WO2023048377A1 - Système d'amplification isotherme à canaux multiples - Google Patents

Système d'amplification isotherme à canaux multiples Download PDF

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Publication number
WO2023048377A1
WO2023048377A1 PCT/KR2022/010737 KR2022010737W WO2023048377A1 WO 2023048377 A1 WO2023048377 A1 WO 2023048377A1 KR 2022010737 W KR2022010737 W KR 2022010737W WO 2023048377 A1 WO2023048377 A1 WO 2023048377A1
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WO
WIPO (PCT)
Prior art keywords
heating block
isothermal amplification
amplification system
sample
channel
Prior art date
Application number
PCT/KR2022/010737
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English (en)
Korean (ko)
Inventor
김수경
구자령
남동훈
이동철
장성욱
정도현
Original Assignee
주식회사 나노바이오라이프
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Publication of WO2023048377A1 publication Critical patent/WO2023048377A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • the present invention relates to a multi-channel isothermal amplification system, and relates to a multi-channel isothermal amplification system capable of obtaining results more quickly and efficiently through detection through light of various wavelengths.
  • Nucleic acid amplification technology is a technique mainly used in the fields of molecular biology and biotechnology, and is a method capable of detecting and analyzing a small amount of nucleic acid.
  • PCR Polymerase Chain Reaction
  • Conventional PCR repeats the process of separating double-stranded DNA into single-stranded DNA under high temperature conditions, lowering the temperature, binding the primer to the single-strand, and elongating it into double-stranded DNA by Taq polymerase.
  • Real-time PCR is widely used in performing nucleic acid analysis because it enables real-time confirmation and quantitative analysis during a reaction cycle without performing electrophoresis on a nucleic acid amplified product on a gel.
  • the present invention has been devised to solve the above problems, and the multi-channel isothermal amplification system according to the present invention enables detection through light of various wavelengths through a multi-channel optical system for a plurality of sample tubes in a heating block. It is intended to be able to obtain accurate result values for multiple samples more quickly and efficiently.
  • the multi-channel isothermal amplification system arranges a light source module at the bottom of the heating block where the sample tube is located, and arranges a detection module for detecting the fluorescent signal of the sample on the side of the heating block, so that the light source module and It is intended to provide a multi-channel isothermal amplification system capable of more accurate measurement by preventing mutual interference of light between the detection modules.
  • the multi-channel isothermal amplification system according to the present invention is intended to further improve usability and portability by integrating a light source module and a detection module into a more compact configuration.
  • a multi-channel isothermal amplification system for solving the above problems includes a heating block in which holes into which a plurality of sample tubes are inserted are formed in a row; an optical system for detecting fluorescence signals of samples in the sample tubes by incident light of different wavelengths on each of the sample tubes inserted into the heating block; a heat sink dissipating heat from the heating block to the outside; and a peltier module disposed between the heating block and the heat sink to exchange heat between the heating block and the heat sink.
  • the optical system includes a light source module for incident light from the lower portion of the heating block toward the sample tube; and a detection module for detecting a fluorescent signal from the sample at a side of the heating block.
  • the heat dissipation plate may be disposed on the other side opposite to the side on which the detection module is disposed to emit heat from the heating block to the outside.
  • the light source module LED for irradiating light; an excitation filter filtering light emitted from the LED; and a first lens concentrating the light filtered by the excitation filter onto a sample in the sample tube.
  • the detection module includes an emission filter for receiving a fluorescence signal of the sample; a second lens that transmits the fluorescence signal filtered by the emission filter; and an optical sensor for receiving a fluorescence signal introduced through the second lens.
  • the heating block includes a first heating block region in which a plurality of the holes are formed in a line; and a second heating block area in which the plurality of holes are formed in a line and spaced apart from each other by a gap between the holes in the longitudinal direction of the first heating block area, and disposed in a line with the first heating block area.
  • a first heating block region in which a plurality of the holes are formed in a line
  • a second heating block area in which the plurality of holes are formed in a line and spaced apart from each other by a gap between the holes in the longitudinal direction of the first heating block area, and disposed in a line with the first heating block area.
  • each channel of the optical system moves in the longitudinal direction of the first heating block area and the second heating block area arranged in a line, and each of the optical systems moves in the first heating block area. and by sequentially incident light having a set wavelength to each of the sample tubes inserted into the holes of the second heating block region, the fluorescence signal of the sample in the sample tube may be detected.
  • the multi-channel isothermal amplification system enables detection through light of various wavelengths through a multi-channel optical system for a plurality of sample tubes in a heating block, so that accurate results for multiple samples can be obtained more quickly and efficiently. can be obtained
  • the multi-channel isothermal amplification system arranges a light source module at the bottom of the heating block where the sample tube is located, and arranges a detection module for detecting the fluorescent signal of the sample on the side of the heating block, so that the light source module and It is possible to provide a multi-channel isothermal amplification system capable of more accurate measurement by preventing mutual interference of light between the detection modules.
  • the multi-channel isothermal amplification system according to the present invention can further improve usability and portability by integrating a light source module and a detection module into a more compact configuration.
  • FIG. 1 is a diagram showing a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 2 is an internal perspective view of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 3 is a diagram showing a heating block of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 4 is a diagram for explaining in detail the configuration of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 5 is a diagram for explaining an operating method of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 6 is a block diagram of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 1 is a diagram showing a multi-channel isothermal amplification system according to an embodiment of the present invention
  • FIG. 2 is an internal perspective view of the multi-channel isothermal amplification system according to an embodiment of the present invention.
  • FIG. 3 is a diagram showing a heating block of a multi-channel isothermal amplification system according to an embodiment of the present invention
  • FIG. 4 describes the configuration of the multi-channel isothermal amplification system according to an embodiment of the present invention in more detail.
  • 5 is a diagram for explaining an operating method of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • a multi-channel isothermal amplification system includes a heating block 100, an optical system 200, a heat sink 300, and a Peltier module 400.
  • Holes 102 into which a plurality of sample tubes 101 are inserted are formed in the heating block 100, and the holes 102 are also referred to as wells. At this time, the holes 102 are formed in a line. That is, the holes 102 are formed in a line in one direction on the heating block 100, and sample tubes 101 accommodating samples may be disposed in the holes 102, respectively.
  • the heating block 100 may be divided into a first heating block area 100A and a second heating block area 100B.
  • the first heating block area 100A has a plurality of holes 102 formed in a line, and similarly, a plurality of holes 102 are formed in a line in the second heating block area 100B. 2
  • the heating block area 100B may be spaced apart from the first heating block area 100A by the distance between the hole 102 and the hole 102, and may be disposed in line with the first heating block area 100A. there is.
  • the optical system 200 injects light of each wavelength into the sample tubes 101 inserted into the heating block 100, and detects a fluorescence signal of the sample in the sample tubes 101.
  • the optical system 200 is configured to include four channels, and each channel incidents light of a different wavelength to the sample tube 101 inserted into the heating block 100, respectively. and detecting the fluorescence signal of the sample in the sample tube 101 and moving sequentially.
  • each channel of the optical system 200 may include a light source module 210 and a detection module 220 .
  • the light source module 210 injects light from the bottom of the heating block 100 toward the sample tube 101, and the detection module 220 emits a fluorescent signal from the sample at the side of the heating block 100. can be detected.
  • the light source module 210 includes an LED 211 for emitting light, an excitation filter 212 for filtering light emitted from the LED 211, and a filter filtered by the excitation filter 212. It may be configured to include a first lens 213 for concentrating light onto the sample in the sample tube 101.
  • the detection module 220 includes an emission filter 221 for receiving the fluorescence signal of the sample, a second lens 222 for transmitting the fluorescence signal filtered by the emission filter 221, and the second It may be configured to include an optical sensor 223 that receives a fluorescence signal introduced through the lens 222 .
  • the heat sink 300 dissipates heat from the heating block 100 to the outside.
  • the heat dissipation plate 300 is disposed on the other side opposite to the side where the detection module 220 is disposed, so that heat from the heating block 100 can be effectively discharged to the outside.
  • the heat sink 300 includes a first heat sink disposed to correspond to the first heating block area 110A of the heating block 100, and a second heating block area 110B of the heating block 100. It may be composed of a second heat dissipation plate disposed to correspond to.
  • the Peltier module 400 may be disposed between the heating block 100 and the heat sink 300 to exchange heat between the heating block 100 and the heat sink 300 .
  • the multi-channel isothermal amplification system effectively cools the heat from the heating block 100 through the Peltier module 400 and more effectively releases it through the heat sink 300 at the same time.
  • the optical system 200 composed of the four channels is the first heating block region 100A of the heating block 100 and moves in the longitudinal direction of the second heating block area 100B, and each channel of the optical system 200 is inserted into the holes of the first heating block area 100A and the second heating block area 100B.
  • a fluorescence signal of a sample in the sample tube 101 may be detected by sequentially incident light having a set wavelength to each of the sample tubes 101 .
  • the multi-channel isothermal amplification system can obtain accurate results more quickly and efficiently by detecting fluorescence signals through four channels for a plurality of sample tubes in the heating block.
  • an embodiment of an optical system including four channels has been described, but the number of channels of the optical system can be changed as needed to achieve more rapid and efficient detection.
  • the multi-channel isothermal amplification system arranges a light source module at the bottom of a heating block where a sample tube is located and a detection module for detecting a fluorescent signal of a sample on the side of the heating block, so that the light source It is possible to provide a multi-channel isothermal amplification system capable of more accurate measurement by preventing mutual interference of light between the module and the detection module.
  • FIG. 6 is a block diagram of a multi-channel isothermal amplification system according to an embodiment of the present invention.
  • the multi-channel isothermal amplification system is configured to include an LCD touch panel, and inputs a user's control command through the LCD touch panel and detects a sample signal. Information and related information can be displayed.
  • the multi-channel isothermal amplification system operates a main controller, a motor driver for operating a stepper motor, and an LED of an optical module.
  • LED driver for operating a stepper motor
  • LED of an optical module for detecting the heat of the heating block 100
  • Peltier control for controlling the Peltier module
  • top heater installed in the heating block ), including a heater control (Heater Control), a temperature sensor (Temperature Sensor) installed in the top heater, and a fan (Fan), and is controlled by the main controller.
  • the multi-channel isothermal amplification system according to the present invention can further improve usability and portability by integrating the light source module and the detection module into a more compact configuration.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Biomedical Technology (AREA)

Abstract

La présente invention concerne un système d'amplification isotherme à canaux multiples comprenant : un bloc chauffant dans lequel des trous où sont insérés plusieurs tubes à échantillons sont formés en une rangée ; un système optique pour détecter des signaux de fluorescence d'échantillons dans les tubes à échantillons insérés dans le bloc chauffant en émettant une lumière d'une longueur d'onde différente sur chacun des tubes à échantillons ; un dissipateur thermique pour dissiper la chaleur du bloc chauffant vers l'extérieur ; et un module Peltier qui est disposé entre le bloc chauffant et le dissipateur thermique et qui échange de la chaleur entre le bloc chauffant et le dissipateur thermique.
PCT/KR2022/010737 2021-09-24 2022-07-21 Système d'amplification isotherme à canaux multiples WO2023048377A1 (fr)

Applications Claiming Priority (2)

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KR10-2021-0126305 2021-09-24
KR1020210126305A KR102666561B1 (ko) 2021-09-24 2021-09-24 다채널 등온 증폭 시스템

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040048754A (ko) * 2002-12-04 2004-06-10 뮤앤바이오 주식회사 온도 제어가 가능한 리얼타임 형광 검색 장치
JP3776377B2 (ja) * 2002-05-20 2006-05-17 アロカ株式会社 検体検査装置
KR20170074662A (ko) * 2015-12-22 2017-06-30 조원창 유전자 진단 장치
KR102126032B1 (ko) * 2013-07-31 2020-07-08 삼성전자주식회사 다채널 형광 검출 모듈 및 이를 포함하는 핵산 분석 시스템
KR20200143639A (ko) * 2019-06-13 2020-12-24 크레도 다이어그노스틱스 바이오메디컬 피티이. 엘티디. 하나 이상의 형광 신호에 대한 실시간 검출이 가능한 중합효소 연쇄 반응 장치

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100580639B1 (ko) 2003-12-30 2006-05-16 삼성전자주식회사 미세유체 검출을 위한 형광검출기
KR20210029449A (ko) * 2019-09-06 2021-03-16 한국전자기술연구원 등온 증폭을 이용한 소형 진단 시스템

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3776377B2 (ja) * 2002-05-20 2006-05-17 アロカ株式会社 検体検査装置
KR20040048754A (ko) * 2002-12-04 2004-06-10 뮤앤바이오 주식회사 온도 제어가 가능한 리얼타임 형광 검색 장치
KR102126032B1 (ko) * 2013-07-31 2020-07-08 삼성전자주식회사 다채널 형광 검출 모듈 및 이를 포함하는 핵산 분석 시스템
KR20170074662A (ko) * 2015-12-22 2017-06-30 조원창 유전자 진단 장치
KR20200143639A (ko) * 2019-06-13 2020-12-24 크레도 다이어그노스틱스 바이오메디컬 피티이. 엘티디. 하나 이상의 형광 신호에 대한 실시간 검출이 가능한 중합효소 연쇄 반응 장치

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KR20230043438A (ko) 2023-03-31

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