WO2023026361A1 - Cloisons - Google Patents

Cloisons Download PDF

Info

Publication number
WO2023026361A1
WO2023026361A1 PCT/JP2021/030988 JP2021030988W WO2023026361A1 WO 2023026361 A1 WO2023026361 A1 WO 2023026361A1 JP 2021030988 W JP2021030988 W JP 2021030988W WO 2023026361 A1 WO2023026361 A1 WO 2023026361A1
Authority
WO
WIPO (PCT)
Prior art keywords
septa
cylindrical
container
main body
microplate
Prior art date
Application number
PCT/JP2021/030988
Other languages
English (en)
Japanese (ja)
Inventor
隆介 木村
基博 山崎
Original Assignee
株式会社日立ハイテク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社日立ハイテク filed Critical 株式会社日立ハイテク
Priority to PCT/JP2021/030988 priority Critical patent/WO2023026361A1/fr
Publication of WO2023026361A1 publication Critical patent/WO2023026361A1/fr

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations

Definitions

  • the present invention relates to a septa that seals containers such as wells of microplates and microtubes in a state in which thin tubes such as capillaries and needle nozzles can be inserted and extracted.
  • electrophoresis is used to analyze DNA, proteins, etc.
  • a capillary electrophoresis device having capillaries is widely used.
  • a capillary is a thin tube with a hollow structure, and an inner layer is formed of silica or the like to which charged functional groups are bonded. Liquid samples dispensed into microplates or microtubes are analyzed qualitatively or quantitatively in capillary electrophoresis devices.
  • the tip of the capillary When analyzing a liquid sample, the tip of the capillary is inserted into the well of the microplate or the liquid sample in the microtube.
  • a capillary is used in a state in which the inside is filled with a migration medium.
  • a voltage is applied across the capillary, an electroosmotic flow is formed within the capillary.
  • the components in the liquid sample are drawn into the capillary by electroosmotic flow, and are separated by their migration speed based on their charge and size while flowing through the capillary.
  • the separated components are optically detected by a downstream detector in the capillary.
  • capillary electrophoresis equipment is equipped with an autosampler that automatically performs analysis operations such as sampling.
  • the capillary is fixed in the device so that the tip opens downward.
  • a microplate or microtube containing the liquid sample is prepared on the moving stage.
  • the moving stage is provided so as to be able to move three-dimensionally with respect to the tip of the capillary.
  • a microplate or microtube containing a liquid sample is transported horizontally to below the tip of the capillary by the moving stage, and then vertically moved up and down with respect to the tip of the capillary.
  • the container rises from below with respect to the tip of the capillary that is open downward, the tip of the capillary is inserted into the well of the microplate or the microtube, and the liquid sample can be sucked.
  • the liquid sample placed in the container may evaporate or be exposed to suspended matter in the air after preparation or during automatic analysis. If the amount of the liquid sample is as small as several hundred ⁇ L to 1.5 mL, evaporation of the components will greatly affect the analysis results. In addition, contamination occurs when suspended matter in the air is mixed. To prevent such evaporation and contamination problems, containers containing liquid samples are fitted with septa.
  • a septum has the function of sealing a container containing a sample, etc., in such a way that a thin tube such as a capillary can be inserted and extracted.
  • a septa having a structure for sealing a plurality of arranged containers is used in a microplate in which a plurality of wells are formed and a multiple microtube in which a plurality of microtubes are connected.
  • a septa is provided as a sheet-like cover with an elastic elastomer.
  • a general septa is composed of a sheet-like main body indicated by reference numeral 10, a hole indicated by reference numeral 20, and a bottomed tubular shape indicated by reference numeral 30. and The tubular portion is provided so as to elastically fit inside openings of a plurality of arranged containers, such as a plurality of wells provided on a microplate and a plurality of microtubes connected to each other.
  • the hole part and the cylindrical part form a penetrating structure that allows a thin tube such as a capillary to pass through toward the inside of the container.
  • a slit is provided in the bottom of the tubular portion. The slit is provided so that when a thin tube such as a capillary is inserted, it is elastically deformed and opened by pressure from the thin tube, and closed by an elastic restoring force when the thin tube is pulled out. The elastically opened and closed slit allows the narrow tube to be inserted into the container while suppressing the opening of the container.
  • a divided structure container in which a plurality of container parts are integrally formed and provided so as to be divided into individual container parts.
  • a container having a split structure is formed with a mechanically weak region so that it can be easily split by hand.
  • Patent Literature 1 describes a multi-well sample testing device that includes a sample tray in which a plurality of wells are formed. Connecting members are integrally formed between portions of the sample tray. The connecting member is supposed to be breakable or destroyable. The parts of the sample tray can be separated from each other by breaking or breaking the connection member (see paragraphs 0085 to 0089).
  • Patent Document 2 describes an easily separable container that can be separated when used.
  • the easily separable container has a container material in which a separating portion is formed at a position for separating the container.
  • the separation is formed as a half cut between the containers.
  • the separation section includes a cut section formed by completely cutting only the container material in the thickness direction at a position that will be the edge when separating the container (see paragraphs 0015, 0031, 0038, etc.).
  • Patent Document 3 describes a connected container in which a plurality of unit containers having storage portions for storing contents are connected via breakable weakened portions.
  • the connecting container is configured such that the unit container can be cut off from the connecting container by breaking the weakened portion (see paragraphs 0007, 0009, 0015, etc.).
  • a septa for a microplate has holes and cylindrical parts arranged in a matrix on a sheet-like main body.
  • the hole portion and the tubular portion form a structural unit that seals a well provided on the microplate in such a manner that a thin tube such as a capillary can be inserted and removed.
  • a single sheet-like septa is attached to the upper surface of the microplate.
  • a single sheet-like septa covers all the wells provided on the microplate.
  • a well that does not contain a sample or the like does not need to be sealed with a septa, but a conventional septa has a structure that uniformly covers all the wells. As a result, conventional septa are functionally wasteful and cost unnecessary. A conventional septa can be cut with scissors, a cutter, or the like, but this is troublesome for the user.
  • Patent Documents 1 to 3 disclose containers with split structures.
  • a septa with a split structure is not known.
  • Microplates to which septa are attached may have any number of wells, from several to several tens, for various analyzes, tests, experiments, and the like.
  • a septa is provided as a highly flexible sheet. It is desirable to have a divided structure that can easily secure the connection between the two parts by resin molding.
  • an object of the present invention is to provide a septa that can be used individually by dividing a plurality of structural units that seal a thin tube such as a capillary in an insertable and removable state.
  • the septa according to the present invention comprises a plurality of cylindrical portions that can be fitted inside openings of a plurality of arranged containers, and slits formed in the bottoms of the respective cylindrical portions.
  • a plurality of structural units for sealing thin tubes such as capillaries in an insertable and removable state can be divided and used individually.
  • FIG. 1 is a perspective view showing a septa and a microplate according to an embodiment of the invention
