WO2022210888A1 - Agent bactéricide - Google Patents

Agent bactéricide Download PDF

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Publication number
WO2022210888A1
WO2022210888A1 PCT/JP2022/016027 JP2022016027W WO2022210888A1 WO 2022210888 A1 WO2022210888 A1 WO 2022210888A1 JP 2022016027 W JP2022016027 W JP 2022016027W WO 2022210888 A1 WO2022210888 A1 WO 2022210888A1
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Prior art keywords
water
disinfectant
present
alkaline ionized
solution
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PCT/JP2022/016027
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English (en)
Japanese (ja)
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和昭 浦田
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株式会社Nexting
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Priority claimed from JP2022054862A external-priority patent/JP7178069B2/ja
Publication of WO2022210888A1 publication Critical patent/WO2022210888A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the present invention relates to disinfectants.
  • Naturally derived disinfectants include, for example, those using grapefruit extract, tea catechin, and the like.
  • Nanosoy colloid is a water-soluble colloidal solution containing nanoized plant fatty acids, which is produced by mixing at least plant fatty acids (unsaturated fatty acids) such as soybean fatty acids, lecithin, and purified water and stirring them at high speed (patent References 1, 2, and Non-Patent Document 1).
  • Nanosoy colloids perform Brownian motion, destroying the ionic bonds of dirt components such as oil adhering to the object, making it easier for microorganisms to eat, making it possible to clean the object.
  • nanosoy colloid surrounds bacteria and viruses and pulls out their constituent molecules, so it can eliminate bacteria by changing bacteria into cells and viruses into proteins.
  • nanosoy colloids are mainly used by diluting the undiluted solution with water or hot water 5 to 1000 times depending on the application.
  • Most detergents other than nanosoy and colloid used after dilution are diluted with water or hot water.
  • the effect may vary depending on the dilution concentration and the type of the diluted solution. Therefore, by using a water-soluble colloidal solution containing micronized (preferably nanoized) soybean fatty acid (unsaturated fatty acid), such as nanosoy colloid, using a diluent other than water or hot water, the undiluted solution is diluted with water or hot water. It is conceivable to improve the effect when diluted, or to produce a different effect.
  • the present invention provides a sterilizing agent with improved sterilizing effect by diluting a water-soluble colloidal solution containing finely divided soybean fatty acid (unsaturated fatty acid) with weakly alkaline ionized water. intended to
  • One or more embodiments of the present invention have a pH of 8 per volume part of an aqueous colloidal solution containing micronized soybean fatty acids produced by mixing and stirring at least soybean fatty acids, lecithin, and purified water. It is a sterilizing agent obtained by mixing 1.5 to 19 parts by volume of weakly alkaline ionized water of 8 to 9.9.
  • One or more embodiments of the present invention is a disinfectant with a pH of 10.0-10.5.
  • the weakly alkaline ionized water is weakly alkaline hot spring water.
  • One or more embodiments of the present invention are disinfectants, wherein the weakly alkaline ionized water has a hardness of 2 or less.
  • One or more embodiments of the present invention is a disinfectant, wherein the water-soluble colloidal solution is Nanosoy Colloid (registered trademark) and/or the weakly alkaline ionized water is Hot Spring Water 99 (registered trademark). is.
  • the target of sterilization is at least one selected from the group consisting of Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, Legionella, feline infectious peritonitis virus, and influenza virus. It is a disinfectant that is.
  • a water-soluble colloidal solution containing finely divided soybean fatty acids (unsaturated fatty acids) is diluted with weakly alkaline ionized water to provide a sterilizing agent with improved sterilizing effect.
  • a sterilizing agent refers to an agent that can reduce a certain number of proliferative bacteria and viruses from an object. It means that it has a reducing effect. It should be noted that the present invention is not limited to the contents described below, and can be variously modified without departing from the scope of the invention.
  • the disinfectant of the present invention is produced by mixing a water-soluble colloidal solution (hereinafter referred to as undiluted solution) containing microparticles of soybean fatty acid (unsaturated fatty acid) and weakly alkaline ionized water. Specifically, 1.5 to 19 parts by volume of weakly alkaline ionized water with a pH of 8.8 to 9.9 is mixed with 1 part by volume of the stock solution.
  • the sterilizing agent of the present invention is produced by diluting the stock solution 2.5 to 20 times by volume using weakly alkaline ionized water as the diluent.
  • the amount of weakly alkaline ionized water having a pH of 8.8 to 9.9 is preferably 4 to 14 parts by volume, particularly preferably 4 to 9 parts by volume, per 1 part by volume of the stock solution.
  • the resulting disinfectant of the present invention has a pH of 10.0 to 10.5, preferably 10.1 to 10.3, particularly preferably 10.2 to 10.3.
  • weakly alkaline ionized water may be poured into the stock solution, or the stock solution may be poured into weakly alkaline ionized water.
  • the undiluted solution and weakly alkaline ionized water are mixed just by pouring, it is not necessary to stir after pouring.
  • Dilution conditions are also not particularly limited, but it is preferable to carry out at normal temperature and atmospheric pressure.
  • the stock solution is an aqueous solution containing at least soybean fatty acid, lecithin, and purified water, which is stirred to cut the molecules and made into fine particles (nanoparticles) of a size capable of undergoing Brownian motion, containing soybean fatty acid. be.
  • the size of the fine particles is not particularly limited as long as Brownian motion is possible as described above, but is preferably nano-sized.
  • the particle size of the fine particles is, for example, 0.1-1000 nm, 1-900 nm or 5-800 nm.
  • the particle size of fine particles is determined by known methods such as sieving method, sedimentation method, microscopic method, light scattering method, laser diffraction/scattering method, electrical resistance test, observation by transmission electron microscope and observation by scanning electron microscope. can be measured.
  • the particle size may be measured with a known particle size distribution meter.
  • the particle size may be an average particle size, a volume average particle size, an area average particle size, or the like for a plurality of particles to be measured.
  • the particle size may be an average particle size calculated from a number distribution or the like based on a measurement such as a laser diffraction/scattering method.
  • Patent Document 1 For example, in the method of Patent Document 1, first, 14 to 22 parts by weight of soybean fatty acid, 2 to 4 parts by weight of lecithin, 6 to 14 parts by weight of alkanolamide, 25 parts by weight of isooctylphenoxypolyethoxyethanol, and tetraethylenediamine as an auxiliary additive. A mixture containing 3 parts by weight of acetic acid and 42 parts by weight of distilled water is stirred at high speed. Next, a saponification method is applied to the stirred mixture to obtain a solution containing fine molecules of a size capable of undergoing Brownian motion. Then, the solution is adjusted for water content and pH to obtain a stock solution.
  • weakly alkaline ionized water which is a diluent
  • the weakly alkaline ionized water used for the diluent is weakly alkaline water having a pH of 8.0 to 11.0, preferably pH 8.8 to 9.9, particularly preferably pH 9.5 to 9.9.
  • the weakly alkaline ionized water used for the diluent may be one artificially produced by electrolysis of tap water or the like, but is preferably natural weakly alkaline ionized water, more preferably natural weakly alkaline ionized water.
  • Alkaline hot spring water for example, Hot Spring Water 99 (Registered Trademark SOC Co., Ltd. https://www.onsensui.com/company/).
  • the natural weakly alkaline ionized water is natural water with a high alkaline ion concentration that springs out from various parts of the world. It is water that is said not to exist. Hot spring water 99 shows almost no change in pH even after four years of storage in a sealed PET bottle.
  • the weakly alkaline ionized water used as the diluent is preferably soft water, particularly soft water with a hardness of 2 or less. This is because it is believed that the smaller the content of metal ions such as calcium and magnesium, the more stable the finely divided soybean fatty acid in water.
  • the sterilizing agent of the present invention the sterilizing agent obtained by multiplying the undiluted nanosoy colloid with the diluted hot spring water 99 by 2.5, 5, 10, 15, and 20 volumes (hereinafter referred to as the sterilizing agent of the present invention, respectively) Agents 2.5, 5, 10, 15, 20) have a disinfecting effect.
  • the sterilizing agents hereinafter referred to as comparative sterilizing agents 5 and 10, respectively
  • the sterilizing agents obtained by doubling the volume of the undiluted nanosoy colloid by 5 and 10 volumes with the diluted purified water also have a sterilizing effect.
  • the disinfectant 5 of the present invention is more effective than the comparative disinfectant 5
  • the disinfectant 10 of the present application is more effective than the comparative disinfectant 10.
  • the disinfectants 2.5, 5, 10, 15, and 20 of the present invention are obtained by doubling the volume of the undiluted nanosoy colloid with the diluted purified water by 2.5, 5, 10, 15, and 20. It can be said that the sterilization effect is higher than that of the fungicide.
  • Table 1 shows the pH of nanosoy colloid, hot spring water 99, and nanosoy colloid diluted 5, 10, 15, and 20 times by volume with hot spring water 99. From the pH when diluted 5, 10, 15 and 20 times by volume, the pH when diluted 2.5 times can be estimated to be 10.4. By the way, the pH of the nanosoy colloid and the hot spring water 99 has a wide range. Therefore, it can be said that the disinfecting agent of the present invention obtained by diluting the undiluted solution with dilute hot spring water 99 by 2.5 to 20 times its volume has a pH in the range of 10.0 to 10.5.
  • the sterilizing agent of the present invention can maintain its sterilizing effect even after long-term storage.
  • the long term means two months or more, and further half a year or more.
  • the sterilization effect decreases with the lapse of time. This is because the disinfectant of the present invention does not change in appearance or smell even after half a year or more of its production, whereas the comparative disinfectant 5 has a putrid smell after one month or more of its production. The water color begins to change. This is because the bacteria propagated in the comparative disinfectant 5, indicating that the disinfectant effect of the comparative disinfectant 5 is reduced. Thus, the disinfectant of the present invention can maintain the disinfectant effect for a longer period of time than the comparative disinfectant 5.
  • the disinfectant of the present invention has a disinfecting effect against Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, Legionella, black mold, feline infectious peritonitis virus (FIPV), and influenza virus.
  • Pseudomonas aeruginosa and Legionella spp. are reduced to a level where the number of bacteria is 1/100 or less and the number of bacteria is undetectable 1 minute after the addition of the disinfectant 5 of the present invention. has a strong bactericidal effect in a short time against Pseudomonas aeruginosa and Legionella spp.
  • the number of bacteria is 1/100 or less in 1 minute after adding the disinfectant 2.5, 5, 10, 15, and 20 of the present invention, and the number of bacteria is 1/100 or less in 5 minutes.
  • the disinfectants 2.5, 5, 10, 15 and 20 of the present invention have a strong disinfecting effect against Staphylococcus aureus in a short period of time.
  • the number of Escherichia coli is about 1/10 in 1 minute or 5 minutes after adding the disinfectant 5, 10, 15, 20 of the present invention, and the number of bacteria is less than 1/100 in 30 minutes or more, and the number of bacteria is almost undetectable.
  • the disinfectants 5, 10, 15, and 20 of the present invention have a disinfecting effect against E. coli, although they decrease to the level and require time.
  • the sterilizing agent 2.5 of the present invention reduces the number of bacteria to 1/100 or less to an undetectable level 1 minute after addition to Escherichia coli, and has a strong sterilizing effect in a short period of time.
  • the number of bacteria is reduced to 1/2 or less in 1 minute after adding the disinfectant 5 of the present invention, and the number of bacteria is reduced to 1/5 or less in 30 minutes or more, and the disinfectant 5 of the present invention is weak against black mold. It has an antibacterial effect. Five minutes after the addition of the disinfectant 5 of the present invention, the number of bacteria of the genus Salmonella is about 1/2, and after 30 minutes or more, the number of bacteria is reduced to about 1/3. It has a weak bactericidal effect against
  • the strength of the disinfecting effect of the disinfectant 5 of the present invention against the above bacteria is Pseudomonas aeruginosa, Legionella spp. > Staphylococcus aureus > Escherichia coli > Black mold > Salmonella spp.
  • the comparative sterilizing agent 5 (the undiluted solution diluted 5 times by volume with purified water) has a sterilizing effect against the above bacteria, the sterilizing effect is lower than that of the sterilizing agent of the present invention.
  • the number of Staphylococcus aureus is 1/100 or less, and the number of bacteria is reduced to a level that is almost undetectable after 30 minutes or more after addition. Since the number of bacteria is only reduced to about 1/10, it can be said that the sterilizing effect against the above bacteria is lower than that of the sterilizing agent 5 of the present invention. Therefore, the sterilizing agent 5 of the present invention, which uses weakly alkaline ionized water instead of purified water as the diluent, has an improved sterilizing effect against the above bacteria.
  • the feline infectious peritonitis virus when a sterilant that is diluted 20 to 80 times with purified water is added, the feline infectious peritonitis virus can be suppressed below the detection limit 10 minutes after addition. and had an antibacterial effect.
  • the upper limit of the dilution rate that can suppress the feline infectious peritonitis virus below the detection limit was 160 times dilution when the dilution liquid was hot spring water that is weakly alkaline ionized water.
  • comparative disinfectant 5 since it is 80-fold diluted, it can be said that comparative disinfectant 5 has a lower disinfecting effect against feline infectious peritonitis virus (FIPV) than the disinfectant of the present invention. Therefore, the sterilizing agent 5 of the present invention, which uses weakly alkaline ionized water instead of purified water as the diluent, has an improved sterilizing effect against feline infectious peritonitis virus (FIPV).
  • the disinfectant 5 of the present invention has an inactivation effect of 99.999% in 1 minute after addition, and the disinfectant 5 of the present invention has a disinfecting effect against the influenza virus. Furthermore, it can be said that the disinfectant 5 of the present invention has a bactericidal effect against influenza viruses.
  • the sterilizing agent 5 of the present invention which uses weakly alkaline ionized water instead of purified water as the diluent, has an improved sterilizing or sterilizing effect against the influenza virus.
  • the sterilizing agent of the present invention contains 1.5 parts weakly alkaline ionized water (diluted solution) per 1 part by volume of a water-soluble colloidal solution (undiluted solution) containing finely divided soybean fatty acid (unsaturated fatty acid). ⁇ 19 parts by volume blended to produce.
  • the disinfectant of the present invention has a disinfecting effect against Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, Legionella, black mold, feline infectious peritonitis virus (FIPV), and influenza virus.
  • the sterilizing effect of the sterilizing agent of the present invention is improved as compared with the case where purified water is used as the diluent.
  • the disinfectant of the present invention includes antiseptics (eg, phenoxyethanol, 1,2-hexanediol, natural plant extracts), antioxidants (eg, vitamin E tocopherol, natural plant extracts), fragrances (natural fragrances and synthetic fragrances). ) may be added.
  • antiseptics eg, phenoxyethanol, 1,2-hexanediol, natural plant extracts
  • antioxidants eg, vitamin E tocopherol, natural plant extracts
  • fragrances natural fragrances and synthetic fragrances
  • the effect of the disinfectant of the present invention will be explained using Examples 1 to 5 shown below.
  • nanosoy colloid (Wit NSC-Bas Co., Ltd.) was used as the stock solution.
  • the hot spring water 99 used as the diluent is from OSC Co., Ltd.
  • the sterilization effect is defined as a reduction in the number of bacteria to at least about 1/2 or less.
  • Table 2 shows the sterilization test results of the sterilizing agent 5 of the present invention produced by diluting the stock solution 5 times by volume with 99 of hot spring water.
  • Table 3 shows the results of the sterilization test of the comparative sterilizing agent 5 produced by diluting the stock solution 5 times by volume with purified water.
  • Table 4 shows the bactericidal test results of the comparative disinfectant 10 produced by diluting the stock solution 10 times by volume with purified water. In addition, this sterilization test was requested to Microbial Laboratory Co., Ltd. and performed.
  • Escherichia coli, Salmonella, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa were tested on a standard agar medium (Kyokuto Pharmaceutical Industry Co., Ltd.) at 35 ⁇ 1°C. , 24 hours, Legionella spp. on GVPC agar medium (Nissui Pharmaceutical Co., Ltd.) at 35 ⁇ 1°C for 3-7 days, Black mold on Sabouraud agar medium (Kyokuto Pharmaceutical Industry Co., Ltd.) at 25 ⁇ 1°C for 48 hours. Then, the cells were suspended in physiological saline.
  • each suspension was diluted with physiological saline so that the number of bacteria was about 5 ⁇ 10 6 to 2 ⁇ 10 7 CFU/mL. , and this was used as the bacterial solution.
  • Escherichia coli, Salmonella, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa were placed on a standard agar medium at 35 ⁇ 1°C for 24 hours, and Legionella was placed on a GVPC agar medium at 35 ⁇ 1°C for 3-7 days, followed by black mold. was cultured on a Sabouraud agar medium at 25 ⁇ 1° C. for 48 hours, and bacterial colonies were counted.
  • the sterilization test method using the comparative disinfectant 5 is as follows: Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa on standard agar medium (Kyokuto Pharmaceutical Industry Co., Ltd.) at 35 ⁇ 1 ° C for 24 hours, and Legionella on GVPC agar medium (Nissui Pharmaceutical Co., Ltd.) at 35 ⁇ 1° C. for 48 hours, and the cells were suspended in physiological saline. Each suspension was diluted with physiological saline so that the number of bacteria was approximately 1 to 2 ⁇ 10 7 CFU/mL while measuring the absorbance using UVmini-1240 (SHIMADZU), and this was used as the bacterial solution. did.
  • test product comparative disinfectant 5
  • the test solution and the control solution were diluted 100-fold with physiological saline, and 100 ⁇ L of each solution was smeared on the plate medium.
  • Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were cultured on a standard agar medium at 35 ⁇ 1° C.
  • Tables 2 and 4 show that the disinfectant 5 of the present invention and the comparative disinfectant 10 have a disinfecting effect against Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, and Legionella. Moreover, from Table 2, it can be seen that the sterilizing agent 5 of the present invention also has a sterilizing effect on black mold. Table 3 shows that the comparative disinfectant 5 has a disinfecting effect against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Legionella.
  • the disinfectant 5 of the present invention reduces the number of bacteria faster than the comparative disinfectant 5 against Escherichia coli and Staphylococcus aureus. From this, it can be seen that by changing the diluent from purified water to hot spring water 99 at the same dilution ratio, the sterilization effect against Escherichia coli and Staphylococcus aureus is improved. Further, from Tables 2 and 4, although the dilution ratio is different, the comparative disinfectant 10 has almost no disinfecting effect against black mold, whereas the disinfectant 5 of the present application has a disinfecting effect against black mold. From this, it can be seen that the sterilizing agent 5 of the present application has an improved sterilizing effect against black mold.
  • the disinfectant 5 of the present invention has a disinfecting effect against Escherichia coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, Legionella, and black mold.
  • hot spring water 99 is used as a diluted solution of the stock solution
  • the sterilization effect against Escherichia coli, Salmonella spp., Staphylococcus aureus, Pseudomonas aeruginosa, Legionella spp., and black mold is higher than when using purified water. I understand.
  • Table 5 shows the results of the E. coli sterilization test for the disinfectants 2, 5, 10, 15 and 2 of the present invention, which were produced by diluting the stock solution 2, 5, 10, 15 and 20 times by volume with hot spring water 99.
  • Table 6 shows the sterilization test results of Staphylococcus aureus in the disinfectants 2, 5, 10, 15, and 20 of the present invention produced by diluting the stock solution 2, 5, 10, 15, and 20 times by volume with hot spring water 99. show.
  • this sterilization test was requested to the Japan Institute for Microbiology Co., Ltd. and performed.
  • Escherichia coli and Staphylococcus aureus were cultured on a standard agar medium (Kyokuto Pharmaceutical Co., Ltd.) at 35 ⁇ 1° C. for 24 hours, and the cells were suspended in physiological saline. Each suspension was diluted with physiological saline so that the number of bacteria was about 1 to 2 ⁇ 10 7 CFU/mL while measuring the absorbance using an absorbance meter UVmini-1240 (SHIMADZU). liquid.
  • test and control solutions were diluted with saline to neutralize the bactericidal action.
  • Staphylococcus aureus diluted 2.5 times with hot spring water and 5 times with artificial alkaline ionized water 1 mL of the 1000-fold dilution was smeared on three plates.
  • 100 ⁇ l were plated for 100-fold dilutions.
  • E. coli and Staphylococcus aureus were cultured on a standard agar medium at 35 ⁇ 1°C for 24 hours, and bacterial colonies were counted.
  • 1 mL of the test product diluted 100 times with physiological saline and 10 ⁇ L of the bacterial solution were inoculated into 1 mL of physiological saline, and 1 minute later, 100 ⁇ L was applied to a plate. , a comparison of the number of colonies after culture was performed.
  • Staphylococcus aureus diluted 2.5 times with hot spring water and 5 times with artificial alkaline ionized water, which could not be confirmed to be invalidated, a similar experiment was performed with 1000 times dilution, and invalidation was confirmed.
  • the sterilizing agent of the present invention has a sterilizing effect on Escherichia coli and Staphylococcus aureus even when diluted 2.5, 10, 15, and 25 times by volume.
  • the strength of the disinfecting effect of the disinfectant 5 of the present invention is Pseudomonas aeruginosa, Legionella spp>Staphylococcus aureus>Escherichia coli>Black mold>Salmonella spp.
  • the sterilizing agents diluted by volume, 15 times by volume, and 25 times by volume have an effect of sterilizing at least Pseudomonas aeruginosa, Legionella spp.>Staphylococcus aureus>Escherichia coli.
  • Table 7 shows the results of the Staphylococcus aureus sterilization test of the disinfectant of the present invention produced by diluting the stock solution 5 times by volume with artificial alkaline ionized water. This sterilization test was conducted under the same conditions as the sterilization test for Staphylococcus aureus in Example 2 described above.
  • Artificial alkaline ionized water is water made by artificially electrolyzing water, and in this example, Kirin alkaline ionized water (pH 8.8-9.4, hardness 55-59) was used. board.
  • Tables 8 and 9 show the sterilization test results of Staphylococcus aureus and Legionella bacteria of hot spring water 99 and artificial alkaline ion water used as diluents.
  • the Staphylococcus aureus sterilization test was conducted under the same conditions as the Staphylococcus aureus sterilization test in Example 2 described above. Legionella bacteria are as follows.
  • Legionella was cultured on GVPC agar medium (Nissui Pharmaceutical Co., Ltd.) at 35 ⁇ 1° C. for 48 hours, and the cells were suspended in physiological saline. The suspension was diluted with physiological saline so that the number of bacteria was about 1 to 2 ⁇ 10 7 CFU/mL while measuring absorbance using UVmini-1240 (SHIMADZU), and this was used as a bacterial solution.
  • GVPC agar medium Nasui Pharmaceutical Co., Ltd.
  • UVmini-1240 SHIMADZU
  • the test solution and the control solution were diluted 100-fold with physiological saline, and 100 ⁇ L of each solution was smeared on the plate medium. Thereafter, the Legionella spp. was cultured on a standard agar medium at 35 ⁇ 1° C. for 72 hours, and the bacterial colonies were counted.
  • Table 11 shows the growth inhibitory effect test results of feline infectious peritonitis virus (FIPV) of water-diluted disinfectants obtained by diluting the stock solution with purified water at 20 volumes, 40 volumes, 80 volumes, 160 volumes, 320 volumes, and 640 volumes. indicates In addition, this growth inhibitory effect test was requested to Osaka Prefecture University graduate School of Life and Environmental Studies Department of Veterinary Medicine.
  • FIPV feline infectious peritonitis virus
  • the stock solution was diluted with purified water or hot spring water 99 to 20, 40, 80, 160, 320, and 640 times by volume, and each diluted solution was diluted 9: virus solution 1 ( volume) and allowed to react for 10 minutes, 30 minutes, and 1 hour at room temperature. As a control, it was reacted with a virus solution mixed with purified water or hot spring water 99 at the same ratio. Feline infectious peritonitis virus strain 79-1146 (field isolate) was used as the virus.
  • the virus solution was diluted with DMEM medium in 10 steps from 10 to 10 6 times.
  • the diluted virus was inoculated into the sheet-like fcwf cells and cultured for 2 days at 37° C. in the presence of 5% CO 2 . After that, the cytopathic effect of the virus was observed under an inverted microscope and evaluated.
  • the reaction and virus inoculation were performed twice, and the 50% tissue culture infectious dose TCID 50 /mL was determined for each by the Behrens-Karber method. Since the results were almost the same both times, Tables 5 and 6 show the results of the first time.
  • the cells were cultured by adding fetal bovine serum at a concentration of 10% to Dulbecco's Modified Eagle (DMEM) medium (Nissui Pharmaceutical Co., Ltd.).
  • DMEM Dulbecco's Modified Eagle
  • the hot spring water-diluted disinfectant can suppress the growth of the feline infectious peritonitis virus to below the detection limit up to 160 volume-fold dilutions. Since it is possible to suppress the proliferation of the feline infectious peritonitis virus to below the detection limit until the undiluted solution is diluted 160 times by volume with hot spring water, naturally the disinfectant of the present invention also suppresses the proliferation of the feline infectious peritonitis virus below the detection limit. It can be said that it can be suppressed. From Table 6, it can be seen that the water-diluted disinfectant can suppress the growth of feline infectious peritonitis virus to below the detection limit up to 80 volume-fold dilution.
  • the dilution ratio for suppressing the growth of feline infectious peritonitis virus to below the detection limit is 1/2 for the water-diluted disinfectant compared to the hot spring water-diluted disinfectant.
  • Table 6 it can be seen that even the undiluted solution can suppress the proliferation of feline infectious peritonitis virus to below the detection limit.
  • the disinfecting agent of the present invention has a disinfecting effect against feline infectious peritonitis virus, and when hot spring water 99 is used as the diluted solution of the stock solution, compared to using purified water, cat infection is more likely to occur. It can be seen that the sterilization effect against peritonitis virus is high.
  • Table 12 shows the results of the influenza virus inactivation effect test of the disinfectant 5 of the present invention obtained by diluting the stock solution 5 times by volume with hot spring water 99, and the comparative disinfectant 5 obtained by diluting the stock solution 5 times by volume with purified water. .
  • this inactivation effect test was requested to Food Environment Hygiene Laboratory Co., Ltd. and performed.
  • 0.1 mL of the virus solution was added to 0.9 mL each of the disinfectant 5 of the present invention and the comparative disinfectant 5 to prepare each test mixture.
  • 0.1 mL of virus solution was added to 0.9 mL of phosphate buffer to generate a control mixture.
  • the test and control mixtures were sensitized at room temperature (25° C.) after preparation for 0, 1 and 5 minutes after the start of the test.
  • the dilution ratio of the disinfectant 5 of the present invention and the comparative disinfectant 5 and the virus solution was determined in the preliminary cytotoxicity test, in the 10-fold solution of each of the disinfectant 5 of the present invention and the comparative disinfectant 5.
  • the decision was made by confirming the stunted growth of The swine influenza virus H 1 N 1 IOWA strain was used as the influenza virus, and the added concentration was 10 5 TCID 50 /mL.
  • the disinfectant 5 of the present invention has an inactivating effect on influenza viruses, and when hot spring water 99 is used as a dilution of the stock solution, it is more effective for influenza viruses than when using purified water. It can be seen that the inactivation effect of is high. Therefore, it can be seen that the sterilizing agent of the present invention has a high sterilizing effect on influenza viruses.
  • a water-soluble colloidal solution containing finely divided soybean fatty acids is used as the stock solution.
  • corn oil fatty acid canola oil fatty acid, soybean oil fatty acid, olive oil fatty acid, safflower oil fatty acid, pine oil fatty acid, grapeseed oil fatty acid, pine nut oil fatty acid, nut oil fatty acid. .

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Abstract

L'invention concerne un agent bactéricide qui est préparé par dilution d'une solution colloïdale hydrosoluble contenant un acide gras de soja microparticulaire (un acide gras insaturé) avec de l'eau ionisée faiblement alcaline et qui a un effet bactéricide amélioré.　L'agent bactéricide est produit en mélangeant 1 partie en volume d'une solution colloïdale hydrosoluble avec 1,5 à 19 parties en volume d'eau ionisée faiblement alcaline ayant une valeur de pH de 8,8 à 9,9, la solution colloïdale hydrosoluble contenant un acide gras de soja microparticulaire et étant produite par mélange et agitation d'au moins un acide gras de soja, de lécithine et d'eau purifiée ensemble. La bactérie à tuer comprend au moins une bactérie sélectionnée dans le groupe constitué par Escherichia coli, la bactérie Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa, la bactérie Legionella, le virus de la péritonite infectieuse féline et le virus de la grippe.
PCT/JP2022/016027 2021-03-30 2022-03-30 Agent bactéricide WO2022210888A1 (fr)

Applications Claiming Priority (4)

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JP2021057574 2021-03-30
JP2021-057574 2021-03-30
JP2022-054862 2022-03-30
JP2022054862A JP7178069B2 (ja) 2021-03-30 2022-03-30 除菌剤

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WO2022210888A1 true WO2022210888A1 (fr) 2022-10-06

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007197377A (ja) * 2006-01-27 2007-08-09 San Okinawa:Kk 月桃葉水性抽出液およびその用途
JP3153460U (ja) * 2008-12-23 2009-09-10 株式会社ウイット 環境、健康対応型多機能植物洗浄剤と洗浄剤を入れた注付蛇腹つきキャップ中・大型容器
JP2009292736A (ja) * 2008-06-02 2009-12-17 Fumakilla Ltd 抗ノロウイルス組成物
JP2012250959A (ja) * 2011-06-06 2012-12-20 Air Water Mach Inc 抗菌消臭剤
WO2015037743A1 (fr) * 2013-09-13 2015-03-19 株式会社ウイット Nouvelle composition d'agent nettoyant multifonction colloïdal utilisant acide gras végétal et lécithine, et procédé peu onéreux pour sa fabrication

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007197377A (ja) * 2006-01-27 2007-08-09 San Okinawa:Kk 月桃葉水性抽出液およびその用途
JP2009292736A (ja) * 2008-06-02 2009-12-17 Fumakilla Ltd 抗ノロウイルス組成物
JP3153460U (ja) * 2008-12-23 2009-09-10 株式会社ウイット 環境、健康対応型多機能植物洗浄剤と洗浄剤を入れた注付蛇腹つきキャップ中・大型容器
JP2012250959A (ja) * 2011-06-06 2012-12-20 Air Water Mach Inc 抗菌消臭剤
WO2015037743A1 (fr) * 2013-09-13 2015-03-19 株式会社ウイット Nouvelle composition d'agent nettoyant multifonction colloïdal utilisant acide gras végétal et lécithine, et procédé peu onéreux pour sa fabrication

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