配合。第一加热块6012和芯片插槽10侧壁上部的一个镂空区域10a的形状相配合，并嵌入至该镂空区域10a中，从而能够和芯片插槽10中的微流控芯片贴合；类似地，第二加热块6022和芯片插槽10侧壁下部的一个镂空区域10a的形状相配合，并嵌入至该镂空区域10a中，从而能够和芯片插槽10中的微流控芯片贴合。此外，没有和微流控芯片贴合的部分设置有隔热片，即通过第一隔热片6014将第一加热模块601的高温部件和芯片插槽10的侧壁间隔开，通过第二隔热片6024将第二加热模块602的高温部件和芯片插槽10的侧壁间隔开，避免芯片插槽10长时间接触高热环境而导致的过早老化的问题。
参照图9及图10所示，多通道LAMP检测仪还包括机械臂机构7，机械臂机构7包括多个能够和芯片仓2中的微流控芯片100的活塞100a接合的机械臂，机械臂用于带动活塞100a移动。每个芯片插槽10对应至少一个机械臂，各机械臂能够沿左右方向移动地设置于壳体1内并位于芯片仓2的右侧。机械臂机构7包括设置于壳体1内壁的支架701、能够沿前后方向移动地设置于支架701上的上安装板702及能够沿左右方向设置于支架701上的下安装板703，上安装板702位于下安装板703的上方，上安装板702上设有多个第一机械臂704，下安装板703上设置有多个第二机械臂705，每个芯片插槽10分别与至少一个第一机械臂704及至少一个第二机械臂705对应，在初始位置时，第二机械臂705位于第一机械臂704的左下方。
参照图9、图10及图13所示，第一机械臂704和第二机械臂705分别具有可供微流控芯片100的活塞100a的端部(具体为活塞100a的颈部100a1)卡入的接合槽706，接合槽706具有面向上方的槽口102。在俯视时，能看到第一机械臂704和第二机械臂705前后且左右间隔设置，能够同时和两个活塞100a结合。具体而言，第二机械臂705位于第一机械臂704的下后方，即第二机械臂705位于第一机械臂704后侧一端距离，以和两个活塞100a的位置相配合。
参照图9及图10所示，机械臂机构7还包括用于驱动上安装板702左右移动的第二驱动机构、及用于驱动下安装板703左右移动的第三驱动机构，支架701上设置有沿左右方向延伸的第二导轨709和第三导轨(图中未示出)，上安装板702能够沿左右方向移动地设置于第二导轨709上，下安装板703能够沿左右方向移动地设置于第三导轨上，第二驱动机构包括第二电机7071及由第二电机7071驱动转动的第二丝杆7072，第二丝杠7072和上安装板702相连接，第三驱动机构包括第三电机7081及由第三电机7081驱动转动的第三丝杠(图中未示出)，第三丝杆和下安装板703相连接。机械臂机构7还包括用于检测第一机械臂704是否到达其初始位置的第一光电开关711、及用于检测第二机械臂705是否到达其初始位置的第二光电开关712。支架701具有用于阻止上安装板702和下安装板703向右移动至其设定行程之外的阻挡部7011。第二电机7071和第三电机7081还分别与机械臂控制器电性连接，机械臂控制器用于发出控制信号分别控制第二电机7071和第三电机7081的运行，机械臂控制器还分别与第一光电开关711和第二光电开关712电性连接，以接收来自第一光电开关711和第二光电开关712发出的信号。机械臂控制器设于机械臂控制PCB板92上。本实施例能够精确控制第一机械臂704和第二机械臂705，三个第一机械臂704由同一驱动系统控制，三个第二机械臂705由同一驱动系统控制，可以同步对三个微流控芯片100的活塞进行移动，控制精度较高，且避免过多增加成本。
