WO2023048581A1 - Protection de plantes contre les dommages causés par le gel - Google Patents
Protection de plantes contre les dommages causés par le gel Download PDFInfo
- Publication number
- WO2023048581A1 WO2023048581A1 PCT/NZ2022/050118 NZ2022050118W WO2023048581A1 WO 2023048581 A1 WO2023048581 A1 WO 2023048581A1 NZ 2022050118 W NZ2022050118 W NZ 2022050118W WO 2023048581 A1 WO2023048581 A1 WO 2023048581A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- acid
- silicate
- fatty acid
- plant
- bacteria
- Prior art date
Links
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 48
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 47
- 239000000194 fatty acid Substances 0.000 claims abstract description 47
- 229930195729 fatty acid Natural products 0.000 claims abstract description 47
- 241000894006 Bacteria Species 0.000 claims abstract description 37
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 33
- 230000006910 ice nucleation Effects 0.000 claims abstract description 27
- 239000000725 suspension Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 42
- 241000196324 Embryophyta Species 0.000 claims description 40
- 239000000203 mixture Substances 0.000 claims description 19
- 241000589615 Pseudomonas syringae Species 0.000 claims description 13
- 235000013399 edible fruits Nutrition 0.000 claims description 13
- 239000000344 soap Substances 0.000 claims description 13
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 claims description 12
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 claims description 10
- UKMSUNONTOPOIO-UHFFFAOYSA-N docosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCCCC(O)=O UKMSUNONTOPOIO-UHFFFAOYSA-N 0.000 claims description 10
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 claims description 10
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 claims description 10
- VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 claims description 10
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 10
- 235000019353 potassium silicate Nutrition 0.000 claims description 10
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 8
- 240000008574 Capsicum frutescens Species 0.000 claims description 8
- TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 claims description 8
- 239000004111 Potassium silicate Substances 0.000 claims description 8
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims description 8
- 235000013339 cereals Nutrition 0.000 claims description 8
- SECPZKHBENQXJG-FPLPWBNLSA-N palmitoleic acid Chemical compound CCCCCC\C=C/CCCCCCCC(O)=O SECPZKHBENQXJG-FPLPWBNLSA-N 0.000 claims description 8
- 229910052913 potassium silicate Inorganic materials 0.000 claims description 8
- NNHHDJVEYQHLHG-UHFFFAOYSA-N potassium silicate Chemical compound [K+].[K+].[O-][Si]([O-])=O NNHHDJVEYQHLHG-UHFFFAOYSA-N 0.000 claims description 8
- 235000013311 vegetables Nutrition 0.000 claims description 8
- 229960004232 linoleic acid Drugs 0.000 claims description 7
- 239000004115 Sodium Silicate Substances 0.000 claims description 6
- PAZHGORSDKKUPI-UHFFFAOYSA-N lithium metasilicate Chemical compound [Li+].[Li+].[O-][Si]([O-])=O PAZHGORSDKKUPI-UHFFFAOYSA-N 0.000 claims description 6
- 229910052912 lithium silicate Inorganic materials 0.000 claims description 6
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 6
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 6
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 6
- 159000000000 sodium salts Chemical class 0.000 claims description 5
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 4
- TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 claims description 4
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 4
- 241000208140 Acer Species 0.000 claims description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 4
- 240000006108 Allium ampeloprasum Species 0.000 claims description 4
- 235000005254 Allium ampeloprasum Nutrition 0.000 claims description 4
- 244000291564 Allium cepa Species 0.000 claims description 4
- 235000002732 Allium cepa var. cepa Nutrition 0.000 claims description 4
- 235000007319 Avena orientalis Nutrition 0.000 claims description 4
- 244000075850 Avena orientalis Species 0.000 claims description 4
- 235000021357 Behenic acid Nutrition 0.000 claims description 4
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 4
- 241000335053 Beta vulgaris Species 0.000 claims description 4
- 241000167854 Bourreria succulenta Species 0.000 claims description 4
- 235000011331 Brassica Nutrition 0.000 claims description 4
- 241000219198 Brassica Species 0.000 claims description 4
