WO2023027423A1 - 원심분리용 피스톤 및 이를 포함하는 원심분리 장치 - Google Patents
원심분리용 피스톤 및 이를 포함하는 원심분리 장치 Download PDFInfo
- Publication number
- WO2023027423A1 WO2023027423A1 PCT/KR2022/012343 KR2022012343W WO2023027423A1 WO 2023027423 A1 WO2023027423 A1 WO 2023027423A1 KR 2022012343 W KR2022012343 W KR 2022012343W WO 2023027423 A1 WO2023027423 A1 WO 2023027423A1
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- WIPO (PCT)
- Prior art keywords
- body surface
- piston
- inner body
- valve
- passage
- Prior art date
Links
- 238000005119 centrifugation Methods 0.000 title abstract description 6
- 238000007789 sealing Methods 0.000 claims abstract description 31
- 238000000926 separation method Methods 0.000 claims description 45
- 239000000126 substance Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 description 28
- 210000001519 tissue Anatomy 0.000 description 14
- 230000005484 gravity Effects 0.000 description 11
- 210000000577 adipose tissue Anatomy 0.000 description 10
- 239000012530 fluid Substances 0.000 description 9
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 230000007704 transition Effects 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 4
- 238000004891 communication Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007443 liposuction Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
- A61M5/315—Pistons; Piston-rods; Guiding, blocking or restricting the movement of the rod or piston; Appliances on the rod for facilitating dosing ; Dosing mechanisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Definitions
- the present disclosure relates to a piston for centrifugal separation and a centrifugal separation device including the same.
- Patent Publication No. 10-2015-0122102 discloses a piston of a syringe for liposuction transplantation.
- One aspect of the present disclosure may provide a centrifugal separation piston for removing impurities from adipose tissue obtained from a living body and obtaining a pure fat mass, and a centrifugal separation device including the same.
- a piston for centrifugal separation is a piston body having an outer body surface and an inner body surface, the piston body including a hollow portion defined by the inner body surface, and the piston body comprising: the outer body a first opening located in a face, a second opening defined by at least a portion of the outer body face and connected to the inner body face and the hollow portion, and a first passage connecting the first opening and the hollow portion.
- a valve configured to open or block the piston body, the first passage, and positioned in the hollow portion spaced apart from the inner body surface, the valve comprising: the first valve between the inner body surface and the outer valve surface.
- an outer valve face defining a passageway and a second passageway in fluid communication with the second opening, the valve including a recessed portion formed in the outer valve face, and positioned in the recessed portion; and a first inner sealing portion configured to contact the inner body surface and to separate the outer valve surface from the inner body surface, and to seal between the inner body surface and the outer valve surface during centrifugal separation.
- the piston may further include a first fixing portion positioned on the inner body surface and configured to limit movement of the valve toward the second opening.
- the valve includes a first valve body portion including the recess portion, and a second valve body portion connected to the first valve body portion, wherein the first fixing portion comprises a surface of the inner body. and a base portion located on the base portion, and a protruding portion projecting from the base portion and configured to contact the first valve body portion.
- the first valve body portion and the protruding portion may contact each other such that a distance between the inner body surface and the outer valve surface is in the range of 5 ⁇ m to 150 ⁇ m.
- the inner body surface includes a first inner body surface and a second inner body surface recessed from the first inner body surface and forming a first step with the first inner body surface, wherein the The first fixing part may be located on the second inner body surface.
- the piston is located on the inner body surface, the second inner seal comprising a first portion and a second portion opposite the first portion, and located on the inner body surface;
- the apparatus may further include a first fixing part configured to support the first part of the second inner sealing part and fix the second inner sealing part to the inner body surface.
- the piston further comprises a second fixing portion positioned on the inner body surface, supporting a second portion of the second inner seal portion, and configured to secure the second inner seal portion to the inner body surface.
- a second fixing portion positioned on the inner body surface, supporting a second portion of the second inner seal portion, and configured to secure the second inner seal portion to the inner body surface.
- the piston body may include a first engagement portion
- the second fixing portion may include a second engagement portion configured to engage with the first engagement portion
- the second fixing part may include a fastening part.
- a centrifugal separation device has a rotating shaft, and the centrifugal separation device is a syringe configured to rotate about the rotating shaft, the syringe including a container capable of holding biological tissue, and an interior of the container.
- a centrifugal piston movably positioned on the piston body, the piston body having an outer body surface and an inner body surface, the piston body including a hollow portion defined by the inner body surface;
- the piston body comprises a first opening located in the outer body face, a second opening defined by at least a portion of the outer body face and connected to the inner body face and the hollow portion, and the first opening and the hollow portion.
- the piston body including a first passage connecting the piston body, a valve configured to open or block the first passage and positioned in the hollow portion spaced apart from the inner body surface, the valve comprising: the inner body surface and the outer the valve having an outer valve face defining a second passage fluidly connected to the first passage and the second opening between the valve faces, wherein the valve includes a recessed portion formed in the outer valve face; and A first inner body positioned in a recess portion, configured to contact the inner body surface and space the outer valve surface away from the inner body surface, and configured to seal between the inner body surface and the outer valve surface during centrifugal separation. A seal may be included.
- the piston comprises a second inner seal located on the inner body surface and comprising a first portion and a second portion opposite to the first portion, located on the inner body surface and comprising the second portion.