机械臂控制器会发出控制信号至第二电机7071，使第二电机7071开始运行，第二电机7071驱动第二丝杆7072转动，带动上安装板702作直线运动。上安装板702上设置有第一机械臂704，第一机械臂704会跟着上安装板702一起作直线运动。第一机械臂704上设有第一光电开关感应支架7041，第一光电 开关感应支架7041会跟着第一机械臂704一起沿第二导轨709作直线运动，直至第一光电开关感应支架7041移到第一光电开关711处，第一光电开关711被触发而发出检测信号，并传输至机械臂控制器，机械臂控制器收到该检测信号后控制第二电机7071反向运行或停止。第二电机7071运行时，第二丝杆7072沿前后方向向前或向后移动，第一机械臂704随之沿第二导轨709向前或向后移动，从而带动微流控芯片100的上部的活塞100a左右移动，进而向微流控芯片100内的液体提供正压或负压，从而提供液体流通的动力。
类似地，机械臂控制器发出控制信号信号至第三电机7081，使第三电机7081开始运行，第三电机7081驱动第三丝杆转动，带动下安装板703作直线运动。下安装板703上设置有第二机械臂705，第二机械臂705会跟着下安装板703一起作直线运动。第二机械臂705上设有第二光电开关感应支架7051。第二光电开关感应支架7051会跟着第二机械臂705一起沿第三导轨作直线运动，直至第二光电开关感应支架7051移到第二光电开关712处，第二光电开关712被触发而发出检测信号，并传输机械臂控制器，机械臂控制器收到该检测信号后控制第三电机7081反向运行或停止运行。第三电机7081运行时，第三丝杆沿左右方向向左或向右移动，第二机械臂705随之沿第三导轨向左或向右移动，从而带动微流控芯片100的下部的活塞100a左右移动，进而向微流控芯片100内的液体提供正压或负压从而提供液体流通的动力，或切换微流控芯片100中腔室之间的通断。
参照图11a及图11b所示，该多通道LAMP检测仪包括光学检测机构8，光学检测机构8能够沿左右方向及前后方向移动地设置于壳体1内，光学检测机构8位于芯片仓2的下方，以对各个芯片插槽10中的微流控芯片100的各个扩增检测腔按照次序逐一检测，其中多个微流控芯片100沿前后方向排列，每个微流控芯片100具有沿左右方向间隔设置的多个扩增检测腔，每个扩增检测腔即为一个检测位点，如图16所示。例如，光学检测机构8沿左右方向移动，可以对某一个芯片插槽10内的微流控芯片100的多个检测位点进行逐一扫描；光学检测机构8沿前后方向移动一段距离，可以移动到和另一微流控芯片100对齐的位置上，然后再沿左右方向移动以对该微流控芯片100的多个检测位点进行逐一扫描。
进一步地，该多通道LAMP检测仪还包括设于壳体1内壁上的底座11，底座11具有沿左右方向延伸的X向导轨809；该多通道LAMP检测仪还包括能够沿左右方向移动地设于X向导轨809上的安装块，安装块具有沿前后方向延伸的Y向导轨810；光学检测机构8能够沿前后方向移动地设于Y向导轨810上。该多通道LAMP检测仪还包括用于驱动所述安装块沿X向导轨809移动的X向驱动机构、用于驱动光学检测机构8沿Y向导轨810移动的Y向驱动机构、用于检测光学检测机构8是否到达其在左右方向上的起始点的X向起点检测开关801、用于检测光学检测机构8是否到达其在左右方向上的终点的X向终点检测开关802、用于检测光学检测机构8是否到达其在前后方向上的起始点的Y向起点检测开关803、及用于检测光学检测机构8是否到达其在前后方向上的终点的Y向终点检测开关804，X向起点检测开关801和X向终点检测开关802分别靠近X向导轨809的两端部，Y向起点检测开关803和Y向终点检测开关804分别靠近Y向导轨810的两端部。具体到本实施例中，X向驱动机构包括X向电机805，X向电机805设置于底座11上并通过丝杆和螺母配合驱动Y向导轨810左右移动；Y向驱动机构包括Y向电机806，Y向电机806设置于安装块上并通过丝杆和螺母配合驱动光学检测机构8沿Y向导轨前后移动。X向起点检测开关801、X向终点检测开关802、Y向起点检测开关803、Y向终点检测开关804分别为光电检测开关。参照图16所示，X向起点检测开关801在检测到光学检测机构8到达其在左右方向上的起点时发出第一检测信号，X向终点检测开关802用于在检测到光学检测机构8到达其在左右方向上的终点时发出第二检测信号，Y向起点检测开关803用于在检测到光学检测机构8到达其在前后方向上的起点时发出第三检测信号，Y向终点检测开关804用于在检测到光学检测机构8到达其在前后方向上的终点时发出第四检测信号。