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 claims description 4
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 claims description 4
- 235000002566 Capsicum Nutrition 0.000 claims description 4
- 240000006766 Cornus mas Species 0.000 claims description 4
- 244000193629 Cyphomandra crassifolia Species 0.000 claims description 4
- 235000000298 Cyphomandra crassifolia Nutrition 0.000 claims description 4
- 244000000626 Daucus carota Species 0.000 claims description 4
- 235000002767 Daucus carota Nutrition 0.000 claims description 4
- 240000005979 Hordeum vulgare Species 0.000 claims description 4
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 4
- 244000017020 Ipomoea batatas Species 0.000 claims description 4
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 4
- 240000008415 Lactuca sativa Species 0.000 claims description 4
- 235000003228 Lactuca sativa Nutrition 0.000 claims description 4
- 239000005639 Lauric acid Substances 0.000 claims description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 4
- 241000218378 Magnolia Species 0.000 claims description 4
- 244000070406 Malus silvestris Species 0.000 claims description 4
- 235000021360 Myristic acid Nutrition 0.000 claims description 4
- 239000005642 Oleic acid Substances 0.000 claims description 4
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 235000021314 Palmitic acid Nutrition 0.000 claims description 4
- 235000021319 Palmitoleic acid Nutrition 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 240000004713 Pisum sativum Species 0.000 claims description 4
- 235000010582 Pisum sativum Nutrition 0.000 claims description 4
- 241000219000 Populus Species 0.000 claims description 4
- 244000018633 Prunus armeniaca Species 0.000 claims description 4
- 235000009827 Prunus armeniaca Nutrition 0.000 claims description 4
- 240000005809 Prunus persica Species 0.000 claims description 4
- 235000006029 Prunus persica var nucipersica Nutrition 0.000 claims description 4
- 235000006040 Prunus persica var persica Nutrition 0.000 claims description 4
- 244000017714 Prunus persica var. nucipersica Species 0.000 claims description 4
- 241000589516 Pseudomonas Species 0.000 claims description 4
- 241000220324 Pyrus Species 0.000 claims description 4
- 241000220317 Rosa Species 0.000 claims description 4
- 240000003768 Solanum lycopersicum Species 0.000 claims description 4
- 244000061456 Solanum tuberosum Species 0.000 claims description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 4
- 240000003829 Sorghum propinquum Species 0.000 claims description 4
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 4
- 235000021355 Stearic acid Nutrition 0.000 claims description 4
- 244000297179 Syringa vulgaris Species 0.000 claims description 4
- 235000004338 Syringa vulgaris Nutrition 0.000 claims description 4
- 235000021307 Triticum Nutrition 0.000 claims description 4
- 244000098338 Triticum aestivum Species 0.000 claims description 4
- 241000219094 Vitaceae Species 0.000 claims description 4
- 241000589634 Xanthomonas Species 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims description 4
- 235000021016 apples Nutrition 0.000 claims description 4
- 229940116226 behenic acid Drugs 0.000 claims description 4
- 235000021028 berry Nutrition 0.000 claims description 4
- 239000001390 capsicum minimum Substances 0.000 claims description 4
- 235000019693 cherries Nutrition 0.000 claims description 4
- SECPZKHBENQXJG-UHFFFAOYSA-N cis-palmitoleic acid Natural products CCCCCCC=CCCCCCCCC(O)=O SECPZKHBENQXJG-UHFFFAOYSA-N 0.000 claims description 4
- 239000003925 fat Substances 0.000 claims description 4
- 235000019197 fats Nutrition 0.000 claims description 4
- LQJBNNIYVWPHFW-QXMHVHEDSA-N gadoleic acid Chemical compound CCCCCCCCCC\C=C/CCCCCCCC(O)=O LQJBNNIYVWPHFW-QXMHVHEDSA-N 0.000 claims description 4
- 235000021021 grapes Nutrition 0.000 claims description 4
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 4
- 235000020778 linoleic acid Nutrition 0.000 claims description 4
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 claims description 4
- 229960004488 linolenic acid Drugs 0.000 claims description 4
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 claims description 4
- 229910003002 lithium salt Inorganic materials 0.000 claims description 4
- 159000000002 lithium salts Chemical class 0.000 claims description 4
- 235000009973 maize Nutrition 0.000 claims description 4
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 claims description 4
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 4
