WO2022146242A1 - Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production - Google Patents

Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production Download PDF

Info

Publication number
WO2022146242A1
WO2022146242A1 PCT/TR2020/051429 TR2020051429W WO2022146242A1 WO 2022146242 A1 WO2022146242 A1 WO 2022146242A1 TR 2020051429 W TR2020051429 W TR 2020051429W WO 2022146242 A1 WO2022146242 A1 WO 2022146242A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
nanoparticle
blood culture
production method
cellulose
Prior art date
Application number
PCT/TR2020/051429
Other languages
English (en)
Inventor
Kürşat Seyfi DEMİREZEN
Original Assignee
Nanobi̇otech Arge İnovasyon Sağlik Ürünleri̇ Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanobi̇otech Arge İnovasyon Sağlik Ürünleri̇ Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇ filed Critical Nanobi̇otech Arge İnovasyon Sağlik Ürünleri̇ Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇
Priority to PCT/TR2020/051429 priority Critical patent/WO2022146242A1/fr
Publication of WO2022146242A1 publication Critical patent/WO2022146242A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L1/00—Compositions of cellulose, modified cellulose or cellulose derivatives
    • C08L1/02—Cellulose; Modified cellulose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/26—Infectious diseases, e.g. generalised sepsis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles

Definitions

  • the invention relates to a composition of a nanoparticle loaded bio adsorbent blood culture and the production method of the said composition for use in the health sector in defining the microbiological etiology in suspected infection cases.
  • Sepsis is a fatal infectious disease that involves many systems, especially causing hemodynamic changes, leading to shock, organ dysfunction, and organ failure.
  • the survival rate of the patients who have bacteria in their blood and treated with antibiotics within the first 2 hours is 80 %, while in patients who started antibiotics after 12 hours, this rate drops by almost half.
  • the pathogen detection rate in blood cultures has been increased with new methods. Examples of these are the development of new mediums, the addition of growth factors to the mediums, and the neutralization of growth inhibitors I metabolic products I antibiotic residues.
  • the average positivity period of automated blood culture measurement systems and bottles is 5 - 7 days, and for other systems, the average is 15 hours.
  • the value of conventional blood cultures is limited in slow or difficult breeds or in species that cannot be cultured and its sensitivity decreases in those who take antibiotic treatment and when the burden of microorganisms is low.
  • the false - positive blood cultures cause results to be interpreted with error, resulting in the incorrect use of antibiotics, additional laboratory tests, prolonged hospital stay and increased examination and treatment costs.
  • Sepsis patients are given high doses of antibiotics.
  • the blood taken from the patient is kept in a medium containing the nutrients of the pathogens that cause the infection, and pathogen growth is observed.
  • the blood taken from the patient due to antibiotic treatment can suppress these pathogens for a period of time, and the amount of growth appears to be lower than it should be, or there are certain deviations.
  • the BACTEC blood culture system (BD Diagnostics) produced for this purpose consists of an antibiotic binding resin on small glass beads, while the BacT / Alert blood culture system (BioMerieux Inc.) uses activated charcoal powder.
  • it is aimed to retain the antibiotic with the material chosen as adsorbent.
  • a patent application document numbered US4543328A is found.
  • the said document describes a process and device for the detection of pathogens, such as bacteria, fungi, and viruses, in blood in the presence of an anticoagulant agent.
  • the polymer is selected from the group of polyacrylate, polymethacrylate, polyhydroxy ethyl methacrylate, an adsorber resin, synthetic crosslinked polystyrene, cellulose acetate, collodion, and nylon.
  • the present invention relates to a composition of a nanoparticle loaded bio adsorbent blood culture and its production method that meets the above - mentioned requirements, eliminates all disadvantages, and brings some additional advantages.
  • the main purpose of the invention is to develop a new blood culture composition, especially for use in the examinations of sepsis patients.
  • the quality of the examination is improved, and the duration of the examination is shortened.
  • early precautions can be taken by obtaining rapid results in the treatment of fatal sepsis patients.
  • the blood culture composition of the invention contains bioadsorbent material charged with iron nanoparticles obtained through herbal synthesis.
  • a mixture comprising of the fruit and seeds of carob bean tree (Ceratonia siliqua), shell and resin of the olibanum (Boswellia carterii), as well as cellulose and flower - shaped iron hybrid nanostructure are used.
  • hydrosol obtained from the selected plant combinations as the reducing material is sufficient. It was determined that the composition containing the flower - shaped iron hybrid nanoparticle obtained using plant hydrosol not only retains the antibiotic in the environment but breaks it down, disrupting its structure.
  • Another object of the invention is to demonstrate that there is no need to use chemicals as reducers and stabilizers as in the current technique since the use of plant hydrosols is sufficient to obtain nanoparticles.
  • composition of the invention all reducing, stabilizing agents are composed of plant - derived raw materials.
  • biomaterial synthesis nano iron solution due to the herbal synthesis nano iron solution, the antibiotic adhesion surface area, and the rate of degradation of the antibiotic are increased.
  • the resin used in state of the art has a narrower surface area than nanoparticles, so it reaches saturation in a shorter time, and its performance decreases.
  • the antibiotics, which cannot be retained by resin give misleading results in diagnosis. Since the antibiotic in the environment is not only retained but also degraded employing the invention, the aforementioned problems can be avoided.
  • the invention comprises flower - shaped iron hybrid nanostructure and cellulose obtained using the fruit and seeds of carob bean tree and shell and resin of the Olibanum.
  • the cellulose charged with flower - shaped iron hybrid nanoparticles synthesized by herbal methods in the scope of the invention can be easily applied to any environment in which human contact is made and is environmentally friendly.
  • the innovative aspects of the product of the invention are listed below;
  • the antibiotic is adsorbed, and together with the deterioration of its structure, its effect is completely eliminated
  • Figure 1 shows The SEM image of plant synthesis flower - shaped iron hybrid nanostructure.
  • Figure 2 shows The TEM image of iron nanoparticles that form the center of the flower - shaped hybrid nanostructure.
  • Figure 3 shows the results of EDX analysis.
  • Figure 4 shows the results of FT - IR analysis.
  • Figure 5 shows the results of BET surface area analysis.
  • the invention relates to the composition of a nanoparticle - charged bioadsorbent blood culture and the production method of the said composition for use in determining the state of antibiotic administration.
  • the said blood culture composition comprises the fruit and seeds of carob bean tree and shell and resin of the Olibanum, iron, and cellulose.
  • Olibanum (Boswellia carterii) resin contains ester and acidic components and has high antimicrobial properties. In the composition of the invention, it is combined with carob bean tree hydrosols in the appropriate proportion to the composition, providing a flower - shaped organic - inorganic hybrid structure.
  • the carob bean tree (Ceratonia siliqua L.) plant contains a high percentage of polyphenols in its fruit.
  • the polyphenol group consists of carbohydrates (48 - 56 %), condensate tannin (16 - 20 %).
  • Polyphenols can reduce metals by forming complexes with metal ions.
  • Olibanum hydrosols in the appropriate proportion to the composition, providing a flower - shaped organic - inorganic hybrid structure.
  • composition of the invention in its most basic form, comprises the following steps;
  • the structure of the nanoparticle loaded bio adsorbent blood culture composition of the invention comprises, by weight
  • blood culture composition preferably comprises, by weight
  • the resulting iron nanoparticle loaded cellulose - based adsorbent material is added to existing blood culture bottles in the appropriate proportion instead of resin or activated carbon.
  • the invention is a blood culture composition containing carob bean tree and olibanum plant hydrosols, the iron, and cellulose.
  • the invention is a blood culture composition production method, characterized by comprising the following process steps of i. Preparation of the PBS solution ii. Obtaining of hydrosols of the fruit and seeds of carob bean tree, shell and resin of the olibanum in the PBS, iii. Thawing of iron, iv. Combining iron thawed in step (iii) and plant hydrosols treated with PBS in step (ii), v. Extraction of cellulose from sunflower heart and stem pulp, vi. Evaporation of the water of the mixture obtained in the process step (iii), vii. Charging the powdered iron nanoparticle obtained in the process step (vi) into the cellulose adsorbent obtained in the process step (iv).

Abstract

L'invention concerne une composition d'hémoculture contenant des fruits et des graines de caroubier, de la résine et de la coque d'oliban, des nanoparticules de fer et de la cellulose, et le procédé de production de ladite composition, destinée à être utilisée pour déterminer l'état de l'administration d'antibiotiques dans le traitement de la septicémie dans le secteur de la santé.
PCT/TR2020/051429 2020-12-29 2020-12-29 Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production WO2022146242A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/TR2020/051429 WO2022146242A1 (fr) 2020-12-29 2020-12-29 Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/TR2020/051429 WO2022146242A1 (fr) 2020-12-29 2020-12-29 Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production

Publications (1)

Publication Number Publication Date
WO2022146242A1 true WO2022146242A1 (fr) 2022-07-07

Family

ID=82259598

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2020/051429 WO2022146242A1 (fr) 2020-12-29 2020-12-29 Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production

Country Status (1)

Country Link
WO (1) WO2022146242A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4543328A (en) * 1978-06-16 1985-09-24 Boehringer Mannheim Gmbh Process for detecting pathogens
CN104928240A (zh) * 2015-07-01 2015-09-23 浙江元太生物科技有限公司 一种自体或异体红细胞培养基的制备方法
CN106479931A (zh) * 2016-11-04 2017-03-08 哈尔滨亿隆科技有限公司 一种血琼脂培养基的改良配方

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4543328A (en) * 1978-06-16 1985-09-24 Boehringer Mannheim Gmbh Process for detecting pathogens
CN104928240A (zh) * 2015-07-01 2015-09-23 浙江元太生物科技有限公司 一种自体或异体红细胞培养基的制备方法
CN106479931A (zh) * 2016-11-04 2017-03-08 哈尔滨亿隆科技有限公司 一种血琼脂培养基的改良配方

Similar Documents

Publication Publication Date Title
Ji et al. Enhanced eradication of bacterial/fungi biofilms by glucose oxidase-modified magnetic nanoparticles as a potential treatment for persistent endodontic infections
Ma et al. Preparation of chitosan–nylon-6 blended membranes containing silver ions as antibacterial materials
CN107997179B (zh) 一种乳酸菌微胶囊的制备方法
CN102266754B (zh) 一种活性炭-柿单宁复合吸附剂的制备方法
Sizentsov et al. Assessment of biotoxicity of Cu nanoparticles with respect to probiotic strains of microorganisms and representatives of the normal flora of the intestine of broiler chickens
Bing et al. Hydrogen-producing hyperthermophilic bacteria synthesized size-controllable fine gold nanoparticles with excellence for eradicating biofilm and antibacterial applications
CN112957457B (zh) 一种促进糖尿病伤口愈合的级联类酶纳米系统及其制备方法和应用
Kubyshkin et al. Antimicrobial effects of silver nanoparticles stabilized in solution by sodium alginate
Ghorbani et al. One-pot microbial approach to synthesize carbon dots from baker's yeast-derived compounds for the preparation of antimicrobial membrane
Zhang et al. Homology of selenium (Se) and tellurium (Te) endow the functional similarity of Se-doped and Te-doped mesoporous bioactive glass nanoparticles in bone tissue engineering
US10154628B2 (en) Coating metal oxide particles
WO2022146242A1 (fr) Composition d'une hémoculture bioadsorbante chargée de nanoparticules et son procédé de production
Yin et al. Preparation of amyloid immuno-nanoparticles as potential MRI contrast agents for Alzheimer’s disease diagnosis
Pender et al. Bactericidal activity of starch-encapsulated gold nanoparticles
CN110575816B (zh) 一种高吸附、高耐受重金属铅的植物乳杆菌yw11
He et al. Green Synthesis of MOF-Mediated pH-Sensitive Nanomaterial AgNPs@ ZIF-8 and Its Application in Improving the Antibacterial Performance of AgNPs
Hrebenyk et al. ZnS quantum dots encapculated with alginate: Synthesis and antibacterial properties
CN110604822A (zh) 一种磁性抗菌纳米系统及其制备方法
TWI600758B (zh) 具免疫調節機能的植物乳桿菌及其用途
AU2021103328A4 (en) Synthesis and pharmacological evaluation of ibuprofen entrapped silver nanoparticles using herbals
CN117210020A (zh) 一种鱼精蛋白与镍铁改性的UiO-66-NH2的杂化材料及其制备方法和应用
CN117137891A (zh) 一种原儿茶醛与镍铁改性的UiO-66-NH2的杂化材料及其制备方法和应用
CN108743533B (zh) 一种脂质体包封的钌多吡啶配合物及其应用
Wang et al. Facile synthesis of silver nanoparticles and their antimicrobial activity against several representative microbial species
Baqer et al. Anti Biofilm of Biosynthesized Silver Nanoparticles Mediated by Lactobacillus AcidophilusAgainst klebsiellapneumoniae

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 20968121

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 20968121

Country of ref document: EP

Kind code of ref document: A1