  • FIG. FIG. 4 is a cross-sectional view showing a state in which the septa according to the embodiment of the present invention are attached to the microplate
  • FIG. 4 is a top view of a septa showing a structural example of a parting line
  • FIG. 4 is a cross-sectional view of a septa showing a structural example of a parting line
  • FIG. 4 is a cross-sectional view of a septa showing a structural example of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of a parting line
  • FIG. 4 is a cross-sectional view of a septa showing a structural example of a parting line
  • FIG. 4 is a cross-sectional view of a septa showing a structural example of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of a parting line
  • FIG. 4 is a cross-sectional view of a septa showing a structural example of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of an end portion of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of an end portion of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of an end portion of a parting line
  • FIG. 4 is a top view of a septa showing a structural example of an end portion of a parting line;
  • FIG. 4 is a top view of the septa showing a structural example of the main body and the parting line;
  • 1 is a perspective view showing a septa and multiple microtubes according to an embodiment of the present invention;
  • FIG. 1 is a perspective view showing a septa and a microplate according to an embodiment of the invention.
  • FIG. 2 is a cross-sectional view showing a state in which the septa according to the embodiment of the present invention are attached to the microplate.
  • 1 and 2 show a septa 1 for a microplate and a microplate 200 to which the septa 1 is attached as an example of the septa according to this embodiment.
  • the microplate 200 is used as a container for analyses, inspections, experiments, and the like.
  • the microplate 200 is provided in a substantially rectangular plate shape with resin or the like having high rigidity.
  • resin or the like having high rigidity.
  • polystyrene or polyolefin such as polypropylene is used.
  • the microplate 200 is generally made of resin, but may be made of glass or the like.
  • a concave portion 210 is formed on the top surface of the microplate 200 .
  • the concave portion 210 has a rectangular shape in a plan view of the microplate 200 and is formed over substantially the entire upper surface.
  • the recess 210 is surrounded by a thin plate-like outer frame 220 .
  • the upper surface side of the microplate 200 forms a dish-shaped concave portion 210 by thinning with a substantially uniform thickness while leaving the outer frame 220 around.
  • a plurality of wells 230 are formed in the recess 210 of the microplate 200 .
  • the well 230 has a circular shape in a plan view of the microplate 200 and is provided as a tapered, substantially cylindrical depression.
  • Well 230 opens to the bottom surface of recess 210 .
  • the wells 230 are arranged in a matrix on the bottom surface of the recess 210 at intervals.
  • the well 230 is composed of a cylindrical upper portion 231 and a bottomed cylindrical lower portion 232 tapering downward in diameter.
  • a cylindrical space is formed inside the upper portion 231 .
  • the space inside the well 230 functions as a container, and is filled with a desired liquid or the like.
  • liquids include liquid samples, solutions in which solid samples are dissolved, dispersions in which solid samples such as powders are dispersed, buffers, standard samples, and the like.
  • the liquid itself to be analyzed dispensed into the well 230, the solution or dispersion liquid containing the component to be analyzed, the liquid reactant, the liquid culture, etc., can be used for various analyses, inspections, experiments, and the like. be done.
  • the microplate 200 is a 96-well microplate in which a total of 96 wells 230 are formed in 8 rows ⁇ 12 columns.
  • the well 230 has a capacity of 100 to 400 ⁇ L, an inner diameter of 5 to 8 mm, and a depth of 6 to 20 mm.
  • the capacity, inner diameter, outer diameter, depth, mutual spacing, etc. of the wells 230 differ according to the number of wells in the microplate 200, the arrangement of the wells, and the like.
  • the recess 210 is provided on the upper surface side of the microplate 200, and the well 230 is provided as a tapered, substantially cylindrical depression. is not particularly limited as long as it corresponds to the septa 1 .
  • well 230 may be provided as a substantially cylindrical depression.
  • the bottom of well 230 may be flat-bottomed, round-bottomed, U-bottomed, V-bottomed, or the like.
  • the microplate 200 is used as a container for setting samples in an automatic analyzer equipped with an autosampler.
  • automatic analysis devices include capillary electrophoresis devices, high performance liquid chromatography (HPLC) devices, biochemical analysis devices that perform other component analysis, reaction analysis, etc., chemical analysis devices, An optical analysis device and the like can be mentioned.
  • the autoanalyzer as a device that constitutes an autosampler, includes thin tubes for aspirating or discharging liquid into containers such as the wells 230 of the microplate 200, microtubes, and microvials, wells 230 of the microplate 200, microtubes, A moving stage or the like is provided for relatively moving the container such as a micro vial and the tip of the thin tube.
  • the tubule is inserted into the container like nozzle 400 shown in FIG. 4A.
  • a thin tube that aspirates or discharges liquid is installed in the automatic analyzer so that the tip opens downward.
  • the capillaries may have only the function of sucking liquid from containers such as the wells 230 of the microplate 200, microtubes, and microvials, or may only have the function of discharging liquid into these containers. , may have both functions.
  • capillary tubes include long and flexible capillaries used for separation operations such as electrophoresis, metal needles that have the function of aspirating or ejecting liquid, and capillaries that have the function of aspirating or ejecting liquid.
  • the moving stage may move containers such as the wells 230 of the microplate 200, microtubes, and microvials relative to the tips of the capillaries fixed in the device, or may move the containers fixed in the device. On the other hand, the tip of the capillary or the entire capillary may be relatively moved. Relative movement by the moving stage is performed in horizontal and vertical directions.
  • the septa 1 includes a main body portion 10 formed in a sheet shape, a plurality of holes 20 penetrating vertically through the main body portion 10, and a lower surface of the main body portion 10.
  • a bottomed tubular portion 30 formed so as to protrude downward from the periphery of each of the side holes 20 , and slits 40 formed in the bottom portions of the tubular portions 30 .
  • the septa 1 is provided in a split structure that can be split into split units constituted by one or more holes 20 and cylindrical parts 30 .
  • parting lines 100 are formed between the holes 20 .
  • the parting line 100 forms a structure that facilitates the separation of the divisional units constituted by the hole portion 20 and the tubular portion 30 .
  • the septa 1 is provided for a 96-well microplate.
  • a septa 1 for a 96-well microplate has a total of 96 holes 20 and tubular parts 30 in 8 rows ⁇ 12 columns at positions corresponding to wells 230 of a microplate 200 .
  • slits 40 are provided at the bottom of each cylindrical portion 30 .
  • the septa 1 have the function of sealing the wells 230 of the microplate 200 in such a manner that thin tubes 400 (see FIG. 4A) such as capillaries, needles and nozzles of the automatic analyzer can be inserted and extracted.
  • the septa 1 are attached to the upper surface of the microplate 200 as shown in FIG. 2 after the samples are placed in the wells 230 of the microplate 200 and before the microplate 200 is set in the automatic analyzer.
  • the body portion 10, the hole portion 20 and the cylindrical portion 30 of the septa 1 are integrally resin-molded with an elastic elastomer.
  • Materials for the septa 1 include silicone rubber, fluororubber, ethylene-propylene-diene rubber (EPDM), and the like.
  • the tubular portion 30 is provided with an elastic modulus to the extent that it is easily elastically deformed against pressure when it is inserted into the well 230 and pressure from a thin tube of an automatic analyzer.
  • Compression molding, transfer molding, or the like is used as a method of resin-molding the septa 1 .