- 229960002446 octanoic acid Drugs 0.000 claims description 4
- 239000003921 oil Substances 0.000 claims description 4
- 235000021017 pears Nutrition 0.000 claims description 4
- 235000021018 plums Nutrition 0.000 claims description 4
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 239000008117 stearic acid Substances 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 20
- 239000012085 test solution Substances 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 9
- 150000004760 silicates Chemical class 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 229910052782 aluminium Inorganic materials 0.000 description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 229910021645 metal ion Inorganic materials 0.000 description 5
- 239000012188 paraffin wax Substances 0.000 description 5
- 239000013526 supercooled liquid Substances 0.000 description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 239000002667 nucleating agent Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 240000001140 Mimosa pudica Species 0.000 description 2
- 235000016462 Mimosa pudica Nutrition 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 108010077805 Bacterial Proteins Proteins 0.000 description 1
- 241000520272 Pantoea Species 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000589623 Pseudomonas syringae pv. syringae Species 0.000 description 1
- 241000589636 Xanthomonas campestris Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000006911 nucleation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000003094 perturbing effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/06—Devices for generating heat, smoke or fog in gardens, orchards or forests, e.g. to prevent damage by frost
- A01G13/065—Frost protection by generating fog or by spraying
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
Definitions
- This invention relates to a method and composition for protecting plants from frost damage caused by ice nucleating agents.
- frost damage at temperatures of around 0° C.
- the damage tends to be mechanical in nature, caused by the formation of ice within the plant tissue. Initially the ice may form on the plant surface or in its intercellular spaces, and then propagate through cell membranes, rupturing them.
- a number of species of bacteria can act as ice nucleating agents and these may occur as epiphytes on plants. Examples include Pseudomonas syringae pv Syringae, certain strains of Pseudomonas fluorescence, certain pathovars of Xanthomonas campestris and various strains of Pantoea. Such bacteria are able to catalyse ice formation at temperatures as warm as about -2° C.
- the ice forming temperature for frost sensitive plants is dependent on the population size of the ice nucleating bacteria on them. Larger bacterial populations tend to be associated with ice nucleation at warmer temperatures. However, in the absence of such organisms the water in some plants is able to supercool to about -6° C. Therefore, the adverse impact of ice nucleating bacteria is significant.
- the current invention is thought to be effective in not only killing the bacteria but also in substantially denaturing or disassociating proteins that form part of the bacteria so that they have no, or less, ability to cause ice nucleation.
- the invention is a method of protecting a plant from frost damage, comprising applying to the exterior of the plant:
- silicate in solution or suspension in an amount sufficient to protectively interfere with bacteria induced ice nucleation.
- the fatty acid and/or the silicate are in solution or suspension in water.
- the bacteria is gram negative.
- the bacteria comprise Pseudomonas (eg Pseudomonas syringae pv Syringae), Xanthomonas and/or Panatoa bacteria.
- Pseudomonas eg Pseudomonas syringae pv Syringae
- Xanthomonas e.g. Xanthomonas and/or Panatoa bacteria.
- the fatty acid and silicate dissociate or denature ice nucleation inducing protein.
- the fatty acid and silicate are applied to the plant in combination.
- the fatty acid is in soap form.
- the fatty acid is in the form of one or more of:
- the fatty acid is derived from fats of animal origin.
- the fatty acid is derived from oils of plant origin.
- the fatty acid is derived from fats or oils of plant or animal origin.
- the fatty acid comprises one or more of -
- Linoleic Acid eg alpha Linoleic Acid
- fatty acid comprises one or more of -
- the silicate is water soluble.
- the silicate is in the form of a metallic salt.
- the silicate comprises one or more of:
- the molar ratio of the silicate ranges from 2.0 to 3.3.
