WO2022197049A1 - Biosensor using milliwell or microwell structure - Google Patents
Biosensor using milliwell or microwell structure Download PDFInfo
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- WO2022197049A1 WO2022197049A1 PCT/KR2022/003552 KR2022003552W WO2022197049A1 WO 2022197049 A1 WO2022197049 A1 WO 2022197049A1 KR 2022003552 W KR2022003552 W KR 2022003552W WO 2022197049 A1 WO2022197049 A1 WO 2022197049A1
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- milliwell
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0663—Whole sensors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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Definitions
- the present invention relates to a biosensor using a milliwell or microwell structure, and more particularly, a connection module electrically connectable to an external device and a sensing module in which a milliwell or microwell structure is formed while being detachable from the connection module. It relates to a biosensor in which the sensing part can be replaced according to the present invention.
- Target substances such as hormones, proteins, and pathogens, which enable prediction of human disease symptoms and progression, and further the condition of food, can be analyzed by immunoassay using antigen-antibody reactions.
- analysis of target substances based on immunoassay has been usually performed in clinical laboratories equipped with special equipment.
- the need for self-diagnosis at home and for examinations at medical sites in hospitals or emergency rooms has recently increased, the development of an immunoassay platform that does not require specialized knowledge or complicated processes and has a short analysis time has been continuously required. .
- the electrochemical analysis method based on the biosensor analyzes the current generated by the redox reaction according to the contact between the electrode provided in the biosensor and the analyte sample, thereby qualitative and quantitative analysis of the target material in the analyte sample.
- electrode material of the biosensor metals (eg, gold, platinum, palladium, etc.) having excellent electrical characteristics and not changing in the external environment may be used.
- An electrode made of such a material may be manufactured in the form of a thin thin film electrode through sputtering or electroplating having cost competitiveness while maintaining the characteristics of the material.
- a conventional biosensor is provided with an electrode including a sensing unit for sensing a target material and a connection unit connected to a measuring device.
- the electrode 2 provided in the conventional biosensor 1 includes a sensing unit 2a for sensing a target material, and a substrate 3 extending from the sensing unit 2a to a predetermined length. It is disposed in a wider area than the sensing unit (2a) on the top, the end includes an extension (2b) that is electrically connected to the measuring device (4).
- the biosensor 1 having the electrode 2 may be manufactured in a preset size through a dicing process.
- the conventional biosensor 1 has the following problems. As the length of the electrode 2 provided in the conventional biosensor 1 is unnecessarily long, a small number of electrodes 2 have to be formed on a wafer having a limited area, resulting in low productivity. .
- the conventional biosensor 1 is configured such that the electrode 2 is directly connected to the measuring device 4 . Therefore, in order to perform other types of tests, there is an inconvenience of having to replace all the biosensors 1 connected to the measuring equipment 4 .
- connection part 4a of the measuring device 4 connected to the electrode 2 of the biosensor 1 is formed in the form of tongs fixed to the biosensor 1 by pressing the upper and lower surfaces of the biosensor 1 . Therefore, when the connection part 4a is connected to the biosensor 1, there is a possibility that the electrode 2 of the biosensor 1 may be damaged, and an unexpected external force is applied to the connection part 4a or the biosensor 1 When applied, there is a problem in that the connection part 4a of the measuring device 4 is easily separated from the biosensor 1 .
- a dedicated measuring device 4 was used to measure the response of the biosensor 1 , but a household or individual is equipped with the above-described dedicated measuring device 4 to perform analysis of the biosensor 1 . It is not practically easy, and accordingly, it is impossible to use it in various environments or situations, so there is a problem that the utilization is very low.
- connection module electrically connectable to an external device and a sensing module detachably attached to the connection module and formed with a milliwell or microwell structure. It relates to a possible biosensor.
- a biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes: a connection module electrically connectable to an external device; A sensing module configured to detect a target material from the analyte sample introduced into the interior, and transmit an electrical signal generated by reacting with the sensed target material to the connection module; including, wherein the sensing module includes the analyte sample A sensor is provided to detect the target material from the It includes a plurality of electrodes for generating a ; and a milliwell or microwell structure provided on the electrode and comprising a plurality of grooves, wherein the sensing module is detachable from the connection module.
- the electrode of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes a first electrode that oxidizes with the target material, and a second electrode that reacts with the target material by reduction; A third electrode configured to maintain a constant operating voltage between the first electrode and the second electrode may be included.
- the milliwell or microwell structure of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems may be provided on the first electrode.
- connection module of the biosensor using the milliwell or microwell structure of the present invention for solving the above-described problems includes a first connection terminal that can be coupled to the external device, a second connection terminal that can be coupled to the sensing module and , a controller configured to control transmission of a signal between the first connection terminal and the second connection terminal, and a main body in which the first connection terminal, the second connection terminal, and the controller are installed.
- the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems is electrically connected to a connection pad electrically connected to the sensor and the connection pad through a bonding wire, It may further include a housing in which a connection port coupleable to the connection module, the sensor, the connection pad, and the connection port are installed.
- the housing of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes a first member having a channel for guiding the analyte sample to the electrode of the sensor, and the first and a second member disposed opposite to the member and coupled to the first member on which the sensor, the connection pad, and the connection port are seated, and the milliwell or microwell structure may be provided in the channel.
- connection module and the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems are detachably coupled to each other through magnetism, and can be electrically connected to each other through surface contact. have.
- a plurality of the sensing modules of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems are provided and detachably coupled to the connection module, and the connection module is coupled to the external device
- a controller comprising a first possible connection terminal and a second connection terminal capable of being coupled to the sensing module, the controller configured to control transmission of a signal between the first connection terminal and the second connection terminal, and the first connection terminal , and a main body in which the second connection terminal and the controller are installed, and a plurality of the second connection terminals may be provided.
- connection module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems may include a power supply for controlling the connection between the second connection terminal and the controller.
- the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems is provided with a plurality of sensing units including the sensors, and the plurality of sensing units are separated from each other through a partition wall. , may be electrically connected to each other through a connection port electrically coupleable to the connection module.
- the present invention is configured to electrically connect a biosensor and an external device through a connection terminal provided in the form of a USB or pin, it can be applied to a variety of devices, thereby reducing costs, as well as being easily usable in real life for convenience and product It has the advantage of increasing the usability of
- the present invention has an advantage in that the resolution can be improved by inserting an analyte sample through a milliwell or microwell structure, and the biosensor of the present invention includes a connection module electrically connected to an external device and detachable from the connection module Since it is composed of possible sensing modules, various types of inspections can be performed by replacing the sensing module, and only the sensing module can be replaced while the connection module is coupled to an external device, so it has the advantage of continuously and rapidly performing inspections. .
- the present invention is configured so that the sensing module and the external device are electrically connected to each other through the connection module, the sensing part is prevented from being damaged by the external device even when the biosensor is coupled to or separated from the external device. have.
- the present invention has an advantage in that the shape of the electrode provided on the substrate is improved, so that a larger number of biosensors can be manufactured compared to the prior art, thereby reducing the manufacturing cost.
- 1 is a diagram schematically showing a conventional biosensor.
- FIG. 2 is a state diagram showing a state in which a biosensor according to an embodiment of the present invention is connected to an external device:
- 2A is a state diagram showing a state in which an external device in the form of a smartphone or tablet and a biosensor are connected
- 2B is a state diagram showing a state in which an external device in the form of a PC and a biosensor are connected.
- FIG. 3 is a perspective view illustrating a biosensor in which a milliwell or microwell structure is formed according to an embodiment of the present invention.
- FIG. 4 is a plan view illustrating a disassembled state of a biosensor according to an embodiment of the present invention.
- FIG. 5 is a cross-sectional view taken along line VI-VI of FIG. 4 .
- FIG. 6 is a side view showing a portion “A” of FIG. 4 .
- FIG. 7 is a plan view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
- FIG. 8 is an enlarged view of part “B” of FIG. 7 .
- FIG. 9 is a plan view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
- FIG. 10 is a diagram schematically illustrating a process in which part “C” of FIG. 9 is coupled.
- FIG. 11 is a side view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
- a “module” or “unit” for a component performs at least one function or operation.
- a “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software.
- a plurality of “modules” or a plurality of “units” other than a “module” or “unit” that must be performed in specific hardware or are executed in at least one processor may be integrated into at least one module.
- the singular expression includes the plural expression unless the context clearly dictates otherwise.
- the present invention relates to a biosensor using a milliwell or microwell structure, and by using a connection module electrically connectable to an external device, and a sensing module detachably attached to the connection module and having a milliwell or microwell structure, the sensing part It relates to a biosensor that can be replaced.
- a biosensor 10 using a milliwell or microwell structure uses a milliwell or microwell structure 220 to improve resolution, and the biosensor 10 uses a milliwell or microwell structure 220 . It is configured to be electrically connected to the external device 20 installed with software capable of measuring and analyzing the electrical signal generated in the .
- the biosensor 10 is electrically connected to the external device 20 in the form of a smartphone or tablet as shown in FIG. can be electrically connected.
- the external device 20 is not limited thereto, and may be used in various forms electrically connectable to the biosensor 10 .
- the external device 20 has a terminal unit (not shown) that can be coupled to the first connection terminal 110 provided in the connection module 100 so as to be electrically connectable to the connection module 100 of the biosensor 10 to be described later. may be provided.
- a program or a program capable of performing qualitative and quantitative analysis on a target material by measuring and analyzing the potential according to the redox reaction of the biosensor 10; Applications and the like may be installed. Through this, the external device 20 collects and analyzes signals generated by the biosensor 10, and can detect various diseases or diseases, or measure and display changes thereof.
- the external device 20 may be connected to the Internet through wired or wireless communication and transmit data measured through the biosensor 10 to a user terminal or a server of a medical institution. Through the transmitted data, the subject or medical institution can monitor the health status in real time.
- the external device 20 includes an input unit (not shown) electrically connected to the biosensor 10 to which the biosensor 10 is coupled, a signal (data) transmitted through the input unit, A control unit (not shown) for analyzing and diagnosing, a display unit (not shown) for outputting data transmitted from the control unit in an externally identifiable form, and a communication unit (not shown) for transmitting data received from the control unit to a selected user terminal and a medical institution server ) and a power supply unit (not shown) for supplying power to an input unit, a control unit, a display unit, and a communication unit.
- the biosensor using a milliwell or microwell structure includes a connection module 100 , a sensing module 200 , and a sensor 210 .
- connection module 100 a connection module 100
- sensing module 200 a sensor 210
- connection module 100 is electrically connectable to the external device 20 .
- the connection module 100 includes a first connection terminal 110 , a second connection terminal 120 , a controller 130 , and a body 140 .
- the first connection terminal 110 is coupled to the external device 20 to be electrically connectable to the external device 20 .
- the first connection terminal 110 may have various configurations as long as it can be electrically connected to the external device 20 .
- the first connection terminal 110 is coupled to the terminal provided in the external device 20 in a shape corresponding to the terminal of the external device 20 so as to be electrically connected to the external device 20 .
- the first connection terminal 110 may be provided in the form of a USB, micro USB, or pin (PIN), but is not limited thereto, and various configurations are possible if it can be electrically connected to the external device 20 .
- PIN pin
- the second connection terminal 120 is coupled to the sensing module 200 to be described later and is electrically connectable to the sensing module 200 .
- the second connection terminal 120 may be provided in the form of a micro USB or pin, but is not limited thereto, and various configurations may be used as long as it can be electrically connected to the sensing module 200 .
- the controller 130 is electrically connected to the first connection terminal 110 and the second connection terminal 120, and a signal ( data) may be configured to control the transmission.
- the controller 130 may be electrically connected to the first connection terminal 110 and the second connection terminal 120 through an electric wire, but is not limited thereto. If the two connection terminals 120 can be electrically connected, various configurations may be used.
- the main body 140 includes a lower body in which the first connection terminal 110 , the second connection terminal 120 , and the controller 130 are installed, and is coupled to the lower body to provide the first connection terminal 110 .
- the second connection terminal 120 and an upper body protecting an upper portion of the controller 130 may be included.
- the sensing module 200 detects a target material from an analysis sample introduced therein, and transmits an electrical signal generated by reacting with the sensed target material to the connection module 100 .
- a milliwell or microwell structure 220 is provided as configured to transmit.
- the analysis sample may refer to a solution material, and may refer to a solution diluted with blood, serum, or plasma.
- the analysis sample is not limited thereto, and as long as it is a solution material, it may be a variety of materials.
- the sensing module 200 may include a sensor 210 that detects the target material from the analyte sample, reacts with the target material to generate the electrical signal, and the sensor 210 includes a substrate 211 , a plurality of electrodes 212 , and a milliwell or microwell structure 220 .
- the senor 210 may be provided with a sensing material (eg, an enzyme, etc.) that reacts with the target material included in the analysis sample to detect the target material.
- a sensing material eg, an enzyme, etc.
- the sensor 210 When the sensor 210 comes into contact with the analysis sample, it interacts with a target material included in the analysis sample to generate an electrical signal.
- the external device 20 connected to the biosensor 10 may analyze the electrical signal generated from the biosensor 10 to detect the presence or concentration of the target material.
- the sensor 210 is not limited thereto, and may be configured to move, stop, filter, purify, react, and mix the analysis sample.
- the substrate 211 provided in the sensor 210 may have a predetermined size, and the substrate 211 may have a size of 2 x 2 mm.
- the size of the substrate 211 is not necessarily limited thereto, and may be changed to various sizes.
- the substrate 211 may be made of a material that has elasticity and is bendable.
- the substrate 211 may be a polyurethane (Poly urethane, PU)-based, polydimethylsiloxane (PDMS)-based, NOA (Noland Optical Adhesive)-based, epoxy-based, polyethylene terephthalate, At least one of PET), polymethyl methacrylate (PMMA), polyimide (PI), polystyrene (PS), polyethylene naphthalate (PEN), and polycarbonate (PC) It may be made of a material of PET), polymethyl methacrylate (PMMA), polyimide (PI), polystyrene (PS), polyethylene naphthalate (PEN), and polycarbonate (PC) It may be made of a material of
- the material of the substrate 211 is not necessarily limited thereto, and electrodes generating a potential difference according to a reaction with a target material may be disposed, and various materials having flexibility may be applied.
- the electrode 212 may be provided in plurality, and the electrode 212 may be provided on one surface of the substrate 211 and may react with a target material included in the analysis sample to generate an electrochemical signal. .
- the plurality of electrodes 212 includes a first electrode 212a for oxidation or reduction reaction with a target material, a second electrode 212b for oxidation or reduction reaction with a target material in opposition to the reaction of the first electrode 212a, and a third electrode 212c configured to maintain a constant operating voltage between the first electrode 212a and the second electrode 212b.
- the plurality of electrodes 212 may be formed on the insulating substrate 211 through a screen printing method.
- the second electrode 212b and the third electrode 212c may be disposed to surround the first electrode 212a.
- the first electrode 212a and the second electrode 212b may be disposed on the substrate 211 to be spaced apart from each other by a predetermined distance.
- the first electrode 212a may have a circular shape
- the second electrode 212b may have a semicircular shape to surround a portion of the first electrode 212a.
- the arrangement shape of the first electrode 212a and the second electrode 212b and the shape of each of these electrodes are not limited thereto, and may be changed as necessary.
- the first electrode 212a may refer to a working electrode that performs oxidation or reduction reaction by reaction with a target material or an analyte sample including the target material on the substrate 211 .
- a bioreceptor specifically binding to a target material may be disposed on the first electrode 212a.
- a pillar having a conductive layer deposited thereon is disposed on the first electrode 212a, and a bioreceptor such as an antibody, antigen, or aptamer may be disposed on the pillar.
- a material that reacts with a target material to induce an electrochemical signal may be further disposed on the first electrode 212a.
- the reaction area with the target material is widened, and through this, the biosensor 10 absorbs a small amount of the target material. It can also provide sensitive qualitative and quantitative analysis results.
- the pillar may be a polymer structure having a nano size.
- the pillar may be made of at least one of polyurethane, polydimethylsiloxane, Norland Optical Adhesives (NOA), epoxy, polyethylene terephthalate, polymethyl methacrylate, polyimide, polystyrene, polyethylene naphtharate, polycarbonate, and combinations thereof. have.
- NOA Norland Optical Adhesives
- the pillars may also be made of a combination of polyurethane and NOA (eg NOA 68).
- NOA eg NOA 68
- the pillars are not limited thereto, and may be made of more various polymers as long as they have flexibility.