光学检测机构8具有多个检测位置，各芯片插槽10分别具有沿左右方向排列的多个检测位点，每个 芯片插槽10的每个检测位点分别对应一个检测位置。具体到本实施例中，如图16所示，每个芯片插槽10中的微流控芯片对应一个检测通道，共三个检测通道C1、C2和C3，每个芯片插槽10分别具有四个检测位点，共有三个芯片插槽10，故具有十二个检测位点；具体而言，检测通道C1具有四个检测位点P11、P12、P13和P14，检测通道C2具有四个检测位点P21、P22、P23和P24；检测通道C3具有四个检测位点P31、P32、P33和P34。以位于最前方的芯片插槽10为例，使光学检测机构8从原点开始运行，按照检测位点所组成的坐标进行运动，光学检测机构8经过四个检测位点之后，使光学检测机构8沿Y方向移动至后一个芯片插槽10，对第二个芯片插槽10进行检测，以此类推。
主控制器分别和X向起点检测开关801、X向终点检测开关802、Y向起点检测开关803、Y向终点检测开关804电性连接，在接收到第一检测信号后控制X向驱动机构正向运行，及在接收到第二检测信号后控制X向驱动机构反向运行，及在接收到第三检测信号后控制所述Y向驱动机构正向运行，及在接收到第四检测信号后控制所述Y向驱动机构反向运行。
参照图3及图12所示，壳体1内设有安装架101，安装架101位于显示屏504和芯片仓2之间。安装架101上设有导向滑槽101a，主PCB板91的边缘部位插设于导向滑槽101a中。能够用简单的结构及成本低的方式安装PCB板，体积小巧，且安装方便，尤其是在安装空间比较狭窄的情况下，装配和维护均较为方便。
如图14所示，该多通道LAMP检测仪还包括芯片罩4，芯片罩4设于围绕芯片口101的壳体1内壁和芯片仓2之间以及芯片插槽10之间，其镂空部分和各芯片插槽10的槽口对应以允许微流控芯片100插入到芯片插槽10中。芯片罩4用于将芯片仓2和壳体1内壁之间的缝隙及芯片插槽10之间的缝隙封闭，防止异物掉入，防尘，保护内部构件。芯片罩4还能对检测仪内部的零部件起一个遮挡的作用，较为美观。芯片罩4采用3D打印技术，结构简单的同时，能够解决其制造成本贵的问题。
本实施例的多通道LAMP检测仪的工作过程如下：
将微流控芯片100对准扫码器501扫码后，将其插入芯片插槽10中，门3自动关闭，机械臂机构7的第一机械臂704和第二机械臂705按照设定时序移动，带动活塞100a移动，连通腔体，并提供流体流动的正压或负压，以使微流控芯片100的样本、试剂混合或使试剂流通至目标腔室中，直至进入反应腔中进行扩增反应；对加热组件上电，使其对微流控芯片100的相应腔室加热；反应结束后，光学检测机构8上电，并按照设定时序依次移动至各个检测位点，对每个扩增检测腔进行光学检测。
本实施例还提供一种多通道LAMP检测仪的控制方法，其包括如下过程：
A、将微流控芯片100扫码录入样本信息等并和对应的检测通道C1、C2或C3关联，即安装微流控芯片100；
B、对微流控芯片100进行恒温加热：
C、控制第一机械臂704和第二机械臂705移动，使微流控芯片100的样本、引物等反应体系混合及分配至各扩增腔中；
D、控制光学检测机构8移动逐个对检测位点检测。
参照图16所示，过程D具体包括如下步骤：
S101、光学检测机构8处于原点位置或将其移动至原点位置(即检测位点P11)，X向起点检测开关801和Y向起点检测开关803均被触发而发出第一检测信号和第三检测信号，主控制器接收到第一检测信 号和第三检测信号后，判断光学检测机构8处于原点，并对第一个微流控芯片100的检测位点P11进行检测。主控制器将光学检测机构8此时发送的检测信息与第一个检测通道C1的检测位点P11建立对应关系。
