WO2022176049A1 - 液柱分離デバイス、システム、及び方法 - Google Patents
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- WO2022176049A1 WO2022176049A1 PCT/JP2021/005855 JP2021005855W WO2022176049A1 WO 2022176049 A1 WO2022176049 A1 WO 2022176049A1 JP 2021005855 W JP2021005855 W JP 2021005855W WO 2022176049 A1 WO2022176049 A1 WO 2022176049A1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- B01D2221/00—Applications of separation devices
- B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
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- B01D2325/38—Hydrophobic membranes
Definitions
- the present invention relates to a liquid column generation method, particularly a liquid column separation device, system, and method, which are technologies related to biomeasurement.
- the nanopore DNA sequencer consists of a membrane with pores and a chamber with electrodes placed above and below the membrane. Nanopores, which are pores on the membrane, are the only channels that connect the two chambers separated by the membrane. When a voltage is applied by the electrodes in this configuration, an electric field is generated within the chamber such that electric lines of force pass through the pore, and the biomolecules are forced through the nanopore by the force of the electric field. When the biomolecules pass through the nanopore, the impedance changes due to the structure of the biomolecules. Therefore, the structure of the biomolecules can be identified by measuring the change in the current flowing between the two electrodes.
- the inventors instead of a chamber, the inventors opposed a membrane with nanopores to a substrate with electrodes, and formed columnar droplets (hereinafter referred to as liquid columns) between the nanopores and the electrodes.
- liquid columns formed columnar droplets (hereinafter referred to as liquid columns) between the nanopores and the electrodes.
- Patent Document 1 discloses a method for arraying a plurality of minute droplets in a device for biomolecular analysis.
- the device of Patent Document 1 is a device for single-molecule observation of a fluorescent dye.
- This device has a structure in which a first substrate having a plurality of minute hydrophilic wells in a hydrophobic surface faces a second substrate in parallel.
- an oil-based sealing liquid is sent between the substrates and then an oil-based sealing liquid is sent, the space between the substrates is filled with the oil-based sealing liquid, but the sample remains in the plurality of wells.
- the upper well becomes an independent reaction chamber.
- Patent Document 2 discloses a device capable of operating droplets sandwiched between two substrates using electrowetting, which is a phenomenon in which the surface wettability changes when an electric potential is applied to an electrode. ing.
- the substrate side with the electrodes has a water-repellent film, but the facing substrate has a hydrophilic film, and droplets can be retained at the position of the hydrophilic film.
- the inventors are studying a device that electrically isolates each nanopore by forming a liquid column between the nanopore and the electrode.
- the methods that have been reported so far do not sufficiently examine the conditions for generating a liquid column within the device. Since the object of Patent Document 1 is to observe a single molecule of a fluorescent dye, it is necessary to prevent sample liquid containing the fluorescent dye from remaining outside the reaction chamber, which is the observation area. If the sample liquid remains outside the reaction chamber, the fluorescence from the sample liquid remaining outside the reaction chamber becomes background light when observing a single molecule of the fluorescent dye in the reaction chamber. is inhibited. Therefore, it is not preferable for the sample liquid to form a liquid column in contact with the substrate including the wells and the opposing substrate.
- Patent Document 1 does not mention such conditions.
- a device in which the sample solution remains only in the well cannot be used for a nanopore DNA sequencer because the droplet cannot contact the nanopore or the electrode.
- Patent Document 2 requires a complicated process because it is necessary to provide a large number of electrodes and wiring. Furthermore, it is considered unsuitable for a nanopore DNA sequencer that measures minute changes in electric current because it is necessary to apply an electric potential to the electrodes in the device to move the droplet.
- An object of the present invention is to solve the above-mentioned problems and to provide a liquid column separation device, system, and method for producing a high-density liquid column array.