  • Compression molding is a method in which a resin material is placed in a molding die and pressed under heat for molding.
  • Transfer molding is a method in which a heated resin material is injected into a molding die and pressurized to perform molding.
  • Compression molding is preferable as a resin molding method because the structure of the molding die is simple and productivity is high.
  • the body part 10 can be provided with a size that can be accommodated in the recess 210 of the microplate 200, as shown in FIGS.
  • the length and width of body portion 10 can be provided to be smaller than the length and width of recess 210 .
  • the thickness of the body portion 10 can be set equal to or smaller than the depth of the recess 210 .
  • the holes 20 and the cylindrical parts 30 are arranged in a matrix on the main body 10 at intervals so as to correspond to the wells 230 of the microplate 200 .
  • the hole portion 20 and the tubular portion 30 form a penetrating structure penetrating the septa 1 vertically.
  • the hole portion 20 has a circular shape in a plan view of the main body portion 10, and is provided as a substantially inverted truncated cone-shaped through hole that penetrates the main body portion 10 vertically.
  • One end of the hole portion 20 is open to the upper surface of the body portion 10 .
  • the other end of the hole portion 20 opens inside the cylindrical portion 30 on the lower surface side of the main body portion 10 .
  • the inner diameter of the hole 20 is equal to or smaller than the inner diameter of the well 230 .
  • the hole portion 20 may be provided as a substantially columnar through hole penetrating vertically through the main body portion 10 .
  • the cylindrical portion 30 has a circular shape in plan view of the body portion 10 and protrudes downward from the periphery of the hole portion 20 on the lower surface side of the body portion 10 in a cylindrical shape with a bottom.
  • the tubular portion 30 is arranged concentrically with the hole portion 20 .
  • the inner peripheral wall of the cylindrical portion 30 continues downward from the lower end of the inner peripheral wall of the hole portion 20 .
  • the hole portion 20 and the cylindrical portion 30 form a recess-like penetrating structure that vertically penetrates the septa 1 .
  • the tubular portion 30 is provided so as to be fitted inside the opening of the well 230 provided on the microplate 200 .
  • each tube 30 is inserted into each well 230 .
  • the outer diameter of the tubular portion 30 is equal to or slightly larger than the inner diameter of the well 230 .
  • the cylindrical portion 30 is provided so as to be easily elastically deformed by pressure from the inner wall of the well 230 and to be pressed against the inner wall of the well 230 by elastic restoring force.
  • the tubular portion 30 when the tubular portion 30 is inserted into the well 230, the tubular portion 30 is pressed from the inner wall of the opening of the well 230 toward the central axis of the tubular portion 30, and is slightly elastically deformed so as to be crushed in the radial direction. to fit into the opening of well 230 .
  • the tubular portion 30 After being inserted into the well 230 , the tubular portion 30 is pressed against the inner wall of the well 230 by an elastic restoring force, and frictional force is generated against the pulling force of the tubular portion 30 from the well 230 .
  • the septa 1 is detachably fixed to the microplate 200 .
  • a slit 40 is formed in the bottom of the cylindrical portion 30.
  • the slit 40 forms a through hole penetrating vertically through the bottom of the tubular portion 30 .
  • thin tubes such as the capillaries, needles, and nozzles 400 of the automatic analyzer are passed through the inside of the hole 20 and the tubular portion 30 and inserted into the slit 40 .
  • the septa 1 is in an inserted state in which fine tubes such as capillaries, needles, and nozzles 400 of an automatic analyzer are inserted through the cylindrical portion 30 into the wells 230 of the microplate 200, like conventional general septa. and an extracted state in which the tubules are extracted from the wells 230 of the microplate 200 to the outside of the tubular portion 30 .
  • a slit 40 formed in the bottom of the cylindrical portion 30 is structured to open and close by elastic deformation.
  • the relative movement between the predetermined wells 230 of the microplate 200 and the tips of the fine tubes is driven. First, horizontal relative motion is driven until a given well 230 is located below the tip of the capillary tube. A relative vertical movement is then driven.
  • the tip of the capillary In the extracted state, when the well 230 and the tip of the capillary tube are driven to move vertically relative to each other, the tip of the capillary penetrates the hole 20 and the tubular part 30 in the axial direction, It is inserted into well 230 through slit 40 .
  • the tip portion of the capillary tube moves out of the well 230 from the cylindrical portion 30 and the hole portion 20 . pulled out to the outside.
  • the septa 1 similarly to a conventional general septa, when the septa 1 shifts from the extracted state to the inserted state, the slit 40 is opened by elastic deformation of the cylindrical portion 30 due to the pressure from the thin tube, and the thin tube is inserted. are provided to allow the insertion of On the other hand, when transitioning from the inserted state to the extracted state or in the extracted state before insertion, the elastic force of the cylindrical portion 30 closes the slit 40 to seal the well 230 . .
  • the well 230 in which the sample is placed can be sealed by the slit 40 that opens and closes elastically in such a manner that the thin tube can be inserted and removed. Therefore, it is possible to suppress the evaporation of components from the well 230 and the entry of contaminants into the well 230 while permitting insertion of the capillary into the well 230 and removal of the capillary from the well 230 . Since the amount of sample placed in the well 230 is often small, if evaporation progresses before or during analysis, the tip of the capillary tube may be exposed to the gas phase, or the concentration of the sample may change. In addition, there is a risk of contamination due to contamination by suspended matter in the air. However, sealing the well 230 with the septa 1 enables accurate and stable analysis.
  • the holes 20 and the tubular portions 30 are each capable of inserting and removing a capillary, needle, nozzle 400 or the like of the automatic analyzer through wells 230 provided on the microplate 200. It forms a structural unit that seals in a state.
  • the septa 1 has a plurality of such structural units corresponding to the plurality of wells 230 provided on the microplate 200 .
  • a plurality of hole portions 20 and cylindrical portions 30 formed on the main body portion 10 are provided so as to be divisible from each other in division units constituted by one or more hole portions 20 and cylindrical portions 30 .
  • the septa 1 is provided so that a plurality of structural units formed by the hole portion 20 and the cylindrical portion 30 can be divided and used separately.
  • a parting line 100 is formed on the body portion 10 of the septa 1 .
  • the parting line 100 is formed between the dividing units composed of the hole portion 20 and the cylindrical portion 30 .
  • the dividing line 100 is provided as a mechanically fragile structure that easily breaks the main body 10 in order to facilitate the separation of the division units.
  • the division unit constituted by the hole portion 20 and the tubular portion 30 constitutes a part of the single sheet-like body portion 10 in which the hole portion 20 and the tubular portion 30 are formed.
  • the septa 1 shown in FIGS. 1 and 2 has a total of 96 holes 20 and cylindrical portions 30 in 8 rows and 12 columns, which can be divided into 4 rows and 12 columns and can be divided into each column. is provided.
  • the minimum division unit is 4 rows ⁇ 1 column, and has a total of 4 structural units that seal the wells 230 provided on the microplate 200 in such a manner that the fine tubes of the automatic analyzer can be inserted and removed. ing.
  • the division units constituted by the hole portion 20 and the tubular portion 30 can be separated at any section of the division line 100 formed on the main body portion 10 . From the entire septa 1, one minimum division unit can be separated, or a set of connected minimum division units can be separated. A portion configured by one or more division units can be separated from the entire septa 1 to cover a portion of the plurality of wells 230 provided on the microplate 200 .