S102、主控制器控制X向电机805正向运行，使光学检测机构8向右移动设定距离d1后停止到检测位点P12以进行检测；具体为，通过设定距离d1(即相邻两个检测位点的间距)换算出X向电机805的转动角度，当X向电机805转过设定角度后即停止。主控制器将光学检测机构8此时发送的检测信息与第一个检测通道C1的检测位点P12建立对应关系。
以此类推，控制光学检测机构8依次移动至检测位点P13、P14处进行检测，主控制器依次将光学检测机构8发送的检测信息与第一个检测通道C1的检测位点P13、P14建立对应关系。
在对第一个微流控芯片100的检测位点P14检测时，X向终点检测开关802被触发而发出第二检测信号。
S103、主控制器接收到第二检测信号且等待设定时间后，控制X向电机805反向运行，使光学检测机构8向左移动至回到原点，此时X向起点检测开关801被触发而发出第一检测信号；该设定时间至少大于光学检测机构8完成对一个检测位点的光学检测所需的时间。
S104、光学检测机构8返回原点后，主控制器接收到第一检测信号和第三检测信号，控制Y向电机806向后移动设定距离d2后停止到第二个微流控芯片100的检测位点P21以进行检测；主控制器将光学检测机构8此时发送的检测信息与第二个检测通道C2的检测位点P21建立对应关系。具体为，通过设定距离d2(即相邻两个微流控芯片100的间距)换算出Y向电机806的转动角度，当Y向电机806转过设定角度后即停止
S105、主控制器控制X向电机805正向运行，使光学检测机构8向右移动设定距离d1后停止到检测位点P22以进行检测；主控制器将光学检测机构8此时发送的检测信息与第二个检测通道C2的检测位点P22建立对应关系。
以此类推，控制光学检测机构8依次移动至检测位点P23、P24处进行检测，主控制器依次将光学检测机构8发送的检测信息与第二个检测通道C2的检测位点P23、P24建立对应关系。
在对第二个微流控芯片100的检测位点P24检测时，X向终点检测开关802被触发而发出第二检测信号。
S106、主控制器接收到第二检测信号且等待所述设定时间后，控制X向电机805反向运行，使光学检测机构8向左移动，直至X向起点检测开关801被触发而发出第一检测信号。
S107、光学检测机构8返回原点后，主控制器接收到第一检测信号，主控制器接收到第一检测信号后，控制Y向电机806向后移动d2至最后一个微流控芯片100的检测位点P31以进行检测；主控制器将光学检测机构8此时发送的检测信息与第三个检测通道C3的检测位点P31建立对应关系。
S108、主控制器控制X向电机805正向运行，使光学检测机构8向右移动设定距离d1后停止到检测位点P32以进行检测；主控制器将光学检测机构8此时发送的检测信息与第三个检测通道C3的检测位点P32建立对应关系。
以此类推，控制光学检测机构8依次移动至检测位点P33、P34处进行检测，主控制器依次将光学检测机构8发送的检测信息与第三个检测通道C3的检测位点P33、P34建立对应关系。
在对第三个微流控芯片100的检测位点P44检测时，X向终点检测开关802被触发而发出第二检测信号。
S109、主控制器接收到第二检测信号且等待所述设定时间后后，控制X向电机805反向运行，使光学检测机构8向左移动，直至X向起点检测开关801被触发而发出第一检测信号；同时，在对第三个检测通道进行检测的过程中，Y向终点检测开关804被触发而发出第四检测信号；
S110、主控制器接收到第一检测信号和第四检测信号后，控制Y向电机806反向运行，使光学检测机构8向前移动直至X向起点检测开关801和Y向起点检测开关803均被触发而发出第一检测信号和第三检测信号，Y向电机806停止运行，使光学检测机构8返回至原点，等待下一次检测。
过程A具体包括：
S201、将显示屏504切换为第一显示状态，通过显示屏504接收用户的第一次扫码输入指令，通过扫码器501读取第一个微流控芯片100的第一条码信息，主控制器在接收到所述第一次扫码输入指令和所述第一条码信息后，将所述第一条码信息和第一个检测通道C1相关联；
S202、通过显示屏504接收用户的第二次扫码输入指令，通过扫码器501读取第二个微流控芯片100的第二条码信息，主控制器在接收到所述第二次扫码输入指令和所述第二条码信息后，将所述第二条码信息和第二个检测通道C2相关联；