- the present invention provides a liquid column separation device comprising a first substrate and a second substrate arranged to face the first substrate with a predetermined gap therebetween. and a liquid feeding means for supplying two or more fluids between the first substrate and the second substrate, wherein the surface of the first substrate facing the second substrate is a hydrophobic hydrophobic region has a pattern in which a plurality of hydrophilic hydrophilic regions are arranged, the representative length of the hydrophilic region is greater than the predetermined interval, and the transfer is performed between the first substrate and the second substrate
- a liquid column separation device is provided that creates a liquid column in contact with a first substrate and a second substrate by flowing two or more immiscible fluids by liquid means.
- a liquid column in contact with the first base material and the second base material is produced by flowing the immiscible fluid, the first base material has an electrically independent first electrode in the hydrophilic region, and the second base material has an electrically independent first electrode.
- the material comprises a liquid column separation device having a membrane with nanopores in contact with the liquid column, a chamber containing an electrolyte solution in contact with the membrane, a second electrode in contact with the chamber, a measuring unit connected to the second electrode, and a measuring unit. and a control unit that controls the voltage applied to both electrodes according to the measurement result of, biomolecules are introduced into the liquid column, passed through the nanopore, and the time change of the ion current flowing between the electrodes is measured.
- This provides a liquid column separation system that detects the passage of biomolecules and analyzes the structural features of the biomolecules.
- the present invention provides a liquid column separation method, comprising: a first substrate; A base material and a liquid delivery means for supplying two or more fluids between a first base material and a second base material, wherein the surface of the first base material facing the second base material is hydrophobic A first base material and a second base material of a liquid column separation device having a pattern in which a plurality of hydrophilic regions are arranged in a hydrophobic region, and the representative length of the hydrophilic region is greater than a predetermined interval.
- a liquid column separation method for producing a liquid column in contact with a first substrate and a second substrate by flowing two or more immiscible fluids between them by means of a liquid feeding means.
- FIG. 2 is an explanatory diagram showing one configuration example of the liquid column separation device of Example 1;
- FIG. 4 is an explanatory diagram showing the process of generating a liquid column according to Example 1;
- FIG. 10 is a graph showing analysis results of droplets and liquid column generation conditions according to Example 1;
- FIG. 4 is a diagram showing droplets and a liquid column according to Example 1;
- FIG. 5 is a diagram showing a modification of the liquid column separation device of Example 1;
- FIG. 5 is a diagram showing a modification of the liquid column separation device of Example 1;
- FIG. 10 is a diagram showing a modified example of the liquid column separation device of Example 1, which has a structure in which a resistor is inserted in the hydrophilic region.
- FIG. 10 illustrates a process for creating hydrophobic regions on top of hydrophilic regions of a first substrate, according to a variant. It is a figure which shows the process of producing a pillar in the 2nd base material which concerns on a modification.
- Resist is a mixture mainly composed of resin (polymer), photosensitizer, additive, and solvent.
- 2 is a schematic diagram showing the configuration of a nanopore device according to Example 2.
- FIG. FIG. 10 is a schematic diagram showing the configuration of a nanopore device according to Example 3.
- Example 1 is an example of a liquid column separation device, system, and method for separating the liquid column connecting the first substrate and the second substrate.
- the configuration of the liquid column separation device 100 will be described with reference to FIG.
- FIG. 1(a) is a perspective view of the liquid column separation device 100
- FIG. 1(b) is a cross-sectional view taken along line A-A' of the perspective view.
- the liquid column separation device 100 is configured such that a peripheral edge member 105 is sandwiched between a first base material 101 and a second base material 102, and has an inlet 106 for injecting liquid into the liquid column separation device 100 and a liquid discharge port. It has an outlet 107 for
- the first base material 101 has a hydrophilic region 103 and a hydrophobic region 104 .
- the first substrate 101 has a film of the hydrophobic region 104 on the surface of the hydrophilic region 103 .
- the hydrophilic region 103 is a hydrophilic solid material such as glass or silicon oxide.
- Hydrophobic region 104 is composed of a hydrophobic substance. Examples include silane compounds, fluororesins, and hydrocarbon compounds. Examples of fluoropolymer resins include amorphous fluororesins. Amorphous fluororesin has advantages of high hydrophobicity and low toxicity to biological samples.
- a portion of the first base material 101 that is not covered with the hydrophobic region 104 becomes hydrophilic because the hydrophilic region 103 is exposed.
- the shape of the hydrophilic region 103 that is not covered with the hydrophobic region 104 may be circular, polygonal, or the like, for example.
- the hydrophobic region 104 is formed on the hydrophilic region 103, but conversely, the hydrophilic region may be formed on the hydrophobic region.
- a hydrophilic base material was used for the second base material 102 .
- glass, a silicon wafer having an oxide film, a hydrophobic material subjected to a hydrophilic surface treatment, or a resin laminated with a hydrophilic sheet may be used.
- a gap between the first base material 101 and the second base material 102 is supported by a peripheral edge member 105 .
- the material of the peripheral member 105 is not particularly limited, but examples thereof include a Teflon (registered trademark) sheet, a silicon sheet, a double-sided adhesive tape, dimethylpolysiloxane (PDMS), and the like.
- Fig. 1 (c) shows an example of how the liquid column separation device is used.
- a tube 108 is used to connect the injection port 106 and the solution sending means 109 , and the solution is sent from the solution sending means 109 .
- the liquid sending means may be a pump such as a syringe pump or a diaphragm pump, or may be manually sent using a syringe or a pipettor.
- FIG. 2 shows the process of producing the liquid column 112 with the liquid column separation device 100 of this embodiment.
- the hydrophilic first solution 110 is the dispersed phase
- the hydrophobic second solution 111 is the continuous phase.
- the hydrophilic first solution 110 is flowed into the liquid column separation device 100 from the inlet 106 . Excess first solution 110 flows out from outlet 107 .
- the hydrophobic second solution 111 is allowed to flow through the inlet 106 into the liquid column separation device 100 .
- a liquid column 112 is generated on the hydrophilic region 103 that is not covered with the hydrophobic region 104 .
- the hydrophilic liquid used in this embodiment is an electrolyte solution containing the biomolecules to be detected. From the viewpoint of the formation of the liquid column, the hydrophilic liquid must be hydrophilic regardless of the presence or absence of the biomolecules. is important.
- the hydrophilic liquid may contain a surfactant or the like. For example, Tween 20 and Triron-X100, which are nonionic surfactants that do not destroy the structure of biomolecules, are preferable.
- the hydrophobic liquid used in this embodiment is preferably silicone oil, mineral oil, or fluorine oil.
- Novec registered trademark
- a polymer having a perfluorocarbon structure for example, Novec (registered trademark), a polymer having a perfluorocarbon structure, Fluorinert (registered trademark) FC-40, Fluorinert (registered trademark) FC-43, and the like are used as the fluorine-based liquid.
- FIG. 3 shows the liquid column generation when D is the representative length of the hydrophilic region, and H is the distance between the hydrophilic surface of the first substrate and the second substrate, that is, the height of the peripheral member 105 in this embodiment.
- D is the representative length of the hydrophilic region
- H is the distance between the hydrophilic surface of the first substrate and the second substrate, that is, the height of the peripheral member 105 in this embodiment.
- It is a graph showing conditions, and fluid analysis was used for creation.
- the Y-axis is the ratio between the liquid flow rate and the center distance (pitch) of the hydrophilic region.
- a cross-sectional aspect ratio A of less than 1 is a condition in which a liquid column does not come into contact with the substrate.
- the condition where the cross-sectional aspect ratio A is 1 or more and less than 2 is a condition under which the formation of the liquid column is unstable.
- FIG. 4(a) and (b) are image diagrams of the generated liquid column 112 or droplet 113.
- FIG. 5 is another example of the shape of the liquid column separation device of this embodiment.
- the hydrophilic surface 103 of the second substrate 102 shown in FIG. 1 may not be separated from the adjacent liquid column because the liquid spreads over the surface. Therefore, as shown in FIG. 5, in order to constrain the range of the liquid column in contact with the surface of the second base material 102, the surface of the second base material 102 is also provided with a hydrophobic region 104' like the first base material 101. .
- the shape of this hydrophobic area is not necessarily the same as the hydrophobic area of the first substrate.
- the distance between the two substrates becomes narrower, so the pressure loss increases when the first solution and the second solution flow, making it difficult for the liquids to flow.
