WO2022130732A1

WO2022130732A1 - Hygiene product derived from seaweeds and extract thereof, for oral cavity, nostrils, and throat

Info

Publication number: WO2022130732A1
Application number: PCT/JP2021/036078
Authority: WO
Inventors: 正一中村
Original assignee: 正一中村; 日本エー・シー・ピー株式会社
Priority date: 2020-12-15
Filing date: 2021-09-30
Publication date: 2022-06-23

Abstract

Provided is a hygiene product derived from seaweeds and an extract thereof, for oral cavity, nostrils, or throat.　This hygiene product for the oral cavity or nostrils includes a mouth washing liquid and a cleaning agent for the oral cavity, nasal cavity, or throat. The hygiene product is derived from seaweeds such as kelp, wakame, and green laver and/or an extract thereof, and has a deactivating action against viruses including SARS-CoV-2, influenza virus, and norovirus, and also has antibacterial action against bacteria including Escherichia coli and Staphylococcus aureus. Here, the hygiene product for oral cavity includes candies, chewing gums, and general chewing foods that are held in one's mouth for at least a certain period of time and facilitates saliva secretion.

Description

Hygiene products for the oral cavity, nostrils or throat with seaweed and its extracts

INDUSTRIAL APPLICABILITY The present invention has the effect of inactivating viruses and killing bacteria by using seaweeds and / or extracted components thereof, which are natural foods that have been eaten and ingested by humans for many years and whose safety has been confirmed. (Including paste, liquid or powder), tongue polish, mouthwash, dental rinse, mouthwash such as mouthwash, and oral, nasal or throat hygiene, oral cavity, throat, or Sanitary products containing spray liquid for the nasal cavity, and foods such as throat candy and chewing gum that stay in the oral cavity for a certain period of time and help maintain the hygiene of the oral cavity and throat such as teeth, gingiva, tongue, and throat. Regarding.

Here, the present invention is a sanitary product for maintaining the health and hygiene of the oral cavity, nasal passage or throat, and treats a vaccine or a bactericidal agent that inactivates a specific virus, or a specific disease. Not a remedy or medicine for.

The human mouth (oral cavity) is the digestive system that first takes food into the body when ingesting food and drink, and is therefore the first place to be eroded by foreign substances such as fungi, bacteria, and viruses from the outside world. ..

In addition, the nose and throat are the respiratory organs that first take breathing air (oxygen) into the body (lungs) and are formed integrally with the oral cavity, and the nose and throat are the airways (tracheal) and lungs (lungs). It is connected to the respiratory organs of the vesicle). Therefore, like the oral cavity, it is a place that is first easily eroded by foreign substances such as fungi and viruses from the outside world.

Humans digest food and drink taken from the oral cavity in the stomach, absorb the nutrients of the food digested in the stomach mainly in the small intestine, and absorb water in the large intestine. The nutrients and water taken into the body in this way are oxidized in various parts of the body by combining with oxygen taken in from the throat organs (nose, mouth, throat) and the respiratory organs connected to them (airway and lungs). As a result, the activity energy of the living body is generated, and the human body organs such as meat and bone are formed.

Humans are infected with respiratory diseases such as pneumonia and bronchitis due to the attachment of the causative virus or bacteria to the mucous membrane of the throat such as the nose, throat or trachea by breathing.

In addition, humans develop digestive disorders such as food poisoning in the digestive system because viruses such as norovirus and fungi that have entered foods and beverages are taken up by the digestive organs in the body from the oral cavity via the esophagus. Occur.

Thus, respiratory and digestive disorders caused by viruses and bacteria may rarely pass through mucous membranes such as the skin or eyes, but most of them are with the throat (mouth, throat and trachea) for breathing. , It results from entering the body via the oral cavity (lips, mouth, teeth, tongue, esophagus) that takes in food and drink.

By the way, the mouth is the trachea at the beginning of the digestive system of humans and animals, and is used exclusively for the intake of nutrients and oxygen. Many animals have tongues, teeth, and exocrine organs that are ancillary organs of the digestive organs, and they are used not only as food aids for chewing by teeth but also as a means to counter foreign enemies and protect themselves. ..

Many vertebrates, including humans, have masticatory organs equivalent to teeth attached to their oral cavity, and in addition to the functions of mastication and agitation with digestive juice (saliva) associated with eating, they assist in taste and vocalization, and breathe. It is an organ that assists various actions such as. In addition, only the outer lip and the opening / closing part may be referred to as the mouth.

Morphologically, the mouth refers to an opening that opens and closes on the anterior surface of the face with the assistance of the temporomandibular joint, and the inner surface of the mouth is covered with mucous membranes and is the open end of the digestive organs with various appendages.

The teeth chew as part of the digestion of food, attack the outside, and grasp things. The tongue not only controls the taste, but also agitates the food that has entered the oral cavity. Here, the salivary glands include a large number of salivary glands such as the submandibular gland, the parotid gland, and the sublingual gland, and secrete saliva as an aid to digestion.

The oral mucosa is a part of the digestive mucosa and is also a taste aid. Since the mucous membrane easily absorbs chemical substances with a smaller molecular weight than the outer skin, it can be said that it also plays a role as an absorptive organ because food tends to stay in the oral cavity.

In this way, the mouth is an entrance to the digestive system and an oxygen intake, and is one of the extremely important holes opened on the surface of the body. Around it, muscles for taking in (swallowing) food are developed. In addition, an organ for taking in food and cutting it is attached to the surrounding area. Their shape also varies greatly depending on the type of food taken in.

Food poisoning caused by epidemic influenza, parainfluenza, norovirus, etc., Middle East respiratory syndrome (MERS), viral acute bronchitis and pneumonia, measles, and more recently, a new coronavirus disease that was pandemicly certified by WHO in the spring of 2020. Diseases caused by virus infection such as the new corona virus (SARS-CoV-2) that causes (COVID-19) are mainly caused by droplets discharged from the trachea and air by a person infected with the virus by coughing or squeezing. It is transmitted from person to person or from animal to person through fine particles containing floating virus, virus attached to handles such as train leather, viruses attached to familiar items, and fungi and bacteria infected with these viruses. Infect by doing.

It has also been confirmed that many viruses and bacteria, including the new coronavirus, are also present in the saliva of infected persons.

As will be described later, although saliva is recognized to have antiviral and bactericidal effects, the amount of saliva produced per day decreases with age, so older people become more susceptible to viruses and bacteria. It is easy to get infected, and if it is infected, it is easy to get serious.

In addition, when the virus adheres to an article or the surface of the human body in a cold environment (low temperature and low humidity), it survives for a relatively long time without dying, especially for lowering the temperature of the respiratory organs including the human throat. Due to the dryness of the upper airway inner wall and the decrease in fiber activity (upper respiratory tract barrier function), human-to-human transmission is more widespread than in warmer environments.

