WO2022168952A1 - Nouvelle enzyme de prénylation - Google Patents
Nouvelle enzyme de prénylation Download PDFInfo
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- WO2022168952A1 WO2022168952A1 PCT/JP2022/004501 JP2022004501W WO2022168952A1 WO 2022168952 A1 WO2022168952 A1 WO 2022168952A1 JP 2022004501 W JP2022004501 W JP 2022004501W WO 2022168952 A1 WO2022168952 A1 WO 2022168952A1
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- WIPO (PCT)
- Prior art keywords
- arginine
- amino acid
- residue
- peptide
- acid sequence
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/10—Citrulline; Arginine; Ornithine
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- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
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- Enzymes And Modification Thereof (AREA)
Abstract
La présente invention concerne : une enzyme qui transfère un groupe prényle à un résidu arginine ou à un résidu analogue d'arginine dans un peptide, ou à l'arginine ou à un analogue d'arginine, l'enzyme comprenant une séquence d'acides aminés représentée par SEQ ID NO : 1 ou une séquence mutante de celle-ci ; un peptide qui peut être utilisé en tant que substrat pour l'enzyme ; et un peptide prénylé.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022579623A JPWO2022168952A1 (fr) | 2021-02-05 | 2022-02-04 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021017504 | 2021-02-05 | ||
| JP2021-017504 | 2021-02-05 |
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| Publication Number | Publication Date |
|---|---|
| WO2022168952A1 true WO2022168952A1 (fr) | 2022-08-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2022/004501 WO2022168952A1 (fr) | 2021-02-05 | 2022-02-04 | Nouvelle enzyme de prénylation |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPWO2022168952A1 (fr) |
| WO (1) | WO2022168952A1 (fr) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020080490A1 (fr) * | 2018-10-17 | 2020-04-23 | 国立大学法人東京大学 | Procédé de production de bibliothèque de peptides |
-
2022
- 2022-02-04 JP JP2022579623A patent/JPWO2022168952A1/ja active Pending
- 2022-02-04 WO PCT/JP2022/004501 patent/WO2022168952A1/fr active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020080490A1 (fr) * | 2018-10-17 | 2020-04-23 | 国立大学法人東京大学 | Procédé de production de bibliothèque de peptides |
Non-Patent Citations (4)
| Title |
|---|
| DATABASE UniProtKB/TrEMBL Uniprot; ANONYMOUS : "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KXS89939.1};", XP055956481 * |
| OKADA MASAHIRO, SUGITA TOMOTOSHI, AKITA KOHEI, NAKASHIMA YU, TIAN TIAN, LI CHANG, MORI TAKAHIRO, ABE IKURO: "Stereospecific prenylation of tryptophan by a cyanobacterial post-translational modification enzyme", ORGANIC & BIOMOLECULAR CHEMISTRY, ROYAL SOCIETY OF CHEMISTRY, vol. 14, no. 40, 28 October 2016 (2016-10-28), pages 9639 - 9644, XP055956490, ISSN: 1477-0520, DOI: 10.1039/C6OB01759B * |
| PARAJULI ANIRUDRA, KWAK DANIEL H., DALPONTE LUCA, LEIKOSKI NIINA, GALICA TOMAS, UMEOBIKA UGOCHUKWU, TREMBLEAU LAURENT, BENT ANDREW: "A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, VERLAG CHEMIE, vol. 55, no. 11, 7 March 2016 (2016-03-07), pages 3596 - 3599, XP055956487, ISSN: 1433-7851, DOI: 10.1002/anie.201509920 * |
| PHAN CHIN-SOON, MATSUDA KENICHI, BALLOO NANDANI, FUJITA KEI, WAKIMOTO TOSHIYUKI, OKINO TATSUFUMI: "Argicyclamides A–C Unveil Enzymatic Basis for Guanidine Bis-prenylation", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 143, no. 27, 14 July 2021 (2021-07-14), pages 10083 - 10087, XP055956492, ISSN: 0002-7863, DOI: 10.1021/jacs.1c05732 * |
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| JPWO2022168952A1 (fr) | 2022-08-11 |
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