- the device structure shown in FIG. That is, it has a structure in which the portion of the convex structure 103' on the surface of the base material is a hydrophilic region.
- the distance between the surface of the hydrophilic region of the convex structure 103′ and the opposing substrate is narrowed, the distance between the surface of the hydrophilic region other than the convex structure and the substrate facing the substrate can be increased. increase can be suppressed.
- the method for producing the hydrophilic region of the protruding structure 103' is, for example, to heat-treat a silicon wafer to generate a hydrophilic silicon oxide layer, and use the above microfabrication techniques such as photolithography and etching to make it hydrophilic. A sexual region can be created. The etched portion of the hydrophilic silicon oxide layer becomes hydrophobic because the silicon is exposed.
- silicon rubber such as polydimethylsiloxane (PDMS), rubber materials such as natural rubber and fluororubber, and various plastics can be used.
- transparent materials such as quartz glass, float glass, calcium fluoride, silicon carbide, polymethyl methacrylate resin, and diamond, or opaque hard materials including metals such as ceramics, aluminum, and stainless steel can be used.
- FIGS. 8 and 9 show an example of the process of forming a hydrophobic region on the hydrophilic region of the first base material and the process of forming pillars on the second base material according to this modified example.
- the process of creating hydrophobic regions on the hydrophilic regions of the first substrate 101 includes silane coupling coating, hydrophobic coating , surface treatment, resist coating, exposure, development, dry etching, and resist removal to form a hydrophilic region.
- the second substrate 102 is coated with a resist (FIGS. 9A and 9B), and after exposure (FIG. 9C), the resist is By removing ((d) in the same figure) to form a resistor 114 and combining it with the first substrate 101 in FIG. 8 ((e) in the same figure), the resistor is placed in the hydrophilic region shown in FIG. be able to.
- the resist is a mixture containing resin (polymer), photosensitive agent, additive, and solvent as main components.
- the first substrate having a function for measuring a specific substance on the surface and the second substrate having a hydrophilic/hydrophobic pattern on the surface are opposed to each other.
- It is a column separation device, and has a supply hole for supplying fluid between substrates and a discharge port for discharging fluid, a function for measuring a specific substance on the first substrate, and a function for measuring a specific substance on the second substrate.
- the hydrophilic pattern is at a facing position, and the representative length of the hydrophilic region is larger than the distance between the first substrate and the second substrate, so that the hydrophilic region of the first substrate is opposed to the second substrate. can generate an independent liquid column in contact with
- Example 2 is an example of the measurement process using a nanopore DNA sequencer that combines a nanopore device and a liquid column separation device.
- An outline of the second embodiment will be described with reference to FIG.
- FIG. 10 is a schematic diagram showing the configurations of the liquid column separation device 100 and the nanopore device 200. As shown in FIG.
- the liquid column separation device 100 in this example used the same device as in Example 1, and as the second base material, the nanopore device 200 was used instead of the glass substrate.
- a nanopore device 200 includes a membrane 201 and a membrane-attached substrate 202 .
- the membrane 201 must have properties as an insulator, and although a silicon nitride film is used in this embodiment, a silicon oxide film, an organic substance, a polymer material, or the like may be used. Silver-silver chloride was used for the electrodes, but platinum, gold, or the like may also be used.
- the chamber 203 is configured so that the electrolyte solution 204 can be filled therein.
- the first electrode 205b is provided independently in each hydrophilic region of the first substrate at a position facing the second electrode 205a and the nanopore device 200, and the wiring 206, power supply and control/ It has a detection data acquisition unit 207 .
- the power supply and control/detection data acquisition unit 207 includes a high-output power supply, a processor, a memory, and a storage unit (not shown).
- the measurement process consists of two steps: nanopore formation and sample analysis.
- the formation of nanopores is performed in the following flow.
- the chamber 203 is filled with an electrolyte solution 204 .
- a liquid column of the aqueous solution is generated in contact with the membrane 201 and the first electrode 205b by the procedure shown in the first embodiment.
- a KCl aqueous solution was used as the first solution 110 and the electrolyte solution 204 in the chamber used to generate the liquid column.
- an aqueous solution of LiCl, NaCl, CaCl 2 , MgCl 2 , CsCl, or the like may be used.
- the first electrode 205 b contacts the first solution 110 and the second electrode 205 a contacts the electrolyte solution 204 .
- Nanometer-sized pores (nanopores) 208 are formed in the membrane 201 when a voltage is applied to the electrodes 205a and 205b.
- Example 2 Before creating a liquid column in the liquid column separation device 100 again, the inside of the liquid column separation device 100 is cleaned with a cleaning liquid and filled with the electrolyte solution together with the chamber 203 .
- a liquid column of an electrolyte solution containing biomolecules (such as DNA strands) is produced.
- a voltage is applied between the second electrode 205a in the chamber 203 and the independent first electrode 205b in contact with the liquid column.
- an electric field is generated around the nanopore, and the biomolecules are subjected to an electrostatic force by the electric field. Biomolecules pass through the nanopore due to electrostatic forces.
- biomolecules When the biomolecules pass through the nanopores, the biomolecules partially block the nanopores and change the resistance value of the nanopores, so that the current value detected between the two electrodes 205a and 205b changes.
- the structure of biomolecules is analyzed from the amount of change in current value.
- FIG. 11(a) shows a device based on the device of Example 2, in which a temperature control mechanism 209 capable of controlling the temperature cycle such as a Peltier element or a heat block is provided outside the first substrate.
- a temperature control mechanism 209 capable of controlling the temperature cycle such as a Peltier element or a heat block is provided outside the first substrate.
- the temperature of the liquid column containing biomolecules generated by the liquid column separation device 100 can be controlled, and the three temperature ranges required for PCR reaction (Polymerase Chain Reaction) can be quickly heated. • Allows cooling, holding a given temperature, and repeating up to a given number of iterations.
- temperature control may be performed for each liquid column by dividing the temperature control mechanism 209' for each hydrophilic well of the first substrate.
- Each cycle of DNA synthesis by PCR consists of three steps: denaturation, annealing, and extension.
- the target DNA sequence is synthesized by repeating this three-step PCR cycle several times.
- the properties of the DNA polymerase, the type of PCR buffer, and the complexity of the template DNA all influence the setup of these reaction conditions.
- 100 liquid column array device, 101: first substrate, 102: second substrate, 103, 103': hydrophilic regions, 104, 104': hydrophobic regions, 105: peripheral member, 106: inlet, 107 : outlet, 108: tube, 109: liquid feeding means, 110: first solution, 111: second solution, 112: liquid column, 113: droplet, 114, 114': resistor, 200: nanopore device, 201 : membrane, 202: substrate with membrane, 203: chamber, 204: electrolyte solution, 205a: electrode, 205b: electrode, 206: wiring, 207: power supply and control/detection data acquisition unit, 208: pores, 209, 209' : temperature control mechanism, D: representative lengths of hydrophilic region H, H': distance between first substrate, hydrophilic surface and second substrate A: cross-sectional aspect ratio.
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Abstract
Description
(0)最初の熱変性:95 °C 5分。加熱することにより、テンプレートの2本鎖DNAが分かれて1本鎖になる。
(1)熱変性:95 °C、30秒。
(2)アニーリング:60 °C、 60秒。プライマーがテンプレートに結合する。
(3)伸長:72 °C 、60秒。DNAポリメラーゼによって結合したプライマー部分からDNAが伸長する。
(1)(2)(3)の三つのステップを40サイクルで繰り返すことにより、目的領域のターゲットは指数的に増加する。
D:親水性領域の代表長さ
H、H‘:第1基材と親水性表面と第2基材の間隔
A:断面アスペクト比。
Claims (12)
- 液柱分離デバイスであって、
第1基材と、前記第1基材と所定の間隔を隔てて対向するように配置された第2基材と、前記第1基材と前記第2基材の間に2つ以上の流体を供給する送液手段と、を備え、
前記第1基材の前記第2基材と対向する表面は、疎水性の疎水性領域の中に、複数の親水性の親水性領域が配列したパターンを有し、前記親水性領域の代表長さが前記所定の間隔より大きく、
前記第1基材と前記第2基材の間に、前記送液手段により2つ以上の非相溶流体を流すことにより、前記第1基材及び前記第2基材と接する液柱を作製する、
ことを特徴とする液柱分離デバイス。 - 請求項1に記載の液柱分離デバイスあって、
前記第1基材の前記親水性領域は前記疎水性領域より前記第2基材との距離が近い、
ことを特徴とする液柱分離デバイス。 - 請求項1に記載の液柱分離デバイスあって、
前記第1基材の、任意の隣り合う前記親水性領域の間に、前記第1基材と前記第2基材と連結する柱状物がある、
ことを特徴とする液柱分離デバイス。 - 請求項1に記載の液柱分離デバイスあって、
前記第1基材は、前記親水性領域内に配置された複数の独立電極を有する、
ことを特徴とする液柱分離デバイス。 - 請求項1に記載の液柱分離デバイスあって、
前記第2基材は、前記第1基材の前記親水性領域との対向位置に、親水性領域を持つ、
ことを特徴とする液柱分離デバイス。 - 請求項1に記載の液柱分離デバイスあって、
前記第1基材の前記親水性領域は、シリコン酸化物、またはガラスである、
ことを特徴とする液柱分離デバイス。 - 請求項1に記載の液柱分離デバイスあって、
前記第1基材の前記親水性領域に温調機構を備える、
ことを特徴とする液柱分離デバイス。 - 第1基材と、前記第1基材と所定の間隔を隔てて対向するように配置された第2基材と、前記第1基材と前記第2基材の間に2つ以上の流体を供給する送液手段と、を備え、前記第1基材の前記第2基材と対向する表面は、疎水性の疎水性領域の中に、複数の親水性の親水性領域が配列したパターンを有し、前記親水性領域の代表長さが前記所定の間隔より大きく、前記第1基材と前記第2基材の間に、前記送液手段により2つ以上の非相溶流体を流すことにより、前記第1基材及び前記第2基材と接する液柱を作製し、前記第1基材は前記親水性領域に電気的に独立した第1電極を有し、前記第2基材は前記液柱に接するナノポアを有するメンブレンを有する液柱分離デバイスと、
前記メンブレンに接する電解質溶液を含むチャンバと、
前記チャンバに接する第2電極と、
前記第2電極に接続される測定部と、
前記測定部の測定結果に従い、両電極に印加する電圧を制御する制御部と
を備え、
前記液柱には、生体分子が導入され、前記ナノポアを通過させ、両電極間に流れるイオン電流の時間変化を計測することで、前記生体分子 の通過を検出し、前記生体分子の構造的な特徴を解析する、
ことを特徴とする液柱分離システム。 - 請求項8に記載の液柱分離システムあって、
前記第1基材の前記親水性領域は前記疎水性領域より前記第2基材との距離が近い、
ことを特徴とする液柱分離システム。 - 請求項8に記載の液柱分離システムあって、
前記第1基材の、任意の隣り合う前記親水性領域の間に、前記第1基材と前記第2基材と連結する柱状物がある、
ことを特徴とする液柱分離システム。 - 液柱分離方法であって、
第1基材と、前記第1基材と所定の間隔を隔てて対向するように配置された第2基材と、前記第1基材と前記第2基材の間に2つ以上の流体を供給する送液手段と、を備え、前記第1基材の前記第2基材と対向する表面は、疎水性の疎水性領域の中に、複数の親水性の親水性領域が配列したパターンを有し、前記親水性領域の代表長さが前記所定の間隔より大きい構成の液柱分離デバイスの前記第1基材と前記第2基材の間に、前記送液手段により2つ以上の非相溶流体を流すことにより、前記第1基材及び前記第2基材と接する液柱を作製する、
ことを特徴とする液柱分離方法。 - 請求項11に記載の液柱分離方法あって、
前記第1基材の前記親水性領域は前記疎水性領域より前記第2基材との距離が近い、
ことを特徴とする液柱分離方法。
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