  • the septa 1 is provided so that the hole portion 20 and the cylindrical portion 30 can be divided into 4 rows ⁇ 12 columns and can be divided into each column.
  • the number of holes 20 and cylindrical portions 30 that form the smallest division unit and the pattern of the division lines 100 that divide the division units are appropriately provided according to the number of wells of the microplate 200, the arrangement of the wells, and the like. be able to.
  • the minimum division unit is 4 rows ⁇ 2 columns, 4 rows ⁇ 3 columns, 4 rows ⁇ 4 columns, 8 rows ⁇ 1 column, 8 rows ⁇ 2 columns, 8 rows ⁇ 3 columns, 8 rows ⁇ 4 columns.
  • it may be 2 rows ⁇ 1 column, 2 rows ⁇ 2 columns, 2 rows ⁇ 3 columns, 2 rows ⁇ 4 columns, or the like.
  • the divided units may be configured with the same number of holes 20 and cylindrical parts 30 or different numbers of holes 20 and cylindrical parts 30 .
  • the split units constituted by the hole portion 20 and the cylindrical portion 30 are joined together with a tension that allows manual splitting. If both sides sandwiching the parting line 100 are joined with such tension, the split units can be easily separated by manual pulling or the like.
  • the tension as the maximum allowable load for joining the split units is, for example, 50 N or less, preferably set to 30 N or less, more preferably set to 10 N or less from the viewpoint of easy manual division.
  • the divided unit composed of the hole portion 20 and the cylindrical portion 30 constitutes a part of the single sheet-like main body portion 10 .
  • the dividing line 100 for facilitating the separation of the dividing units can be provided between the dividing units as an appropriate structure such as a perforation, groove, or notch.
  • FIG. 3 is a top view of a septa showing a structural example of a dividing line.
  • FIG. 4A is a cross-sectional view of a septa showing a structural example of a dividing line.
  • FIG. 4B is a cross-sectional view of a septa showing a structural example of a dividing line. 4A and 4B correspond to the cross-sectional view taken along line II of FIG. As shown in FIG. 3, the dividing lines 100 that facilitate separation of the dividing units can be provided as intermittent linear perforations 101 .
  • the perforations 101 can be formed in the vertical or horizontal direction so as to pass between the holes 20 on the main body 10 in a plan view of the main body 10 .
  • the perforations 101 can be formed as an array of perforations 101a penetrating vertically through the main body 10, as shown in FIG. 4A.
  • FIG. 4B it is possible to form an array of recessed recesses 101b that do not penetrate vertically through the main body 10 .
  • FIG. 4A shows a nozzle 400 as an example of a thin tube with a dashed line.
  • recesses 101b are provided on both the upper surface side and the lower surface side of main body 10.
  • the concave portion 101b on the upper surface side and the concave portion 101b on the lower surface side are provided at the same position so as to overlap each other when the main body portion 10 is viewed from above.
  • the recessed portion 101b may be provided only on the upper surface side of the main body portion 10 or may be provided only on the lower surface side of the main body portion 10 .
  • the perforations 101 can be formed by an appropriate method such as punching or resin molding using a molding die.
  • the perforations 101 are formed in a rectangular shape in plan view of the main body 10, but may be provided in an appropriate shape such as a circular shape, an elliptical shape, an oval shape, a diamond shape, a polygonal shape, or the like.
  • the length, width, depth, and pitch of the perforations 101a and the recesses 101b can be appropriately set as long as the division units are properly connected and easily separated when necessary.
  • the parting line 100 is provided as the perforation 101, even if the intermediate part of the parting line 100 is used as the starting point, it is possible to break the space between the perforations 101a or between the recesses 101b with a small force. Units can be easily separated from each other. Moreover, even if there is a place where the dividing lines 100 intersect each other, it is possible to accurately separate the desired dividing unit up to the place where it is separated.
  • FIG. 5 is a top view of a septa showing a structural example of a dividing line.
  • FIG. 6A is a cross-sectional view of a septa showing a structural example of a parting line.
  • FIG. 6B is a cross-sectional view of a septa showing a structural example of a dividing line. 6A and 6B correspond to cross-sectional views taken along line II-II in FIG. As shown in FIG. 5, the dividing line 100 that facilitates separation of the divisional units can be provided as a continuous linear groove 102 .
  • the grooves 102 can be formed vertically or horizontally so as to pass between the holes 20 on the main body 10 in a plan view of the main body 10 .
  • the groove 102 can be formed as a V-shaped triangular groove 102a in a cross-sectional view of the main body 10.
  • the body portion 10 can be formed as a thin portion 102b that is thinned.
  • the triangular groove 102a and the thin portion 102b are provided on both the upper surface side and the lower surface side of the body portion 10.
  • the triangular groove 102a and the thin portion 102b on the upper surface side and the triangular groove 102a and the thin portion 102b on the lower surface side are provided at the same position so as to overlap each other when the main body portion 10 is viewed from above.
  • the triangular groove 102a and the thin portion 102b may be provided only on the upper surface side of the main body portion 10, or may be provided only on the lower surface side of the main body portion 10.
  • the grooves 102 can be formed by an appropriate method such as cutting or resin molding using a molding die.
  • the thin portion 102b is thinned into a rectangular shape in a cross-sectional view of the main body portion 10, but may be formed in an appropriate shape such as a trapezoidal shape, a semicircular shape, a semielliptical shape, a semielliptical shape, a semipolygonal shape, or the like. can be reduced to The width and depth of the triangular groove 102a and the thin portion 102b can be appropriately set as long as the division units are appropriately combined and easily separated when necessary.
  • the main body part 10 formed in a sheet shape can be continuously torn along the parting line 100 with a small force, so that the divided units can be easily separated. . Also, even when cutting along the dividing line 100 with scissors, a cutter, or the like, the cutting edge is guided linearly, so that the desired dividing unit can be cut off accurately.
  • FIG. 7 is a top view of a septa showing a structural example of a dividing line.
  • FIG. 8 is a cross-sectional view of a septa showing a structural example of a dividing line.
  • FIG. 8 corresponds to a cross-sectional view taken along line III-III of FIG.
  • the dividing line 100 that facilitates the separation of the divisional units can be provided as a continuous linear cut 103 .
  • the cuts 103 can be formed in the vertical or horizontal direction so as to pass between the holes 20 on the main body 10 in a plan view of the main body 10 . As shown in FIG. 7, the cut 103 can be formed in a linear shape in which the inner walls are substantially in contact with each other. Moreover, as shown in FIG. 8, it is provided at a depth that does not penetrate the main body portion 10 .
  • the notch 103 is provided only on the upper surface side of the main body 10, but may be provided only on the lower surface side of the main body portion 10, or may be provided on both the upper surface side and the lower surface side of the main body portion 10. may be The notch 103 on the upper surface side and the notch 103 on the lower surface side are provided at the same position so as to overlap when the main body 10 is viewed from above.
  • the cut 103 can be formed by an appropriate method such as cutting or grooving.
  • the depth of the incision 103 can be any appropriate condition as long as the division units are appropriately combined and easily separated when necessary.
  • the cuts 103 are provided in a continuous line, but they may be provided in an intermittent line like perforations 101 .