- the plant is one or more of a fruit, vegetable, flower, grain, mushroom or tree.
- the fruit is one or more of apples, pears, peaches, nectarines, apricots, plums, cherries, tamarillos, grapes and berry fruit.
- the vegetable comprises one or more of lettuce, brassicas, cucurbits, tomato, capsicum, chilli, potato, sweet potato, carrots, beet, spring onions, leeks, beans and peas.
- the grain comprises one or more of wheat, maize, sorghum, oats, rice and barley.
- the tree comprises an ornamental variety selected from one or more, of magnolia, poplar, dogwood, maple, lilac and rose.
- the bacteria comprise Pseudomonas syringae pv. Syringae.
- the invention is the use of:
- silicate in the production of a composition for protecting a plant from frost damage by spraying the surface of the plant with the composition in an amount sufficient to protectively interfere with bacteria induced ice nucleation.
- the fatty acid and silicate are in solution or suspension in water.
- the bacteria comprise Pseudomonas (eg Pseudomonas syringae pv Syringae), Xanthomonas and/or Panatoa bacteria.
- Pseudomonas eg Pseudomonas syringae pv Syringae
- Xanthomonas e.g. Xanthomonas and/or Panatoa bacteria.
- the fatty acid is in soap form.
- the fatty acid is in the form of one or more of:
- the silicate is in the form of a metallic salt.
- the silicate comprises one or more of:
- the fatty acid comprises one or more of the fatty acid substances listed above.
- the plant is one or more of a fruit, vegetable, flower, grain, mushroom or tree.
- the fruit is one or more of apples, pears, peaches, nectarines, apricots, plums, cherries, tamarillos, grapes and berry fruit.
- the vegetable comprises one or more of lettuce, brassicas, cucurbits, tomato, capsicum, chilli, potato, sweet potato, carrots, beet, spring onions, leeks, beans and peas.
- the grain comprises one or more of wheat, maize, sorghum, oats, rice and barley.
- the tree comprises an ornamental variety selected from one or more, of magnolia, poplar, dogwood, maple, lilac and rose.
- the invention is a composition comprising:
- silicate for protecting a plant from frost damage by spraying the surface of the plant with the composition in an amount sufficient to protectively interfere with bacteria induced ice nucleation.
- the fatty acid, silicate and bacteria may be as per any one or more of the statements above.
- the invention relates to a method of protecting plants from frost damage resulting from the presence of ice nucleating bacteria on the plant surfaces. This is of particular benefit to plant foliage, fruit or flowers (which should all be considered to be part of the plant).
- the protection is achieved by spraying a composition comprising one or more suitable fatty acids and silicates mixed in a watery carrier onto the exterior of the plant.
- Isolate 31 R1 is regarded as the most active strain of bacteria in terms of causing ice nucleation.
- Test Solutions were prepared using HML Silco (also called PS1) and NSA, which are composed as follows -
- Test Solutions were used as or made up into Test Samples as follows -
- the Bacterial Suspension was added to each Test Sample 30 minutes after it had been formed, to a concentration of 10 7 cells per mL. Then, after 15 minutes, the 40 x 10 pL droplets of each Test Sample (which by then incorporated the Bacterial Suspension) were placed on the surface of a paraffin coated aluminum sheet floated on a refrigerated circulating ethanol bath kept at -5° C. The fraction of the drops that had frozen after 3 minutes was determined visually by distinguishing supercooled liquid drops from those which had turned to ice.
- N The number (N) of ice nuclei per unit volume was calculated from the fraction (F) of droplets frozen (and accounting for the dilution of the original sample) by applying the following formula-
- the mixtures of NSA and HML Silco surprisingly exhibited superior ability to inhibit bacterial ice nucleation.
- the high activity associated with these is believed to be due to a synergistic effect.
- dilute solutions of either NSA alone, or HML Silco alone had little to no ability to reduce bacterial ice nucleation.
- dilute mixtures of NSA and HML Silco demonstrated substantial ability to suppress or eliminate bacterial ice nucleation.