S203、通过显示屏504接收用户的第三次扫码输入指令，通过扫码器501读取第三个微流控芯片100的第三条码信息，主控制器在接收到所述第三次扫码输入指令和所述第二条码信息后，将所述第三条码信息和第三个检测通道C3相关联。
上述的条码信息包括待测样本信息，如编号、对应的受检者姓名、来源等。
检测完成后，将显示屏504切换为第二显示状态，将各个检测位点的检测结果(阴/阳)及关联的编号、受检者姓名等进行显示。
本实施例提供的一种多通道LAMP检测仪，芯片仓2内设有多个芯片插槽10，能够同时对多个芯片进行检测，能够一次对多个微流控芯片100进行处理，检测程序运行一次所能处理的样本数量大大提高，具有较高的检测效率；设置的门3及芯片罩4能够防止灰尘及异物进入芯片仓2和内壁之间的缝隙中，其中，芯片罩4还能对检测仪内部的元件起遮挡作用，使外观更加美观；该LAMP检测仪的多个检测通道可以共用一些部件，例如机械臂机构、光学检测机构8等，相比单通道的LAMP检测仪，有效利用了空间，整机体积并不会明显增加，不会额外占用空间。在增设了多个检测通道的前提下，该检测仪的体积依然与单通道的检测仪相仿，结构更加紧凑，优化占用空间，体积小巧，操作简单，使用方便，节约成本。
上述实施例只为说明本发明的技术构思及特点，是一种优选的实施例，其目的在于熟悉此项技术的人士能够了解本发明的内容并据以实施，并不能以此限定本发明的保护范围。凡根据本发明的原理所作的等效变换或修饰，都应涵盖在本发明的保护范围之内。

Claims (15)

  1. 一种多通道LAMP检测仪，包括芯片仓和光学检测机构，其特征在于，所述芯片仓具有多个用于容置微流控芯片的芯片插槽，多个所述芯片插槽沿前后方向并列设置；所述光学检测机构能够沿左右方向及前后方向移动地设置于所述壳体内，所述光学检测机构位于所述芯片仓的下方；各所述芯片插槽分别具有沿左右方向排列的多个检测位点，所述光学检测机构具有多个检测位置，每个所述芯片插槽的每个所述检测位点分别对应一个所述检测位置；所述多通道LAMP检测仪还包括：
    X向驱动机构，其用于驱动所述光学检测机构沿左右方向移动；
    Y向驱动机构，其用于驱动所述光学检测机构沿前后方向移动；
    X向起点检测开关，其用于在检测到所述光学检测机构到达其在左右方向上的起点时发出第一检测信号；
    X向终点检测开关，其用于在检测到所述光学检测机构到达其在左右方向上的终点时发出第二检测信号；
    Y向起点检测开关，其用于在检测到所述光学检测机构到达其在前后方向上的起点的第三检测信号；
    Y向终点检测开关，其用于在检测所述光学检测机构到达其在前后方向上的终点的第四检测信号；
    控制器，其用于接收所述X向起点检测开关、X向终点检测开关、Y向起点检测开关及Y向终点检测开关的检测信号，并能够在接收到所述第一检测信号后控制所述X向驱动机构正向运行，及能够在接收到所述第二检测信号后控制所述X向驱动机构反向运行，及能够在接收到所述第三检测信号后控制所述Y向驱动机构正向运行，及能够在接收到所述第四检测信号后控制所述Y向驱动机构反向运行。
  2. 根据权利要求1所述的多通道LAMP检测仪，其特征在于，
    所述控制器，用于在接收到所述第一检测信号和所述第三检测信号时，控制所述X向驱动驱动机构正向运行第一设定距离后停止设定时间，重复该过程一或多次，直至接收到所述第二检测信号后，控制所述X向驱动机构反向运行；直至再次接收到所述第一检测信号后，控制所述X向驱动机构停止运行。
  3. 根据权利要求1所述的多通道LAMP检测仪，其特征在于，
    所述控制器，还用于在接收到所述第一检测信号和所述第三检测信号时控制所述Y向驱动机构正向运行第二设定距离后停止设定时间；控制所述X向驱动驱动机构正向运行第一设定距离后停止设定时间，重复该过程一或多次，直至接收到所述第二检测信号后，控制所述X向驱动机构反向运行；直至再次接收到所述第一检测信号后，控制所述X向驱动机构停止运行。