  • the sheet-shaped body portion 10 can be continuously torn along the dividing line 100 with a small force, so that the divided units can be easily separated. Also, even with the method of pulling the divisional units to both outer sides with respect to the division line 100, the divisional units can be easily separated from each other because the ends of the cuts 103 are easily torn.
  • FIG. 9A is a top view of the septa showing a structural example of the end of the parting line.
  • FIG. 9B is a top view of the septa showing a structural example of the end of the dividing line.
  • FIG. 9C is a top view of a septa showing an example of the structure of the ends of the parting line.
  • a sheet-like main body in which a hole 20 and a tubular portion 30 are formed. 10 may be provided with a notch 105 at the end of the main body 10 that intersects the parting line 100 .
  • the notch 105 can be formed at the end of the main body 10 that intersects the perforation 101 so as to cut inward along the perforation 101 of the main body 10 .
  • the end of the main body 10 that intersects the groove 102 may be cut out along the groove 102 toward the inner side of the main body 10 .
  • the end portion of the main body 10 that intersects the cut 103 may be cut out along the cut 103 toward the inner side of the main body 10 .
  • the notch 105 can be formed by an appropriate method such as punching, cutting, or resin molding using a mold.
  • the notch 105 is cut in a V-shape in plan view of the main body 10, but has a rectangular shape, a trapezoidal shape, a semicircular shape, a semi-elliptical shape, and a semi-oval shape. It can be cut out in an appropriate shape such as a shape, a semi-polygonal shape, a cut shape, or the like.
  • the main body part 10 formed in a sheet shape can be easily torn along the parting line 100 with the notch 105 as a starting point. Also, even when cutting along the dividing line 100 with scissors, a cutter, or the like, the cutting edge is guided along the dividing line 100, so that the desired division unit can be cut off accurately.
  • FIG. 10 is a top view of the septa showing an example of the structure of the main body and the parting line.
  • the main body 10 of the septa 1 can also be provided in the form of a connecting sheet in which island-shaped portions 10a each having a hole 20 and a cylindrical portion 30 are connected to each other via a connecting portion 10b.
  • the divided unit constituted by the hole portion 20 and the cylindrical portion 30 constitutes a part of the single sheet-like main body portion 10, but a connecting sheet-like unit as shown in FIG. It may form part of the main body.
  • a structural unit that seals a container such as a well 230 provided on a microplate 200 in such a manner that a narrow tube such as a nozzle 400 of an automatic analyzer can be inserted and removed is separated by island-shaped portions 10a constituting a connecting sheet. can also
  • the body portion 10 of the septa 1 is formed by connecting island-shaped portions 10a each having a hole portion 20 and a cylindrical portion 30 formed therein.
  • the island-shaped portion 10 a is provided in a rectangular shape having a length and a width larger than the outer diameters of the hole portion 20 and the cylindrical portion 30 when the main body portion 10 is viewed from above.
  • the island-shaped portions 10a are connected to each other in a matrix through strip-shaped connecting portions 10b.
  • the connecting portion 10b is provided in a rectangular shape with a width smaller than that of the island-shaped portion 10a in plan view of the main body portion 10 .
  • Rectangular gaps larger than the perforations 101 shown in FIG. 3 are formed between the island-shaped parts 10a so as to be arranged in the vertical and horizontal directions.
  • a connecting portion 10b spans the space between the island-shaped portions 10a. If the connecting portion 10b is provided with a small tension as the maximum allowable load, it can be easily broken by manual pulling or the like. Therefore, such a gap functions as a dividing line 100 that facilitates separation between division units.
  • a connection sheet in which the island-shaped portions 10a are connected to each other can be formed by punching or resin molding using a mold.
  • the island-shaped portion 10a is provided in a rectangular shape in plan view of the main body portion 10 in FIG. 10, it may be provided in an appropriate shape such as a circular shape, a diamond shape, a polygonal shape, or the like.
  • the island-shaped portion 10a has one structural unit for sealing the wells 230 provided on the microplate 200 in such a manner that the fine tubes of the automatic analyzer can be inserted and removed. may
  • the length, width, and thickness of the island-shaped portion 10a can be set appropriately as long as the strength and flexibility of the body portion 10 are ensured.
  • connecting portion 10b is provided in a rectangular shape in plan view of the main body portion 10 in FIG. 10, it can be provided in an appropriate shape. Although one connecting portion 10b connects the island-shaped portions 10a in FIG. 10, a plurality of connecting portions 10b may be used.
  • the thickness of the connecting portion 10b can be equal to or smaller than the thickness of the island-shaped portion 10a.
  • the length, width, and pitch of the connecting portion 10b can be appropriately set as long as the division units are properly connected to each other and can be easily separated when necessary.
  • a perforation 101 is provided as a parting line 100 when the division unit constituted by the hole portion 20 and the cylindrical portion 30 forms a part of the connecting sheet-like body portion in which the island-shaped portions 10a are connected to each other.
  • the division units can be easily separated from each other as compared with the case. Even if the starting point is the intermediate portion of the gap between the island-shaped portions 10a, the space between the island-shaped portions 10a can be broken with a small force. Moreover, division into the smallest division units becomes easy.
  • the microplate 200 is a 96-well microplate
  • the septa 1 is for a 96-well microplate
  • 230 may be provided for microplates formed thereon. For example, it may be provided for a 24-well microplate, a 48-well microplate, a 384-well microplate, or the like.
  • the septa 1 is for microplates, but the septa 1 with a split structure is for a multiple container having a plurality of arranged container parts, for example, for multiple microtubes. Alternatively, it may be provided for multiple microvials, multiple electrophoresis medium containers of a capillary electrophoresis apparatus, or the like.
  • FIG. 11 is a perspective view showing a septa and multiple microtubes according to an embodiment of the present invention.
  • FIG. 11 shows a septa 2 for multiple microtube and a multiple microtube 300 to which the septa 2 is attached as an example of the septa according to this embodiment.
  • the multiple microtube 300 is used as a container for analyses, inspections, experiments, and the like.
  • the multiple microtube 300 is provided in a structure in which a plurality of microtubes 310 are connected in parallel.
  • Polyolefin such as polypropylene, polyethylene, and polystyrene is used as the material of the multiple microtube 300 .
  • the microtube 310 has a circular shape in a plan view, and is provided as a tapered, substantially cylindrical container. The upper portion of the microtube 310 is circularly opened upward. Inside the microtube 310, a cylindrical space is formed that tapers downward in diameter. The space inside the microtube 310 functions as a container, similar to the wells 230 of the microplate 200, and is filled with a desired liquid or the like.
  • the multiple microtube 300 is an octet microtube in which a total of eight microtubes 310 are connected.
  • the microtubes 310 are connected to each other via belt-shaped portions that are connected to the upper portions.
  • the number of connections, capacity, inner diameter, outer diameter, connection structure, etc. of the multiple microtube 300 may be any appropriate conditions.
  • the microtube 310 is provided as a tapered, substantially cylindrical container, but the shape of the microtube 310 is not particularly limited.
  • the microtube 310 may be provided as a substantially cylindrical microvial type or the like.
  • the multiple microtube 300 is used as a container for setting a sample in an automatic analyzer equipped with an autosampler.
  • the multiple microtube 300 can be supported by a rack or the like provided with a support hole for inserting the microtube 310 and set in an automatic analyzer.