- a first fixing portion supporting a first portion of the second inner seal and configured to secure the second inner seal to the inner body surface, positioned on the inner body surface and supporting the second portion of the second inner seal; and a second fixing part configured to fix the second inner sealing part to the surface of the inner body, and a plunger configured to be engaged with the second fixing part, wherein the second inner sealing part contacts the plunger and contacts the inner body. It may be configured to seal between the face and the plunger.
- a piston for centrifugal separation and a centrifugal separation device including the same may provide improved sealing between a space in front of the piston and a space behind the piston while performing centrifugation on adipose tissue obtained from a living body. Effects of the piston for centrifugal separation and the centrifugal separation device including the same according to an embodiment are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below. .
- FIG. 1 is a diagram of a centrifugal separation device according to an embodiment.
- FIG. 2 is a cross-sectional view of a centrifugal separation device according to an embodiment.
- FIG 3 is a perspective view of a piston for centrifugal separation according to an embodiment.
- FIG. 4 is an exploded side view of a piston for centrifugal separation according to an embodiment.
- FIG. 5 is an exploded cross-sectional view of a piston for centrifugal separation according to an embodiment.
- FIG. 6 is a cross-sectional view of a piston body according to one embodiment.
- FIG. 7 is a cross-sectional view of a valve according to one embodiment.
- FIG. 8A is a cross-sectional view of a first fixing unit according to an embodiment.
- FIG. 8B is a plan view of a first fixing unit according to an exemplary embodiment.
- FIG. 9 is a cross-sectional view of a piston for centrifugal separation in a first state according to an embodiment.
- FIG. 10 is a cross-sectional view of a piston for centrifugal separation in a second state according to an embodiment.
- FIG. 11 is a cross-sectional view of a piston for centrifugal separation in a third state according to an embodiment.
- FIG. 12 is a cross-sectional view of a piston and plunger for centrifugal separation according to an embodiment.
- FIGS. 13 to 15 are diagrams illustrating an exemplary operation of a centrifugal separator according to an embodiment.
- first, second, A, B, (a), and (b) may be used. These terms are only used to distinguish the component from other components, and the nature, order, or order of the corresponding component is not limited by the term.
- FIG. 1 is a diagram of a centrifugal separation device according to an embodiment.
- the centrifugal separation device 100 in a centrifugal separation environment, impurities (eg, oil, blood, drug solution, and other impurities) from living tissue (eg, adipose tissue) obtained from a living body (eg, human). ) can be removed and pure adipose tissue can be obtained.
- the centrifugal separator 100 includes a substantially flat base 101, a plurality of cavities 102 arranged in a circumferential direction around the base 101, and a plurality of hinges 103 rotatably connected to the base 101.
- Base 101 may include a rotation axis (X) located substantially in the center of the base (101).
- Each of the plurality of holders 105 can swing into and out of the corresponding cavity 102 by means of a corresponding hinge 103 .
- the plurality of holders 105 may rotate around the axis of rotation X along with the base 101 .
- FIG. 2 is a cross-sectional view of a centrifugal separation device according to an embodiment.
- a centrifugal device 100 may include a syringe 104 configured to rotate about a rotation axis X within a centrifugal environment.
- the syringe 104 is positioned at a cylindrical container 1041 capable of holding biological tissue (BM), a first end (eg, a front end in FIG. 2) of the container 1041, and includes at least one first material ( Example: a connector 1042 configured to pass through and remove blood, drug solution, water, and other substances having a relatively high specific gravity compared to pure fat mass), and a second end opposite to the first end of the container 1041 (eg: It may include a flange 1043 located at the rear end in FIG. 2).
- BM biological tissue
- a connector 1042 configured to pass through and remove blood, drug solution, water, and other substances having a relatively high specific gravity compared to pure fat mass
- a second end opposite to the first end of the container 1041 eg: It may include a flange 1043 located at the rear end in FIG.
- the connector 1042 may include a fastening portion 1042A configured to be coupled to an external removal device (not shown) and a discharge and injection passage 1042B through which the first material passes.
- the second end of the container 1041 on which the flange 1043 is formed may be open to the outside of the syringe 104 .
- the biological tissue (BM) obtained from the living body is placed inside the container 1041 through the discharge and injection passage 1042B, and the piston 106 is placed on the biological tissue (BM) (e.g., on the right side in FIG. 2). can be located.
- the piston 106 may pressurize the biological tissue (BM), and at least one or more second substances (eg, oil and other substances having a relatively small specific gravity compared to pure adipose tissue) are separated from the biological tissue (BM). ) can escape through the inside of the piston 106 toward the second end of the container 1041 in the absence of centrifugal force.
- BM biological tissue
- second substances eg, oil and other substances having a relatively small specific gravity compared to pure adipose tissue
- FIG. 2 is a cross-sectional view of a centrifugal separation device according to an embodiment.
- 3 is a perspective view of a piston for centrifugal separation according to an embodiment.
- 4 is an exploded side view of a piston for centrifugal separation according to an embodiment.
- 5 is an exploded cross-sectional view of a piston for centrifugal separation according to an embodiment.
- 6 is a cross-sectional view of a piston body according to one embodiment.
- 7 is a cross-sectional view of a valve according to one embodiment.
- 8A is a cross-sectional view of a first fixing unit according to an embodiment.
- 8B is a plan view of a first fixing unit according to an exemplary embodiment.
- the centrifugal piston 106 includes a piston body 110, a first outer seal 121, a second outer seal 122, a valve ( 130), a first inner sealing part 140, a second inner sealing part 150, a first fixing part 160 and a second fixing part 170.
- Piston body 110 may be configured to move within vessel 1041 .