- the surprising suppression of bacterial ice nucleation is due to dissociation or denaturation of ice nucleating proteins associated with the bacteria, and/or a disruption of the bacterial membrane fluidity such that the proteins have less ability to nucleate ice. It is also considered likely that the suppression of ice nucleation is due to bacterial cell membrane perturbation. It is further considered likely that at least the Test Samples may have intercalated in, or otherwise disrupted, the outer bacterial membrane, thereby destabilizing the ice protein aggregates normally involved in ice formation. It is also considered likely that surfactant properties of the Test Samples were effective in perturbing bacterial membranes.
- the “Inhibition” column records the shift between the T50 value of the reference/control and the Composition.
- compositions 7 and 10 showed the best inhibition of ice nucleation, with inhibition of more than 4°C.
- Trial A and Trial B differed in that Trial A was run at a constant -5°C, while Trial B was run over a range of temperatures. Also, Trial A was undertaken against a 31 R1 isolate of Pseudomonas syringae syringae, whereas Trial B was run against refined proteins of the same bacterial isolate. However, the results of both trials show the similar trends in efficacy.
- This trial was conducted using the same methodology described for Trial A. The purpose of it was to assess the ability of three fatty acids of shorter or longer carbon chain length, of similar concentration, alone and in combination with a common silicate composition, to suppress ice nucleation caused by the bacteria Pseudomonas syringae pv Syringae strain 31 R1. This strain is considered particularly active in causing ice nucleation.
- Test Solutions were prepared for HML Silco and NSA as noted above.
- Test Solutions were also prepared for N1 and N2 as follows -
- Test Solutions were used as, or made up into, Test Samples as follows -
- the Bacterial Suspension was added to each Test Sample 30 minutes after it had been formed, to a concentration of 10 7 cells per mL. Then, after 15 minutes, 40 x 10 pL droplets of each Test Sample (which by then included the Bacterial Suspension) were placed on the surface of a paraffin-coated aluminum sheet that was floated on a refrigerated circulating ethanol bath kept at -5° C. The fraction of the drops that had frozen after 3 minutes was determined visually by distinguishing supercooled liquid drops from those which had turned to ice. It was observed that no further drops froze even when the incubation time was extended beyond the 3 minutes to allow for the drops to equilibrate to the temperature of the circulating ethanol.
- N The number (N) of ice nuclei per unit volume was calculated from the fraction (F) of droplets frozen (and accounting for the dilution of the original sample) by applying the following formula-
- Figure 1 , Figure 2 and Figure 3 illustrate the results graphically for each of the different rates of HML Silco.
- the y-axis is a logarithmic scale.
- Test Solutions were prepared for HML Silco and N1 as described above.
- a Test Solution was also prepared for Na1 , as follows -
- Test Solutions were used as, or made up into, Test Samples as follows -
- N1 and Na1 were made in the same way to the same fatty acid concentration, using the same vegetable oil, to have the same hydroxide ratio (the amount of sodium hydroxide versus potassium hydroxide was varied to account for the different atomic weight thereof).
- the Bacterial Suspension was added to each Test Sample 30 minutes after the solution had been formed, to a concentration of 10 7 cells per mL. Then, after 15 minutes, 40 x 10 pL droplets of each Test Sample (which by then incorporated the Bacterial Suspension) were placed on the surface of a paraffin-coated aluminum sheet floated on a refrigerated circulating ethanol bath kept at -5° C. The fraction of the drops that had frozen after 3 minutes was determined visually by distinguishing supercooled liquid drops from those which had turned to ice.
- N The number (N) of ice nuclei per unit volume was calculated from the fraction (F) of droplets frozen (and accounting for the dilution of the original sample) by applying the following formula-
- Figure 4 show the results graphically for the potassium and sodium salts of the fatty acids.
- the y-axis is a logarithmic scale.
- the Trial results show that for each rate of HML Silco, the soaps having different metallic ions performed similarly.
- Test Solutions were prepared for HML Silco (Modulus 2.23) and NSA in the same way described above.
