WO2022165954A1 - Nutritional essence syrup of datong hemerocallis citrina and preparation process therefor - Google Patents

Abstract

A method for preparing a liquid product of a Datong Hemerocallis citrina extract, comprising the following steps: A) performing enzymolysis on raw materials consisting of Cordyceps militaris, Hemerocallis citrina, and Panicum miliaceum by using papain to obtain an enzymolysis product, namely the Datong Hemerocallis citrina extract; and B) collecting liquid components of the Datong Hemerocallis citrina extract, and preparing the liquid product of the Datong Hemerocallis citrina extract by using the liquid components of the Datong Hemerocallis citrina extract. The liquid product prepared by the method contains 18 common amino acids, wherein the content of 8 essential amino acids for human body is 120.46-218.71 mg/100 mL, and the content of cordycepin is 13.64-20.46 mg/100 g. The liquid product is a functional beverage that has comprehensive and balanced nutrition, is rich in cordycepin, has zero fat and low calorie, and contains 8 essential amino acids for human body.

Description

A kind of Datong daylily nutrient puree and preparation process thereof technical field

The invention relates to food and a preparation process, in particular to a Datong daylily nutrient puree and a preparation process thereof.

Background technique

Daylily (Hemerocallis citrina), also known as Hemerocallis, also known as golden needle, forget worry grass, is a perennial herb of the Liliaceae family. It has a history of cultivation and consumption in China for more than 3,000 years. Daylily refers to the large flower buds or freshly bloomed flowers taken during the flowering period of Hemerocallis from June to August. It is sweet and delicious, and it is good in both meat and vegetables. It is a unique vegetable in China. Day lily is rich in nutrients and contains carbohydrates, proteins, vitamins, inorganic salts and a variety of essential amino acids. It is a typical high-protein, low-calorie, vitamin and mineral-rich health vegetable. Known as the four treasures of Chinese dried vegetables. Compared with other vegetables, daylily is more prominent in the content of mineral elements such as Ca, P, Fe, Zn and Se, and crude fiber. As an edible flower, there are many common volatile components in daylily, among which a large number of alcohol and ester aroma substances give it a unique and pleasant flavor. In China, day lily has a long history of dietary and health care applications. "Compendium of Materia Medica" records that day lily can help the chest and diaphragm, soothe the five internal organs, lighten the body and improve eyesight, cure red urine, relieve irritable heat, eliminate alcohol plague, and make people happy and worry-free. ; "Southern Yunnan Materia Medica" records that day lily tonifies yin and blood, relieves low back pain, treats metrorrhagia and milk obstruction. Lecithin, the main component of day lily, is known as "cell protector" and "vascular scavenger". Modern scientific research has proved that daylily has various health functions such as inhibiting fibroblast proliferation, preventing cancer cell proliferation, oral antidepressant activity, and intraperitoneal injection promoting sleep activity. It has wide application prospects in the fields of medicine and diet. But at present, day lily is mostly eaten in soup, fried, fresh, dried, or made as a seasoning dish, and there are few research reports on other forms of development and utilization.

Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to Ascomycetes, Sclerotinia, Spheroides, Ergotaceae, and Cordyceps. Lung and kidney, nourishing and strengthening the body, anti-cancer, anti-fatigue, anti-aging, improving immunity and other effects, especially the medicinal value of cordycepin, cordycepic acid and cordyceps polysaccharide are the most significant. At present, products with Cordyceps militaris as raw materials are mostly presented in the form of alcohol and health care products, and the relatively single form is not conducive to consumption by all people.

Yellow rice (Panicum miliaceum), also known as yellow millet, millet, millet, is a small-seeded grain and forage crop of the genus Poaceae, mainly distributed in arid, semi-arid, and low soil fertility areas, with a short growth period. , cold-resistant, drought-resistant and barren-resistant, it is one of the most abundant small grains in Shanxi Province. Yellow rice is obtained by peeling the millet seeds, and its endosperm is rich in carbohydrates, fats, proteins, vitamins and mineral elements. The human body can utilize organic selenium. It is a natural green food with high iron, high zinc, high calcium and rich cellulose, rich in active substances of various physiological functions, and is a plant source with high nutritional value. In northern my country, there are yellow rice oil steamed buns, yellow rice steamed buns, yellow rice thick wine, etc. Yellow rice has a unique flavor in cooking rice, porridge, making zongzi, and making jujube cakes, especially fried cakes made of yellow rice noodles, cut cakes, etc. Cakes are more popular. It can also be used for medical purposes, and is listed as a food with therapeutic value in traditional Chinese medicine. Regular consumption of yellow rice food can effectively prevent and treat gastrointestinal tumors and coronary heart disease. In the arid regions of northern my country, yellow rice and its processed products have always been the main ration and health food in its producing areas. However, the existing product processing technology is simple, resulting in poor product taste and palatability, which is an important factor affecting the development and promotion of yellow rice products. Even the most widely used flavor snacks are mostly on the table of farmers.

Invention Disclosure

An object of the present invention is to provide a method for preparing a liquid product of daylily extract.

The method provided by the present invention comprises the following steps:

A) use papain to enzymolysis the raw material that is made up of Cordyceps militaris, daylily and yellow rice, and the enzymolysis product obtained is the daylily extract;

B) Collecting the liquid component of the daylily extract, and using the liquid component of the daylily extract to prepare a liquid product of the daylily extract.

In the above method, in step A, the ratio of the papain to the raw material is 1600U/1g-3200U/1g.

In the above method, in step A, the enzymatic hydrolysis temperature of the papain is 55°C-60°C, and the enzymatic hydrolysis time is 2h-3h.

In the above-mentioned method, in step A,

The mass ratio of the day lily, the Cordyceps militaris and the yellow rice is 1-2:1-2:1-2.

Or, in step A, the mass fraction ratio of described daylily, described Cordyceps militaris and described yellow rice is 2:1-2:1-2;

Or, in step A, the mass fraction ratio of described daylily, described Cordyceps militaris and described yellow rice is 1:1-2:1-2.

Or, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 2:1:1;

Or, the mass fraction ratio of the day lily, Cordyceps militaris and yellow rice is 1:1:2;

Or, the mass fraction ratio of the day lily, Cordyceps militaris and yellow rice is 1:2:1;

Or, the mass fraction ratio of the day lily, Cordyceps militaris and yellow rice is 2:2:1;

Or, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 2:1:2.

In the above-mentioned method, in step A,

The Cordyceps militaris is the silkworm Cordyceps militaris; the Cordyceps militaris used in the present invention is a fresh live silkworm chrysalis, and a microsyringe is used to insert the Cordyceps militaris strain in the abdomen, and under a certain temperature, humidity and illumination, the silkworm Cordyceps militaris obtained through artificial cultivation .