- the piston body 110 is configured to move in a direction away from the rotation axis X in a centrifugal separation environment, and may pressurize biological tissue BM obtained from a living body.
- the piston body 110 has an outer body face 111 and an inner body face 114 and may include a hollow portion 113 defined by the inner body face 114 .
- the outer body surface 111 includes a front body surface 111A substantially forming the front of the piston body 110, a side body surface 111B substantially forming a side of the piston body 110, and a piston body ( 110) may include a rear body surface 111C substantially forming at least a portion of the rear.
- the front body surface 111A, the side body surface 111B, and the rear body surface 111C may be integrally and seamlessly formed.
- the front body surface 111A includes a first front body surface 111A-1 and a second front body surface 111A-1 inclined with respect to the first front body surface 111A-1 and the side body surface 111B, respectively. 2) may be included.
- the first front body face 111A-1 is, for example, when the piston 106 is positioned on the portion of the vessel 1041 adjacent to the connector 1042 (e.g., the front portion, the left portion in FIG. 2), Pressurizes the space between the connector 1042 and the piston 106 to facilitate the escape of substances (eg, air, blood, chemical solutions, and water) that may be present in the space through the discharge and injection passage 1042B can do.
- substances eg, air, blood, chemical solutions, and water
- the first front body face 111A-1 and the second front body face 111A-2 compress material in front of the piston 106, for example, when the piston 106 is in a centrifugal environment. and guide the material as the piston 106 moves forward of the syringe 104.
- the first front body surface 111A-1 and the second front body surface 111A-2 may form a substantially truncated cone shape.
- the piston body 110 may include a first opening 115 through which material on the front body surface 111A is introduced into the hollow portion 113 .
- the first opening 115 may be formed, for example, in the second front body surface 111A-2.
- the piston body 110 may include a first passage P1 fluidly connecting the first opening 115 and the hollow portion 113 .
- the first passage P1 may extend in the radial direction of the piston body 110 having a substantially cylindrical shape, for example.
- the piston body 110 may include an external recess 112 formed on the side body surface 111B.
- the external recess 112 may include a first external recess 112A formed in a first portion of the side body surface 111B and a second portion different from the first portion of the side body surface 111B.
- a second external recess 112B may be included.
- the rear body surface 111C may define a second opening 116 in fluid communication with the hollow portion 113 and the exterior of the piston body 110, respectively.
- the second opening 116 may allow materials in the hollow portion 113 to flow out of the piston body 110 .
- the size of the second opening 116 may be larger than that of the first opening 115 .
- the inner body surface 114 includes a first inner body surface 114A, a second inner body surface 114B, a third inner body surface 114C, a fourth inner body surface 114D and a fifth inner body surface ( 114E).
- the first inner body face 114A, the second inner body face 114B and the third inner body face 114C may be substantially parallel to the side body face 111B.
- the first inner body surface 114A, the second inner body surface 114B and the third inner body surface 114C may be sequentially positioned in a direction toward the second opening 116 .
- the first inner body surface 114A, the second inner body surface 114B, the third inner body surface 114C, the fourth inner body surface 114D, and the fifth inner body surface 114E are integrally and seamlessly formed. It can be.
- a first step S1 may be formed between the first inner body surface 114A and the second inner body surface 114B.
- the second inner body surface 114B may be formed to be recessed from the first inner body surface 114A.
- the first inner body surface 114A may protrude from the second inner body surface 114B.
- the second inner body surface 114B may be formed to protrude from the first inner body surface 114A.
- the first inner body surface 114A and the second inner body surface 114B may be integrally and seamlessly formed substantially without a first step S1.
- a second step S2 may be formed between the second inner body surface 114B and the third inner body surface 114C.
- the third inner body surface 114C may be recessed from the second inner body surface 114B.
- the second inner body surface 114B may protrude from the third inner body surface 114C.
- the third inner body surface 114C may be formed to protrude from the second inner body surface 114B.
- the second inner body surface 114B and the third inner body surface 114C may be integrally and seamlessly formed substantially without the second step S2.
- the fourth inner body face 114D may be substantially parallel to the second front body face 111A-2.
- the fourth inner body surface 114D may be inclined with respect to the first inner body surface 114A and the fifth inner body surface 114E.
- the fifth inner body face 114E may be substantially parallel to the first anterior body face 111A-1.
- the fifth inner body surface 114E may be formed to be recessed with respect to the fourth inner body surface 114D.
- the piston body 110 may include a first engagement portion 117 .
- the first engaging portion 117 may be at least partially engaged with the second fixing portion 170 .
- the first engagement portion 117 may be formed on the side body surface 111B.
- the first engaging portion 117 may include a slot.
- the first engagement portion 117 may include a protrusion.
- the first external sealing part 121 and the second external sealing part 122 may seal between the piston body 110 and the container 1041 .
- the first outer sealing portion 121 and the second outer sealing portion 122 may each have an annular shape.
- the first outer sealing part 121 may be positioned in the first outer recess 112A, and the second outer sealing part 122 may be positioned in the second outer recess 112B.
- the valve 130 may allow or block the flow of at least one material between the space in front of the piston body 110 and the space behind the piston body 110 .
- the valve 130 moves relative to the piston body 110 in a direction away from the axis of rotation X, in a centrifugal environment, in the space in front of the piston body 110 and behind the piston body 110. It is possible to block the fluid passage formed between the spaces of the.
- the valve 130 uses an environment in which centrifugal force does not act on the valve 130 or a force other than the centrifugal force acting on the valve 130 (eg, gravity, frictional force, plunger 107 of FIG. 12).