For patients infected with these viruses, as therapeutic agents, for example, Tamiflu as a therapeutic agent for influenza, Remdecibel (generic name: Bekulley) and favipiravir (generic name: Avigan) as therapeutic agents for Ebola hemorrhagic fever and new coronavirus. Is used, and clinical trials of its therapeutic effect are underway. However, these therapeutic agents have a risk of causing side effects on pregnant women and idiosyncratic drugs, and considering the risk of the emergence of resistant viruses, first of all, they should not be infected and should not be infected by others. is important.

Therefore, from the viewpoint of preventing virus infection, it is effective to wear a mask to reduce the range of virus droplets from coughing or sneezing into the air as much as possible, and to reduce the amount of virus droplets into the outside atmosphere. Has been proven in use cases and various experiments around the world. It goes without saying that frequent hand washing and gargling are also important.

Furthermore, it is also important to frequently disinfect human fingers and familiar objects that people touch with various disinfectants that are effective in killing viruses and bacteria such as alcohol and sodium hypochlorite water. ..

However, in general, these disinfectants and bactericides have a tissue-damaging effect on the skin and mucous membranes, and there are certain restrictions on their use. There are clear restrictions on the use of each virus disinfectant, especially in the use of direct contact with the skin and mucous membrane surfaces. In particular, infants are liable to cause skin irritation and inflammation when they come into contact with alcohol or sodium hypochlorite water, and there is a risk of exacerbating atopic dermatitis or the like, especially for allergic patients.

In this way, in order to prevent the spread of virus infection from person to person, first of all, in order to prevent the virus from being transmitted from person to person, first of all, wearing a mask, frequent hand washing and gargle, and using a disinfectant solution such as alcohol are used for fingers and people. It is important to disinfect items that may come into contact.

By the way, not only in Japan but also in other countries around the world, when multiple people are placed in an environment or situation where virus infection can occur, one person is infected with the virus, while the other people are infected with the virus. Events that did not occur are seen in many cases. For example, even in the case of epidemic influenza infection, such an epidemic has occurred even once for decades among the large number of people who commute to work by train, which is usually crowded regardless of whether or not the influenza vaccine is inoculated. Some people are not infected with the flu, while others are infected with the flu almost every year.

In addition, regarding the new coronavirus disease (COVID-19), which became a worldwide pandemic infection in 2020, for example, in an extremely narrow enclosed space, a number of other people including one or several infected persons who are not infected Even if you talk, sing, or eat and drink loudly for a long time in a dense and close environment, not everyone who was there will not be infected with the new coronavirus, or even if it is infected, the new coronavirus will not be infected. There have been many reports of cases in which the disease does not develop due to the disease.

The reasons why individuals who are placed in the same environment are infected or not infected with the virus are their past history of virus infection of the same or similar type, and immunity to certain viruses. There may be differences in the presence or absence of antibodies, constitution, or individual health and nutritional status, but the amount of saliva produced and the ciliary activity (upper airway barrier function) that eliminates viruses and bacteria that have entered the upper airways of humans. It is presumed that the difference in) also has an effect.

In the case of the new coronavirus, at present, for example, the number of people infected with the coronavirus and the number of deaths of Japanese (including people in East Asia such as China, South Korea, and Taiwan) are in other developed countries in terms of population ratio. It is also a fact that it is considerably lower than that of Western countries.

The reasons for the large difference in the number of infected people and the number of deaths in the population ratio are the difference in social habits such as shaking hands and hugging in daily life in Europe and the United States, and the normal time during the virus infection period of low temperature and low humidity. It has been pointed out that there is a feeling of resistance to wearing a mask from the virus, and that there is a difference in the strength of social norm awareness. Even after the stage where eating in restaurants was banned and masks were enforced, the spread of the new coronavirus continues to be extremely serious in terms of population ratio compared to Japan.

On the other hand, as will be described later, in the long history of human beings, it has a bactericidal and inactivating action against viruses and fungi, and even if it is eaten or brought into contact with the mucous membrane in the oral cavity, it has been used for many years. There are foods and their extracts whose safety has been confirmed.

For this reason, the inventor of the present application has a large number of people in East Asia, including Japanese, on a daily basis, as one of the reasons why such a difference occurs in the infection level of viruses and the like. Focusing on foods and foods that Western countries do not usually eat, select multiple such foods, and in each food, anti-virus effect that prevents human virus infection, virus A test was conducted to confirm the inactivating effect and the bactericidal effect of Escherichia coli and Yellow staphylococcus.

By the way, as a conventional example in which the virus inactivating effect due to such a food-containing element has been confirmed, from a group consisting of proteoglycan, mucopolysaccharide (for example, glycosaminoglycan, etc.), and a sugar chain-peptide isolate of proteoglycan. At least one selected has a virus inactivating effect, and further, a proteoglycan-containing extract obtained from Amefurashi and Namako, particularly from Namako, and a sugar chain-peptide isolate of proteoglycan in the extract. , Documents showing that it has a high virus inactivating effect are disclosed (see Patent Document 1).

In addition, it has long been reported that some menthol and herbs have antiviral effects, and examples of such herbs include rosehip, thyme, and echinacea, which are rich in vitamin C. Has been done.

JP-A-2019-19085

However, nameko mushrooms and Amefurashi, which are disclosed in Patent Document 1 and have a virus inactivating effect, and extracts from these are not foodstuffs that Japanese people and the like eat on a daily basis. In addition, these foods and their processed products are expensive, and even if these foods have a high virus-inactivating effect, it is difficult for many people to eat them on a daily basis.

In addition, the hearsay that vitamin C and some herbs have antiviral effects has not been clearly confirmed by scientific clinical trials, or studies that deny or question their physiological effects. Many reports have been confirmed.

The present invention does not exert an immune effect only against a specific virus unlike a vaccine, and unlike a vaccine, there is no risk of causing serious side effects to some people, and the present invention is routinely used. A naturally occurring virus inactivating agent that inactivates a virus, which should be made less susceptible to infection by various viruses by eating it or ingesting its components, or should be prevented from becoming serious even if infected with a virus. It is an object of the present invention to provide an oral, nasal or throat hygiene product containing the virus.

By the way, respiratory and digestive disorders caused by viruses and bacteria are all, first, the throat for breathing (mouth, throat and trachea) or the oral cavity (lips, mouth, teeth, tongue, esophagus) that takes in food and drink. ), The present invention inactivates viruses including the new coronavirus "SARS-CoV-2" that has entered from the outside world and kills bacteria in the oral cavity and throat. It is intended to provide sanitary products for the oral cavity, nasal passages or throat.

The present invention further promotes good saliva secretion, activates ciliary activity to eliminate viruses and bacteria that have entered the larynx and upper respiratory tract, enhances the upper respiratory tract barrier function, and confirms safety. Provides oral and nasal hygiene products that have a virus inactivating effect and a bacterial killing effect by a combination of natural foods or extracts thereof and specific additives whose safety has been confirmed for a long time. The purpose is to do.