In the above method, the daylily is freeze-dried daylily. The daylily used in the present invention, the raw material daylily is produced in Datong City, Shanxi Province. Datong City has a temperate semi-arid climate with sufficient sunshine and large temperature difference between day and night, which is especially suitable for the growth of daylily. Due to the zinc-rich and selenium-rich soil conditions formed by the volcanic eruption, the quality of Datong daylily produced ranks first in several major producing areas in the country. Datong day lily is nutritious, rich in protein and carbohydrates, especially calcium, phosphorus, iron, carotene, thiamine, niacin. , amino acid (8.43～9.12g/100g), rich selenium (0.0092～0.015mg/kg), rich zinc (36～40mg/kg), especially calcium (2.62×103～4.76×103mg/kg), iron (54 ~98mg/kg) content is very high, dietary fiber content is greater than 7.7g/100g.

The yellow rice is cooked yellow rice. The yellow rice used in the present invention is obtained after the yellow millet is shelled. The soil is deep and loose with good drainage. The growth period is 95 days. The 1000-grain weight is 7.9g.

In the above method, Cordyceps militaris, day lily and yellow rice are all in powder form, namely Cordyceps militaris powder, day lily powder and yellow rice powder.

In the above-mentioned steps A, the raw material that is made up of Cordyceps militaris, day lily and yellow rice is specifically as follows with papain enzymolysis:

1) Cordyceps militaris powder, day lily powder and yellow rice flour are mixed to obtain raw material; Add deionized water to raw material again, and the mass volume ratio of raw material and water is 1:20 (g/ml), obtains suspension;

2) enzymolysis with papain to obtain the enzymolysis product;

In the above method, in the step B, the preparation of the liquid product of the Datong day lily extract with the liquid components of the Datong day lily extract includes the following steps: the liquid of the Datong day lily extract is successively prepared with honey , colloid grinding, filtration, homogenization and sterilization to obtain a liquid product of Datong daylily extract.

The honey of the present invention is specifically purchased from Shanxi Shenlu Bee Products Co., Ltd. by using Sophora japonica nectar, and is rich in various nutrients such as glucose, fructose, vitamins, minerals and amino acids.

The product of the Datong day lily extract prepared by the above method is also within the protection scope of the present invention.

Another object of the present invention is to provide a method for preparing Datong daylily extract.

The method provided by the present invention includes step A in the above method.

Datong daylily extract prepared by step A in the above method.

The present invention aims at the shortage of deep processing technology of day lily, Cordyceps militaris and yellow rice at present and the blank of related deep processing products, based on the unique effect of Cordyceps militaris, and endows the day lily and yellow rice with higher utilization and functions, by combining traditional technology with Combining modern biological enzymatic hydrolysis technology, for the first time using daylily, Cordyceps militaris and yellow rice as the main raw materials, a kind of Datong daylily nutritional pulp was developed to achieve triple utilization of daylily, Cordyceps militaris and yellow rice, and make full use of our The provincial yellow flower industry and yellow rice miscellaneous grain resources have advantages to enhance the added value, create functional products with regional resource characteristics, extend the relevant industrial chain, protect the relevant value chain, and ensure the increase of farmers' income.

The present invention will be further described below in conjunction with the embodiments.

Description of drawings

Figure 1 shows the effect of different amounts of papain on the degree of protein hydrolysis (DH%).

Figure 2 shows the changes in the degree of protein hydrolysis in the enzymatic hydrolysis products at different temperatures.

Figure 3 shows the changes in the degree of protein hydrolysis in the enzymatic hydrolysis products under different enzymatic hydrolysis times.

The best way to implement an invention

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The materials, reagents, etc. used in the following examples can be obtained from commercial sources unless otherwise specified.

In the following description, numerous technical details are set forth for a better understanding of the present application. However, those skilled in the art can understand that even without these technical details and various changes and modifications based on the following embodiments, the technical solutions of the present application can be realized.

The preparation methods in the following examples are conventional methods unless otherwise specified.

The daylily in the following examples is freeze-dried daylily, if no special instructions, purchased from Datong Binghua Food Co., Ltd., and the company's daylily is the freshly picked yellow flower of the organic daylily production base in Yunzhou District, Datong, Shanxi Province. The vegetable is freeze-dried, and the dietary fiber content of the day lily is more than 7.7g/100g.

In the following examples, the flour is commercially available wheat flour (Hualong Farmhouse special refined wheat flour); the yeast is Angel high-activity dry yeast (the number of viable cells ≥ 20 billion/g).

The silkworm (Bombyx mori Linnaeus) pupa in the following examples is recorded in the following documents: Guo Shang, Xu Lina, Li Yanting, Zhou Wei, Chen Nan, Zhang Shengwan, Li Yinsheng. High-efficiency cultivation technology of silkworm Cordyceps militaris. Edible fungi, 2019, 41(3):46-48.

The Cordyceps militaris strain adopted in the following examples is the No. 8 Cordyceps militaris strain in the following documents: Guo Shang, Xu Lina, Li Yanting, Zhou Wei, Chen Nan, Zhang Shengwan, Li Yinsheng. Screening of the excellent strains of Cordyceps militaris [J] . Journal of Shanxi Agricultural University (Natural Science Edition), 2020, 40(01): 17-21.

Cordyceps militaris liquid medium in the following examples: 20 g of potato, 20 g of glucose, 20 g of protein, 1 g of KH ₂ PO ₄ , 0.5 g of MgSO ₄ , 1 000 mL of distilled water, natural pH, sterilized at 121° C. for 30 min.

Cordyceps militaris liquid strain (concentration 30% spore liquid) in the following examples: Cordyceps militaris liquid strain obtained by culturing Cordyceps militaris in Cordyceps militaris liquid medium, wherein the spore liquid with a concentration of 30%.

Unless otherwise specified in the following examples, the sensory tests are all single-blind, and 100 experimenters are selected to try and score their taste, with a full score of 100. Taking the average score as the test result, the experimenter is a relevant professional in the field.

Embodiment 1, the preparation of Datong day lily extract

1. Extract formula:

Daylily powder 2.0 parts by weight

Cordyceps militaris powder 1.0 parts by weight

Yellow rice flour 1.0 parts by weight

2. Preparation of the extract

1. Preparation of extract raw materials

Select freeze-dried daylily, silkworm Cordyceps militaris, yellow rice;

1) Cordyceps militaris powder is prepared according to the following method:

Fresh live silkworm (Bombyx mori Linnaeus) pupa, adopts microsyringe to inoculate Cordyceps militaris liquid strain (concentration 30% spore liquid) in abdomen, after inoculation, seal bottle mouth immediately;

Mycelium culture: After the inoculation is completed, the bacteria are placed on the bed frame one by one to develop bacteria. The bacteria will last for about 20 days, and the temperature should be kept at 15°C to 18°C and shaded from light until the surface of the pupa and the substrate is covered with white spots.

Substation culture: After the mycelium matures, it gradually turns from white to orange, indicating that the vegetative growth of the mycelium has been completed. The optimum temperature for the growth of sub-segments is 10°C to 25°C. At this time, increase the light and at the same time give a temperature difference of about 10 °C to stimulate the large color change. When the surrounding pupa and the surface of the substrate are accompanied by dome-shaped orange-yellow protuberances of different sizes, the sub-seat begins to form. At this time, the indoor temperature is maintained at 18°C to 23°C, and the relative air humidity is 80% to 90%. Too much humidity is easy to produce aerial mycelium, which is unfavorable to the growth of fruit bodies; too low humidity is easy to dehydrate the medium and affect the yield. After the sub-seat is formed, the direction of the light source should be adjusted appropriately according to the actual situation to ensure uniform light reception.