- the weight of the valve 130 may be substantially equal to or greater than the weight of the piston body 110 .
- the valve 130 includes a first valve body portion 131 substantially forming a front portion of the valve 130 and a second valve body portion 132 substantially forming a rear portion of the valve 130. can do.
- the first valve body portion 131 may include a first outer valve surface 133A, a second outer valve surface 133B, a third outer valve surface 133C, and a fourth outer valve surface 133D.
- the second valve body portion 132 may include a fifth outer valve face 134A and a sixth outer valve face 134B.
- 134B may form an outer valve face 133 of valve 130 .
- the first outer valve face 133A may form substantially at least a portion of the front portion of the first valve body portion 131 .
- the first outer valve face 133A may substantially face the fourth inner body face 114D.
- the first outer valve face 133A may be at least partially positioned in a recess on the fourth inner body face 114D, for example formed to be recessed in the fifth inner body face 114E.
- the second outer valve face 133B may form substantially at least a portion of the front portion of the first valve body portion 131 .
- the second outer valve surface 133B may be inclined with respect to the first outer valve surface 133A and the third outer valve surface 133C.
- the second outer valve face 133B may substantially face the fifth inner body face 114E.
- the third outer valve face 133C may substantially form a side portion of the first valve body portion 131 .
- the third outer valve face 133C may substantially face the first inner body face 114A, the second inner body face 114B and/or the third inner body face 114C.
- the fourth outer valve face 133D may substantially form at least a portion of the rear portion of the first valve body portion 131 .
- the fourth outer valve face 133D may be formed between the third outer valve face 133C and the fifth outer valve face 134A.
- the fifth outer valve face 134A may substantially form a side portion of the second valve body portion 132 .
- the fifth outer valve face 134A may substantially face the first inner body face 114A, the second inner body face 114B and/or the third inner body face 114C.
- the sixth outer valve face 134B may form substantially a rearward portion of the second valve body portion 132 .
- the first outer valve face 133A, the fourth outer valve face 133D and the sixth outer valve face 134B may be substantially parallel to each other.
- the third outer valve face 133C and the fifth outer valve face 134A may be substantially parallel to each other.
- the first outer valve surface 133A and the second outer valve surface 133B may be integrally and seamlessly formed.
- the second outer valve face 133B and the third outer valve face 133C may be formed discontinuously with respect to each other.
- the third outer valve surface 133C and the fourth outer valve surface 133D may be integrally and seamlessly formed.
- the fourth outer valve face 133D, the fifth outer valve face 134A, and the sixth outer valve face 134B may be integrally and seamlessly formed.
- the first outer valve face 133A, the second outer valve face 133B, the third outer valve face 133C, the fourth outer valve face 133D, the fifth outer valve face 134A, and The sixth outer valve face 134B may be integrally and seamlessly formed.
- the valve 130 may include a recess portion 135 formed in the first valve body portion 131 .
- the recess portion 135 may be formed, for example, between the second outer valve face 133B and the third outer valve face 133C.
- the first inner seal 140 may be configured to seal between the piston body 110 and the first valve body portion 131 .
- the first inner seal 140 may be located at least partially in the recessed portion 135 .
- the first inner seal 140 may be formed, for example, between the fourth inner body portion 114E and the second outer valve face 133B, or between the fourth inner body portion 114E and the third outer valve face 133C. ) can be sealed.
- the first inner seal 140 may be substantially annular.
- the valve 130 moves relative to the piston body 110 in a direction away from the axis of rotation X under the centrifugal force in the centrifugal separation environment, and the first outer valve face 133A and the second outer valve face 133B are respectively When the fourth inner body face 114D and the fifth inner body face 114E are substantially facing each other (eg, see FIG. 9 ), the first inner seal 140 is formed by the second outer valve face 133B and the second outer valve face 133B. 5 Space the inner body surfaces 114E apart from each other so that a gap (eg, gap G in FIG. 9) substantially exists between the second outer valve surface 133B and the fifth inner body surface 114E. While doing so, it may be configured to seal between the piston body 110 and the valve 130.
- a gap eg, gap G in FIG. 9
- the first inner seal 140 is, for example, in a structure without the first inner seal 140, the inner body surface 114 (eg, the fifth inner body surface 114E) of the piston body 110 ) and/or to substantially prevent or reduce leaks that may occur when the outer valve faces of valve 130 (eg, second outer valve face 133B) do not substantially contact each other due to manufacturing tolerances.
- the second inner seal 150 may be configured to seal between the piston body 110 and the plunger (eg, the plunger 107 of FIG. 12 ).
- the second inner sealing portion 150 may contact at least a portion of the outer surface of the plunger (eg, the plunger 107 of FIG. 12 ).
- the second inner seal 150 may be located on the second inner body surface 114B.
- the second inner seal 150 may be substantially annular.
- the first fixing part 160 may fix the second inner sealing part 150 to the second inner body surface 114B.
- the first fixing part 160 may be located on the second inner body surface 114B.
- the first fixing part 160 has a structure in which the third inner body surface 114C is formed to protrude from the second inner body surface 114B, and the second inner sealing part 150 has a second step.
- the second inner sealing part 150 may be fixed to the second inner body surface 114B so as to be supported against (S2).
- the first fixing part 160 may limit the movement of the valve 130 .
- an environment in which centrifugal force does not act on the valve 130 or a centrifugal force acting on the valve 130 does not block the fluid passage using other forces (eg, gravity, frictional force, plunger 107 of FIG. 12).