  • the septa 2 As shown in FIG. 11, the septa 2 according to the present embodiment, like the septa 1 for microplates, has a sheet-like main body 10 and a plurality of holes penetrating vertically through the main body 10 . 20, bottomed tubular portion 30 formed so as to protrude downward from the periphery of each of hole portion 20 on the lower surface side of main body portion 10, and slits formed in the respective bottom portions of tubular portion 30 40 and .
  • the microtubes 310 of the multiple microtube 300 can be inserted and removed from the microtubes such as the capillaries, needles, and nozzles 400 of the automatic analyzer. It has the function of sealing in a state where it can be released.
  • the septa 2 is attached to the upper side of the multiple microtube 300 after a sample is placed in the microtube 310 of the multiple microtube 300 and before the multiple microtube 300 is set in an automatic analyzer.
  • the body portion 10, the hole portion 20, and the cylindrical portion 30 of the septa 2 can be integrally resin-molded with an elastic elastomer in the same manner as the microplate septa 1 described above.
  • Materials for the septa 2 include silicone rubber, fluororubber, ethylene-propylene-diene rubber (EPDM), and the like.
  • the main body 10 can have a width approximately equal to the outer diameter of the microtube 310 and a length approximately equal to the outer dimension of the microtube 310 in the connection direction.
  • the structural unit that seals the microtube 310 of the multiple microtube 300 in such a manner that the capillaries, needles, nozzles 400, etc. of the automatic analyzer can be inserted and extracted is the microplate unit.
  • the microplate unit can be provided in the same manner as the septa 1 of .
  • the septa 1 according to the present embodiment is provided with a slit 40 in the bottom of the tubular portion 30 that is opened in advance, similarly to the microplate septa 1 described above.
  • a projection 35 is provided on the side surface of the tubular portion 30 .
  • Other configurations can be provided as structures similar to the septa 1 for microplates described above.
  • septa 2 As in the septa 1 for the microplate described above, thin tubes such as the capillary, needle, and nozzle 400 of the automatic analyzer pass through the tubular portion 30 to the microtubes 310 of the multiple microtube 300. It is configured to adopt an insertion state in which the tubule is inserted and an extraction state in which the tubule is pulled out from the microtube 310 of the multiple microtube 300 to the outside of the cylindrical portion 30 .
  • a slit formed in the bottom of the cylindrical portion 30 is configured to open and close by elastic deformation.
  • the slits are opened by elastic deformation of the cylindrical portion 30 due to pressure from the narrow tube when the septa 2 shifts from the extracted state to the inserted state. Provided to allow insertion of tubules.
  • the elastic force of the cylindrical portion 30 closes the slit to seal the container such as the well 230 . .
  • the microtube 310 containing the sample can be sealed in a state in which the thin tube can be inserted and removed by the slit that opens and closes elastically. Therefore, while permitting the insertion of the capillary into the microtube 310 and the withdrawal of the capillary from the microtube 310, the evaporation of the components from the microtube 310 and the entry of contaminants into the microtube 310 are prevented. can be suppressed. Sealing the microtube 310 with the septa 2 enables accurate and stable analysis.
  • the hole portion 20 and the cylindrical portion 30 are in a state in which the microtube 310 of the multiple microtube 300 can be inserted and removed, and the capillary, needle, nozzle 400, etc. of the automatic analyzer can be inserted and removed. to form a structural unit to be sealed.
  • the septa 2 has a plurality of such structural units corresponding to the plurality of microtubes 310 connected to each other.
  • a plurality of hole portions 20 and cylindrical portions 30 formed on the main body portion 10 are provided so as to be divisible from each other in division units constituted by one or more hole portions 20 and cylindrical portions 30 .
  • the septa 2 is provided so that a plurality of structural units formed by the hole portion 20 and the cylindrical portion 30 can be divided and used separately.
  • a dividing line 100 is formed on the main body portion 10 of the septa 2 in the same manner as the septa 1 for microplates.
  • the division unit constituted by the hole portion 20 and the tubular portion 30 constitutes a part of the single sheet-like body portion 10 in which the hole portion 20 and the tubular portion 30 are formed.
  • the septa 2 shown in FIG. 11 is provided with a total of eight hole portions 20 and cylindrical portions 30 that can be divided one by one.
  • the septa 2 is provided with the hole portion 20 and the cylindrical portion 30 that can be divided into pieces. Also, the pattern of the division lines 100 that divide the division units can be appropriately provided according to the number of connections of the multiple microtubes 300 and the like.
  • the divided unit constituted by the hole portion 20 and the cylindrical portion 30 constitutes a part of the single sheet-like main body portion 10 .
  • dividing lines 100 for facilitating the separation of the dividing units are formed by perforations 101, grooves 102, notches 103, etc. , can be provided as an appropriate structure.
  • a notch 105 may be provided at the end of the main body 10 that intersects the parting line 100, as in the septa 1 for the microplate described above.
  • the divided unit constituted by the hole portion 20 and the cylindrical portion 30 constitutes a part of the single sheet-like main body portion 10, but the connecting sheet-like main body portion as shown in FIG. may be part of it.
  • the structural unit that seals the microtube 310 of the multiple microtube 300 in such a manner that the narrow tube of the automatic analyzer can be inserted and removed can also be separated by the island-shaped portion 10a that constitutes the connection sheet.
  • a structural unit is formed that seals containers such as wells and microtubes provided on a microplate in a state in which thin tubes such as capillaries, needles, and nozzles of automatic analyzers can be inserted and removed. Since the hole portion and the cylindrical portion are provided so as to be divisible from each other in divisional units composed of one or more hole portions and cylindrical portions, it is possible to divide these plural structural units and use them individually. can be done. Separable septa have a mechanically fragile structure that allows the main body to be easily broken. can be separated.
  • the division unit constituted by the hole portion and the cylindrical portion may be a form that constitutes a part of the single sheet-like main body or a form that constitutes a part of the connected sheet-like main body. Separability to the extent that units can be easily separated by manual pulling, etc., and connectivity to the extent that mutually connected divided units can be easily attached to a microplate can be achieved without combining different materials or members. , can be easily secured.
  • the present invention is not limited to the above-described embodiments, and various modifications are possible without departing from the scope of the present invention.
  • the present invention is not necessarily limited to having all the configurations included in the above embodiments. Replacing part of the configuration of one embodiment with another configuration, adding part of the configuration of one embodiment to another form, or omitting part of the configuration of one embodiment can be done.
  • the septa according to the above embodiments are for microplates and multiple microtubes.
  • the present invention can be applied to a container or a multiple container integrally provided with a plurality of arranged container parts.
  • the capacity of the container is not limited to the micro order, and can be applied to a container or container portion having an appropriate capacity.
  • septa 1 septa 2 septa 10 main body 10a island-shaped portion 10b connecting portion 20 hole 30 cylindrical portion 40 slit 100 parting line 101 perforation 102 groove 103 notch 105 notch 200 microplate 210 concave portion 220 outer frame 230 well (container) 231 upper part 232 lower part 300 multiple microtube 310 microtube (container) 400 Nozzle (Tube)

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne des cloisons (1) comprenant : plusieurs parties cylindriques (30) qui peuvent être ajustées dans les ouvertures d'une pluralité de récipients (230) qui ont été disposés en rangs ; et une fente (40) formée dans une partie inférieure de chaque partie cylindrique (30). Dans un état dans lequel les parties cylindriques (30) sont ajustées dans les ouvertures des récipients (230), les cloisons sont configurées pour se trouver dans un état d'insertion dans lequel un tube mince pour aspirer un liquide dans le récipient (230) ou évacuer le liquide de celui-ci est inséré dans le récipient (230) à travers la partie cylindrique (30), ou un état de retrait dans lequel le tube mince est retiré de l'intérieur du récipient (230) vers l'extérieur au-delà de la partie cylindrique (30). Les cloisons (1) sont telles que lors de la transition de l'état de retrait à l'état d'insertion, la fente (40) est amenée à s'ouvrir par une déformation élastique de la partie cylindrique (30) provoquée par la pression du tube mince, et ainsi l'insertion du tube mince est autorisée ; et lors de la transition de l'état d'insertion à l'état de retrait, la fente (40) est amenée à se fermer par une force élastique de la partie cylindrique (30), et ainsi le récipient (230) est scellé. Les parties cylindriques (30) sont disposées de telle sorte qu'il est possible de les séparer les unes des autres dans une unité de séparation configurée par une ou plusieurs parties cylindriques (30) au moyen d'une ligne de séparation (100).
PCT/JP2021/030988 2021-08-24 2021-08-24 Cloisons WO2023026361A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2021/030988 WO2023026361A1 (fr) 2021-08-24 2021-08-24 Cloisons