Appropriate ventilation is required throughout the incubation period, but the sealing plastic film cannot be removed. A needle can be used to puncture a small hole in the film to facilitate gas exchange.

Under normal management conditions, it takes about 40 days from inoculation to maturity of ascus, and about 20 days from kink of mycelium to maturity of fruit body. The biological efficiency is around 30%. When the sub-seat is orange-red or orange-yellow rod-shaped, with a height of 5-8cm, a cracked pattern appears on the head, and a yellow powder can be seen on the surface.

The harvested silkworm Cordyceps militaris is naturally dried at room temperature (25° C.), then pulverized, and passed through a 60-mesh sieve to obtain Cordyceps militaris powder.

2) Daylily powder

The daylily powder is to pulverize the freeze-dried daylily and pass through a 60-mesh sieve to obtain the daylily powder (particle size is less than 250 μm);

3) Yellow rice noodles

The yellow rice flour is obtained by pulverizing the cooked yellow rice and passing through a 60-mesh sieve to obtain the yellow rice flour.

Ripe yellow rice: Heat the commercial yellow rice with slow fire, stir-fry until the surface is slightly yellow fire color, and the inherent aroma escapes to obtain the ripe yellow rice; the specific conditions are to fry the yellow rice with a slow fire (160-170 ℃) to make the water lose. The rate is about 1.5%.

2. Enzymatic hydrolysis

The day lily powder, Cordyceps militaris powder and yellow rice flour obtained in the above-mentioned 1 are mixed according to the formula of the above-mentioned one (being 500g, 250g, 250g respectively) to obtain the raw material; Add deionized water to the raw material again, and the ratio of the raw material and the water is 1: 15 (W/V; g/ml) to obtain a suspension; then place the above suspension in an enzymolysis tank, add 0.2% (mass percent, specifically papain quality g/raw material quality g) papaya Protease (Solarbio Company, Lot No.1014K025, 800,000 u/g, ratio of enzyme activity to raw material 1600U/1g) was enzymatically hydrolyzed at 60°C for 3h to obtain the enzymatic hydrolysis product. During the enzymatic hydrolysis reaction, stirring was interrupted to make the enzymatic hydrolysis sufficient.

3. Allocation

The 15L enzymolysis product obtained in above-mentioned 2 was subjected to plate centrifugation (drum rotation speed 2500r/min, swiveling diameter 300mm), and the liquid was collected; then the above-mentioned liquid was poured into the mixing tank and heated to 55°C; 1% Sophora japonica nectar was added with stirring again. (The quality of Sophora japonica nectar is g/ml of liquid volume, purchased from Shanxi Shenlu Bee Products Co., Ltd.), and the preparation is carried out; after colloid grinding, it is passed through a wire mesh filter (pore size 0.2mm), a precision filter (retention pore size 0.2μm) ) was filtered and the filtrate was collected.

4. Homogeneous

The filtrate obtained in the above 3 was homogenized at 60° C. under a pressure of 40 Mpa for 1 min to obtain a mixed solution.

Through the homogenization operation, the suspended particles in the mixed liquid are further broken, uniform in size, without layering and precipitation, and uniformly and stably dispersed in the mixed liquid to maintain the uniform turbidity of the mixed liquid.

5. Sterilization

The mixture obtained in the above 4 was subjected to ultra-high temperature instantaneous sterilization, sterilized at 140 ° C for 4 s, and the outlet temperature was 50 ° C;

6. Filling

The sterilized mixed solution obtained in the above 5 was subpackaged in an aluminum-plastic flexible package under aseptic conditions, and the finished product was the prepared extract, which was named as Datong Daylily Nutrient Puree.

Embodiment 2, the preparation of Datong day lily extract

1. Extract formula:

Daylily powder 1.0 parts by weight

Cordyceps militaris powder 1.0 parts by weight

Yellow rice flour 2.0 parts by weight

Second, the preparation method of the extract

1. Preparation of extract raw materials

In the same preparation method as in Example 1, day lily powder, Cordyceps militaris powder and yellow rice powder were obtained.

2. Enzymatic hydrolysis

The day lily powder, Cordyceps militaris powder and yellow rice flour obtained in the above-mentioned 1 are mixed according to the formula of the above-mentioned 1 (being 250g, 250g, 500g respectively) to obtain the raw material; Add deionized water to the raw material again, and the proportioning of the raw material and the water is 1: 20 (W/V; g/ml) to obtain a suspension; then place the above suspension in an enzymolysis tank, add 0.3% (mass percent, specifically papain quality g/raw material quality g) papaya Protease (Solarbio, Lot No. 1014K025, 800,000 u/g, ratio of enzyme activity to raw material 2400U/1g) was enzymatically hydrolyzed at 55°C for 2h to obtain an enzymatic hydrolysis product. During the enzymatic hydrolysis reaction, stirring was interrupted to make the enzymatic hydrolysis sufficient.

3. Allocation

The 20L enzymolysis product obtained in the above 2 was subjected to plate centrifugation (drum rotation speed 2500r/min, turning stock diameter 300mm), and the liquid was collected; then the above-mentioned liquid was put into the mixing tank and heated to 50°C; 2% Sophora japonica nectar was added with stirring again. (glocophora nectar mass g/liquid volume ml), prepared, passed through a colloid mill and then filtered through a wire mesh filter (pore size 0.2 mm) and a precision filter (retention pore size 0.2 μm) to collect the filtrate.

4. Homogeneous

The filtrate obtained in the above 3 was homogenized at 70° C. under a pressure of 30 Mpa for 1 min to obtain a mixed solution.

Through the homogenization operation, the suspended particles in the mixed liquid are further broken, uniform in size, without layering and precipitation, and uniformly and stably dispersed in the mixed liquid to maintain the uniform turbidity of the mixed liquid.

5. Sterilization

The mixture obtained in the above 4 was subjected to ultra-high temperature instantaneous sterilization, sterilized at 140 ° C for 4 s, and the outlet temperature was 50 ° C;

6. Filling

The sterilized mixed solution obtained in the above 5 was subpackaged in an aluminum-plastic flexible package under aseptic conditions, and the finished product was the prepared extract, which was named as Datong Daylily Nutrient Puree.

Embodiment 3, the preparation of Datong day lily extract

1. Extract formula:

Daylily powder 1.0 parts by weight

Cordyceps militaris powder 2.0 parts by weight

Yellow rice flour 1.0 parts by weight

Second, the preparation method of the extract

1. Preparation of extract raw materials

In the same preparation method as in Example 1, day lily powder, Cordyceps militaris powder and yellow rice powder were obtained.

2. Enzymatic hydrolysis

The day lily powder, Cordyceps militaris powder and yellow rice flour obtained in the above-mentioned 1 are mixed according to the formula of the above-mentioned 1 (250g, 500g, 250g respectively) to obtain the raw material; Add deionized water to the raw material again, and the ratio of the raw material and the water is 1:10 (W/V; g/ml) to obtain a suspension; then place the above suspension in an enzymolysis tank, add 0.4% (mass percent, specifically papain quality g/raw material quality g) papaya Protease (Solarbio, Lot No. 1014K025, 800,000 u/g, ratio of enzyme activity to raw material 3200U/1g) was enzymatically hydrolyzed at 58°C for 3h to obtain an enzymatic hydrolysis product. During the enzymatic hydrolysis reaction, stirring was interrupted to make the enzymatic hydrolysis sufficient.

3. Allocation

The 9L enzymolysis product obtained in above-mentioned 2 was subjected to plate centrifugation (drum rotation speed 2500r/min, diameter of turning stock: 300mm) to collect the liquid; then the above-mentioned liquid was poured into the mixing tank and heated to 60°C; 1% Sophora japonica nectar was added with stirring again. (glocophora nectar mass g/liquid volume ml), prepared, passed through a colloid mill and then filtered through a wire mesh filter (pore size 0.2 mm) and a precision filter (retention pore size 0.2 μm) to collect the filtrate.

4. Homogeneous

The filtrate obtained in the above 3 was homogenized at 80° C. under a pressure of 40 Mpa for 1 min to obtain a mixed solution.

Through the homogenization operation, the suspended particles in the mixed liquid are further broken, uniform in size, without layering and precipitation, and uniformly and stably dispersed in the mixed liquid to maintain the uniform turbidity of the mixed liquid.

5. Sterilization

The mixture obtained in the above 4 was subjected to ultra-high temperature instantaneous sterilization, sterilized at 140 ° C for 4 s, and the outlet temperature was 50 ° C;

6. Filling

The sterilized mixed solution obtained in the above 5 was subpackaged in an aluminum-plastic flexible package under aseptic conditions, and the finished product was the prepared extract, which was named as Datong Daylily Nutrient Puree.

Embodiment 4, the preparation of Datong day lily extract

1. Extract formula:

Daylily powder 2.0 parts by weight

Cordyceps militaris powder 2.0 parts by weight

Yellow rice flour 1.0 parts by weight

Second, the preparation method of the extract

1. Preparation of extract raw materials

In the same preparation method as in Example 1, day lily powder, Cordyceps militaris powder and yellow rice powder were obtained.

2. Enzymatic hydrolysis

The day lily powder, Cordyceps militaris powder and yellow rice flour obtained in the above-mentioned 1 are mixed according to the formula of the above-mentioned 1 (500g, 500g, 250g respectively) to obtain the raw material; Add deionized water to the raw material again, and the ratio of the raw material and the water is 1:15 (W/V; g/ml) to obtain a suspension; then place the above suspension in an enzymolysis tank, add 0.2% (mass percent, specifically papain quality g/raw material quality g) papaya Protease (Solarbio, Lot No. 1014K025, 800,000 u/g, ratio of enzyme activity to raw material 1600U/1g) was enzymatically hydrolyzed at 60°C for 2h to obtain an enzymatic hydrolysis product. During the enzymatic hydrolysis reaction, stirring was interrupted to make the enzymatic hydrolysis sufficient.

3. Allocation

The 15L enzymolysis product obtained in the above 2 was subjected to plate centrifugation (drum rotation speed 2500r/min, turning stock diameter 300mm), and the liquid was collected; then the above-mentioned liquid was poured into the preparation tank and heated to 55 ° C; 2% Sophora japonica nectar was added with stirring again. (glocophora nectar mass g/liquid volume ml), prepared, passed through a colloid mill and then filtered through a wire mesh filter (pore size 0.2 mm) and a precision filter (retention pore size 0.2 μm) to collect the filtrate.

4. Homogeneous

The filtrate obtained in the above 3 was homogenized at 80° C. under a pressure of 30 Mpa for 1 min to obtain a mixed solution.

Through the homogenization operation, the suspended particles in the mixed liquid are further broken, uniform in size, without layering and precipitation, and uniformly and stably dispersed in the mixed liquid to maintain the uniform turbidity of the mixed liquid.

5. Sterilization

The mixed solution obtained in the above 4 was subjected to ultra-high temperature instantaneous sterilization, sterilized at 140°C for 4s, and the outlet temperature was 50°C;

6. Filling

The sterilized mixed solution obtained in the above 5 was subpackaged in an aluminum-plastic flexible package under aseptic conditions, and the finished product was the prepared extract, which was named as Datong Daylily Nutrient Puree.

Embodiment 5, the preparation of Datong day lily extract

1. Extract formula:

Cordyceps militaris powder 1.0 parts by weight

Daylily powder 1.0 parts by weight

Yellow rice flour 1.0 parts by weight

Second, the preparation method of the extract

1. Preparation of extract raw materials

In the same preparation method as in Example 1, day lily powder, Cordyceps militaris powder and yellow rice powder were obtained.

2. Enzymatic hydrolysis

The day lily powder, Cordyceps militaris powder and yellow rice flour obtained in the above-mentioned 1 are mixed according to the formula of the above-mentioned 1 (500g, 250g, 500g respectively) to obtain the raw material; Add deionized water to the raw material again, and the ratio of the raw material and the water is 1: 20 (W/V; g/ml) to obtain a suspension; then place the above suspension in an enzymolysis tank, add 0.4% (mass percent, specifically papain quality g/raw material quality g) papaya Protease (Solarbio Company, Lot No. 1014K025, 800,000 u/g, ratio of enzyme activity to raw material 3200U/1g) was enzymatically hydrolyzed at 55°C for 3h to obtain an enzymatic hydrolysis product. During the enzymatic hydrolysis reaction, stirring was interrupted to make the enzymatic hydrolysis sufficient.

3. Allocation

The 20L enzymolysis product obtained in above-mentioned 2 was subjected to plate centrifugation (drum rotation speed 2500r/min, turning stock diameter 300mm) to collect the liquid; then the above-mentioned liquid was put into the mixing tank and heated to 60°C; 2% Sophora japonica nectar was added with stirring again. (glocophora nectar mass g/liquid volume ml), prepared, passed through a colloid mill and then filtered through a wire mesh filter (pore size 0.2 mm) and a precision filter (retention pore size 0.2 μm) to collect the filtrate.

4. Homogeneous

The filtrate obtained in the above 3 was homogenized for 1 min at 60° C. under a pressure of 50 Mpa to obtain a mixed solution.

Through the homogenization operation, the suspended particles in the mixed liquid are further broken, uniform in size, without layering and precipitation, and uniformly and stably dispersed in the mixed liquid to maintain the uniform turbidity of the mixed liquid.