- valve 130 In an environment less than the pressure in the space in front of the piston 106), the valve 130 can move toward the second opening 116 relative to the piston body 110, and the first fixing part 160 is the valve ( 130) can be prevented from coming off of the valve 130 from the hollow portion 113 so that it is positioned within the hollow portion 113.
- the first fixing part 160 may include a first base part 161 and a protruding part 162 protruding from the first base part 161 .
- the first base portion 161 can be positioned on the second inner body surface 114B.
- the first base part 161 may be supported with respect to the first step S1.
- the first base part 161 may support the first part 151 (eg, the left part in FIG. 5 ) of the second inner sealing part 150 .
- the protruding portion 162 may be configured to contact a portion of the first valve body portion 131 (eg, the fourth outer valve surface 133D).
- the first base part 161 and the protruding part 162 may form a substantially annular structure of the first fixing part 160 .
- the protruding portion 162 may protrude from the inner circumferential surface of the first base portion 161 .
- the protruding portion 162 may be formed at least partially along the circumferential direction of the inner circumferential surface of the first base portion 161 .
- the protruding portion 162 may form an outlet opening 163 while extending along the inner circumferential surface of the first base portion 161 .
- the inflow and outflow opening 163 is, for example, when one surface of the valve 130 (eg, the sixth outer valve surface 134B) contacts the protruding portion 162, the inflow and outflow of the material through the inflow and outflow opening 163 can allow
- the second fixing part 170 may fix the second inner sealing part 150 to the second inner body surface 114B.
- the second fixing part 170 may be located on the third inner body surface 114C.
- the second fixing part 170 may fix the second inner sealing part 150 to the second inner body surface 114B so that the second inner sealing part 150 is supported by the first fixing part 160.
- the second fixing part 170 attaches the second inner sealing part 150 to the second inner body surface 114B so that the second inner sealing part 150 is supported by the first step S1. can also be fixed to
- the second fixing part 170 may cooperate with the first fixing part 160 to fix the second inner sealing part 150 .
- the second fixing part 170 may include a second base part 171 positioned on the third inner body surface 114C.
- the second base part 171 may support the second part 152 (eg, the right part in FIG. 5 ) of the second inner sealing part 150 .
- the second base part 171 may form a substantially annular structure of the second fixing part 170 .
- the second fixing part 170 may include a first fastening part 172 configured to be fastened with an external fastening means (eg, the plunger 107 of FIG. 12 ).
- the first fastening part 172 may be at least partially formed on the inner surface of the second base part 171 .
- the first fastening part 172 may include, for example, a screw thread.
- the second fixing part 170 may include a second engaging part 173 .
- the second engagement portion 173 may be engaged with at least a portion of the piston body 110 (eg, the first engagement portion 117).
- the second engagement portion 173 may be at least partially formed on an outer surface of the second base portion 171 .
- the second engagement portion 173 may include, for example, a protrusion.
- FIG. 9 is a cross-sectional view of a piston for centrifugal separation in a first state according to an embodiment.
- 10 is a cross-sectional view of a piston for centrifugal separation in a second state according to an embodiment.
- 11 is a cross-sectional view of a piston for centrifugal separation in a third state according to an embodiment.
- the centrifugal piston 106 blocks communication between the first passage P1 and the second passage P2 from the first state (eg, FIG. 9) to the first passage ( It is possible to transition to a second state (eg, FIG. 10 ) allowing communication between P1) and the second passage P2.
- the centrifugal piston 106 may transition from the first state (eg, via the second state) to a third state (eg, FIG. 11 ) that restricts the movement of the valve 130 .
- the centrifugal piston 106 may transition from the third state to the second state.
- the centrifugal piston 106 may transition from the second state to the first state.
- the centrifugal piston 106 may transition from the third state to the first state.
- the centrifugal force acting on the valve 130 having a first weight is greater than the centrifugal force acting on the piston body 110 having a second weight less than the first weight.
- the centrifugal force acting on the valve 130 moves the piston 106 without blocking the fluid passage using other forces acting on the valve 130 (e.g. gravity, frictional force, plunger 107 in FIG. 12).
- the valve 130 may move in a direction away from the second opening 116 relative to the piston body 110 (eg, forward of the piston body 110).
- the first inner seal 140 installed in the valve 130 contacts the inner body surface 114 of the piston body 110, and the second passage P2 between the piston body 110 and the valve 130 ( Example: In FIG. 10 , the first sub passage P21 between the fifth inner body surface 114E and the second outer valve surface 133B) may be blocked.
- the first inner seal part 140 includes at least a portion (eg, a fifth inner body surface 114E) of the inner body surface 114 of the piston body 110 and an outer valve surface 133 of the valve 130. At least a part (eg, the second external valve surface 133B) may be spaced apart from each other to form a gap G therebetween.
- the valve 130 In the second state of the piston 106, in a centrifugal environment or in an environment where no centrifugal force acts, the valve 130 is directed relative to the piston body 110 toward the second opening 116 (e.g., to the rear of the piston body 110).
- the first inner seal 140 installed in the valve 130 releases contact with the inner body surface 114 of the piston body 110, and the second passage P2 between the piston body 110 and the valve 130 (eg, the first sub passage P21 between the fifth inner body surface 114E and the second outer valve surface 133B in FIG. 10) may be opened.
- At least one substance in front of the piston body 110 enters the first passage P1 through the first opening (eg, the first opening 115 in FIG. 3), and enters the first passage P1 and the second passage P1. It flows through two passages P2 (eg, the first sub passage P21 and the second sub passage P22), and can escape to the rear of the piston body 110 through the second opening 116.
