KR20140140502A - Module for detecting substances by electric chemical and apparatus for detecting substances with the same - Google Patents

Module for detecting substances by electric chemical and apparatus for detecting substances with the same Download PDF

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KR20140140502A
KR20140140502A KR1020140063679A KR20140063679A KR20140140502A KR 20140140502 A KR20140140502 A KR 20140140502A KR 1020140063679 A KR1020140063679 A KR 1020140063679A KR 20140063679 A KR20140063679 A KR 20140063679A KR 20140140502 A KR20140140502 A KR 20140140502A
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South Korea
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electrode
target material
active electrode
flow path
output
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KR1020140063679A
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Korean (ko)
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조원우
유경화
고경옥
박종배
이준옥
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주식회사 캔티스
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Priority to PCT/KR2014/004739 priority Critical patent/WO2014193154A1/en
Publication of KR20140140502A publication Critical patent/KR20140140502A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3273—Devices therefor, e.g. test element readers, circuitry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

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  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
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  • Urology & Nephrology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Microbiology (AREA)
  • Food Science & Technology (AREA)
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  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

A material detecting module and a material detecting device having the material detecting module are disclosed. A material detecting module according to an embodiment of the present invention includes an inlet flow path formed at one side and a sample containing a target material into which the sample flows, a discharge flow path formed at the other side of the sample and containing a target material, A chamber formed between the flow paths and including an electrode sensor receiving groove communicating with the inlet flow path and the exhaust flow path, respectively; A reference electrode formed on one surface of the base substrate and provided so as to allow the target material to pass therethrough when the sample flows in through the inflow channel, and a reference electrode formed on one surface of the base substrate, And an active electrode arranged to acquire a target material when the sample is introduced through the inlet flow path.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to an electrochemical material detecting module and a material detecting device having the same. BACKGROUND ART [0002]

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a detection device, and more particularly, to a substance detection module capable of electrochemically detecting biomolecules and a substance and a substance detection device having the same.

Generally, a biosensor is used to detect a specific biological substance. Here, a biosensor means a tool or a device that is manufactured to measure the presence or amount of a specific biological substance. A biosensor is a signal that recognizes electrochemical changes, changes in thermal energy, changes in fluorescence or color, etc., which occur in the reaction between a specific biological substance and a biological element (for example, enzyme, antigen, antibody, biochemical substance) And a conversion device. Thus, the biosensor can quickly and easily analyze a complex substance using biological elements capable of recognizing a biological substance, and can selectively detect a substance to be analyzed. Such biosensors can reduce the limit of disease detection related to the diagnosis of diseases in the medical field. Such general biosensors have the following disadvantages.

In order to analyze a specific substance, a conventional biosensor is provided with an electrode serving as a reference and an electrode through which a substance to be analyzed is cultured. In this case, the electrode is manufactured through a process similar to the semiconductor process and is subjected to a complicated process. And, the base substrate required to produce the electrode, that is, the glass substrate or the silicon-based substrate, is expensive. Therefore, there is a difficulty in cost competitiveness in commercializing a conventional biosensor in large quantities. In addition, the conventional biosensor has a problem in that reproducibility and accuracy are degraded when measured by various environmental variables.

Korean Unexamined Patent Application Publication No. 10-2006-0089464 (Aug. 2006)

An embodiment of the present invention is to provide a substance detection module and a substance detection device having the substance detection module capable of increasing the detection reliability of a target substance.

An embodiment of the present invention is to provide a substance detection module capable of increasing the sensitivity of detection of a target substance and a substance detection apparatus having the same.

An embodiment of the present invention is to provide a material detecting module and a material detecting device having the same that can simplify the manufacturing process.

A material detecting module according to an embodiment of the present invention includes an inlet flow path formed at one side and a sample containing a target material into which the sample flows, a discharge flow path formed at the other side of the sample, A chamber formed between the discharge flow paths and including an electrode sensor storage groove communicating with the inflow flow path and the discharge flow path, respectively; A reference electrode formed on one surface of the base substrate and adapted to allow the target material to pass when the sample flows through the inflow channel; And an active electrode formed to be spaced apart from the reference electrode and adapted to acquire the target material when the sample flows through the inflow channel.

Wherein the reference electrode includes mutually opposing first reference electrodes and second reference electrodes in the form of interdigitated electrodes and the active electrode includes mutually opposing first active electrodes and second active electrodes in the form of interdigitated electrodes And the reference electrode and the active electrode may be formed identically.

The active electrode may be provided to obtain the target material between the first active electrode and the second active electrode.