  4. 根据权利要求1所述的多通道LAMP检测仪，其特征在于，
    所述控制器，还用于在接收到所述第一检测信号时控制所述Y向驱动机构正向运行，直至接收到所述第四检测信号，控制所述Y向驱动机构停止运行；控制所述X向驱动驱动机构正向运行第一设定距离后停止设定时间，重复该过程一或多次，直至接收到所述第二检测信号后，控制所述X向驱动机构反向运行；直至再次接收到所述第一检测信号后，控制所述X向驱动机构停止运行。
  5. 根据权利要求1所述的多通道LAMP检测仪，其特征在于，
    所述控制器，还用于在接收到所述第四检测信号和所述第一检测信号时控制所述Y向驱动机构反向运行，直至接收到所述第三检测信号，控制所述Y向驱动机构停止运行。
  6. 根据权利要求1至5任一项所述的多通道LAMP检测仪，其特征在于，所述X向驱动机构包括X向电机，所述Y向驱动机构包括Y向电机，所述控制器分别和所述X向电机及所述Y向电机电性连接。
  7. 根据权利要求6所述的多通道LAMP检测仪，其特征在于，所述多通道LAMP检测仪包括底座，所述底座具有多个并列设置且沿左右方向延伸的X向导轨；所述多通道LAMP检测仪还包括能够沿左右方向移动地设于所述X向导轨上的安装块，所述安装块上设有沿前后方向延伸的Y向导轨；所述光学检测机构能够沿前后方向移动地设于所述Y向导轨上；所述X向电机设于所述底座上并通过X向丝杆连接于所述安装块或所述Y向导轨，所述Y向电机设于所述安装块上并通过Y向丝杆连接于所述光学检测机构；所述X向起点检测开关和所述X向终点检测开关分别靠近所述X向导轨的两端部，所述Y向起点检测开关和所述Y向终点检测开关分别靠近所述Y向导轨的两端部。
  8. 根据权利要求1至5任一项所述的多通道LAMP检测仪，其特征在于，所述控制器分别和所述X向起点检测开关、X向终点检测开关、Y向起点检测开关及Y向终点检测开关电性连接
  9. 根据权利要求1所述的多通道LAMP检测仪，其特征在于，所述多通道LAMP检测仪还包括用于读取微流控芯片上的条码的扫码器、及用作人机交互界面的显示屏，所述显示屏具有第一显示状态，在所述第一显示状态时，所述显示屏具有用于供用户输入扫码录入指令的扫码录入按钮，所述控制器还用于在接收到所述扫码录入指令后按照设定次序将所述扫码器读取的条码信息和对应的检测通道建立对应关系，每个检测通道对应一个所述芯片插槽。
  10. 根据权利要求9所述的多通道LAMP检测仪，其特征在于，所述控制器还用于接收所述光学检测机构返回的检测数据，所述显示屏还具有第二显示状态，在所述第二显示状态时，所述显示屏具有对应各个检测通道的各个检测位点的结果显示区域。
  11. 根据权利要求1所述的多通道LAMP检测仪，其特征在于，所述多通道LAMP检测仪还包括壳体，所述壳体上设有供微流控芯片插入的芯片口，各所述芯片插槽分别具有与所述芯片口相对的槽口；所述多通道LAMP检测仪还包括用于对所述芯片槽内的微流控芯片进行恒温加热的加热组件，每个所述芯片插槽的侧壁上分别设有至少一个所述加热组件；所述多通道LAMP检测仪还包括机械臂机构，所述机械臂机构包括多个能够和所述芯片仓中的微流控芯片的活塞接合的机械臂，每个所述芯片插槽对应至少一个所述机械臂，各所述机械臂能够沿左右方向移动地设置于所述壳体内并位于所述芯片仓的右侧。
  12. 根据权利要求11所述的多通道LAMP检测仪，其特征在于，所述机械臂机构包括设置于所述壳体内壁的支架、能够沿左右方向设置于所述支架上的下安装板及能够沿左右方向移动地设置于所述支架上的上安装板，所述上安装板位于所述下安装板的上方，所述上安装板上设有多个第一机械臂，所述下安装板上设置有多个第二机械臂，每个所述芯片槽分别与至少一个所述第一机械臂及至少一个所述第二机械臂对应，在初始位置时，所述第二机械臂位于所述第一机械臂的左下方。
  13. 一种如权利要求1至12任一项所述的多通道LAMP检测仪的控制方法，其特征在于，所述控制方法包括如下步骤：
    S101、控制器接收到第一检测信号和第三检测信号后，判断所述光学检测机构处于原点，并对第一个微流控芯片的检测位点P11进行检测；
    S102、控制器控制所述X向驱动机构正向运行，使所述光学检测机构向右移动设定距离d1后停止到检测位点P12以进行检测；以此类推，直至完成对第一个微流控芯片的所有检测位点的检测，此时所述X向终点检测开关被触发而发出第二检测信号；
    S103、控制器接收到所述第二检测信号后，控制所述X向驱动机构反向运行，使所述光学检测机构向左移动至回到原点，此时所述X向起点检测开关被触发而发出第一检测信号；
    S104、控制器接收到所述第一检测信号和所述第三检测信号后，控制所述Y向驱动机构向后移动设定距离d2后停止到第二个微流控芯片的检测位点P21以进行检测；
    S105、控制器控制所述X向驱动机构正向运行，使所述光学检测机构向右移动设定距离d1后停止到检测位点P22以进行检测；以此类推，直至完成对第二个微流控芯片的所有检测位点的检测，此时所述X向终点检测开关被触发而发出第二检测信号；
    S106、控制器接收到所述第二检测信号后，控制所述X向驱动机构反向运行，使所述光学检测机构向左移动，直至所述X向起点检测开关被触发而发出第一检测信号；
    S107、控制器接收到所述第一检测信号后，控制所述Y向驱动机构向后移动至最后一个微流控芯片的检测位点Pn1以进行检测，n为检测通道的个数；
    S108、控制器控制所述X向驱动机构正向运行，使所述光学检测机构向右移动设定距离d1后停止到检测位点Pn2以进行检测；以此类推，直至完成对最后一个微流控芯片的所有检测位点的检测，此时所述X向终点检测开关被触发而发出第二检测信号；
    S109、控制器接收到所述第二检测信号后，控制所述X向驱动机构反向运行，使所述光学检测机构向左移动，直至所述X向起点检测开关被触发而发出第一检测信号，此时所述Y向终点检测开关被触发而发出第四检测信号；
    S110、控制器接收到所述第一检测信号和所述第四检测信号后，控制所述Y向驱动机构反向运行，使所述光学检测机构向前移动直至所述X向起点检测开关和所述Y向起点检测开关均被触发而发出第一检测信号和第三检测信号，所述Y向驱动机构停止运行。
  14. 根据权利要求13所述的控制方法，其特征在于，所述控制方法还包括如下步骤：
    S201、通过显示屏接收用户的第一次扫码输入指令，通过扫码器读取第一个微流控芯片的第一条码信息，控制器在接收到所述第一次扫码输入指令和所述第一条码信息后，将所述第一条码信息和第一个检测通道相关联；
    S202、通过所述显示屏接收用户的第二次扫码输入指令，通过所述扫码器读取第二个微流控芯片的第二条码信息，所述控制器在接收到所述第二次扫码输入指令和所述第二条码信息后，将所述第二条码信息和第二个检测通道相关联；
    以此类推，直至将所有的检测通道和微流控芯片的条码信息对应关联。
  15. 根据权利要求14所述的控制方法，其特征在于，
    所述步骤S101中，所述控制器将所述光学检测机构发送的检测信息与第一个检测通道的检测位点P11建立对应关系；
    所述步骤S102中，所述控制器将所述光学检测机构发送的检测信息与第一个检测通道的其他检测位点依次建立对应关系；
    所述步骤S104中，所述控制器将所述光学检测机构发送的检测信息与第二个检测通道的检测位点P21建立对应关系；
    所述步骤S105中，所述控制器将所述光学检测机构发送的检测信息与第二个检测通道的其他检测位点依次建立对应关系；
    所述步骤S107中，所述控制器将所述光学检测机构发送的检测信息与最后一个检测通道的检测位点Pn1建立对应关系；
    所述步骤S108中，所述控制器将所述光学检测机构发送的检测信息与最后一个检测通道的其他检测位点依次建立对应关系。
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