- the centrifugal piston 106 blocks the passages P1 and P2 in a first state (eg, FIG. 9 ) and in a third state where the movement of the valve 130 is restricted (eg, FIG. 11 ). ), or between the second state of opening the passages P1 and P2 (eg, FIG. 10) and the third state of restricting the movement of the valve 130 (eg, FIG. 11).
- valve 130 In the second state of the piston 106, in a centrifugal environment or in an environment where no centrifugal force acts, the valve 130 is directed relative to the piston body 110 toward the second opening 116 (e.g., to the rear of the piston body 110).
- first fixing part 160 located on the inner body surface 114 of the piston body 110 (e.g., the second inner body surface 114B in FIG. 6). Movement of the valve 130 may be limited. For example, at least a portion of the first valve body portion 131 among the first valve body portion 131 and the second valve body portion 132 (eg, the fourth outer valve surface 133D) is formed on the first base It may contact at least a portion of portion 161 or at least a portion of protruding portion 162 .
- the first fixing part 160 is a second inner body surface 114 (eg, the second inner body surface 114B and the third inner body surface 114C) respectively located on the inner body surface 114 of the piston body 110. It may be supported by the sealing part 150 and the second fixing part 170 .
- FIG. 12 is a cross-sectional view of a piston and plunger for centrifugal separation according to an embodiment.
- the centrifugal separation device 100 includes a piston body 110, a first outer seal 121, a second outer seal 122, a valve 130, and a first inner seal. It may include a piston 106 for centrifugal separation including a part 140, a second inner sealing part 150, a first fixing part 160 and a second fixing part 170, and a plunger 107. .
- the second fixing part 170 may include a second base part 171 and a first fastening part 172 .
- Plunger 107 may be configured to engage piston 106 .
- the plunger 107 engages with the piston 106 and then pressurizes a substance (eg, blood, medicine, and water) located in front of the piston 106 in the container 1041 to discharge the connector 1042. And through the injection passage 1042B, the substance may be discharged from the container 1041 or brought into the container 1041.
- a substance eg, blood, medicine, and water
- the plunger 107 includes a push rod 1071, a handle 1072 formed on one part of the push rod 1071 (eg, the end face on the right side in FIG. 12), and another part of the push rod 1071 (eg, FIG.
- the second fastening part 1073 formed on the outer surface of the left side at 12) and configured to be fastened with the first fastening part 172, and extending in the radial direction of the push rod 1071 and part of the piston body 110 (eg : A seat 1074 facing the rear body surface 111C) may be included.
- One fastening part of the first fastening part 1072 and the second fastening part 1073 may include a male thread, and the other fastening part may include a female thread.
- FIGS. 13 to 15 are diagrams illustrating an exemplary operation of a centrifugal separator according to an embodiment.
- biological tissue (BM) obtained from the human body is introduced through the front of the container 1041 (eg, the end portion where the connector 1042 is located), and a piston (BM) is placed at the rear of the biological tissue (BM). 106) may be located.
- the discharge and injection passage 1042B of the connector 1042 may be closed so that the biological tissue BM cannot escape to the outside of the container 1041 .
- the syringe 104 and piston 106 can rotate about an axis of rotation (X).
- the biological tissue (BM) in front of the piston 106 may be separated into a plurality of layers of materials constituting the biological tissue (BM) according to the specific gravity of the materials.
- the biological tissue BM when viewed in a direction from the connector 1042 away from the rotational axis X toward the piston 106, sequentially includes at least one first material having a first specific gravity (eg : A first layer (RL) composed of blood, drug solution, and water), and at least one second substance having a second specific gravity smaller than the first specific gravity (e.g., adipose tissue from which the first and third substances have been removed) It may be separated into a second layer FL and a third layer OL composed of at least one third material (eg, air or oil) having a third specific gravity smaller than the second specific gravity.
- a first specific gravity eg : A first layer (RL) composed of blood, drug solution, and water
- second substance having a second specific gravity smaller than the first specific gravity e.g., adipose
- the piston 106 and the plunger 107 may be coupled to each other.
- the first fastening part 172 of the second fixing part 170 located on the inner body surface (eg, the third inner body surface 114C of FIG. 6) of the piston body 110 and the plunger The second fastening parts 1073 formed on the push rod 1071 of 107 may be fastened to each other.
- the plunger 107 and the second fastening part 1073 are engaged and the piston 106 is pushed by the plunger 107, at least one material (eg, the first material) in front of the piston 106 is connected to the connector. It is discharged to the outside of the container 1041 through 1042, and a layer of the material (eg, the first layer RL in FIG. 13) may be removed from the container 1041.
- a third layer OL of a third substance located relatively close to the piston 106 may, in various ways, move the piston 106 It can be discharged to the rear.
- a second layer (FL) of a second material eg, adipose tissue or fat mass from which the first material and the third material are removed
- a third layer (OL) of the third material are formed in front of the piston 106.
- the inner body surface of the piston body (eg, the piston body 110 of FIGS. 2 to 7, 8A and 8B) in the second state (eg, FIG. 10) and the third state (eg, FIG. 11) Example: between a fifth inner body face 114E) and an outer valve face (eg, second outer valve face 133B) of a valve (eg, valve 130 of FIGS. 2-7 , 8A and 8B).
- the size of the flow space (eg, gap) of the first sub-passage (P21) of the piston 106 is smaller than the size of the second material (eg, adipose tissue) of the second layer (FL) in the front space of the piston (106).