There are various particularly important issues to be fulfilled by the sanitary products according to the present invention, but they are summarized in the achievement of the following objectives (1) to (4). That is,
(1) Not a vaccine that inactivates a specific virus or a drug that kills a specific bacterium, but an inactivation of various viruses and sterilization of various bacteria.
(2) It is not a therapeutic drug or drug for treating a specific disease, and there is no concern that it will cause any health safety even if it is ingested / taken for a long period of time. In addition, there is no risk of developing resistant viruses and resistant bacteria.
(3) Even if the seaweed and its extract according to the present invention are ingested or taken in contact with sensitive mucous membranes in the human body such as the throat, nostrils, oral cavity, or respiratory organs such as the bronchi, pain, pain, It does not cause discomfort, and there are no problems with the texture, taste, and smell.

The applicant of the present application submits the seaweeds according to the present invention and / or extraction thereof prior to carrying out various 1st to 8th detailed effect confirmation tests described in detail in the specification of the present application over a long period of time. Even if an object touches the sensitive throat, nostrils, oral cavity, bronchi, etc. of the human body, it does not cause pain, pain, or discomfort, and there is no problem in texture, taste, and smell. be.

As a result of this prior confirmation test, the applicant of the present application crushed the seaweed and / or its extract according to the present invention into a size of about 5 μm extremely finely, and powder of this ultrafine size was subjected to physiological saline (physiological saline (). It was confirmed that if it is dissolved in cold water and boiling water and taken or ingested, it can be ingested or taken without any discomfort as a sanitary product for the oral cavity, throat, and nose. Therefore, in the 1st to 8th effect confirmation tests described later, the seaweed and / or its extract according to the invention is pulverized to a size of approximately 5 μm extremely finely, and this ultrafine size powder is physiologically used. It was dissolved in saline solution (cold water and boiling water).

The physiological saline solution is an aqueous solution of sodium chloride (NaCl) prepared to have almost the same osmotic pressure as human body fluid. In the Japanese Pharmacopoeia and prescription drugs, a saline solution containing 0.9 w / v% of sodium chloride is defined as "physiological saline solution".

Based on these conditions, the inventor and applicant of the present application focus on a plurality of natural foods that many Western countries have few opportunities to eat from among the foods and foods that Japanese people eat on a daily basis. The test of virus inactivation and bactericidal action on the selected various foodstuffs was actually confirmed by several tests over a long period of time.

As a result, seaweeds such as kelp, wakame seaweed, and sea lettuce or extracts of such seaweeds have a remarkable reduction effect and virus inactivation effect, and a remarkable test effect on killing bacteria such as Escherichia coli and Staphylococcus aureus. confirmed.

Therefore, the present invention relates to seaweeds such as kelp, wakame, and aosa and their extracts or extracts of seaweeds such as kelp, wakame, and aosa (in the present application, "seaweeds such as kelp, wakame, and aosa and / or". It has an inactivating effect on viruses including SARS-CoV-2, which is a new type of coronavirus consisting of "the extract"), and a bactericidal effect on bacteria including Escherichia coli and staphylococcus aureus. It is intended to provide oral or nasal hygiene products containing cleaning agents for oral or throat.

Here, the mouthwash includes a paste-like, liquid or powdery dentifrice, mouthwash, dental rinse, and mouthwash. In addition, the oral cleaning agent contains an oral spray solution. In addition, the cleaning agent for the nostrils includes nasal drops and nasal spray liquid. In addition, the throat cleanser comprises a throat spray solution.

These oral cleaning agents, mouthwashes such as dental rinses and mouthwashes, and oral, nasal or throat cleaning agents are used for a while (usually about 10 seconds to 1 minute) in the oral cavity. Since it stays in the nasal cavity and throat, it promotes saliva secretion and, as described later, enhances the effect of inactivating action against the virus by interacting with saliva. Further, since the hygienic product according to the present invention has a strong bactericidal action against bacteria including Escherichia coli and Staphylococcus aureus according to the test described later, a virus that cannot grow its own cells by itself is transmitted through the bacteria. It effectively prevents the virus from infecting the human body.

Therefore, the invention of the present application includes a throat lozenge, chewing gum, and a pacifier food. Similar to the above-mentioned oral hygiene products, these foods stay in the mouth for a certain period of time to promote saliva secretion, and the solution mixed with saliva comes into contact with the trachea and esophagus for a certain period of time. , Inactivates viruses present in the mucous membranes of the esophagus and kills bacteria. Moreover, since it is a food, even if it penetrates into the mucous membrane of the human body or is digested and absorbed, there is no risk of causing an adverse effect on the human body like chemicals and disinfectants.

By the way, the extract of the seaweed in the present invention contains fucoidan, laminarin, arginine, alginic acid (sodium alginate), mannuronic acid, gluronic acid, or iodine, which are viscous polysaccharides that were later revealed in the results of several experiments. Any or a combination thereof.

The extracts of these seaweeds were confirmed to have a virus inactivating effect and a bactericidal effect containing the new coronavirus (SARS-CoV-2) by the results of several rigorous tests by the applicant of the present application, which will be described later. be.

Fucoidan, laminarin, arginine, sodium alginate, mannuronic acid and glulonic acid are stored polysaccharides contained in seaweeds and mushrooms and are easily extracted by water, many of which are viscous components and have long been anti-antibacterial. It is known to have tumor action, antithrombotic action, and antihypertensive action. It was confirmed by this test that these extracts also have a virus inactivating effect and a bactericidal effect in addition to these conventionally known effects.

Further, it is preferable to add either or both of citric acid and baking soda to the hygienic product of the present invention. Citric acid and baking soda are nutrients whose safety has been confirmed, and their refreshing acidity and carbon dioxide gas generating action promote saliva secretion and have an antibacterial action in themselves. Furthermore, the refreshing acidity and mild stimulation of citric acid and baking soda activate the mucous membranes and cilia that cover the inner wall of the airway from the human throat to the lungs.

In addition, saliva itself has antiviral activity, which is that bound sialic acid in saliva causes the virus to reach receptors on the cell surface of the throat and upper respiratory tract. It is presumed that the infection is prevented by adsorbing. That is, since virus infection is suppressed according to the amount of bound sialic acid in sublingual saliva, ingestion of citric acid and baking soda is a kelp or seaweed and seaweed extract belonging to the brown algae according to the present invention. Combined with the products extracted from, it suppresses the infectivity of the virus.

In addition, when an appropriate amount of citric acid or baking soda is ingested, a large amount of sublingual saliva secreted from the tongue and jaw is secreted out of saliva secreted from multiple locations such as sublingual saliva, sublingual saliva, and submandibular saliva. The results of the test have been published, and it is known that this sublingual saliva has a higher antiviral effect, virus inactivating effect, and bactericidal effect than other saliva.

By the way, in the present invention, aminolevulinic acid and / and L-glutamic acid are added to the hygiene products. Here, aminolevulinic acid is a starting material of the porphyrin synthesis pathway and is a substance used in plastids of prokaryotes and eukaryotes. Recently, a virus caused by 5-aminolevulinic acid (5-ALA) has been introduced. As a result of the cultured cell experiment using the virus, the virus infection suppressing effect was confirmed. In the present invention, a more remarkable virus infection suppressing effect was confirmed by the combined use with seaweed and its extract.