- Test Solutions were made for HML Silco, NSA, PS2 and PS3, which were composed as follows-
- Test Solutions were used as, or made up into, Test Samples as follows - For the ‘combination’ Test Samples (Nos 13 - 39), the silicate (HML Silco [also called PS1], PS2 and PS3) was added to the water prior to the NSA.
- HML Silco also called PS1]
- PS2 silicate
- the silica concentrations of the three potassium silicates were different. However they were adjusted to a similar concentration on the basis of their silica dioxide levels.
- the Bacterial Suspension was added to the Test Samples 30 minutes after it had been formed, to a concentration of 10 7 cells per mL. Then, after 15 minutes, 40 x 10 pL droplets of each Test Sample (which by then incorporated the Bacterial Suspension) were placed on the surface of a paraffin-coated aluminum sheet floated on a refrigerated circulating ethanol bath kept at -5° C. The fraction of the drops that had frozen after 3 minutes was determined visually by distinguishing supercooled liquid drops from those which had turned to ice.
- N The number (N) of ice nuclei per unit volume was calculated from the fraction (F) of droplets frozen (and accounting for the dilution of the original sample) by applying the following formula-
- Figure 7, Figure 8 and Figure 9 illustrate the results graphically for each of the different moduli of silicate.
- the y-axis is a logarithmic scale.
- Test Solutions were prepared for PS3, S1 , L1 and NSA as follows -
- Test Solutions were used as, or made up into, Test Samples as follows -
- silica concentrations of the three potassium silicates were different. However they were adjusted to a comparable concentration based on their silica dioxide levels.
- the Bacterial Suspension was added to each Test Sample 30 minutes after it had been formed, to a concentration of 10 7 cells per mL. Then, after 15 minutes, 40 x 10 pL droplets of each Test Sample (which by then incorporated the Bacterial Suspension) were placed on the surface of a paraffin-coated aluminum sheet floated on a refrigerated circulating ethanol bath kept at -5° C. The fraction of the drops that had frozen after 3 minutes was determined visually by distinguishing supercooled liquid drops from those which had turned to ice.
- N The number (N) of ice nuclei per unit volume was calculated from the fraction (F) of droplets frozen (and accounting for the dilution of the original sample) by applying the following formula-
- Figure 10, Figure 11 and Figure 12 illustrate the results more graphically for each of the different metals of silicate when combined with different rates of NSA respectively.
- the y-axis is a logarithmic scale.
- silicates While not all silicates were trialled, it is considered that at other silicates, including those that are commercially available, will work effectively, alone or in combinations.
- fatty acids and silicates may be used in a similar manner to that described above for protecting plants against ice nucleation induced frost damage caused by other bacteria.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Forests & Forestry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
De nombreuses plantes sont sujettes à des dommages causés par le gel à basses températures. Les dommages ont tendance à être causés par la formation de glace dans le tissu végétal. Un objet d'une forme préférée de l'invention vise à résoudre ledit problème. À cet effet, l'invention concerne un procédé de protection d'une plante contre les dommages causés par le gel, comprenant l'application à l'extérieur de la plante (a) d'acide gras en solution ou en suspension et (b) de silicate en solution ou en suspension, en une quantité suffisante pour interférer de manière protectrice avec la nucléation de la glace induite par des bactéries.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ78066121 | 2021-09-27 | ||