A receptor may be provided between the first active electrode and the second active electrode to couple the target material with the target material.

The material detection module may further include: a signal generator for applying an input signal to the reference electrode and the active electrode, respectively; And a comparator comparing the first output signal output from the reference electrode and the second output signal output from the active electrode to detect a signal by the target material.

The comparing unit may amplify a difference signal between the first output signal and the second output signal to detect a signal by the target material.

The electrode sensor includes: a first input pad provided on the other surface of the base substrate and electrically connected to the first reference electrode, to which the input signal is applied; A first output pad provided on the other surface of the base substrate and electrically connected to the second reference electrode and outputting the first output signal; A second input pad provided on the other surface of the base substrate and electrically connected to the first active electrode, to which the input signal is applied; And a second output pad provided on the other surface of the base substrate and electrically connected to the second active electrode and outputting the second output signal.

The chamber includes a first through hole exposing the first input pad and the first output pad to the outside; And a second through hole exposing the second input pad and the second output pad to the outside.

The base substrate may be made of a biocompatible insulating material.

The electrode sensor may be made of FPCB (Flexible Printed Circuit Board).

Wherein the inflow channel is branched into a first inflow channel corresponding to the reference electrode and a second inflow channel corresponding to the active electrode, and a sample containing the target material is supplied to the first inflow channel and the second inflow channel, To the reference electrode and the active electrode.

Wherein the chamber includes: a base portion on which the electrode sensor receiving groove is formed; And a cover which is coupled to the base portion at an upper portion of the base portion so as to be hermetically sealed and in which the inflow passage and the discharge passage are formed on a surface facing the base portion.

A material detecting apparatus according to an embodiment of the present invention includes: a material detecting module; An injection port for injecting a sample containing the target material into the material detection module; An outlet through which the sample containing the target material is discharged through the material detecting module; And a display unit for displaying a detection result of the material detection module.

According to the embodiment of the present invention, by forming the environment of the reference electrode and the active electrode in the same manner and shielding the electrode sensor from the outside, it is possible to accurately detect the target material without being influenced by various environmental variables, . By using an FPCB (Flexible Printed Circuit Board) as the electrode sensor, it is possible to manufacture a material compatible with the biomaterial so that a heterogeneous element is not involved in the detection environment of the target material, Can be made more easily and the manufacturing cost can be lowered. In addition, by forming the receptor in the region between the pair of interdigitated electrodes in the active electrode region, it is possible to minimize the noise at the time of detecting the target material and measure the impedance change amount at a low current, The sensitivity of the substance detection can be increased.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a view showing a substance detecting apparatus according to an embodiment of the present invention; FIG.
2 is a diagram showing a material detection module in a material detection apparatus according to an embodiment of the present invention.
3 is a view showing an electrode sensor in a material detecting apparatus according to an embodiment of the present invention.
4 is a view showing a manufacturing process of an electrode sensor in a material detecting apparatus according to an embodiment of the present invention;
5 is a diagram showing a state in which a material detection module detects a substance in a material detection apparatus according to an embodiment of the present invention;
6 is a view illustrating a case where a receptor is formed on a pair of interdigitated electrodes in an active electrode region of a material detection module according to an embodiment of the present invention
7 is a view illustrating a case where a receptor is formed between a pair of interdigitated electrodes in an active electrode region of a material detection module according to an embodiment of the present invention
8 is a block diagram showing a configuration of a substance detection apparatus according to an embodiment of the present invention.
9 is a graph showing a state in which A-Beta protein is detected by the substance detecting apparatus according to an embodiment of the present invention

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a specific embodiment of a substance detection module and a substance detection device having the substance detection module of the present invention will be described with reference to FIG. 1 to FIG. However, this is an exemplary embodiment only and the present invention is not limited thereto.

In the following description, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions of the present invention, and may be changed according to the intention or custom of the user, the operator, and the like. Therefore, the definition should be based on the contents throughout this specification.

The technical idea of the present invention is determined by the claims, and the following embodiments are merely a means for efficiently describing the technical idea of the present invention to a person having ordinary skill in the art to which the present invention belongs.

1 is a view showing a material detecting apparatus according to an embodiment of the present invention.

Referring to FIG. 1, the substance detection apparatus 10 may include an injection port 20, an outlet 30, a substance detection module 40, and a display unit 50.

The injection port 20 may be a passage through which a sample containing a substance to be detected (hereinafter, referred to as a target substance) may be introduced. Here, the sample containing the target substance may be a fluid in which a solvent and a target substance (for example, cells, enzymes, antigens, antibodies, biochemicals, etc.) are mixed. In addition, the sample containing the target material may be a gas containing a target material such as dioxin, TNT (Trinitrotoluene), DNT (Dinitro toluene), or the like.

The discharge port 30 may be a passage through which the sample containing the target material introduced through the injection port 20 is discharged to the outside through the material detection module 40. At this time, a hose, a tube, or the like is attached to the injection port 20 and the discharge port 30, respectively, so that a sample containing the target material can be injected or discharged.

The material detection module 40 can detect the target material while the sample containing the target material flows into the inlet 20 and is discharged to the outlet 30. [ An input signal may be applied to the material detection module 40 to detect the target material when the sample containing the target material is introduced through the injection port 20. [ In the material detection module 40, an electrode sensor 140 is provided. The applied input signal may be transmitted to the electrode sensor 140. The electrode sensor 140 can electrically measure and output physical and chemical changes due to the target material passing through the electrode sensor 140. Hereinafter, the electrode sensor 140 will be described in detail with reference to FIG.

The display unit 50 may display the information detected and analyzed through the material detection module 40 on a screen so that the user can confirm the information.

The material detecting apparatus 10 can detect and analyze a target material in real time while injecting a sample containing the target material. In addition, the material detecting apparatus 10 can be manufactured in a small size to enhance portability. That is, the substance detection apparatus 10 can be made portable. Hereinafter, the description of the substance detection apparatus 10 will be described in detail with reference to FIG. 2 through FIG.

2 is a diagram illustrating a material detection module in a material detection apparatus according to an embodiment of the present invention. 2 (a) is a view showing a state in which the base portion 242 and the cover 252 are engaged with each other in the material detecting module, and FIG. 2 (b) Fig.

Referring to FIG. 2, the material detection module 40 may be provided to allow a sample containing a target material to flow in and out. The material detection module 40 serves to detect a target material in a sample containing the target material. The material detection module 40 may include a chamber 240 and an electrode sensor 140.

The chamber 240 includes a base portion 242 and a cover 252. An electrode sensor accommodating groove 244, an inflow passage 246, a discharge passage 248, and a through hole 250 may be formed in the base portion 242.

The electrode sensor receiving groove 244 is provided to receive the electrode sensor 140. The electrode sensor receiving groove 244 may have a size corresponding to that of the electrode sensor 140. Here, when the electrode sensor 140 is received in the electrode sensor receiving groove 244, it is possible to seal the electrode sensor 140 using an adhesive or a sealing resin so that a gap is not formed between the electrode sensor receiving groove 244 and the electrode sensor 140 have. However, the present invention is not limited thereto, and various other methods can be used.

The inlet flow path 246 may be provided at one side of the electrode sensor receiving groove 244. The inflow passage 246 may include a first inflow passage 246a and a second inflow passage 246b. The inlet flow path 246 extends straight from the one end of the base portion 242 to the other end direction of the base portion 242 by a predetermined length and branches from the distal end portion of the straight line to the one end of the electrode sensor 140 . Here, the two branching portions may be the first inflow passage 246a and the second inflow passage 246b, respectively. The first inlet flow path 246a may be connected to one side of the electrode sensor 140 in the direction in which the reference electrode 144 is formed. The second inlet flow path 246b may be connected to one side of the electrode sensor 140 in the direction in which the active electrode 146 is formed. A sample containing a target material can be simultaneously introduced into the reference electrode 144 and the active electrode 146 through the first inlet flow path 246a and the second inlet flow path 246b. To this end, when the sample containing the target material is injected into the material detection module 40, the working pressure of the pump can be controlled to be constant, and the flow rate of the sample containing the target material and the injection amount per hour can be constantly controlled. The inflow channel 246 may be a channel through which the sample containing the target material flows by being formed in the base portion 242 while being grooved.

The discharge passage 248 may be provided on the other side of the electrode sensor receiving groove 244. The discharge passage 248 may include a first discharge passage 248a and a second discharge passage 248b. The discharge passage 248 extends straight from the other side of the base portion 242 to one side of the base portion 242 by a predetermined length and branches from the end portion of the straight line to the other end of the straight line to be connected to the other side of the electrode sensor 140 . Here, the two branching portions may be the first discharge flow path 248a and the second discharge flow path 248b, respectively. The first discharge flow path 248a may be connected to the other side of the electrode sensor 140 in the direction in which the reference electrode 144 is formed. The second discharge flow path 248b may be connected to the other side of the electrode sensor 140 in the direction in which the active electrode 146 is formed. The sample containing the target material can be discharged to the outside through the first discharge flow path 248a and the second discharge flow path 248b. The discharge passage 248 may be formed as a groove in the base portion 242 so that a sample containing the target material may be discharged.

The through hole 250 may be formed in the base portion 242 where the electrode sensor receiving groove 244 is formed. For example, the through hole 250 may include a first input pad 156 and a first output pad 158, a second input pad 160, and a second input pad 160 formed on the lower surface of the base substrate 142 of the electrode sensor 140. [ 2 output pads 162, respectively. The first input pad 156 and the second input pad 160 are electrically connected to the first output pad 158 and the second input pad 160 through the through hole 250, To obtain a signal output from the second output pad 162.

The cover 252 may be engaged with the base portion 242 at an upper portion of the base portion 242. The cover 252 may be provided in a size and shape corresponding to the base portion 242. The cover 252 and the base portion 242 can be hermetically sealed off from the outside. Since the sample containing the target material is simultaneously introduced into the reference electrode 144 and the active electrode 146 in the state where the substance detection module 40 is shielded from the outside, Can be minimized.

Grooves corresponding to the inflow passage 246 and the discharge passage 248 of the base portion 242 may be formed on the lower surface of the cover 252 (that is, the surface facing the base portion 242). In this case, the grooves formed on the lower surfaces of the inflow passage 246 and the discharge passage 248 of the base portion 242 and the cover 252 may be in the form of a tube to form a passage through which the substance to be detected may be introduced .

Although the electrode sensor storage groove 244, the inflow passage 246 and the discharge passage 248 are formed in the base portion 242 in this embodiment, the present invention is not limited thereto. And the inflow passage 246 and the discharge passage 248 may be formed in the cover 252. [ In this case, there is no step difference between the inlet flow path 246 and the discharge flow path 248 and the electrode sensor 140 housed in the electrode sensor receiving groove 244. As a result, the sample containing the target material can be injected as much as possible into the reference electrode 144 and the active electrode 146 at the same time.

The electrode sensor 140 can be received and fixed in the electrode sensor receiving groove 244 of the base portion 242. The electrode sensor 140 may quantitatively detect a target material through signals output from the reference electrode 144 and the active electrode 146 when a sample containing the target material is introduced. Hereinafter, the electrode sensor 140 will be described in detail with reference to FIG.

3 is a view showing an electrode sensor of a material detecting apparatus according to an embodiment of the present invention.

Referring to FIG. 3, the electrode sensor 140 may be manufactured using an FPCB (Flexible Printed Circuit Board). However, the present invention is not limited thereto, and the electrode sensor 140 may be manufactured using a PCB (Printed Circuit Board). The electrode sensor 140 includes a base substrate 142, a reference electrode 144, an active electrode 146, a first input line 148, a second input line 152, a first output line 150, And may include an output line 154, a first input pad 156, a second input pad 160, a first output pad 158 and a second output pad 162.

The base substrate 142 may be made of a biocompatible insulating material. The biocompatible insulation material is made of glass, tritan, polyimide, polypropylene, polyethylene, polyethylene, polycarbonate, polystyrene, and biopolymer. Can be used. For example, PI (polyimide) film has a transparent property and is a biocompatible material free from harmful components, and has the following advantages. First, since the PI film has a transparent property, the user can visually confirm the target substance directly using a microscope. Further, since the PI film is not a harmful substance, accurate detection can be performed since it does not affect the target substance in detecting the target substance.

The reference electrode 144 may be formed on the upper surface of the base substrate 142. The reference electrode 144 may include a first reference electrode 144a and a second reference electrode 144b. The first reference electrode 144a and the second reference electrode 144b may be formed as comb-like electrodes. The first reference electrode 144a and the second reference electrode 144b may be interdigitated and may be spaced apart from each other to form a pair. The distance between the first reference electrode 144a and the second reference electrode 144b may be several micrometers to several hundreds of micrometers. A first input line 148 is connected to one side of the first reference electrode 144a and an input signal may be applied through the first input line 148. [ A first output line 150 may be connected to one side of the second reference electrode 144b and an output signal may be output through the first output line 150. The surface of the reference electrode 144 may be subjected to a separate surface treatment so as not to react with the target material. That is, the reference electrode 144 may be provided so that the target material passes through (pass through) the reference electrode 144 when a sample containing the target material is introduced.

The active electrode 146 may be formed opposite to the reference electrode 144 on the upper surface of the base substrate 142. The active electrode 146 may include a first active electrode 146a and a second active electrode 146b. The first active electrode 146a and the second active electrode 146b may be formed as comb-shaped electrodes, respectively. The first active electrode 146a and the second active electrode 146b may be interdigitated and may be spaced apart from each other by a predetermined distance. The interval between the first active electrode 146a and the second active electrode 146b may be several micrometers to several hundred micrometers. A second input line 152 is connected to one side of the first active electrode 146a and an input signal such as a signal applied to the first input line 148 through the second input line 152 may be applied . A second output line 154 is connected to one side of the second active electrode 146b and an output signal may be output through the second output line 154. [ A receptor 182 may be coated on the active electrode 146 region. The receptor 182 serves to couple with a specific target material and to fix the target material to the active electrode 146 region. The type of the receptor 182 may differ depending on the target material to be detected. However, it is not so limited, and the active electrode 146 region may be surface treated in various ways to obtain a target material. The signal output from the second output line 154 of the active electrode 146 is different from the signal output from the first output line 150 of the reference electrode 144 by the receptor 182 reacting with the target material. That is, the reference electrode 144 is provided not to be coupled with the target material, and the active electrode 146 is provided to fix the target material in combination with the target material, Signal) and the signal output from the active electrode 146 (that is, a signal to be compared) to detect a target material. At this time, the target material can be quantitatively detected through the difference between the reference signal and the comparison target signal. For example, the difference between the reference signal and the compared signal may be proportional to the amount of target material immobilized in the active electrode 146 region. In this case, the target material can be quantitatively detected through the difference between the reference signal and the comparison target signal.

Meanwhile, the reference electrode 144 and the active electrode 146 may be formed in the same size and shape. For example, when the reference electrode 144 and the active electrode 146 are each formed of a pair of interdigit electrodes, the interdigit electrode thickness, length, and width and the interval between the interdigitated electrodes may be formed in the same manner . In this case, it is possible to compare the reference signal output from the reference electrode 144 with the comparison target signal output from the active electrode 146 under the same conditions and environment.

Here, the reference electrode 144 and the active electrode 146 are formed in the base portion 242, respectively, but the present invention is not limited thereto. For example, a pair of the reference electrode 144 and the active electrode 146 may form one cell, and a plurality of cells may be formed in an array form in the base portion 242. Further, one reference electrode 144 may be formed, and a plurality of active electrodes 146 may be formed.

The first input line 148 and the first output line 150 and the second input line 152 and the second output line 154 extend to the lower surface of the base substrate 142 and are formed in a pad shape . That is, a first input pad 156 and a first output pad 158 may be formed on the lower surface of the base substrate 142 to be connected to the first input line 148 and the first output line 150, respectively. A second input pad 160 and a second output pad 162 connected to the second input line 152 and the second output line 154 may be formed on the lower surface of the base substrate 142. The first input pad 156, the second input pad 160, the first output pad 158, and the second output pad 162 may be electrically connected to the material detection device 10, respectively.

4 is a view showing a manufacturing process of an electrode sensor in a material detecting apparatus according to an embodiment of the present invention.

Referring to FIG. 4, the electrode sensor 140 may be manufactured using FPCB. First, the FPCB has a structure in which a copper foil layer 170 is laminated on a base substrate 142 (for example, a PI film or the like) (FIG. 4 (a)). Next, an electrode pattern can be formed on the base substrate 142 by, for example, an exposure and etching process or a photolithography process. Here, the electrode pattern includes a reference electrode 144, an active electrode 146, a first input line 148, a second input line 152, a first output line 150, and a second output line 154 . The copper foil layer 170 is left on the electrode pattern and the remaining portion of the copper foil 170 except for the electrode pattern is removed by the exposure and etching process or the photolithography process (FIG. 4B) . Next, the plating layer 172 may be formed by performing a plating process on the electrode pattern. At this time, the copper foil layer 170 in the electrode pattern portion becomes a seed layer and can be easily plated (Fig. 4 (c)). Here, the plating layer 172 may be made of gold (Au). However, the present invention is not limited thereto. For example, the plating layer 172 may be formed of silver (Ag) or platinum (Pt).

In the embodiment of the present invention, the electrode sensor 140 is manufactured using the FPCB, and the electrode can be easily formed by performing the plating process directly using the copper foil layer 170 as the seed layer as the electrode pattern. In addition, instead of using expensive raw materials such as wafers or glass substrates used for forming electrodes in a semiconductor process, the manufacturing cost can be lowered by using a low cost FPCB. Since the PI film of the FPCB is not a harmful substance, it can be used without being affected by external parameters such as the material of the base substrate 142 and the like. Since the PI film has a transparent property, the user can directly confirm the biomaterial in the substance detection device 10 with the naked eye using a microscope. Here, the electrode sensor 140 is manufactured through the FPCB process. However, the present invention is not limited thereto. The electrode sensor 140 may be manufactured through a printed circuit board (PCB) process.

5 is a diagram showing a state in which a material detection module detects a substance in a material detection apparatus according to an embodiment of the present invention.

Referring to FIG. 5, it can be seen how the target material 180 reacts with the reference electrode 144 and the active electrode 146 when a sample containing the target material 180 is inserted into the material detection module 40 .

A sample containing the target material 180 may be introduced into the material detection module 40 through the injection port 20. The sample including the target material 180 may flow into the reference electrode 144 and the active electrode 146 of the electrode sensor 140 through the inflow channel 246, respectively. A sample including the target material 180 may be simultaneously introduced into the reference electrode 144 and the active electrode 146. At this time, input signals may be applied to the first input line 148 of the reference electrode 144 and the second input line 152 of the active electrode 146, respectively.

The reference electrode 144 is not coated with the receptor 182 that reacts with and reacts with the target material 180 so that the target material 180 reacts at the reference electrode 144 And is discharged to the outside through the discharge flow path 248.

The active material 146 is coated with a receptor 182 that reacts with and binds to the target material 180 so that the target material 180 is electrically coupled to the active electrode 146, (182). That is, the active material 146 is specifically bound to the target material 180 by the receptor 182. In this case, the target material 180 coupled with the receptor 182 interferes with the ion exchange between a pair of interdigitated electrodes of the active electrode 146, thereby increasing the interelectrode impedance.

Here, the difference in impedance between the signal output from the reference electrode 144 and the signal output from the active electrode 146 can be measured to detect the amount (or concentration) of the target material.

On the other hand, in the active electrode 146 region, the receptor 182 can be formed in a region between a pair of interdigitated electrodes. In this case, compared to the case where the receptor 182 is formed on the pair of interdigit electrodes, the noise can be minimized and the impedance change amount can be measured at low current. As a result, a high-resolution current measurement becomes possible, and the sensitivity of detecting the target substance of the electrode sensor 140 can be increased. This will be described in detail with reference to FIG. 6 and FIG.

6 is a view illustrating a case where a receptor is formed on a pair of interdigitated electrodes in an active electrode region of a material detection module according to an embodiment of the present invention.

Referring to FIG. 6A, a receptor 182 may be formed on the first active electrode 146a and the second active electrode 146b. In this case, the target material 180 is coupled to the receptor 182 on the first active electrode 146a and the second active electrode 146b. Here, when an input signal is applied to the first active electrode 146a through the second input line 152, the input signal flows along the first active electrode 146a, and the first active electrode 146a and the second active electrode 146b And flows through the second active electrode 146b via the electrode 146b and is output through the second output line 154. [

6B is a diagram schematically showing the impedance between the first active electrode 146a and the second active electrode 146b. 6 (b), the impedance between the first active electrode 146a and the second active electrode 146b is higher than the impedance between the first active electrode 146a and the second active electrode 146b, (Z solvent 1) by the solvent (Z solvent 1) on the first active electrode 146a and the second active electrode 146b by the first active electrode 146 and the first active electrode 146a, And the impedance due to the solvent (Z solvent 2) buried between the second active electrodes 146b. Here, the solvent refers to the solvent contained in the sample injected into the material detection module 40.

The impedance (Z target 1) caused by the target substance 180 (Z target material) and the solvent (Z solvent 1) on the first active electrode 146a and the second active electrode 146b are connected in series. The impedance (Z solvent 2) caused by the solvent between the first active electrode 146a and the second active electrode 146b is lower than the impedance (Z target material) by the target material 180 and the first active electrode 146a. (Z solvent 1) caused by the solvent on the first active electrode 146a and the second active electrode 146b.

Here, the target substance 180 can be quantitatively detected by measuring the impedance (Z target material) by the target material 180 (i.e., measuring the current flowing through the Z target material). The first active electrode 146a and the second active electrode 146b are connected in series to the impedance (Z target material) by the target material 180. The impedance (Z solvent 1) (Z target material) by the target material 180 because a current (for example, several thousand times) that is much higher than the current flowing through the impedance (Z target material) .

7 is a view illustrating a case where a receptor is formed between a pair of interdigitated electrodes in an active electrode region of a material detection module according to an embodiment of the present invention.

Referring to FIG. 7A, a receptor 182 may be formed between the first active electrode 146a and the second active electrode 146b. In this case, the target material 180 is coupled with the receptor 182 between the first active electrode 146a and the second active electrode 146b.

7B is a diagram schematically showing the impedance between the first active electrode 146a and the second active electrode 146b. 7 (b), the impedance between the first active electrode 146a and the second active electrode 146b is higher than the impedance between the first active electrode 146a and the second active electrode 146b, (Z target material) and impedance (Z solvent) due to the solvent. Here, the impedance due to the solvent (Z solvent)

May include an impedance due to the solvent on the first active electrode 146a and the second active electrode 146b and an impedance due to the solvent between the first active electrode 146a and the second active electrode 146b have. Impedance (Z target material) by the target material 180 and impedance (Z solvent) by the solvent are connected in parallel.

As such, by immobilizing the target material 180 between the first active electrode 146a and the second active electrode 146b, by the solvent in series with the impedance (Z target material) by the target material 180 It is possible to sensitively detect only the current flowing through the impedance (Z target material) caused by the target material 180. [ The impedance (Z solvent) caused by the solvent connected in parallel to the impedance (Z target material) by the target material 180 can be removed by using the reference electrode 144. That is, since an impedance component due to solvent exists between a pair of interdigitated electrodes of the reference electrode 144 and a pair of interdigitated electrodes, the output from the reference electrode 144 from the output signal from the active electrode 146 The impedance (Z solvent) due to the solvent connected in parallel to the impedance (Z target material) by the target material 180 can be removed. In this case, the current flowing in the impedance (Z target material) caused by the target material 180 can be more accurately and precisely detected.

8 is a block diagram showing the configuration of a substance detection apparatus according to an embodiment of the present invention.

8, the substance detection apparatus 10 may include a signal generation unit 300, a reference electrode 144, an active electrode 146, and a comparison unit 340.

The signal generator 300 applies the same input signal 310 to the reference electrode 144 and the active electrode 146 when a sample containing the target material 180 is introduced into the material detection module 40 . The first output signal 320 and the second output signal 330 are output from the reference electrode 144 and the active electrode 146 to the comparison unit 340 by a change depending on the sample including the target material 180 Respectively. Since the receptor 182 is not coated in the region of the reference electrode 144 and the receptor 182 is coated in the region of the active electrode 146, the first output signal 320 and the second output signal 330 There will be a difference depending on the target material 180.

The comparing unit 340 may compare the first output signal 320 with the second output signal 330 to detect a current signal by the target material 180. [ For example, the comparing unit 340 may amplify the difference signal between the first output signal 320 and the second output signal 330 to detect a current signal (or impedance) by the target material 180. Here, by using the current signal by the target material 180, the amount or the concentration of the target material 180 can be measured.

FIG. 9 is a graph showing a state in which A-Beta protein is detected by a substance detection device according to an embodiment of the present invention.

9, an antibody (i.e., a receptor) that binds to the A-Beta protein was surface-treated between the first active electrode 146a and the second active electrode 146b in the region of the active electrode 146. [ Then, A-Beta protein was injected at a constant interval of 1 pg (pico gram) / ml, 10 pg / ml, 100 pg / ml and 1000 pg / ml. Here, when the 1 pg / ml A-Beta protein is injected, the resistance value increases from 0 to 20000 ohms. This shows that the electrode sensor 140 sensed the target material (i.e., the A-Beta protein) very sensitively. As the concentration of A-Beta protein increases, the resistance value increases. Therefore, by measuring the resistance value (impedance) according to the target material, it becomes possible to accurately detect the amount or the concentration of the target material.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, I will understand. Therefore, the scope of the present invention should not be limited to the above-described embodiments, but should be determined by equivalents to the appended claims, as well as the appended claims.

10: substance detection device 20: inlet
30: exhaust port 40: material detection module
50: Display section 140: Electrode sensor
142: base substrate 144: reference electrode
144a: first reference electrode 144b: second reference electrode
146: active electrode 146a: first active electrode
146b: second active electrode 148: first input line
150: first output line 152: second input line
154: second output line 156: first input pad
158: first output pad 160: second input pad
162: second output pad 170: copper foil layer
172: Plating layer 180: Target material
182: Receptor 240: Chamber
242: base portion 244: electrode sensor storage groove
246: Inflow channel 246a: First inflow channel
246b: second inflow passage 248:
248a: first discharge flow passage 248b: second discharge flow passage
250: through hole 252: cover
300: Signal generator 310: Input signal
320: first output signal 330: second output signal
340:

Claims (13)

An inlet flow path formed at one side of the inlet and into which the sample containing the target material flows, a discharge flow path formed at the other side of the sample flow path through which the sample containing the target material is discharged, and a flow path formed between the inlet flow path and the discharge flow path, A chamber including an electrode sensor receiving groove communicating with the flow path, respectively; And
A reference electrode formed on one surface of the base substrate and provided so as to allow the target material to pass when the sample flows through the inflow channel; And an active electrode formed spaced apart from the electrode and adapted to acquire the target material when the sample flows through the inflow channel.
The method according to claim 1,
Wherein the reference electrode includes mutually opposing first reference electrodes and second reference electrodes in the form of interdigitated electrodes,
Wherein the active electrode comprises mutually opposing first and second active electrodes in the form of interdigitated electrodes,
Wherein the reference electrode and the active electrode are formed identically.
3. The method of claim 2,
Wherein the active electrode comprises:
And to obtain the target material between the first active electrode and the second active electrode.
The method of claim 3,
And a receptor is provided between the first active electrode and the second active electrode to bind the target material and fix the target material.
3. The method of claim 2,
The substance detection module includes:
A signal generator for applying an input signal to the reference electrode and the active electrode, respectively; And
And a comparator for comparing a first output signal output from the reference electrode and a second output signal output from the active electrode to detect a signal by the target material.
6. The method of claim 5,
Wherein,
And detects a signal by the target material by amplifying a difference signal between the first output signal and the second output signal.
6. The method of claim 5,
The electrode sensor includes:
A first input pad provided on the other surface of the base substrate and electrically connected to the first reference electrode, to which the input signal is applied;
A first output pad provided on the other surface of the base substrate and electrically connected to the second reference electrode and outputting the first output signal;
A second input pad provided on the other surface of the base substrate and electrically connected to the first active electrode, to which the input signal is applied; And
And a second output pad provided on the other surface of the base substrate and electrically connected to the second active electrode, the second output pad outputting the second output signal.
8. The method of claim 7,
The chamber may comprise:
A first through hole exposing the first input pad and the first output pad to the outside; And
And a second through hole exposing the second input pad and the second output pad to the outside.
The method according to claim 1,
The base substrate includes:
Material detection module made of biocompatible insulation material.
The method according to claim 1,
The electrode sensor includes:
A material detection module made of FPCB (Flexible Printed Circuit Board).
The method according to claim 1,
Wherein the inflow channel comprises:
Wherein the sample is branched into a first inflow conduit corresponding to the reference electrode and a second inflow conduit corresponding to the active electrode, and a sample containing the target material flows through the first inflow conduit and the second inflow conduit, And being capable of being simultaneously introduced into the active electrode.
The method according to claim 1,
The chamber may comprise:
A base portion on the upper surface of which the electrode sensor receiving groove is formed; And
And a cover coupled to the upper portion of the base portion so as to be hermetically sealed with the base portion and having the inflow passage and the discharge passage formed on a surface facing the base portion.
A material detecting module according to any one of claims 1 to 12;
An injection port for injecting a sample containing the target material into the material detection module;
An outlet through which the sample containing the target material is discharged through the material detecting module; And
And a display unit for displaying a detection result of the substance detection module.
KR1020140063679A 2013-05-29 2014-05-27 Module for detecting substances by electric chemical and apparatus for detecting substances with the same KR20140140502A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017138696A1 (en) * 2016-02-12 2017-08-17 주식회사 캔티스 Detection method and electrochemical detection kit for aggregate form of protein, and diagnostic method and electrochemical diagnostic kit for diseases associated with abnormal protein aggregation by using same
WO2019208901A1 (en) * 2018-04-27 2019-10-31 재단법인 구미전자정보기술원 Electrochemical bio-marker detection method and device for diagnosing diabetes
US11448611B2 (en) * 2019-07-03 2022-09-20 Medtronic Minimed, Inc. Structurally reinforced sensor and method for manufacturing the same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017138696A1 (en) * 2016-02-12 2017-08-17 주식회사 캔티스 Detection method and electrochemical detection kit for aggregate form of protein, and diagnostic method and electrochemical diagnostic kit for diseases associated with abnormal protein aggregation by using same
WO2019208901A1 (en) * 2018-04-27 2019-10-31 재단법인 구미전자정보기술원 Electrochemical bio-marker detection method and device for diagnosing diabetes
US11448611B2 (en) * 2019-07-03 2022-09-20 Medtronic Minimed, Inc. Structurally reinforced sensor and method for manufacturing the same

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