Furthermore, various tests conducted by the applicant of the present application confirmed that better test results were obtained when polyphenols were added to the hygienic products according to the present invention. Here, polyphenols are components of bitterness and pigments present in most plants, and generally, like vitamin C and vitamin E, have a strong antioxidant effect and are harmless to harmful substances such as active oxygen. It is known to be useful for the prevention of lifestyle-related diseases such as the action of changing vitamins and arteriosclerosis. In the present invention, a remarkable virus infection suppressing effect was confirmed by the combined use with seaweed and its extract.

Further, in the present invention, it was confirmed that alcohol (red wine) for drinking was used in combination with seaweed and its extract in the hygiene product to suppress virus infection. Since red wine contains polyphenols and alcohol and the secretion of saliva is promoted by a slight acidity, it is understood that it is one of the best foods and drinks for inactivating the virus.

Further, in the present invention, β-carotene (β-carotene) may be added to the hygiene product. Since β-carotene is converted to vitamin A and acts, it maintains the health of skin and mucous membranes in vivo and contributes to the proliferation and differentiation of mucosal cells. In addition, β-carotene has been reported to have antioxidant and immunostimulatory effects. In the present invention, it was confirmed that alcohol for drinking was used in combination with seaweed and its extract in the hygienic product to suppress virus infection.

As described above, the sanitary products according to the present invention are all natural or naturally derived foodstuffs, their constituent elements and safe materials for additives, and various kinds including SARS-CoV-2 are subjected to a strict confirmation test described later. It was confirmed that the virus has an inactivating effect on the virus and a bactericidal effect on bacteria including Escherichia coli and Staphylococcus aureus.

Here, what is the extract of seaweed? It is produced by dissolving dry powder of seaweed in a saline solution or a physiological saline solution, and the salt weight ratio in the saline solution or the physiological saline solution is 0.9 to 3.4%.

By the way, the air that humans breathe through their mouths and noses enters the lungs via the throat and trachea, and the air that is inhaled in this way is foreign matter such as dust, soot, mold, bacteria, and viruses. It is known that the mucous membrane covering the inner wall of the airway and the action of the cilia protect the air from foreign matter invading the trachea and reaching the lungs.

Furthermore, if the sanitary products for the oral cavity, nostrils and throat according to the present invention are ingested and kept in the oral cavity for a certain period of time, the periodontal bacteria in the oral cavity can be reduced.

By the way, periodontal disease is a bacterial infection in which periodontal disease bacteria in periodontal plaque (plaque) inflame the hug and destroy surrounding tissues. In recent years, diabetes, brain disease, and heart disease A research paper has been published that contributes to this. In addition, it has been reported that a large amount of periodontal bacteria was recently detected in the mouths of many people who died from infection with the new coronavirus, and many of the severely ill people infected with the new coronavirus are serious. It has been reported that people are infected with periodontal disease, and it has long been reported that the incidence of influenza is reduced to about one-tenth by reducing oral bacteria by proper brushing and brushing of the tongue.

From many such cases, it is possible that a virus that does not have the ability to self-proliferate enters the gene into bacteria such as periodontal bacteria, and the bacteria that have virus cells grow in the human mouth and mucous membranes. It is speculated.

Therefore, in order not to be infected with viruses such as SARS-CoV-2 and various influenza, saliva is secreted well, bacteria in the oral cavity, throat, and nasal cavity are reduced to maintain good hygiene. This is very important.

According to the present invention, it does not cause side effects like a vaccine, and it does not have an infection-preventing effect against only a specific virus like a vaccine, but by taking or ingesting it on a daily basis without difficulty. Hygiene for the oral cavity, nasal cavity or throat that makes it difficult to be infected with various viruses including SARS-CoV-2 or does not worsen the symptoms even if infected, and also has a sterilizing or bactericidal effect on Escherichia coli and yellow staphylococcus. It made it possible to provide supplies.

The test result (1) of the effect on PED virus which is a coronavirus similar to the form of SARS-CoV-2 by the hygiene product for oral cavity, nostril or throat according to the present invention is shown. Regarding the seaweed kelp, which is a sanitary product for the oral cavity, nose, or throat according to the present invention, each of the main constituents of the seaweed, alginic acid, fucoidan, and iodine, and further, alginic acid + fucoidan + The test result (2) of the effect on the new coronavirus "SARS-CoV-2" with respect to the mixed component of iodine is shown. The test result (3) of the effect on PED virus which added alcohol to the hygiene product of this invention is shown. Regarding the seaweed kelp and wakame seaweed, which are the sanitary products of the present invention, each of iodine, arginine, and fucoidan, which are constituents of seaweed, and the mixed component of iodine + arginine + fucoidan, against PED virus. The test result (4) of the effect is shown. The test result (5) of the bactericidal effect on Escherichia coli by the hygiene product of this invention is shown. The test result (5) of the bactericidal effect against Staphylococcus aureus by the sanitary product of the present invention is shown. For each of the elements or additives constituting the sanitary products of the present invention, L-glutamic acid, β-carotene (β-carotene), sea lettuce, red wine (containing 14% alcohol and polyphenols), laminarin and fucoidan, against PED virus. The test result (6) of the effect is shown. The test result (7) of the action effect with the hygiene product of this invention against influenza virus is shown. The test result (8) of the effect on SARS-CoV-2 which is a new type coronavirus by the hygiene product for oral cavity, nostril or throat according to the present invention is shown.

The antiviral agent or virus inactivating agent contained in oral hygiene products and food products according to the present invention is derived from kelp or seaweed belonging to brown algae and products extracted from seaweed extract.

Therefore, before explaining in detail the embodiment for carrying out the invention, kelp and its components will be briefly described.

Kelp belongs to seaweed, and seaweed is a general term for algae that grow in the sea. Unlike seagrass, algae do not bloom and grow offspring by spores. Most seaweeds are edible.

Seaweed is classified into blue algae, diatoms, green algae, brown algae, red algae, etc. according to color, and kelp belongs to brown algae. However, the difference in the color of kelp depends on the water depth of the place where seaweed grows, that is, the amount of sunlight that reaches, and the shallow water is green, the deep place is brown, and the place where the light is most difficult to reach is red. Many of the brown algae to which kelp belongs include wakame seaweed, hijiki seaweed, mozuku seaweed, and mekabu. Green laver belongs to green algae, and gliopeltis and amanori belong to red algae.

There are 45 types of seaweed brown algae in 14 genera confirmed in Japan. Of these, kelp, which is a cold current brown algae, is distributed in the Pacific coast north of Miyagi prefecture and the sea throughout Hokkaido, and Hokkaido is the main production area. Most of the domestic production of kelp is taken from Hokkaido.

Kelp contains arginine, alginic acid, and fucoidan, a unique viscous polysaccharide that exists only in seaweeds. Here, arginine is known to have an effect of lowering blood pressure, and fucoidan is known to have an effect of preventing thrombosis and cancer.

Fucoidan is a type of sulfated polysaccharide. It is a dietary fiber that is abundant in the mucilage of brown algae such as kelp, wakame seaweed (including mekabu, which is a part of it), and mozuku. Similar substances have also been found in animals such as sea cucumbers having an antiviral activity described in Patent Document 1 described above.

Alginic acid, alginic acid, and fucoidan contained in kelp are compounds in which tens to hundreds of thousands of L-fucose (polysaccharides) are linked by A1-2 and A1-4 bonds. The average molecular weight is about 200,000. Fucoidan is classified into U-fucoidan containing glucuronic acid, F-fucoidan consisting only of sulfated fucose, and G-fucoidan containing galactose.

Here, unlike mushrooms (Agaricus, etc.) and other polysaccharide (sugar chain) components, L-fucose has a sulfate group bonded to fucose. This L-fucose is abundantly contained in brown algae (Mozuku, Mekabu, Kombu, Akamoku, Sargassum fulvelus such as Sargassum thunbergii, etc.) and is often expressed as a sticky component of seaweed.

On the other hand, arginine is an acidic polysaccharide composed of two types of uronic acid called mannuronic acid (M) and gluronic acid (G). Since arginine greatly changes in hardness and elasticity depending on the ratio of (M) and (G) bound, it can be used as a material for pudding, jelly, ice cream, jam, etc., for emulsification purposes such as yogurt, cheese, etc. It is used for various purposes such as artificial ice cream, and in kelp, it is combined with calcium and magnesium and exists in a jelly state.

Here, arginine is known to have the following physiological actions (1) to (3).
(1) Action to lower blood pressure Excessive intake of salt upsets the balance between sodium and potassium ions in the blood and causes blood vessels to constrict, resulting in an increase in blood pressure. In particular, when "arginine", which is bound to potassium among arginine, is taken into the body together with food, arginine separates potassium by gastric acid. After that, it moves to the intestine and binds to the sodium ions contained in the food taken with it and carries it out of the body. On the other hand, potassium separated from arginine becomes potassium ions and is absorbed from the intestine, and has the function of expelling sodium in the blood. In this way, arginine has a dual function of lowering blood pressure.
(2) Action to activate the action of digestive enzymes When arginine is ingested with food, it promotes digestion by enhancing the actions of amylase and protease in the intestine, which are digestive enzymes.
(3) Action to remove harmful substances Accumulation of harmful substances and pollutants in the body causes various abnormalities and diseases. In an experiment in which arginine was given to an experimental animal contaminated with radioactive strontium, it has been reported that it has a function of excreting the radioactive element from the body.

By the way, kelp is rich in dietary fiber, and the main components of the dietary fiber are arginine and fucoidan, which have a different chemical structure from the dietary fiber contained in vegetables and grains.

As mentioned above, the slime component of kelp contains these two polysaccharides. In kelp distributed as a dry matter, amino acids forming umami and mannitol having a sweet taste are present as components other than dietary fiber. Furthermore, minerals such as magnesium and calcium, and iodine are also contained as nutritional components, making it an excellent food that meets the purpose of nutritional supplementation.

The slip component "fucoidan" contained in the kelp seaweed and the like according to the present invention is said to have a large number of biological activities. For example, anticoagulant action, cell adhesion inhibitory action, anti-inflammatory action, cell protection from viral infection, antitumor action.

In addition, iodine is a mineral that is essential for the human body and is mainly contained in kelp, etc., and has been used as the main component of mouthwash for a long time to reduce inflammation in the back of the oral cavity and trachea. Has been done. However, it is said that the amount of iodine required for the human body is 0.095 to 0.13 mg per day (kelp: equivalent to 40 to 60 mg), so if you take this on a daily basis, do not exceed this usage limit. It is also necessary to consider.

It is said that fucoidan, a water-soluble dietary fiber contained in kelp, slows the movement of food ingested into the body from the stomach to the small intestine. Fucoidan uses sulfate groups to irritate the gastric mucosa in the stomach where digestion has slowed down.

The antiviral agent or the virus inactivating agent containing the product extracted from the kelp or seaweed and the seaweed extract according to the present invention may be a part of the action of such fucoidan.

That is, fucoidan and its components contained in kelp may contribute to the enhancement of the defense power of immune cells throughout the body by stimulating the mucosal immune function in the intestine. For example, fucoidan held by M cells existing in the human body. When lymphocytes (NK cells, T cells, B cells) attack, NK cells, one of the lymphocytes involved in immunity, are activated, and when the activated lymphocytes get on the blood flow. The secretion of antibodies progresses. As a result, immunity is enhanced.

Further, in the present invention, it is preferable to add citric acid to seaweeds and seaweed extracts to produce them. Here, citric acid is an organic compound contained in citrus fruits and the like, and is one of hydroxy acids. Since it has a refreshing acidity, it is often used as a food additive, so there is no problem with its safety.

Citric acid is also one of the factors that inhibit glycolytic phosphofructokinase activity and regulate the influx from glycolysis into the citric acid cycle, and is indirectly intramuscular lactic acid in the citric acid cycle. It is said that it also has the effect of reducing fatigue after exercise from the point of decomposing. The reason is that citric acid also forms a chelate complex with calcium, which is one of the fatigue substances, and the binding with calcium is generally predominant in the trade-off with the decrease in acidosis in lactic acid decomposition. It can be effective in reducing fatigue.

Approximately 1000 to 1500 mL of saliva is secreted daily, depending on the age. It has also been confirmed that the older the elderly, the lower the amount of saliva secreted. For this reason, older people are more likely to be infected with viruses and fungi, and when infected, they are more likely to become severely infected.

Therefore, what is extremely important in relation to the present invention is that the acidity of citric acid promotes saliva secretion when it is eaten.

Most of the saliva water is secreted by the parotid and submandibular glands, but saliva derived from the minor salivary glands (palatal gland, tongue gland, cheek gland, lip gland, molar tooth gland, and Ebnel gland) is added to this. At rest, saliva from the submandibular gland is 60-70%. Submandibular gland / sublingual gland The saliva drainage part is the lower part of the tongue (oral floor).

On the other hand, when citric acid or the like is eaten, saliva secretion is promoted due to its taste-stimulating effect. It has been confirmed that saliva has functions such as digestive action, oral self-cleaning action, oral mucosa protective action, pH regulation, antibacterial action, and antiviral action. In this way, the large amount of saliva excreted by citric acid and the antiviral effect of saliva suppress the prolongation of viral load and infectivity.

Further, in the present invention, it is preferable to add baking soda to seaweed and seaweed extract to produce the product. Here, as described above, baking soda is a highly safe food additive that has been conventionally eaten as a baking powder as a weakly alkaline food material for inflating cookies, pancakes, and the like.

And, the baking soda made based on the provisions of the Food Sanitation Law may be used as an antacid for excess gastric acid as a medicine. Since the gastric fluid contains hydrochloric acid, sodium hydrogen carbonate, which constitutes baking soda, decomposes to generate carbon dioxide bubbles, which stimulate the taste and stomach and promote the secretion of further saliva and gastric fluid. To do.

I. [Confirmation test (1)]
From the above point of view, the applicant of the present application uses an external testing institution to first form SARS-CoV-2 with seaweeds constituting the oral, nostril or throat sanitary products according to the present invention. The inactivating effect on PED virus, which is the closest coronavirus to PED virus, was tested (1).

In the following, I. [Confirmation test (1)] to VIII. [Confirmation test (8)], which are sequentially described in the present application, were conducted by the applicant of the present application on behalf of an external authoritative testing institution. In the present application, reports of the first to eighth test results from the testing institution are posted without any operation or evaluation on the test results. However, since the virus reduction number of the test material showing the confirmation test result is described in a logarithmic display of 10, the logarithmic display value is quantified in decimal to make the value easier to understand. Was added.

(A) Test material: Seaweed and seaweed extract Test material I: Seaweed and seaweed extract were dissolved in physiological saline to prepare a 2% solution.

Test material II: Seaweed and seaweed extract were dissolved in boiling water (100 ° C) physiological saline to a concentration of 2%, and used after cooling. Here, a sterile phosphate buffer was used as a control material.

(B) Test Microorganism As the test microorganism (virus), a P-5V strain of PED virus (Porcine epidemic diarrhea virus) was used.

This PED virus (Porcine epidemic dialrrhea virus) is commonly called a pig infectious disease virus, and the pathogen PED virus belongs to the genus Alphacoronavirus of the coronavirus family and is radial (crown-shaped) on the surface of the envelope. That is, it has a corona-like protruding spike, and its genome is a plus single-stranded RNA. It is the type of coronavirus that most closely resembles the pandemic-infected new coronavirus (COVID-19) in 2020.

In the virus belonging to the genus Alphacoronavirus of the coronavirus family, the tip of a corona-shaped spike that protrudes radially on the surface of this envelope attaches to the surface of the cell membrane that constitutes the human body, and the virus enters the human body. As a result, it is scientifically known that the virus is infected with the virus.

Therefore, in the above-mentioned confirmation test of the present application, the inactivating action against the PED virus belonging to the genus Alphacoronavirus of the coronaviridae family was confirmed in the virus inactivation confirmation test of the present application. It is presumed and expected that it is highly possible that (COVID-19) can also exert its inactivating action.

Vero cells were used as cultured cells for PED virus (Porcine epidemic dialrrhea virus). These Vero cells are derived from the kidney epithelial cells of African green monkeys and are a cell line used for cell culture. It is one of the most commonly used cell lines along with Hela Cell.

(C) Setting of group As a test group, 0.1 mL of virus solution was added to 1 mL of the above-mentioned test material (2% solution of seaweed and seaweed extract in physiological saline), and the sensitization time was set. , 1 minute after the start of the test.

As a control group, 0.1 mL of virus solution was simply added to 1 mL of phosphate buffer without adding the above-mentioned test materials, and the sensitization time was set to 0 minutes and 1 minute after the start of the test.

(D) Test method The test method was carried out with reference to "Virus Experiments General Remarks Revised 2nd Edition Maruzen Co., Ltd. Virus Neutralization Test Method".

(E) Test procedure (E-1) Preliminary test:
Prior to the test, the effect of the test material on cultured cells (cytotoxicity) was investigated.

After diluting the test material 10-fold with phosphate buffer, the cultured cells were inoculated, the maximum concentration indicating the normal state of the cultured cells was confirmed, and the virus concentration used for the test was determined. As a result, regarding cytotoxicity, poor cell growth was confirmed in the test material 100-fold solution. Therefore, it was found that in the test, it is necessary to dilute the mixed solution of the test material and the virus solution 100 times or more and then inoculate the cells. Therefore, the virus addition concentration was set to ¹⁰⁶ TCID ₅₀ / mL or more.

(E-2) Mixing of main test and test solution According to the test category, 1 mL each of the test material and the phosphate buffer was separated, and the virus solution was added to the concentration determined in the preliminary test.

After adding the virus solution, the mixture was allowed to stand at room temperature (25 ° C) for a predetermined time.

(E-3) Main test / Cell inoculation and bacterial count measurement The mixed solution after sensitization was diluted 10-fold in each of the above test categories, and 100 μL of each cell cultured on a 96-well plate was inoculated.

Judgment is made by culturing at 37 ° C in carbon dioxide gas culture (5%) for 5 days, then observing the cultured cells under a microscope, confirming the presence or absence of viral proliferation by CPE (cell degeneration) appearing in the cultured cells, and calculating the concentration. did.

(F) Results FIG. 1 shows the test results (1) of the effects of the oral, nostril, or throat sanitary products according to the present invention on the PED virus, which is a coronavirus similar to the morphology of SARS-CoV-2. Table 1 shown is a summary of the test results.

It should be noted that the vertical axis of FIG. 1 also has the vertical axis of 10 as an exponential notation in the subsequent FIGS. 2 to 7, which means that each time one frame is lowered, it becomes one tenth.

In the control group (comparative test group without test materials), no change in viral load was observed between the start of the test and 1 minute after the start of the test (10 ^6.1 TCID ₅₀ / mL).

On the other hand, in the test group, <10 ^3.5 TCID ₅₀ / mL (below the detection limit: decreased by 99.7% or more) 1 minute after the start. This result of "below the detection limit: 99.7% or more reduction" is a value showing a surprisingly high virus inactivating effect.

(G) Discussion This time, the inactivating effect test of the test material against PED virus (Porcine epidemic diarrhea virus), which is usually called porcine epidemic diarrhea virus, was carried out. As a result, it was found that the PED virus had an astonishing virus inactivating effect of 99.7% or more in a reaction of 1 minute after contact.

As mentioned above, the genome of the PED virus (Porcine epidemic dialrrhea virus) is a plus single-stranded RNA, which is the type of coronavirus that most closely resembles the new coronavirus (COVID-19). It was speculated that the inactivating effect on "SARS-CoV-2" was also large.

II. [Confirmation test (2)]
Therefore, the applicant of the present application confirmed the remarkable antiviral effect and virus inactivating effect of the seaweed according to the sanitary product of the present invention against the above-mentioned PED virus. A confirmation test of the effect on "2" was conducted. The details are described below.

(A) Test material: Seaweed and seaweed extract Test material 1: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution Test material 2: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution (prepared by boiling water dissolution)
Test material 3: 2% arginine powder sodium chloride aqueous solution Test material 4: 2% fucoidan powder sodium chloride aqueous solution (prepared to dissolve in boiling water)
Test material 5: 2% iodine powder physiological salt aqueous solution (prepared to dissolve in boiling water)
Test material 6: 2% (arginine + fucoidan + iodine) powder physiological saline solution A sterile phosphate buffer solution was used as a control material.

(B) Test microorganism As the test microorganism (virus), SARS-CoV-02 (new coronavirus) was used. This SARS-CoV-02 is a human-derived isolate, and after isolation and culture using vero cells from saliva, confirmation of amplification of the SARS-CoV-2 gene using real-time PCR (Ministry of Health, Labor and Welfare notification method). It is a virus strain that performed.

The cultured cells, vero cells, are cell lines derived from the kidney epithelium of African green monkeys.

Setting of group (C) As a control group, 0.1 mL of virus solution was added to 1 mL of phosphate buffer, and the sensitization time was set to 0 seconds and 60 seconds after the start of the test.

As a test group, 0.1 mL of virus solution was added to 1 mL of the above test material, and the sensitization time was set to 15 seconds, 30 seconds, and 60 seconds after the start of the test.

After diluting the test material 10-fold with phosphate buffer, the cultured cells were inoculated, the maximum concentration indicating the normal state of the cultured cells was confirmed, and the virus concentration used for the test was determined.

As a result, the cytotoxicity was as shown in the following table, and it was confirmed that the cells grew poorly in the test material 10 times solution at the maximum. Therefore, it was found that in the test, it is necessary to dilute the mixed solution of the test material and the virus solution 10 times or more and then inoculate the cells. The virus addition concentration was ¹⁰⁶ TCID ₅₀ / mL or more.

After adding the virus solution, the mixture was allowed to stand at room temperature (25 ° C.) for a predetermined time.

(E-3) Main test / Cell inoculation and bacterial count measurement The mixed solution after sensitization was diluted 10-fold in each test category, and 100 μL of each cell cultured on a 96-well plate was inoculated.

Judgment was made by culturing at 37 ° C. in carbon dioxide gas culture (5%) for 5 days, then observing the cultured cells under a microscope, confirming the presence or absence of viral proliferation by CPE (cell degeneration) appearing in the cultured cells, and calculating the concentration. ..

(F) Results FIG. 2 shows the seaweed kelp, which is a sanitary product for the oral cavity, nose, or throat according to the present invention, and each of the main constituents of the seaweed, alginic acid, fucoidan, and iodine. Furthermore, the test results of the effect of the mixed component of alginic acid + fucoidan + iodine on the new coronavirus "SARS-CoV-2" are shown. In addition, the details of the test results shown in FIG. 2 are summarized in Tables 2A, 2B and 2C. Note that Table 2C is an easy-to-understand version of Table 2B.

In the control group, no change in viral load was observed between the start of the test and 60 seconds after the start of the test (10 ^6.5 TCID ₅₀ / mL).

On the other hand, in Test Group 1, 99.7% in 15 seconds and 99.9% in 60 seconds, in Test Group 2, 99.7% in 15 seconds, 99.9% in 60 seconds, and in Test Group 3, 15 seconds. 59.3% in 60 seconds, 93.7% in 60 seconds, 97.5% in 15 seconds, 99.7% in 60 seconds, 90.0% in 15 seconds, 99 in 60 seconds in test plot 5. In the test group 6, the virus infectivity decreased by 9.0%, 98.4% in 15 seconds, and 99.8% in 60 seconds.

The virus infection rate for SARS-CoV-02 described above is generally close to the detection limit, and this test confirmed a surprisingly high virus inactivating effect.

Moreover, since both seaweed and seaweed extract are 2% solutions and the particle size of kelp powder is 5 μm, the present invention can be used for mouthwash, oral cavity, nasal cavity or throat. It was found that even if it is applied to sanitary products containing the cleaning agent, it can be used without discomfort.

(G) Consideration This time, the inactivation effect test of the test material on SARS-CoV-2 was carried out. As a result, it was found that the contact for a maximum of 60 seconds had an inactivating effect of 93.7 to 99.9%.

III. [Confirmation test (3)]
Furthermore, the applicant of the present application conducted a confirmation test of the effect on the PED virus in which alcohol was added to the hygienic product of the present invention. The details are described below.

(A) Test material: Seaweed and seaweed extract Test material 1: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution Test material 2: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution (prepared by boiling water dissolution)
Test material 3: 1% kelp powder Particle size 5 μm Physiological salt aqueous solution (prepared to dissolve in boiling water)
Test material 4: 0.5% kelp powder particle size 5 μm physiological salt aqueous solution (prepared to dissolve in boiling water)
Test material 5: Alcohol content 25 degrees barley shochu diluted 3 times with purified water (prepared to dissolve in boiling water)
Test material 6: Alcohol content 25 degrees 5% kelp shochu diluted 3 times with purified water (prepared to dissolve in boiling water)
A sterile phosphate buffer was used as a control material.

The cultured cells are vero cells (cells derived from the kidney epithelium of African green monkeys).

Setting of group (C) As the test group, 0.1 mL of virus solution was added to 1 mL of the above-mentioned test material, and the sensitization time was set to 15 seconds, 30 seconds, and 60 seconds after the start of the test.

As a control group, 0.1 mL of virus solution was added to 1 mL of phosphate buffer, and the sensitization time was set to 0 seconds and 60 seconds after the start of the test.

(F) Results The test results for PED virus are shown in FIG. 3, Table 3A, Table 3B and Table 3C. In addition, Table 3C makes it easy to understand the display of Table 3B.

In the control group, no change in viral load was observed between the start of the test and 60 seconds after the start of the test (10 ^6.7 TCID ₅₀ / mL).

On the other hand, in Test Group 1, 99.7% in 15 seconds and 99.9% in 60 seconds, in Test Group 2, 99.7% in 15 seconds, 99.9% in 60 seconds, and in Test Group 3, 60 seconds. 99.8% in Test Group 4, 97.4% in 60 seconds in Test Group 4, 93.6% in 60 seconds in Test Group 5, and 97.4% in Test Group 6 60 seconds after the start. rice field.

(G) Consideration This time, the inactivation effect test of the test material against PED virus (pig-infected coronavirus) was carried out. As a result, it was found that the contact for a maximum of 60 seconds had an inactivating effect of 93.6 to 99.9%.

IV. [Confirmation test (4)]
Furthermore, the applicant of the present application applies to the seaweed kelp and wakame seaweed, which are the sanitary products of the present invention, and also for iodine, arginine, and fucoidan, which are the main constituents of the seaweed, and further, iodine + arginine. A confirmation test of the effect on PED virus was conducted on the mixed component of + fucoidan. The details are described below.

(A) Test material: Seaweed and seaweed extract Test material 1: 2% kelp powder physiological salt aqueous solution Test material 2: 2% kelp powder physiological salt aqueous solution (prepared by dissolving in boiling water)
Test material 3: 2% kelp powder aqueous solution Test material 4: 2% wakame powder physiological salt aqueous solution Test material 5: 2% iodine powder physiological salt aqueous solution Test material 6: 2% arginine powder physiological salt aqueous solution Test material 7: 2% fucoidan powder Physiological salt aqueous solution Test material 8: 2% (iodine + fucoidan + arginine powder) Physiological salt aqueous solution A sterile phosphate buffer was used as a control material.