NZ780661 | 2021-09-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2023048581A1 true WO2023048581A1 (fr) | 2023-03-30 |
Family
ID=85720996
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/NZ2022/050118 WO2023048581A1 (fr) | 2021-09-27 | 2022-09-02 | Protection de plantes contre les dommages causés par le gel |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2023048581A1 (fr) |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103408378A (zh) * | 2013-08-27 | 2013-11-27 | 安徽农业大学 | 一种预防小麦低温冻害的专用烟雾剂及其制备方法 |
CN106942272A (zh) * | 2017-03-01 | 2017-07-14 | 特斯联(北京)科技有限公司 | 一种智慧园林花卉抗寒剂及其制备方法 |
CN108056114A (zh) * | 2017-12-22 | 2018-05-22 | 苏州萨瑟斯兰环境科技有限公司 | 一种提高植物耐冻性的组合物及其制备方法 |
CN109111257A (zh) * | 2018-10-19 | 2019-01-01 | 长沙协浩吉生物工程有限公司 | 一种果树专用生物灌根肥的配制方法 |
US10492356B2 (en) * | 2016-07-06 | 2019-12-03 | Crop Enhancement, Inc. | Nontoxic coating concentrates for agricultural uses |
WO2020096466A1 (fr) * | 2018-11-05 | 2020-05-14 | Henry Manufacturing Limited | Traitement de plantes ou de champignons contre une maladie |
WO2020149749A1 (fr) * | 2019-01-14 | 2020-07-23 | Henry Manufacturing Limited | Traitement de plantes contre une maladie |
KR20210033137A (ko) * | 2019-09-18 | 2021-03-26 | 주식회사 단양클로렐라 | 냉해방지 및 뿌리발근 효과가 우수한 클로렐라 조성물의 제조방법 |
-
2022
- 2022-09-02 WO PCT/NZ2022/050118 patent/WO2023048581A1/fr unknown
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103408378A (zh) * | 2013-08-27 | 2013-11-27 | 安徽农业大学 | 一种预防小麦低温冻害的专用烟雾剂及其制备方法 |
US10492356B2 (en) * | 2016-07-06 | 2019-12-03 | Crop Enhancement, Inc. | Nontoxic coating concentrates for agricultural uses |
CN106942272A (zh) * | 2017-03-01 | 2017-07-14 | 特斯联(北京)科技有限公司 | 一种智慧园林花卉抗寒剂及其制备方法 |
CN108056114A (zh) * | 2017-12-22 | 2018-05-22 | 苏州萨瑟斯兰环境科技有限公司 | 一种提高植物耐冻性的组合物及其制备方法 |
CN109111257A (zh) * | 2018-10-19 | 2019-01-01 | 长沙协浩吉生物工程有限公司 | 一种果树专用生物灌根肥的配制方法 |
WO2020096466A1 (fr) * | 2018-11-05 | 2020-05-14 | Henry Manufacturing Limited | Traitement de plantes ou de champignons contre une maladie |
WO2020149749A1 (fr) * | 2019-01-14 | 2020-07-23 | Henry Manufacturing Limited | Traitement de plantes contre une maladie |
KR20210033137A (ko) * | 2019-09-18 | 2021-03-26 | 주식회사 단양클로렐라 | 냉해방지 및 뿌리발근 효과가 우수한 클로렐라 조성물의 제조방법 |
Non-Patent Citations (5)
Title |
---|
AZAD H, SCHAAD N.W: "The Relationship ofXanthomonas campestris pv. translucens to Frost and the Effect of Frost on Black Chaff Development in Wheat", PHYTOPATHOLOGY, AMERICAN PHYTOPATHOLOGICAL SOCIETY, US, vol. 78, no. 1, 1 January 1988 (1988-01-01), US, pages 95 - 99, XP009544912, ISSN: 2813-2424, DOI: 10.1094/Phyto-78-95 * |
BIDDULPH BEN, ET AL.: "Bacterial Ice Nucleation Activity In Rainfall And On Crop Residues May Explain Why Pre-Frost Rainfall And Stubble Retention Increase Frost Damage In WA Cropping Systems", GRDC.COM.AU, AU, 14 April 2021 (2021-04-14), AU, pages 1 - 7, XP093055838, Retrieved from the Internet <URL:https://grdc.com.au/resources-and-publications/grdc-update-papers/tab-content/grdc-update-papers/2021/02/bacterial-ice-nucleation-activity-in-rainfall-and-on-crop-residues-may-explain-why-pre-frost-rainfall-and-stubble-retention-increase-frost-damage-in-wa-cropping-systems> [retrieved on 20230620] * |