5. Sterilization

The mixed solution obtained in the above 4 was subjected to ultra-high temperature instantaneous sterilization, sterilized at 140°C for 4s, and the outlet temperature was 50°C;

6. Filling

The sterilized mixed solution obtained in the above 5 was subpackaged in an aluminum-plastic flexible package under aseptic conditions, and the finished product was the prepared extract, which was named as Datong Daylily Nutrient Puree.

Example 6. Optimization and exploration of parameters in the preparation method of extract

(1) Determination test of papain enzymolysis process parameters

1. One-factor experiment

(1) Investigation on the amount of papain (C)

The influence of the amount of papain on the degree of protein hydrolysis is within a certain range. Properly increasing the amount of papain can effectively improve the degree of protein hydrolysis.

The extraction method in Example 1 was adopted, except that the amount of papain was set to 0.1%≤C≤0.2%, 0.2%≤C≤0.4%, 0.4%<C≤0.6%, and the remaining steps were the same. C is the percentage of papain mass g/raw material mass g.

The changes in the degree of protein hydrolysis in the enzymatic hydrolysis products were detected (method reference: Deng Guilan, Li Junping, Development of Pumpkin and Mung Bean Compound Beverage, Beverage Industry, 2017, 20(4): 28-34.), the results are shown in Figure 1.

The sensory evaluation of the extracts (Datong daylily nutritional puree) obtained with different amounts of papain, the results are shown in Table 1.

Table 1 is the sensory evaluation of Datong daylily nutritional pulp with different amounts of papain

According to Figure 1 and Table 1, when the amount of papain is in the range of 0.1%-0.4%, the degree of protein hydrolysis increases significantly with the increase of the amount of enzyme. When the amount of papain reaches 0.4%, the degree of protein hydrolysis changes gently, while when When the enzyme dosage is 0.4% < C ≤ 0.6%, the nutritional pulp of Datong day lily is brown, has a general taste, and has a bitter and astringent taste. Therefore, it is more appropriate to choose an enzyme dosage of 0.2% ≤ C ≤ 0.4%, that is, the enzyme activity and the raw material. The ratio is 1600U/g-3200U/g.

(2) Investigation on the enzymatic hydrolysis temperature (t)

The optimum temperature of papain is generally around 50-60 ℃, and the enzymatic hydrolysis temperature will have a great impact on the protein hydrolysis degree and product flavor of Datong day lily nutritional pulp.

The extraction method in Example 1 was adopted, except that the enzymatic hydrolysis temperature (t) was set to 50°C≤t<55°C, 55°C≤t≤60°C, and 60°C<t≤65°C, and the remaining steps were the same.

The changes in the degree of protein hydrolysis in the enzymatic hydrolysis products were detected, and the results are shown in Figure 2.

The sensory evaluation of the extract (Datong daylily nutritional puree) obtained at different enzymatic hydrolysis temperatures, the results are shown in Table 2.

Table 2 is the sensory evaluation of Datong daylily nutritional puree at the enzymatic hydrolysis temperature

According to Figure 2 and Table 2, in the temperature range of 50-60 °C, the degree of protein hydrolysis gradually increases with the increase of temperature, and the degree of hydrolysis reaches the maximum when the temperature is 55 °C≤t≤60 °C. increased, the degree of hydrolysis decreased. When the enzymatic hydrolysis temperature is 50℃≤t<55℃, the nutrient pulp of Datong daylily is slightly layered, the aroma is insufficient, and there is a little miscellaneous smell, while when the enzymolysis temperature is 60℃<t≤65℃, the Datong daylily can be Nutrient puree had an average taste, with a bitter and astringent taste, which reduced its sensory evaluation. Therefore, 55℃≤t≤60℃ was chosen as the optimum enzymatic hydrolysis temperature.

(3) Investigation on the enzymatic hydrolysis time (t)

The extraction method in Example 1 was adopted, except that the enzymatic hydrolysis time (t) was set as 1h≤t<2h, 2h≤t≤3h, 3h<t≤4h, and the remaining steps were the same.

The changes in the degree of protein hydrolysis in the enzymatic hydrolysis products were detected, and the results are shown in Figure 3.

The sensory evaluation of the extract (Datong daylily nutritional puree) obtained by different enzymolysis time, the results are shown in Table 3.

Table 3 is the sensory evaluation of Datong daylily nutritional puree with enzymatic hydrolysis time (t)

According to Figure 3 and Table 3, the enzymatic hydrolysis time is in the range of 1-3h, and the degree of proteolysis rises rapidly with the extension of time. When 2h≤t≤3h, the proteolysis rate has become larger, and the proteolysis time continues to be prolonged. The increasing trend of the degree of hydrolysis weakened and tended to be gentle, and when the enzymatic hydrolysis time was 3h<t≤4h, the nutritional pulp of Datong daylily was brown and the taste decreased, so it is better to choose the hydrolysis time as 2h≤t≤3h.

2. Orthogonal test

Taking papain dosage, enzymatic hydrolysis temperature and enzymatic hydrolysis time as three factors, the L ₉ (3 ⁴ ) orthogonal table was selected and used for orthogonal experiment, and the protein hydrolysis degree was used as the index to determine the optimal conditions of papain hydrolysis, Each experimental combination was repeated three times, and the average value was taken as the result.

Table 4 is the factor level table of the orthogonal test

Table 5 shows the results of the orthogonal test of the optimal conditions for papain enzymatic hydrolysis

Table 6 is the analysis of variance table of orthogonal test for optimal conditions of papain enzymatic hydrolysis

From the analysis of the experimental data in Table 5 and Table 6, it can be seen that the factors affecting the enzymatic hydrolysis effect of papain are arranged in descending order of the amount of papain > enzymatic hydrolysis temperature > enzymatic hydrolysis time. The optimal enzymatic hydrolysis conditions are A ₂ B ₂ C ₂ , that is, the amount of papain is 0.2%-0.4%, that is, the ratio of enzyme activity to raw materials is 1600U/g-3200U/g, and the enzymatic hydrolysis temperature is 55℃-60℃. The enzymatic hydrolysis time was 2h-3h, and the protein hydrolysis degree was about 16.144% under this condition.

(2) Comparison of nutritional components of daylily under different treatments

The test materials were picked from the organic daylily production base in Yunzhou District, Datong, Shanxi Province. Materials include fresh daylily, dried daylily, and freeze-dried daylily.

1. Material pretreatment

Drying daylily: Freshly picked daylily in the organic daylily production base in Yunzhou District, Shanxi Simulated natural drying environment: 105°C for 15 minutes, 40°C for 8 hours, drying 100g fresh daylily to get 16.2g sample.

Freeze-dried daylily: The freshly picked daylily from the organic daylily production base in Yunzhou District, Datong, Shanxi is freeze-dried in the following manner. The specific operation procedure is shown in Table 7 below, and the vacuum pump is started from stage 2.

Table 7 is the operating procedure

stage	duration/h	temperature/℃
1	3	-55
2	1	-50

3	1	-45
4	1	-40
5	1	-35
6	1	-30
7	2	-25
8	2	-20
9	2	-15
10	2	-10
11	1	-5
12	1	0
13	1	5
14	1	10
15	1	15
16	2	20
17	2	25
18	2	30

2. Test method

The various processing materials of the above 1 are detected according to the following methods, and the soluble protein content is according to Coomassie brilliant blue G-250 staining method (BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal.Biochem.1976,(72):248–254.), the total sugar content adopts NDS method (AURISANO N, BERTANI A, REGGIANI R. Involvement of calcium and calmodulin in protein and amino acid metabolism in rice roots under anoxia. Plant Cell Physiol, 1995, 36: 1087-1088. DOI: 10.1080/11263509509440949.), the content of flavonoids was determined by sodium nitrite and aluminum nitrate colorimetry (Geng Linlin. Study on the extraction of total flavonoids from daylily with ultrasonic coordinated electrostatic field[D].2017 , South China University of Technology, Master's Thesis.), ascorbic acid using molybdenum blue colorimetry (Gao Junfeng. Plant Physiology Experiment Guide [M]. Beijing: Higher Education Press, 2006, 211-219). After the fresh day lily was dried, it was found that 1 g of dried samples was equivalent to 6.17 g of fresh samples, and 1 g of freeze-dried samples was equivalent to 5.66 g of fresh samples. Under the conditions of equal quality, the nutrient content in the samples with different treatments (Table 8) The result is as follows:

As can be seen from Table 8, the soluble protein content in fresh day lily was 2.17 mg g ^-1 , and compared with the fresh sample, after the day lily was freeze-dried, the soluble protein content was 1.99 mg g ^-1 , and there was no significant change; and After drying the day lily, its soluble protein content was 0.24 mg g ^-1 , and the difference was significant.

As can be seen from Table 8, the total sugar content of fresh day lily was 8.9%, while the total sugar content of drying treatment and freeze-drying treatment were 10.21% and 10.23%, respectively. Compared with the fresh sample, the total sugar content was significantly increased. .

As can be seen from Table 8, the flavonoid content of fresh day lily was 20.53 mg 100 g ^-1 , while the flavonoid content of drying treatment and freeze-drying treatment were 8.06 and 18.66 mg g ^-1 , respectively.

As can be seen from Table 8, the ascorbic acid content in the fresh day lily is 0.81mg 100g ^-1 , the freeze-drying treatment class ascorbic acid content is 0.76mg g ^-1 , and the content in the drying sample is 0.24mg 100g ^-1 , which is different from the fresh sample, The lyophilized phase changed significantly.

Table 8 is the nutrient content under the condition of equal quality

It can be seen from Table 8 that the total sugar content in the freeze-dried daylily samples is higher than that of the fresh samples, while compared with the dried samples, the contents of soluble protein, total flavonoids and ascorbic acid components in the freeze-dried samples are not significantly different from those of the fresh samples. Therefore, under the inherent deficiencies that the daylily has a short picking period and cannot be produced annually, the present invention uses the freeze-dried sample as a source of raw materials, which makes up for the deficiency that restricts the annual processing of the daylily.

Freeze-dried daylily has passed the acute oral toxicity test of GB15193.3-2014 national food safety standard, and the result shows that the acute oral LD50 of the sample to ICR mice is >5000mg/kg. According to the acute toxicity dose classification, this sample belongs to the practical non-toxic grade.

(3) Comparison of nutritional components of yellow rice under different treatment methods

1. Pretreatment of yellow rice:

(1) Stir-fried yellow rice (cooked yellow rice): the yellow rice is heated at a slow fire, stir-fry until the color changes 7-8 and mature, and the inherent aroma escapes to obtain the cooked yellow rice; , the water loss rate is 1.5%, and it is ground into powder;

(2) Raw yellow rice: commercial yellow rice is directly ground into powder;

(3) Soaking yellow rice: commercial yellow rice was soaked in water (mass ratio of rice:water=1:1) for 12 hours, and refined.

2. Test method

Enzymatic hydrolysis method for starch content determination (refer to GB 5009.9-2016 Determination of starch content in food); enzymatic hydrolysis method for slow-digestible starch content determination (refer to Miao Ming et al.'s discussion on the in vitro determination of slow-digestible starch); soluble The Coomassie brilliant blue method was used for the determination of protein content; the acid hydrolysis method was used for the determination of fat content; the enzymatic hydrolysis method was used for the determination of dietary fiber content (refer to "Determination of Dietary Fiber Content in Foods" in GB 5009.88-2014); References Liu Yanfang et al. Research on the determination method of total flavonoids of medicinal fungi Phellinus linteus).

The result is as follows:

Table 9 is the comparison of nutritional components of yellow rice under different treatment methods

Table 10 Sensory evaluation of the single-blind experiment of yellow rice with different treatments

As can be seen from Table 9, the nutrient content of fried yellow rice and dried yellow rice is similar, all is higher than soaked yellow rice, and as can be seen from Table 10, fried yellow rice sensory evaluation is higher than dried yellow rice, so choose fried yellow rice.

(4) The effect of different medium on the nutrition of Cordyceps militaris fruiting body

Species: Cordyceps militaris (Cordyceps militaris) No. 8 strain of Cordyceps militaris.

Culture medium: silkworm chrysalis, rice, wheat.

Cordyceps militaris liquid medium: potato 20g, glucose 20g, protein 20g, KH ₂ PO ₄ 1g, MgSO ₄ 0.5g, distilled water 1 000mL, natural pH, sterilized at 121°C for 30min.

Solid medium: put 30 g of the culture medium (rice or wheat) into a culture bottle, add 30 mL of Cordyceps militaris liquid medium, and sterilize at 121° C. for 30 minutes.

1. Method of cultivating fruiting bodies

1) Inoculation

The inoculation tools, the outer wall of the bacterial culture bottle, the hands of the operator, etc., should be wiped or dipped with 75% alcohol for disinfection. Before inoculation, use high-quality aerosol disinfectant or conventional methods to seal and disinfect the inoculation room for 30 minutes, and then wear disinfected clothes and enter the inoculation room for inoculation.

Silkworm chrysalis matrix inoculation: select fresh live silkworm chrysalis during inoculation, adopt microsyringe to inoculate liquid strain of Cordyceps militaris (30% spore liquid of concentration) in abdomen, inoculum amount is suitable (every about 0.5mL) with pupa body full without rupture, After inoculation, seal the bottle mouth tightly immediately;

Rice or wheat solid substrate inoculation: use a sterilized micro-sprayer to evenly spray the liquid strain of Cordyceps militaris on the surface of the substrate (rice or wheat), each bottle is inoculated with 3mL to 5mL of liquid seed, and immediately seal the bottle mouth after inoculation.

2) Germination management

Mycelium culture: After the inoculation is completed, the bacteria are placed on the bed frame one by one to develop bacteria. The bacteria will last for about 20 days, and the temperature should be kept at 15°C to 18°C and shaded from light until the surface of the pupa and the substrate is covered with white spots.

Substation culture: After the mycelium matures, it gradually turns from white to orange, indicating that the vegetative growth of the mycelium has been completed. The optimum temperature for the growth of sub-segments is 10°C to 25°C. At this time, increase the light and at the same time give a temperature difference of about 10 °C to stimulate the large color change. When the surrounding pupa and the surface of the substrate are accompanied by dome-shaped orange-yellow protuberances of different sizes, the sub-seat begins to form. At this time, the indoor temperature is maintained at 18°C to 23°C, and the relative air humidity is 80% to 90%. Too much humidity is easy to produce aerial mycelium, which is unfavorable to the growth of fruit bodies; too low humidity is easy to dehydrate the medium and affect the yield. After the sub-seat is formed, the direction of the light source should be properly adjusted according to the actual situation to ensure uniform light reception. Appropriate ventilation is required throughout the incubation period, but the sealing plastic film cannot be removed. A needle can be used to puncture a small hole in the film to facilitate gas exchange.

3) Harvest

Under normal management conditions, it takes about 40 days from inoculation to maturity of ascus, and about 20 days from kink of mycelium to maturity of fruit body. The biological efficiency is around 30%. When the sub-seat is orange-red or orange-yellow rod-shaped, with a height of 5-8cm, a crack-like pattern appears on the head, and yellow powder is visible on the surface, it should be harvested in time. The obtained silkworm Cordyceps militaris.

2. Detection of biologically active ingredients

1) Determination of Cordyceps polysaccharide content

References for detection methods: Shao Bo, Liu Boyan, Chen Liuxin, Wen Yuanyuan, Wang Xiaoyan, Zhai Xiaotian, Zhou Zheng, Li Ying, Zhao Yanan, Si Yanhong, Qin Shucun. The mechanism of Cordyceps militaris polysaccharide-lowering cholesterol-lowering effect[J].Chinese Journal of Arteriosclerosis,2020 , 28(10):861-866.

Preparation of glucose standard curve: take 20 mg of glucose dried to constant weight at 105 °C, put it in a small beaker, add distilled water to dissolve, then transfer to a 500 mL volumetric flask, add water to make the volume, that is, 40 ug/mL glucose standard solution. Take 40ug/mL glucose solution 0, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8 mL to 9 20 mL stopper test tubes numbered 0-8, add 1.8 mL of distilled water, 6% 1.6mL of phenol solution and 7.5mL of concentrated sulfuric acid, shake well, let stand for 10min, shake well, (23±2) ℃ for 20min, set 3 parallel samples in each group, measure the absorbance of the samples with a UV spectrophotometer at a wavelength of 490nm . The abscissa is the number of micrograms of glucose, the ordinate is the absorbance, and the standard curve of glucose was made by excel software.

Determination of sample polysaccharide content: Take 0.2mL of extract from a 50mL volumetric flask with a pipette to 10 20mL stoppered test tubes, prepare another 20mL stoppered test tube as a control, add to 11 stoppered test tubes. Distilled water was made up to 1.8mL. Add 1.6 mL of 6% phenol solution and 7.5 mL of concentrated sulfuric acid to a 20 mL stoppered test tube, shake well, and let stand for 10 minutes; shake well again, and place at (23±2) °C for 20 minutes, then measure the absorbance at 490 nm.

Sample polysaccharide content//(mg/g)_[polysaccharide content in extract (ug) x dilution factor x 100]/[sample dry weight (g) x 1 000]

2) Determination of cordycepin content

For the detection method, please refer to the reference: Li Jianping, Zhang Tie, Zeng Wenbo, Ma Xiaoshuang. Analysis of nucleosides from Cordyceps militaris from different sources [J]. China Edible Fungi, 2018, 37(05): 49-56.

Weigh 2.00 g of the crushed sample into a centrifuge tube, add 15 mL of distilled water, sonicate in a 50°C water bath for 60 min, and centrifuge to take the supernatant; then add 5 mL of distilled water to the centrifuge tube, mix well, and sonicate in a water bath for 30 min. Centrifuge to take the supernatant; then add 5 mL of distilled water, mix evenly in a water bath and ultrasonicate for 30 minutes, centrifuge to take the supernatant, combine the supernatants from the three centrifugations, dilute to 25 mL with distilled water, filter the membrane for measurement, if it is cloudy again Filter the membrane after centrifugation. Detection was carried out by ultra-high liquid chromatography.

3) Determination of Cordyceps Acid Content

For the detection method, please refer to the reference: Li Jianping, Zhang Tie, Zeng Wenbo, Ma Xiaoshuang. Analysis of nucleoside components of Cordyceps militaris from different sources [J]. China Edible Fungi, 2018, 37(05): 49-56.

The extraction process is the same as the cordycepin assay. Detection was carried out by ultra-high liquid chromatography.

4) Determination of adenosine content

For the detection method, see Zhu Lina, Liu Yanfang, Zhang Hongxia, Zhou Shuai, Zhang Zhong, Li Chuanhua, Gao Xinhua, Tang Qingjiu. Comparison of active components of fruiting bodies of Cordyceps militaris from different sources [J]. Journal of Mycology, 2018,37(12):1695- 1706.

Detection by liquid chromatography.

The main biologically active substances in Cordyceps militaris are cordycepin, cordycepic acid, adenosine, and cordyceps polysaccharide. Different cultivation substrates will affect the content of the main active substances of Cordyceps militaris, so using these four indicators as the evaluation basis, the active components of the fruiting bodies of Cordyceps militaris cultivated in three different substrates are compared and analyzed, and it is finally determined that Cordyceps militaris is the best. Add ingredients. The test results are as follows:

Table 11 shows the effects of different media on the nutrition of the fruiting bodies of Cordyceps militaris

It can be seen from Table 11 that the medium composition has a greater impact on the bioactive components in the fruiting body of Cordyceps militaris, and silkworm chrysalis is used for inoculation, and its nutrient composition is much higher than that of the rice and wheat substrates.

To sum up, freeze-dried yellow flowers, silkworm Cordyceps militaris and fried yellow rice have the best nutritional components, so these three are used as the main raw materials for further research in this project.

Embodiment 7, the sensory evaluation of Datong daylily nutrition puree

In this example, 100 relevant professionals in the field were selected as experimenters to test the present invention respectively.

The Datong daylily nutrient puree prepared in Examples 1-5 was tried, and its mouthfeel was scored, with a full score of 100 points. The evaluation criteria are shown in Table 12. Taking the average score as the test result, the evaluation test results are shown in Table 13.

Table 12 is the test evaluation standard

Table 13 is the evaluation test results

	average score/point
Example 1	94
Example 2	90
Example 3	93
Example 4	97
Example 5	93

It can be seen that the Datong daylily nutrient paste prepared by the present invention is light brown, pure in color, good in uniformity, not layered, has the unique taste and smell of daylily, Cordyceps militaris and yellow rice, has no peculiar smell, and has a sweet and sour taste. Suitable, with good organoleptic properties.

Embodiment 8. Determination of physical and chemical indexes of Datong daylily nutrition puree

The finished product physical and chemical index detection is carried out to the Datong day lily nutritional puree prepared by the preparation method embodiment 1-5 of the present invention, and the detection results are:

(1) Contains 18 kinds of common amino acids, of which the content of 8 kinds of essential amino acids (mg/100mL): 120.46-218.71, the content of tryptophan is determined according to GB/T 15400-2018, and the content of the remaining 7 kinds of essential amino acids is determined according to GB 5009.124-2016 The contents of 8 essential amino acids are: valine content (mg/100mL): 18.9-35.64, isoleucine content (mg/100mL): 7.9-13.41, leucine content (mg/100mL): 14.6- 26.51. Phenylalanine content (mg/100mL): 7.31-13.72, Lysine content (mg/100mL): 16.4-29.68, Methionine content (mg/100mL): 3.75-7.32, Threonine content (mg/100mL) 100mL): 20.5-35.22, tryptophan content (mg/100mL): 31.1-57.21.

(2) Cordycepin (mg/100g): 13.64-20.46, determined according to NY/T 2116-2012.

(3) Soluble solids: 5-10.2%, determined according to GB/T12143.

Embodiment 9. Determination of nutritional components of Datong daylily nutrient puree

The nutritional components of the Datong daylily nutrient puree prepared by the preparation method embodiment 1-5 of the present invention were detected, and the results were:

(1) Energy (kJ/100g): 69-88, measured according to the calculation method.

(2) Protein (g/100g): 0.5-1.1, measured according to the first method of GB5009.5-2016.

(3) Fat (g/100g): 0, measured according to the second method of GB5009.5-2016.

(4) Carbohydrates (g/100g): 3.5-4.2, the determination is based on the calculation method.

(5) Sodium (mg/100g): 0, determined according to the fourth method of GB5009.91-2017.

The above test results meet the national food-related standards.

It can be seen that the Datong daylily nutrient puree prepared by the present invention is a zero-fat, low-calorie, comprehensive and balanced nutritional functional drink rich in cordycepin and containing 8 kinds of essential amino acids of human body.

The above are only the preferred embodiments of the present invention. It should be pointed out that, without departing from the principles of the present invention, those of ordinary skill in the art can make several improvements and modifications, all of which should be regarded as the protection scope of the present invention.

Industrial application

Based on the nutritional properties and health care value of daylily, Cordyceps militaris and yellow rice, the invention aims to expand regional delicacies into national delicacies, and it is the first time to develop functional products with high added value through intensive processing.

After the material of the present invention is crushed, enzymatically hydrolyzed and centrifuged, the obtained supernatant is prepared, homogenized, sterilized and filled to obtain Datong daylily nutrient puree, and the precipitation is used for other purposes, so as to maximize the realization of the raw material. The full utilization of the production waste eliminates the pollution of the production waste to the environment, and has the following advantages:

(1) The method of the present invention is unique, the technological process is simple, and the operation is convenient. After the material is pulverized, enzymatically hydrolyzed, and centrifuged, the obtained supernatant is prepared, homogenized, sterilized, and filled to obtain Datong Daylily Nutrient puree , the precipitation is used for other purposes, which maximizes the full utilization of raw materials and eliminates the pollution of production waste to the environment.

(2) Datong daylily nutrient puree prepared by the present invention has the unique flavor of daylily, Cordyceps militaris and yellow rice, has no peculiar smell, and has better product uniformity and stable sensory quality; The tissue state is not delaminated, the color is light brown, and it has good sensory properties.

(3) The present invention selects high-protein and low-calorie day lily that is rich in vitamins and minerals, yellow rice with high dietary fiber content, and Cordyceps militaris containing various biologically active substances such as cordycepin and cordyceps polysaccharide through scientific and rational dietary matching technology. , as the main raw material for making Datong day lily nutritional puree, it can enhance the efficacy of a single variety, so that Datong daylily nutritional puree contains the nutrition, physiological activity and functional components of daylily, Cordyceps militaris and yellow rice at the same time. It has comprehensive nutritional and medicinal health care functions, comprehensive nutrition, high dietary fiber content and high protein content, so as to meet people's needs for healthy, nutritious and health-care functional foods.

(4) The product in the present invention contains 18 kinds of common amino acids, among which the content of 8 kinds of essential amino acids is 120.46-218.71mg/100mL, and the content of cordycepin is 13.64-20.46mg/100g. A low-calorie, comprehensive and balanced nutritional functional drink containing 8 essential amino acids for the human body.

(5) Datong day lily nutritional pulp prepared by the present invention has the functions of regulating immunity, promoting sleep, anti-oxidation, promoting gastrointestinal motility, maintaining normal structure and function of cardiovascular system, etc., and is suitable for drinking by all people. The Datong daylily nutrient puree prepared by the method has the characteristics of rich nutrition, sweet and sour taste and the like.

Claims (14)

1. A method for preparing a liquid product of a daylily extract, comprising the steps:

   A) use papain to enzymolysis the raw material that is made up of Cordyceps militaris, daylily and yellow rice, and the enzymolysis product obtained is the daylily extract;

   B) Collecting the liquid component of the daylily extract, and using the liquid component of the daylily extract to prepare a liquid product of the daylily extract.
2. The method of claim 1, wherein:

   In step A, the ratio of the papain to the raw material is 1600U/1g-3200U/1g.
3. The method according to claim 1 or 2, characterized in that:

   In step A, the enzymatic hydrolysis temperature of the papain is 55°C-60°C, and the enzymatic hydrolysis time is 2h-3h.
4. The method according to any one of claims 1-3, wherein:

   In step A, the mass fraction ratio of the day lily, the Cordyceps militaris and the yellow rice is 1-2:1-2:1-2;

   Or, in step A, the mass fraction ratio of described daylily, described Cordyceps militaris and described yellow rice is 2:1-2:1-2;

   Or, in step A, the mass fraction ratio of the day lily, the Cordyceps militaris and the yellow rice is 1:1-2:1-2.
5. The method according to any one of claims 1-4, wherein:

   In step A, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 2:1:1.
6. The method according to any one of claims 1-4, wherein:

   In step A, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 1:1:2.
7. The method according to any one of claims 1-4, wherein:

   In step A, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 1:2:1.
8. The method according to any one of claims 1-4, wherein:

   In step A, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 2:2:1.
9. The method according to any one of claims 1-4, wherein:

   In step A, the mass ratio of the day lily, Cordyceps militaris and yellow rice is 2:1:2.
10. The method according to any one of claims 1-9, wherein:

    In step A,

    Described cordyceps militaris is silkworm cordyceps militaris;

    Described daylily is freeze-dried daylily;

    The yellow rice is cooked yellow rice.
11. The method according to any one of claims 1-10, wherein:

    In the step B, the preparation of the liquid product of the Datong day lily extract with the liquid components of the Datong day lily extract includes the following steps: the liquid of the Datong day lily extract is successively subjected to honey modulation, colloid milling, Filtration, homogenization and sterilization are carried out to obtain a liquid product of Datong daylily extract.
12. The product of Datong daylily extract prepared by any one of claims 1-11.
13. A method for preparing Datong day lily extract, which comprises step A of any one of claims 1-11.
14. The Datong daylily extract prepared by the method of claim 13.

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