- the size of the gap between the inner body face of the body (eg, the fifth inner body face 114E) and the outer valve face of the valve (eg, the second outer valve face 133B) ranges from about 5 ⁇ m to about 150 ⁇ m.
- the first fixing part eg, the first fixing part 160 of FIGS. 2 to 7, 8A and 8B
- the first fixing part 160 of FIGS. 2 to 7, 8A and 8B may be a valve (eg, FIGS. 2 to 7, The movement of the valve 130 of FIGS. 8A and 8B may be restricted.
- the second material of the second layer FL is the piston 106 ) Can be located in front of the piston 106 without exiting to the rear of the piston 106 through the passage formed in (eg, the first passage P1 and the second passage P2 of FIG. 10).
- the size range of the gap can be substantially maintained by adjusting the mass of the valve 130 and/or the centrifugal force applied to the piston 106.
- a pneumatic device is coupled to the rear (eg, flange 1043) of the vessel 1041, and a passage formed in the piston 106 (eg, the first passage P1 and the second passage P2 in FIG. 10) ) is opened, the third material of the third layer OL in front of the piston 106 may be discharged to the rear of the piston 106 by using a pneumatic device.
- pushing the piston 106 forward of the syringe 104 without engaging the plunger 107 and/or other manipulation tool to the piston 106 increases the pressure in front of the piston 106, causing the piston ( 106)
- the third material of the third layer OL in the front may be discharged to the rear of the piston 106. After the third material is removed and the piston 106 is removed from the syringe 104, the second material of the second layer FL may be obtained.
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Abstract
Description
Claims (12)
- 원심분리용 피스톤에 있어서,외부 바디 면 및 내부 바디 면을 갖는 피스톤 바디로서, 상기 피스톤 바디는 상기 내부 바디 면에 의해 규정된 중공 부분을 포함하고, 상기 피스톤 바디는, 상기 외부 바디 면에 위치된 제 1 개구, 상기 외부 바디 면의 적어도 일부에 의해 규정되고 상기 내부 바디 면 및 상기 중공 부분에 연결된 제 2 개구, 및 상기 제 1 개구 및 상기 중공 부분을 연결하는 제 1 통로를 포함하는, 상기 피스톤 바디;상기 제 1 통로를 개방 또는 차단하도록 구성되고 상기 내부 바디 면으로부터 이격되게 상기 중공 부분에 위치된 밸브로서, 상기 밸브는, 상기 내부 바디 면 및 외부 밸브 면 사이에 상기 제 1 통로 및 상기 제 2 개구에 유체 연결된 제 2 통로를 규정하는 상기 외부 밸브 면을 갖고, 상기 밸브는 상기 외부 밸브 면에 형성된 리세스 부분을 포함하는, 상기 밸브; 및상기 리세스 부분에 위치되고, 상기 내부 바디 면에 접촉하고 상기 외부 밸브 면을 상기 내부 바디 면으로부터 이격시키도록 구성되고, 원심분리 시 상기 내부 바디 면 및 상기 외부 밸브 면 사이를 밀봉하도록 구성된 제 1 내부 밀봉부;를 포함하는 피스톤.
- 제 1 항에 있어서,상기 내부 바디 면 상에 위치되고 상기 제 2 개구를 향하는 상기 밸브의 이동을 제한하도록 구성된 제 1 고정부를 더 포함하는 피스톤.
- 제 2 항에 있어서,상기 밸브는,상기 리세스 부분을 포함하는 제 1 밸브 바디 부분; 및상기 제 1 밸브 바디 부분에 연결된 제 2 밸브 바디 부분;을 포함하고,상기 제 1 고정부는,상기 내부 바디 면 상에 위치된 베이스 부분; 및상기 베이스 부분으로부터 돌출하고 상기 제 1 밸브 바디 부분과 접촉하도록 구성된 돌출 부분;을 포함하는 피스톤.
- 제 3 항에 있어서,상기 내부 바디 면 및 상기 외부 밸브 면 사이의 간격의 크기가 5 ㎛ 내지 150 ㎛에 있도록 상기 제 1 밸브 바디 부분 및 상기 돌출 부분이 접촉하는 피스톤.
- 제 3 항에 있어서,상기 제 2 통로는,상기 내부 바디 면 및 상기 제 1 밸브 바디 부분 사이에 규정된 제 1 서브 통로; 및상기 내부 바디 면 및 상기 제 2 밸브 바디 부분 사이에 규정된 제 2 서브 통로;를 포함하고,상기 밸브는 상기 제 1 고정부와 접촉하고 상기 제 1 서브 통로로부터 상기 제 2 서브 통로로 적어도 하나의 물질의 이동을 억제 또는 지연하도록 구성된 피스톤.
- 제 2 항에 있어서,상기 내부 바디 면은, 제 1 내부 바디 면 및 상기 제 1 내부 바디 면으로부터 리세스되고 상기 제 1 내부 바디 면과 제 1 단차를 형성하는 제 2 내부 바디 면을 포함하고, 상기 제 1 고정부는 상기 제 2 내부 바디 면 상에 위치되는 피스톤.
- 제 1 항에 있어서,상기 내부 바디 면 상에 위치되고, 제 1 부분 및 상기 제 1 부분에 반대되는 제 2 부분을 포함하는 제 2 내부 밀봉부; 및상기 내부 바디 면 상에 위치되고 상기 제 2 내부 밀봉부의 제 1 부분을 지지하며 상기 제 2 내부 밀봉부를 상기 내부 바디 면에 고정시키도록 구성된 제 1 고정부;를 더 포함하는 피스톤.