MITTELSTÄDT H., RUDOLPH K.: "Ice Nucleation Activity of Strains from Pseudomonas syringae Pathovars atrofadens and syringae, Mainly Isolated from Cereals", JOURNAL OF PHYTOPATHOLOGY - PHYTOPATHOLOGISCHE ZEITSCHRIFT., WILEY-BLACKWELL VERLAG GMBH, DE, vol. 146, no. 11-12, 1 December 1998 (1998-12-01), DE , pages 581 - 586, XP093055835, ISSN: 0931-1785, DOI: 10.1111/j.1439-0434.1998.tb04758.x * |
PAN XUE, LI HAIYING, CHEN DINGFANG, ZHENG JINJIN, YIN LONGHUA, ZOU JUAN, ZHANG YALUN, DENG KAIWEN, XIAO MEIFENG, MENG LEI, HE FUYU: "Comparison of Essential Oils of Houttuynia cordata Thunb. from Different Processing Methods and Harvest Seasons Based on GC-MS and Chemometric Analysis", INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY, vol. 2021, 16 July 2021 (2021-07-16), pages 1 - 13, XP093055831, ISSN: 1687-8760, DOI: 10.1155/2021/8324169 * |
SHIVRAJ HARIRAM NILE: "The nutritional, biochemical and health effects of makgeolli - a traditional Korean fermented cereal beverage : Makgeolli - a traditional fermented cereal beverage", JOURNAL OF THE INSTITUTE OF BREWING, INSTITUTE OF BREWING. LONDON., GB, vol. 121, no. 4, 1 October 2015 (2015-10-01), GB , pages 457 - 463, XP055431948, ISSN: 0046-9750, DOI: 10.1002/jib.264 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Liu et al. | Sub-zero temperature preservation of fruits and vegetables: A review | |
Ruiz-Cruz et al. | Efficacy of sanitizers in reducing Escherichia coli O157: H7, Salmonella spp. and Listeria monocytogenes populations on fresh-cut carrots | |
Lyons et al. | The plant membrane in response to low temperature: an overview | |
Teixidó et al. | Biological control of postharvest diseases in fruit and vegetables | |
Chandra et al. | Effects of household processing on reduction of pesticide residues in brinjal and okra | |
CN101233852B (zh) | 脂肪酸组合物 | |
Wu | An overview of postharvest biology and technology of fruits and vegetables | |
AU2002320237A1 (en) | Preservation compositions and process for mushrooms | |
RU2317703C1 (ru) | Способ криоконсервирования спермы осетровых рыб | |
Hanani et al. | Effect of chitosan-palm stearin edible coating on the post harvest life of star fruits (Averrhoa carambola L.) stored at room temperature. | |
CN103392793B (zh) | 大熊猫食用竹笋的保鲜技术 | |
CN103125589B (zh) | 一种冷敏型果蔬采后抗冷防腐保鲜剂及其应用技术 | |
Hajilou et al. | Effects of post-harvest calcium chloride or salicylic acid treatments on the shelf-life and quality of apricot fruit | |
WO2002045530A2 (fr) | Composition destinee au nettoyage des fruits, des legumes et des surfaces en contact avec les aliments | |
WO2023048581A1 (fr) | Protection de plantes contre les dommages causés par le gel | |
EP3445167B1 (fr) | Cryopréservation de phases juveniles des cirripèdes | |
AU2019377010B2 (en) | Treatment of plants or fungi against disease | |
US20100086658A1 (en) | low fat consumer product comprising a natural preservative system and a method for making the same | |
JP2011503090A (ja) | 発芽抑制剤としてのフッ化スルフリルの使用 | |
US20020176896A1 (en) | Frozen biocidal compositions and methods of use thereof | |
Belwal et al. | Postharvest chilling injury in fruits and vegetables and its alleviation | |
Shumye et al. | Effect of integrated postharvest handling practices on quality and shelf-life of cactus pear [Opuntia ficus-indica (L.) Mill.] fruits | |
JPH09252719A (ja) | カット野菜・カット果物の鮮度保持剤 | |
JPS6024152A (ja) | 青果物の鮮度保持方法 | |
Saito et al. | Sensitivity of Mucor piriformis to Natamycin and Efficacy of Natamycin Alone and with Salt and Heat Treatments Against Mucor Rot of Stored Mandarin Fruit |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 22873261 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |