WO2022197049A1 - Biocapteur utilisant une structure de puits à l'échelle millimétrique ou de puits à l'échelle microscopique - Google Patents

Biocapteur utilisant une structure de puits à l'échelle millimétrique ou de puits à l'échelle microscopique Download PDF

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Publication number
WO2022197049A1
WO2022197049A1 PCT/KR2022/003552 KR2022003552W WO2022197049A1 WO 2022197049 A1 WO2022197049 A1 WO 2022197049A1 KR 2022003552 W KR2022003552 W KR 2022003552W WO 2022197049 A1 WO2022197049 A1 WO 2022197049A1
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Prior art keywords
connection
milliwell
biosensor
module
electrode
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PCT/KR2022/003552
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English (en)
Korean (ko)
Inventor
배남호
이석재
이혜연
Original Assignee
주식회사 마라나노텍코리아
한국과학기술원
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Publication of WO2022197049A1 publication Critical patent/WO2022197049A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure

Definitions

  • the present invention relates to a biosensor using a milliwell or microwell structure, and more particularly, a connection module electrically connectable to an external device and a sensing module in which a milliwell or microwell structure is formed while being detachable from the connection module. It relates to a biosensor in which the sensing part can be replaced according to the present invention.
  • Target substances such as hormones, proteins, and pathogens, which enable prediction of human disease symptoms and progression, and further the condition of food, can be analyzed by immunoassay using antigen-antibody reactions.
  • analysis of target substances based on immunoassay has been usually performed in clinical laboratories equipped with special equipment.
  • the need for self-diagnosis at home and for examinations at medical sites in hospitals or emergency rooms has recently increased, the development of an immunoassay platform that does not require specialized knowledge or complicated processes and has a short analysis time has been continuously required. .
  • the electrochemical analysis method based on the biosensor analyzes the current generated by the redox reaction according to the contact between the electrode provided in the biosensor and the analyte sample, thereby qualitative and quantitative analysis of the target material in the analyte sample.
  • electrode material of the biosensor metals (eg, gold, platinum, palladium, etc.) having excellent electrical characteristics and not changing in the external environment may be used.
  • An electrode made of such a material may be manufactured in the form of a thin thin film electrode through sputtering or electroplating having cost competitiveness while maintaining the characteristics of the material.
  • a conventional biosensor is provided with an electrode including a sensing unit for sensing a target material and a connection unit connected to a measuring device.
  • the electrode 2 provided in the conventional biosensor 1 includes a sensing unit 2a for sensing a target material, and a substrate 3 extending from the sensing unit 2a to a predetermined length. It is disposed in a wider area than the sensing unit (2a) on the top, the end includes an extension (2b) that is electrically connected to the measuring device (4).
  • the biosensor 1 having the electrode 2 may be manufactured in a preset size through a dicing process.
  • the conventional biosensor 1 has the following problems. As the length of the electrode 2 provided in the conventional biosensor 1 is unnecessarily long, a small number of electrodes 2 have to be formed on a wafer having a limited area, resulting in low productivity. .
  • the conventional biosensor 1 is configured such that the electrode 2 is directly connected to the measuring device 4 . Therefore, in order to perform other types of tests, there is an inconvenience of having to replace all the biosensors 1 connected to the measuring equipment 4 .
  • connection part 4a of the measuring device 4 connected to the electrode 2 of the biosensor 1 is formed in the form of tongs fixed to the biosensor 1 by pressing the upper and lower surfaces of the biosensor 1 . Therefore, when the connection part 4a is connected to the biosensor 1, there is a possibility that the electrode 2 of the biosensor 1 may be damaged, and an unexpected external force is applied to the connection part 4a or the biosensor 1 When applied, there is a problem in that the connection part 4a of the measuring device 4 is easily separated from the biosensor 1 .
  • a dedicated measuring device 4 was used to measure the response of the biosensor 1 , but a household or individual is equipped with the above-described dedicated measuring device 4 to perform analysis of the biosensor 1 . It is not practically easy, and accordingly, it is impossible to use it in various environments or situations, so there is a problem that the utilization is very low.
  • connection module electrically connectable to an external device and a sensing module detachably attached to the connection module and formed with a milliwell or microwell structure. It relates to a possible biosensor.
  • a biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes: a connection module electrically connectable to an external device; A sensing module configured to detect a target material from the analyte sample introduced into the interior, and transmit an electrical signal generated by reacting with the sensed target material to the connection module; including, wherein the sensing module includes the analyte sample A sensor is provided to detect the target material from the It includes a plurality of electrodes for generating a ; and a milliwell or microwell structure provided on the electrode and comprising a plurality of grooves, wherein the sensing module is detachable from the connection module.
  • the electrode of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes a first electrode that oxidizes with the target material, and a second electrode that reacts with the target material by reduction; A third electrode configured to maintain a constant operating voltage between the first electrode and the second electrode may be included.
  • the milliwell or microwell structure of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems may be provided on the first electrode.
  • connection module of the biosensor using the milliwell or microwell structure of the present invention for solving the above-described problems includes a first connection terminal that can be coupled to the external device, a second connection terminal that can be coupled to the sensing module and , a controller configured to control transmission of a signal between the first connection terminal and the second connection terminal, and a main body in which the first connection terminal, the second connection terminal, and the controller are installed.
  • the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems is electrically connected to a connection pad electrically connected to the sensor and the connection pad through a bonding wire, It may further include a housing in which a connection port coupleable to the connection module, the sensor, the connection pad, and the connection port are installed.
  • the housing of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes a first member having a channel for guiding the analyte sample to the electrode of the sensor, and the first and a second member disposed opposite to the member and coupled to the first member on which the sensor, the connection pad, and the connection port are seated, and the milliwell or microwell structure may be provided in the channel.
  • connection module and the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems are detachably coupled to each other through magnetism, and can be electrically connected to each other through surface contact. have.
  • a plurality of the sensing modules of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems are provided and detachably coupled to the connection module, and the connection module is coupled to the external device
  • a controller comprising a first possible connection terminal and a second connection terminal capable of being coupled to the sensing module, the controller configured to control transmission of a signal between the first connection terminal and the second connection terminal, and the first connection terminal , and a main body in which the second connection terminal and the controller are installed, and a plurality of the second connection terminals may be provided.
  • connection module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems may include a power supply for controlling the connection between the second connection terminal and the controller.
  • the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems is provided with a plurality of sensing units including the sensors, and the plurality of sensing units are separated from each other through a partition wall. , may be electrically connected to each other through a connection port electrically coupleable to the connection module.
  • the present invention is configured to electrically connect a biosensor and an external device through a connection terminal provided in the form of a USB or pin, it can be applied to a variety of devices, thereby reducing costs, as well as being easily usable in real life for convenience and product It has the advantage of increasing the usability of
  • the present invention has an advantage in that the resolution can be improved by inserting an analyte sample through a milliwell or microwell structure, and the biosensor of the present invention includes a connection module electrically connected to an external device and detachable from the connection module Since it is composed of possible sensing modules, various types of inspections can be performed by replacing the sensing module, and only the sensing module can be replaced while the connection module is coupled to an external device, so it has the advantage of continuously and rapidly performing inspections. .
  • the present invention is configured so that the sensing module and the external device are electrically connected to each other through the connection module, the sensing part is prevented from being damaged by the external device even when the biosensor is coupled to or separated from the external device. have.
  • the present invention has an advantage in that the shape of the electrode provided on the substrate is improved, so that a larger number of biosensors can be manufactured compared to the prior art, thereby reducing the manufacturing cost.
  • 1 is a diagram schematically showing a conventional biosensor.
  • FIG. 2 is a state diagram showing a state in which a biosensor according to an embodiment of the present invention is connected to an external device:
  • 2A is a state diagram showing a state in which an external device in the form of a smartphone or tablet and a biosensor are connected
  • 2B is a state diagram showing a state in which an external device in the form of a PC and a biosensor are connected.
  • FIG. 3 is a perspective view illustrating a biosensor in which a milliwell or microwell structure is formed according to an embodiment of the present invention.
  • FIG. 4 is a plan view illustrating a disassembled state of a biosensor according to an embodiment of the present invention.
  • FIG. 5 is a cross-sectional view taken along line VI-VI of FIG. 4 .
  • FIG. 6 is a side view showing a portion “A” of FIG. 4 .
  • FIG. 7 is a plan view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
  • FIG. 8 is an enlarged view of part “B” of FIG. 7 .
  • FIG. 9 is a plan view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
  • FIG. 10 is a diagram schematically illustrating a process in which part “C” of FIG. 9 is coupled.
  • FIG. 11 is a side view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
  • a “module” or “unit” for a component performs at least one function or operation.
  • a “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software.
  • a plurality of “modules” or a plurality of “units” other than a “module” or “unit” that must be performed in specific hardware or are executed in at least one processor may be integrated into at least one module.
  • the singular expression includes the plural expression unless the context clearly dictates otherwise.
  • the present invention relates to a biosensor using a milliwell or microwell structure, and by using a connection module electrically connectable to an external device, and a sensing module detachably attached to the connection module and having a milliwell or microwell structure, the sensing part It relates to a biosensor that can be replaced.
  • a biosensor 10 using a milliwell or microwell structure uses a milliwell or microwell structure 220 to improve resolution, and the biosensor 10 uses a milliwell or microwell structure 220 . It is configured to be electrically connected to the external device 20 installed with software capable of measuring and analyzing the electrical signal generated in the .
  • the biosensor 10 is electrically connected to the external device 20 in the form of a smartphone or tablet as shown in FIG. can be electrically connected.
  • the external device 20 is not limited thereto, and may be used in various forms electrically connectable to the biosensor 10 .
  • the external device 20 has a terminal unit (not shown) that can be coupled to the first connection terminal 110 provided in the connection module 100 so as to be electrically connectable to the connection module 100 of the biosensor 10 to be described later. may be provided.
  • a program or a program capable of performing qualitative and quantitative analysis on a target material by measuring and analyzing the potential according to the redox reaction of the biosensor 10; Applications and the like may be installed. Through this, the external device 20 collects and analyzes signals generated by the biosensor 10, and can detect various diseases or diseases, or measure and display changes thereof.
  • the external device 20 may be connected to the Internet through wired or wireless communication and transmit data measured through the biosensor 10 to a user terminal or a server of a medical institution. Through the transmitted data, the subject or medical institution can monitor the health status in real time.
  • the external device 20 includes an input unit (not shown) electrically connected to the biosensor 10 to which the biosensor 10 is coupled, a signal (data) transmitted through the input unit, A control unit (not shown) for analyzing and diagnosing, a display unit (not shown) for outputting data transmitted from the control unit in an externally identifiable form, and a communication unit (not shown) for transmitting data received from the control unit to a selected user terminal and a medical institution server ) and a power supply unit (not shown) for supplying power to an input unit, a control unit, a display unit, and a communication unit.
  • the biosensor using a milliwell or microwell structure includes a connection module 100 , a sensing module 200 , and a sensor 210 .
  • connection module 100 a connection module 100
  • sensing module 200 a sensor 210
  • connection module 100 is electrically connectable to the external device 20 .
  • the connection module 100 includes a first connection terminal 110 , a second connection terminal 120 , a controller 130 , and a body 140 .
  • the first connection terminal 110 is coupled to the external device 20 to be electrically connectable to the external device 20 .
  • the first connection terminal 110 may have various configurations as long as it can be electrically connected to the external device 20 .
  • the first connection terminal 110 is coupled to the terminal provided in the external device 20 in a shape corresponding to the terminal of the external device 20 so as to be electrically connected to the external device 20 .
  • the first connection terminal 110 may be provided in the form of a USB, micro USB, or pin (PIN), but is not limited thereto, and various configurations are possible if it can be electrically connected to the external device 20 .
  • PIN pin
  • the second connection terminal 120 is coupled to the sensing module 200 to be described later and is electrically connectable to the sensing module 200 .
  • the second connection terminal 120 may be provided in the form of a micro USB or pin, but is not limited thereto, and various configurations may be used as long as it can be electrically connected to the sensing module 200 .
  • the controller 130 is electrically connected to the first connection terminal 110 and the second connection terminal 120, and a signal ( data) may be configured to control the transmission.
  • the controller 130 may be electrically connected to the first connection terminal 110 and the second connection terminal 120 through an electric wire, but is not limited thereto. If the two connection terminals 120 can be electrically connected, various configurations may be used.
  • the main body 140 includes a lower body in which the first connection terminal 110 , the second connection terminal 120 , and the controller 130 are installed, and is coupled to the lower body to provide the first connection terminal 110 .
  • the second connection terminal 120 and an upper body protecting an upper portion of the controller 130 may be included.
  • the sensing module 200 detects a target material from an analysis sample introduced therein, and transmits an electrical signal generated by reacting with the sensed target material to the connection module 100 .
  • a milliwell or microwell structure 220 is provided as configured to transmit.
  • the analysis sample may refer to a solution material, and may refer to a solution diluted with blood, serum, or plasma.
  • the analysis sample is not limited thereto, and as long as it is a solution material, it may be a variety of materials.
  • the sensing module 200 may include a sensor 210 that detects the target material from the analyte sample, reacts with the target material to generate the electrical signal, and the sensor 210 includes a substrate 211 , a plurality of electrodes 212 , and a milliwell or microwell structure 220 .
  • the senor 210 may be provided with a sensing material (eg, an enzyme, etc.) that reacts with the target material included in the analysis sample to detect the target material.
  • a sensing material eg, an enzyme, etc.
  • the sensor 210 When the sensor 210 comes into contact with the analysis sample, it interacts with a target material included in the analysis sample to generate an electrical signal.
  • the external device 20 connected to the biosensor 10 may analyze the electrical signal generated from the biosensor 10 to detect the presence or concentration of the target material.
  • the sensor 210 is not limited thereto, and may be configured to move, stop, filter, purify, react, and mix the analysis sample.
  • the substrate 211 provided in the sensor 210 may have a predetermined size, and the substrate 211 may have a size of 2 x 2 mm.
  • the size of the substrate 211 is not necessarily limited thereto, and may be changed to various sizes.
  • the substrate 211 may be made of a material that has elasticity and is bendable.
  • the substrate 211 may be a polyurethane (Poly urethane, PU)-based, polydimethylsiloxane (PDMS)-based, NOA (Noland Optical Adhesive)-based, epoxy-based, polyethylene terephthalate, At least one of PET), polymethyl methacrylate (PMMA), polyimide (PI), polystyrene (PS), polyethylene naphthalate (PEN), and polycarbonate (PC) It may be made of a material of PET), polymethyl methacrylate (PMMA), polyimide (PI), polystyrene (PS), polyethylene naphthalate (PEN), and polycarbonate (PC) It may be made of a material of
  • the material of the substrate 211 is not necessarily limited thereto, and electrodes generating a potential difference according to a reaction with a target material may be disposed, and various materials having flexibility may be applied.
  • the electrode 212 may be provided in plurality, and the electrode 212 may be provided on one surface of the substrate 211 and may react with a target material included in the analysis sample to generate an electrochemical signal. .
  • the plurality of electrodes 212 includes a first electrode 212a for oxidation or reduction reaction with a target material, a second electrode 212b for oxidation or reduction reaction with a target material in opposition to the reaction of the first electrode 212a, and a third electrode 212c configured to maintain a constant operating voltage between the first electrode 212a and the second electrode 212b.
  • the plurality of electrodes 212 may be formed on the insulating substrate 211 through a screen printing method.
  • the second electrode 212b and the third electrode 212c may be disposed to surround the first electrode 212a.
  • the first electrode 212a and the second electrode 212b may be disposed on the substrate 211 to be spaced apart from each other by a predetermined distance.
  • the first electrode 212a may have a circular shape
  • the second electrode 212b may have a semicircular shape to surround a portion of the first electrode 212a.
  • the arrangement shape of the first electrode 212a and the second electrode 212b and the shape of each of these electrodes are not limited thereto, and may be changed as necessary.
  • the first electrode 212a may refer to a working electrode that performs oxidation or reduction reaction by reaction with a target material or an analyte sample including the target material on the substrate 211 .
  • a bioreceptor specifically binding to a target material may be disposed on the first electrode 212a.
  • a pillar having a conductive layer deposited thereon is disposed on the first electrode 212a, and a bioreceptor such as an antibody, antigen, or aptamer may be disposed on the pillar.
  • a material that reacts with a target material to induce an electrochemical signal may be further disposed on the first electrode 212a.
  • the reaction area with the target material is widened, and through this, the biosensor 10 absorbs a small amount of the target material. It can also provide sensitive qualitative and quantitative analysis results.
  • the pillar may be a polymer structure having a nano size.
  • the pillar may be made of at least one of polyurethane, polydimethylsiloxane, Norland Optical Adhesives (NOA), epoxy, polyethylene terephthalate, polymethyl methacrylate, polyimide, polystyrene, polyethylene naphtharate, polycarbonate, and combinations thereof. have.
  • NOA Norland Optical Adhesives
  • the pillars may also be made of a combination of polyurethane and NOA (eg NOA 68).
  • NOA eg NOA 68
  • the pillars are not limited thereto, and may be made of more various polymers as long as they have flexibility.
  • the conductive layer deposited on the pillar may refer to a layer made of a conductive material.
  • the conductive layer may be formed of at least one of Ni, Zn, Pd, Ag, Cd, Pt, Ga, In, and Au, but is not limited thereto.
  • the second electrode 212b may refer to a counter electrode facing the first electrode 212a on the substrate 211 . Accordingly, if an oxidation reaction occurs in the first electrode 212a by reaction with a target material, a reduction reaction may occur in the second electrode 212b.
  • the above-described pillars having a conductive layer deposited thereon may be disposed on the second electrode 212b.
  • the third electrode 212c may refer to a reference electrode in which a potential is stably maintained even when in contact with a target material.
  • a reaction layer capable of maintaining a constant potential even when in contact with a target material may be provided on the third electrode 212c.
  • the reaction layer is Ag/AgCl, Ag, Hg2SO4, Ag/Ag+, Hg/Hg2SO4, RE-6H, Hg/HgO, Hg/Hg2Cl2, Ag/Ag2SO4, Cu/CuSO4, KCl saturated calomel half cell (SCE) and salt bridge
  • SCE KCl saturated calomel half cell
  • the potential difference of platinum may be made of a known material in advance.
  • the milliwell or microwell structure 220 is provided on the electrode 212 and includes a plurality of grooves.
  • the milliwell or microwell structure 220 may be provided at an inlet into which the analysis sample is inserted into the sensing module 200 , and the resolution is improved by using the milliwell or microwell structure 220 . There are advantages to doing it.
  • the milliwell or microwell structure 220 may be provided above a working electrode, and the milliwell or microwell structure 220 includes the first electrode 212a formed of a working electrode. It may be provided on the top.
  • the sensing module 200 may further include a connection pad 230 , a connection port 240 , a housing 250 , and a bonding wire 260 .
  • connection pads 230 may be provided, and may be electrically connected to the sensor 210 through the bonding wire 260 . 4 and 6,
  • a connection pad 213 for electrically connecting the plurality of connection pads 230 and the plurality of electrodes 212 provided in the sensor 210 to each other may be further provided on the bottom surface of the sensor 210 .
  • connection pad 213 attached to the bottom surface of the sensor 210 is electrically connected to the plurality of electrodes 212 provided in the sensor 210 , and the other surface of the connection pad 213 is the It may be electrically connected to the plurality of connection pads 230 through the bonding wire 260 .
  • the plurality of connection pads 230 and the connection pads 213 may be provided in a copper foil shape, and a surface of the plurality of connection pads 230 may be further coated with gold foil.
  • connection pad 213 and the sensor 210 may be electrically connected to each other through a conductive material (not shown), and a double-sided adhesive carbon tape may be used as the conductive material.
  • a conductive material is not limited thereto, and various materials may be used as long as the connection pad 213 and the sensor 210 can be electrically connected.
  • the bonding wire 260 is made of gold (Au), and one end of the bonding wire 260 is bonded to the connection pad 213 attached to the sensor 210 , and is provided in the sensor 210 .
  • the electrodes 212 may be electrically connected, and the other end of the bonding wire 260 may be bonded to the plurality of connection pads 230 to be electrically connected to the plurality of connection pads 230 .
  • connection port 240 may be electrically connected to the plurality of connection pads 230 , and may be coupled to the connection module 100 to be electrically connected to the connection module 100 .
  • connection port 240 may be electrically connected to the plurality of connection pads 230 through an electric wire 261 , and when coupled to the connection module 100 , the sensor ( Power may be supplied to the 210 , and an electrical signal generated from the sensor 210 may be transmitted to the connection module 100 .
  • the connection port 240 may be provided in a shape corresponding to the second connection terminal 120 provided in the connection module 100 .
  • the housing 250 includes the sensor 210 , the plurality of connection pads 230 , and the connection port 240 installed therein.
  • the housing 250 includes the sensor 210 and the plurality of connection pads ( 230), may be configured to surround the circumference of the connection port 240 to protect the sensor 210, the plurality of connection pads 230, and the connection port 240 from the outside.
  • the housing 250 is disposed opposite to the first member 251 and the first member 251 having a channel 253 for guiding the analyte sample provided from the outside to the electrode 212 of the sensor 210 . and is coupled to the first member 251 and includes a second member 252 on which the sensor 210 , the plurality of connection pads 230 , and the connection port 240 are seated.
  • the channel 253 is a hole formed through the first member 251 , and the channel 253 is an inclined surface 254 for guiding an analysis sample provided from the outside to the electrode 212 of the sensor 210 . ) is included.
  • the inclined surface 254 may be provided along the circumference of the channel 253 , and the analysis sample provided from the outside moves along the inclined surface 254 stably to the electrode 212 of the sensor 210 . be able to reach
  • the milliwell or microwell structure 220 may be provided in the channel 253 .
  • the milliwell or microwell structure 220 may be provided on the first electrode 212a-working electrode, and the channel 253 is the first electrode 212a-working electrode. (working electrode) may be provided on top.
  • the milliwell or microwell structure 220 is preferably provided in the channel 253 , and the milliwell or microwell structure 220 can be supported while being coupled to the channel 253 .
  • the milliwell or microwell structure 220 is provided in the channel 253 , it is possible to improve resolution when the analyzed sample is moved to the electrode 212 through the channel 253 .
  • the first member 251 may be coupled to the second member 252 to cover the sensor 210 and the connection port 240 disposed on the second member 252 to protect them from the outside. . Also, the first member 251 may press the sensor 210 and the connection port 240 with a predetermined force to firmly support them. In addition, the first member 251 prevents the analysis sample provided to the sensor 210 through the channel 253 from leaking to the outside of the housing 250 through coupling with the second member 252 . can do.
  • the sensor 210 , the plurality of connection pads 230 , and the connection port 240 are accommodated between the first member 251 and the second member 252 . and an accommodation space 250a for protecting the bonding wire 260 may be provided.
  • the accommodating space 250a may be provided in the form of an intaglio pattern inside the first member 251 and the second member 252, respectively.
  • At least one coupling protrusion 252a coupled to the first member 251 and supporting the first member 251 is provided on the second member 252 .
  • One member 251 may be provided with at least one coupling groove 251a corresponding to the coupling protrusion 252a.
  • the center of the channel 253 and the center of the sensor 210 may be naturally aligned at a predetermined position.
  • a sealer made of a rubber or silicone material that seals the space between the coupling protrusion 252a and the coupling groove 251a. may be further provided.
  • the second member 252 has a seating groove 252b in which the sensor 210 is seated and supported, and a collection groove 252c in which an analysis sample flowing down from the sensor 210 is stored. This may be further provided.
  • a sample guide surface 252d for guiding the analysis sample flowing out of the sensor 210 toward the collection groove 252c may be further provided between the sensor 210 and the collection groove 252c, and the sample guide surface (252d) may be formed in an inclined shape.
  • the housing 250 includes a flow state of an analyte sample, leakage of an analyte sample, an alignment state of the channel 253 and the sensor 210 , and the bonding wire 260 through
  • the sensor 210 and the plurality of connection pads 230 are connected to each other and the electrical connection between the plurality of connection pads 230 and the connection port 240 can be easily observed from the outside, and it is made of a transparent material. can be
  • the housing 250 is made of at least one of polymethyl methacrylate, polycarbonate, cyclic olefin copolymer, polyethylene sulfone, and polystyrene, or at least two or more of them. It may be provided with these combined materials.
  • the material of the housing 250 is not necessarily limited thereto, and may be made of a polydimethylsiloxane material, which is a silicone-based organic polymer.
  • the biosensor using the milliwell or microwell structure according to an embodiment of the present invention may be modified and used as follows.
  • a plurality of sensing modules 200 of the biosensor may be provided, and the plurality of sensing modules 200 are detachably coupled to the connection module 100 .
  • the connection module 100 may be electrically connected to the plurality of sensing modules 200 .
  • connection module 100 includes the first connection terminal 110 that can be coupled to the external device 20 and a second connection terminal 120 that can be coupled to the sensing module 200, A plurality of 2 connection terminals 120 may be provided.
  • the plurality of sensing modules 200 may be selectively coupled to the plurality of second connection terminals 120 .
  • the controller 130 is configured to operate through the plurality of second connection terminals 120 . After storing the order in which the electrical signals are transmitted, the electrical signals of the sensing module 200 connected to the corresponding second connection terminal 120 may be sequentially received and transmitted to the first connection terminal 110 .
  • the connection module 100 is a power supply unit 150 for controlling the connection between the plurality of second connection terminals 120 and the controller 130 between the plurality of second connection terminals 120 and the controller 130 . may further include.
  • a plurality of the power supply unit 150 may be provided, and may be individually connected to the plurality of second connection terminals 120 . Also, the power supply unit 150 may be connected to each of the controllers 130 .
  • the power supply unit 150 may be configured to be ON/OFF controlled through a user's operation. Only when the power supply unit 150 is controlled to an ON state through a user's operation, the power supply unit 150 is connected to the second connection terminal 120 . An electrical signal of the connected sensing module 200 may be transmitted to the controller 130 .
  • connection module 100 is installed on the periphery of the first connection terminal 110, the angle adjusting unit ( 160) may be further included.
  • the angle adjusting unit 160 is coupled to the first connection terminal 110 and is installed on the main body 140 and the rotating member 161 configured to rotate together with the first connection terminal 110 and is provided inside the
  • the rotating member 161 may be accommodated and may include a guide member 162 for guiding the rotation of the rotating member 161 .
  • a plurality of projections 161a spaced apart from each other are provided on the outer peripheral surface of the rotating member 161, and a plurality of grooves 162a corresponding to the plurality of projections 161a are provided on the inner peripheral surface of the guide member 162.
  • the user can arrange the plurality of second connection terminals 120 in predetermined positions by rotating the main body 140 in a state in which the first connection terminal 110 is coupled to the external device 20 . have.
  • connection module 100 and the sensing module 200 may be detachably coupled to each other through magnetism, and may be electrically connected to each other through surface contact. .
  • connection module 100 may include a first coupling member 170
  • the sensing module 200 may include a second coupling member 270 capable of being coupled to the first coupling member 170 .
  • the first coupling member 170 includes a coupling protrusion 171 protruding from one end of the connection module 100 and a first magnetic body 172 accommodated at an end of the coupling protrusion 171 .
  • the coupling protrusion 171 may be formed in a wedge structure in which the size of the outer diameter gradually decreases along the protrusion direction. Through this, when the connection module 100 and the sensing module 200 are coupled, an inclined outer circumferential surface of the coupling protrusion 171 is a coupling groove of the second coupling member 270 provided in the sensing module 200 . Guided to the inner peripheral surface of the (271) can be easily coupled.
  • the second coupling member 270 is formed concavely on the other end of the sensing module 200 facing one end of the connection module 100 , and the coupling groove 271 is formed inside the coupling groove 271 . It is accommodated in the first magnetic body 172 and may include a second magnetic body 272 capable of being coupled through a magnetic force.
  • connection module 100 and the sensing module 200 are primarily coupled through the structural coupling of the coupling protrusion 171 and the coupling groove 271, and the first magnetic body 172 and the second As the magnetic body 272 is secondarily coupled through coupling using the magnetism, they may be stably fixed to each other.
  • the sensing module 200 has the magnetic force of the second magnetic body 272 shielding the magnetic force of the second magnetic body 272 so as not to affect other parts of the sensing module 200.
  • a shielding structure 280 configured to do so may be provided.
  • the shielding structure 280 may be provided inside the housing 250 and be provided in a shape surrounding the coupling groove 271 of the second coupling member 270 and the periphery of the second magnetic body 272 . have.
  • the shielding structure 280 may be made of the same material as the housing 250 , and may be made of a material capable of shielding magnetic force.
  • connection module 100 and the sensing module 200 may be electrically connected to each other through surface contact coupling.
  • the second connection terminal 120 of the connection module 100 connected to the connection port 240 of the sensing module 200 is a flat plate type that can be electrically connected to each other through surface contact. It may be provided in the form of a terminal.
  • connection port 240 and the second connection terminal 120 can be electrically connected to each other just by being interviewed.
  • connection module 100 and the sensing module 200 This makes it easier to combine and separate the connection module 100 and the sensing module 200, and it is possible to prevent the connection port 240 and the second connection terminal 120 from being damaged even in a detachable type.
  • An identification film may be attached to the end of the sensing module 200 according to an embodiment of the present invention to confirm whether or not the sensing module 200 is used through the presence or absence of damage.
  • a receiving groove (not shown) capable of engaging with a wedge-shaped pressing protrusion (not shown) provided at the end of the connection module 100 is provided, and at the entrance of the receiving groove, the sensing module ( 200) may be attached to the identification film in the form of a thin film configured to block the entrance of the receiving groove.
  • the identification film may be configured to be damaged by being pressed with a predetermined force by the pressing protrusion of the connection module 100 inserted into the receiving groove when the sensing module 200 and the connection module 100 are coupled. Accordingly, the user can determine whether the sensing module 200 is used by checking whether the identification film provided at the end of the sensing module 200 is damaged.
  • the configuration capable of confirming whether the sensing module 200 is used is not necessarily limited thereto, and may be changed and applied in various forms.
  • the sensing module 200 may include a plurality of sensing units configured to detect a target material from an analysis sample.
  • the plurality of sensing units are electrically connected to the connection port 240 , and may be installed inside the housing 250 .
  • Each of the plurality of sensing units includes the sensor 210 , and the plurality of sensing units are separated from each other through a partition wall 214 , through the connection port 240 electrically coupleable to the connection module 100 . They can be electrically connected to each other.
  • the plurality of sensing units may include a first sensing unit 210a and a second sensing unit 210b.
  • the first sensing unit 210a and the second sensing unit 210b may be disposed in spaces independent from each other through the partition wall 214 provided in the housing 250 .
  • the first sensing unit 210a and the second sensing unit 210b each sense a target material from the contacted analyte, and the sensor 210 and the bonding wire that react with the target material to generate an electrical signal
  • the connection pad 230 may be electrically connected to the sensor 210 through 260 and electrically connected to the connection port 240 through the electric wire 261 .
  • first sensing unit 210a and the second sensing unit 210b may each include the channel 253 , and each of the channels 253 has the milliwell or microwell structure 220 . may be provided.
  • connection pad 230 and the connection port 240 are provided.
  • a power supply unit 290 for controlling the connection may be further provided.
  • the power supply unit 290 is electrically connected to the connection pad 230 and the connection port 240 , and may be configured to be ON/OFF controlled through a user's manipulation. Accordingly, only when the power supply unit 290 is controlled to be in the ON state through a user's manipulation, the electrical signal of the sensor 210 connected to the connection pad 230 may be transmitted to the connection port 240 .
  • the biosensor using the milliwell or microwell structure according to the embodiment of the present invention has been described as including the first sensing unit 210a and the second sensing unit 210b, but is not limited thereto, and 2 More than one sensing unit may be provided.
  • the biosensor using the milliwell or microwell structure according to the embodiment of the present invention described above has the following effects.
  • the biosensor 10 using a milliwell or microwell structure is configured to be electrically connected to the external device 20 through a connection terminal provided in the form of a USB or pin, so it can be applied to various devices. Accordingly, it is possible to reduce costs, as well as to be easily used in real life, so that convenience and usability of the product can be increased.
  • the biosensor 10 using a milliwell or microwell structure includes a connection module 100 electrically connected to an external device 20 , and sensing detachable from the connection module 100 . Since it is composed of the module 200, various types of tests can be performed by replacing the sensing module 200, and only the sensing module 200 can be replaced while the connection module 100 is coupled to the external device 20 Thus, it is possible to perform continuous and rapid inspection.
  • the biosensor 10 using a milliwell or microwell structure is configured such that the sensing module 200 and the external device 20 are electrically connected to each other via the connection module 100 . Therefore, even when the biosensor 10 is coupled to or separated from the external device 20 , the sensing portion is prevented from being damaged by the external device 20 .
  • the shape of the electrode provided on the substrate 211 applied to the biosensor 10 using the milliwell or microwell structure according to the embodiment of the present invention is improved, a larger number of sensors 210 than in the prior art It is possible to manufacture the , so that the manufacturing cost can be reduced.
  • the sensor 210 can be manufactured in a size of 2 x 2 mm. Therefore, the number of sensors 210 that can be manufactured using an existing 8-inch wafer has greatly increased from a low of 1000 to a high of 20,000. Except for the sensor 210, the manufacturing cost can be further reduced by using a low-cost PCB process.
  • the biosensor 10 using a milliwell or microwell structure has the advantage of improving resolution by inserting an analysis sample through the milliwell or microwell structure 220 . have.
  • connection module 110 ...first connection terminal

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  • Pathology (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La présente invention concerne un biocapteur utilisant une structure de puits à l'échelle millimétrique ou de puits à l'échelle microscopique, comprenant : un module de connexion qui peut être connecté électriquement à un dispositif externe ; et un module de détection configuré pour détecter un matériau cible à partir d'un échantillon d'analyse introduit dans celui-ci, et transmettre un signal électrique généré par réaction avec le matériau cible détecté, au module de connexion, le module de détection comprenant un capteur qui détecte le matériau cible à partir de l'échantillon d'analyse, et réagit avec le matériau cible pour générer le signal électrique ; le capteur comprend un substrat, une pluralité d'électrodes disposées sur une surface du substrat et réagissant avec le matériau cible pour générer un signal électrochimique, et une structure de puits à l'échelle millimétrique ou de puits à l'échelle microscopique disposée sur les électrodes et comprenant une pluralité de rainures ; et le module de détection peut être détaché du module de connexion.
PCT/KR2022/003552 2021-03-17 2022-03-14 Biocapteur utilisant une structure de puits à l'échelle millimétrique ou de puits à l'échelle microscopique WO2022197049A1 (fr)

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KR10-2021-0034702 2021-03-17
KR1020210034702A KR20220129854A (ko) 2021-03-17 2021-03-17 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6454345A (en) * 1987-08-26 1989-03-01 Matsushita Electric Ind Co Ltd Biosensor
KR20020088521A (ko) * 2001-05-18 2002-11-29 주식회사 아이센스 시료도입의 능력을 향상시킨 크로마토그래피 기능의다공성 박막이 구비된 바이오센서
JP2003532116A (ja) * 2000-04-25 2003-10-28 オンコプローブ・リミテッド 電気化学的センシング
JP2005164387A (ja) * 2003-12-02 2005-06-23 Osaka Industrial Promotion Organization Ｄｎａチップおよびそれを用いたバイオセンサー
JP2018515745A (ja) * 2015-05-12 2018-06-14 インディアン インスティテゥート オブ サイエンスＩｎｄｉａｎ Ｉｎｓｔｉｔｕｔｅ Ｏｆ Ｓｃｉｅｎｃｅ クレアチニンおよびアルブミン／クレアチニン比を検出するための装置および方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6454345A (en) * 1987-08-26 1989-03-01 Matsushita Electric Ind Co Ltd Biosensor
JP2003532116A (ja) * 2000-04-25 2003-10-28 オンコプローブ・リミテッド 電気化学的センシング
KR20020088521A (ko) * 2001-05-18 2002-11-29 주식회사 아이센스 시료도입의 능력을 향상시킨 크로마토그래피 기능의다공성 박막이 구비된 바이오센서
JP2005164387A (ja) * 2003-12-02 2005-06-23 Osaka Industrial Promotion Organization Ｄｎａチップおよびそれを用いたバイオセンサー
JP2018515745A (ja) * 2015-05-12 2018-06-14 インディアン インスティテゥート オブ サイエンスＩｎｄｉａｎ Ｉｎｓｔｉｔｕｔｅ Ｏｆ Ｓｃｉｅｎｃｅ クレアチニンおよびアルブミン／クレアチニン比を検出するための装置および方法

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