WO2022226746A1 - Poct血细胞分析仪及其使用方法 - Google Patents

Poct血细胞分析仪及其使用方法 Download PDF

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Publication number
WO2022226746A1
WO2022226746A1 PCT/CN2021/090055 CN2021090055W WO2022226746A1 WO 2022226746 A1 WO2022226746 A1 WO 2022226746A1 CN 2021090055 W CN2021090055 W CN 2021090055W WO 2022226746 A1 WO2022226746 A1 WO 2022226746A1
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WO
WIPO (PCT)
Prior art keywords
detection
pipette
blood cell
kit
pressure
Prior art date
Application number
PCT/CN2021/090055
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English (en)
French (fr)
Inventor
谭玉华
秦军芳
梁铁柱
Original Assignee
深圳市帝迈生物技术有限公司
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Application filed by 深圳市帝迈生物技术有限公司 filed Critical 深圳市帝迈生物技术有限公司
Priority to PCT/CN2021/090055 priority Critical patent/WO2022226746A1/zh
Publication of WO2022226746A1 publication Critical patent/WO2022226746A1/zh

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance

Definitions

  • the present application relates to the technical field of medical devices, in particular to a POCT blood cell analyzer, a pipette, a detection seat, a method for using the POCT blood cell analyzer, a kit, an assembly seat, a sample detection device, and a microporous sheet.
  • Blood cell analyzer also known as blood cell analyzer, blood cell analyzer, blood cell counter, etc.
  • Most of the components in the traditional blood cell analyzer belong to the cleaning system, because before the next sample tube test, the traces of the previous sample tube must be cleaned.
  • the entire cleaning system not only has a complex structure and many components, but also requires a large amount of reagents in the cleaning process, which takes a long time.
  • the POCT blood cell analyzer greatly simplifies the instrument components.
  • the POCT blood cell analyzer can completely remove the related components of the cleaning liquid path in the traditional blood analysis, which greatly reduces the complexity and production of the instrument. cost.
  • the present application provides a POCT blood cell analyzer, a pipette, a detection seat, a method for using the POCT blood cell analyzer, a kit, an assembly seat, a sample detection device, and a microwell sheet to at least partially solve the above technical problems.
  • a technical solution adopted in the present application is to provide a method for using a POCT blood cell analyzer, including: receiving a power-on trigger signal; performing hardware self-checking; The samples on the kit are subjected to automated sample pre-processing and sample testing is performed after the automated sample pre-processing is completed.
  • the beneficial effects of the present application are: different from the situation in the prior art, the present application provides a POCT blood cell analyzer, a pipette, a detection seat, a method for using the POCT blood cell analyzer, a kit, an assembly seat, and a sample detection device ,
  • the microporous sheet has a novel structure, is practical and reliable, has low cost, and can efficiently complete the fully automated detection.
  • FIG. 1 is a schematic three-dimensional structure diagram of a POCT blood cell analyzer provided in an embodiment of the present application
  • FIG. 2 is a schematic structural diagram of a module of a POCT blood cell analyzer provided in an embodiment of the present application
  • FIG. 3 is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in an embodiment of the present application.
  • FIG. 4 is a schematic diagram of the internal structure of a POCT blood cell analyzer provided in an embodiment of the present application, in which the pipette is cancelled;
  • FIG. 5 is a schematic diagram of a gas path structure of a pressure building system provided by an embodiment of the present application.
  • FIG. 6 is a schematic three-dimensional structure diagram of a pipette provided by an embodiment of the present application.
  • FIG. 7 is a schematic three-dimensional structural diagram of a puncture head provided by an embodiment of the present application.
  • FIG. 8 is a schematic bottom view of the metal shielding cover of the POCT blood cell analyzer and its transmission mechanism provided by an embodiment of the present application;
  • FIG. 9 is a schematic three-dimensional structural diagram of a detection seat of a POCT blood cell analyzer provided in an embodiment of the present application.
  • FIG. 10 is a schematic diagram of an exploded structure of a detection seat of a POCT blood cell analyzer provided in an embodiment of the present application;
  • FIG. 11 is a schematic side view of the structure of the conductive support and the conductive column of the POCT blood cell analyzer provided by another embodiment of the present application;
  • FIG. 12 is a schematic top-view structural diagram of a kit provided by an embodiment of the present application.
  • FIG. 13 is a simplified perspective perspective structural diagram of a POCT blood cell analyzer provided by another embodiment of the present application.
  • FIG. 14 is a flow chart of the use of the POCT blood cell analyzer provided in an embodiment of the present application.
  • the present application provides a POCT blood cell analyzer and a method of using the same.
  • the POCT blood cell analyzer includes a housing 10, a display screen 11, an outlet 12, a syringe 220, a rack 301, a pressure building system, a detection base 300 and its transmission mechanism, a pipette 400 and its transmission mechanism, metal shielding cover 500 and its transmission mechanism, etc.
  • the test seat 300 can reciprocate in and out of the bin 12 to facilitate loading or unloading of the kit 20.
  • the pipette 400 and its transmission mechanism are used to realize fully automated pre-processing of the sample to be tested (which can be pre-installed on the kit 20).
  • Fully automatic pre-processing includes automatic sample addition, automatic reagent addition (diluent, hemolytic reagent, dye lysis reagent, etc. are pre-installed on the kit 20), automatic mixing (bubbling or stirring), etc.
  • the pressure building system is used to provide The positive pressure and negative pressure required for the fully automated pretreatment of the pipette 400.
  • the metal shielding cover 500 and its transmission mechanism are used to cooperate with the pressure building system for automatic detection.
  • the air pressure connection device 510 of the pressure building system communicates with the pressure acting chamber 140 on the reagent kit 20 (the pressure acting chamber 140 is communicated with the rear pool of the impedance detection cell), and the pressure chamber 210 of the pressure building system starts Negative or positive pressure is provided so that the liquid in the front cell 120 of the impedance detection cell of the kit 20 flows through the micropores to the rear cell of the impedance detection cell of the kit 20 and the relevant parameters are recorded in the process.
  • the pneumatic connecting device 510 of the pressure building system is connected with the metal shielding cover 500 and moves synchronously.
  • the pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 arranged in linkage, a driver 224, an air filter 225, a first solenoid valve SV1, a second solenoid valve SV2, a third solenoid valve SV3, and a pneumatic connection. device 510, etc.
  • the pipette 400 includes an airway 401, an outer sleeve 402 sleeved on the outer periphery of the airway 401, a synchronization block 403, a light shield 404, an optical coupler 405, a fixing plate 406, a through slot 407, and is used for unloading the installation head (201 or 204) of the blocking member 4031 and so on.
  • the metal shielding cover 500 is installed on the rack 301 through the mounting frame 520, and is moved vertically by the motor 530 and the guide column 540.
  • the metal shielding cover 500 is in the shape of a cover with an opening at the bottom.
  • the air pressure connected to the pressure building system The connection device 510 extends into the metal shielding cover 500 .
  • the detection seat 300 includes a main body 310 , a conductive bracket 303 connected to the main body 310 , a metal shielding seat ( 320 , 321 , 322 ) covering the main body 310 , an optical detection component 330 embedded in the main body 310 , and the optical detection components are sequentially approached to the optical detection.
  • a Peltier 340 , a heat sink 350 , a fan 360 and the like are provided in the detection cavity of the component 330 .
  • the first embodiment as shown in FIG. 1 to FIG. 13 , this embodiment provides a POCT blood cell analyzer.
  • the POCT blood cell analyzer includes a housing 10 , a detection seat 300 , and a pipette 400 .
  • the detection base 300 can extend into or out of the housing 10 for receiving the reagent kit 20 provided with the impedance detection cell.
  • the kit 20 has a mounting head accommodating pool for mounting a mounting head (tip head 201 or piercing head 204).
  • the pipette 400 is arranged in the housing 10 and located above the detection seat 300, and is used to perform corresponding operations on the reagent kit 20, and the corresponding operations include pipetting operations, blowing bubbles and mixing operations, stirring and mixing operations, and piercing the sealing film. operate.
  • the pipette 400 moves to a preset position other than the mounting head accommodating pool (for example, the front pool 120 in FIG.
  • This embodiment also provides a POCT blood cell analyzer, which includes a housing 10 , a detection seat 300 , a pipette 400 and a detector (which may be a detection element such as an optocoupler 405 ).
  • the detection base 300 can extend into or out of the housing 10 for receiving the reagent kit 20 provided with the impedance detection cell and to be loaded, and the detection base 300 is provided with a power supply member for impedance detection.
  • the kit 20 has a mounting head accommodating pool for mounting the mounting head.
  • the pipette 400 is arranged in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent cartridge 20 .
  • the detector is used to detect whether the installation head is loaded on the pipette 400 when the POCT blood cell analyzer needs to unload the installation head. If it is detected that the installation head is loaded on the pipette 400, the pipette 400 moves to the installation head.
  • the unloading operation of the mounting head is performed at a preset position other than the setting pool. By setting the detector, it is possible to know whether there is a mounting head loaded on the pipette 400 and then selectively perform the unloading operation, which is relatively more intelligent.
  • This embodiment also provides a POCT blood cell analyzer, the POCT blood cell analyzer is used for receiving the kit 20 to be loaded, and the POCT blood cell analyzer includes a detection seat 300 and a pipette 400 .
  • the detection seat 300 is used for receiving the reagent box 20 provided with the impedance detection cell, and the detection seat 300 is provided with a power supply member for impedance detection.
  • the kit 20 has a mounting head accommodating pool for mounting the mounting head.
  • the pipette 400 is arranged above the detection base 300 and is used to load the mounting head to perform corresponding operations on the reagent cartridge 20.
  • the pipette 400 moves to the mounting head accommodating pool or to a location other than the mounting head accommodating pool.
  • the preset position performs the mount header unloading operation.
  • the POCT blood cell analyzer further includes a detector (which may be a detection element such as an optocoupler 405), and the detector is used to detect whether a mounting head is loaded on the pipette 400 when the POCT blood cell analyzer needs to unload the mounting head , if it is detected that the pipette 400 is loaded with a mounting head, the pipette 400 unloads the mounting head to the mounting head accommodating pool or a preset position other than the mounting head accommodating pool.
  • the pipette 400 is easier to unload the mounting head to a preset position other than the mounting head accommodating pool, that is, directly find a large-diameter pool to unload it, which can avoid the mounting head already installed in the mounting head accommodating pool. It can also shorten the running path when unloading the mounting head and reduce the alignment requirements when unloading the mounting head at the same time.
  • the reagent kit 20 has a plurality of pool bodies, and the preset position is one of the pool bodies.
  • the plurality of pool bodies include a mounting head accommodating pool matched with the mounting head and several functional pools, and the pipette 400 unloads the mounting head into any functional pool.
  • the functional pool may include the diluent pool 111, the front pool 120 (WBC detection pool or RBC detection pool), etc.
  • WBC detection pool or RBC detection pool diluent pool
  • RBC detection pool RBC detection pool
  • the pipette 400 includes an air tube 401 and an outer sleeve 402 .
  • the outer sleeve 402 moves axially relative to the air tube 401 , the mounting head can be unloaded.
  • This embodiment also provides a method for using the POCT blood cell analyzer, which includes:
  • the pipette 400 of the POCT blood cell analyzer moves to the mounting head accommodating pool to load the mounting head for the first detection related operations
  • the mounting head used in the first test needs to be unloaded, and the pipette 400 of the POCT blood cell analyzer moves to the installation A header unload operation is performed at a preset location other than the header holding pool.
  • the using method can ensure that the pipette 400 will not repeatedly place the mounting head when the mounting head is already installed in the mounting head accommodating pool, and can also shorten the running path when the mounting head is unloaded, and at the same time reduce the alignment when unloading the mounting head Require.
  • This embodiment also provides a method for using the POCT blood cell analyzer, which includes:
  • the pipette 400 of the POCT blood cell analyzer moves to the mounting head accommodating pool to load the mounting head for the first detection related operations
  • the mounting head used in the first test needs to be unloaded, and the pipetting of the POCT blood cell analyzer can be detected by the detector. Whether the installation header is loaded on the device 400;
  • the pipette 400 moves to a preset position outside the mounting head accommodating pool to perform the unloading operation of the mounting head.
  • This method of use can know whether a mounting head is loaded on the pipette 400 and then selectively perform an unloading operation, which is relatively more intelligent.
  • This embodiment also provides a method for using the POCT blood cell analyzer, including:
  • the pipette of the POCT blood cell analyzer moves to the mounting head accommodating pool to load the mounting head for the first detection
  • the kit 20 and the mounting head used in the first test need to be taken out. Therefore, it is necessary to test whether the POCT blood cell analyzer is A kit 20 is installed;
  • the detector Before the second detection, the detector can detect whether the pipette 400 of the POCT blood cell analyzer is loaded with a mounting head;
  • the pipette 400 is controlled to move to a preset position other than the mounting head accommodating pool or the mounting head accommodating pool.
  • Mount header uninstall operation When you select a preset location other than the mounting head storage pool to perform the mounting head uninstallation operation, the mounting head will not be placed repeatedly when the mounting head storage pool is already installed with the mounting head, and the running path when uninstalling the mounting head can also be shortened. Also reduces alignment requirements when unloading the mounting head.
  • the preset position may correspond to the inside of the detection base 300 or to the outside of the detection base 300 . That is to say, the unloading operation is not limited to unloading the mounting head to the test seat 300 or to the reagent box 20, but also to the area inside the instrument other than the test seat 300, for example, there is a mounting head inside the instrument to recover Maintenance personnel regularly empty the mounting head recovery box.
  • the mounting head can be unloaded by axial movement of the outer sleeve 402 of the pipette 400 relative to the air tube 401 of the pipette 400 .
  • the pipette 400 unloads the mounting head through a fixed blocking member or a follow-up telescopic blocking member 4031, and the blocking member 4031 is a telescopic motor, a vertical or horizontal electromagnet or a U-shaped mouth
  • the baffle plate wherein the outer sleeve 402 can be connected with a synchronization block 403 , the blocking member 4031 blocks the synchronization block 403 and when the pipette 400 rises, the mounting head is unloaded by the lower end of the outer sleeve 402 .
  • the pipette 400 includes an air tube 401 and a detection element.
  • the air tube 401 is used for loading the installation head (tip head 201 or the puncture head 204 ), and the detection piece is provided on the side of the air tube 401 for detecting whether the air tube 401 is loaded with the installation head.
  • the pipette 400 further includes an outer sleeve 402, the outer sleeve 402 is sleeved on the outer circumference of the airway 401, when the airway 401 is loaded with the installation head, the airway 401 is inserted into the installation head, so that the installation head pushes the outer sleeve 402 along the airway 401.
  • a positional change occurs in the axial direction of the trachea, and the detection piece is used to detect the position of the outer sleeve 402 to determine whether a mounting head is sleeved on the airway 401 .
  • the outer sleeve 402 When the installation head is sleeved on the airway tube 401, the outer sleeve 402 is located at the first position of the airway tube 401 (the state position shown in FIG. 6 ). The second position, the first position is higher than the second position.
  • a light blocking sheet 404 is connected to the outer sleeve 402, and the detection element is an optical coupler 405 matched with the light blocking sheet 404.
  • the optical channel of the optical coupler 405 is blocked by the light blocking sheet 404, it means that a mounting head is sleeved on the air duct 401
  • the optical channel of the optical coupler 405 is not blocked by the light blocking plate 404
  • the pipette 400 includes a fixing plate 406 arranged on the side of the air tube 401 , and the optical coupler is arranged on the fixing plate 406 .
  • the air conduit 401 of the pipette 400 can move up and down independently, and the pipette 400 as a whole can move in two or three dimensions.
  • the fixing plate 406 is provided with a through slot 407, a pair of optical transceivers of the optocoupler 405 protrude into the through hole and are located on the side of the air guide tube 401, the outer sleeve 402 is also connected with a synchronization block 403, and the synchronization block 403 extends into the through slot 407, When the air guide tube 401 rises and the synchronizing block 403 is blocked, the installation head is unloaded.
  • the outer sleeve 402 is still sleeved on the outer circumference of the air guide pipe 401, and since the synchronization block 403 extends into the through groove 407, the outer sleeve 402 is opposite to the air guide pipe 401. It does not fall off, but is naturally supported at the bottom of the through groove 407 (ie, the second position).
  • This embodiment also provides a method for using the POCT blood cell analyzer, comprising the following steps:
  • the outer circumference of the air guide 401 is sleeved with an outer sleeve 402, and the position of the outer sleeve 402 is detected by the detection piece to determine whether the air guide 401 is sleeved. Install the header.
  • the outer sleeve 402 When the installation head is sleeved on the airway tube 401, the outer sleeve 402 is located at the first position of the airway tube 401. When the installation head is not sleeved on the airway tube 401, the outer sleeve 402 is located at the second position of the airway tube 401. The first position is higher than the second position of the airway tube 401. Second position.
  • the detection piece is an optocoupler 405
  • the outer sleeve 402 is correspondingly provided with a light blocking plate 404 matched with the optocoupler 405 .
  • the light blocking plate 404 blocks the optical channel of the optical coupler 405 , it is determined that the air guide tube 401 is sleeved with the installation head outer sleeve 402 .
  • This embodiment provides a POCT blood cell analyzer, which includes a housing 10 , a detection seat 300 , a pipette 400 , and a detection piece.
  • the detection seat 300 can extend into or out of the housing 10, and is used for receiving the reagent kit 20 provided with the impedance detection cell; the pipette 400 is arranged in the housing 10 and located above the detection seat 300, and the pipette 400 includes Airway tube 401, airway tube 401 is used to load the installation head (tip head 201 or puncture head 204); the detection piece is arranged on the side of airway tube 401 to detect whether the installation head is loaded on the airway tube 401, and the detection piece can be The photocoupler 405 or the like can detect the presence or absence of an object.
  • the setting of the detector can know the loading state of the mounting head, so as to prevent the pipette from contaminating the internal air circuit of the POCT blood cell analyzer by pipetting in the state where the mounting head is not loaded.
  • this embodiment provides a POCT blood cell analyzer
  • the POCT blood cell analyzer includes a detection seat 300, a pipette 400 disposed above the detection seat 300, and a pressure builder System
  • the detection base 300 is used for receiving the reagent kit 20 to be loaded
  • the reagent box 20 is provided with a front pool 120 and a rear pool that are communicated through micropores
  • the pressure building system includes a pressure chamber 210, a pneumatic connection device 510, a first syringe 222, a second Two syringes 223 , and a driving member 224 .
  • the first syringe 222 communicates with the pressure chamber 210 or the pipette 400 , and is used for establishing positive pressure or negative pressure on the pressure chamber 210 , and is also used for assisting the pipette 400 to perform pipetting, or for the liquid in the front pool 120 .
  • Bubble mixing is performed, the second syringe 223 is communicated with the pipette 400, and is used for aspiration and discharge of samples and reagents, and the driving member 224 is used for simultaneously driving the first syringe 222 and the second syringe 223, the first syringe 222 and the second syringe 222.
  • the injector 223 can be a linkage type injector, which is convenient to share the same driving member 224.
  • the driving member 224 can be a motor.
  • the first injector 222 and the second injector 223 can also be separate injectors that are independently controlled by different motors.
  • the POCT blood cell analyzer further includes a solenoid valve, and the solenoid valve includes a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.
  • the first solenoid valve SV1 selectively communicates the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively communicates the first solenoid valve SV1 with the pressure chamber 210 and the outside atmosphere, and the outside atmosphere is filtered through the air.
  • the device 225 and the pipeline T10 are connected to the second solenoid valve SV2; the third solenoid valve SV3 selectively communicates the air pressure connection device 510 with the pressure chamber 210.
  • the third solenoid valve SV3 When the third solenoid valve SV3 is energized, the pipelines T8 and T7 pass through the third solenoid valve SV3 Connected.
  • the first syringe 222 can inhale through the air filter 225, the T10 pipeline, the second solenoid valve SV2, the T5 pipeline, the first solenoid valve SV1, and the T1 pipeline, and then pass through the T1 pipeline, the first solenoid valve SV1, and the T5 pipeline.
  • the pipeline, the pipeline of the second solenoid valve SV2 and the pipeline of T6 establish a positive pressure to the pressure chamber 210 .
  • the first syringe 222 can suck the gas in the pressure chamber 210 through the T1 pipeline, the first solenoid valve SV1, the T5 pipeline, the second solenoid valve SV2, and the T6 pipeline to establish a negative pressure, and then pass through the T1 pipeline, the first Solenoid valve SV1, T5 pipeline, second solenoid valve SV2, T10 pipeline, air filter 225 exhaust.
  • the positive pressure or negative pressure can be output through the T7 pipeline, the third solenoid valve SV3 , the T8 pipeline, and the pneumatic connection device 510 .
  • the T8 pipeline can use a relatively hard pipeline, that is, the hardness of the T8 pipeline is greater than that of the second catheter 221, and the hardness of the T8 pipeline is larger to prevent the tube walls of the T8 pipeline from sticking together when the negative pressure is output. Block the internal airflow passage of the T8 line.
  • the first solenoid valve SV1 connects the T1 pipeline with the T2 pipeline
  • the positive or negative pressure generated by the first syringe 222 and the second syringe 223 acts on the pipette 400 at the same time, that is, when a large amount of sample aspiration or push is required
  • the first syringe 222 can play a role of assisting pipetting.
  • the volume of the first syringe 222 is larger than that of the second syringe 223.
  • the first syringe 222 is mainly used for pressure building, and the second syringe 223 is mainly used for sample pushing/suction. Of course, the two can also be used simultaneously for pressure building as needed. Or push/aspirate.
  • the ratio of the volume of the first syringe 222 to the volume of the second syringe 223 may be 80-120:1, for example, the volume of the first syringe 222 is 10 ml, and the volume of the second syringe 223 is 100 microliters.
  • the driving member 224 drives the piston rods of the first syringe 222 and the second syringe 223 through the linkage plate 231 so that the first syringe 222 and the second syringe 223 move synchronously.
  • the piston rod of the second syringe 223 is in sealing fit with the rear end of the outer cylinder of the second syringe 223 .
  • the POCT blood cell analyzer also includes a housing 10 and a frame 301.
  • the pressure chamber 210 is arranged on the housing 10 or on the frame 301.
  • the pipette 400, the first syringe 222, the second syringe 223, and the driving member are arranged on the on the rack 301.
  • the detection base 300 is slidably or rotated relative to the rack 301 and is positioned below the pipette 400 .
  • the POCT blood cell analyzer also includes a metal shielding cover 500.
  • the detection seat 300 can move to the liquid dispensing station relative to the frame 301 (ie, the position shown in FIG. 3) so as to facilitate the corresponding operation of the pipette 400.
  • the detection seat 300 is opposed to the machine
  • the rack 301 can move to the sample detection station (not shown in the figure, corresponding to the directly below the metal shielding cover 500), and the metal shielding cover 500 is used to descend at the sample detection station to cooperate with the detection seat 300 to form a relative
  • the closed metal cavity further shields the external electromagnetic interference.
  • the rack 301 can form a double-layer structure, the lower layer is provided with the detection seat 300, and the upper layer is provided with the pipette 400, the metal shielding cover 500 and the syringe 220.
  • the housing 10 is provided with a display screen 11 and a warehouse outlet 12.
  • the detection seat 300 is used to move to the warehouse outlet 12 to receive the reagent cartridge 20 into the detection seat 300.
  • the liquid dispensing station and the warehouse outlet The distance of 12 is smaller than the distance between the sample detection station and the warehouse outlet 12, that is to say, the warehouse outlet 12 faces the inside of the instrument as a liquid dispensing station and a sample detection station in sequence.
  • the detection seat 300 reciprocates between the loading station, the liquid dispensing station and the sample detection station, and the liquid dispensing station is located between the loading station and the sample detection station. In other implementations, the liquid dispensing station and the sample detection station may also be the same station.
  • This embodiment also provides a method for using the aforementioned POCT blood cell analyzer, including the following steps:
  • the pressure release of the pressure chamber 210 is controlled to apply positive pressure to the front pool 120 or negative pressure to the back pool through the pneumatic connection device 510 to realize that the liquid in the front pool 120 flows to the back pool through the micropores.
  • the POCT blood cell analyzer provided in this embodiment is reasonable in layout, compact in structure, and convenient to use.
  • the kit 20 includes a box body 100 and a microfluidic detection sheet 600 .
  • the box body 100 includes an impedance detection cell (including a front cell 120, a back cell, and a pressure action chamber 140 that are connected in communication) for performing impedance detection.
  • an impedance detection cell including a front cell 120, a back cell, and a pressure action chamber 140 that are connected in communication
  • the microfluidic detection sheet 600 is integrally connected with the box body 100, assembled or not connected, and is located at the side of the impedance detection cell.
  • the microfluidic detection chip 600 is provided with a sample injection hole 601 to receive the sample and then lay it flat in the microfluidic detection chip 600 .
  • the box body 100 further includes a diluent pool 111, a hemolyzing agent pool 106, an optical detection pool, and a dyeing and lysing pool 602.
  • the diluting solution pool 111 is used to encapsulate the diluent
  • the hemolyzing agent pool 106 is used to encapsulate the lysing agent
  • the dyeing and lysing pool 602 is used to encapsulate the hemolytic agent.
  • the dyeing and lysis reagent is packaged, and the optical detection cell is integrally connected or assembled to the box body 100 .
  • the box body 100 further includes a mounting head accommodating pool and a sample accommodating pool, the mounting head accommodating pool is used for placing the mounting head (tip head 201 or) the puncturing head 204, and the sample accommodating pool is used for placing the sample tube.
  • this embodiment also provides a POCT blood cell analyzer, the POCT blood cell analyzer includes a detection base 300 , an image recognition base 610 , and an image recognition device 620 .
  • the detection base 300 is provided with a power supply member (such as the conductive holder 303 shown in FIG. 9 ) for impedance detection, the detection base 300 is used for receiving the aforementioned reagent box 20 and the image recognition base 610 is arranged on the side of the detection base 300
  • the image recognition device 620 is disposed above the image recognition base 610 , and is used for cooperating with the image recognition base 610 to perform image detection on the microfluidic detection sheet 600 .
  • the POCT blood cell analyzer further includes a pipette 400, and the pipette 400 is arranged above the detection seat 300.
  • the POCT blood cell analyzer includes a metal shielding cover 500 matched with the detection seat 300, and the metal shielding cover 500 can perform a lifting motion or a rotating motion , the image recognition device 620 is set independently or connected with the metal shielding cover 500 .
  • the POCT blood cell analyzer and the kit 20 provided in this embodiment can support impedance detection and microfluidic image detection at the same time, and the joint detection efficiency is optimized and the effect is good.
  • the fifth embodiment please refer to FIG. 1 to FIG. 13 together.
  • This embodiment provides a POCT blood cell analyzer.
  • the POCT blood cell analyzer includes a rack 301 , a detection base 300 , and a pipette 400 .
  • the detection seat 300 is slidably arranged or rotated relative to the rack 301 , the detection seat 300 reciprocates in the loading station and the sample detection station, and the detection seat 300 slides out or rotates out of the rack 301 and is located at the loading station to receive the kits 20 loaded into or allow the cartridge 20 to be removed.
  • the pipette 400 is arranged in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent cartridge 20 .
  • the POCT blood cell analyzer also includes a liquid dispensing station; the liquid dispensing station is located between the loading station and the sample detection station; or the liquid dispensing station and the sample detection station are the same station.
  • the detection seat 300 is in a semi-enclosed shape with an upper opening, and the detection seat 300 is snap-fitted with the reagent box 20 .
  • the POCT blood cell analyzer also includes a metal shield cover 500 matched with the metal shield seats (320, 321, 322).
  • the metal shielding cover 500 is disposed above the detection base 300 and is configured to move in a vertical direction at the sample detection station, and the vertical edge of the metal shielding cover 500 abuts the upper surface of the metal shielding base (320).
  • the POCT blood cell analyzer also includes a pressure building system.
  • One end of the pressure building system is provided with a pneumatic connection device 510 extending into the metal shielding cover 500 and moving synchronously with the metal shielding cover 500 .
  • Action chamber 140 is provided.
  • This embodiment provides a method for using a POCT blood cell analyzer, and the method includes the following steps:
  • the detection base 300 slides out or rotates out of the rack 301 to receive the reagent cartridge 20 into the loading station;
  • the detection seat 300 is slid or screwed into the frame 301 and moved to the dispensing station;
  • the pipette 400 moves to load the pre-installed mounting head (tip head 201 or puncture head 204) on the reagent cartridge 20 to perform corresponding operations to complete the dispensing;
  • the detection seat 300 is moved from the liquid dispensing station to the sample detection station under the metal shielding cover 500;
  • the metal shielding cover 500 moves downward to cooperate with the detection seat 300;
  • the pneumatic connection device 510 connected to the metal shielding cover 500 starts to provide pressure to drain the liquid in the impedance detection cell on the reagent kit 20 to perform impedance detection.
  • the POCT blood cell analyzer and its using method provided in this embodiment have a high degree of automation, and the pretreatment and liquid dispensing operations of the liquid to be tested are automatically completed inside the instrument, avoiding randomness and errors of manual operations.
  • the POCT blood cell analyzer includes a detection seat 300 and a metal shielding cover 500 .
  • the detection seat 300 reciprocates at the loading station and the sample detection station, and is used for receiving the reagent box 20 with the impedance detection cell into the loading station. When the seat 300 moves to the sample detection station, it is covered with the detection seat 300 for electromagnetic signal shielding.
  • the POCT blood cell analyzer also includes a liquid dispensing station; the liquid dispensing station is located between the loading station and the sample detection station; or the liquid dispensing station and the sample detection station are the same station.
  • the detection seat 300 is in a semi-enclosed shape with an upper opening, and the metal shielding cover 500 is in a semi-enclosed shape with a lower opening.
  • the detection seat 300 is snap-fitted with the reagent box 20 .
  • Metal shielding bases (320, 321, 322), the detection base 300 is arranged to be able to translate or rotate under the metal shielding cover 500, and the metal shielding cover 500 is arranged to be able to move up and down in the vertical direction.
  • the POCT blood cell analyzer also includes a pressure building system.
  • One end of the pressure building system is provided with a pneumatic connection device 510 extending into the metal shielding cover 500 and moving synchronously with the metal shielding cover 500 .
  • Action chamber 140 is provided.
  • This embodiment also provides a method for using a POCT blood cell analyzer, the method comprising the following steps:
  • the detection base 300 slides out or rotates out of the rack 301 to receive the reagent cartridge 20 into the loading station;
  • the detection seat 300 is slid or screwed into the frame 301 and moved to the dispensing station;
  • the pipette 400 performs corresponding operations on the reagent cartridge 20 at the dispensing station;
  • the metal shielding cover 500 and the detection seat 300 are covered and combined to perform impedance detection. Specifically, the detection seat 300 first moves under the metal shielding cover 500 , and then the metal shielding cover 500 moves downward to cover the detection seat 300 .
  • the pipette 400 dispensing liquid to the kit 20 includes: the pipette 400 absorbs the pre-installed blood samples and reagents in the kit 20 and transfers them into the detection pool of the kit 20, wherein the reagents include hemolytic agents and diluents.
  • the POCT blood cell analyzer and its using method provided in this embodiment have a high degree of automation, and the pretreatment and liquid dispensing operations of the liquid to be tested are automatically completed inside the instrument, avoiding randomness and errors of manual operations.
  • the detection seat 300 includes a detection cavity 302 and a conductive support 303 .
  • the detection chamber 302 is used for receiving the reagent box 20 provided with the impedance detection cell, and the reagent box 20 is provided with electrodes, and the conductive support 303 is used for electrically connecting the electrodes.
  • the electrodes are conductive posts 122 disposed on opposite sides of the reagent cartridge 20 .
  • the conductive support 303 includes a downwardly inclined elastic piece 304 , and the elastic piece 304 is used to support and electrically connect the conductive post 122 .
  • the conductive bracket 303 further includes a fixed connection part 305 integrally connected with the elastic piece part 304 .
  • the shape of the fixed connection part 305 is not limited, and a fixing hole can be provided to facilitate the fastening and assembly by screws.
  • the conductive support 303 includes a fixed connection part 305 that is integrally connected and arranged vertically and a bending part 306 that is bent and extended (which can be a V-shaped bending or an arc-shaped bending part). Bending), when the conductive post 122 is assembled from top to bottom, the bent part 306 is squeezed out, and the bent part 306 is reset and abutted with the conductive post 122 when the conductive post 122 is assembled in place.
  • the conductive support 303 of this form has better elastic deformation ability , durable and able to maintain reliable electrical connection for a long time.
  • the conductive support 303 can be one and disposed on one side of the conductive column 122 , or two can be disposed on both sides of the conductive column 122 to further increase the reliability of the electrical connection.
  • the bent portion 306 may include a first bent portion 3061 and a second bent portion 3062 that are connected to the fixed connection portion 305 in sequence. The bent portion 3062 abuts against the conductive post 122 when the conductive post 122 is assembled in place.
  • the detection seat 300 further includes a main body 310 and a metal shielding seat (320, 321, 322).
  • the main body 310 is provided with a detection cavity 302, and the side wall of the main body 310 is provided with an assembly groove 307 that communicates with the detection cavity 302, and a conductive bracket 303
  • the metal shielding seat (320, 321, 322) extends into the detection cavity 302 through the assembly groove 307, and the metal shielding seat (320, 321, 322) is covered on the outer periphery of the main body portion 310, and is insulated from the conductive bracket 303.
  • the metal shielding seat (320, 321, 322) includes a Side plates ( 320 , 321 ) around the body portion 310 and a bottom plate ( 322 ) attached to the bottom of the body portion 310 .
  • the kit 20 also includes an integrally connected or detachable optical detection cell, the detachable optical detection cell can be inserted through the rectangular insertion hole 107, and the detection base 300 further includes an optical detection assembly 330 corresponding to the optical detection cell.
  • 330 includes a light-emitting assembly, a light-receiving assembly, and a detection cavity located between the light-emitting assembly and the light-receiving assembly, and the included angle between the axis of the light-emitting assembly and the axis of the light-receiving assembly is 0 to 60 degrees, specifically 0 degrees, 20 degrees, 30 degrees, 45 degrees, 60 degrees, etc.
  • the optical detection components 330 are in multiple groups, and the light source cavities of the multiple optical detection components 330 are separated from each other by the light blocking surrounding walls 333 to avoid mutual interference of detection light.
  • the multiple sets of optical detection components 330 may include two sets of laser detection components 331 and a set of LED light source detection components 332.
  • a light blocking surrounding wall 333 is provided between the detection cavities of the laser detection components 331, and the light blocking surrounding wall 333 preferably acts as a Black treatment, so that the light absorption effect is better and the reflection effect is poor, so as to avoid the reflected light from affecting the normal optical detection.
  • the detection base 300 further includes a Peltier 340, a heat sink 350 and a fan 360 arranged in sequence near the detection cavity of the laser detection component 331.
  • the heat sink 350 includes a heat absorbing substrate 351 attached to the Peltier 340 and a heat absorbing substrate 351.
  • the vertically connected heat dissipation fins 352 and the fan 360 can be arranged at one end of the heat dissipation fin 352 away from the heat absorbing substrate 351 , and the detection temperature can be controlled by setting the Peltier 340 , the heat sink 350 and the fan 360 , under the preset detection temperature
  • the test results obtained by the test are relatively more accurate.
  • the bottom of the detection chamber 302 is provided with a tact switch 334.
  • the tact switch 334 can know the loading status of the reagent cartridge 20. When the reagent cartridge 20 is installed, the tact switch 334 is pressed. The touch switch 334 is in a pop-up state.
  • This embodiment also provides a POCT blood cell analyzer, the POCT blood cell analyzer includes a pipette 400, the aforementioned detection seat 300, and a pipette 400 disposed above the detection seat 300, and the pipette 400 is used to align the kit 20 to perform the corresponding operations.
  • the conductive support 303 of the POCT blood cell analyzer and its detection base 300 provided in this embodiment includes a downwardly inclined elastic piece 304, which has good adaptability and stability when it is electrically connected with the electrodes of the kit 20. Greatly reduces the possibility of poor contact.
  • the eighth embodiment provides a method for using a POCT blood cell analyzer, including the following steps:
  • the pipette 400 is controlled to move above the first mounting head accommodating pool of the reagent cartridge 20 and descend to load the first mounting head, wherein the first mounting head may be the tip head 201;
  • the control metal shielding cover 500 is covered with the detection seat 300 to establish a communication relationship between the rear pool of the reagent kit 20 and the pressure building system;
  • the liquid to be tested is detected by impedance detection method.
  • controlling the pipette 400 to move above the first mounting head accommodating pool of the reagent cartridge 20 and descend to load the first mounting head includes: controlling the pipette 400 to move three-dimensionally relative to the first mounting head accommodating pool to make pipetting The device 400 and the first mounting head are pressed against each other to load the first mounting head.
  • Moving the sample into the forecourt 120 includes: three-dimensional movement of the pipette 400 relative to the fore-pool 120 and use of the inner cavity of the first mounting head as a translocation in the liquid to suck the diluent and/or the sample.
  • the volume of the inner cavity of the first mounting head is generally It is 100 microliters to 1000 microliters, and generally does not exceed 4/5 of the volume when used to avoid contamination of the pipeline of the liquid entering the pressure building system.
  • Mixing the liquid to be tested in the front pool 120 includes the pipette 400 sucking and spitting the liquid to be tested through the first mounting head; or the pipette 400 injects air bubbles into the liquid to be tested through the first mounting head; or pipetting The device 400 stirs the liquid to be tested through the first installation head.
  • the pipette 400 is further included to unload the first mounting head to the first mounting head accommodating tank.
  • the pipette 400 After the pipette 400 unloads the first mounting head to the first mounting head accommodating pool, it further includes that the pipette 400 moves above the second mounting head accommodating pool and descends to load the second mounting head.
  • the pipette 400 After the pipette 400 is moved to the second mounting head receptacle and lowered to load the second mounting head, it includes:
  • the pipette 400 moves to the sample tube/sample dilution pool/other sample placement place to move the sample into the light detection cell;
  • the liquid to be tested is detected by an optical detection method.
  • the pipette 400 After the pipette 400 is moved to the second mounting head receptacle and lowered to load the second mounting head, it includes:
  • the pipette 400 is moved to the sample tube, and the sample is moved into the staining and lysis tank 602 for incubation, and then moved into the microfluidic detection sheet 600;
  • the particles in the microfluidic detection sheet 600 are detected by an image detection method.
  • the POCT blood cell analyzer includes a detection base 300, a pipette 400, a pressure building system, a metal shielding cover 500, and a transmission assembly.
  • the detection seat 300 is used for receiving the kit 20 to be loaded, and the kit 20 is provided with a front pool 120 and a back pool connected through micropores, and is provided with a first mounting head, a diluent, and a sample;
  • the pipette 400 is used to load the first mounting head and move the diluent and the sample into the front pool 120;
  • the metal shielding cover 500 is used for covering with the detection seat 300, and the pressure building system is used for guiding the liquid to be tested from the front tank 120 into the rear tank.
  • the transmission assembly is used to drive the detection base 300, the pipette 400 and the metal shielding cover 500 to perform one-dimensional, two-dimensional or three-dimensional movement. , synchronous belt, etc.
  • the reagent kit 20 is also provided with a second installation head and an optical detection cell, and the pipette 400 is also used to load the second installation head and move the sample into the optical detection cell for optical detection.
  • the kit 20 is further provided with a second mounting head, a dyeing and lysing reagent, a microfluidic detection sheet 600
  • the POCT blood cell analyzer further includes an image detection base 610 and an image detection device 620 arranged at intervals
  • the pipette 400 also includes It is used to load the second mounting head and move the sample into the dye lysis reagent for incubation, and then move the incubated sample into the microfluidic detection sheet 600, and conduct the microfluidic detection sheet 600 through the image detection base 610 and the image detection device 620.
  • Image detection is used to load the second mounting head and move the sample into the dye lysis reagent for incubation, and then move the incubated sample into the microfluidic detection sheet 600, and conduct the microfluidic detection sheet 600 through the image detection base 610 and the image detection device 620.
  • the POCT blood cell analyzer and its using method provided in this embodiment have a high degree of automation, complete automatic liquid dispensing inside the instrument, have good process operation consistency, and can support impedance detection, optical detection, and image detection at the same time.
  • the ninth embodiment please refer to FIG. 1 to FIG. 13 together, this embodiment provides a POCT blood cell analyzer, the POCT blood cell analyzer includes a detection seat 300 , a puncture head loading mechanism, a puncture head 204 , and a pipette 400 .
  • the puncture head loading mechanism may be an independent mechanism, or may be the same mechanism as the pipette 400 .
  • the detection seat 300 is used for loading the reagent box 20, and the reagent box 20 is provided with a diluent pool and a hemolyzing agent pool, and the openings of the diluent pool and the hemolyzing agent pool are provided with a sealing film.
  • the puncture head 204 is used to puncture at least two sealing films on the reagent box 20 loaded into the POCT blood cell analyzer one by one, and the puncture head loading mechanism is used to load the puncture head 204 .
  • the puncture head 204 is arranged on the reagent box 20 and/or the detection seat 300 .
  • the reagent cartridge 20 and/or the detection base 300 is provided with a placement position matched with the puncture head 204 , and the puncture head 204 is loaded by the pipette 400 and then moves synchronously with the pipette 400 .
  • the puncture head 204 is further swung horizontally after passing through the sealing film, so that the diameter of the through hole formed in the sealing film can be enlarged.
  • At least two sealing films are located on the same straight line or the same arc, so that the trajectory of puncturing one by one can be relatively simple.
  • the puncture head 204 includes a main body part 2042 and a sharp part 2041 , and the sharp part 2041 is provided at one end of the main body part 2042 .
  • the cross-section of the sharp part 2041 can be in the shape of a straight line, a cross shape, a square shape, a Y shape, or the like.
  • One end of the main body portion 2042 away from the sharp portion 2041 is provided with a flange portion 2044.
  • the flange portion 2044 can facilitate the hanging of the puncture head 204 in the puncture head accommodating pool 104, and can also form a stable abutting fit with the outer sleeve 402.
  • the tenth embodiment please refer to FIG. 1 to FIG. 13 together.
  • This embodiment provides a POCT blood cell analyzer.
  • the POCT blood cell analyzer includes a detection base 300 and an installation head.
  • the detection seat 300 is used for loading the reagent box 20, and the reagent box 20 is provided with a diluent pool and a hemolyzing agent pool, and the openings of the dilution pool and the hemolyzing agent pool are provided with a sealing film, and the installation head is used for loading the POCT blood cell analyzer.
  • the sealing film on the kit 20 is punctured one by one.
  • the mounting head is a tip head 201 or a dedicated puncture head 204 , and the mounting head is provided on the reagent box 20 .
  • the reagent kit 20 is provided with a placement position matching the mounting head, the outer diameter of the puncture head 204 is larger than the outer diameter of the tip head 201, and the opening formed after the puncture head 204 passes through the sealing film is larger than the diameter of the tip head 201 extending into the opening, This can prevent the formation of a negative pressure cavity in the pool when the tip is sucking liquid, and ensure the accuracy of liquid suction.
  • the POCT blood cell analyzer further includes a pipette 400 , and the mounting head is loaded by the pipette 400 and then moves with the pipette 400 .
  • At least two sealing films are located on the same straight line or the same arc.
  • the trajectory of the puncturing one by one can be relatively simple.
  • the mounting head After passing through the sealing film, the mounting head further swings horizontally to enlarge the diameter of the through hole on the sealing film.
  • the tip 201 can first pierce the first through hole with the first stroke, and then pierce the second through hole with the second stroke to perform sample suction.
  • the first stroke is smaller than the second stroke, so that liquid suction can be avoided.
  • the formation of a negative pressure sealed cavity affects the accuracy of liquid suction.
  • the tip is lifted after piercing the through hole, and then the sample is aspirated, which can also avoid the formation of a negative pressure sealed cavity during liquid suction, which affects the accuracy of liquid suction.
  • the puncture head 204 includes a main body portion 2042 and a sharp portion 2041 provided at the end of the main body portion 2042 .
  • the cross section of the sharp part 2041 is in the shape of a straight line, a cross shape, a square shape, a Y shape, or the like.
  • a flange portion 2044 is provided at one end of the main body portion 2042 away from the sharp portion 2041 .
  • the tip head 201 may be directly used for puncturing, or a dedicated puncture head 204 may be used for puncturing.
  • the eleventh embodiment please refer to FIG. 1 to FIG. 13 together.
  • This embodiment provides a blood cell analyzer for POCT, which includes a housing 10 , a pipette 400 , and a puncture head 204 .
  • the pipette 400 is provided in the housing 10, the pipette 400 includes an airway 401, the puncture head 204 includes a main body portion 2042 and a sharp portion 2041, the sharp portion 2041 is arranged at one end of the main body portion 2042, and the other end of the main body portion 2042 is arranged There is a accommodating cavity 2045 into which the airway tube 401 is inserted.
  • the sharp part 2041 is used to pierce the sealing film on the kit 20 when the POCT blood cell analyzer performs automatic detection.
  • the cross section of the sharp part 2041 is in-line, cross, rice-shaped or Y-shaped.
  • a flange part 2044 is provided at the end of the main body part 2042 away from the sharp part 2041 to facilitate hanging on the box body 100 of the kit 20 .
  • the POCT blood cell analyzer further includes a detection seat 300, the detection seat 300 is used to install the reagent box 20, the reagent box 20 is provided with a puncture head placement pool, and the flange portion 2044 is supported on the outer periphery of the puncture head placement pool.
  • the pipette also includes an outer sleeve 402 sleeved on the outer periphery of the airway 401, the lower end of the outer sleeve 402 is in contact with the flange portion 2042, and one end of the main body portion 2042 close to the flange portion 2044 is provided with a plurality of extending toward the sharp portion 2041. Ribs 2043 to increase strength.
  • the puncture head 204 can be integrally formed with metal or plastic material.
  • the present embodiment provides a reagent kit 20 .
  • the reagent kit 20 includes a plurality of pool positions, one of which is used to place the aforementioned puncture head 204 .
  • the plurality of pool positions include the diluent pool 111 and/or the hemolyzing agent pool 106, and the diluent pool 111 and/or the hemolyzing agent pool 106 are provided with a sealing film (not shown).
  • the cell position also includes a WBC detection cell and/or an RBC detection cell for impedance detection.
  • the kit 20 further includes a microfluidic detection sheet 600 , and the microfluidic detection sheet 600 can perform image detection through the image detection base 610 and the image detection device 620 .
  • the puncturing operation of the sealing film can be performed without manual operation, and a dedicated puncturing head 204 or tip 201 can be used to achieve better consistent operation.
  • the twelfth embodiment please refer to FIG. 1 to FIG. 13 together.
  • This embodiment provides a POCT blood cell analyzer.
  • the POCT blood cell analyzer includes a detection seat 300 and a pressure building assembly.
  • the detection base 300 is used to install the reagent kit 20.
  • the reagent kit 20 includes an impedance detection cell for performing impedance detection.
  • the impedance detection cell includes a fore cell 120 and a rear cell that are communicated through micropores.
  • the pressure building assembly is connected to the fore cell 120 and the back cell. /or the air circuit of the back cell is used to provide pressure so that the liquid to be tested in the fore cell 120 flows to the back cell through the micropores, and is also used to provide pressure to the front cell 120 or the back cell before the liquid to be tested is injected into the fore cell 120 so that the The micropores are unblocked.
  • the pressure building component provides negative pressure
  • impedance method detection can be performed.
  • the micropores can be recoiled to ensure the smoothness of the micropores.
  • the pressure building assembly includes a syringe 220 and a pressure chamber 210 that are communicated with the air circuit.
  • the syringe 220 is used to establish positive or negative pressure for the pressure chamber 210 .
  • the pressure building component is used to provide air flow to realize bubble mixing of the liquid to be tested or to make the micropores unblocked, specifically, air blowing or suction through the front cell 120/back cell to make the micropores unblocked.
  • the POCT blood cell analyzer includes multiple sets of gate valves, and the gate valves are connected to the pressure chamber 210 and the syringe 220 .
  • the POCT blood cell analyzer includes a metal shielding cover 500 matched with the detection base 300 .
  • the metal shielding cover 500 is provided with an air pressure connection device 510 communicating with the pressure chamber 210 or the syringe 220 , and the air pressure connection device 510 is used for connecting with the back or front pool 120 . Connected.
  • the metal shielding cover 500 is arranged to move in the vertical direction.
  • the POCT blood cell analyzer further includes a pipette 400 , and the pipette 400 is disposed above the detection seat 300 and is used to perform corresponding operations on the reagent cartridge 20 .
  • the pipette 400 is in air communication with the pressure building assembly through a pipeline, and the pipette 400 is used to load the mounting head on the reagent cartridge 20 and perform corresponding operations through the mounting head.
  • This embodiment also provides a method for using the POCT blood cell analyzer, which includes the following steps:
  • the receiving kit is loaded into the detection base 300, the kit includes an impedance detection cell for performing impedance detection, and the impedance detection cell includes a front cell 120 and a rear cell that are communicated through micropores;
  • the pressure is provided to the fore cell 120 or the back cell to make the micropores unblocked, which may specifically include: providing pressure to the fore cell 120 or the back cell by the pressure in the pressure chamber 210 of the pressure building component to make the micropores unblocked.
  • the pores are unblocked; or the micropores are unblocked by applying pressure to the front pool 120 or the back pool through the syringe 220 of the pressure building assembly.
  • the thirteenth embodiment provides a POCT blood cell analyzer.
  • the POCT blood cell analyzer includes a detection seat 300, a human-computer interaction module and a processor.
  • the detection seat 300 is used for The receiving kit 20 is loaded, the kit 20 is provided with at least two item detection pools, the detection seat 300 is provided with an auxiliary detector for detecting the items corresponding to the item detection pools, and the human-computer interaction module communicates with the detection seat 300 Connection, used to select detection items, the human-computer interaction module is a combination of touch screen or display screen 11 and mechanical buttons, the processor is connected with the detection base 300 and the human-computer interaction module signal, and is used to receive detection items and auxiliary detectors.
  • the item detection pool is detachably mounted on the reagent box 20, and the auxiliary detector is used to detect whether there is a corresponding item detection pool.
  • the auxiliary detector includes an optical detection component 330.
  • the optical detection component 330 includes a light-emitting component and a light-receiving component.
  • the light signal obtained by the light-receiving component is used to assist in determining whether the item detection cell on the kit 20 corresponds to the selected detection item.
  • the auxiliary detector includes a tact switch 334, and the signal obtained by the tact switch 334 is used to assist in determining whether the item detection pool on the reagent cartridge 20 corresponds to the selected detection item.
  • the auxiliary detector includes an image recognition base 610 and an image recognition device 620 that are relatively spaced apart.
  • the signals obtained by the image recognition base 610 and the image recognition device 620 are used to assist in determining whether the item detection pool on the kit 20 corresponds to the selected detection item.
  • the POCT blood cell analyzer further includes a code scanner, and the code scanner is used to obtain label information corresponding to the reagent kit 20 , and the label information may include information such as name, type, production time, and the like.
  • This embodiment also provides a method for using the POCT blood cell analyzer, comprising the following steps:
  • the processor determines whether the detection bit information corresponds to the selected item to be detected. If not, a corresponding prompt is output. If it corresponds, the follow-up operation can be performed normally. Select the correct inspection item.
  • the corresponding detection position information of the reagent box 20 includes a scattered light signal, a transmitted light signal or an electrical signal triggered by a micro switch.
  • the items to be tested include at least one of blood routine testing, CRP testing, SAA testing, and blood cell classification testing.
  • This embodiment also provides a method for using the POCT blood cell analyzer, including:
  • the solution provided in this embodiment compares the items that the user wants to detect with the items that the kit can actually support before detection, which can better prevent fools.
  • the fourteenth embodiment please refer to FIG. 1 to FIG. 13 together.
  • This embodiment provides a POCT blood cell analyzer.
  • the POCT blood cell analyzer includes a housing 10, a detection seat 300, a pipette 400, and a first conduit. 211 and the anti-folding part 212 .
  • the detection base 300 can extend into or out of the housing 10, and is used for receiving the reagent kit 20 provided with the impedance detection cell to be loaded. ).
  • the pipette 400 is arranged in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent cartridge 20 .
  • the first conduit 211 is in pneumatic communication with the pipette 400 , and the other end of the first conduit 211 is in communication with a pressure building system (eg, the second syringe 223 in FIG. 5 ).
  • the pressure building system may include a syringe 220 and a pressure chamber 210 At least one of a solenoid valve (SV1, SV2, SV3), a multi-way joint, and a manifold, the anti-folding member 212 is combined with the first conduit 211 to prevent the first conduit 211 from being excessively bent to block the first conduit 211 the internal air channel.
  • the inner diameter of the first conduit 211 is relatively thin, generally ranging from 0.5 mm to 1.5 mm, which is prone to excessive bending and obstruction in actual use.
  • the anti-folding member 212 can be a sleeve sleeved on the outer periphery of the first catheter 211, and the hardness of the sleeve is less than or equal to the hardness of the first catheter 211.
  • the hardness of the sleeve can also be greater than the hardness of the first catheter 211.
  • the wall thickness of the sleeve can be greater than or equal to the wall thickness of the first catheter 211, and the bending resistance of the sleeve can be less than or equal to the bending resistance of the first catheter 211, so that the sleeve can be reduced.
  • the tube wears the first conduit 211 , and at the same time prevents the first conduit 211 from being excessively bent to block the inner air passage of the first conduit 211 .
  • the section of the sleeve is circular, or the section of the sleeve is C-shaped.
  • the anti-folding member 212 can also be a spring sleeved on the outer circumference of the first conduit 211 .
  • the anti-folding member 212 can also be a strap wrapped around the outer circumference of the first conduit 211 , and the strap can be a cloth strap, a plastic strap, or a metal strap.
  • the end of the first conduit 211 is provided with a joint part, and the joint part can be arranged at one or both ends of the first conduit 211.
  • the joint part can be a clamp, a clamp joint, a flanged joint, Straight fitting or threaded fitting.
  • the POCT blood cell analyzer provided in this embodiment is provided with an anti-folding member 212 on the outer periphery of the relatively thin first conduit 211 , which can better avoid the occurrence of a blocked air path and improve the stability of the instrument.
  • this embodiment provides a POCT blood cell analyzer
  • the POCT blood cell analyzer includes a housing 10, a detection base 300, a pipette 400, a pressure building system, The first conduit 211 and the second conduit 221.
  • the detection base 300 can extend into or out of the housing 10, and is used for receiving the reagent kit 20 provided with the impedance detection cell to be loaded. ).
  • the pipette 400 is arranged in the housing 10 and located above the detection seat 300 , and is used to perform corresponding operations on the reagent cartridge 20 .
  • Both ends of the first conduit 211 are respectively connected to the pipette 400 and the second syringe 223.
  • the second conduit 221 connects the syringe 220, the pressure chamber 210, and the solenoid valves (SV1, SV2, SV3), and the first conduit 211 (Fig.
  • the inner diameter of the T3 pipeline in Fig. 5 is smaller than or equal to the inner diameter of the second conduit 221 (ie, the pipelines except T3 and T4 in FIG. 5 ), and the hardness of the first conduit 211 is greater than that of the second conduit 221 .
  • the function of the first conduit 211 is mainly to control the accuracy of sample suction or push-out, and the error caused by gas compression is required to be small, so the hardness is large and the inner diameter is relatively small, while the function of the second conduit 221 is mainly to build pressure, It is required to be fast, and the inner diameter can be relatively large.
  • the ratio of the inner diameter of the first conduit 211 to the inner diameter of the second conduit 221 may be greater than 0.5 and less than or equal to 1.
  • the ratio of the length of the first conduit 211 to the length of the second conduit 221 is greater than 1 and less than or equal to 1.5.
  • the relatively long first conduit 211 can facilitate the installation of joints (eg, clamp joints). That is, the first conduit 211 is relatively thin and hard, and the second conduit 221 is relatively thick and soft.
  • the POCT blood cell analyzer further includes an anti-folding member 212 , which is combined with the first conduit 211 to prevent the first conduit 211 from being excessively bent to block the internal air passage of the first conduit 211 .
  • the anti-folding element 212 can be a sleeve or a spring sleeved on the outer periphery of the first conduit 211 .
  • the hardness of the sleeve is less than or equal to the hardness of the first catheter 211.
  • the hardness of the sleeve can also be greater than the hardness of the first catheter 211.
  • the hardness of the first conduit 211 is relatively large, and the deformation of the first conduit 211 itself is relatively small when the air pressure in the first conduit 211 changes, which can improve the accuracy of suction and discharge operations, and the wall thickness of the casing can be greater than or equal to
  • the wall thickness of the first conduit 211 and the bending resistance of the sleeve may be less than or equal to the bending resistance of the first conduit 211 .
  • the section of the sleeve is circular or C-shaped.
  • the anti-folding element 212 can also be a band wrapped around the outer circumference of the first conduit 211 .
  • the straps are cloth straps, plastic straps or metal straps.
  • the hardness of the first conduit 211 is greater than or equal to the hardness of the second conduit 221, the hardness of the first conduit 211 is relatively large, and the deformation of the first conduit 211 itself is relatively small when the air pressure in the first conduit 211 changes, thereby improving the suction.
  • the wall thickness of the first conduit 211 is less than or equal to the wall thickness of the second conduit 221 ; the bending resistance of the first conduit 211 is less than or equal to the bending resistance of the second conduit 221 .
  • the inner diameter and hardness are selected according to two different requirements, which can meet the requirements in a targeted manner, and realize the accuracy and efficiency of the instrument, and the anti-folding member 212 is designed for the thin first catheter 211, which can better It avoids the occurrence of air path blockage, and further improves the stability of the instrument.
  • the sixteenth embodiment please refer to FIG. 1 to FIG. 14.
  • This embodiment provides a method for using a POCT blood cell analyzer. The method may include the following steps:
  • S10 Receive a power-on trigger signal, specifically, the user presses a mechanical button to turn on the power;
  • S11 perform hardware self-check, that is, check and feedback each voltage and current output by each port on the circuit board;
  • S12 to determine whether the pipette is loaded with a mounting head, it can be judged by detecting elements such as an emission photocoupler or a reflective photocoupler, and the mounting head can be a tip head or a puncture head;
  • the pipette unloads the mounting head, and the unloading position can be a specific position inside the instrument, or it can be some pool positions on the reagent box 20 inside the instrument;
  • the initialization means that each moving part is reset to zero position or the zero position is confirmed and the pressure is cleared to zero.
  • the kit 20 can be received and loaded, and the whole process is carried out in the POCT blood cell analyzer. Automated sample preprocessing (automatic mixing of samples and corresponding reagents), and sample detection after the automated sample preprocessing is completed.
  • the pipette after the pipette unloads the mounting head, it includes:
  • the detection seat 300 is taken out of the warehouse for the reagent cartridge 20 to be taken out. In this case, it is usually an accidental power failure during the last use, and the mounting head and the reagent cartridge 20 are still left inside the instrument;
  • the detection seat 300 is returned to the warehouse and then the whole machine initialization step is performed. This step is to ensure that there are no external components (the mounting head and the reagent box 20) in the machine, and then it is convenient for the subsequent startup process actions to not be caused by the tip head or the reagent box 20. affected by the existence of .
  • the step of judging whether the pipette is loaded with a mounting head also includes: judging whether a reagent cartridge 20 is installed in the detection seat 300.
  • a reagent cartridge 20 is installed in the detection seat 300.
  • the reagent cartridge 20 and the mounting head will exist inside the instrument at the same time, so Whether there is a mounting head in the instrument can only be determined, or whether there is a mounting head and a reagent cartridge 20 in the instrument at the same time, wherein the determination of the reagent cartridge 20 can be determined by means of a tact switch in the detection seat 300 or the like.
  • the POCT blood cell analyzer provided in this application may be provided with one, two or more pressure chambers 210, and a positive pressure or negative pressure can be established through a syringe pressure and reach a certain range of pressure values.
  • the pressure self-test process is carried out after initialization.
  • the pressure self-test needs to build a negative pressure of a certain value.
  • the pressure can be built from -25 to 30kpa. It is composed of a pressure sensor, a pressure chamber 210 and a gas pipeline.
  • the pressure building process is as follows: During the pressure building process, the pressure sensor constantly detects the pressure of the pressure chamber 210.
  • the syringe motor drives the syringe to pull out at the maximum speed, and at the same time, the solenoid valve is opened to establish a negative pressure operation.
  • the syringe is directly connected to the pressure chamber. 210. No other ventilation ports are opened in the pressure chamber 210, and a negative pressure can be established when the syringe is pulled out. If the syringe motor has reached the maximum stroke, that is, after the syringe has been pulled to the maximum range, the target pressure has not yet been built, then close the solenoid valve. At this time, the syringe is initialized at the maximum speed with the maximum distance.
  • the solenoid valve After the initialization is completed, open the solenoid valve and simultaneously Continue to pull out the syringe to build pressure until the target pressure is reached. If the target pressure is not reached at the end of the pressure building period, a pressure build-up fault will be reported. At this point the pressure build is completed.
  • the pressure self-test continues, and when the target pressure is established and the pressure sensor detects normal, the solenoid valve is opened, the interior of the pressure chamber 210 is communicated with the air, and the negative pressure is released. After the pressure self-test, perform a simple blank test without placing the reagent cartridge 20, and directly measure the signal under no-load condition to ensure the normal test performance of the POCT blood cell analyzer. Next, the main measurement process is performed.
  • S15 perform signal detection, including judging the detection signal after the electromagnetic shielding component is matched to determine the electromagnetic shielding effect and judging whether the current and voltage signals are within the threshold range, wherein the signal detection may include various photoelectric signals, etc., and also includes metal shielding cover and After the metal shielding seat is combined and closed, the impedance method is simulated and tested, and the shielding effect of the metal shielding cover and the metal shielding seat or the signal quality of the circuit board itself can be judged according to the output result.
  • the step of entering the standby state includes:
  • S17 Receive a shutdown trigger signal, that is, the user presses the shutdown button. In order to prevent misoperation, it can be set that after the user presses the shutdown button, the screen displays whether it is determined to shutdown;
  • S31 receive sample ID setting and measurement mode setting.
  • options such as mode setting may be provided on the screen, and some optional items are provided for the user to select, such as CRP detection, SAA detection, blood detection, etc.;
  • receiving a trigger signal for starting the test that is, a touch button or a physical button may be provided on the screen or on the instrument to generate a trigger signal for starting the test;
  • step S33 check whether the information to be measured (such as reagent information, or scan code comparison/read IC card comparison) matches the measurement mode through a pop-up window, if not, click cancel (S46) and return to step S31 to receive the sample ID setting and measurement mode setting;
  • information to be measured such as reagent information, or scan code comparison/read IC card comparison
  • step S38 check the presence or absence of the reagent box 20, if not, go back to step S36, and prompt to put the reagent box 20;
  • test result specifically, the test result can be output through the display screen or the printing device;
  • the usage method of the POCT blood cell analyzer provided in this embodiment is reasonable and orderly, and can quickly perform detection of a single item or simultaneous detection of multiple items.
  • This embodiment also provides a POCT blood cell analyzer, which includes a housing 10 , a detection base 300 and a pipette 400 .
  • the detection seat 300 can extend into or out of the housing 10 for receiving the loading of the reagent cartridge 20 and reciprocates between the loading station of the reagent cartridge 20 and the sample detection station; the pipette 400 is arranged on the casing 10 Inside and above the detection seat 300 , it is used to perform automated sample preprocessing on the kit 20 .
  • the kit 20 includes a front pool 120 and a back pool that are communicated through micropores.
  • the POCT blood cell analyzer also includes a pressure building system.
  • the pressure building system is installed in the housing 10 and used to make the liquid to be tested flow from the front pool 120 to the back pool for impedance. detection method.
  • the POCT blood cell analyzer further includes metal shielding seats (320, 321, 322) for covering with the detection seat 300, the kit 20 includes a pressure action chamber 140 communicating with the back pool, and the metal shielding seats (320, 321, 322) ) is provided with an air pressure connection device 510 connected to the pressure building system.
  • the metal shielding seat (320, 321, 322) is covered with the detection seat 300, the air pressure connection device 510 is sealed with the pressure action chamber 140.
  • the pipette 400 is provided with a detection element (for example, the optocoupler 405 shown in FIG. 6 ).
  • the pipette 400 includes an airway 401 and an outer sleeve 402 sleeved on the outer periphery of the airway 401.
  • the airway 401 is used for loading the device.
  • the pipette 400 also includes a head retraction mechanism (refer to the aforementioned blocking member 4031 ), which is used to stop the outer sleeve 402 from rising when the airway 401 rises, so that the outer sleeve 402 unloads the mounting head.
  • a head retraction mechanism (refer to the aforementioned blocking member 4031 ), which is used to stop the outer sleeve 402 from rising when the airway 401 rises, so that the outer sleeve 402 unloads the mounting head.
  • the head-retracting mechanism is an electromagnet, a motor, or a U-shaped mouth baffle set relative to the axial or radial direction of the air duct 401 .
  • the pressure building system includes a pressure chamber 210, a first syringe 222 and a second syringe 223 arranged in linkage, the first syringe 222 is used to establish positive pressure or negative pressure for the pressure chamber 210, and the second syringe 223 is communicated with the pipette 400, using When the pipette 400 is sleeved with the mounting head, the inner cavity of the mounting head is used as a liquid transfer position to aspirate the reagent and/or the sample.
  • the POCT blood cell analyzer includes a first conduit 211 and a second conduit 221, the first conduit 211 is connected between the second syringe 223 and the pipette 400, the second conduit 221 is used for connecting the syringe and the pressure chamber 210, the first conduit 211
  • the inner diameter is smaller than the inner diameter of the second conduit 221 .
  • An anti-folding member 212 is provided on the outer periphery of the first conduit 211, and the anti-folding member 212 is a sleeve, a spring or a strap.
  • This embodiment also provides a POCT blood cell analyzer, which includes a test seat 300, a pipette 400 disposed above the test seat 300, and a pressure building system.
  • a POCT blood cell analyzer which includes a test seat 300, a pipette 400 disposed above the test seat 300, and a pressure building system.
  • the pressure building system includes a pressure chamber 210, a pneumatic connection device 510, a first syringe 222, a second syringe 223, and a driving member 224. , the first solenoid valve SV1, the second solenoid valve SV2, and the third solenoid valve SV3.
  • the pneumatic connection device 510 is connected with the pressure chamber 210, and is used for applying positive pressure or negative pressure to the front pool 120 or the back pool to realize the liquid in the front pool 120 flowing to the back pool;
  • the first syringe 222 is communicated with the pressure chamber 210/pipette 400 , used to establish positive or negative pressure on the pressure chamber 210 , and also used to assist the pipette 400 in pipetting, or to bubble and mix the liquid in the front pool 120 ;
  • the second syringe 223 is connected to the pipette 400 Commonly used for pipetting;
  • the driving part is used to drive the first syringe 222 and the second syringe 223 at the same time;
  • the first solenoid valve SV1 selectively communicates the first syringe 222 with the pressure chamber 210 and the second syringe 223;
  • the second solenoid valve SV2 The first solenoid valve is selectively communicated with the pressure chamber 210 and the external

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Abstract

一种POCT血细胞分析仪的使用方法，包括：接收开机触发信号；进行硬件自检；进行整机初始化；接收试剂盒装入；对试剂盒上的样本进行自动化样本前处理并在自动化样本前处理完成后进行样本检测。

Description

POCT血细胞分析仪及其使用方法 技术领域
本申请涉及医疗器械技术领域，特别涉及一种POCT血细胞分析仪、移液器、检测座、POCT血细胞分析仪的使用方法、试剂盒、装配座、样本检测装置、微孔片。
背景技术
血细胞分析仪又叫血液细胞分析仪、血球仪、血球计数仪等，是医院临床检验应用非常广泛的仪器之一。传统的血液细胞分析仪内很大部分的部件都属于清洗系统，因为在下一次样本管检测之前，必须保证将上一次样本管的使用痕迹清洗干净。整个清洗系统不仅结构复杂，部件多，而且清洗过程需要使用大量的试剂，占用较长的时间。
POCT血细胞分析仪相对传统血细胞分析仪在仪器组件上做了极大的简化，POCT血细胞分析仪可以将传统血液分析中的清洗液路相关组件完全去除，极大的降低了仪器的复杂度和生产成本。
然而，现有的POCT血细胞分析仪有的功能过于简单，有的结构较为复杂，有的自动化程度低，有的较为昂贵。
实用新型内容
本申请提供一种POCT血细胞分析仪、移液器、检测座、POCT血细胞分析仪的使用方法、试剂盒、装配座、样本检测装置、微孔片，以至少部分解决上述技术问题。
为解决上述技术问题，本申请采用的一个技术方案是：提供一种POCT血细胞分析仪的使用方法，包括：接收开机触发信号；进行硬件自检；进行整机初始化；接收试剂盒装入；对试剂盒上的样本进行自动化样本前处理并在自动化样本前处理完成后进行样本检测。
本申请的有益效果是：区别于现有技术的情况，本申请提供的一种POCT血细胞分析仪、移液器、检测座、POCT血细胞分析仪的使用方法、试剂盒、装配座、样本检测装置、微孔片结构新颖、实用可靠、成本低廉、能够高效的完成全自化检测。
附图说明
为了更清楚地说明本实施例中的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本申请的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图，其中：
图1是本申请一实施例提供的POCT血液细胞分析仪的立体结构示意图；
图2是本申请一实施例提供的POCT血液细胞分析仪的模块结构示意图；
图3是本申请一实施例提供的POCT血液细胞分析仪的内部结构示意图；
图4是本申请一实施例提供的POCT血液细胞分析仪的内部结构示意图，其中取消显示了移液器；
图5是本申请一实施例提供的建压系统的气路结构示意图；
图6是本申请一实施例提供的移液器的立体结构示意图；
图7是本申请一实施例提供的穿刺头的立体结构示意图；
图8是本申请一实施例提供的POCT血液细胞分析仪的金属屏蔽盖及其传动机构的仰视结构示意图；
图9是本申请一实施例提供的POCT血液细胞分析仪的检测座的立体结构示意图；
图10是本申请一实施例提供的POCT血液细胞分析仪的检测座的爆炸结构示意图；
图11是本申请另一实施例提供的POCT血液细胞分析仪的导电托与导电柱配合的侧面结构示意图；
图12是本申请一实施例提供的试剂盒的俯视结构示意图；
图13是本申请另一实施例提供的POCT血液细胞分析仪的简化立体透视结构示意图；
图14是本申请一实施例提供的POCT血液细胞分析仪的使用流程图。
具体实施方式
下面将结合本申请实施例中的附图，对本申请实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅是本申请的一部分实施例，而不是全部的实施例。基于本申请中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本申请保护的范围。
需要说明，若本申请实施例中有涉及方向性指示(诸如上、下、左、右、前、后……)，则该方向性指示仅用于解释在某一特定姿态(如附图所示)下各部件之间的相对位置关系、运动情况等，如果该特定姿态发生改变时，则该方向性指示也相应地随之改变。
另外，若本申请实施例中有涉及“第一”、“第二”等的描述，则该“第一”、“第二”等的描述仅用于描述目的，而不能理解为指示或暗示其相对重要性或者隐含指明所指示的技术特征的数量。由此，限定有“第一”、“第二”的特征可以明示或者隐含地包括至少一个该特征。另外，各个实施例之间的技术方案可以相互结合，但是必须是以本领域普通技术人员能够实现为基础，当技术方案的结合出现相互矛盾或无法实现时应当认为这种技术方案的结合不存在，也不在本申请要求的保护范围之内。
本申请提供一种POCT血液细胞分析仪及其使用方法。
如图1至图14所示，POCT血液细胞分析仪包括壳体10、显示屏11、出仓口12、注射器220、机架301、建压系统、检测座300及其传动机构、移液器400及其传动机构、金属屏蔽盖500及其传动机构等。
检测座300能够往复进出于出仓口12以便于试剂盒20装入或取出，移液器400及其传动机构用于实现待测样本(可以预装在试剂盒20上)的全自动化前处理，全自动化前处理包括自动加样本、自动加试剂(试剂盒20上预装有稀释液、溶血剂、染色裂解试剂等)、自动混匀(打气泡或搅拌)等，建压系统用于提供移液器400进行全自动化前处理所需的正压和负压，金属屏蔽盖500及其传动机构用于配合建压系统进行自动检测，自动检测是指金属屏蔽盖500与检测座300相盖合形成电磁屏蔽空间时，建压系统的气压连接装置510与试剂盒20上的压力作用腔140(压力作用腔140与阻抗检测池的后池相连通)连通，建压系统的压力室210开始提供负压或正压使得试剂盒20的阻抗检测池的前池120中的液体通过微孔流向试剂盒20的阻抗检测 池的后池并在此过程中记录相关参数。其中，建压系统的气压连接装置510与金属屏蔽盖500相连接并同步运动。
建压系统包括压力室210、联动设置的第一注射器222和第二注射器223、驱动件224、空气过滤器225、第一电磁阀SV1、第二电磁阀SV2、第三电磁阀SV3、气压连接装置510等。
移液器400包括导气管401、套设于导气管401外周的外套管402、同步块403、挡光片404、光耦405、固定板406、通槽407、用于卸载安装头(201或204)的阻拦件4031等。
金属屏蔽盖500通过安装架520安装在机架301上，并通过电机530、导柱540在竖直方向上进行升降运动，金属屏蔽盖500呈下部开口的罩体状，建压系统连接的气压连接装置510伸入至金属屏蔽盖500内部。
检测座300包括本体部310、与本体部310相连接的导电托303、包覆本体部310的金属屏蔽座(320、321、322)、嵌入本体部310的光学检测组件330、依次靠近光学检测组件330的检测腔设置的帕尔贴340、散热器350以及风扇360等。
以下将根据POCT血液细胞分析仪的各实施方式列举实施例具体说明。
第一实施例，如图1至图13所示，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括壳体10、检测座300、移液器400。
检测座300可伸入或伸出壳体10以用于接收设有阻抗检测池的试剂盒20装入，检测座300设有配合阻抗检测的供电件(例如图9中所示的导电托303)。试剂盒20具有安装头容置池，安装头容置池用于装设安装头(tip头201或穿刺头204)。
移液器400设于壳体10内并位于检测座300上方，用于对试剂盒20进行相应操作，相应操作包括移液操作、打气泡混匀操作、搅拌混匀操作以及刺破密封膜的操作。安装头需要卸载时(包括正常检测完成和意外断电两种情况)，移液器400运动至安装头容置池以外的预设位置(例如图12中的前池120)执行安装头卸载操作可以在意外断电重启的情况下确保移液器400不会在安装头容置池已经装有安装头的情况下重复放置安装头，还可以缩短卸载安装头时的运行路径，同时降低卸载安装头时的对准要求(一般安装头容置池的口径相对较小，卸载时需要精确对准)。
本实施例还提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括壳体10、检测座300、移液器400和检测器(可以是光耦405等检测元件)。
检测座300可伸入或伸出壳体10以用于接收设有阻抗检测池的试剂盒20装入，检测座300设有配合阻抗检测的供电件。试剂盒20具有安装头容置池，安装头容置池用于装设安装头。
移液器400设于壳体10内并位于检测座300上方，用于对试剂盒20进行相应操作。
检测器用于在POCT血细胞分析仪需要将安装头卸载时检测移液器400上是否加载有安装头，若检测到移液器400上加载有安装头时，则移液器400运动至安装头容置池或以外的预设位置执行安装头卸载操作，通过设置检测器，可以获知移液器400上是否加载有安装头进而选择性进行卸载操作，相对更为智能化。
本实施例还提供一种POCT血细胞分析仪，该POCT血细胞分析仪用于接收试剂盒20装入，该POCT血细胞分析仪包括检测座300和移液器400。
检测座300用于接收设有阻抗检测池的试剂盒20装入，检测座300设有配合阻抗检测的供电件。试剂盒20具有安装头容置池，安装头容置池用于装设安装头。
移液器400设于检测座300上方，用于加载安装头以对试剂盒20进行相应操作，安装头需要卸载时，移液器400运动至安装头容置池或安装头容置池以外的预设位置执行安装头卸载操作。本实施例中，该POCT血细胞分析仪还包括检测器(可以是光耦405等检测元件)，检测器用于在POCT血细胞分析仪需要将安装头卸载时检测移液器400上是否加载有安装头，若检测到移液器400上加载有安装头时，则移液器400将安装头卸载至安装头容置池或安装头容置池以外的预设位置。移液器400将安装头卸载至安装头容置池以外的预设位置操作上更为简单，即直接就近找大口径的池体进行卸载，可以避免在安装头容置池已经装有安装头的情况下重复放置安装头，还可以缩短卸载安装头时的运行路径，同时降低卸载安装头时的对准要求。
其中，试剂盒20具有多个池体，预设位置为其中的一个池体。多个池体包括与安装头匹配的安装头容置池和若干功能池，移液器400将安装头卸载至任一功能池中。功能池可包括稀释液池111、前池120(WBC检测池或RBC检测池)等，移液器400将安装头卸载至任一功能池中实际上是选择了一个绝对的空位和开口相对较大的池位。
如图6所示，移液器400包括导气管401和外套管402，外套管402相对导气管401作轴向运动时可将安装头卸载。
本实施例还提供一种POCT血细胞分析仪的使用方法，该使用方法包括：
POCT血细胞分析仪的移液器400运动至安装头容置池加载安装头以进行第一次检测的相关操作；
第一次检测存在正常检测完成和意外断电两种情况，在要进行第二次检测前，第一次检测时所使用的安装头需要卸载，POCT血细胞分析仪的移液器400运动至安装头容置池以外的预设位置执行安装头卸载操作。该使用方法可以确保移液器400不会在安装头容置池已经装有安装头的情况下重复放置安装头，还可以缩短卸载安装头时的运行路径，同时降低卸载安装头时的对准要求。
本实施例还提供一种POCT血细胞分析仪的使用方法，该使用方法包括：
POCT血细胞分析仪的移液器400运动至安装头容置池加载安装头以进行第一次检测的相关操作；
第一次检测存在正常检测完成和意外断电两种情况，在要进行第二次检测前，第一次检测时所使用的安装头需要卸载，可通过检测器检测POCT血细胞分析仪的移液器400上是否加载有安装头；
若检测到移液器400上加载有安装头时，移液器400运动至安装头容置池以外的预设位置执行安装头卸载操作。
该使用方法可以获知移液器400上是否加载有安装头进而选择性进行卸载操作，相对更为智能化。
本实施例还提供一种POCT血细胞分析仪的使用方法，包括：
POCT血细胞分析仪的移液器运动至安装头容置池加载安装头以进行第一次检测的相关操作；
第一次检测存在正常检测完成和意外断电两种情况，在要进行第二次检测前，第一次检测时所使用的试剂盒20、安装头需要取出，因此要检测POCT血细胞分析仪是否装设有试剂盒20；
在要进行第二次检测前，可通过检测器检测POCT血细胞分析仪的移液器400上是否加载有安装头；
若检测到POCT血细胞分析仪装设有试剂盒20且移液器400上加载有安装头时，则控制移液器400运动至安装头容置池或安装头容置池以外的预设位置执行安装头卸载操作。选择安装头容置池以外的预设位置执行安装头卸载操作时，不会在安装头容置池已经装有安装头的情况下重复放置安装头，还可以缩短卸载安装头时的运行路径，同时降低卸载安装头时的对准要求。
预设位置可对应于检测座300内部或对应于检测座300外部。也就是说，卸载操作不限于将安装头卸载至检测座300上或 卸载至试剂盒20上，还可以是直卸载至仪器内部除检测座300以外的区域，例如仪器内部设有一个安装头回收盒，维护人员定期清空安装头回收盒。
移液器400的外套管402相对移液器400的导气管401作轴向运动可将安装头卸载。在具体实施例中，移液器400通过固定设置的阻拦件或随动设置的可伸缩阻拦件4031将安装头卸载，阻拦件4031为伸缩电机、竖直或水平设置的电磁铁或U型口挡板，其中，外套管402上可连接有同步块403，阻拦件4031挡住同步块403且移液器400上升时，安装头被外套管402的下端卸载。
第二实施例，请一并参阅图1至图13，本实施例提供一种移液器400，该移液器400包括导气管401和检测件。
导气管401用于加载安装头(tip头201或穿刺头204)，检测件设于导气管401的侧边，用于检测导气管401上是加载接有安装头。
移液器400还包括外套管402，外套管402套设在导气管401的外周，在导气管401加载安装头时，导气管401插入安装头，以使得安装头推动外套管402沿导气管401的轴向产生位置变化，检测件用于检测外套管402的位置进而判断导气管401上是否套接有安装头。
导气管401上套接有安装头时外套管402位于导气管401的第一位置(图6所示意出的状态位置)，导气管401上未套接安装头时外套管402位于导气管401的第二位置，第一位置高于第二位置。
外套管402上连接有挡光片404，检测件为与挡光片404配套设置的光耦405，光耦405的光通道被挡光片404阻挡时，说明导气管401上套接有安装头，光耦405的光通道未被挡光片404阻挡时，说明导气管401上未套接有安装头。
移液器400包括设于导气管401侧边的固定板406，光耦设于固定板406上。其中，移液器400的导气管401单独可以进行上下运动，移液器400整体可以进行二维或三维运动。
固定板406设有通槽407，光耦405的一对光收发部伸入通孔并位于导气管401侧边，外套管402上还连接有同步块403，同步块403伸入通槽407，导气管401上升且同步块403受阻时安装头进行卸载，安装头卸载后，外套管402仍套接在导气管401外周，且由于同步块403伸入通槽407，外套管402相对导气管401并不会脱落，而是自然承托在通槽407的底部(也即第二位置)。
本实施例还提供一种POCT血细胞分析仪的使用方法，包括以下步骤：
在进行移液前判断检测移液器400的导气管401上是否装有安装头；
若检测到移液器400的导气管401上未装安装头，则发出警报。由于POCT血细胞分析仪的管路中均为气流，而非液流，管路中不能吸液体，因此导气管401必须在套接了安装头(tip头201)时才能进行相应操作，故需检测移液器400的导气管401上是否装有安装头。
检测移液器400的导气管401上是否装有安装头的步骤中，导气管401的外周套设有外套管402，通过检测件检测外套管402的位置进而判断导气管401上是否套接有安装头。
导气管401上套接有安装头时外套管402位于导气管401的第一位置，导气管401上未套接安装头时外套管402位于导气管401的第二位置，第一位置高于第二位置。
检测件为光耦405，外套管402上对应设有与光耦405配套的挡光片404。挡光片404挡住光耦405的光通道时判断为导气管401上套接有安装头外套管402。
本实施例提供一种POCT血细胞分析仪，包括壳体10、检测座300、移液器400、检测件。
检测座300可伸入或伸出壳体10，用于接收设有阻抗检测池的试剂盒20装入；移液器400设于壳体10内并位于检测座300上方，移液器400包括导气管401，导气管401用于加载安装头(tip头201或穿刺头204)；检测件设于导气管401的侧边，用于检测导气管401上是否加载有安装头，检测件可以是光耦405等可以进行物体有无的检测元件。
本实施例中，设置检测件可以获知安装头的加载状态，避免移液器在未加载安装头的状态下进行移液操作而污染POCT血细胞分析仪的内部气路。
第三实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300、设于检测座300上方的移液器400以及建压系统，检测座300用于接收试剂盒20装入，试剂盒20设有通过微孔连通的前池120和后池，建压系统包括压力室210、气压连接装置510、第一注射器222、第二注射器223、驱动件224。
第一注射器222与压力室210或移液器400连通，用于对压力室210建立正压或负压，还用于辅助移液器400进行移液，或者用于对前池120中的液体进行打气泡混匀，第二注射器223与与移液器400连通，用于吸吐样本、试剂，驱动件224用于同时驱动第一注射器222和第二注射器223，第一注射器222和第二注射器223可为联动式注射器，方便共用同一驱动件224，驱动件224可以是电机，当然，第一注射器222和第二注射器223也可以是分体的注射器并采用不同的电机独立控制。
POCT血细胞分析仪还包括电磁阀，电磁阀包括第一电磁阀SV1、第二电磁阀SV2、第三电磁阀SV3。
第一电磁阀SV1将第一注射器222与压力室210、第二注射器223选择性连通；第二电磁阀SV2将第一电磁阀SV1与压力室210、外界大气选择性连通，外界大气通过空气过滤器225、管路T10连接到第二电磁阀SV2；第三电磁阀SV3将气压连接装置510与压力室210选择性连通，第三电磁阀SV3通电时管路T8与T7经第三电磁阀SV3连通。
第一注射器222可通过空气过滤器225、T10管路、第二电磁阀SV2、T5管路、第一电磁阀SV1、T1管路吸气，再通过T1管路、第一电磁阀SV1、T5管路、第二电磁阀SV2、T6管路向压力室210建立正压。
第一注射器222可通过T1管路、第一电磁阀SV1、T5管路、第二电磁阀SV2、T6管路吸走压力室210中的气体以建立负压，再通过T1管路、第一电磁阀SV1、T5管路、第二电磁阀SV2、T10管路、空气过滤器225排气。
压力室210建立正压或负压后可通过T7管路、第三电磁阀SV3、T8管路、气压连接装置510输出正压或负压。其中，T8管路可采用相对较硬的管路，即T8管路的硬度大于第二导管221，T8管路的硬度较大可以避免在输出负压时T8管路的管壁贴合在一起阻塞T8管路的内部气流通道。
当第一电磁阀SV1将T1管路与T2管路连通时，第一注射器222和第二注射器223所产生的正压或负压同时作用到移液器400，即在需要大量吸样或推样时，第一注射器222可以起到辅助移液的作用。
第一注射器222的容积大于第二注射器223的容积，第一注射器222主要用于进行建压，第二注射器223主要用于推样/吸样，当然两者也可以根据需要同时使用进行建压或者推样/吸样。
第一注射器222的容积与第二注射器223的容积之比可为80～120：1，例如，第一注射器222的容积为10毫升，第二注射 器223的容积为100微升。驱动件224通过联动板231驱动第一注射器222与第二注射器223的活塞杆使得第一注射器222和第二注射器223同步运动。其中，第二注射器223的活塞杆与第二注射器223的外筒的尾端密封配合。
POCT血细胞分析仪还包括壳体10和机架301，压力室210设于壳体10上或设于机架301上，移液器400、第一注射器222、第二注射器223、驱动件设于机架301上。
检测座300相对机架301滑动或旋转设置并位于移液器400下方。
POCT血细胞分析仪还包括金属屏蔽盖500，检测座300相对机架301能够运动到配液工位(即图3中所示的位置)以便于移液器400进行相应操作，检测座300相对机架301能够运动到样本检测工位(图中未示出，对应于金属屏蔽盖500的正下方)，金属屏蔽盖500用于在样本检测工位处下降与检测座300相配合以形成一个相对封闭的金属腔体进而屏蔽外界电磁干扰。机架301可以构成双层结构，下层设置检测座300，上层设置移液器400、金属屏蔽盖500以及注射器220。
如图1所示，壳体10设有显示屏11和出仓口12，检测座300用于运动至出仓口12以接收试剂盒20装入检测座300，配液工位与出仓口12的距离小于样本检测工位与出仓口12的距离，也就是说，出仓口12朝向仪器内部依次为配液工位和样本检测工位。检测座300往复运动于装载工位、配液工位和样本检测工位之间，配液工位位于装载工位和样本检测工位之间。在其它实施中，配液工位和样本检测工位也可以是同一工位。
本实施例还提供一种基于前述的POCT血细胞分析仪的使用方法，包括以步骤：
控制驱动件224运动以通过第一注射器222对压力室210建立正压或负压；
控制驱动件224运动以通过第二注射器223和移液器400进行移液；
控制压力室210的压力释放以通过气压连接装置510向前池120施加正压或向后池施加负压实现前池120中的液体经微孔流向后池。
本实施例提供的POCT血细胞分析仪布局合理，结构紧凑，使用方便。
第四实施例，请一并参阅图1至图13，本实施例提供一种试剂盒20，该试剂盒20包括盒体100和微流控检测片600。
其中，盒体100包括用于进行阻抗法检测的阻抗检测池(包含相连通的前池120、后池、压力作用腔140)。
微流控检测片600与盒体100一体连接、装配连接或不连接并位于阻抗检测池的侧边。
微流控检测片600设有加样孔601以接收样本进入后平铺于微流控检测片600内。
盒体100还包括稀释液池111、溶血剂池106、光学检测池、染色裂解池602，稀释液池111用于封装稀释液，溶血剂池106用于封装溶血剂，染色裂解池602用于封装染色裂解试剂，光学检测池与盒体100一体连接或装配连接。
盒体100还包括安装头容置池和样本容置池，安装头容置池用于放置安装头(tip头201或)穿刺头204，样本容置池用于放置样本管。
请一并参阅图13，本实施例还提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300、图像识别底座610、图像识别装置620。
其中，检测座300设有配合阻抗检测的供电件(例如图9中所示的导电托303)，检测座300用于接收前述的试剂盒20装入，图像识别底座610设于检测座300侧边，图像识别装置620设于图像识别底座610上方，用于与图像识别底座610配合以对微流控检测片600进行图像检测。
POCT血细胞分析仪还包括移液器400，移液器400设于检测座300上方，该POCT血细胞分析仪包括与检测座300匹配的金属屏蔽盖500，金属屏蔽盖500能够进行升降运动或旋转运动，图像识别装置620独立设置或者与金属屏蔽盖500连接。
本实施例提供的POCT血细胞分析仪及试剂盒20能够同时支持阻抗检测和微流控图像检测，联检效率优化，效果较好。
第五实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括机架301、检测座300、移液器400。
检测座300相对机架301滑动设置或旋转设置，检测座300往复运动于装载工位及样本检测工位，检测座300滑出或旋出机架301时位于装载工位以接收试剂盒20装入或者允许试剂盒20取出。
移液器400设于壳体10内并位于检测座300上方，用于对试剂盒20进行相应操作。
POCT血细胞分析仪还包括配液工位；配液工位于装载工位和样本检测工位之间；或者配液工位与样本检测工位为同一工位。
检测座300呈上部开口的半包围状，检测座300与试剂盒20卡扣配合，检测座300包括本体部310及包覆于本体部310外周的金属屏蔽座(320、321、322)。
POCT血细胞分析仪还包括与金属屏蔽座(320、321、322)匹配的金属屏蔽盖500。金属屏蔽盖500设于检测座300上方并在样本检测工位配置为能够在竖直方向上运动，金属屏蔽盖500的竖直边缘与金属屏蔽座(320)的上表面相抵接。
POCT血细胞分析仪还包括建压系统，建压系统的一端设有伸入金属屏蔽盖500并随金属屏蔽盖500同步运动的气压连接装置510，试剂盒20设有与气压连接装置510对接的压力作用腔140。
本实施例提供一种POCT血细胞分析仪的使用方法，该方法包括以下步骤：
检测座300滑出或旋出机架301以在装载工位接收试剂盒20装入；
检测座300滑入或旋入机架301并运动到配液工位；
移液器400运动以加载试剂盒20上预放的安装头(tip头201或穿刺头204)进行相应操作以完成配液；
检测座300由配液工位运动至金属屏蔽盖500下方的样本检测工位；
金属屏蔽盖500向下运动与检测座300配合；
金属屏蔽盖500上连接的气压连接装置510开始提供压力，将试剂盒20上的阻抗检测池中的液体进行引流进而进行阻抗法检测。
本实施例提供的POCT血细胞分析仪及其使用方法自动化程度高，待测液的前处理配液操作均在仪器的内部自动完成，避免了人工操作的随机性和误差性。
第六实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300和金属屏蔽盖500。
检测座300往复运动于装载工位及样本检测工位，用于在装载工位接收设有阻抗检测池的试剂盒20装入，金属屏蔽盖500设于样本检测工位处，用于在检测座300运动至样本检测工位时与检测座300相盖合以进行电磁信号屏蔽。POCT血细胞分析仪还包括配液工位；配液工位位于装载工位和样本检测工位之间；或者配液工位与样本检测工位为同一工位。
检测座300呈上部开口的半包围状，金属屏蔽盖500呈下部开口的半包围状，检测座300与试剂盒20卡扣配合，检测座300包括本体部310及包覆于本体部310外周的金属屏蔽座(320、321、322)，检测座300设置成能够在金属屏蔽盖500下方平移或旋转，金属屏蔽盖500设置成能够在竖直方向上上下运动。
POCT血细胞分析仪还包括建压系统，建压系统的一端设有伸入金属屏蔽盖500并随金属屏蔽盖500同步运动的气压连接装置510，试剂盒20设有与气压连接装置510对接的压力作用腔140。
本实施例还提供一种POCT血细胞分析仪的使用方法，该方法包括以下步骤：
检测座300滑出或旋出机架301以在装载工位接收试剂盒20装入；
检测座300滑入或旋入机架301并运动到配液工位；
移液器400于配液工位处对试剂盒20进行相应操作；
金属屏蔽盖500与检测座300相盖合并进行阻抗法检测，具体地，检测座300先运动至金属屏蔽盖500下方，然后金属屏蔽盖500向下运动与检测座300相盖合。
其中，移液器400对试剂盒20进行配液包括：移液器400吸取试剂盒20中预装的血样和试剂并移入试剂盒20的检测池中，其中试剂包括溶血剂和稀释液等。
本实施例提供的POCT血细胞分析仪及其使用方法自动化程度高，待测液的前处理配液操作均在仪器的内部自动完成，避免了人工操作的随机性和误差性。
第七实施例，请一并参阅图1至图13，本实施例提供一种检测座300，该检测座300包括检测腔302和导电托303。
检测腔302用于接收设有阻抗检测池的试剂盒20装入，试剂盒20设有电极，导电托303用于电连接电极。电极为设于试剂盒20相对两侧的导电柱122。
如图10所示，在一实施例中，导电托303包括向下倾斜的弹片部304，弹片部304用于承托并电连接导电柱122。导电托303还包括与弹片部304一体连接的固定连接部305，固定连接部305的形状不作限定，其可以设置固定孔以便于通过螺钉进行紧固装配。
如图11所示，在另一实施例中，导电托303包括一体连接且呈竖向设置的固定连接部305和弯折延伸的折弯部306(可以是V型折弯或者圆弧形折弯)，导电柱122由上往下装配时挤开折弯部306，折弯部306在导电柱122装配到位时复位并与导电柱122相抵接，该形式的导电托303弹性形变能力较好，经久耐用，能够长期保持可靠的电性连接。该导电托303可以是一个并设置在导电柱122的一侧，也可以是两个且分别设置在导电柱122的两侧以进一步增加电性连接的可靠性。具体地，折弯部306可包括依次与固定连接部305的第一折弯部3061和第二折弯部3062，导电柱122由上往下装配时挤开第一折弯部3061，第二折弯部3062在导电柱122装配到位时与导电柱122相抵接。
检测座300还包括本体部310和金属屏蔽座(320、321、322)，本体部310设有检测腔302，本体部310的侧壁设有与检测腔302连通的装配槽307，导电托303经装配槽307伸入检测腔302，金属屏蔽座(320、321、322)罩设于本体部310外周，并与导电托303绝缘间隔，金属屏蔽座(320、321、322)包括围设于本体部310周围的侧板(320、321)和贴合于本体部310底部的底板(322)。
试剂盒20还包括一体连接或者可拆卸的光学检测池，可拆卸的光学检测池可通过矩形插装孔107插装，检测座300还包括与光学检测池对应的光学检测组件330，光学检测组件330包括间隔设置的发光组件、收光组件以及位于发光组件和收光组件之间检测腔，发光组件的轴线与收光组件的轴线的夹角范围是0至60度，具体可以是0度、20度、30度、45度、60度等。
光学检测组件330为多组，多组光学检测组件330的光源腔通过挡光围壁333相互间隔以避免检测光相互干扰。
例如，多组光学检测组件330可包括两组激光检测组件331和一组LED光源检测组件332，激光检测组件331的检测腔之间设有挡光围壁333，挡光围壁333优选作发黑处理，以使其吸光效果较好且反光效果较差，避免反射光影响正常的光学检测。
检测座300还包括靠近激光检测组件331的检测腔依次设置的帕尔贴340、散热器350以及风扇360，散热器350包括与帕尔贴340贴合的吸热基板351及与吸热基板351垂直连接的散热鳍片352，风扇360可设于散热鳍片352远离吸热基板351的一端，通过设置帕尔贴340、散热器350以及风扇360可以控制检测温度，在预设的检测温度下进行检测所获得的检测结果相对更为精准。
检测腔302的底部设有轻触开关334，轻触开关334可以获知试剂盒20的装载情况，当装有试剂盒20时，轻触开关334被按下，未装有试剂盒20时，轻触开关334处于弹起状态。
本实施例还提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括移液器400和前述的检测座300及设于检测座300上方的移液器400，移液器400用于对试剂盒20进行相应操作。
本实施例提供的POCT血细胞分析仪及其检测座300的导电托303包括向下倾斜的弹片部304，在与试剂盒20的电极进行电连接配合时具有较好的适配性和稳定性，极大的降低了接触不良的可能性。
第八实施例，本实施例提供一种POCT血细胞分析仪的使用方法，包括以步骤：
响应于试剂盒20放入检测座300，控制移液器400移动至试剂盒20的第一安装头容置池上方并下降以加载第一安装头，其中第一安装头可为tip头201；
控制移液器400移动至试剂盒20的稀释液池111将稀释液移入用于进行阻抗法检测的前池120(即WBC检测池和/或RBC检测池)；
控制移液器400移动至样本管/样本稀释池/其它样本放置处将样本移入前池120，其中样本稀释池中的样本为稀释后的样本；
对前池120中的待测液进行打气泡混匀或搅拌；
控制金属屏蔽盖500与检测座300相盖合，以在试剂盒20的后池与建压系统之间建立起连通关系；
控制建压系统将待测液从前池120引流入后池；
通过阻抗检测法对待测液进行检测。
其中，控制移液器400移动至试剂盒20的第一安装头容置池上方并下降以加载第一安装头包括：控制移液器400相对第一安装头放置池作三维移动，使移液器400与第一安装头相互挤压而加载上第一安装头。
控制移液器400移动至试剂盒20的稀释液池111将稀释池移入用于进行阻抗法检测的前池120和/或控制移液器400移动至样本管/样本稀释池/其它样本放置处将样本移入前池120包括：移液器400相对前池120作三维运动并利用第一安装头的内腔作为液体中转位吸取稀释液和/或样本，第一安装头的内腔的容积一般为100微升至1000微升，并且使用时一般不会超过该容积的 4/5以避免液体进入建压系统的管路形成污染。
对前池120中的待测液进行混匀包括移液器400通过第一安装头吸吐待测液；或者移液器400通过第一安装头向待测液中打入气泡；或者移液器400通过第一安装头对待测液进行搅拌。
对前池120中的待测液进行混匀之后还包括移液器400将第一安装头卸载至第一安装头容置池。
移液器400将第一安装头卸载至第一安装头容置池之后还包括移液器400移动至第二安装头容置池上方并下降以加载第二安装头。
移液器400移动至第二安装头容置池并下降以加载第二安装头之后包括：
移液器400移动至样本管/样本稀释池/其它样本放置处将样本移入光检测池；
通过光学检测法对待测液进行检测。
移液器400移动至第二安装头容置池并下降以加载第二安装头之后包括：
移液器400移动至样本管将样本移入染色裂解池602孵育后再移入微流控检测片600；
通过图像检测法对微流控检测片600中的粒子进行检测。
本实施例还提供一种POCT血细胞分析仪，POCT血细胞分析仪包括检测座300、移液器400、建压系统、金属屏蔽盖500、传动组件。
检测座300用于接收试剂盒20装入，试剂盒20上设有通过微孔连通的前池120和后池并装设有第一安装头、稀释液、样本；
移液器400用于加载第一安装头并将稀释液、样本移入前池120；
金属屏蔽盖500用于与检测座300相盖合，建压系统用于将待测液从前池120引流入后池。
传动组件用于驱动检测座300、移液器400以及金属屏蔽盖500进行一维、二维或三维运动，传动组件可通过电机、丝杆、丝母、滑轨、滑块、齿轮、齿条、同步带等构成。
试剂盒20上还装设有第二安装头、光学检测池，移液器400还用于加载第二安装头并将样本移入光学检测池以进行光学检测。
或者，试剂盒20上还装设有第二安装头、染色裂解试剂、微流控检测片600，POCT血细胞分析仪还包括间隔设置的图像检测底座610和图像检测装置620，移液器400还用于加载第二安装头并将样本移入染色裂解试剂中孵育，再将孵育后的样本移入微流控检测片600，并通过图像检测底座610和图像检测装置620对微流控检测片600进行图像检测。
本实施例提供的POCT血细胞分析仪及其使用方法自动化程度高，在仪器内部完成自动化配液，过程操作一致性较好，能够同时支持阻抗检测、光学检测、图像检测。
第九实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300、穿刺头加载机构、穿刺头204、移液器400。其中穿刺头加载机构可以是独立的机构，也可以和移液器400是同一机构。
检测座300用于装入试剂盒20，试剂盒20上设置有稀释液池和溶血剂池，稀释液池和溶血剂池的开口设密封膜。
穿刺头204用于对装入POCT血细胞分析仪的试剂盒20上的至少两处密封膜进行逐一刺破，穿刺头加载机构用于加载穿刺头204。
穿刺头204设于试剂盒20上和/或检测座300上。试剂盒20和/或检测座300上设有与穿刺头204匹配的放置位，穿刺头204通过移液器400加载后随移液器400进行同步运动。
穿刺头204穿过密封膜后进一步水平摆动，由此可以扩大密封膜上所形成的通孔口径。
优选的，至少两处密封膜位于同一直线上或同一弧线上，由此可以使得逐一刺破的动作轨迹相对简单。
如图7所示，穿刺头204包括主体部2042和尖锐部2041，尖锐部2041设于主体部2042的一端。尖锐部2041的截面可呈一字型、十字型、米字型或Y型等。主体部2042远离尖锐部2041的一端设有法兰部2044，法兰部2044可以方便穿刺头204挂装在穿刺头容置池104，还可以与外套管402形成稳定的抵接配合。
第十实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300和安装头。
检测座300用于装入试剂盒20，试剂盒20上设置有稀释液池和溶血剂池，稀释液池和溶血剂池的开口设密封膜，安装头用于对装入POCT血细胞分析仪的试剂盒20上的密封膜进行逐一刺破。本实施例中，安装头是tip头201或专用的穿刺头204，安装头设于试剂盒20上。试剂盒20上设有与安装头匹配的放置位，穿刺头204的外径大于tip头201的外径，穿刺头204穿过密封膜后形成的开口大于tip头201伸入开口处的直径，由此可以在tip头吸液时防止池内形成负压腔，保证吸液时的精准度。
POCT血细胞分析仪还包括移液器400，安装头通过移液器400加载后随移液器400进行运动。
至少两处密封膜位于同一直线上或同一弧线上。由此可以使得逐一刺破的动作轨迹相对简单。安装头穿过密封膜后进一步水平摆动以扩大密封膜上的通孔的口径。
在一实施例中，tip头201可先以第一行程刺第一通孔，再以第二行程刺第二通孔以进行吸样，第一行程小于第二行程，由此可以避免吸液时形成负压密封腔影响吸液时的精准度。
在另一实施例中，tip头刺完通孔后进行抬升再进行吸样，同样可以避免吸液时形成负压密封腔影响吸液时的精准度。
其中，穿刺头204包括主体部2042和设于主体部2042端部的尖锐部2041。
尖锐部2041的截面呈一字型、十字型、米字型或Y型等。主体部2042部远离尖锐部2041的一端设有法兰部2044。
本实施例中，可以直接采用tip头201进行穿刺，也可以采用专用的穿刺头204进行穿刺。
第十一实施例，请一并参阅图1至图13，本实施例提供一种用于POCT血细胞分析仪，其包括壳体10、移液器400、穿刺头204。
移液器400设于壳体10内，移液器400包括导气管401，穿刺头204包括主体部2042和尖锐部2041，尖锐部2041设于主体部2042的一端，主体部2042的另一端设置有供导气管401插入的容置腔2045。
尖锐部2041用于在POCT血细胞分析仪进行自动化检测时刺破试剂盒20上的密封膜。尖锐部2041的截面呈一字型、十字型、米字型或Y形，主体部2042远离尖锐部2041的一端设有法兰部2044以便于在试剂盒20的盒体100上挂装。POCT血细胞分析仪还包括检测座300，检测座300用于装设试剂盒20，试剂盒20设有穿刺头放置池，法兰部2044承托于穿刺头放置池外周。
移液器还包括套设于导气管401外周的外套管402，外套管402的下端与法兰部2042相抵接，主体部2042靠近法兰部2044的一端设有多个朝向尖锐部2041延伸的筋条2043以提高强度。穿刺头204可采用金属或塑胶材料一体成型。
如图12所示，本实施例提供一种试剂盒20，试剂盒20包括多个池位，其中一个池位用于放置前述的穿刺头204。
多个池位包括稀释液池111和/或溶血剂池106，稀释液池111和/或溶血剂池106设有密封膜(图未示)。
池位还包括用于进行阻抗法检测的WBC检测池和/或RBC检测池。
如图12所示，试剂盒20还包括微流控检测片600，微流控检测片600可以通过图像检测底座610、图像检测装置620进行图像检测。
本实施例中，密封膜的刺破操作可以无需通过人工操作，采用专用的穿刺头204或tip头201可以实现较好的一致性操作。
第十二实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300和建压组件。
检测座300用于装设试剂盒20，试剂盒20包括用于进行阻抗法检测的阻抗检测池，阻抗检测池包括通过微孔连通的前池120和后池，建压组件与前池120和/或后池气路连通用于提供压力以使得前池120中的待测液经微孔流向后池，还用于在待测液注入前池120前向前池120或后池提供压力使得微孔畅通，建压组件提供负压时，可以进行阻抗法检测，建压组件提供正压时，可以反冲微孔以保证微孔的畅通性。
建压组件包括气路连通的注射器220和压力室210，注射器220用于为压力室210建立正压或负压。建压组件用于提供气流以对待测液实现气泡混匀或用于使得微孔畅通，具体可以是经前池120/后池吹气或吸气使得微孔畅通。
POCT血细胞分析仪包括多组选通阀，选通阀连接压力室210和注射器220。
POCT血细胞分析仪包括与检测座300配合的金属屏蔽盖500，金属屏蔽盖500上设有与压力室210或注射器220连通的气压连接装置510，气压连接装置510用于与后池或前池120连通。金属屏蔽盖500设置成在竖直方向运动。
POCT血细胞分析仪还包括移液器400，移液器400设置在检测座300上方，用于对试剂盒20进行相应操作。
移液器400通过管道与建压组件气路连通，移液器400用于加载试剂盒20上的安装头并通过安装头进行相应操作。
本实施例还提供一种POCT血细胞分析仪的使用方法，其包括以下步骤：
接收试剂盒装入检测座300，试剂盒包括用于进行阻抗法检测的阻抗检测池，阻抗检测池包括通过微孔连通的前池120和后池；
在待测液注入前池120前向前池120或后池提供压力使得微孔畅通，其具体可包括：通过建压组件的压力室210中的压力向前池120或后池提供压力使得微孔畅通；或者通过建压组件的注射器220向前池120或后池提供压力使得微孔畅通。
第十三实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括检测座300、人机交互模块以及处理器，检测座300用于接收试剂盒20装入，试剂盒20上设有至少两种项目检测池，检测座300内设置有对项目检测池所对应的项目进行检测的辅助检测器，人机交互模块与检测座300通信连接，用于选择检测项目，人机交互模块为触控屏或显示屏11与机械按键的结合，处理器与检测座300、人机交互模块信号连接，用于接收检测项目和辅助检测器的检测信号，并比对检测项目和检测信号是否相对应，若相对应则可正常进行后续操作，若不相对应，则需要更换正确的试剂盒20或者重新选择正确的检测项目。项目检测池以可拆卸方式加装于试剂盒20上，辅助检测器用于检测是否存在相应的项目检测池。
辅助检测器包括光学检测组件330，光学检测组件330包括发光组件和收光组件，收光组件获得的光信号用于辅助判断试剂盒20上的项目检测池与选择检测项目是否对应。
辅助检测器包括轻触开关334，轻触开关334获得的信号用于辅助判断试剂盒20上的项目检测池与选择检测项目是否对应。
辅助检测器包括间隔相对设置的图像识别底座610和图像识别装置620，图像识别底座610和图像识别装置620获得的信号用于辅助判断试剂盒20上的项目检测池与选择检测项目是否对应。
POCT血细胞分析仪还包括扫码器，扫码器用于获取试剂盒20对应的标签信息，标签信息可包括名称、类型、生产时间等信息。
本实施例还提供一种POCT血细胞分析仪的使用方法，包括以下步骤：
在人机交互模块选择要检测的项目；
获取试剂盒20相应的检测位信息；
通过处理器判断检测位信息是否与选择的要检测项目相对应，如果不对应则输出相应提示，若相对应则可正常进行后续操作，若不相对应，则需要更换正确的试剂盒20或者重新选择正确的检测项目。
获取试剂盒20相应的检测位信息的步骤中，试剂盒20相应的检测位信息的包括散射光信号、透射光信号或微动开关触发的电信号。
其中，要检测的项目包括血常规检测、CRP检测、SAA检测、血细胞分类检测中的至少一种。
本实施例还提供一种POCT血细胞分析仪的使用方法，包括：
通过辅助检测器获取试剂盒20支持的项目检测信息；
在人机交互模块选择要检测的项目；
判断试剂盒20支持支持的项目检测信息是否与选择的要检测项目相对应，如果不对应则输出相应提示。
本实施例提供的方案在检测前对用户想要检测的项目和试剂盒实际能够支持的检测的项目进行比对，能够较好的起到防呆效果。
第一十四实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括壳体10、检测座300、移液器400、第一导管211以及防折件212。
检测座300可伸入或伸出壳体10，用于接收设有阻抗检测池的试剂盒20装入，检测座300设有配合阻抗检测的供电件(例如图9中所示的导电托303)。
移液器400设于壳体10内并位于检测座300上方，用于对试剂盒20进行相应操作。
第一导管211的一端与移液器400气路连通，第一导管211的另一端与建压系统(例如图5中的第二注射器223)连通，建压系统可包括注射器220、压力室210、电磁阀(SV1、SV2、SV3)、多通接头、汇流板中的至少一个，防折件212与第一导管211相结合以用于防止第一导管211过度弯折而阻塞第一导管211的内部导气通道。第一导管211的内径相对较细，一般可为0.5毫米至1.5毫米，在实际使用中容易出现过度弯折而阻塞的情况。
防折件212可为套设于第一导管211外周的套管，套管的硬度小于或等于第一导管211的硬度，当然，套管的硬度也可大于第一导管211的硬度，第一导管211的外周套设有套管后，二者的整体硬度相对增加，第一导管211的硬度相对较大，第一导管211中的气压变化时第一导管211本身的形变相对较小，进而可以提高吸样时的精度，套管的壁厚可大于或等于第一导管211的壁厚，套管的抗弯曲能力可小于或等于第一导管211的抗弯曲能力，由此可以减小套管对第一导管211的磨损，同时防止第一导管211过度弯折而阻塞第一导管211的内部导气通道。套管的截面为圆环形，或者套管的截面为C型。防折件212也可为套设于第一导管211外周的弹簧。防折件212还可为卷绕包覆在第一导管211外周的绑带，绑带可为布料绑带、塑胶绑带或金属绑带等。
第一导管211的两端分别连接移液器400和第二注射器223。第一导管211的端部设有接头部，接头部可以设置在第一导管211一端或两端，接头部具体可以是用于进行气路密封连接的卡箍、卡箍接头、翻边接头、直通接头或螺纹接头。
本实施例提供POCT血细胞分析仪由于在相对较细的第一导管211的外周设有防折件212，可以较好的避免出现气路不通的情况出现，提高仪器稳定性。
第十五实施例，请一并参阅图1至图13，本实施例提供一种POCT血细胞分析仪，该POCT血细胞分析仪包括壳体10、检测座300、移液器400、建压系统、第一导管211和第二导管221。
检测座300可伸入或伸出壳体10，用于接收设有阻抗检测池的试剂盒20装入，检测座300设有配合阻抗检测的供电件(例如图9中所示的导电托303)。
移液器400设于壳体10内并位于检测座300上方，用于对试剂盒20进行相应操作。
第一导管211的两端分别连接移液器400和第二注射器223，第二导管221将注射器220、压力室210、电磁阀(SV1、SV2、SV3)相连接，第一导管211(即图5中的T3管路)的内径小于或等于第二导管221(即图5中图除T3、T4以外的管路)的内径，第一导管211的硬度大于第二导管221的硬度。其中，第一导管211的作用主要是控制吸样或推样的精准度，要求气体压缩产生的误差较小，因而硬度大且内径相对较小，而第二导管221的作用主要是建压，要求快速，内径可相对较大。
第一导管211的内径与第二导管221的内径的比值可大于0.5且小于或等于1。第一导管211的长度与第二导管221的长度的比值大于1且小于或等于1.5，第一导管211相对较长可以便于安装接头(例如卡箍接头)。也就是说，第一导管211相对较细且较硬，第二导管221相对较粗且较软。
如图5所示，POCT血细胞分析仪还包括防折件212，防折件212与第一导管211相结合以用于防止第一导管211过度弯折而阻塞第一导管211的内部导气通道。
防折件212可为套设于第一导管211外周的套管或弹簧。套管的硬度小于或等于第一导管211的硬度，当然，套管的硬度也可大于第一导管211的硬度，第一导管211的外周套设有套管后，二者的整体硬度相对增加，第一导管211的硬度相对较大，第一导管211中的气压变化时第一导管211本身的形变相对较小，进而可以提高吸吐作业时的精度，套管的壁厚可大于或等于第一导管211的壁厚，套管的抗弯曲能力可小于或等于第一导管211的抗弯曲能力。套管的截面为圆环形或C型。防折件212也可为卷绕包覆在第一导管211外周的绑带。绑带为布料绑带、塑胶绑带或金属绑带。
第一导管211的硬度大于或等于第二导管221的硬度，第一导管211的硬度相对较大，第一导管211中的气压变化时第一导管211本身的形变相对较小，进而可以提高吸样时的精度；第一导管211的壁厚小于或等于第二导管221的壁厚；第一导管211的抗弯曲能力小于或等于第二导管221的抗弯曲能力。本实施例根据两种不同的需求对应进行内径、硬度选配，可以针对性的满足需求，实现仪器的精准、高效，并且针对较细的第一导管211设计有防折件212，可以较好的避免出现气路不通的情况出现，进一步提高了仪器稳定性。
第十六实施例，请一并参阅图1至图14本实施例提供一种POCT血细胞分析仪的使用方法，该方法可包括以下步骤：
S10，接收开机触发信号，具体可以是用户按下机械按键接通电源；
S11，进行硬件自检，即对电路板上各端口输出的各路电压、电流进行检测反馈；
S12，判断移液器是否加载有安装头，可以通过对射光耦或者反射光耦等检测元件进行判断，安装头可以是tip头或者穿刺头；
S18，若是，则移液器进行安装头卸载，卸载的位置可以是仪器内部的特定位置，也可以是仪器内部的试剂盒20上的某些池位；
S13，若否，则进行整机初始化，初始化即各个运动部件进行零位复位或者零位确认和压力清零，初始化完成后即可接收试剂盒20装入，并在POCT血细胞分析仪内进行全自动化样本前处理(自动化混合样本和相应的试剂)，在自动化样本前处理完成后进行样本检测。
其中，移液器进行安装头卸载之后包括：
S19，检测座300出仓以供试剂盒20取出，在此情况下，通常是上一次使用时意外断电，安装头和试剂盒20仍然遗留在仪器内部；
S20，检测座300回仓再进行整机初始化步骤，本步骤是为了确保机内无任何外部元件(安装头和试剂盒20)，然后便于后续的开机流程动作不会因为tip头或者试剂盒20的存在而受到影响。
判断移液器是否加载有安装头的步骤中，还包括：判断检测座300内是否安装有试剂盒20，一般来说，如果意外断电，仪器内部会同时存在试剂盒20和安装头，因此可以仅判断仪器内部是否有安装头，也可以同时判断仪器内部是否有安装头和是否有试剂盒20，其中，试剂盒20的判断可以通过检测座300内的轻触开关等方式判断。
进行整机初始化的步骤之后，还包括：
S14，进行压力检测，判断气路管路中的压力是否达到阈值范围，本申请提供的POCT血细胞分析仪内可设有一个、两个或者多个压力室210，并通过注射器建立正压或负压并达到一定的压力值范围，具体地，初始化之后进行压力自检流程，压力自检需要建压一定数值的负压，这里可建压-25～30kpa，建压过程依靠电磁阀、注射器、压力传感器、压力室210和气路管道组成。建压过程如下：建压过程中，压力传感器时刻检测着压力室210的压力，首先注射器电机以最大速度带动注射器往外拉动，同时打开电磁阀进行建立负压操作，此时注射器直接连通着压力室210，压力室210没有其它换气口打开，注射器往外拉时便可建立负压。如果注射器电机走到了最大行程，即注射器已经拉到最大量程之后，还未建到目标压力，则关闭电磁阀，此时注射器以最大速度用最大距离进行初始化，初始化完成后，再打开电磁阀同时继续外拉注射器建压，直到达到目标压力，如果建压工期结束还未达到目标压力则报建压故障。此时建压完成。压力自检继续，当目标压力建成后，压力传感器检测正常则打开电磁 阀，将压力室210的内部与空气连通，释放负压。压力自检后进行简单的空白测试，无需放置试剂盒20，直接测量空载情况下的信号确保POCT血细胞分析仪测试性能正常。接下来进行主测量过程。
S15，进行信号检测，包括判断电磁屏蔽组件配合后的检测信号进而判断电磁屏蔽效果以及判断电流、电压信号是否在阈值范围内，其中信号检测可包括各种光电信号等，还包括金属屏蔽盖和金属屏蔽座组合封闭后模拟进行阻抗法的检测，根据输出结果以判断金属屏蔽盖和金属屏蔽座的屏蔽效果或者电路板卡自身的信号质量。
S16，进入待机状态，此时可以选择关机或者选择进行检测。
具体地，进入待机状态的步骤之后包括：
S17，接收关机触发信号，即用户按下关机键，为了防止误操作，可以设置成用户按下关机键后，屏幕显示是否确定关机；
S21，判断试剂盒20有无，可通过检测座300内的轻触开关进行试剂盒20有无判断；
S22，若判断试剂盒20为有时进一步判断仓门状态；
S24，若判断仓门为打开状态，则提示取出试剂盒20并回到判断试剂盒20有无的步骤；
S23，若判断仓门为关闭状态，则打开仓门并提示取出试剂盒20再回到判断试剂盒20有无的步骤；
S25，若判断试剂盒20为无时进一步判断仓门状态；
S26，若判断仓门为打开状态，则关闭仓门并提示关闭电源；
S27，若判断仓门为关闭状态，则提示关闭电源。
进入待机状态的步骤之后包括：
S31，接收样本ID设置和测量模式设置，具体地，屏幕上可以提供模式设置等选项，提供一些可选的项目给用户进行选择，如CRP检测、SAA检测、血液检测等；
S32，接收启动测试触发信号，即屏幕上或者仪器上可以提供触控按键或实体按键以生成启动测试触发信号；
S33，通过弹窗提示核对待测信息(如试剂信息，或者扫码对比/读取IC卡对比)与测量模式是否匹配，若不匹配则点击取消(S46)并重新回到步骤S31，接收样本ID设置和测量模式设置；
S34，若匹配则点击确认；
S35，自动打开仓门；
S36，可通过弹窗等方式提示放入试剂盒；
S37，在放入试剂盒后点击确定；
S38，进行试剂盒20有无检测，若无则回到步骤S36，提示放入试剂盒20；
S39，若有则自动关闭仓门；
S40，进行自动化配液测试，自动化配液测试的具体流程可以参考前述实施例。
S41，显示测试结果，具体可以通过显示屏显示或者打印设备输出测试结果；
S42，点击确定；
S43，自动打开仓门并提示取出试剂盒20；
S44，用户取出试剂盒20后点击确定；
S45，自动关闭仓门并进入待机状态。
本实施例提供的POCT血细胞分析仪的使用方法合理有序，能够快速的进行单个项目检测或多个项目同时检测。
本实施例还提供一种POCT血细胞分析仪，包括壳体10、检测座300移液器400。
检测座300可伸入或伸出壳体10，用于接收试剂盒20装入，并往复运动于试剂盒20加载工位和样本检测工位之间；移液器400，设于壳体10内并位于检测座300上方，用于对试剂盒20进行自动化样本前处理。
试剂盒20包括通过微孔连通的前池120和后池，POCT血细胞分析仪还包括建压系统，建压系统设于壳体10内并用于使得待测液从前池120流向后池以进行阻抗检测法检测。
POCT血细胞分析仪还包括用于与检测座300相盖合的金属屏蔽座(320、321、322)，试剂盒20包括与后池连通的压力作用腔140，金属屏蔽座(320、321、322)设有与建压系统连接的气压连接装置510，金属屏蔽座(320、321、322)与检测座300相盖合时气压连接装置510与压力作用腔140密封对接。
移液器400上设有检测件(例如图6所示的光耦405)，移液器400包括导气管401和套设在导气管401的外周的外套管402，导气管401用于加载装设于试剂盒20上的安装头，在导气管401加载安装头时，导气管401插入安装头，以使得安装头推动外套管402沿导气管401的轴向产生位置变化，检测件用于检测外套管402的位置进而判断导气管401上是否套接有安装头。
移液器400还包括退头机构(参考前述的阻拦件4031)，退头机构用于在导气管401上升时止挡外套管402上升以使得外套管402将安装头卸载。
退头机构为相对导气管401的轴向或者径向设置的电磁铁、电机、或者U型口挡板。
建压系统包括压力室210、联动设置的第一注射器222和第二注射器223，第一注射器222用于为压力室210建立正压或负压，第二注射器223与移液器400连通，用于在移液器400套接有安装头时利用安装头的内腔作为液体中转位吸取试剂和/或样本。
POCT血细胞分析仪包括第一导管211和第二导管221，第一导管211连接于第二注射器223和移液器400之间，第二导管221用于连接注射器和压力室210，第一导管211的内径小于第二导管221的内径。
第一导管211的外周设有防折件212，防折件212为套管、弹簧或者绑带。
本实施例还提供一种POCT血细胞分析仪，其包括检测座300、设于检测座300上方的移液器400以及建压系统，检测座300用于接收试剂盒20装入，试剂盒20设有通过微孔连通的前池120和后池并装设有试剂、样本、安装头，其中建压系统包括压力室210、气压连接装置510、第一注射器222、第二注射器223、驱动件224、第一电磁阀SV1、第二电磁阀SV2、第三电磁阀SV3。
气压连接装置510与压力室210连接，用于向前池120或后池施加正压或负压实现前池120中的液体流向后池；第一注射器222与压力室210/移液器400连通，用于对压力室210建立正压或负压，还用于辅助移液器400进行移液，或者对前池120中的液体进行打气泡混匀；第二注射器223与移液器400连通用于进行移液；驱动件用于同时驱动第一注射器222和第二注射器223；第一电磁阀SV1将第一注射器222与压力室210、第二注射器223选择性连通；第二电磁阀SV2将第一电磁阀与压力 室210、外界大气选择性连通；第三电磁阀SV3将气压连接装置与压力室210选择性连通。
以上仅为本申请的实施方式，并非因此限制本申请的专利范围，凡是利用本申请说明书及附图内容所作的等效结构或等效流程变换，或直接或间接运用在其它相关的技术领域，均同理包括在本申请的专利保护范围内。

Claims (20)

  1. 一种POCT血细胞分析仪的使用方法，其特征在于，包括：
    接收开机触发信号；
    进行硬件自检；
    进行整机初始化；
    接收试剂盒装入；
    对试剂盒上的样本进行自动化样本前处理并在自动化样本前处理完成后进行样本检测。
  2. 根据权利要求1所述的使用方法，其特征在于：所述进行硬件自检后包括：
    判断移液器是否加载有安装头；
    若是，则移液器进行安装头卸载，若否则进行所述整机初始化；
    所述移液器进行安装头卸载之后包括：
    检测座出仓以供试剂盒取出；
    检测座回仓再进行所述整机初始化步骤。
  3. 根据权利要求1所述的使用方法，其特征在于，所述判断移液器是否加载有安装头的步骤中，还包括：
    判断检测座内是否安装有试剂盒。
  4. 根据权利要求2所述的使用方法，其特征在于，进行整机初始化的步骤之后，还包括：
    进行压力检测，判断气路管路中的压力是否达到阈值范围；
    进行信号检测，包括判断电磁屏蔽组件配合后的检测信号进而判断电磁屏蔽效果和/或判断电流、电压信号是否在阈值范围内；
    进入待机状态。
  5. 根据权利要求4所述的使用方法，其特征在于，所述进入待机状态的步骤之后包括：
    接收关机触发信号；
    判断试剂盒有无；
    若判断试剂盒为有时进一步判断仓门状态；
    若判断仓门为打开状态，则提示取出试剂盒并回到所述判断试剂盒有无的步骤；
    若判断仓门为关闭状态，则打开仓门并提示取出试剂盒再回到所述判断试剂盒有无的步骤；
    若判断试剂盒为无时进一步判断仓门状态；
    若判断仓门为打开状态，则关闭仓门并提示关闭电源；
    若判断仓门为关闭状态，则提示关闭电源。
  6. 根据权利要求4所述的使用方法，其特征在于，所述进入待机状态的步骤之后包括：
    接收样本ID设置和测量模式设置；
    接收启动测试触发信号；
    核对待测信息与测量模式是否匹配，若不匹配则重新接收样本ID设置和测量模式设置；
    若匹配则打开仓门且检测座出仓以接收试剂盒装入。
  7. 根据权利要求6所述的使用方法，其特征在于，还包括：
    进行试剂盒有无检测；
    若无则提示放入试剂盒；
    若有则自动关闭仓门并进行自动化配液测试。
  8. 根据权利要求7所述的使用方法，其特征在于，还包括：
    显示测试结果；
    提示测试结束。
  9. 根据权利要求8所述的使用方法，其特征在于，还包括：
    自动打开仓门并提示取出试剂盒。
  10. 根据权利要求9所述的使用方法，其特征在于，还包括：
    获取试剂盒已取出信号；
    自动关闭仓门。
  11. 一种POCT血细胞分析仪，其特征在于，包括：
    壳体；
    检测座，可伸入或伸出所述壳体，用于接收试剂盒装入，并往复运动于试剂盒加载工位和样本检测工位之间；
    移液器，设于所述壳体内并位于所述检测座上方，用于对所述试剂盒进行自动化样本前处理。
  12. 根据权利要求11所述的POCT血细胞分析仪，其特征在于，所述试剂盒包括通过微孔连通的前池和后池，所述POCT血细胞分析仪还包括建压系统，所述建压系统设于所述壳体内并用于使得待测液从所述前池流向所述后池以进行阻抗检测法检测。
  13. 根据权利要求12所述的POCT血细胞分析仪，其特征在于，所述POCT血细胞分析仪还包括用于与所述检测座相盖合的金属屏蔽盖，所述试剂盒包括与所述后池连通的压力作用腔，所述金属屏蔽盖设有与所述建压系统连接的气压连接装置，所述金属屏蔽盖与所述检测座相盖合时所述气压连接装置与所述压力作用腔密封对接。
  14. 根据权利要求12所述的POCT血细胞分析仪，其特征在于，所述移液器上设有检测件，所述移液器包括导气管和套设在所述导气管的外周的外套管，所述导气管用于加载装设于所述试剂盒上的安装头，在所述导气管加载所述安装头时，所述导气管插入所述安装头，以使得所述安装头推动所述外套管沿所述导气管的轴向产生位置变化，所述检测件用于检测所述外套管的位置进而判断所述导气管上是否套接有所述安装头。
  15. 根据权利要求12所述的POCT血细胞分析仪，其特征在于，所述移液器还包括退头机构，所述退头机构用于在所述导气管上升时止挡所述外套管上升以使得所述外套管将所述安装头卸载。
  16. 根据权利要求15所述的POCT血细胞分析仪，其特征在于，所述退头机构为相对所述导气管的轴向或者径向设置的电磁铁、电机、或者U型口挡板。
  17. 根据权利要求12所述的POCT血细胞分析仪，其特征在于，所述建压系统包括压力室、联动设置的第一注射器和第二注射器，所述第一注射器用于为所述压力室建立正压或负压，所述第二注射器与所述移液器连通，用于在所述移液器套接有安装头时利用所述安装头的内腔作为液体中转位吸取试剂和/或样本。
  18. 根据权利要求17所述的POCT血细胞分析仪，其特征在于，所述POCT血细胞分析仪包括第一导管和第二导管，所述第一导管连接于所述第二注射器和所述移液器之间，所述第二导管用于连接所述注射器和所述压力室，所述第一导管的内径小于所述第二导管的内径。
  19. 根据权利要求18所述的POCT血细胞分析仪，其特征在于，所述第一导管的外周设有防折件，所述防折件为套管、弹簧或者绑带。
  20. 一种POCT血细胞分析仪，其特征在于，包括检测座、设于所述检测座上方的移液器以及建压系统，所述检测座用于接收试剂盒装入，所述试剂盒设有通过微孔连通的前池和后池并装设有试剂、样本、安装头，其中所述建压系统包括：
    压力室；
    气压连接装置，与所述压力室连接，用于向所述前池或所述后池施加正压或负压实现所述前池中的液体流向所述后池；
    第一注射器，与所述压力室/移液器连通，用于对所述压力室建立正压或负压，还用于辅助所述移液器进行移液，或者对所述前池中的液体进行打气泡混匀；
    第二注射器，与所述移液器连通用于进行移液；
    驱动件，用于同时驱动所述第一注射器和所述第二注射器；
    第一电磁阀，将所述第一注射器与所述压力室、所述第二注射器选择性连通；
    第二电磁阀，将所述第一电磁阀与所述压力室、外界大气选择性连通；
    第三电磁阀，将所述气压连接装置与所述压力室选择性连通。
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