WO2016017832A1 - Dispositif d'amplification de l'acide nucleique - Google Patents

Dispositif d'amplification de l'acide nucleique Download PDF

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Publication number
WO2016017832A1
WO2016017832A1 PCT/KR2014/006966 KR2014006966W WO2016017832A1 WO 2016017832 A1 WO2016017832 A1 WO 2016017832A1 KR 2014006966 W KR2014006966 W KR 2014006966W WO 2016017832 A1 WO2016017832 A1 WO 2016017832A1
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid amplification
constant temperature
reaction tube
block
Prior art date
Application number
PCT/KR2014/006966
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English (en)
Korean (ko)
Inventor
이현영
Original Assignee
이현영
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 이현영 filed Critical 이현영
Priority to PCT/KR2014/006966 priority Critical patent/WO2016017832A1/fr
Publication of WO2016017832A1 publication Critical patent/WO2016017832A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control

Definitions

  • the present invention relates to a nucleic acid amplification apparatus, and more particularly, to a nucleic acid amplification apparatus having increased nucleic acid amplification efficiency by alternately contacting a thermoblock having different temperatures to a nucleic acid amplification reagent mixture.
  • nucleic acid amplification technology is a very important technique for molecular gene analysis, and is used as an important technique for analysis of human genome research, detection of infectious and genetic diseases, and the like.
  • the PCR (Polymerase Chain Reaction) method which is a representative technique of the nucleic acid amplification technology, is the most widely known technique, and is a technology capable of selectively amplifying a large amount of a specific nucleic acid sequence only.
  • the general PCR method amplifies a specific sequence in a large amount by repeating the process of denaturation and extension extension. Therefore, in order to repeat this process of denaturation hybridization, the nucleic acid amplification reagents must be changed to reach the respective set temperatures.
  • the most commonly used for the temperature repetition is a Peltier device, and a device for amplifying nucleic acids by heating and cooling the temperature cycle in one block is typically used.
  • the nucleic acid amplification apparatus of the type that repeats the heating and cooling of the block itself has problems such as high precision temperature control technology and time required for raising and lowering the temperature, resulting in complicated equipment structure and increased reaction time.
  • Korean Patent Publication No. 10-2012-0075918 multi-chamber reactor for nucleic acid amplification and analysis.
  • the present invention is to provide a nucleic acid amplification apparatus capable of amplifying nucleic acids more quickly and simply by allowing two incubating blocks to be in contact with the nucleic acid amplification solution.
  • a fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20;
  • a high temperature constant temperature block 14 coupled to one side of the moving table 13;
  • a nucleic acid amplification apparatus including a power module 16 capable of moving the movement table 13 to the left and right is provided.
  • the fixing portion 12 is a body portion hole formed in the direction of the chamber 111 from the upper portion of the body portion 11,
  • the reaction tube module 20 The reaction tube module 20,
  • reaction tube units 21 capable of containing a nucleic acid amplification material
  • a frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively;
  • a nucleic acid amplification apparatus comprising a cover member 23 that can cover the plurality of reaction tube unit 21.
  • the high temperature constant temperature block 14 is formed with a plurality of grooves 141,
  • the groove 141 has an upper side and a side surface of the low temperature constant temperature block direction 15,
  • the high temperature constant temperature block 14 is provided with a nucleic acid amplification apparatus, characterized in that the heating device 142 is coupled.
  • a plurality of grooves 151 are formed in the low temperature constant temperature block 15,
  • the groove 151 has an upper side and a side surface in the high temperature constant temperature block 14 direction,
  • the low temperature constant temperature block 15 is provided with a nucleic acid amplification apparatus, characterized in that the heating device 152 and the cooling device 153 is coupled.
  • the present invention provides a nucleic acid amplification apparatus capable of amplifying nucleic acids more quickly and simply by alternately contacting two thermoblocks (high temperature incubation block and low temperature incubation block) with a nucleic acid amplification solution.
  • FIG. 1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention.
  • Figure 2 is a perspective view showing a process of inserting a reaction tube module containing a nucleic acid amplification solution in a nucleic acid amplification apparatus according to an embodiment of the present invention.
  • Figure 3 is a cross-sectional view of the nucleic acid amplification apparatus according to an embodiment of the present invention.
  • FIG. 4 is a perspective view of a drive module according to an embodiment of the present invention.
  • Figure 5 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (the reaction tube module is in contact with the high temperature constant temperature block).
  • Figure 6 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (the reaction tube module is in contact with the low temperature constant temperature block).
  • Figure 7 is an exploded perspective view of the reaction tube module according to an embodiment of the present invention.
  • FIG. 8 is a perspective view of a reaction tube module according to an embodiment of the present invention.
  • a nucleic acid amplification apparatus including a power module 16 capable of moving 13) left and right.
  • FIG. 1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention
  • Figure 2 is a perspective view showing a process of inserting a reaction tube module containing a nucleic acid amplification solution in a nucleic acid amplification apparatus according to an embodiment of the present invention
  • 3 is a cross-sectional view of a nucleic acid amplification apparatus according to an embodiment of the present invention
  • Figure 4 is a perspective view of a driving module according to an embodiment of the present invention
  • Figure 5 is a nucleic acid amplification apparatus according to an embodiment of the present invention
  • a cross-sectional view of the drive module (the reaction tube module is in contact with the high temperature constant temperature block)
  • Figure 6 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (reaction tube module is in contact with the low temperature constant temperature block)
  • 7 is an exploded perspective view of a reaction tube module according to an embodiment of the present
  • the body portion 11 is formed with a chamber 111; A fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20; a moving table 13 coupled to the inside of the chamber 111; and the moving table 13
  • the high temperature constant temperature block 14 coupled to one side of the; and
  • the low temperature constant temperature block 15 coupled to the other side of the moving table 13, disposed to face the high temperature constant temperature block 14; 13) includes a power module 16 capable of moving left and right.
  • the body portion 11 forms the appearance of the nucleic acid amplification apparatus 10, and various electronic components and electronic devices are embedded therein, and a display device may be additionally coupled to the outside. In addition, the body portion 11 may be coupled to the input module for operating the nucleic acid amplification device (10).
  • the chamber 111 is formed inside the body portion 11.
  • the reaction tube module 20 may be coupled to the inside of the chamber 111.
  • a fixing part 12 to which the reaction tube module 20 is fixed is formed in the center of the chamber 111.
  • the fixing part 12 may be a body hole formed in the body part 11. As the upper portion of the reaction tube module 20 spans the body hole, most of the reaction tube module 20 enters into the chamber 111.
  • the moving table 13 is coupled to the inside of the chamber 111.
  • the moving table 13 is preferably horizontally coupled to the chamber 111.
  • the movement table 13 may reciprocate left and right by the operation of the power module 16 (see FIG. 3).
  • the high temperature constant temperature block 14 is coupled to one side of the upper portion of the moving table 13.
  • the low temperature constant temperature block 15 is coupled to the other side of the upper portion of the moving table 13.
  • the low temperature constant temperature block 15 is disposed to face the high temperature constant temperature block 14.
  • the high temperature constant temperature block 14 is preferably maintained at about 90 degrees Celsius to about 95 degrees Celsius.
  • a plurality of grooves 141 are formed in the high temperature constant temperature block 14, and the groove 141 has an upper side and a side surface of the low temperature constant temperature block 15.
  • the heating device 142 is coupled to the high temperature constant temperature block 14.
  • the heat generator 142 may be a Peltier device.
  • the low temperature constant temperature block 15 is preferably maintained at about 50 degrees Celsius to about 70 degrees Celsius.
  • a plurality of grooves 151 are formed in the low temperature constant temperature block 15, and the groove 151 has an open side and a side in the direction of the high temperature constant temperature block 14, and the heat generating device 152 in the low temperature constant temperature block 15.
  • the cooling device 153 may be combined. By controlling the heating device 152 and the cooling device 153, the low temperature constant temperature block 15 may be maintained at about 50 degrees Celsius to about 70 degrees Celsius.
  • the reaction tube module 20 may have an upper portion fixed to the fixing portion 12.
  • the fixing part 12 may be a body hole formed in the direction of the chamber 111 from the upper part of the body part 11.
  • the reaction tube module 20 includes: a plurality of reaction tube units 21 capable of containing a nucleic acid amplification material; A frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively; It includes a cover member 23 that can cover a plurality of reaction tube unit 21 (see Fig. 7).
  • the power module 16 is composed of a combination of a motor and a gear, and repeatedly moves the movement table 13 to the left and right by forward rotation of the motor.
  • the moving table 13 may be moved by a combination of a linear gear coupled to the bottom of the movement table 13 and a circular gear gear coupled to a motor.
  • the power module 16 may move the moving table 13 using a chain or a belt.
  • the high temperature constant temperature block 14 is in contact with the reaction tube module 20 to increase the temperature of the nucleic acid amplification material in the reaction tube module 20. Thereafter, after a predetermined time passes, when the power module 16 returns to the counterclockwise direction, as shown in FIG. Combined. As such, while the moving table 13 moves left and right, the reaction tube module 20 is in contact with the high temperature constant temperature block 14 or the low temperature constant temperature block 15. Thus, timely reaction tube module 20 is placed in a high temperature and low temperature environment.
  • the present invention can be utilized in the nucleic acid amplifier industry.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un dispositif d'amplification de l'acide nucléique. L'invention concerne un dispositif d'amplification de l'acide nucléique comportant une partie de corps (11) comprenant une chambre (111); une partie de fixation (12) formée dans le centre de la chambre (111) de sorte qu'un module de tube de réaction (20) y soit fixé; une table mobile (13) couplée à l'intérieur de la chambre (111); un bloc à haute température constante (14) couplé à un côté de la table mobile (13); un bloc à basse température constante (15) couplé à l'autre côté de la table mobile (13) et disposé de manière à être opposé au bloc à haute température constante (14); et un module d'alimentation électrique (16) capable de déplacer la table mobile (13) vers la droite et vers la gauche.
PCT/KR2014/006966 2014-07-30 2014-07-30 Dispositif d'amplification de l'acide nucleique WO2016017832A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR2014/006966 WO2016017832A1 (fr) 2014-07-30 2014-07-30 Dispositif d'amplification de l'acide nucleique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2014/006966 WO2016017832A1 (fr) 2014-07-30 2014-07-30 Dispositif d'amplification de l'acide nucleique

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WO2016017832A1 true WO2016017832A1 (fr) 2016-02-04

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110872557A (zh) * 2018-08-30 2020-03-10 深圳市刚竹医疗科技有限公司 Pcr离心微流控装置及其方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6767512B1 (en) * 1996-11-08 2004-07-27 Eppendorf Ag Temperature-regulating block with temperature-regulating devices
US20070077648A1 (en) * 2005-10-04 2007-04-05 Canon Kabushiki Kaisha Apparatus for performing biochemical processing using container having wells
US8003370B2 (en) * 2006-05-17 2011-08-23 California Institute Of Technology Thermal cycling apparatus
KR20110118572A (ko) * 2010-04-23 2011-10-31 나노바이오시스 주식회사 2개의 열 블록을 포함하는 ｐｃｒ 장치
KR20130008266A (ko) * 2011-07-12 2013-01-22 강원대학교산학협력단 중력유동방식 ｐｃｒ 증폭 장치
KR101427038B1 (ko) * 2013-03-04 2014-08-06 이현영 핵산 증폭장치

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6767512B1 (en) * 1996-11-08 2004-07-27 Eppendorf Ag Temperature-regulating block with temperature-regulating devices
US20070077648A1 (en) * 2005-10-04 2007-04-05 Canon Kabushiki Kaisha Apparatus for performing biochemical processing using container having wells
US8003370B2 (en) * 2006-05-17 2011-08-23 California Institute Of Technology Thermal cycling apparatus
KR20110118572A (ko) * 2010-04-23 2011-10-31 나노바이오시스 주식회사 2개의 열 블록을 포함하는 ｐｃｒ 장치
KR20130008266A (ko) * 2011-07-12 2013-01-22 강원대학교산학협력단 중력유동방식 ｐｃｒ 증폭 장치
KR101427038B1 (ko) * 2013-03-04 2014-08-06 이현영 핵산 증폭장치

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110872557A (zh) * 2018-08-30 2020-03-10 深圳市刚竹医疗科技有限公司 Pcr离心微流控装置及其方法
CN110872557B (zh) * 2018-08-30 2024-04-02 深圳市呈晖医疗科技有限公司 Pcr离心微流控装置及其方法

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