- 제 7 항에 있어서,상기 내부 바디 면 상에 위치되고 상기 제 2 내부 밀봉부의 제 2 부분을 지지하며 상기 제 2 내부 밀봉부를 상기 내부 바디 면에 고정시키도록 구성된 제 2 고정부를 더 포함하는 피스톤.
- 제 8 항에 있어서,상기 피스톤 바디는 제 1 맞물림부를 포함하고,상기 제 2 고정부는 상기 제 1 맞물림부와 맞물리도록 구성된 제 2 맞물림부를 포함하는 피스톤.
- 제 8 항에 있어서,상기 제 2 고정부는 체결부를 포함하는 피스톤.
- 회전축을 갖는 원심분리 장치에 있어서,상기 회전축에 대해 회전하도록 구성된 시린지로서, 상기 시린지는 생체조직을 보유할 수 있는 용기를 포함하는 시린지; 및상기 용기의 내부에 이동 가능하게 위치된 원심분리용 피스톤;을 포함하고,상기 피스톤은,외부 바디 면 및 내부 바디 면을 갖는 피스톤 바디로서, 상기 피스톤 바디는 상기 내부 바디 면에 의해 규정된 중공 부분을 포함하고, 상기 피스톤 바디는, 상기 외부 바디 면에 위치된 제 1 개구, 상기 외부 바디 면의 적어도 일부에 의해 규정되고 상기 내부 바디 면 및 상기 중공 부분에 연결된 제 2 개구, 및 상기 제 1 개구 및 상기 중공 부분을 연결하는 제 1 통로를 포함하는, 상기 피스톤 바디;상기 제 1 통로를 개방 또는 차단하도록 구성되고 상기 내부 바디 면으로부터 이격되게 상기 중공 부분에 위치된 밸브로서, 상기 밸브는, 상기 내부 바디 면 및 외부 밸브 면 사이에 상기 제 1 통로 및 상기 제 2 개구에 유체 연결된 제 2 통로를 규정하는 상기 외부 밸브 면을 갖고, 상기 밸브는 상기 외부 밸브 면에 형성된 리세스 부분을 포함하는, 상기 밸브; 및상기 리세스 부분에 위치되고, 상기 내부 바디 면에 접촉하고 상기 외부 밸브 면을 상기 내부 바디 면으로부터 이격시키도록 구성되고, 원심분리시 상기 내부 바디 면 및 상기 외부 밸브 면 사이를 밀봉하도록 구성된 제 1 내부 밀봉부;를 포함하는 원심분리 장치.
- 제 11 항에 있어서,상기 피스톤은,상기 내부 바디 면 상에 위치되고, 제 1 부분 및 상기 제 1 부분에 반대되는 제 2 부분을 포함하는 제 2 내부 밀봉부;상기 내부 바디 면 상에 위치되고 상기 제 2 내부 밀봉부의 제 1 부분을 지지하며 상기 제 2 내부 밀봉부를 상기 내부 바디 면에 고정시키도록 구성된 제 1 고정부;상기 내부 바디 면 상에 위치되고 상기 제 2 내부 밀봉부의 제 2 부분을 지지하며 상기 제 2 내부 밀봉부를 상기 내부 바디 면에 고정시키도록 구성된 제 2 고정부; 및상기 제 2 고정부와 체결되도록 구성된 플런저;를 더 포함하고,상기 제 2 내부 밀봉부는 상기 플런저와 접촉하며 상기 내부 바디 면 및 상기 플런저 사이를 밀봉하도록 구성된 원심분리 장치.
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KR20130088113A (ko) * | 2013-07-29 | 2013-08-07 | 이희영 | 비중 구분용 주사기 및 지방 분리 방법 |
KR20150120797A (ko) * | 2014-04-18 | 2015-10-28 | 메디칸(주) | 피스톤 |
KR20150122102A (ko) | 2015-10-12 | 2015-10-30 | (주)아모레퍼시픽 | 유색콩 추출물을 함유하는 조성물 |
KR20190026585A (ko) * | 2017-09-05 | 2019-03-13 | 이준석 | 원심분리용 피스톤 및 이를 포함하는 원심분리 장치 |
KR20190092105A (ko) * | 2018-01-30 | 2019-08-07 | 이준석 | 원심분리용 피스톤 |
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2021
- 2021-08-26 KR KR1020210112893A patent/KR102446918B1/ko active IP Right Grant
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- 2022-08-18 CN CN202280058091.7A patent/CN117897181A/zh active Pending
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KR20050122102A (ko) * | 2004-06-23 | 2005-12-28 | 메디칸(주) | 지방 흡입 이식용 주사기의 피스턴 |
KR20130088113A (ko) * | 2013-07-29 | 2013-08-07 | 이희영 | 비중 구분용 주사기 및 지방 분리 방법 |
KR20150120797A (ko) * | 2014-04-18 | 2015-10-28 | 메디칸(주) | 피스톤 |
KR20150122102A (ko) | 2015-10-12 | 2015-10-30 | (주)아모레퍼시픽 | 유색콩 추출물을 함유하는 조성물 |
KR20190026585A (ko) * | 2017-09-05 | 2019-03-13 | 이준석 | 원심분리용 피스톤 및 이를 포함하는 원심분리 장치 |
KR20190092105A (ko) * | 2018-01-30 | 2019-08-07 | 이준석 | 원심분리용 피스톤 |
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CN117897181A (zh) | 2024-04-16 |
KR102446918B1 (ko) | 2022-09-26 |
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