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2021/030988 WO2023026361A1 (fr) 2021-08-24 2021-08-24 Cloisons

Publications (1)

Publication Number Publication Date
WO2023026361A1 true WO2023026361A1 (fr) 2023-03-02

Family

ID=85321830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2021/030988 WO2023026361A1 (fr) 2021-08-24 2021-08-24 Cloisons

Country Status (1)

Country Link
WO (1) WO2023026361A1 (fr)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5910349A (ja) * 1982-06-18 1984-01-19 フエルナンド・サラバデ−ル・ミラマンダ 分析用容器の密栓
JPH07500048A (ja) * 1991-05-20 1995-01-05 クロシュアー・テクノロジーズ・インコーポレーテッド 一方向バルブを持つ封止キャップ
JPH10132713A (ja) * 1996-10-21 1998-05-22 Boehringer Mannheim Gmbh 汚染を伴わない熱化学反応工程の操作システム
JP2001097345A (ja) * 1999-09-27 2001-04-10 Sumitomo Pharmaceut Co Ltd 易分離性容器およびその製造方法
JP2001324474A (ja) * 2000-05-15 2001-11-22 Hitachi Ltd キャピラリアレイを用いた電気泳動装置及びそれに用いられるサンプルプレートアセンブリ
US6500390B1 (en) * 1996-10-17 2002-12-31 David A. Boulton Method for sealing and venting a microplate assembly
JP2012030863A (ja) * 2010-07-30 2012-02-16 Yoshino Kogyosho Co Ltd 連結容器
JP2017526362A (ja) * 2014-08-25 2017-09-14 アイデックス ラボラトリーズ インコーポレイテッドＩＤＥＸＸ Ｌａｂｏｒａｔｏｒｉｅｓ， Ｉｎｃ． マルチウェル試料試験装置およびそれを用いた試料試験方法
JP2020519440A (ja) * 2017-05-10 2020-07-02 イー・エム・デイー・ミリポア・コーポレイシヨン 可変圧縮封止部を備えるマルチウェル板

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5910349A (ja) * 1982-06-18 1984-01-19 フエルナンド・サラバデ−ル・ミラマンダ 分析用容器の密栓
JPH07500048A (ja) * 1991-05-20 1995-01-05 クロシュアー・テクノロジーズ・インコーポレーテッド 一方向バルブを持つ封止キャップ
US6500390B1 (en) * 1996-10-17 2002-12-31 David A. Boulton Method for sealing and venting a microplate assembly
JPH10132713A (ja) * 1996-10-21 1998-05-22 Boehringer Mannheim Gmbh 汚染を伴わない熱化学反応工程の操作システム
JP2001097345A (ja) * 1999-09-27 2001-04-10 Sumitomo Pharmaceut Co Ltd 易分離性容器およびその製造方法
JP2001324474A (ja) * 2000-05-15 2001-11-22 Hitachi Ltd キャピラリアレイを用いた電気泳動装置及びそれに用いられるサンプルプレートアセンブリ
JP2012030863A (ja) * 2010-07-30 2012-02-16 Yoshino Kogyosho Co Ltd 連結容器
JP2017526362A (ja) * 2014-08-25 2017-09-14 アイデックス ラボラトリーズ インコーポレイテッドＩＤＥＸＸ Ｌａｂｏｒａｔｏｒｉｅｓ， Ｉｎｃ． マルチウェル試料試験装置およびそれを用いた試料試験方法
JP2020519440A (ja) * 2017-05-10 2020-07-02 イー・エム・デイー・ミリポア・コーポレイシヨン 可変圧縮封止部を備えるマルチウェル板

Similar Documents

Publication Publication Date Title
EP1972945B1 (fr) Appareil de distribution de type soufflet et procédé
EP3011302B1 (fr) Système microfluidique ayant des organes de collecte de fluide
US9435765B2 (en) Cartridge and system for manipulating samples in liquid droplets
WO2016078340A1 (fr) Appareil, système, et procédé de distribution/mélange d'une petite quantité de liquide
US20060032746A1 (en) Method and device for contacting a microfluidic structure
WO2016011134A1 (fr) Cartouche microfluidique avec guide de pipetage
JP2004532099A (ja) 多ウェル装置
US11779925B2 (en) System, method, and device for forming an array of emulsions
WO2009024560A1 (fr) Appareil pour distribuer des substances pipetables
CN109746059B (zh) 微液滴生成系统
WO2006138743A2 (fr) Dispositif de transfert de fluide
KR20090007692A (ko) 필터 부착 마이크로 플레이트
CN111957361A (zh) 微滴制备系统、微流控芯片及其设计方法
EP2999778B1 (fr) Article, système et procédé de génération d'émulsion d'orifice commun
EP1103304A2 (fr) Récipient pour réactifs autoventilé et méthode pour introduire un réactif dans un analyseur ou un autre appareil
CN113649098B (zh) 微流控检测组件、样品混合装置及其应用
WO2023026361A1 (fr) Cloisons
WO2009129397A2 (fr) Distributeur à haut rendement
CA2130129A1 (fr) Fermeture a zone de perforation
WO2014108184A1 (fr) Cartouche et système de manipulation d'échantillons dans des gouttelettes liquides
JP2006208373A (ja) バリヤー貫通のためのリブを有するピペットチップを使用した液体採取
WO2023026359A1 (fr) Septum
US9829416B2 (en) Closure with septum strip
US20190255524A1 (en) Transfer arrays for simultaneously transferring multiple aliquots of fluid
US20220055028A1 (en) Fluid handling system

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21954421

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE