WO2022174512A1 - 一种避光检测系统及其实施方法 - Google Patents
一种避光检测系统及其实施方法 Download PDFInfo
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- WO2022174512A1 WO2022174512A1 PCT/CN2021/087664 CN2021087664W WO2022174512A1 WO 2022174512 A1 WO2022174512 A1 WO 2022174512A1 CN 2021087664 W CN2021087664 W CN 2021087664W WO 2022174512 A1 WO2022174512 A1 WO 2022174512A1
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- 238000001514 detection method Methods 0.000 title claims abstract description 130
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 127
- 238000006243 chemical reaction Methods 0.000 claims abstract description 119
- 230000007246 mechanism Effects 0.000 claims abstract description 52
- 230000033001 locomotion Effects 0.000 claims description 27
- 238000009434 installation Methods 0.000 claims description 21
- 239000000376 reactant Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 6
- 230000000903 blocking effect Effects 0.000 abstract description 4
- 230000009471 action Effects 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 230000010354 integration Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000011229 interlayer Substances 0.000 description 1
- 230000001795 light effect Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Definitions
- the invention relates to the technical field of light-shielding detection by a chemiluminescence immunoanalyzer, in particular to a light-shielding detection system and an implementation method thereof.
- the photodetection link mainly adopts the method of transferring the reaction tube loaded with the detected object to the photodetection component for photon reading.
- This design method can be specially designed to protect the light detection part from light.
- the reaction tube is placed in the light detection part, and after mechanical sealing, the light detection can obtain reliable photon readings.
- reaction tube During the reaction of the reagents, it is necessary to transfer substances in the reaction tube, and the reaction tube will be exposed to the external space; during the light detection period, the reaction tube is transferred to the light detection part to achieve the function of blocking ambient light.
- publication number: CN110133314A patent name: automatic chemiluminescence immunoassay analyzer, the disclosed content.
- the environment in which the reaction and photodetection are located is different, and relatively complex reaction tube/cup transfer design and control are required, and the volume and complexity of the instrument cannot be reduced from the reaction transfer to the detection link.
- the technical problem to be solved by the present invention is to provide a light-proof detection system and an implementation method thereof, which unify the reaction and detection environment, ensure extremely low ambient light in the light detection link, and will not affect the light detection.
- a light-shielding detection system includes an outer light-shielding unit, an inner light-shielding unit, a mobile light-shielding unit, a reagent reaction unit, and a light detection unit;
- the outer light-shielding unit includes a base plate, an outer cover installed on the base plate and a warehouse opening is arranged on the side, and a warehouse door installed in cooperation with the warehouse opening; the inner light-shielding unit, the reagent reaction unit, and the light detection unit are installed on the base plate. and located in the outer cover; the top of the outer cover is provided with a reaction inlet and outlet;
- the mobile light-shielding unit is installed at the reaction inlet and outlet;
- the reagent reaction unit is connected with the inner side of the warehouse door, and the reagent reaction unit makes a linear movement across the entrance or exit of the warehouse;
- the inner light shielding unit is installed on the side of the bottom plate away from the bin opening, and is slidingly matched with the light detection unit.
- the reagent strip is loaded on the reagent reaction unit; the compartment door is closed electrically or manually, so that it is buckled on the cover compartment mouth, and it is closed.
- the reagent reaction unit can pass the reaction inlet and outlet to allow substances or mechanisms to enter and exit the interior of the housing; at the end of the reagent reaction, move the light-shielding unit to seal the reaction inlet and outlet to block external ambient light;
- the detection object performs photon reading to complete the detection process.
- the present invention is based on the external light shielding unit, which blocks most of the external ambient light; the internal light shielding unit blocks the ambient light generated inside the light shielding detection system; the present invention has a simple structure, high integration, and does not require complex control design. Flexible adjustment.
- the light detection unit in order to realize the photon reading of several fixed position detection objects or several movable detection objects; the light detection unit is slidably installed on the bottom plate, and its sliding direction is the same as the movement direction of the reagent reaction unit
- the light detection unit includes a photon counter, a photon counter motion mechanism for controlling the photon counter to slide on the base plate, and a photon counter diaphragm installed on the photosensitive window of the photon counter and slidingly matched with the inner light-shielding unit.
- an inner light shielding unit in order to protect the photon counter from the influence of ambient light during the light detection period, so that the detection result is inaccurate or even damaged, an inner light shielding unit is provided; the inner light shielding unit includes an inner light shielding unit installed on the The photon counter light shielding mechanism and the hidden photoelectric switch trigger mechanism on the side of the reagent reaction unit away from the warehouse mouth; the photon counter light shielding mechanism is slidingly matched with the photon counter aperture.
- a groove is provided on the side of the photon counter light shielding mechanism close to the light detection unit, and the photon counter aperture is close to the photon counter.
- One side of the groove is provided with an annular boss structure, and the photon counter aperture is slidingly matched with the groove.
- a photoelectric switch trigger rod is provided on the side of the hidden photoelectric switch trigger mechanism close to the light detection unit; the photodetection unit is provided with a photoelectric switch trigger block used in conjunction with the photoelectric switch trigger rod .
- the photoelectric switch trigger rod and the photoelectric switch trigger block cooperate with each other to block the light emitted by the photoelectric switch whose positioning/resetting function is performed by the photon counter motion mechanism in the cover, so that the photon counter will not be affected by ambient light.
- the moving light shielding unit in order to enable the opening or closing of the reaction inlet and outlet; includes a light shielding plate fixing member installed on the housing, slidably mounted on the light shielding plate fixing member and used for opening or closing detection An open shading plate; the shading fixing piece is slidingly and sealingly connected with the shading plate.
- a side of the light shielding plate away from the U-shaped structure is provided with a light shielding plate stopper.
- the outer light-shielding unit further includes a warehouse door corresponding to the warehouse opening, and the warehouse door is connected to one end of the reagent reaction unit close to the warehouse opening;
- the reagent reaction unit is a reagent strip loading device that is slidably installed in the outer light-shielding unit and connected to the warehouse door, and the reagent strip loading device has temperature control capability;
- a reagent reaction control device is also installed on the outer side of the housing.
- the reagent strip loading device in order to realize the loading of reagent strips, includes two sets of loading and installation blocks arranged in parallel and one end connected to the compartment door, and several reagent strips are arranged on the loading and installation blocks A hole position; a plurality of the reagent strip hole positions are arranged along the sliding direction of the loading and installation block.
- an implementation method of a light-proof detection system specifically includes the following steps:
- Resetting the relevant parts of the instrument including the resetting of the relevant parts of the light-proof detection system.
- Carry out the test link use the reagent reaction control device to transfer the reactant/reagent to the next reaction/detection position, and control the temperature at the hole position of the reagent strip. After the set time is reached, the reagent and the sample can be fully reacted;
- the present invention is based on the external light-shielding unit to block most of the external ambient light; the internal light-shielding unit blocks the ambient light generated inside the light-shielding detection system; in the middle-stage reagent reaction process of the detection, the reagent reaction unit is used to allow substances or mechanisms to enter and exit
- the light-proof detection system promotes the reagent reaction process; at the end of the reagent reaction, the light-proof detection system is closed by moving the light-proof unit to block the external ambient light; at the end of the detection, the light detection unit performs photon reading on the detected object to complete the detection process;
- the system is simple in structure and high in integration, does not require complicated control design, and can be flexibly adjusted in the implementation method.
- Fig. 1 is the structural representation of the present invention
- Fig. 2 is the positional relationship diagram of the bottom plate and the outer cover in the middle and outer light shielding unit of the present invention
- FIG. 3 is a schematic diagram of other components installed on the bottom plate of the present invention.
- Fig. 4 shows the structure schematic diagram of photon counter light shielding mechanism, photon counter, photon counter motion mechanism, photon counter diaphragm in the present invention
- FIG. 5 is a schematic cross-sectional view of the correlation of the photon counter light-shielding mechanism, the photon counter, and the photon counter aperture in the present invention
- FIG. 6 is a schematic diagram showing the correlation between the hidden photoelectric switch trigger mechanism and the photoelectric switch trigger block in the present invention
- Fig. 7 shows the structural representation of the mobile light shielding unit in the present invention
- FIG. 8 is a schematic diagram of the structural relationship between the reagent reaction control device and the outer cover in the present invention.
- references in this application to "first,” “second,” and similar words do not denote any order, quantity, or importance, but are merely used to distinguish the different components. Likewise, words such as “a” or “an” do not denote a quantitative limitation, but rather denote the presence of at least one. Words like “connected” or “connected” are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
- a number of locating posts refers to one or more locating posts.
- a light-proof detection system comprising an outer light-proof unit, an inner light-proof unit, a mobile light-proof unit, a reagent reaction unit, and a light detection unit;
- the outer light-shielding unit includes a base plate 11, an outer cover 12 installed on the base plate 11 and provided with a warehouse opening 121 on the side, and a warehouse door 13 installed in conjunction with the warehouse opening 121; the inner light-shielding unit, the reagent reaction unit, and The light detection unit is installed on the bottom plate 11 and located in the outer cover 12; the top of the outer cover 12 is provided with a reaction inlet and outlet 122;
- the mobile light shielding unit is installed at the reaction inlet and outlet 122;
- the reagent reaction unit is connected with the inner side of the warehouse door 13 and performs a linear movement across the entrance or exit of the warehouse opening 121;
- the inner light shielding unit is installed on the side of the bottom plate 11 away from the bin opening 121 and slidingly cooperates with the light detection unit.
- the outer cover 12 is in the shape of a box, and forms a light-shielding detection system installation cavity with the bottom plate 11, and the inner light-shielding unit, the mobile light-shielding unit, the reagent reaction unit, and the light detection unit are installed in the inner cavity;
- the bottom of the outer cover 12 is installed on the bottom plate 11, the warehouse opening 121 is arranged on its side, and the reaction inlet and outlet 122 is arranged on its top surface;
- the outer cover 12 has a square structure.
- the bottom plate 11 is provided with an installation groove 111
- the bottom of the reagent reaction unit is provided with a slide rail matched with the installation groove 111 ;
- the external light-proof unit is the main barrier for installing the platform that carries all the components of the light-proof detection system and blocking the external ambient light from entering the light-proof detection system.
- the inner light shielding unit is a device component that blocks the self-luminescence inside the cavity of the light shielding detection system and enhances the light shielding ability of the photon counter 51 .
- the reagent reaction unit supports the conditions required during the reagent reaction process.
- the light detection unit performs photon reading of the detected object during light detection.
- the door 13 is connected with the reagent reaction unit, and when the reagent strip 6 needs to be loaded on the reagent reaction unit, the door 13 is controlled to move away from the outside of the warehouse mouth 121. Since the reagent reaction unit and the bottom plate 11 are in sliding fit, Thereby, the reagent reaction unit is driven to move to one side of the warehouse port 121, and after the reagent reaction unit is pulled out, the loading of the reagent strip 6 can be realized;
- the reagent reaction unit and the compartment door 13 form a drawer structure.
- the warehouse door 13 and the warehouse opening 121 fit together;
- rubber, foam or other flexible materials are provided on the side of the warehouse door 13 and the warehouse opening 121 that are close to each other;
- the warehouse door 13 and the warehouse opening 121 are fitted together.
- the warehouse door 13 When in use, the warehouse door 13 is electrically or manually slid outward to drive the reagent reaction unit to move out of the warehouse port 121 , and the reagent strip 6 is loaded on the reagent reaction unit; mouth 121 and close it.
- the reagent reaction unit can pass the reaction inlet and outlet 122 to allow substances or mechanisms to enter and exit the interior of the housing 12; before the light detection, move the light-shielding unit to seal the reaction inlet and outlet 122 to block external ambient light; light detection in the final stage of detection The unit performs photon reading on the detected object to complete the detection process.
- the reagent strip 6 described here can be a sample carrier or a reagent carrier.
- the present invention is based on the external light shielding unit, which blocks most of the external ambient light; the internal light shielding unit blocks the ambient light generated inside the light shielding detection system; the present invention has a simple structure, high integration, and does not require complex control design. Flexible adjustment.
- the bottom of the light detection unit is provided with a sliding rail matched with the installation groove 111, and its sliding direction is the same as that of the mounting groove 111.
- the movement direction of the reagent reaction unit is the same;
- the light detection unit includes a photon counter 51, a photon counter motion mechanism 52 for controlling the photon counter 51 to slide on the bottom plate 11, and a photon counter 51 installed on the photosensitive window of the photon counter 51. The unit slides to fit the photon counter aperture 53.
- the photon counter 51 is fixed on the photon counter motion mechanism 52, and the photon counter motion mechanism 52 is used to control the photon counter 51 to slide along the long direction of the installation slot 111, and bring the photon counter 51 to the reset position and each light detection position.
- the photon counter diaphragm 53 is fixed on the photosensitive window of the photon counter 51 to ensure that the stray light enters the photosensitive window of the photon counter 51 as little as possible during light detection, so that the photon reading is reliable; the photon counter movement mechanism 52 runs to the reset position, and cooperates with The inner light shielding unit greatly reduces the ambient light entering the photosensitive window of the photon counter 51 .
- the detection result will be inaccurate or even damaged;
- the photon counter light-shielding mechanism 21 and the hidden photoelectric switch trigger mechanism 22 on the side of the reagent reaction unit away from the bin opening 121 are slidably matched with the photon counter aperture 53 .
- the photon counter light-shielding mechanism 21 is not limited to the shutter structure installed on the photosensitive window of the photon counter 51 controlled by an electric method, and may also be a mechanical light-shielding bin structure when the photon counter 51 moves to a fixed position.
- the former can be controlled to avoid light anytime and anywhere; the latter can be used in the movement mode of the photon counter 51, which can realize a fixed position to avoid light, and the design is relatively simple.
- the photon counter light shielding mechanism 21 in order to effectively realize the sliding cooperation between the photon counter light shielding mechanism 21 and the photon counter 51 ; the photon counter light shielding mechanism 21 is close to the side of the light detection unit.
- a groove is provided, the photon counter diaphragm 53 is provided with a circular boss structure on one side close to the groove, and the photon counter diaphragm 53 is slidably matched with the groove.
- the longitudinal axis of the groove is arranged in parallel with the longitudinal axis of the installation groove 111 .
- the long direction of the slide rail is consistent with the sliding direction of the reagent reaction unit on the bottom plate 11 , and the light detection unit can be detected at different positions by sliding fit.
- a photon counter 51 is mounted on the photon counter motion mechanism 52, and when it runs to the reset position, the photon counter diaphragm 53 installed on the photon counter 51 photosensitive window enters the photon counter light-proof mechanism 21 interior;
- the side of the photon counter diaphragm 53 close to the groove is provided with an annular boss, and the light can only enter the photosensitive window of the photon counter 51 through the center of the annular boss; when the photon counter motion mechanism 52 completes the reset , the annular boss enters the groove, and the distance between the two is very small.
- the two materials are treated with blackening to block light from entering the photosensitive window of the photon counter 51 , so as to achieve the purpose of avoiding light of the photon counter 51 .
- a shutter is set on the photon counter diaphragm 53, and the shutter is opened when light detection is required; the shutter is closed at other times, so that the photon counter 51 can be protected from light at any time and any position.
- a photoelectric switch trigger lever 221 is provided on the side of the hidden photoelectric switch trigger mechanism 22 close to the light detection unit; The photoelectric switch used in conjunction with the rod 221 triggers the block 521 .
- the photoelectric switch trigger block 521 is installed on the side of the photon counter motion mechanism close to the photoelectric switch trigger rod 221 .
- the photoelectric switch trigger lever 221 cooperates with the photoelectric switch trigger block 521 to block the light emitted by the photoelectric switch, which plays the role of positioning/resetting the photon counter motion mechanism 52 in the outer cover 12 , so that it will not cause ambient light effects to the photon counter 51 . .
- the photon counter movement mechanism 52 runs to the reset position, which is determined by the hidden photoelectric switch trigger mechanism 22; when the photon counter movement mechanism 52 moves to the reset direction, the photoelectric switch trigger block 521 contacts the photoelectric switch trigger rod 221, and the latter is moved. Press into the hidden photoelectric switch trigger mechanism 22 .
- the hidden photoelectric switch trigger mechanism 22 is provided with a reset photoelectric switch, and the photoelectric switch trigger rod 221 enters the reset switch photosensitive area to generate a reset photoelectric signal, and the photon counter motion mechanism 52 stops moving to realize the photon counter motion mechanism 52 reset action.
- the self-luminescence generated by the reset photoelectric switch is blocked by the internal structure and the spacer/block in the hidden photoelectric switch trigger mechanism 22, and cannot be emitted to the inner cavity of the light-proof detection system.
- the photoelectric switch trigger rod 221 pops up automatically, and the reset photoelectric signal disappears.
- the photon counter movement mechanism 52 is mainly used to control the movement of the light detection unit along the long direction of the installation slot 111 , and may be a screw drive device or a linear push rod control.
- the moving light shielding unit in order to open or close the reaction inlet and outlet 122; as shown in FIG. 1 and FIG. 7 , the moving light shielding unit includes a light shielding plate fixing member 31 installed on the housing 12, and a shading plate fixing member 31 installed on the outer cover 12.
- the shading plate 32 in order to realize the connection between the light-shielding fixing member and the light-shielding plate 32 and avoid light leakage at the connection; Channel 311 ; the shading plate 32 is provided with a shading plate boss 321 which is installed in cooperation with the shading plate channel 311 .
- the shading plate channel 311 has a U-shaped structure and is provided with an opening.
- the shading plate fixing member 31 is connected with the outer cover 12 by bolts.
- flexible materials such as rubber pads and sealing strips can be placed between the shading plate fixing member 31 and the cover 12 to isolate external ambient light from entering the light-proof detection system through the gap between the shading plate fixing member 31 and the outer cover 12 .
- the side of the light shielding plate 32 away from the U-shaped structure is provided with a light shielding plate stopper 322 .
- the shading plate 32 moves from the opening side of the U-shaped structure to the bottom side thereof, thereby realizing the closing of the reaction inlet and outlet 122, and opening on the contrary;
- the recessed depth and width of the light-shielding plate channel 311 match the light-shielding plate bosses 321;
- the plate channel 311 is exactly matched with the light-shielding plate bosses 321 on the three sides; the open end of the light-shielding plate fixing member 31 is attached to the light-shielding plate stopper 322 . At this time, the light cannot enter the housing 12 through the cavity of the shading plate fixing member 31 .
- an installation and positioning hole is provided on the light-shielding plate 32; a light-shielding plate control arm is installed on the installation and positioning hole to shield and expose the light-shielding plate fixing member 31 along the light-shielding plate channel 311, so that the reagent During the reaction, substances or mechanisms can enter and exit the light-proof detection system through the reaction inlet 122 to advance the reagent reaction process; at the end of the reagent reaction, the light-shielding plate 32 is controlled to close the light-proof detection system to achieve the purpose of blocking external ambient light.
- the shading plate control arm is disposed on the top surface of the shading plate 32 .
- a wear-resistant material is used as an interlayer between the light-shielding plate fixing member 31 and the light-shielding plate boss 321, which is beneficial to reduce the friction between the two and improve the sliding smoothness.
- the reagent reaction unit is a reagent strip loading device 41 slidably installed on the bottom plate 11 and connected to the compartment door 13 ;
- the reagent reaction unit is mainly used for loading reagent strips 6 and controlling the temperature during the reaction;
- the reagent strip loading device 41 is provided with a reagent strip temperature control component, which can independently control the temperature of the two reagent strip loading devices 41, thereby indirectly controlling the temperature of the liquid inside the reagent strip 6, so as to keep it within the reagent system regulations. within the temperature range; to meet the needs of the reagent reaction process.
- a reagent reaction control device 7 is also included.
- the reagent reaction control device 7 is arranged on the top of the outer cover 12 and can realize the function of extracting the reacted/reactant to the next reaction/detection position.
- the inner cavity of the light-shielding detection system is communicated with the outside world, and the reagent reaction control device 7 enters the housing 12 through the reaction inlet 122, thereby realizing various functions, such as moving the reagent to the next reaction/detection position, to Adapt to the requirements of the reagent system and flexibly realize the test process.
- the reagent reaction control device 7 exits the interior of the housing 12, and the light shielding plate 32 shields the reaction inlet and outlet 122, and then photon reading can be performed.
- the reagent reaction control device 7 is a magnetic suction device or a liquid suction needle; when it is a magnetic suction device, by adsorbing the magnetic beads of the reactant, the magnetic suction device is moved to the next reaction position to carry out the next reaction.
- the magnetic suction device can be a magnetic rod.
- the reactant is sucked by the pipetting needle, and then the pipetting needle is moved to the next reaction position to carry out the next reaction.
- the reagent strip loading device 41 in order to realize the loading of the reagent strips 6 , includes two sets of loading and installation blocks arranged in parallel and connected to the compartment door 13 at one end.
- the mounting block is provided with several 6-hole positions for reagent strips, which are suitable for application scenarios of loading one or more reagent strips 6; several of the reagent strips 6-hole positions are arranged along the sliding direction of the loading and mounting block.
- the first and last ends of the reagent strip 6 are respectively loaded on two sets of loading and installation blocks, and the two sets of loading and installation blocks provide stable reaction and detection loading positions for the reagent strip 6;
- a hole or a groove is opened in the middle of the loading block at the end of the loading reagent strip 6 to provide a detection window for light detection.
- a special central opening may not be provided.
- An implementation method of a light-proof detection system specifically comprising the following steps:
- Resetting the relevant parts of the instrument including the resetting of the relevant parts of the light-proof detection system.
- Carry out the test link use the reagent reaction control device 7 to transfer the reactant/reagent to the next reaction/detection position, and perform temperature control at the 6-well position of the reagent strip. After the set time is reached, the reagent and the sample are fully reacted;
- the mobile light shielding unit is turned off, and the photon counter 51 reads the photons of the detected object. Specifically:
- the reagent reaction control device 7 exits the housing 12 and closes the reaction inlet and outlet 122; the photon counter movement mechanism 52 moves to the first photodetection position, and the photon counter 51 performs photon reading; as required, the next photon detection position moves and photons Reads until photon readings at all locations are complete.
- the present invention unifies the reaction and detection environments, ensures that the ambient light is extremely low in the light detection link, and does not affect the light detection. Therefore, the instrument can be greatly simplified in structure and design during the detection process, and the volume and weight of the instrument can be fundamentally compressed.
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Abstract
一种避光检测系统，包括外避光单元、内避光单元、试剂反应单元、光检测单元以及移动避光单元。避光检测系统的实施方法是利用各单元进行检测动作；检测动作包括检测前期的准备工作、检测中期试剂反应过程，和检测末期对被检测物进行光子读数。以外避光单元为基础，阻隔大部分外部环境光；以内避光单元阻隔避光检测系统内部产生的环境光；在检测中期试剂反应过程中，应用试剂反应单元允许物质或机构进出避光检测系统，推进试剂反应过程；试剂反应末期通过移动避光单元来闭合避光检测系统，实现外部环境光的阻隔；检测末期光检测单元对被检测物进行光子读数，完成检测过程。
Description
本发明涉及化学发光免疫分析仪避光检测技术领域，特别涉及一种避光检测系统及其实施方法。
目前的测试中光检测环节，主要采用将装载了被检测物的反应管转移到光检测部件中进行光子读数的方法。
这种设计方式可以对光检测部件进行专门的避光设计，反应管放置进光检测部件中，经过机械密闭，使光检测得到可靠的光子读数。
在试剂反应期间，需要在反应管中进行物质的转移，反应管会暴露在外部空间中；在光检测期间，再将反应管转移到光检测部件中，实现阻隔环境光的作用。如：公开号：CN110133314A，专利名称为：全自动化学发光免疫分析仪，所公开的内容。反应和光检测所处的环境不同，需要进行相对复杂的反应管/杯转移设计和控制，仪器体积和复杂程度不能在反应转移到检测环节得到降低。
发明内容
本发明所要解决的技术问题是，提供一种避光检测系统及其实施方法，将反应和检测环境统一，确保了在光检测环节极低环境光，对光检测不会造成影响。
本发明解决技术问题所采用的解决方案是：
一方面，一种避光检测系统，包括外避光单元、内避光单元、移动避光单元、试剂反应单元、以及光检测单元；
所述外避光单元包括底板、安装在底板上且侧面设置有仓口的外罩、以及与仓口配合安装的仓门；所述内避光单元、试剂反应单元、以及光检测单元安装在底板上且位于外罩内；所述外罩的顶部设置有反应进出口；
所述移动避光单元安装在反应进出口；
所述试剂反应单元与仓门的内侧面连接，试剂反应单元做跨进或跨出仓口的直线运动；
所述内避光单元安装在底板远离仓口的一侧且与光检测单元滑动配合。
试剂条装载在试剂反应单元上；仓门电动或手动关闭，使之扣在外罩仓口上，并将其封闭。在检测中期试剂反应过程中，试剂反应单元通过反应进出口，可以实现物质或机构进出外罩内部；试剂反应末期移动避光单元封闭反应进出口，实现阻隔外部环境光；检测末期光检测单元对被检测物进行光子读数，完成检测过程。
本发明以外避光单元为基础，阻隔大部分外部环境光；以内部避光单元阻隔避光检测系统内部产生的环境光；本发明结构简单、集成度高，不需要复杂的控制设计，实施方法可灵活调整。
在一些可能的实施方式中，为了实现若干个固定位置检测对象或若干个可运动检测对象的的光子读数；所述光检测单元滑动安装在底板上，其滑动方向与试剂反应单元的运动方向相同；所述光检测单元包括光子计数器、用于控制光子计数器在底板上滑动的光子计数器运动机构、安装在光子计数器的感光窗口上且与内避光单元滑动配合的光子计数器光阑。
在一些可能的实施方式中，为了保护光子计数器不在光检测期间，免受环境光影响，使其检测结果不准确甚至使其损坏，设置有内避光单元；所述内避 光单元包括安装在试剂反应单元远离仓口一侧的光子计数器避光机构和隐藏式光电开关触发机构；所述光子计数器避光机构与光子计数器光阑滑动配合。
在一些可能的实施方式中，为了有效的实现光子计数器避光机构与光子计数器的滑动配合；所述光子计数器避光机构靠近光检测单元的一侧设置有凹槽，所述光子计数器光阑靠近凹槽的一侧设置有圆环状凸台结构，所述光子计数器光阑与凹槽滑动配合。
在一些可能的实施方式中，所述隐藏式光电开关触发机构靠近光检测单元的一侧设置有光电开关触发杆；所述光检测单元上设置有与光电开关触发杆配合使用的光电开关触发块。
光电开关触发杆与光电开关触发块相互配合，对外罩内的光子计数器运动机构起定位/复位作用的光电开关发射的光线进行阻隔，使其不会对光子计数器造成环境光影响。
在一些可能的实施方式中，为了使得反应进出口的打开或关闭；所述移动避光单元包括安装在外罩上的遮光板固定件、滑动安装在遮光板固定件上且用于打开或关闭检测开口的遮光板；所述遮光固定件与遮光板滑动密封连接。
在一些可能的实施方式中，为了实现遮光固定件与遮光板的连接，避免连接处出现漏光；所述遮光板固定件呈U型结构，其内侧设置有遮光板槽道；所述遮光板设置有与开口配合安装的遮光板凸台。
在一些可能的实施方式中，所述遮光板远离U型结构的一侧设置有遮光板挡头。
在一些可能的实施方式中，所述外避光单元还包括与仓口对应设置的仓门，所述仓门与试剂反应单元靠近仓口一端连接；
所述试剂反应单元为滑动安装在外避光单元内且与仓门连接的试剂条装载装置，试剂条装载装置具有温度控制能力；
在所述外罩的外侧面上还安装有试剂反应控制装置。
在一些可能的实施方式中，为了实现对于试剂条的装载，所述试剂条装载装置包括两组平行设置且一端与仓门连接的装载安装块，所述装载安装块上设置有若干个试剂条孔位；若干个所述试剂条孔位沿装载安装块的滑动方向设置。
另一方面，一种避光检测系统的实施方法，具体包括以下步骤：
仪器相关部件复位；包括避光检测系统相关部件复位。
进行测试前准备：更换测试使用的其他一次性耗材。将试剂条放置在试剂条装载装置上，将样本放入仪器指定位置，关闭仓门和封闭反应进出口；
进行测试环节：使用试剂反应控制装置转移被反应物/试剂到下一个反应/检测位置，在试剂条孔位进行温度控制，达到设定时间后，实现试剂和样本充分的反应；
反应结束后，关闭移动避光单元，光子计数器对被检测物进行光子读数；
检测结束，仪器相关部件复位。
与现有技术相比，本发明的有益效果：
本发明以外避光单元为基础，阻隔大部分外部环境光；以内部避光单元阻隔避光检测系统内部产生的环境光；在检测中期试剂反应过程中，应用试剂反应单元，允许物质或机构进出避光检测系统，推进试剂反应过程；试剂反应末期通过移动避光单元来闭合避光检测系统，实现阻隔外部环境光；检测末期光检测单元对被检测物进行光子读数，完成检测过程；
本系统结构简单、集成度高，不需要复杂的控制设计，实施方法可灵活调整。
图1为本发明的结构示意图；
图2为本发明中外避光单元中底板和外罩的位置关系图；
图3示为本发明中底板上安装的其他部件示意图；
图4示为本发明中光子计数器避光机构、光子计数器、光子计数器运动机构、光子计数器光阑的结构示意图；
图5示为本发明中光子计数器避光机构、光子计数器、光子计数器光阑相关性剖面示意图；
图6示为本发明中隐藏式光电开关触发机构与光电开关触发块的相关性示意图；
图7示为本发明中移动避光单元的结构示意图；
图8为本发明中试剂反应控制装置与外罩的结构关系示意图；
其中：11、底板；111、安装槽；12、外罩；121、仓口；122、反应进出口；13、仓门；21、光子计数器避光机构；22、隐藏式光电开关触发机构；221、光电开关触发杆；31、遮光板固定件；311、遮光板槽道；32、遮光板；321、遮光板凸台；322、遮光板挡头；41、试剂条装载装置；51、光子计数器；52、光子计数器运动机构；521、光电开关触发块；53、光子计数器光阑；6、试剂条；7、试剂反应控制装置。
下面将结合本申请实施例中的附图，对本申请实施例中的技术方案进行清 楚、完整地描述，显然，所描述的实施例是本申请一部分实施例，而不是全部的实施例。基于本申请中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本申请保护的范围。
本申请所提及的"第一"、"第二"以及类似的词语并不表示任何顺序、数量或者重要性，而只是用来区分不同的组成部分。同样，"一个"或者"一"等类似词语也不表示数量限制，而是表示存在至少一个。"连接"或者"相连"等类似的词语并非限定于物理的或者机械的连接，而是可以包括电性的连接，不管是直接的还是间接的。
在本申请实施中，“和/或”描述关联对象的关联关系，表示可以存在三种关系，例如，A和/或B，可以表示：单独存在A，同时存在A和B，单独存在B这三种情况。
在本申请实施例的描述中，除非另有说明，“若干个”的含义是指一个或一个以上。例如，若个定位柱是指一个或一个以上的定位柱。
下面结合附图和实施例对本发明做进一步说明。
本发明通过下述技术方案实现，如图1-图8所示，
一种避光检测系统，包括外避光单元、内避光单元、移动避光单元、试剂反应单元、以及光检测单元；
所述外避光单元包括底板11、安装在底板11上且侧面设置有仓口121的外罩12、以及与仓口121配合安装的仓门13；所述内避光单元、试剂反应单元、以及光检测单元安装在底板11上且位于外罩12内；所述外罩12的顶部设置有反应进出口122；
所述移动避光单元安装在反应进出口122；
所述试剂反应单元与仓门13的内侧面连接且做跨进或跨出仓口121的直线运动；
所述内避光单元安装在底板11远离仓口121的一侧且与光检测单元滑动配合。
优选的，外罩12呈箱体状，与底板11形成一个避光检测系统安装内腔，内避光单元、移动避光单元、试剂反应单元、以及光检测单元安装在内腔内；
外罩12的底部安装在底板11上，仓口121设置在其侧面，反应进出口122设置在其顶面；
优选的，如图1、图2所示，外罩12呈方形体结构。
优选的，底板11上设置有安装槽111，所述试剂反应单元的底部设置有与安装槽111配合的滑轨；所述安装槽111的长方向与仓门13的侧面相互垂直。
外避光单元为安装承载避光检测系统所有零部件的平台和阻隔外部环境光进入避光检测系统的主要屏障。
内避光单元为阻隔避光检测系统腔体内部自发光和加强光子计数器51避光能力的设备组件。
移动避光单元在进行光检测前，封闭检测运行进出口。
试剂反应单元支持试剂反应过程中所需要的条件。
光检测单元在光检测期间对被检测物进行光子读数。
本发明中，将仓门13与试剂反应单元连接，需要将试剂条6装载在试剂反应单元上时，控制仓门13向远离仓口121外侧运动，由于试剂反应单元与底板11为滑动配合，从而将带动试剂反应单元向仓口121的一侧运动，试剂反应单 元被拉出后，即可实现试剂条6的装载；
试剂反应单元与仓门13形成一个抽屉结构。
优选的，仓门13与仓口121在关闭时，仓门13与仓口121贴合；
为了使得在关闭时两者贴合更加紧密，在所述仓门13和仓口121相互靠近的一侧设置有橡胶、泡棉或其他柔性材料；
为了有效的阻隔光线从仓门13与仓口121的连接处进入避光检测系统内部；仓门13与仓口121采用嵌合的方式。
使用时，仓门13电动或手动向外滑动，带动试剂反应单元使之移出仓口121，试剂条6装载在试剂反应单元上；仓门13电动或手动关闭，使之扣在外罩12的仓口121上，并将其封闭。在检测中期试剂反应过程中，试剂反应单元通过反应进出口122，可以实现物质或机构进出外罩12内部；光检测前移动避光单元封闭反应进出口122，实现阻隔外部环境光；检测末期光检测单元对被检测物进行光子读数，完成检测过程。
这里所描述的试剂条6可以为样本承载体，也可以为试剂承载体。
本发明以外避光单元为基础，阻隔大部分外部环境光；以内部避光单元阻隔避光检测系统内部产生的环境光；本发明结构简单、集成度高，不需要复杂的控制设计，实施方法可灵活调整。
在一些可能的实施方式中，为了实现若干个固定位置检测对象或若干个可运动检测对象的的光子读数；所述光检测单元的底部设置有与安装槽111配合的滑轨，其滑动方向与试剂反应单元的运动方向相同；所述光检测单元包括光子计数器51、用于控制光子计数器51在底板11上滑动的光子计数器运动机构52、安装在光子计数器51的感光窗口上且与内避光单元滑动配合的光子计数器 光阑53。
光子计数器51固定在光子计数器运动机构52上，光子计数器运动机构52用于控制光子计数器51沿安装槽111的长方向滑动，将光子计数器51带到复位位置和各个光检测位置。
光子计数器光阑53固定在光子计数器51的感光窗口上，确保在进行光检测时杂散光尽量少的进入光子计数器51感光窗口，使光子读数可靠；光子计数器运动机构52运行到复位位置上，配合内避光单元，极大的减弱光子计数器51感光窗口进入的环境光。
在一些可能的实施方式中，如图3所示，为了保护光子计数器51不在光检测期间，免受环境光影响，使其检测结果不准确甚至使其损坏；所述内避光单元包括安装在试剂反应单元远离仓口121一侧的光子计数器避光机构21和隐藏式光电开关触发机构22；所述光子计数器避光机构21与光子计数器光阑53滑动配合。
光子计数器避光机构21，不限于电动方式控制的安装在光子计数器51感光窗口上的快门结构，也可以是光子计数器51运动到固定位置时的机械避光仓结构。前者能实现随时随地受控避光；后者能在光子计数器51运动方式上使用，可实现固定位置避光，设计相对简单。
在一些可能的实施方式中，如图4、图5所示，为了有效的实现光子计数器避光机构21与光子计数器51的滑动配合；所述光子计数器避光机构21靠近光检测单元的一侧设置有凹槽，所述光子计数器光阑53靠近凹槽的一侧设置有圆环状凸台结构，所述光子计数器光阑53与凹槽滑动配合。
优选的，凹槽的长方向轴线与安装槽111的长方向轴线平行设置。
滑轨的长方向与试剂反应单元在底板11上的滑动方向一致，通过滑动配合可实现光检测单元在不同位置进行检测。
优选的，如图4所示，在光子计数器运动机构52上搭载光子计数器51，其运行到复位位置时，光子计数器51感光窗口上安装的光子计数器光阑53进入光子计数器避光机构21内部；
如图5所示，光子计数器光阑53靠近凹槽的一侧设置有圆环状凸台，光线只能通过环状凸台中心进入光子计数器51感光窗口；当光子计数器运动机构52完成复位后，圆环状凸台进入凹槽内，且两者之间距离很小。两者材料采用发黑处理，阻隔光线进入光子计数器51感光窗口，实现光子计数器51避光目的。
优选的，在光子计数器光阑53上设置快门，在需要进行光检测时候，打开快门；其余时间关闭快门，可以实现光子计数器51在任何时间任何位置避光的要求。
在一些可能的实施方式中，如图6所示，所述隐藏式光电开关触发机构22靠近光检测单元的一侧设置有光电开关触发杆221；所述光检测单元上设置有与光电开关触发杆221配合使用的光电开关触发块521。
光电开关触发块521安装在光子计数器运动机构靠近光电开关触发杆221一侧。
光电开关触发杆221与光电开关触发块521相互配合，对外罩12内的光子计数器运动机构52起定位/复位作用的光电开关发射的光线进行阻隔，使其不会对光子计数器51造成环境光影响。
光子计数器运动机构52运行到复位位置，是由隐藏式光电开关触发机构22决定的；当光子计数器运动机构52向复位方向运动，光电开关触发块521接触 到光电开关触发杆221，并将后者压进隐藏式光电开关触发机构22内部。
隐藏式光电开关触发机构22内部设有复位光电开关，光电开关触发杆221进入复位开关感光区域，产生复位光电信号，光子计数器运动机构52停止运动，实现光子计数器运动机构52复位动作。复位光电开关产生的自发光，在隐藏式光电开关触发机构22内部，被内部结构和隔圈/挡片所阻隔，不能散发到避光检测系统内腔。当光子计数器运动机构52向外运动，光电开关触发杆221自动弹出，复位光电信号消失。
优选的，光子计数器运动机构52主要用于控制光检测单元沿安装槽111的长方向运动，可以为丝杠驱动装置、也可以采用直线推杆控制。
在一些可能的实施方式中，为了使得反应进出口122的打开或关闭；如图1、图7所示，所述移动避光单元包括安装在外罩12上的遮光板固定件31、滑动安装在遮光板固定件31上且用于打开或关闭检测开口的遮光板32；所述遮光固定件31与遮光板32滑动密封连接。
在一些可能的实施方式中，如图7所示，为了实现遮光固定件与遮光板32的连接，避免连接处出现漏光；所述遮光板固定件31呈U型结构，其内侧设置有遮光板槽道311；所述遮光板32设置有与遮光板槽道311配合安装的遮光板凸台321。
遮光板槽道311为U型结构，设置有开口。
遮光板固定件31与外罩12通过螺栓连接。
优选的，遮光板固定件31与外罩12之间可以垫橡胶垫、密封条等柔性材料，以隔绝外部环境光通过遮光板固定件31与外罩12之间间隙进入避光检测系统内部。
优选的，所述遮光板32远离U型结构的一侧设置有遮光板挡头322。
使用时，遮光板32由U型结构的开口一侧向其底部一侧移动，从而实现反应进出口122的关闭，相反则实现打开；
优选的，遮光板槽道311凹陷的深度和宽度与遮光板凸台321相匹配；遮光板凸台321设在遮光板32的三面，当遮光板32插入遮光板固定件31内部到底时，遮光板槽道311与三个面上的遮光板凸台321刚好完全匹配；遮光板固定件31的开口端和遮光板挡头322贴合。此时光线不能通过遮光板固定件31的腔室进入外罩12内。
优选的，在所述遮光板32上设置有安装定位孔；在所述安装定位孔上安装遮光板控制臂，实现对遮光板固定件31沿遮光板槽道311的遮蔽和露出，使在试剂反应期间，可以通过反应进出口122实现物质或机构进出避光检测系统，来推进试剂反应过程；试剂反应末期通过控制遮光板32来闭合避光检测系统，实现阻隔外部环境光目的。
优选的，遮光板控制臂设置在遮光板32的顶面。
优选的，遮光板固定件31与遮光板凸台321之间，采用耐磨材料作为隔层，有利于降低两者之间的摩擦，提高滑动顺滑度。
在一些可能的实施方式中，如图3所示，所述试剂反应单元为滑动安装在底板11上且与仓门13连接的试剂条装载装置41；
试剂反应单元主要用于装载试剂条6，并对反应过程中的温度进行控制；
试剂条6内装载样本和各种试剂；
试剂条装载装置41设有试剂条温度控制组件，可以对两块试剂条装载装置 41块进行独立的温度控制，由此间接对试剂条6内部的液体进行温度控制，使其保持在试剂体系规定的温度范围内；以适应试剂反应过程中的需求。
如图8所示，优选的，还包括试剂反应控制装置7，试剂反应控制装置7设在外罩12的顶部，可以实现提取被反应物/反应物到下一个反应/检测位置的作用。
在试剂反应期间，避光检测系统内腔与外界联通，试剂反应控制装置7通过反应进出口122进入外罩12内，由此可以实现多种功能，如移动试剂到下一个反应/检测位置，以适应试剂体系要求，灵活的实现测试过程。
试剂反应过程结束后，试剂反应控制装置7退出外罩12内部，遮光板32对反应进出口122进行遮蔽，之后可以进行光子读数。
优选的，试剂反应控制装置7为磁吸设备或者吸液针；当其为磁吸设备时，通过吸附反应物的磁珠，再将磁吸设备移动到下一反应位置，进行下一步的反应；磁吸设备可以为磁棒。
当其为吸液针时，通过吸液针吸取反应物，再将吸液针移动到下一反应位置，进行下一步的反应。
在一些可能的实施方式中，如图3所示，为了实现对于试剂条6的装载，所述试剂条装载装置41包括两组平行设置且一端与仓门13连接的装载安装块，所述装载安装块上设置有若干个试剂条6孔位，以适用于装载一个或多个试剂条6的应用场景；若干个所述试剂条6孔位沿装载安装块的滑动方向设置。
试剂条6的首末两端分别装载在两组装载安装块上，两组装载安装块为试剂条6提供稳固的反应和检测装载位；
优选的，装载试剂条6末端的装载安装块中部开孔或开槽，为光检测提供 检测窗口。当然，根据光子计数器51安装方向和位置，可以不设置专门的中部开孔。
一种避光检测系统的实施方法，具体包括以下步骤：
仪器相关部件复位，包括避光检测系统相关部件复位。
进行测试前准备：更换测试使用的其他一次性耗材。将试剂条6放置在试剂条装载装置41上，将样本放入仪器指定位置，关闭仓门13和封闭反应进出口122；具体是指：
拉出或自动弹出仓门13，手动装载试剂条6到试剂条装载装置41上，将样本放入仪器指定位置；关闭仓门13，封闭系统外罩12的仓口121。
进行测试环节：使用试剂反应控制装置7转移被反应物/试剂到下一个反应/检测位置，在试剂条6孔位进行温度控制，达到设定时间后，实现试剂和样本充分的反应；
反应结束后，关闭移动避光单元，光子计数器51对被检测物进行光子读数。具体是指：
试剂反应控制装置7退出外罩12内部，关闭反应进出口122；光子计数器运动机构52运动到第一个光检测位置，光子计数器51进行光子读数；按照要求，进行下一个光检测位置的移动和光子读数，直到完成所有位置的光子读数。
检测结束，仪器相关部件复位。
综上所述，本发明将反应和检测环境统一，确保了在光检测环节极低环境光，对光检测不会造成影响。由此仪器在检测过程中，能得到结构和设计上极大的简化，仪器体积和重量可以得到根本性的压缩。
以上对本申请实施例进行了详细介绍，本申请中应用了具体个例对本申请 的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本申请的方法及其核心思想；同时，对于本领域的一般技术人员，依据本申请的思想，在具体实施方式及应用范围上均会有改变之处，综上所述，本说明书内容不应理解为对本申请的限制。
Claims (10)
- 一种避光检测系统，其特征在于：包括外避光单元、内避光单元、移动避光单元、试剂反应单元、以及光检测单元；所述内避光单元、试剂反应单元、以及光检测单元安装在外避光单元内部；所述外避光单元包括设置有侧面设置有仓口和顶面设置有反应进出口的外罩，所述试剂反应单元滑动安装在外避光单元内且做跨进或跨出仓口的直线运动；所述内避光单元设置于试剂反应单元远离仓口的一侧；所述光检测单元与内避光单元滑动配合且沿试剂反应单元的滑动方向滑动；所述移动避光单元安装在反应进出口上且用于打开或关闭反应进出口。
- 根据权利要求1所述的一种避光检测系统，其特征在于：所述光检测单元包括光子计数器、用于控制光子计数器滑动的光子计数器运动机构、安装在光子计数器的感光窗口上且与内避光单元滑动配合的光子计数器光阑。
- 根据权利要求2所述的一种避光检测系统，其特征在于：所述内避光单元包括安装在试剂反应单元远离仓口一侧的光子计数器避光机构和隐藏式光电开关触发机构；所述光子计数器避光机构与光子计数器光阑滑动配合。
- 根据权利要求3所述的一种避光检测系统，其特征在于：所述光子计数器避光机构靠近光检测单元的一侧设置有凹槽，所述光子计数器光阑靠近凹槽的一侧设置有圆环状凸台结构，所述光子计数器光阑与凹槽滑动配合。
- 根据权利要求3所述的一种避光检测系统，其特征在于：所述隐藏式光电开关触发机构靠近光检测单元的一侧设置有光电开关触发杆；所述光检测单 元上设置有与光电开关触发杆配合使用的光电开关触发块。
- 根据权利要求1所述的一种避光检测系统，其特征在于：所述外避光单元还包括底板，所述外罩安装在底板上；所述移动避光单元包括安装在外罩上的遮光板固定件、滑动安装在遮光板固定件上且用于打开或关闭检测开口的遮光板；所述遮光固定件与遮光板滑动密封连接。
- 根据权利要求6所述的一种避光检测系统，其特征在于：所述遮光板固定件呈U型结构，其内侧设置有遮光板槽道；所述遮光板设置有与开口配合安装的遮光板凸台。
- 根据权利要求1-7任一项所述的一种避光检测系统，其特征在于：所述外避光单元还包括与仓口对应设置的仓门，所述仓门与试剂反应单元靠近仓口一端连接；所述试剂反应单元为滑动安装在外避光单元内且与仓门连接的试剂条装载装置，试剂条装载装置具有温度控制能力；在所述外罩的外侧面上还安装有试剂反应控制装置。
- 根据权利要求8所述的一种避光检测系统，其特征在于：所述试剂条装载装置包括两组平行设置且一端与仓门连接的装载安装块，所述装载安装块上设置有若干个试剂条孔位；若干个所述试剂条孔位沿装载安装块的滑动方向设置。
- 根据权利要求1-9任一项所述的一种避光检测系统的实施方法，其特征在于：具体包括以下步骤：仪器相关部件复位，包括避光检测系统相关部件复位。更换测试使用的其他一次性耗材；将试剂条放置在试剂条装载装置上，将样本放入仪器指定位置，关闭仓门和封闭反应进出口；进行测试环节；使用试剂反应控制装置转移被反应物/试剂到下一个反应/检测位置，在试剂条孔位进行温度控制，达到设定时间后，实现试剂和样本充分的反应；反应结束后，关闭移动避光单元，光子计数器对被检测物进行光子读数；检测结束，仪器相关部件复位，包括避光检测系统相关部件复位。
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223218A (en) * | 1987-04-09 | 1993-06-29 | Kabushiki Kaisha Meidensha | Instrument for quantitative analysis |
| US20150177236A1 (en) * | 2013-03-15 | 2015-06-25 | Gold Standard Diagnostics | Combined chemiluminescence and elisa automated sample reader |
| CN107923924A (zh) * | 2015-08-27 | 2018-04-17 | 株式会社日立高新技术 | 自动分析装置 |
| CN209148550U (zh) * | 2018-11-09 | 2019-07-23 | 广州军区广州总医院 | 一种纸电化学发光芯片用避光检测装置 |
| CN110133314A (zh) * | 2018-02-08 | 2019-08-16 | 成都深迈瑞医疗电子技术研究院有限公司 | 全自动化学发光免疫分析仪 |
| CN209311331U (zh) * | 2018-09-30 | 2019-08-27 | 多鲜食品(昆山)有限公司 | 一种面包类食品用台式atp生物荧光检测装置 |
| CN209590035U (zh) * | 2019-02-20 | 2019-11-05 | 重庆科斯迈生物科技有限公司 | 化学发光免疫分析检测暗箱 |
| CN110554207A (zh) * | 2019-08-22 | 2019-12-10 | 中国科学院苏州生物医学工程技术研究所 | 光学检测装置 |
| CN110763836A (zh) * | 2019-12-26 | 2020-02-07 | 潍坊市康华生物技术有限公司 | 一种孵育盘暗室检测装置 |
| CN110794154A (zh) * | 2019-11-12 | 2020-02-14 | 广州科方生物技术股份有限公司 | 化学发光免疫分析仪及其检测方法 |
| CN211148444U (zh) * | 2019-11-15 | 2020-07-31 | 成都宜乐芯生物科技有限公司 | 一种带快门机构的pmt检测装置 |
| CN211825628U (zh) * | 2019-11-14 | 2020-10-30 | 天津精益宽谱科技有限公司 | 一种具有快速更换样品功能的光谱分析仪 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5466731B2 (ja) * | 2012-06-21 | 2014-04-09 | 株式会社日立製作所 | 化学発光計測装置 |
| CN209946007U (zh) * | 2018-12-19 | 2020-01-14 | 苏州汶颢微流控技术股份有限公司 | Atp荧光检测仪 |
-
2021
- 2021-02-20 CN CN202110195361.6A patent/CN113009166B/zh active Active
- 2021-04-16 WO PCT/CN2021/087664 patent/WO2022174512A1/zh active Application Filing
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223218A (en) * | 1987-04-09 | 1993-06-29 | Kabushiki Kaisha Meidensha | Instrument for quantitative analysis |
| US20150177236A1 (en) * | 2013-03-15 | 2015-06-25 | Gold Standard Diagnostics | Combined chemiluminescence and elisa automated sample reader |
| CN107923924A (zh) * | 2015-08-27 | 2018-04-17 | 株式会社日立高新技术 | 自动分析装置 |
| CN110133314A (zh) * | 2018-02-08 | 2019-08-16 | 成都深迈瑞医疗电子技术研究院有限公司 | 全自动化学发光免疫分析仪 |
| CN209311331U (zh) * | 2018-09-30 | 2019-08-27 | 多鲜食品(昆山)有限公司 | 一种面包类食品用台式atp生物荧光检测装置 |
| CN209148550U (zh) * | 2018-11-09 | 2019-07-23 | 广州军区广州总医院 | 一种纸电化学发光芯片用避光检测装置 |
| CN209590035U (zh) * | 2019-02-20 | 2019-11-05 | 重庆科斯迈生物科技有限公司 | 化学发光免疫分析检测暗箱 |
| CN110554207A (zh) * | 2019-08-22 | 2019-12-10 | 中国科学院苏州生物医学工程技术研究所 | 光学检测装置 |
| CN110794154A (zh) * | 2019-11-12 | 2020-02-14 | 广州科方生物技术股份有限公司 | 化学发光免疫分析仪及其检测方法 |
| CN211825628U (zh) * | 2019-11-14 | 2020-10-30 | 天津精益宽谱科技有限公司 | 一种具有快速更换样品功能的光谱分析仪 |
| CN211148444U (zh) * | 2019-11-15 | 2020-07-31 | 成都宜乐芯生物科技有限公司 | 一种带快门机构的pmt检测装置 |
| CN110763836A (zh) * | 2019-12-26 | 2020-02-07 | 潍坊市康华生物技术有限公司 | 一种孵育盘暗室检测装置 |
Non-Patent Citations (5)
| Title |
|---|
| LI LIQING: "Chemiluminescence analysis and its instrumentation", JOURNAL OF TAIAN TEACHERS COLLEGE, no. 6, 15 December 1997 (1997-12-15), pages 80 - 81, XP055959420 * |
| LIU HAIZHU; YAO JIJI; LIU PENG; WANG XIAOWEI; WANG MINGYI: "AutolumiS 3000 Automatic Microparticle Chemiluminescence Analyzer Structural Design of Sample Dosing System", CHINA MEDICAL EQUIPMENT, vol. 16, no. 11, 30 November 2019 (2019-11-30), pages 190 - 192, XP055959418, ISSN: 1672-8270, DOI: 10.3969/J.ISSN.1672-8270.2019.11.050 * |
| P. NOVO, V. CHU, J.P. CONDE: "Integrated optical detection of autonomous capillary microfluidic immunoassays:a hand-held point-of-care prototype", BIOSENSORS AND BIOELECTRONICS, vol. 57, 18 February 2014 (2014-02-18), Amsterdam , NL , pages 284 - 291, XP055548674, ISSN: 0956-5663, DOI: 10.1016/j.bios.2014.02.009 * |
| YAN YONGCUN, SHI PENGFEI, SONG WEILING, BI SAI: "Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy: In Vitro and In Vivo Perspectives", THERANOSTICS, vol. 9, no. 14, 1 January 2019 (2019-01-01), AU , pages 4047 - 4065, XP055959423, ISSN: 1838-7640, DOI: 10.7150/thno.33228 * |
| YAO JICHENG;CONG HAIYAN;LIU PENG;YUAN XIAOYAN;QU YEMIN;SONG YU;MA SHUQING;WANG MINGYI: "Structure design and Function Analysis of Temperature Control System for Autolumis 3000 Chemiluminescence Analyzer", CHINESE JOURNAL OF CLINICAL PATHOLOGIST, vol. 11, no. 3, 30 September 2019 (2019-09-30), pages 183 - 185, XP055959417, ISSN: 1674-7151, DOI: 10.3969/j.issn.1674-7151.2019.03.018 * |
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