- the conductive layer deposited on the pillar may refer to a layer made of a conductive material.
- the conductive layer may be formed of at least one of Ni, Zn, Pd, Ag, Cd, Pt, Ga, In, and Au, but is not limited thereto.
- the second electrode 212b may refer to a counter electrode facing the first electrode 212a on the substrate 211 . Accordingly, if an oxidation reaction occurs in the first electrode 212a by reaction with a target material, a reduction reaction may occur in the second electrode 212b.
- the above-described pillars having a conductive layer deposited thereon may be disposed on the second electrode 212b.
- the third electrode 212c may refer to a reference electrode in which a potential is stably maintained even when in contact with a target material.
- a reaction layer capable of maintaining a constant potential even when in contact with a target material may be provided on the third electrode 212c.
- the reaction layer is Ag/AgCl, Ag, Hg2SO4, Ag/Ag+, Hg/Hg2SO4, RE-6H, Hg/HgO, Hg/Hg2Cl2, Ag/Ag2SO4, Cu/CuSO4, KCl saturated calomel half cell (SCE) and salt bridge
- SCE KCl saturated calomel half cell
- the potential difference of platinum may be made of a known material in advance.
- the milliwell or microwell structure 220 is provided on the electrode 212 and includes a plurality of grooves.
- the milliwell or microwell structure 220 may be provided at an inlet into which the analysis sample is inserted into the sensing module 200 , and the resolution is improved by using the milliwell or microwell structure 220 . There are advantages to doing it.
- the milliwell or microwell structure 220 may be provided above a working electrode, and the milliwell or microwell structure 220 includes the first electrode 212a formed of a working electrode. It may be provided on the top.
- the sensing module 200 may further include a connection pad 230 , a connection port 240 , a housing 250 , and a bonding wire 260 .
- connection pads 230 may be provided, and may be electrically connected to the sensor 210 through the bonding wire 260 . 4 and 6,
- a connection pad 213 for electrically connecting the plurality of connection pads 230 and the plurality of electrodes 212 provided in the sensor 210 to each other may be further provided on the bottom surface of the sensor 210 .
- connection pad 213 attached to the bottom surface of the sensor 210 is electrically connected to the plurality of electrodes 212 provided in the sensor 210 , and the other surface of the connection pad 213 is the It may be electrically connected to the plurality of connection pads 230 through the bonding wire 260 .
- the plurality of connection pads 230 and the connection pads 213 may be provided in a copper foil shape, and a surface of the plurality of connection pads 230 may be further coated with gold foil.
- connection pad 213 and the sensor 210 may be electrically connected to each other through a conductive material (not shown), and a double-sided adhesive carbon tape may be used as the conductive material.
- a conductive material is not limited thereto, and various materials may be used as long as the connection pad 213 and the sensor 210 can be electrically connected.
- the bonding wire 260 is made of gold (Au), and one end of the bonding wire 260 is bonded to the connection pad 213 attached to the sensor 210 , and is provided in the sensor 210 .
- the electrodes 212 may be electrically connected, and the other end of the bonding wire 260 may be bonded to the plurality of connection pads 230 to be electrically connected to the plurality of connection pads 230 .
- connection port 240 may be electrically connected to the plurality of connection pads 230 , and may be coupled to the connection module 100 to be electrically connected to the connection module 100 .
- connection port 240 may be electrically connected to the plurality of connection pads 230 through an electric wire 261 , and when coupled to the connection module 100 , the sensor ( Power may be supplied to the 210 , and an electrical signal generated from the sensor 210 may be transmitted to the connection module 100 .
- the connection port 240 may be provided in a shape corresponding to the second connection terminal 120 provided in the connection module 100 .
- the housing 250 includes the sensor 210 , the plurality of connection pads 230 , and the connection port 240 installed therein.
- the housing 250 includes the sensor 210 and the plurality of connection pads ( 230), may be configured to surround the circumference of the connection port 240 to protect the sensor 210, the plurality of connection pads 230, and the connection port 240 from the outside.
- the housing 250 is disposed opposite to the first member 251 and the first member 251 having a channel 253 for guiding the analyte sample provided from the outside to the electrode 212 of the sensor 210 . and is coupled to the first member 251 and includes a second member 252 on which the sensor 210 , the plurality of connection pads 230 , and the connection port 240 are seated.
- the channel 253 is a hole formed through the first member 251 , and the channel 253 is an inclined surface 254 for guiding an analysis sample provided from the outside to the electrode 212 of the sensor 210 . ) is included.
- the inclined surface 254 may be provided along the circumference of the channel 253 , and the analysis sample provided from the outside moves along the inclined surface 254 stably to the electrode 212 of the sensor 210 . be able to reach
- the milliwell or microwell structure 220 may be provided in the channel 253 .
- the milliwell or microwell structure 220 may be provided on the first electrode 212a-working electrode, and the channel 253 is the first electrode 212a-working electrode. (working electrode) may be provided on top.
- the milliwell or microwell structure 220 is preferably provided in the channel 253 , and the milliwell or microwell structure 220 can be supported while being coupled to the channel 253 .
- the milliwell or microwell structure 220 is provided in the channel 253 , it is possible to improve resolution when the analyzed sample is moved to the electrode 212 through the channel 253 .
- the first member 251 may be coupled to the second member 252 to cover the sensor 210 and the connection port 240 disposed on the second member 252 to protect them from the outside. . Also, the first member 251 may press the sensor 210 and the connection port 240 with a predetermined force to firmly support them. In addition, the first member 251 prevents the analysis sample provided to the sensor 210 through the channel 253 from leaking to the outside of the housing 250 through coupling with the second member 252 . can do.
- the sensor 210 , the plurality of connection pads 230 , and the connection port 240 are accommodated between the first member 251 and the second member 252 . and an accommodation space 250a for protecting the bonding wire 260 may be provided.
- the accommodating space 250a may be provided in the form of an intaglio pattern inside the first member 251 and the second member 252, respectively.
- At least one coupling protrusion 252a coupled to the first member 251 and supporting the first member 251 is provided on the second member 252 .
- One member 251 may be provided with at least one coupling groove 251a corresponding to the coupling protrusion 252a.
- the center of the channel 253 and the center of the sensor 210 may be naturally aligned at a predetermined position.
- a sealer made of a rubber or silicone material that seals the space between the coupling protrusion 252a and the coupling groove 251a. may be further provided.
- the second member 252 has a seating groove 252b in which the sensor 210 is seated and supported, and a collection groove 252c in which an analysis sample flowing down from the sensor 210 is stored. This may be further provided.
- a sample guide surface 252d for guiding the analysis sample flowing out of the sensor 210 toward the collection groove 252c may be further provided between the sensor 210 and the collection groove 252c, and the sample guide surface (252d) may be formed in an inclined shape.
- the housing 250 includes a flow state of an analyte sample, leakage of an analyte sample, an alignment state of the channel 253 and the sensor 210 , and the bonding wire 260 through
- the sensor 210 and the plurality of connection pads 230 are connected to each other and the electrical connection between the plurality of connection pads 230 and the connection port 240 can be easily observed from the outside, and it is made of a transparent material. can be
- the housing 250 is made of at least one of polymethyl methacrylate, polycarbonate, cyclic olefin copolymer, polyethylene sulfone, and polystyrene, or at least two or more of them. It may be provided with these combined materials.
- the material of the housing 250 is not necessarily limited thereto, and may be made of a polydimethylsiloxane material, which is a silicone-based organic polymer.
- the biosensor using the milliwell or microwell structure according to an embodiment of the present invention may be modified and used as follows.
- a plurality of sensing modules 200 of the biosensor may be provided, and the plurality of sensing modules 200 are detachably coupled to the connection module 100 .
- the connection module 100 may be electrically connected to the plurality of sensing modules 200 .
- connection module 100 includes the first connection terminal 110 that can be coupled to the external device 20 and a second connection terminal 120 that can be coupled to the sensing module 200, A plurality of 2 connection terminals 120 may be provided.
- the plurality of sensing modules 200 may be selectively coupled to the plurality of second connection terminals 120 .
- the controller 130 is configured to operate through the plurality of second connection terminals 120 . After storing the order in which the electrical signals are transmitted, the electrical signals of the sensing module 200 connected to the corresponding second connection terminal 120 may be sequentially received and transmitted to the first connection terminal 110 .
- the connection module 100 is a power supply unit 150 for controlling the connection between the plurality of second connection terminals 120 and the controller 130 between the plurality of second connection terminals 120 and the controller 130 . may further include.
- a plurality of the power supply unit 150 may be provided, and may be individually connected to the plurality of second connection terminals 120 . Also, the power supply unit 150 may be connected to each of the controllers 130 .
- the power supply unit 150 may be configured to be ON/OFF controlled through a user's operation. Only when the power supply unit 150 is controlled to an ON state through a user's operation, the power supply unit 150 is connected to the second connection terminal 120 . An electrical signal of the connected sensing module 200 may be transmitted to the controller 130 .
- connection module 100 is installed on the periphery of the first connection terminal 110, the angle adjusting unit ( 160) may be further included.
- the angle adjusting unit 160 is coupled to the first connection terminal 110 and is installed on the main body 140 and the rotating member 161 configured to rotate together with the first connection terminal 110 and is provided inside the
- the rotating member 161 may be accommodated and may include a guide member 162 for guiding the rotation of the rotating member 161 .
- a plurality of projections 161a spaced apart from each other are provided on the outer peripheral surface of the rotating member 161, and a plurality of grooves 162a corresponding to the plurality of projections 161a are provided on the inner peripheral surface of the guide member 162.
- the user can arrange the plurality of second connection terminals 120 in predetermined positions by rotating the main body 140 in a state in which the first connection terminal 110 is coupled to the external device 20 . have.
- connection module 100 and the sensing module 200 may be detachably coupled to each other through magnetism, and may be electrically connected to each other through surface contact. .
- connection module 100 may include a first coupling member 170
- the sensing module 200 may include a second coupling member 270 capable of being coupled to the first coupling member 170 .
- the first coupling member 170 includes a coupling protrusion 171 protruding from one end of the connection module 100 and a first magnetic body 172 accommodated at an end of the coupling protrusion 171 .
- the coupling protrusion 171 may be formed in a wedge structure in which the size of the outer diameter gradually decreases along the protrusion direction. Through this, when the connection module 100 and the sensing module 200 are coupled, an inclined outer circumferential surface of the coupling protrusion 171 is a coupling groove of the second coupling member 270 provided in the sensing module 200 . Guided to the inner peripheral surface of the (271) can be easily coupled.
- the second coupling member 270 is formed concavely on the other end of the sensing module 200 facing one end of the connection module 100 , and the coupling groove 271 is formed inside the coupling groove 271 . It is accommodated in the first magnetic body 172 and may include a second magnetic body 272 capable of being coupled through a magnetic force.
- connection module 100 and the sensing module 200 are primarily coupled through the structural coupling of the coupling protrusion 171 and the coupling groove 271, and the first magnetic body 172 and the second As the magnetic body 272 is secondarily coupled through coupling using the magnetism, they may be stably fixed to each other.
- the sensing module 200 has the magnetic force of the second magnetic body 272 shielding the magnetic force of the second magnetic body 272 so as not to affect other parts of the sensing module 200.
- a shielding structure 280 configured to do so may be provided.
- the shielding structure 280 may be provided inside the housing 250 and be provided in a shape surrounding the coupling groove 271 of the second coupling member 270 and the periphery of the second magnetic body 272 . have.
- the shielding structure 280 may be made of the same material as the housing 250 , and may be made of a material capable of shielding magnetic force.
- connection module 100 and the sensing module 200 may be electrically connected to each other through surface contact coupling.
- the second connection terminal 120 of the connection module 100 connected to the connection port 240 of the sensing module 200 is a flat plate type that can be electrically connected to each other through surface contact. It may be provided in the form of a terminal.
- connection port 240 and the second connection terminal 120 can be electrically connected to each other just by being interviewed.
- connection module 100 and the sensing module 200 This makes it easier to combine and separate the connection module 100 and the sensing module 200, and it is possible to prevent the connection port 240 and the second connection terminal 120 from being damaged even in a detachable type.
- An identification film may be attached to the end of the sensing module 200 according to an embodiment of the present invention to confirm whether or not the sensing module 200 is used through the presence or absence of damage.
- a receiving groove (not shown) capable of engaging with a wedge-shaped pressing protrusion (not shown) provided at the end of the connection module 100 is provided, and at the entrance of the receiving groove, the sensing module ( 200) may be attached to the identification film in the form of a thin film configured to block the entrance of the receiving groove.
- the identification film may be configured to be damaged by being pressed with a predetermined force by the pressing protrusion of the connection module 100 inserted into the receiving groove when the sensing module 200 and the connection module 100 are coupled. Accordingly, the user can determine whether the sensing module 200 is used by checking whether the identification film provided at the end of the sensing module 200 is damaged.
- the configuration capable of confirming whether the sensing module 200 is used is not necessarily limited thereto, and may be changed and applied in various forms.
- the sensing module 200 may include a plurality of sensing units configured to detect a target material from an analysis sample.
- the plurality of sensing units are electrically connected to the connection port 240 , and may be installed inside the housing 250 .
- Each of the plurality of sensing units includes the sensor 210 , and the plurality of sensing units are separated from each other through a partition wall 214 , through the connection port 240 electrically coupleable to the connection module 100 . They can be electrically connected to each other.
- the plurality of sensing units may include a first sensing unit 210a and a second sensing unit 210b.
- the first sensing unit 210a and the second sensing unit 210b may be disposed in spaces independent from each other through the partition wall 214 provided in the housing 250 .
- the first sensing unit 210a and the second sensing unit 210b each sense a target material from the contacted analyte, and the sensor 210 and the bonding wire that react with the target material to generate an electrical signal
- the connection pad 230 may be electrically connected to the sensor 210 through 260 and electrically connected to the connection port 240 through the electric wire 261 .
- first sensing unit 210a and the second sensing unit 210b may each include the channel 253 , and each of the channels 253 has the milliwell or microwell structure 220 . may be provided.
- connection pad 230 and the connection port 240 are provided.
- a power supply unit 290 for controlling the connection may be further provided.
- the power supply unit 290 is electrically connected to the connection pad 230 and the connection port 240 , and may be configured to be ON/OFF controlled through a user's manipulation. Accordingly, only when the power supply unit 290 is controlled to be in the ON state through a user's manipulation, the electrical signal of the sensor 210 connected to the connection pad 230 may be transmitted to the connection port 240 .
- the biosensor using the milliwell or microwell structure according to the embodiment of the present invention has been described as including the first sensing unit 210a and the second sensing unit 210b, but is not limited thereto, and 2 More than one sensing unit may be provided.
- the biosensor using the milliwell or microwell structure according to the embodiment of the present invention described above has the following effects.
- the biosensor 10 using a milliwell or microwell structure is configured to be electrically connected to the external device 20 through a connection terminal provided in the form of a USB or pin, so it can be applied to various devices. Accordingly, it is possible to reduce costs, as well as to be easily used in real life, so that convenience and usability of the product can be increased.
- the biosensor 10 using a milliwell or microwell structure includes a connection module 100 electrically connected to an external device 20 , and sensing detachable from the connection module 100 . Since it is composed of the module 200, various types of tests can be performed by replacing the sensing module 200, and only the sensing module 200 can be replaced while the connection module 100 is coupled to the external device 20 Thus, it is possible to perform continuous and rapid inspection.
- the biosensor 10 using a milliwell or microwell structure is configured such that the sensing module 200 and the external device 20 are electrically connected to each other via the connection module 100 . Therefore, even when the biosensor 10 is coupled to or separated from the external device 20 , the sensing portion is prevented from being damaged by the external device 20 .
- the shape of the electrode provided on the substrate 211 applied to the biosensor 10 using the milliwell or microwell structure according to the embodiment of the present invention is improved, a larger number of sensors 210 than in the prior art It is possible to manufacture the , so that the manufacturing cost can be reduced.
- the sensor 210 can be manufactured in a size of 2 x 2 mm. Therefore, the number of sensors 210 that can be manufactured using an existing 8-inch wafer has greatly increased from a low of 1000 to a high of 20,000. Except for the sensor 210, the manufacturing cost can be further reduced by using a low-cost PCB process.
- the biosensor 10 using a milliwell or microwell structure has the advantage of improving resolution by inserting an analysis sample through the milliwell or microwell structure 220 . have.
- connection module 110 ...first connection terminal
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Abstract
The present invention relates to a biosensor using a milliwell or microwell structure, comprising: a connection module that can be electrically connected to an external device; and a sensing module configured to sense a target material from an analysis sample introduced thereinto, and transmit an electrical signal generated by reacting with the sensed target material, to the connection module, wherein: the sensing module has a sensor which senses the target material from the analysis sample, and reacts with the target material to generate the electrical signal; the sensor includes a substrate, a plurality of electrodes provided on one surface of the substrate and reacting with the target material to generate an electrochemical signal, and a milliwell or microwell structure provided on the electrodes and comprising a plurality of grooves; and the sensing module is detachable from the connection module.
Description
본 발명은 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서에 관한 것으로, 더욱 상세하게는 외부장치와 전기적으로 연결 가능한 접속모듈과 접속모듈에 탈부착 가능하면서 밀리웰 또는 마이크로웰 구조가 형성된 센싱모듈을 사용함에 따라 센싱 부분의 교체가 가능한 바이오 센서에 관한 것이다. The present invention relates to a biosensor using a milliwell or microwell structure, and more particularly, a connection module electrically connectable to an external device and a sensing module in which a milliwell or microwell structure is formed while being detachable from the connection module. It relates to a biosensor in which the sensing part can be replaced according to the present invention.
인간의 질병 증세 및 진행, 나아가 식품의 컨디션에 대한 예측을 가능하게 하는, 호르몬, 단백질, 그리고 병원균과 같은 표적 물질들은 항원-항체 반응을 이용한 면역 분석법에 의해 분석될 수 있다. 한편, 면역 분석법에 기초한 표적 물질의 분석은 대게 특별한 기기가 갖추어진 임상 실험실에서 수행되어 왔다. 그러나, 최근 병원이나 응급실의 의료 현장에서의 검사, 그리고 가정에서 자가 진단의 필요성이 증가함에 따라, 전문지식이나 복잡한 과정이 요구되지 않고 분석 시간이 짧은 면역 분석 플랫폼에 대한 개발이 지속적으로 요구되어 왔다.Target substances such as hormones, proteins, and pathogens, which enable prediction of human disease symptoms and progression, and further the condition of food, can be analyzed by immunoassay using antigen-antibody reactions. On the other hand, analysis of target substances based on immunoassay has been usually performed in clinical laboratories equipped with special equipment. However, as the need for self-diagnosis at home and for examinations at medical sites in hospitals or emergency rooms has recently increased, the development of an immunoassay platform that does not require specialized knowledge or complicated processes and has a short analysis time has been continuously required. .
이를 해결하기 위한 방안으로 전기 화학적 분석에 의해 표적 물질에 대한 빠르고 정확한 분석이 가능한 바이오 센서가 등장하였다. 보다 구체적으로, 바이오 센서에 기초한 전기 화학적 분석법은, 바이오 센서 내에 구비된 전극과 분석 시료와의 접촉에 따른 산화 환원 반응에 의해 생성된 전류를 분석함으로써, 분석 시료 내의 표적 물질에 대한 정성 및 정량 분석을 가능하게 한다.As a solution to this problem, a biosensor capable of fast and accurate analysis of a target material by electrochemical analysis has emerged. More specifically, the electrochemical analysis method based on the biosensor analyzes the current generated by the redox reaction according to the contact between the electrode provided in the biosensor and the analyte sample, thereby qualitative and quantitative analysis of the target material in the analyte sample. makes it possible
바이오 센서의 전극 재료로는 전기적 특성이 우수하고 외부환경에 변함이 없는 금속류(예를 들면, 금, 백금, 팔라듐 등)가 이용될 수 있다. 이러한 재질의 전극은, 재료의 특징을 유지하면서 원가 경쟁력을 가지는 스퍼터링이나 전기도금 방식을 통해 얇은 박막 전극 형태로 제작될 수 있다.As the electrode material of the biosensor, metals (eg, gold, platinum, palladium, etc.) having excellent electrical characteristics and not changing in the external environment may be used. An electrode made of such a material may be manufactured in the form of a thin thin film electrode through sputtering or electroplating having cost competitiveness while maintaining the characteristics of the material.
한편, 바이오 센서에 마련된 전극은 두께가 매우 얇기 때문에, 전류량 측정을 위한 측정장비와의 결합 과정 중에 표적 물질을 센싱하는 부위가 손상되어 접속시 단락 현상이 빈번하게 발생할 수 있다. 이를 예방하기 위하여, 종래의 바이오 센서에는 표적 물질을 센싱하는 센싱부와 측정장비와 연결되는 연결부로 구성된 전극이 마련된다.On the other hand, since the electrode provided in the biosensor has a very thin thickness, a portion for sensing a target material may be damaged during a coupling process with a measuring device for measuring the amount of current, and a short circuit may occur frequently during connection. In order to prevent this, a conventional biosensor is provided with an electrode including a sensing unit for sensing a target material and a connection unit connected to a measuring device.
도 1을 참조하면, 종래의 바이오 센서(1)에 마련되는 전극(2)은 표적 물질을 센싱하기 위한 센싱부(2a)와, 센싱부(2a)로부터 소정의 길이로 연장되어 기판(3) 상에서 센싱부(2a)에 비해 더 넓은 영역에 배치되고, 그 단부가 측정장비(4)와 전기적으로 연결되는 연장부(2b)를 포함한다. 상기 전극(2)을 구비한 바이오 센서(1)는 다이싱 공정을 통하여 미리 설정된 크기로 제작될 수 있다. Referring to FIG. 1 , the electrode 2 provided in the conventional biosensor 1 includes a sensing unit 2a for sensing a target material, and a substrate 3 extending from the sensing unit 2a to a predetermined length. It is disposed in a wider area than the sensing unit (2a) on the top, the end includes an extension (2b) that is electrically connected to the measuring device (4). The biosensor 1 having the electrode 2 may be manufactured in a preset size through a dicing process.
그러나 종래의 바이오 센서(1)는 다음과 같은 문제점이 있다. 종래의 바이오 센서(1)에 마련되는 전극(2)의 길이가 불필요하게 길게 형성됨에 따라, 한정된 넓이를 가지는 웨이퍼 상에 적은 수의 전극(2)을 형성할 수밖에 없어서, 생산성이 낮은 문제점이 있었다.However, the conventional biosensor 1 has the following problems. As the length of the electrode 2 provided in the conventional biosensor 1 is unnecessarily long, a small number of electrodes 2 have to be formed on a wafer having a limited area, resulting in low productivity. .
또한, 종래의 바이오 센서(1)는 도 1에 도시된 바와 같이, 측정장비(4)에 전극(2)이 직접 연결되도록 구성된다. 따라서, 다른 종류의 검사를 수행하기 위해서는 측정장비(4)에 연결된 바이오 센서(1)를 모두 교체해야만 하는 불편함이 있었다.In addition, as shown in FIG. 1 , the conventional biosensor 1 is configured such that the electrode 2 is directly connected to the measuring device 4 . Therefore, in order to perform other types of tests, there is an inconvenience of having to replace all the biosensors 1 connected to the measuring equipment 4 .
이와 함께, 바이오 센서(1)의 전극(2)에 연결된 측정장비(4)의 접속부(4a)는 바이오 센서(1)의 상면과 하면을 가압하여 바이오 센서(1)에 고정되는 집게 형태로 형성되므로, 접속부(4a)를 바이오 센서(1)에 연결할 경우, 바이오 센서(1)의 전극(2)이 손상될 여지가 있음은 물론, 접속부(4a) 혹은 바이오 센서(1)에 예상치 못한 외력이 가해질 경우 측정장비(4)의 접속부(4a)가 쉽게 바이오 센서(1)로부터 분리되는 문제점이 있다.At the same time, the connection part 4a of the measuring device 4 connected to the electrode 2 of the biosensor 1 is formed in the form of tongs fixed to the biosensor 1 by pressing the upper and lower surfaces of the biosensor 1 . Therefore, when the connection part 4a is connected to the biosensor 1, there is a possibility that the electrode 2 of the biosensor 1 may be damaged, and an unexpected external force is applied to the connection part 4a or the biosensor 1 When applied, there is a problem in that the connection part 4a of the measuring device 4 is easily separated from the biosensor 1 .
또한, 종래에는 바이오 센서(1)의 반응을 측정하기 위하여 전용 측정장비(4)를 이용하였으나, 가정 혹은 개인이 상술한 전용 측정장비(4)를 구비하여 바이오 센서(1)의 분석을 수행하는 것은 실질적으로 용이하지 못하고, 이에 따라 다양한 환경 혹은 상황에서 실사용이 불가능하여 활용도가 매우 낮은 문제점이 있었다.In addition, in the prior art, a dedicated measuring device 4 was used to measure the response of the biosensor 1 , but a household or individual is equipped with the above-described dedicated measuring device 4 to perform analysis of the biosensor 1 . It is not practically easy, and accordingly, it is impossible to use it in various environments or situations, so there is a problem that the utilization is very low.
본 발명은 상술한 문제점을 해결하기 위한 것으로, 더욱 상세하게는 외부장치와 전기적으로 연결 가능한 접속모듈과 접속모듈에 탈부착 가능하면서 밀리웰 또는 마이크로웰 구조가 형성된 센싱모듈을 사용함에 따라 센싱 부분의 교체가 가능한 바이오 센서에 관한 것이다. The present invention is to solve the above-described problems, and more specifically, a connection module electrically connectable to an external device and a sensing module detachably attached to the connection module and formed with a milliwell or microwell structure. It relates to a possible biosensor.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서는, 외부장치와 전기적으로 연결 가능한 접속모듈; 내부로 유입된 분석 시료로부터 표적 물질을 감지하고, 상기 감지된 표적 물질과 반응하여 발생된 전기적인 신호를 상기 접속모듈에 전송하도록 구성되는 센싱모듈;을 포함하고, 상기 센싱모듈에는, 상기 분석 시료로부터 상기 표적 물질을 감지하고, 상기 표적 물질과 반응하여 상기 전기적인 신호를 발생시키며 센서가 구비되며, 상기 센서는, 기판;과 상기 기판의 일면에 마련되고, 상기 표적 물질과 반응하여 전기 화학적 신호를 발생시키는 복수개의 전극;과 상기 전극 상부에 구비되며, 복수 개의 홈으로 이루어진 밀리웰 또는 마이크로웰 구조;를 포함하며, 상기 센싱모듈은 상기 접속모듈에 탈부착 가능한 것을 특징으로 하는 것이다. A biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes: a connection module electrically connectable to an external device; A sensing module configured to detect a target material from the analyte sample introduced into the interior, and transmit an electrical signal generated by reacting with the sensed target material to the connection module; including, wherein the sensing module includes the analyte sample A sensor is provided to detect the target material from the It includes a plurality of electrodes for generating a ; and a milliwell or microwell structure provided on the electrode and comprising a plurality of grooves, wherein the sensing module is detachable from the connection module.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 전극은, 상기 표적 물질과 산화 반응하는 제1전극과, 상기 표적 물질과 환원 반응하는 제2전극과, 상기 제1전극과 상기 제2전극 사이에 일정한 작동전압이 유지되도록 구성되는 제3전극을 포함할 수 있다. The electrode of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes a first electrode that oxidizes with the target material, and a second electrode that reacts with the target material by reduction; A third electrode configured to maintain a constant operating voltage between the first electrode and the second electrode may be included.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 밀리웰 또는 마이크로웰 구조는, 상기 제1전극 상부에 구비될 수 있다. The milliwell or microwell structure of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems may be provided on the first electrode.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 접속모듈은,상기 외부장치에 결합 가능한 제1접속단자와, 상기 센싱모듈에 결합 가능한 제2접속단자와, 상기 제1접속단자와 상기 제2접속단자 사이에서 신호의 전송을 제어하도록 구성되는 컨트롤러와, 상기 제1접속단자, 상기 제2접속단자 및 상기 컨트롤러가 설치되는 본체를 포함할 수 있다. The connection module of the biosensor using the milliwell or microwell structure of the present invention for solving the above-described problems includes a first connection terminal that can be coupled to the external device, a second connection terminal that can be coupled to the sensing module and , a controller configured to control transmission of a signal between the first connection terminal and the second connection terminal, and a main body in which the first connection terminal, the second connection terminal, and the controller are installed.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 센싱모듈은, 본딩 와이어를 통하여 상기 센서와 전기적으로 연결되는 접속패드와 상기 접속패드와 전기적으로 연결되고, 상기 접속모듈에 결합 가능한 접속포트와 상기 센서, 상기 접속패드 및 상기 접속포트가 설치되는 하우징을 더 포함할 수 있다. The sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems is electrically connected to a connection pad electrically connected to the sensor and the connection pad through a bonding wire, It may further include a housing in which a connection port coupleable to the connection module, the sensor, the connection pad, and the connection port are installed.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 하우징은, 상기 분석 시료를 상기 센서의 상기 전극으로 안내하는 채널이 구비된 제1부재와, 상기 제1부재에 대향 배치되어 제1부재에 결합되면서 상기 센서, 상기 접속패드, 상기 접속포트가 안착되는 제2부재를 포함하며, 상기 밀리웰 또는 마이크로웰 구조는 상기 채널에 구비될 수 있다. The housing of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems includes a first member having a channel for guiding the analyte sample to the electrode of the sensor, and the first and a second member disposed opposite to the member and coupled to the first member on which the sensor, the connection pad, and the connection port are seated, and the milliwell or microwell structure may be provided in the channel.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 접속모듈과 상기 센싱모듈은, 자성을 통해 서로 탈부착 가능하게 결합되며, 면접촉을 통해 서로 전기적으로 연결될 수 있다. The connection module and the sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems are detachably coupled to each other through magnetism, and can be electrically connected to each other through surface contact. have.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 센싱모듈은 복수 개가 구비되면서 상기 접속모듈에 탈부착 가능하게 결합되며, 상기 접속모듈은, 상기 외부장치와 결합 가능한 제1접속단자와, 상기 센싱모듈에 결합 가능한 제2접속단자를 포함하며, 상기 제1접속단자와 상기 제2접속단자 사이에서 신호의 전송을 제어하도록 구성되는 컨트롤러와, 상기 제1접속단자, 상기 제2접속단자 및 상기 컨트롤러가 설치되는 본체를 포함하며, 상기 제2접속단자는 복수 개가 구비될 수 있다. A plurality of the sensing modules of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems are provided and detachably coupled to the connection module, and the connection module is coupled to the external device A controller comprising a first possible connection terminal and a second connection terminal capable of being coupled to the sensing module, the controller configured to control transmission of a signal between the first connection terminal and the second connection terminal, and the first connection terminal , and a main body in which the second connection terminal and the controller are installed, and a plurality of the second connection terminals may be provided.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 접속모듈은, 상기 제2접속단자와 상기 컨트롤러 사이의 접속을 제어하는 전원부를 포함할 수 있다. The connection module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems may include a power supply for controlling the connection between the second connection terminal and the controller.
상술한 문제점을 해결하기 위한 본 발명의 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서의 상기 센싱모듈에는, 상기 센서를 포함하는 센싱부가 복수 개 구비되며, 복수 개의 상기 센싱부는 격벽을 통해 서로 분리되되, 상기 접속모듈과 전기적으로 결합 가능한 접속포트를 통해 서로 전기적으로 연결될 수 있다. The sensing module of the biosensor using the milliwell or microwell structure of the present invention for solving the above problems is provided with a plurality of sensing units including the sensors, and the plurality of sensing units are separated from each other through a partition wall. , may be electrically connected to each other through a connection port electrically coupleable to the connection module.
본 발명은 USB 또는 핀 형태로 마련된 접속단자를 통해 바이오 센서와 외부장치를 전기적으로 연결되도록 구성되므로, 다양한 장치에 적용 가능하여 비용을 절감할 수 있음은 물론, 실생활에서도 쉽게 사용 가능하여 편의성 및 제품의 활용성이 증대될 수 있는 장점이 있다. Since the present invention is configured to electrically connect a biosensor and an external device through a connection terminal provided in the form of a USB or pin, it can be applied to a variety of devices, thereby reducing costs, as well as being easily usable in real life for convenience and product It has the advantage of increasing the usability of
또한, 본 발명은 밀리웰 또는 마이크로웰 구조를 통해 분석 시료를 삽입함에 따라 분해능을 향상시킬 수 있는 장점이 있으며, 본 발명의 바이오 센서는 외부장치와 전기적으로 연결되는 접속모듈과, 접속모듈에 탈착 가능한 센싱모듈로 구성되므로, 센싱모듈의 교체를 통해 다양한 종류의 검사를 수행 가능하고, 접속모듈이 외부장치에 결합된 상태에서 센싱모듈만 교체 가능하여 연속적으로 신속한 검사를 수행할 수 있는 장점이 있다. In addition, the present invention has an advantage in that the resolution can be improved by inserting an analyte sample through a milliwell or microwell structure, and the biosensor of the present invention includes a connection module electrically connected to an external device and detachable from the connection module Since it is composed of possible sensing modules, various types of inspections can be performed by replacing the sensing module, and only the sensing module can be replaced while the connection module is coupled to an external device, so it has the advantage of continuously and rapidly performing inspections. .
또한, 본 발명은 접속모듈을 매개로 센싱모듈과 외부장치가 서로 전기적으로 연결되도록 구성되므로, 바이오 센서를 외부장치에 결합하거나 분리할 경우에도 센싱부위가 외부장치에 의해 손상되는 것이 방지되는 장점이 있다. In addition, since the present invention is configured so that the sensing module and the external device are electrically connected to each other through the connection module, the sensing part is prevented from being damaged by the external device even when the biosensor is coupled to or separated from the external device. have.
이와 함께, 본 발명은 기판에 마련되는 전극의 형상이 개선됨으로써, 종래 대비 더 많은 수량의 바이오 센서의 제작이 가능하게 되어, 제조비용이 절감될 수 있는 장점이 있다. In addition, the present invention has an advantage in that the shape of the electrode provided on the substrate is improved, so that a larger number of biosensors can be manufactured compared to the prior art, thereby reducing the manufacturing cost.
도 1은 종래의 바이오 센서를 개략적으로 나타낸 도면이다.1 is a diagram schematically showing a conventional biosensor.
도 2는 본 발명의 실시예에 따른 바이오 센서가 외부장치에 연결된 상태를 나타내는 사용상태도이다:2 is a state diagram showing a state in which a biosensor according to an embodiment of the present invention is connected to an external device:
도 2a는 스마트폰 혹은 태블릿 형태의 외부장치와 바이오 센서가 연결된 상태를 나타낸 사용상태도이고; 도 2b는 PC 형태의 외부장치와 바이오 센서가 연결된 상태를 나타낸 사용상태도이다.2A is a state diagram showing a state in which an external device in the form of a smartphone or tablet and a biosensor are connected; 2B is a state diagram showing a state in which an external device in the form of a PC and a biosensor are connected.
도 3은 본 발명의 일 실시예에 따라 밀리웰 또는 마이크로웰 구조가 형성된 바이오 센서를 나타낸 사시도이다.3 is a perspective view illustrating a biosensor in which a milliwell or microwell structure is formed according to an embodiment of the present invention.
도 4는 본 발명의 일 실시예에 따른 바이오 센서가 분해된 상태를 나타낸 평면도이다.4 is a plan view illustrating a disassembled state of a biosensor according to an embodiment of the present invention.
도 5는 도 4의 VI-VI 선 단면도이다.FIG. 5 is a cross-sectional view taken along line VI-VI of FIG. 4 .
도 6은 도 4의 “A”부분을 나타낸 측면도이다.6 is a side view showing a portion “A” of FIG. 4 .
도 7은 본 발명의 다른 실시예에 따른 바이오 센서가 분해된 상태를 개략적으로 나타낸 평면도이다.7 is a plan view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
도 8은 도 7의 “B”부분을 확대한 확대도이다.FIG. 8 is an enlarged view of part “B” of FIG. 7 .
도 9는 본 발명의 또 다른 실시예에 따른 바이오 센서가 분해된 상태를 개략적으로 나타낸 평면도이다.9 is a plan view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
도 10은 도 9의 “C”부분이 결합되는 과정을 개략적으로 나타낸 도면이다.FIG. 10 is a diagram schematically illustrating a process in which part “C” of FIG. 9 is coupled.
도 11은 본 발명의 또 다른 실시예에 따른 바이오 센서가 분해된 상태를 개략적으로 나타낸 측면도이다.11 is a side view schematically illustrating a state in which a biosensor according to another embodiment of the present invention is disassembled.
본 명세서는 본 발명의 권리범위를 명확히 하고, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 본 발명을 실시할 수 있도록, 본 발명의 원리를 설명하고, 실시예들을 개시한다. 개시된 실시예들은 다양한 형태로 구현될 수 있다.This specification clarifies the scope of the present invention, explains the principles of the present invention, and discloses embodiments so that those of ordinary skill in the art to which the present invention pertains can practice the present invention. The disclosed embodiments may be implemented in various forms.
본 발명의 다양한 실시예에서 사용될 수 있는 "포함한다" 또는 "포함할 수 있다" 등의 표현은 발명(disclosure)된 해당 기능, 동작 또는 구성요소 등의 존재를 가리키며, 추가적인 하나 이상의 기능, 동작 또는 구성요소 등을 제한하지 않는다. 또한, 본 발명의 다양한 실시예에서, "포함하다" 또는 "가지다" 등의 용어는 명세서상에 기재된 특징, 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것이 존재함을 지정하려는 것이지, 하나 또는 그 이상의 다른 특징들이나 숫자, 단계, 동작, 구성요소, 부품 또는 이들을 조합한 것들의 존재 또는 부가 가능성을 미리 배제하지 않는 것으로 이해되어야 한다.Expressions such as “comprises” or “may include” that may be used in various embodiments of the present invention indicate the existence of the disclosed function, operation, or component, and may include one or more additional functions, operations, or components, etc. are not limited. In addition, in various embodiments of the present invention, terms such as "comprise" or "have" are intended to designate that a feature, number, step, action, component, part, or combination thereof described in the specification is present, It should be understood that it does not preclude the possibility of addition or existence of one or more other features or numbers, steps, operations, components, parts, or combinations thereof.
제1, 제2 등과 같이 서수를 포함하는 용어는 다양한 구성요소들을 설명하는데 사용될 수 있지만, 이러한 구성요소들은 상술한 용어에 의해 한정되지는 않는다. 상술한 용어는 하나의 구성요소를 다른 구성요소로부터 구별하는 목적으로만 사용된다.Terms including an ordinal number, such as first, second, etc., may be used to describe various elements, but these elements are not limited by the above-described terms. The above terminology is used only for the purpose of distinguishing one component from another component.
어떤 구성요소가 다른 구성요소에 "연결되어, 결합되어" 있다고 언급된 때에는, 상기 어떤 구성요소가 상기 다른 구성요소에 직접적으로 연결 또는 결합되어 있을 수도 있지만, 상기 어떤 구성요소와 상기 다른 구성요소 사이에 새로운 다른 구성요소가 존재할 수도 있다고 이해되어야 할 것이다. 반면에, 어떤 구성요소가 다른 구성요소에 "직접 연결되어" 있다거나 "직접 결합되어" 있다고 언급된 때에는, 상기 어떤 구성요소와 상기 다른 구성요소 사이에 새로운 다른 구성요소가 존재하지 않는 것으로 이해될 수 있어야 할 것이다.When a component is referred to as being “connected to and coupled to” another component, the component may be directly connected or coupled to the other component, but between the component and the other component. It should be understood that there may be other new components in the On the other hand, when it is said that an element is "directly connected" or "directly coupled" to another element, it will be understood that no new element exists between the element and the other element. should be able to
한편, 본 명세서에서 사용되는 구성요소에 대한 "모듈" 또는 "부"는 적어도 하나의 기능 또는 동작을 수행한다. 그리고, "모듈" 또는 "부"는 하드웨어, 소프트웨어 또는 하드웨어와 소프트웨어의 조합에 의해 기능 또는 동작을 수행할 수 있다. 또한, 특정 하드웨어에서 수행되어야 하거나 적어도 하나의 프로세서에서 수행되는 "모듈" 또는 "부"를 제외한 복수의 "모듈들" 또는 복수의 "부들"은 적어도 하나의 모듈로 통합될 수도 있다. 단수의 표현은 문맥상 명백하게 다르게 뜻하지 않는 한, 복수의 표현을 포함한다.Meanwhile, as used herein, a “module” or “unit” for a component performs at least one function or operation. In addition, a “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software. In addition, a plurality of “modules” or a plurality of “units” other than a “module” or “unit” that must be performed in specific hardware or are executed in at least one processor may be integrated into at least one module. The singular expression includes the plural expression unless the context clearly dictates otherwise.
그 밖에도, 본 발명을 설명함에 있어서, 관련된 공지 기능 혹은 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우, 그에 대한 상세한 설명은 축약하거나 생략한다.In addition, in describing the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be abbreviated or omitted.
본 발명은 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서에 관한 것으로, 외부장치와 전기적으로 연결 가능한 접속모듈과 접속모듈에 탈부착 가능하면서 밀리웰 또는 마이크로웰 구조가 형성된 센싱모듈을 사용함에 따라 센싱 부분의 교체가 가능한 바이오 센서에 관한 것이다. 이하, 첨부된 도면을 참조하여 본 발명의 바람직할 실시예를 상세하게 설명하기로 한다. The present invention relates to a biosensor using a milliwell or microwell structure, and by using a connection module electrically connectable to an external device, and a sensing module detachably attached to the connection module and having a milliwell or microwell structure, the sensing part It relates to a biosensor that can be replaced. Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
도 2를 참조하면, 본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서(10)는 밀리웰 또는 마이크로웰 구조(220)를 사용하여 분해능을 향상시키며, 바이오 센서(10)에서 발생된 전기적 신호를 측정 및 분석 가능한 소프트웨어가 설치된 외부장치(20)와 전기적으로 연결되도록 구성된다. Referring to FIG. 2 , a biosensor 10 using a milliwell or microwell structure according to an embodiment of the present invention uses a milliwell or microwell structure 220 to improve resolution, and the biosensor 10 uses a milliwell or microwell structure 220 . It is configured to be electrically connected to the external device 20 installed with software capable of measuring and analyzing the electrical signal generated in the .
구체적으로, 상기 바이오 센서(10)는 도 2(a)와 같이 스마트폰 혹은 태블릿 형태의 외부장치(20)와 전기적으로 연결되거나, 도 2(b)와 같이 PC 형태의 외부장치(20)와 전기적으로 연결될 수 있다. 다만, 외부장치(20)는 이에 한정되는 것은 아니며, 상기 바이오 센서(10)와 전기적으로 연결 가능한 다양한 형태로 변경되어 사용될 수 있다. Specifically, the biosensor 10 is electrically connected to the external device 20 in the form of a smartphone or tablet as shown in FIG. can be electrically connected. However, the external device 20 is not limited thereto, and may be used in various forms electrically connectable to the biosensor 10 .
상기 외부장치(20)에는 후술할 상기 바이오 센서(10)의 접속모듈(100)과 전기적으로 연결 가능하도록 상기 접속모듈(100)에 마련된 제1접속단자(110)와 결합 가능한 단자부(미도시)가 구비될 수 있다. 또한, 상기 외부장치(20)에는 상기 바이오 센서(10)와 연결시 상기 바이오 센서(10)의 산화 환원 반응에 따른 전위를 측정 및 분석하여 표적 물질에 대한 정성 및 정량 분석이 가능할 수 있는 프로그램 혹은 어플리케이션 등이 설치될 수 있다. 이를 통해, 상기 외부장치(20)는 상기 바이오 센서(10)에서 발생되는 신호를 수집 및 분석하고, 이를 통해 각종 질병 또는 질환 등을 검출하거나, 이들의 변화를 측정하여 디스플레이 할 수 있다.The external device 20 has a terminal unit (not shown) that can be coupled to the first connection terminal 110 provided in the connection module 100 so as to be electrically connectable to the connection module 100 of the biosensor 10 to be described later. may be provided. In addition, when the external device 20 is connected to the biosensor 10, a program or a program capable of performing qualitative and quantitative analysis on a target material by measuring and analyzing the potential according to the redox reaction of the biosensor 10; Applications and the like may be installed. Through this, the external device 20 collects and analyzes signals generated by the biosensor 10, and can detect various diseases or diseases, or measure and display changes thereof.
또한, 상기 외부장치(20)는 유선 또는 무선통신을 통해 인터넷에 연결되어 상기 바이오 센서(10)를 통해 측정된 데이터를 사용자 단말 혹은 의료기관의 서버에 전송할 수 있다. 전송된 데이터를 통해, 검사대상자 또는 의료기관에서는 실시간으로 건강 상태를 모니터링 할 수 있다.In addition, the external device 20 may be connected to the Internet through wired or wireless communication and transmit data measured through the biosensor 10 to a user terminal or a server of a medical institution. Through the transmitted data, the subject or medical institution can monitor the health status in real time.
가령, 예시적으로, 상기 외부장치(20)는 바이오 센서(10)가 결합되어 상기 바이오 센서(10)와 전기적으로 연결되는 입력부(미도시), 입력부를 통하여 전송된 신호(데이터)를 저장, 분석 및 진단하는 제어부(미도시), 제어부로부터 전송받은 데이터를 외부에서 식별 가능한 형태로 출력하는 디스플레이부(미도시), 제어부로부터 전송받은 데이터를 선택된 사용자 단말 및 의료기관 서버로 전송하는 통신부(미도시) 및 입력부, 제어부, 디스플레이부 및 통신부에 전원을 공급하는 전원공급부(미도시) 등을 포함할 수 있다.For example, for example, the external device 20 includes an input unit (not shown) electrically connected to the biosensor 10 to which the biosensor 10 is coupled, a signal (data) transmitted through the input unit, A control unit (not shown) for analyzing and diagnosing, a display unit (not shown) for outputting data transmitted from the control unit in an externally identifiable form, and a communication unit (not shown) for transmitting data received from the control unit to a selected user terminal and a medical institution server ) and a power supply unit (not shown) for supplying power to an input unit, a control unit, a display unit, and a communication unit.
본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서는, 접속모듈(100), 센싱모듈(200), 센서(210)를 포함한다. 이하에서는, 본 발명의 바람직한 실시예를 상세하게 설명하기로 한다. The biosensor using a milliwell or microwell structure according to an embodiment of the present invention includes a connection module 100 , a sensing module 200 , and a sensor 210 . Hereinafter, preferred embodiments of the present invention will be described in detail.
도 3 및 도 4를 참조하면, 상기 접속모듈(100)은 상기 외부장치(20)와 전기적으로 연결 가능한 것이다. 이를 위해, 상기 접속모듈(100)은 제1접속단자(110), 제2접속단자(120), 컨트롤러(130), 본체(140)를 포함한다. 3 and 4 , the connection module 100 is electrically connectable to the external device 20 . To this end, the connection module 100 includes a first connection terminal 110 , a second connection terminal 120 , a controller 130 , and a body 140 .
상기 제1접속단자(110)는 상기 외부장치(20)에 결합되어 상기 외부장치(20)와 전기적으로 연결 가능한 것이다. 상기 제1접속단자(110)는 상기 외부장치(20)와 전기적으로 연결될 수 있다면 다양한 구성이 사용될 수 있다. The first connection terminal 110 is coupled to the external device 20 to be electrically connectable to the external device 20 . The first connection terminal 110 may have various configurations as long as it can be electrically connected to the external device 20 .
상기 제1접속단자(110)는 상기 외부장치(20)에 마련된 상기 단자부에 결합되어 상기 외부장치(20)와 전기적인 접속을 수행할 수 있도록 상기 외부장치(20)의 단자부에 대응되는 형태로 이루어질 수 있다. 구체적으로, 상기 제1접속단자(110)는 USB, 마이크로 USB 혹은 핀(PIN)의 형태로 마련될 수 있으나, 이에 한정되는 것은 아니며, 상기 외부장치(20)에 전기적으로 연결될 수 있다면 다양한 구성이 사용될 수 있다. The first connection terminal 110 is coupled to the terminal provided in the external device 20 in a shape corresponding to the terminal of the external device 20 so as to be electrically connected to the external device 20 . can be done Specifically, the first connection terminal 110 may be provided in the form of a USB, micro USB, or pin (PIN), but is not limited thereto, and various configurations are possible if it can be electrically connected to the external device 20 . can be used
상기 제2접속단자(120)는 후술할 상기 센싱모듈(200)에 결합되어 상기 센싱모듈(200)과 전기적으로 연결 가능한 것이다. 상기 제2접속단자(120)는 마이크로 USB 혹은 핀(PIN)의 형태로 마련될 수 있으나, 이에 한정되는 것은 아니며, 상기 센싱모듈(200)과 전기적으로 연결될 수 있다면 다양한 구성이 사용될 수 있다. The second connection terminal 120 is coupled to the sensing module 200 to be described later and is electrically connectable to the sensing module 200 . The second connection terminal 120 may be provided in the form of a micro USB or pin, but is not limited thereto, and various configurations may be used as long as it can be electrically connected to the sensing module 200 .
상기 컨트롤러(130)는 상기 제1접속단자(110) 및 상기 제2접속단자(120)와 전기적으로 연결되고, 상기 제1접속단자(110)와 상기 제2접속단자(120) 사이에서 신호(데이터)의 전송을 제어하도록 구성될 수 있다. 상기 컨트롤러(130)는 전선을 통하여 상기 제1접속단자(110) 및 상기 제2접속단자(120)와 전기적으로 연결할 수 있으나, 이에 한정되는 것은 아니며, 상기 제1접속단자(110)와 상기 제2접속단자(120)를 전기적으로 연결할 수 있다면 다양한 구성이 사용될 수 있다. The controller 130 is electrically connected to the first connection terminal 110 and the second connection terminal 120, and a signal ( data) may be configured to control the transmission. The controller 130 may be electrically connected to the first connection terminal 110 and the second connection terminal 120 through an electric wire, but is not limited thereto. If the two connection terminals 120 can be electrically connected, various configurations may be used.
상기 본체(140)는 상기 제1접속단자(110), 상기 제2접속단자(120), 상기 컨트롤러(130)가 설치되는 하부체와, 상기 하부체에 결합되어 상기 제1접속단자(110), 상기 제2접속단자(120), 상기 컨트롤러(130)의 상부를 보호하는 상부체를 포함할 수 있다. The main body 140 includes a lower body in which the first connection terminal 110 , the second connection terminal 120 , and the controller 130 are installed, and is coupled to the lower body to provide the first connection terminal 110 . , the second connection terminal 120 , and an upper body protecting an upper portion of the controller 130 may be included.
도 3 및 도 4를 참조하면, 상기 센싱모듈(200)은 내부로 유입된 분석 시료로부터 표적 물질을 감지하고, 상기 감지된 표적 물질과 반응하여 발생된 전기적인 신호를 상기 접속모듈(100)에 전송하도록 구성되면서, 밀리웰 또는 마이크로웰 구조(220)가 구비된 것이다. 3 and 4 , the sensing module 200 detects a target material from an analysis sample introduced therein, and transmits an electrical signal generated by reacting with the sensed target material to the connection module 100 . As configured to transmit, a milliwell or microwell structure 220 is provided.
상기 분석 시료는 용액성 물질을 의미할 수 있으며, 혈액, 혈청 또는 혈장에 의해 희석된 용액을 의미할 수 있다. 다만, 상기 분석 시료는 이에 한정되는 것은 아니며, 용액성 물질이라면 다양한 물질이 될 수 있다. The analysis sample may refer to a solution material, and may refer to a solution diluted with blood, serum, or plasma. However, the analysis sample is not limited thereto, and as long as it is a solution material, it may be a variety of materials.
상기 센싱모듈(200)에는, 상기 분석 시료로부터 상기 표적 물질을 감지하고, 상기 표적 물질과 반응하여 상기 전기적인 신호를 발생시키며 센서(210)가 구비될 수 있으며, 상기 센서(210)는, 기판(211)과 복수 개의 전극(212)과 밀리웰 또는 마이크로웰 구조(220)를 포함한다. The sensing module 200 may include a sensor 210 that detects the target material from the analyte sample, reacts with the target material to generate the electrical signal, and the sensor 210 includes a substrate 211 , a plurality of electrodes 212 , and a milliwell or microwell structure 220 .
구체적으로, 상기 센서(210)에는 분석 시료에 포함된 표적 물질과 반응하여 표적 물질을 감지하는 감지 물질(예컨대, 효소 등)이 마련될 수 있다. 센서(210)는, 분석 시료와 접촉하게 될 경우, 분석 시료에 포함된 표적 물질과 상호 반응하여 전기적인 신호를 발생시킨다. Specifically, the sensor 210 may be provided with a sensing material (eg, an enzyme, etc.) that reacts with the target material included in the analysis sample to detect the target material. When the sensor 210 comes into contact with the analysis sample, it interacts with a target material included in the analysis sample to generate an electrical signal.
이에 따라 상기 바이오 센서(10)와 연결된 외부장치(20)는 상기 바이오 센서(10)에서 발생되는 전기적인 신호를 분석하여 표적 물질의 존재 또는 농도 등을 검출할 수 있다. 다만, 상기 센서(210)는 이에 한정되는 것은 아니며, 분석 시료의 이동, 정지, 여과, 정화, 반응 및 혼합이 이루어질 수 있도록 구성될 수 있다.Accordingly, the external device 20 connected to the biosensor 10 may analyze the electrical signal generated from the biosensor 10 to detect the presence or concentration of the target material. However, the sensor 210 is not limited thereto, and may be configured to move, stop, filter, purify, react, and mix the analysis sample.
상기 센서(210)에 구비된 상기 기판(211)은 미리 지정된 크기를 가질 수 있는 것으로, 상기 기판(211)은 2 x 2 mm의 크기를 가질 수 있다. 그러나 상기 기판(211)의 크기는 반드시 이에 한정되는 것은 아니며, 다양한 크기로 변경될 수 있다.The substrate 211 provided in the sensor 210 may have a predetermined size, and the substrate 211 may have a size of 2 x 2 mm. However, the size of the substrate 211 is not necessarily limited thereto, and may be changed to various sizes.
또한, 상기 기판(211)은 신축성을 가지며, 절곡 가능한 재질로 마련될 수 있다. 가령, 상기 기판(211)은 폴리우레탄(Poly urethane, PU) 계, 폴리디메틸실록산(Polydimethylsiloxane, PDMS) 계, NOA(Noland Optical Adhesive) 계, 에폭시(Epoxy) 계, 폴리에틸렌 테레프타레이트(Polyethylene terephthalate, PET), 폴리메칠메타크릴레이트(Polymethyl methacrylate, PMMA), 폴리이미드(Polyimide, PI), 폴리스티렌(Polystyrene, PS), 폴리에틸렌 나프타레이트(Polyethylene naphthalate, PEN) 및 폴리카보네이트(Polycarbonate, PC) 중 적어도 하나의 재질로 이루어질 수 있다.In addition, the substrate 211 may be made of a material that has elasticity and is bendable. For example, the substrate 211 may be a polyurethane (Poly urethane, PU)-based, polydimethylsiloxane (PDMS)-based, NOA (Noland Optical Adhesive)-based, epoxy-based, polyethylene terephthalate, At least one of PET), polymethyl methacrylate (PMMA), polyimide (PI), polystyrene (PS), polyethylene naphthalate (PEN), and polycarbonate (PC) It may be made of a material of
다만, 상기 기판(211)의 재질은 반드시 이에 제한되는 것이 아니며, 표적 물질과의 반응에 따른 전위차가 발생하는 전극들이 배치될 수 있고, 유연성을 가지는 다양한 소재로 변경되어 적용될 수 있다.However, the material of the substrate 211 is not necessarily limited thereto, and electrodes generating a potential difference according to a reaction with a target material may be disposed, and various materials having flexibility may be applied.
상기 전극(212)은 복수 개가 구비될 수 있는 것으로, 상기 전극(212)은 상기 기판(211)의 일면에 구비되고, 분석 시료에 포함된 표적 물질과 반응하여 전기 화학적 신호를 발생시킬 수 있는 것이다. The electrode 212 may be provided in plurality, and the electrode 212 may be provided on one surface of the substrate 211 and may react with a target material included in the analysis sample to generate an electrochemical signal. .
복수 개의 상기 전극(212)은 표적 물질과 산화 또는 환원 반응하는 제1전극(212a), 상기 제1전극(212a)의 반응에 대향하여 표적 물질과 산화 또는 환원 반응하는 제2전극(212b), 및 상기 제1전극(212a)과 제2전극(212b) 사이에 일정한 작동전압이 유지되도록 구성되는 제3전극(212c)을 포함할 수 있다.The plurality of electrodes 212 includes a first electrode 212a for oxidation or reduction reaction with a target material, a second electrode 212b for oxidation or reduction reaction with a target material in opposition to the reaction of the first electrode 212a, and a third electrode 212c configured to maintain a constant operating voltage between the first electrode 212a and the second electrode 212b.
복수 개의 상기 전극(212)은 절연성 상기 기판(211) 위에 스크린 프린팅 방식을 통해 형성될 수 있다. 상기 제2전극(212b) 및 상기 제3전극(212c)은 상기 제1전극(212a)의 주위를 둘러싸도록 배치될 수 있다. The plurality of electrodes 212 may be formed on the insulating substrate 211 through a screen printing method. The second electrode 212b and the third electrode 212c may be disposed to surround the first electrode 212a.
상기 제1전극(212a)과 상기 제2전극(212b)은 상기 기판(211) 상에서 서로 일정한 거리로 이격된 상태로 배치될 수 있다. 상기 제1전극(212a)은 원형의 형태를 갖고, 상기 제2전극(212b)은 상기 제1전극(212a)의 일부분을 감싸도록 반원의 형태를 가질 수 있다. 다만, 상기 제1 전극(212a) 및 상기 제2전극(212b)의 배치 형태, 및 이들 전극 각각의 모양은 이에 제한되는 것은 아니며, 필요에 따라 변경될 수 있다. The first electrode 212a and the second electrode 212b may be disposed on the substrate 211 to be spaced apart from each other by a predetermined distance. The first electrode 212a may have a circular shape, and the second electrode 212b may have a semicircular shape to surround a portion of the first electrode 212a. However, the arrangement shape of the first electrode 212a and the second electrode 212b and the shape of each of these electrodes are not limited thereto, and may be changed as necessary.
상기 제1전극(212a)은 상기 기판(211) 상에서 표적 물질 또는 표적 물질을 포함하는 분석 시료와의 반응에 의해 산화 또는 환원 반응을 수행하는 작업 전극(working electrode)을 의미할 수 있다. The first electrode 212a may refer to a working electrode that performs oxidation or reduction reaction by reaction with a target material or an analyte sample including the target material on the substrate 211 .
상기 제1전극(212a) 상에는 표적 물질에 특이적으로 결합하는 바이오 리셉터(미도시)가 배치될 수 있다. 상기 제1전극(212a) 상에는 도전층이 증착된 필라(pillar)가 배치되고, 필라에는 항체, 항원, 앱타머 등의 바이오 리셉터가 배치될 수 있다. 상기 제1전극(212a) 상에는 표적 물질과 반응하여 전기 화학적 신호를 유도하는 물질이 더 배치될 수도 있다.A bioreceptor (not shown) specifically binding to a target material may be disposed on the first electrode 212a. A pillar having a conductive layer deposited thereon is disposed on the first electrode 212a, and a bioreceptor such as an antibody, antigen, or aptamer may be disposed on the pillar. A material that reacts with a target material to induce an electrochemical signal may be further disposed on the first electrode 212a.
상기 제1전극(212a)과 상기 제2전극(212b)에 배치된 필라 및 바이오 리셉터를 통하여, 표적 물질과의 반응 면적이 넓어지게 되고, 이를 통해 상기 바이오 센서(10)는 소량의 표적 물질에 대해서도 민감도 높은 정성 및 정량 분석 결과를 제공할 수 있다. 여기서, 필라는 나노 크기를 가지는 폴리머 구조물일 수 있다.Through the pillars and bioreceptors disposed on the first electrode 212a and the second electrode 212b, the reaction area with the target material is widened, and through this, the biosensor 10 absorbs a small amount of the target material. It can also provide sensitive qualitative and quantitative analysis results. Here, the pillar may be a polymer structure having a nano size.
필라는, 폴리우레탄, 폴리디메틸실록산, NOA(Norland Optical Adhesives), 에폭시, 폴리에틸렌 테레프타레이트, 폴리메칠메타크릴레이트, 폴리이미드, 폴리스티렌, 폴리에틸렌 나프타레이트, 폴리카보네이트 및 이들의 조합 중 적어도 하나로 이루어질 수 있다.The pillar may be made of at least one of polyurethane, polydimethylsiloxane, Norland Optical Adhesives (NOA), epoxy, polyethylene terephthalate, polymethyl methacrylate, polyimide, polystyrene, polyethylene naphtharate, polycarbonate, and combinations thereof. have.
또한, 필라는 폴리우레탄과 NOA(예컨대, NOA 68)의 조합으로 이루어질 수도 있다. 그러나, 필라는 이에 제한되는 것은 아니며, 유연성을 갖는 한 보다 다양한 고분자로 이루어질 수 있다.The pillars may also be made of a combination of polyurethane and NOA (eg NOA 68). However, the pillars are not limited thereto, and may be made of more various polymers as long as they have flexibility.
그리고, 필라에 증착된 도전층은 도전성 물질로 구성된 레이어를 의미할 수 있다. 도전층은 Ni, Zn, Pd, Ag, Cd, Pt, Ga, In 및 Au 중 적어도 하나로 이루어질 수 있으나, 이에 제한되는 것은 아니다.In addition, the conductive layer deposited on the pillar may refer to a layer made of a conductive material. The conductive layer may be formed of at least one of Ni, Zn, Pd, Ag, Cd, Pt, Ga, In, and Au, but is not limited thereto.
상기 제2전극(212b)은 기판(211) 상에서 상기 제1전극(212a)에 대향하는 대 전극(counter electrode)을 의미할 수 있다. 따라서, 표적 물질과의 반응에 의해 상기 제1전극(212a)에서 산화 반응이 일어난다면, 상기 제2전극(212b)에서는 환원 반응이 일어날 수 있다. 상기 제2전극(212b) 상에는 도전층이 증착된 상술한 필라가 배치될 수 있다.The second electrode 212b may refer to a counter electrode facing the first electrode 212a on the substrate 211 . Accordingly, if an oxidation reaction occurs in the first electrode 212a by reaction with a target material, a reduction reaction may occur in the second electrode 212b. The above-described pillars having a conductive layer deposited thereon may be disposed on the second electrode 212b.
상기 제3전극(212c)은 표적 물질과의 접촉 시에도 전위가 안정적으로 유지되는 기준 전극(reference electrode)을 의미할 수 있다. 상기 제3전극(212c) 상에는 표적 물질과의 접촉시에도 일정한 전위를 유지할 수 있는 반응층이 마련될 수 있다. The third electrode 212c may refer to a reference electrode in which a potential is stably maintained even when in contact with a target material. A reaction layer capable of maintaining a constant potential even when in contact with a target material may be provided on the third electrode 212c.
반응층은 Ag/AgCl, Ag, Hg2SO4, Ag/Ag+, Hg/Hg2SO4, RE-6H, Hg/HgO, Hg/Hg2Cl2, Ag/Ag2SO4, Cu/CuSO4, KCl 포화된 칼로멜 반전지(SCE) 및 염다리 백금의 전위차가 미리 알려진 물질로 이루어질 수 있다.The reaction layer is Ag/AgCl, Ag, Hg2SO4, Ag/Ag+, Hg/Hg2SO4, RE-6H, Hg/HgO, Hg/Hg2Cl2, Ag/Ag2SO4, Cu/CuSO4, KCl saturated calomel half cell (SCE) and salt bridge The potential difference of platinum may be made of a known material in advance.
도 3을 참조하면, 상기 밀리웰 또는 마이크로웰 구조(220)는 상기 전극(212) 상부에 구비되는 것으로, 복수 개의 홈으로 이루어진 것이다. 상기 밀리웰 또는 마이크로웰 구조(220)는 상기 센싱모듈(200)에 상기 분석 시료가 삽입되는 입구부에 구비될 수 있는 것으로, 상기 밀리웰 또는 마이크로웰 구조(220)를 사용함에 따라 분해능을 향상시킬 수 있는 장점이 있다. Referring to FIG. 3 , the milliwell or microwell structure 220 is provided on the electrode 212 and includes a plurality of grooves. The milliwell or microwell structure 220 may be provided at an inlet into which the analysis sample is inserted into the sensing module 200 , and the resolution is improved by using the milliwell or microwell structure 220 . There are advantages to doing it.
구체적으로, 상기 밀리웰 또는 마이크로웰 구조(220)는 작업 전극(working electrode) 상부에 구비될 수 있는 것으로, 상기 밀리웰 또는 마이크로웰 구조(220)는 작업 전극으로 이루어진 상기 제1전극(212a) 상부에 구비될 수 있다. Specifically, the milliwell or microwell structure 220 may be provided above a working electrode, and the milliwell or microwell structure 220 includes the first electrode 212a formed of a working electrode. It may be provided on the top.
본 발명의 실시예에 따른 상기 센싱모듈(200)은, 접속패드(230), 접속포트(240), 하우징(250), 본딩 와이어(260)를 더 포함할 수 있다. The sensing module 200 according to an embodiment of the present invention may further include a connection pad 230 , a connection port 240 , a housing 250 , and a bonding wire 260 .
상기 접속패드(230)는 단수 또는 복수 개가 구비될 수 있는 것으로, 상기 본딩 와이어(260)를 통해 상기 센서(210)와 전기적으로 연결될 수 있는 것이다. 도 4 및 도 6을 참조하면, A single or a plurality of connection pads 230 may be provided, and may be electrically connected to the sensor 210 through the bonding wire 260 . 4 and 6,
상기 센서(210)의 저면에는 복수 개의 상기 접속패드(230)와 상기 센서(210)에 구비된 복수 개의 전극(212)을 서로 전기적으로 연결하는 연결패드(213)가 더 마련될 수도 있다. A connection pad 213 for electrically connecting the plurality of connection pads 230 and the plurality of electrodes 212 provided in the sensor 210 to each other may be further provided on the bottom surface of the sensor 210 .
상기 센서(210)의 저면에 부착된 상기 연결패드(213)의 일면은 상기 센서(210)에 구비된 복수 개의 전극(212)과 전기적으로 연결되는 것으로, 상기 연결패드(213)의 타면은 상기 본딩 와이어(260)를 통하여 복수의 상기 접속패드(230)와 전기적으로 연결될 수 있다. 구체적으로, 복수 개의 상기 접속패드(230) 및 상기 연결패드(213)는 동박형태로 구비되고, 복수 개의 상기 접속패드(230)의 표면에는 금박이 더 코팅될 수 있다. One surface of the connection pad 213 attached to the bottom surface of the sensor 210 is electrically connected to the plurality of electrodes 212 provided in the sensor 210 , and the other surface of the connection pad 213 is the It may be electrically connected to the plurality of connection pads 230 through the bonding wire 260 . Specifically, the plurality of connection pads 230 and the connection pads 213 may be provided in a copper foil shape, and a surface of the plurality of connection pads 230 may be further coated with gold foil.
또한, 상기 연결패드(213)와 상기 센서(210)는 전도성 물질(미도시)을 통해 서로 전기적으로 연결될 수 있으며, 전도성 물질은 양면 접착 카본 테이프 등이 사용될 수 있다. 다만, 전도성 물질은 이에 한정되는 것은 아니며, 상기 연결패드(213)와 상기 센서(210)를 전기적으로 연결할 수 있다면 다양한 물질이 사용될 수 있음은 물론이다. In addition, the connection pad 213 and the sensor 210 may be electrically connected to each other through a conductive material (not shown), and a double-sided adhesive carbon tape may be used as the conductive material. However, the conductive material is not limited thereto, and various materials may be used as long as the connection pad 213 and the sensor 210 can be electrically connected.
상기 본딩 와이어(260)는 금(Au)으로 구성되며, 상기 본딩 와이어(260)의 일단은 상기 센서(210)에 부착된 상기 연결패드(213)에 본딩되어 상기 센서(210)에 구비된 복수 개의 상기 전극(212)과 전기적으로 연결되고, 상기 본딩 와이어(260)의 타단은 복수 개의 상기 접속패드(230)에 본딩되어 복수 개의 상기 접속패드(230)와 전기적으로 연결될 수 있다.The bonding wire 260 is made of gold (Au), and one end of the bonding wire 260 is bonded to the connection pad 213 attached to the sensor 210 , and is provided in the sensor 210 . The electrodes 212 may be electrically connected, and the other end of the bonding wire 260 may be bonded to the plurality of connection pads 230 to be electrically connected to the plurality of connection pads 230 .
도 3 및 도 4를 참조하면, 상기 접속포트(240)는 복수 개의 상기 접속패드(230)와 전기적으로 연결되고, 상기 접속모듈(100)에 결합되어 상기 접속모듈(100)과 전기적으로 연결될 수 있는 것이다. 3 and 4 , the connection port 240 may be electrically connected to the plurality of connection pads 230 , and may be coupled to the connection module 100 to be electrically connected to the connection module 100 . there will be
상기 접속포트(240)는 전선(261)을 통하여 복수 개의 상기 접속패드(230)와 전기적으로 연결될 수 있으며, 상기 접속모듈(100)에 결합 시 복수 개의 상기 접속패드(230)를 통해 상기 센서(210)에 전원을 공급하고, 상기 센서(210)에서 발생된 전기신호를 상기 접속모듈(100)로 전송할 수 있다. 상기 접속포트(240)는 상기 접속모듈(100)에 구비된 상기 제2접속단자(120)에 대응되는 형태로 구비될 수 있다. The connection port 240 may be electrically connected to the plurality of connection pads 230 through an electric wire 261 , and when coupled to the connection module 100 , the sensor ( Power may be supplied to the 210 , and an electrical signal generated from the sensor 210 may be transmitted to the connection module 100 . The connection port 240 may be provided in a shape corresponding to the second connection terminal 120 provided in the connection module 100 .
상기 하우징(250)은 상기 센서(210), 복수 개의 상기 접속패드(230), 상기 접속포트(240)가 설치되는 것으로, 상기 하우징(250)은 상기 센서(210), 복수 개의 상기 접속패드(230), 상기 접속포트(240)의 둘레를 감싸 상기 센서(210), 복수 개의 상기 접속패드(230), 상기 접속포트(240)를 외부로부터 보호하도록 구성될 수 있다. The housing 250 includes the sensor 210 , the plurality of connection pads 230 , and the connection port 240 installed therein. The housing 250 includes the sensor 210 and the plurality of connection pads ( 230), may be configured to surround the circumference of the connection port 240 to protect the sensor 210, the plurality of connection pads 230, and the connection port 240 from the outside.
상기 하우징(250)은 외부에서 제공된 분석 시료를 상기 센서(210)의 상기 전극(212)으로 안내하는 채널(253)이 구비된 제1부재(251)와 상기 제1부재(251)에 대향 배치되어 상기 제1부재(251)에 결합되고, 상기 센서(210), 복수 개의 상기 접속패드(230), 상기 접속포트(240)가 안착되는 제2부재(252)를 포함한다. The housing 250 is disposed opposite to the first member 251 and the first member 251 having a channel 253 for guiding the analyte sample provided from the outside to the electrode 212 of the sensor 210 . and is coupled to the first member 251 and includes a second member 252 on which the sensor 210 , the plurality of connection pads 230 , and the connection port 240 are seated.
상기 채널(253)은 상기 제1부재(251)를 관통하여 형성된 구멍으로, 상기 채널(253)은 외부에서 제공된 분석 시료를 상기 센서(210)의 상기 전극(212)으로 안내하기 위한 경사면(254)을 포함한다. 상기 경사면(254)은 상기 채널(253)의 둘레를 따라 구비될 수 있는 것으로, 외부에서 제공된 분석 시료는 상기 경사면(254)을 따라 이동하여 안정적으로 상기 센서(210)의 상기 전극(212)로 도달할 수 있게 된다. The channel 253 is a hole formed through the first member 251 , and the channel 253 is an inclined surface 254 for guiding an analysis sample provided from the outside to the electrode 212 of the sensor 210 . ) is included. The inclined surface 254 may be provided along the circumference of the channel 253 , and the analysis sample provided from the outside moves along the inclined surface 254 stably to the electrode 212 of the sensor 210 . be able to reach
여기서, 도 5를 참조하면, 상기 채널(253)에는 상기 밀리웰 또는 마이크로웰 구조(220)가 구비될 수 있다. 상기 밀리웰 또는 마이크로웰 구조(220)는 상기 제1전극(212a)-작업 전극(working electrode)의 상부에 구비될 수 있는 것으로, 상기 채널(253)은 상기 제1전극(212a)-작업 전극(working electrode)의 상부에 구비될 수 있다. Here, referring to FIG. 5 , the milliwell or microwell structure 220 may be provided in the channel 253 . The milliwell or microwell structure 220 may be provided on the first electrode 212a-working electrode, and the channel 253 is the first electrode 212a-working electrode. (working electrode) may be provided on top.
따라서, 상기 밀리웰 또는 마이크로웰 구조(220)는 상기 채널(253)에 구비되는 것이 바람직하며, 상기 밀리웰 또는 마이크로웰 구조(220)는 상기 채널(253)에 결합되면서 지지될 수 있게 된다. 상기 밀리웰 또는 마이크로웰 구조(220)를 상기 채널(253)에 구비함에 따라, 분석된 시료를 상기 채널(253)을 통해 상기 전극(212)으로 이동시킬 때 분해능을 향상시킬 수 있게 된다. Accordingly, the milliwell or microwell structure 220 is preferably provided in the channel 253 , and the milliwell or microwell structure 220 can be supported while being coupled to the channel 253 . As the milliwell or microwell structure 220 is provided in the channel 253 , it is possible to improve resolution when the analyzed sample is moved to the electrode 212 through the channel 253 .
상기 제1부재(251)는 상기 제2부재(252)에 결합되어, 상기 제2부재(252)에 배치된 상기 센서(210) 및 상기 접속포트(240)를 덮어 이들을 외부로부터 보호할 수 있다. 또한, 상기 제1부재(251)는 상기 센서(210) 및 상기 접속포트(240)를 소정의 힘으로 가압하여 이들을 견고하게 지지할 수도 있다. 또한, 상기 제1부재(251)는 상기 제2부재(252)와의 결합을 통하여 상기 채널(253)을 통해 상기 센서(210)로 제공된 분석 시료가 상기 하우징(250)의 외부로 누수되는 것을 방지할 수 있다. The first member 251 may be coupled to the second member 252 to cover the sensor 210 and the connection port 240 disposed on the second member 252 to protect them from the outside. . Also, the first member 251 may press the sensor 210 and the connection port 240 with a predetermined force to firmly support them. In addition, the first member 251 prevents the analysis sample provided to the sensor 210 through the channel 253 from leaking to the outside of the housing 250 through coupling with the second member 252 . can do.
도 4 및 도 6을 참조하면, 상기 제1부재(251)와 상기 제2부재(252) 사이에는, 상기 센서(210), 복수 개의 상기 접속패드(230), 상기 접속포트(240)를 수용하고, 본딩 와이어(260)를 보호하는 수용공간(250a)이 구비될 수 있다. 상기 수용공간(250a)은 상기 제1부재(251)와 상기 제2부재(252)의 내측에 각각 음각 패턴의 형태로 구비될 수 있다. 4 and 6 , the sensor 210 , the plurality of connection pads 230 , and the connection port 240 are accommodated between the first member 251 and the second member 252 . and an accommodation space 250a for protecting the bonding wire 260 may be provided. The accommodating space 250a may be provided in the form of an intaglio pattern inside the first member 251 and the second member 252, respectively.
도 5를 참조하면, 상기 제2부재(252)에는, 상기 제1부재(251)와 결합되고, 상기 제1부재(251)를 지지하는 적어도 하나의 결합돌기(252a)가 마련되고, 상기 제1부재(251)에는 상기 결합돌기(252a)에 대응되는 적어도 하나의 결합홈(251a)이 구비될 수 있다. Referring to FIG. 5 , at least one coupling protrusion 252a coupled to the first member 251 and supporting the first member 251 is provided on the second member 252 , One member 251 may be provided with at least one coupling groove 251a corresponding to the coupling protrusion 252a.
상기 결합돌기(252a)와 상기 결합홈(251a)을 결합하면, 상기 채널(253)의 중심과 상기 센서(210)의 중심이 자연스럽게 정해진 위치에 정렬될 수 있다. 상기 결합홈(251a)의 내측에는, 상기 결합돌기(252a)가 상기 결합홈(251a)에 삽입될 경우 상기 결합돌기(252a)와 상기 결합홈(251a) 사이를 기밀하는 고무 또는 실리콘 재질의 실러가 더 구비될 수 있다. When the coupling protrusion 252a and the coupling groove 251a are coupled, the center of the channel 253 and the center of the sensor 210 may be naturally aligned at a predetermined position. On the inside of the coupling groove 251a, when the coupling protrusion 252a is inserted into the coupling groove 251a, a sealer made of a rubber or silicone material that seals the space between the coupling protrusion 252a and the coupling groove 251a. may be further provided.
도 5를 참조하면, 상기 제2부재(252)에는 상기 센서(210)가 안착되어 지지되는 안착홈(252b)과 상기 센서(210)의 외측으로 흘러내린 분석 시료가 저장되는 수거홈(252c)이 더 구비될 수 있다. 상기 센서(210)와 상기 수거홈(252c) 사이에는 상기 센서(210)의 외측으로 흐르는 분석 시료를 상기 수거홈(252c) 측으로 안내하는 시료 안내면(252d)이 더 마련될 수 있으며, 상기 시료 안내면(252d)은 경사진 형태로 이루어질 수 있다. Referring to FIG. 5 , the second member 252 has a seating groove 252b in which the sensor 210 is seated and supported, and a collection groove 252c in which an analysis sample flowing down from the sensor 210 is stored. This may be further provided. A sample guide surface 252d for guiding the analysis sample flowing out of the sensor 210 toward the collection groove 252c may be further provided between the sensor 210 and the collection groove 252c, and the sample guide surface (252d) may be formed in an inclined shape.
도 3 및 도 4를 참조하면, 상기 하우징(250)은 분석 시료의 흐름 상태, 분석 시료의 누수 여부, 상기 채널(253)과 상기 센서(210)의 정렬상태, 상기 본딩 와이어(260)를 통한 상기 센서(210)와 복수 개의 상기 접속패드(230)의 연결상태 및 복수 개의 상기 접속패드(230)와 상기 접속포트(240)의 전기적 연결상태 등을 외부에서 관찰하기 용이하도록, 투명한 재질로 마련될 수 있다. Referring to FIGS. 3 and 4 , the housing 250 includes a flow state of an analyte sample, leakage of an analyte sample, an alignment state of the channel 253 and the sensor 210 , and the bonding wire 260 through The sensor 210 and the plurality of connection pads 230 are connected to each other and the electrical connection between the plurality of connection pads 230 and the connection port 240 can be easily observed from the outside, and it is made of a transparent material. can be
가령, 상기 하우징(250)은 폴리메칠메타크릴레이트, 폴리카보네이트, 시클릭올레핀코폴리머(Cyclic olefine copolymer), 폴리에틸렌술폰(Polyethylene sulfone) 및 폴리스티렌 중 적어도 하나의 재질로 마련되거나, 이들 중 적어도 둘 이상이 조합된 재질로 마련될 수 있다. 그러나 상기 하우징(250)의 재질은 반드시 이에 한정되는 것은 아니며, 실리콘 기반의 유기폴리머인 폴리디메틸실록산 재질로 구성될 수도 있다.For example, the housing 250 is made of at least one of polymethyl methacrylate, polycarbonate, cyclic olefin copolymer, polyethylene sulfone, and polystyrene, or at least two or more of them. It may be provided with these combined materials. However, the material of the housing 250 is not necessarily limited thereto, and may be made of a polydimethylsiloxane material, which is a silicone-based organic polymer.
본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서는 다음과 같이 변형되어 사용될 수도 있다. The biosensor using the milliwell or microwell structure according to an embodiment of the present invention may be modified and used as follows.
도 7을 참조하면, 본 발명의 실시예에 따른 바이오 센서의 상기 센싱모듈(200)은 복수 개가 구비될 수 있으며, 복수 개의 상기 센싱모듈(200)은 상기 접속모듈(100)에 탈부착 가능하게 결합될 수 있다. 즉, 상기 접속모듈(100)은 복수 개의 상기 센싱모듈(200)과 전기적으로 연결될 수 있다. Referring to FIG. 7 , a plurality of sensing modules 200 of the biosensor according to an embodiment of the present invention may be provided, and the plurality of sensing modules 200 are detachably coupled to the connection module 100 . can be That is, the connection module 100 may be electrically connected to the plurality of sensing modules 200 .
이를 위해, 상기 접속모듈(100)은 상기 외부장치(20)와 결합 가능한 상기 제1접속단자(110)와 상기 센싱모듈(200)에 결합 가능한 제2접속단자(120)를 포함하면서, 상기 제2접속단자(120)는 복수 개가 구비될 수 있다. 복수 개의 상기 센싱모듈(200)은 복수 개의 상기 제2접속단자(120)에 선택적으로 결합될 수 있다. To this end, the connection module 100 includes the first connection terminal 110 that can be coupled to the external device 20 and a second connection terminal 120 that can be coupled to the sensing module 200, A plurality of 2 connection terminals 120 may be provided. The plurality of sensing modules 200 may be selectively coupled to the plurality of second connection terminals 120 .
이와 같이 상기 센싱모듈(200)을 복수 개 구비하면서 상기 제2접속단자(120)를 복수 개 구비하면, 서로 다른 표적 물질을 감지하여 다수의 검사를 한 번에 수행할 수 있는 장점이 있다. In this way, when a plurality of the sensing modules 200 are provided and a plurality of the second connection terminals 120 are provided, there is an advantage in that different target materials can be detected and a plurality of tests can be performed at once.
여기서, 복수 개의 상기 센싱모듈(200)이 복수 개의 상기 제2접속단자(120)에 모두 연결되어 전기적 신호를 발생하는 경우, 상기 컨트롤러(130)는 복수 개의 상기 제2접속단자(120)를 통해 전기적 신호가 전달된 순서를 기억한 뒤, 순차적으로 해당 제2접속단자(120)에 연결된 상기 센싱모듈(200)의 전기적 신호를 수신하여 상기 제1접속단자(110)로 전송할 수 있다. Here, when the plurality of sensing modules 200 are all connected to the plurality of second connection terminals 120 to generate electrical signals, the controller 130 is configured to operate through the plurality of second connection terminals 120 . After storing the order in which the electrical signals are transmitted, the electrical signals of the sensing module 200 connected to the corresponding second connection terminal 120 may be sequentially received and transmitted to the first connection terminal 110 .
상기 접속모듈(100)은 복수 개의 상기 제2접속단자(120)와 상기 컨트롤러(130) 사이에서 복수 개의 상기 제2접속단자(120)와 상기 컨트롤러(130)의 접속을 제어하는 전원부(150)를 더 포함할 수 있다. The connection module 100 is a power supply unit 150 for controlling the connection between the plurality of second connection terminals 120 and the controller 130 between the plurality of second connection terminals 120 and the controller 130 . may further include.
상기 전원부(150)는 복수 개가 구비될 수 있으며, 복수 개의 상기 제2접속단자(120)와 개별적으로 연결될 수 있다. 또한, 상기 전원부(150)는 각각의 상기 컨트롤러(130)와 연결될 수 있다. A plurality of the power supply unit 150 may be provided, and may be individually connected to the plurality of second connection terminals 120 . Also, the power supply unit 150 may be connected to each of the controllers 130 .
상기 전원부(150)는 사용자의 조작을 통해 ON/OFF 제어되도록 구성될 수 있는 것으로, 사용자의 조작을 통하여 상기 전원부(150)가 ON 상태로 제어된 경우에만, 상기 제2접속단자(120)에 연결된 상기 센싱모듈(200)의 전기적 신호가 상기 컨트롤러(130)로 전송될 수 있다. The power supply unit 150 may be configured to be ON/OFF controlled through a user's operation. Only when the power supply unit 150 is controlled to an ON state through a user's operation, the power supply unit 150 is connected to the second connection terminal 120 . An electrical signal of the connected sensing module 200 may be transmitted to the controller 130 .
도 7 및 도 8을 참조하면, 상기 접속모듈(100)은 상기 제1접속단자(110)의 둘레에 설치되면서 상기 제1접속단자(110)가 미리 설정된 각도만큼 회전 가능하게 하는 각도 조절부(160)를 더 포함할 수 있다. 7 and 8, the connection module 100 is installed on the periphery of the first connection terminal 110, the angle adjusting unit ( 160) may be further included.
상기 각도 조절부(160)는 상기 제1접속단자(110)에 결합되어 상기 제1접속단자(110)와 함께 회전하도록 구성되는 회전부재(161)와 상기 본체(140)에 설치되고 내측에 상기 회전부재(161)가 수용되어 상기 회전부재(161)의 회전을 가이드하는 안내부재(162)를 포함할 수 있다. The angle adjusting unit 160 is coupled to the first connection terminal 110 and is installed on the main body 140 and the rotating member 161 configured to rotate together with the first connection terminal 110 and is provided inside the The rotating member 161 may be accommodated and may include a guide member 162 for guiding the rotation of the rotating member 161 .
상기 회전부재(161)의 외주면에는 서로 이격 배치된 복수 개의 돌기(161a)가 마련되고, 안내부재(162)의 내주면에는 복수 개의 상기 돌기(161a)에 대응되는 복수 개의 홈(162a)이 마련될 수 있다. 따라서, 사용자는 상기 제1접속단자(110)가 상기 외부장치(20)에 결합된 상태에서 상기 본체(140)를 회전시켜 복수 개의 상기 제2접속단자(120)를 소정의 위치로 배치시킬 수 있다.A plurality of projections 161a spaced apart from each other are provided on the outer peripheral surface of the rotating member 161, and a plurality of grooves 162a corresponding to the plurality of projections 161a are provided on the inner peripheral surface of the guide member 162. can Accordingly, the user can arrange the plurality of second connection terminals 120 in predetermined positions by rotating the main body 140 in a state in which the first connection terminal 110 is coupled to the external device 20 . have.
도 9를 참조하면, 본 발명의 실시예에 따르면, 상기 접속모듈(100)과 상기 센싱모듈(200)은 자성을 통해 서로 탈부착 가능하게 결합될 수 있으며, 면접촉을 통해 서로 전기적으로 연결될 수 있다. Referring to FIG. 9 , according to an embodiment of the present invention, the connection module 100 and the sensing module 200 may be detachably coupled to each other through magnetism, and may be electrically connected to each other through surface contact. .
이를 위해, 상기 접속모듈(100)에는 제1결합부재(170)가 구비되며, 상기 센싱모듈(200)에는 제1결합부재(170)와 결합 가능한 제2결합부재(270)가 구비될 수 있다. To this end, the connection module 100 may include a first coupling member 170 , and the sensing module 200 may include a second coupling member 270 capable of being coupled to the first coupling member 170 . .
도 10을 참조하면, 상기 제1결합부재(170)는 상기 접속모듈(100)의 일 단부로부터 돌출되는 결합돌기(171)와 상기 결합돌기(171)의 단부에 수용된 제1자성체(172)를 포함할 수 있다. Referring to FIG. 10 , the first coupling member 170 includes a coupling protrusion 171 protruding from one end of the connection module 100 and a first magnetic body 172 accommodated at an end of the coupling protrusion 171 . may include
상기 결합돌기(171)는 돌출방향을 따라 외경의 크기가 점차 감소하는 웨지(wedge) 구조로 형성될 수 있다. 이를 통해 상기 접속모듈(100)과 상기 센싱모듈(200)의 결합 시, 상기 결합돌기(171)의 경사진 외주면이 상기 센싱모듈(200)에 구비된 상기 제2결합부재(270)의 결합홈(271)의 내주면에 안내되어 용이하게 결합될 수 있다. The coupling protrusion 171 may be formed in a wedge structure in which the size of the outer diameter gradually decreases along the protrusion direction. Through this, when the connection module 100 and the sensing module 200 are coupled, an inclined outer circumferential surface of the coupling protrusion 171 is a coupling groove of the second coupling member 270 provided in the sensing module 200 . Guided to the inner peripheral surface of the (271) can be easily coupled.
상기 제2결합부재(270)는 상기 접속모듈(100)의 일 단부와 마주하는 상기 센싱모듈(200)의 타 단부에 오목하게 형성되는 상기 결합홈(271)과 상기 결합홈(271)의 내측에 수용되어 상기 제1자성체(172)와 자력을 통해 결합 가능한 제2자성체(272)를 포함할 수 있다. The second coupling member 270 is formed concavely on the other end of the sensing module 200 facing one end of the connection module 100 , and the coupling groove 271 is formed inside the coupling groove 271 . It is accommodated in the first magnetic body 172 and may include a second magnetic body 272 capable of being coupled through a magnetic force.
상기 접속모듈(100)과 상기 센싱모듈(200)은 상기 결합돌기(171)와 상기 결합홈(271)의 구조적인 결합을 통하여 1차적으로 결합되고, 상기 제1자성체(172)와 상기 제2자성체(272)의 자성을 이용한 결합을 통해 2차적으로 결합됨에 따라 서로 안정적으로 고정될 수 있다. The connection module 100 and the sensing module 200 are primarily coupled through the structural coupling of the coupling protrusion 171 and the coupling groove 271, and the first magnetic body 172 and the second As the magnetic body 272 is secondarily coupled through coupling using the magnetism, they may be stably fixed to each other.
도 9를 참조하면, 상기 센싱모듈(200)에는 상기 제2자성체(272)의 자기력이 상기 센싱모듈(200)의 다른 부분에 영향을 미치지 않도록, 상기 제2자성체(272)의 자기력을 차폐시키도록 구성되는 차폐 구조물(280)이 구비될 수 있다. 9, the sensing module 200 has the magnetic force of the second magnetic body 272 shielding the magnetic force of the second magnetic body 272 so as not to affect other parts of the sensing module 200. A shielding structure 280 configured to do so may be provided.
상기 차폐 구조물(280)은 상기 하우징(250)의 내측에 마련되고, 상기 제2결합부재(270)의 상기 결합홈(271)과 상기 제2자성체(272)의 둘레를 감싸는 형태로 구비될 수 있다. 상기 차폐 구조물(280)은 상기 하우징(250)과 동일한 재질로 형성되되, 자기력을 차폐시킬 수 있는 재질로 이루어질 수 있다. The shielding structure 280 may be provided inside the housing 250 and be provided in a shape surrounding the coupling groove 271 of the second coupling member 270 and the periphery of the second magnetic body 272 . have. The shielding structure 280 may be made of the same material as the housing 250 , and may be made of a material capable of shielding magnetic force.
본 발명의 실시예에 따르면, 상기 접속모듈(100)과 상기 센싱모듈(200)은 면접촉 결합을 통하여 서로 전기적으로 연결될 수 있다. 도 9를 참조하면, 상기 센싱모듈(200)의 상기 접속포트(240)와 연결되는 상기 접속모듈(100)의 상기 제2접속단자(120)는 면접촉을 통해 서로 전기적으로 연결 가능한 평판형의 단자 형태로 구비될 수 있다. According to an embodiment of the present invention, the connection module 100 and the sensing module 200 may be electrically connected to each other through surface contact coupling. Referring to FIG. 9 , the second connection terminal 120 of the connection module 100 connected to the connection port 240 of the sensing module 200 is a flat plate type that can be electrically connected to each other through surface contact. It may be provided in the form of a terminal.
상기 접속모듈(100)의 상기 제1결합부재(170)와 상기 센싱모듈(200)의 상기 제2결합부재(270)가 서로 결합되면, 상기 접속포트(240)와 상기 제2접속단자(120)는 면접촉되는 것만으로 서로 전기적으로 연결될 수 있다. When the first coupling member 170 of the connection module 100 and the second coupling member 270 of the sensing module 200 are coupled to each other, the connection port 240 and the second connection terminal 120 ) can be electrically connected to each other just by being interviewed.
이를 통해 상기 접속모듈(100)과 상기 센싱모듈(200)의 결합 및 분리가 더욱 용이해지며, 탈부착 식에도 상기 접속포트(240)와 상기 제2접속단자(120)가 파손되는 것을 방지할 수 있게 된다. This makes it easier to combine and separate the connection module 100 and the sensing module 200, and it is possible to prevent the connection port 240 and the second connection terminal 120 from being damaged even in a detachable type. there will be
본 발명의 실시예에 따른 상기 센싱모듈(200)의 단부에는, 훼손 유무를 통해 상기 센싱모듈(200)의 사용여부를 확인 가능한 식별필름이 부착될 수 있다. 상기 센싱모듈(200)의 단부에는 상기 접속모듈(100)의 단부에 마련된 웨지형상의 가압돌기(미도시)와 결합 가능한 수용홈(미도시)이 마련되고, 수용홈의 입구에는 상기 센싱모듈(200)의 단부에 부착되어 수용홈의 입구를 차단하도록 구성되는 박막형태의 상기 식별필름이 부착될 수 있다. An identification film may be attached to the end of the sensing module 200 according to an embodiment of the present invention to confirm whether or not the sensing module 200 is used through the presence or absence of damage. At the end of the sensing module 200, a receiving groove (not shown) capable of engaging with a wedge-shaped pressing protrusion (not shown) provided at the end of the connection module 100 is provided, and at the entrance of the receiving groove, the sensing module ( 200) may be attached to the identification film in the form of a thin film configured to block the entrance of the receiving groove.
상기 식별필름은 상기 센싱모듈(200)과 상기 접속모듈(100)의 결합 시 수용홈으로 삽입되는 상기 접속모듈(100)의 가압돌기에 의해 소정의 힘으로 가압되어 파손되도록 구성될 수 있다. 따라서, 사용자는 상기 센싱모듈(200)의 단부에 마련된 식별필름의 훼손유무를 확인하여 상기 센싱모듈(200)의 사용여부를 파악할 수 있다. 다만, 센싱모듈(200)의 사용여부를 확인 가능한 구성은 반드시 이에 한정되는 것은 아니며, 다양한 형태로 변경되어 적용될 수 있다.The identification film may be configured to be damaged by being pressed with a predetermined force by the pressing protrusion of the connection module 100 inserted into the receiving groove when the sensing module 200 and the connection module 100 are coupled. Accordingly, the user can determine whether the sensing module 200 is used by checking whether the identification film provided at the end of the sensing module 200 is damaged. However, the configuration capable of confirming whether the sensing module 200 is used is not necessarily limited thereto, and may be changed and applied in various forms.
도 11을 참조하면, 본 발명의 실시예에 따른 상기 센싱모듈(200)은 분석 시료로부터 표적물질을 감지하도록 구성되는 복수 개의 센싱부를 포함할 수 있다. 복수 개의 상기 센싱부는 상기 접속포트(240)와 전기적으로 연결되는 것으로, 상기 하우징(250) 내부에 설치될 수 있다. Referring to FIG. 11 , the sensing module 200 according to an embodiment of the present invention may include a plurality of sensing units configured to detect a target material from an analysis sample. The plurality of sensing units are electrically connected to the connection port 240 , and may be installed inside the housing 250 .
복수 개의 상기 센싱부 각각에는 상기 센서(210)를 포함하며, 복수 개의 상기 센싱부는 격벽(214)을 통해 서로 분리되되, 상기 접속모듈(100)과 전기적으로 결합 가능한 상기 접속포트(240)를 통해 서로 전기적으로 연결될 수 있는 것이다. Each of the plurality of sensing units includes the sensor 210 , and the plurality of sensing units are separated from each other through a partition wall 214 , through the connection port 240 electrically coupleable to the connection module 100 . They can be electrically connected to each other.
복수 개의 상기 센싱부는 제1센싱부(210a)와 제2센싱부(210b)를 포함할 수 있다. 상기 제1센싱부(210a)와 상기 제2센싱부(210b)는 상기 하우징(250) 내에 구비된 상기 격벽(214)을 통하여 서로 독립된 공간에 배치될 수 있다. The plurality of sensing units may include a first sensing unit 210a and a second sensing unit 210b. The first sensing unit 210a and the second sensing unit 210b may be disposed in spaces independent from each other through the partition wall 214 provided in the housing 250 .
상기 제1센싱부(210a)와 상기 제2센싱부(210b)는 각각 접촉된 분석 시료로부터 표적 물질을 감지하고, 표적 물질과 반응하여 전기적인 신호를 발생시키는 상기 센서(210) 및 상기 본딩 와이어(260)를 통하여 상기 센서(210)와 전기적으로 연결되고, 상기 전선(261)을 통하여 상기 접속포트(240)와 전기적으로 연결되는 상기 접속패드(230)를 포함할 수 있다.The first sensing unit 210a and the second sensing unit 210b each sense a target material from the contacted analyte, and the sensor 210 and the bonding wire that react with the target material to generate an electrical signal The connection pad 230 may be electrically connected to the sensor 210 through 260 and electrically connected to the connection port 240 through the electric wire 261 .
또한, 상기 제1센싱부(210a)와 상기 제2센싱부(210b)는 상기 채널(253)이 각각 구비될 수 있으며, 각각의 상기 채널(253)에는 상기 밀리웰 또는 마이크로웰 구조(220)가 구비될 수 있다. In addition, the first sensing unit 210a and the second sensing unit 210b may each include the channel 253 , and each of the channels 253 has the milliwell or microwell structure 220 . may be provided.
또한, 상기 제1센싱부(210a)와 상기 제2센싱부(210b) 각각에는 상기 접속패드(230)와 상기 접속포트(240) 사이에서 상기 접속패드(230)와 상기 접속포트(240)의 접속을 제어하는 전원부(290)가 더 구비될 수 있다. In addition, in each of the first sensing unit 210a and the second sensing unit 210b, between the connection pad 230 and the connection port 240 , the connection pad 230 and the connection port 240 are provided. A power supply unit 290 for controlling the connection may be further provided.
상기 전원부(290)는 상기 접속패드(230)와 상기 접속포트(240)에 전기적으로 연결되고, 사용자의 조작을 통해 ON/OFF 제어되도록 구성될 수 있다. 따라서, 사용자의 조작을 통하여 상기 전원부(290)가 ON 상태로 제어된 경우에만, 상기 접속패드(230)와 연결된 상기 센서(210)의 전기적 신호가 상기 접속포트(240)로 전송될 수 있다. The power supply unit 290 is electrically connected to the connection pad 230 and the connection port 240 , and may be configured to be ON/OFF controlled through a user's manipulation. Accordingly, only when the power supply unit 290 is controlled to be in the ON state through a user's manipulation, the electrical signal of the sensor 210 connected to the connection pad 230 may be transmitted to the connection port 240 .
본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서는 상기 제1센싱부(210a)와 상기 제2센싱부(210b)를 포함하는 것으로 설명하였으나, 이에 한정되는 것은 아니며, 2개 이상의 센싱부가 구비될 수도 있다. The biosensor using the milliwell or microwell structure according to the embodiment of the present invention has been described as including the first sensing unit 210a and the second sensing unit 210b, but is not limited thereto, and 2 More than one sensing unit may be provided.
상술한 본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서는 다음과 같은 효과가 있다. The biosensor using the milliwell or microwell structure according to the embodiment of the present invention described above has the following effects.
본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서(10)는 USB 또는 핀 형태로 마련된 접속단자를 통해 외부장치(20)와 전기적으로 연결되도록 구성되므로, 다양한 장치에 적용 가능하여, 비용을 절감할 수 있음은 물론, 실생활에서도 쉽게 사용 가능하여 편의성 및 제품의 활용성이 증대될 수 있다.The biosensor 10 using a milliwell or microwell structure according to an embodiment of the present invention is configured to be electrically connected to the external device 20 through a connection terminal provided in the form of a USB or pin, so it can be applied to various devices. Accordingly, it is possible to reduce costs, as well as to be easily used in real life, so that convenience and usability of the product can be increased.
또한, 본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서(10)는 외부장치(20)와 전기적으로 연결되는 접속모듈(100)과, 접속모듈(100)에 탈착 가능한 센싱모듈(200)로 구성되므로, 센싱모듈(200)의 교체를 통해 다양한 종류의 검사를 수행 가능하고, 접속모듈(100)이 외부장치(20)에 결합된 상태에서 센싱모듈(200)만 교체 가능하여 연속적으로 신속한 검사를 수행할 수 있다.In addition, the biosensor 10 using a milliwell or microwell structure according to an embodiment of the present invention includes a connection module 100 electrically connected to an external device 20 , and sensing detachable from the connection module 100 . Since it is composed of the module 200, various types of tests can be performed by replacing the sensing module 200, and only the sensing module 200 can be replaced while the connection module 100 is coupled to the external device 20 Thus, it is possible to perform continuous and rapid inspection.
또한, 본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서(10)는 접속모듈(100)을 매개로 센싱모듈(200)과 외부장치(20)가 서로 전기적으로 연결되도록 구성되므로, 바이오 센서(10)를 외부장치(20)에 결합하거나 분리할 경우에도 센싱부위가 외부장치(20)에 의해 손상되는 것이 방지된다.In addition, the biosensor 10 using a milliwell or microwell structure according to an embodiment of the present invention is configured such that the sensing module 200 and the external device 20 are electrically connected to each other via the connection module 100 . Therefore, even when the biosensor 10 is coupled to or separated from the external device 20 , the sensing portion is prevented from being damaged by the external device 20 .
또한, 본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서(10)에 적용되는 기판(211)에 마련되는 전극의 형상이 개선됨으로써, 종래 대비 더 많은 수량의 센서(210)의 제작이 가능하게 되어, 제조비용이 절감될 수 있다. In addition, since the shape of the electrode provided on the substrate 211 applied to the biosensor 10 using the milliwell or microwell structure according to the embodiment of the present invention is improved, a larger number of sensors 210 than in the prior art It is possible to manufacture the , so that the manufacturing cost can be reduced.
구체적으로, 기판(211)에 마련되는 전극의 형상이 개선됨으로써, 센서(210)를 2 x 2 mm 크기로 제작 가능하게 되고. 따라서 기존에 8인치 웨이퍼를 이용하여 제작 가능한 센서(210)의 수량이 적게는 1000개에서 많게는 2만개까지 크게 증가하였다. 센서(210)를 제외한 타 부분은 제조 단가가 저렴한 PCB 공정을 이용하여 제조비용을 더욱 절감할 수 있다.Specifically, as the shape of the electrode provided on the substrate 211 is improved, the sensor 210 can be manufactured in a size of 2 x 2 mm. Therefore, the number of sensors 210 that can be manufactured using an existing 8-inch wafer has greatly increased from a low of 1000 to a high of 20,000. Except for the sensor 210, the manufacturing cost can be further reduced by using a low-cost PCB process.
이와 함께, 본 발명의 실시예에 따른 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서(10)는 밀리웰 또는 마이크로웰 구조(220)를 통해 분석 시료를 삽입함에 따라 분해능을 향상시킬 수 있는 장점이 있다. In addition, the biosensor 10 using a milliwell or microwell structure according to an embodiment of the present invention has the advantage of improving resolution by inserting an analysis sample through the milliwell or microwell structure 220 . have.
이와 같이 본 발명은 도면에 도시된 실시예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 다른 실시예가 가능하다는 점을 이해할 것이다. 따라서 본 발명의 진정한 기술적 보호 범위는 첨부된 청구범위의 기술적 사상에 의하여 정해져야 할 것이다.As such, the present invention has been described with reference to the embodiment shown in the drawings, which is merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. . Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.
[부호의 설명][Explanation of code]
10...바이오 센서 20...외부장치10...biosensor 20...external device
100...접속모듈 110...제1접속단자100...connection module 110...first connection terminal
120...제2접속단자 130...컨트롤러120...Second connection terminal 130...Controller
140...본체 150...전원부140...Main unit 150...Power supply
160...각도 조절부 161...회전부대160...Angle adjustment unit 161...Rotating unit
162...안내부재 170...제1결합부재162...Guide member 170...First coupling member
171...결합돌기 172...제1자성체171...bonding protrusion 172...first magnetic body
200...센싱모듈 210...센서200...sensing module 210...sensor
211...기판 212...전극211...substrate 212...electrode
212a...제1전극 212b...제2전극212a...first electrode 212b...second electrode
212c...제3전극 213...연결패드212c...third electrode 213...connection pad
214...격벽 220...밀리웰 또는 마이크로웰 구조214...Bulkhead 220...Milliwell or microwell construction
230...접속패드 240...접속포트230...connection pad 240...connection port
250...하우징 250a...수용공간250...housing 250a...accommodating space
251...제1부재 251a...결합홈251... First member 251a... Combination groove
252...제2부재 252a...결합돌기 252...Second member 252a...Joining projection
252b...안착홈 252c...수거홈252b...Receiving groove 252c...Collection groove
252d...시료 안내면 253...채널252d...Sample guide plane 253...Channel
254...경사면 260...본딩 와이어 254...Slope 260...bonding wire
261...전선 270...제2결합부재261...Wire 270...Second coupling member
271...결합홈 272...제2자성체271...Coupling groove 272...Second magnetic body
280...차폐 구조물 290...전원부280...shield structure 290...power
Claims (10)
- 외부장치와 전기적으로 연결 가능한 접속모듈; a connection module electrically connectable to an external device;내부로 유입된 분석 시료로부터 표적 물질을 감지하고, 상기 감지된 표적 물질과 반응하여 발생된 전기적인 신호를 상기 접속모듈에 전송하도록 구성되는 센싱모듈;을 포함하고, A sensing module configured to detect a target material from the analysis sample introduced into the inside, and transmit an electrical signal generated by reacting with the sensed target material to the connection module;상기 센싱모듈에는, 상기 분석 시료로부터 상기 표적 물질을 감지하고, 상기 표적 물질과 반응하여 상기 전기적인 신호를 발생시키며 센서가 구비되며, The sensing module is provided with a sensor that detects the target material from the analyte sample, reacts with the target material to generate the electrical signal,상기 센서는, The sensor is기판;과 상기 기판의 일면에 마련되고, 상기 표적 물질과 반응하여 전기 화학적 신호를 발생시키는 복수개의 전극;과 상기 전극 상부에 구비되며, 복수 개의 홈으로 이루어진 밀리웰 또는 마이크로웰 구조;를 포함하며, a substrate; a plurality of electrodes provided on one surface of the substrate to react with the target material to generate an electrochemical signal; and a milliwell or microwell structure provided on the electrode and comprising a plurality of grooves; and ,상기 센싱모듈은 상기 접속모듈에 탈부착 가능한 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.The sensing module is a biosensor using a milliwell or microwell structure, characterized in that it is detachable from the connection module.
- 제1항에 있어서, According to claim 1,상기 전극은, The electrode is상기 표적 물질과 산화 반응하는 제1전극과,a first electrode that oxidizes with the target material;상기 표적 물질과 환원 반응하는 제2전극과,a second electrode for reducing reaction with the target material;상기 제1전극과 상기 제2전극 사이에 일정한 작동전압이 유지되도록 구성되는 제3전극을 포함하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.A biosensor using a milliwell or microwell structure including a third electrode configured to maintain a constant operating voltage between the first electrode and the second electrode.
- 제2항에 있어서, 3. The method of claim 2,상기 밀리웰 또는 마이크로웰 구조는, 상기 제1전극 상부에 구비되는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.The milliwell or microwell structure is a biosensor using a milliwell or microwell structure, characterized in that it is provided on the first electrode.
- 제1항에 있어서, According to claim 1,상기 접속모듈은,The connection module is상기 외부장치에 결합 가능한 제1접속단자와, a first connection terminal capable of being coupled to the external device;상기 센싱모듈에 결합 가능한 제2접속단자와, a second connection terminal capable of being coupled to the sensing module;상기 제1접속단자와 상기 제2접속단자 사이에서 신호의 전송을 제어하도록 구성되는 컨트롤러와, a controller configured to control transmission of a signal between the first connection terminal and the second connection terminal;상기 제1접속단자, 상기 제2접속단자 및 상기 컨트롤러가 설치되는 본체를 포함하는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.The biosensor using a milliwell or microwell structure, characterized in that it comprises a main body in which the first connection terminal, the second connection terminal, and the controller are installed.
- 제1항에 있어서, According to claim 1,상기 센싱모듈은,The sensing module,본딩 와이어를 통하여 상기 센서와 전기적으로 연결되는 접속패드와a connection pad electrically connected to the sensor through a bonding wire;상기 접속패드와 전기적으로 연결되고, 상기 접속모듈에 결합 가능한 접속포트와 a connection port electrically connected to the connection pad and capable of being coupled to the connection module;상기 센서, 상기 접속패드 및 상기 접속포트가 설치되는 하우징을 더 포함하는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.The biosensor using a milliwell or microwell structure, further comprising a housing in which the sensor, the connection pad, and the connection port are installed.
- 제5항에 있어서, 6. The method of claim 5,상기 하우징은, The housing is상기 분석 시료를 상기 센서의 상기 전극으로 안내하는 채널이 구비된 제1부재와, a first member provided with a channel for guiding the analysis sample to the electrode of the sensor;상기 제1부재에 대향 배치되어 제1부재에 결합되면서 상기 센서, 상기 접속패드, 상기 접속포트가 안착되는 제2부재를 포함하며, and a second member disposed opposite to the first member and coupled to the first member on which the sensor, the connection pad, and the connection port are seated;상기 밀리웰 또는 마이크로웰 구조는 상기 채널에 구비되는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.The milliwell or microwell structure is a biosensor using a milliwell or microwell structure, characterized in that provided in the channel.
- 제1항에 있어서, According to claim 1,상기 접속모듈과 상기 센싱모듈은, The connection module and the sensing module,자성을 통해 서로 탈부착 가능하게 결합되며, 면접촉을 통해 서로 전기적으로 연결되는 것은 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.A biosensor using a milliwell or microwell structure, characterized in that they are detachably coupled to each other through magnetism and electrically connected to each other through surface contact.
- 제1항에 있어서, According to claim 1,상기 센싱모듈은 복수 개가 구비되면서 상기 접속모듈에 탈부착 가능하게 결합되며, The sensing module is provided with a plurality and is detachably coupled to the connection module,상기 접속모듈은, The connection module is상기 외부장치와 결합 가능한 제1접속단자와, a first connection terminal capable of being coupled to the external device;상기 센싱모듈에 결합 가능한 제2접속단자를 포함하며, It includes a second connection terminal that can be coupled to the sensing module,상기 제1접속단자와 상기 제2접속단자 사이에서 신호의 전송을 제어하도록 구성되는 컨트롤러와, a controller configured to control transmission of a signal between the first connection terminal and the second connection terminal;상기 제1접속단자, 상기 제2접속단자 및 상기 컨트롤러가 설치되는 본체를 포함하며,and a main body in which the first connection terminal, the second connection terminal, and the controller are installed,상기 제2접속단자는 복수 개가 구비되는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.The second connection terminal is a biosensor using a milliwell or microwell structure, characterized in that provided with a plurality of terminals.
- 제8항에 있어서, 9. The method of claim 8,상기 접속모듈은, The connection module is상기 제2접속단자와 상기 컨트롤러 사이의 접속을 제어하는 전원부를 포함하는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.and a power supply unit for controlling a connection between the second connection terminal and the controller.
- 제1항에 있어서, According to claim 1,상기 센싱모듈에는, In the sensing module,상기 센서를 포함하는 센싱부가 복수 개 구비되며, A plurality of sensing units including the sensor are provided,복수 개의 상기 센싱부는 격벽을 통해 서로 분리되되, 상기 접속모듈과 전기적으로 결합 가능한 접속포트를 통해 서로 전기적으로 연결되는 것을 특징으로 하는 밀리웰 또는 마이크로웰 구조를 사용하는 바이오 센서.A biosensor using a milliwell or microwell structure, characterized in that the plurality of sensing units are separated from each other through a barrier rib, and are electrically connected to each other through a connection port electrically coupleable to the connection module.
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JPS6454345A (en) * | 1987-08-26 | 1989-03-01 | Matsushita Electric Ind Co Ltd | Biosensor |
KR20020088521A (en) * | 2001-05-18 | 2002-11-29 | 주식회사 아이센스 | Biosensors with porous chromatographic membranes and enhanced sampling capability |
JP2003532116A (en) * | 2000-04-25 | 2003-10-28 | オンコプローブ・リミテッド | Electrochemical sensing |
JP2005164387A (en) * | 2003-12-02 | 2005-06-23 | Osaka Industrial Promotion Organization | Dna chip and biosensor using it |
JP2018515745A (en) * | 2015-05-12 | 2018-06-14 | インディアン インスティテゥート オブ サイエンスIndian Institute Of Science | Apparatus and method for detecting creatinine and albumin / creatinine ratio |
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JPS6454345A (en) * | 1987-08-26 | 1989-03-01 | Matsushita Electric Ind Co Ltd | Biosensor |
JP2003532116A (en) * | 2000-04-25 | 2003-10-28 | オンコプローブ・リミテッド | Electrochemical sensing |
KR20020088521A (en) * | 2001-05-18 | 2002-11-29 | 주식회사 아이센스 | Biosensors with porous chromatographic membranes and enhanced sampling capability |
JP2005164387A (en) * | 2003-12-02 | 2005-06-23 | Osaka Industrial Promotion Organization | Dna chip and biosensor using it |
JP2018515745A (en) * | 2015-05-12 | 2018-06-14 | インディアン インスティテゥート オブ サイエンスIndian Institute Of Science | Apparatus and method for detecting creatinine and albumin / creatinine ratio |
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