(B) Test Microorganism As the test microorganism (virus), a P-5V strain of PED virus (Porcine epidemic diarrhea virus) (porcine epidemic diarrhea virus) was used.

As a control group, 0.1 mL of virus solution was added to 1 mL of phosphate buffer, and the sensitization time was set to 0 seconds, 15 seconds, 30 seconds, and 60 seconds after the start of the test.

As a result, the cytotoxicity is as shown in the following table. It was confirmed that the cells grew poorly even in the test material 100 times solution at the maximum. Therefore, it was found that in the test, it is necessary to dilute the mixed solution of the test material and the virus solution 100 times or more and then inoculate the cells. The virus addition concentration was ¹⁰⁶ TCID ₅₀ / mL or more.

(F) Results The test results for PED virus are shown in FIG. 4, Table 4A, Table 4B and Table 4C. Note that Table 4C is a table that makes Table 4B easier to understand.

On the other hand, in test group 1, 99.8% in 30 seconds after the start, 99.9% or more in 30 seconds after the start in test group 2, 99.7% in 60 seconds after the start of test in test group 3, and 99.7% in test group 4. 98.4% 60 seconds after the start, 98.4% 60 seconds after the start of the test in test group 5, 59.3% 60 seconds after the start in test group 6, 99 60 seconds after the start of the test in test group 7. In the test group 8, the virus infectivity decreased by 9.0% and 99.5% in 60 seconds after the start.

(G) Consideration This time, the inactivation effect test of the test material against PED virus (pig-infected coronavirus) was carried out. As a result, it was found that the contact for a maximum of 60 seconds had an inactivating effect of 59.3 to 99.9%.

V. [Confirmation test (5)]
Furthermore, the applicant of the present application conducted a confirmation test of the bactericidal effect against Escherichia coli and Staphylococcus aureus by the sanitary product of the present invention. The details are described below.

(A) Test material: Seaweed and seaweed extract Test material 1: 2% kelp powder physiological salt aqueous solution Test material 2: 2% kelp powder physiological salt aqueous solution (prepared by dissolving in boiling water)
Sterilized saline was used as a control material.

(B) Test microorganisms As the test microorganisms, Escherichia coli ATCC117775 and Staphylococcus aureus ATCC6538 were used.

The above microorganisms were pre-cultured in nutrient medium and prepared with sterile purified water to a concentration of about ¹⁰⁸ CFU / mL, which was used as a test bacterial solution.

Setting of group (C) As the test group, 0.1 mL of the test bacterial solution was added to 10 mL of the control material, and the sensitization time was set to 30 seconds and 60 seconds after the start of the test.

As a control group, 0.1 mL of the test bacterial solution was added to 10 mL of the test material, and the sensitization time was set to 0 seconds, 30 seconds, and 60 seconds after the start of the test.

(D) The test method was carried out with reference to "JIS Z 2801 (antibacterial processed product-antibacterial test method / bactericidal effect)" and the Phenol coefficient method.

(E) Test procedure (E-1) Microbial test method (measurement of bacterial count in test solution)
The test solution was appropriately diluted with sterile physiological saline and cultured in nutrient agar medium and each selective medium (Escherichia coli: desoxycholate agar medium and Staphylococcus aureus: egg yolk-added mannit salt medium). The culture was carried out under aerobic conditions at 35 ° C. for 24 to 48 hours, and the populations that grew after the culture were counted to obtain the number of the bacteria.

(E-2) Test method The test material and the control material were placed in a sterilized test tube, 0.1 mL of the test bacterial solution was added to 10 mL of the material, and the mixture was well mixed.

According to the test settings, immediately after mixing and after reacting at room temperature for a certain period of time, the number of remaining viable bacteria was measured according to the microbiological test method.

(F) Result [E. coli]
The test results are shown in Table 5A.
For the control group, the number was the same from the start to the end of the test, which was 640000 CFU / mL. 60 seconds after the start of the test, it was 360000 CFU / mL (43.7% decrease) in Test Group 1 and 320,000 CFU / mL (50.0% decrease) in Test Group 2.

[Staphylococcus aureus]
The test results are shown in Table 5B.
For the control group, the number was almost the same from the start to the end of the test, and it was 2300000 CFU / mL. 60 seconds after the start of the test, it was 1100000 CFU / mL (52.1% decrease) in Test Group 1 and 1600000 CFU / mL (30.4% decrease) in Test Group 2.

(G) Consideration As a result of the test, it was confirmed that the test material 1 decreased by 43.7% 60 seconds after contact and the test material 2 decreased by 50.0%. In addition, it was confirmed that the number of Staphylococcus aureus decreased by 52.1% in the test material 1 60 seconds after contact and by 30.4% in the test material 2.

VI. [Confirmation test (6)]
Furthermore, the applicant of the present application has applied the PED virus to each of the elements or additives constituting the sanitary products of the present invention, such as L-glutamic acid, β-carotene, red wine (containing 14% alcohol and polyphenols), laminarin and fucoidan. We conducted a confirmation test of the effect on. The details are described below.

(A) Test material: Seaweed, seaweed extract and additives Test material 1: 2% L-glutamic acid powder Physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 2: 2% β-carotene powder Physiological salt aqueous solution (prepared to dissolve in boiling water)
Test material 3: 2% sea lettuce fine particle powder physiological salt aqueous solution (prepared to dissolve in boiling water)
Test material 4: Alcohol content 14% red wine liquid Test material 5: 1% kelp 5 μm powder Alcohol content 14% red wine liquid (prepared to dissolve in boiling water)
Test material 6: 1% Laminarin powder Physiological salt aqueous solution (prepared to dissolve in boiling water)
Test material 7: Fucoidan aqueous solution A sterile phosphate buffer solution was used as a control material.

As a result, the cytotoxicity was as shown in Table 6A, and it was confirmed that the cells grew poorly in the test material 10 times solution at the maximum. Therefore, it was found that in the test, it is necessary to dilute the mixed solution of the test material and the virus solution 10 times or more and then inoculate the cells. The virus addition concentration was ¹⁰⁶ TCID ₅₀ / mL or more.

(E-2) Mixing of main test and test solution According to the test category, 1 mL each of the test material and the phosphate buffer was separated, and the virus solution was added to the concentration determined in the preliminary test. After adding the virus solution, the mixture was allowed to stand at room temperature (25 ° C.) for a predetermined time.

(E-3) Main test / cell inoculation The sensitized mixed solution was diluted 10-fold in each test category, and 100 μL of each cell cultured on a 96-well plate was inoculated.

(F) Results The above test results are shown in FIG. 6, Table 6A, Table 6B and Table 6C. Note that Table 6C is a table that makes Table 6B easier to understand.

In test plot 1, 37.5% in 15 seconds and 90.0% in 60 seconds, in test plot 2, 75.3% in 15 seconds, 90.0% in 60 seconds, and in test plot 3, 59 in 15 seconds. .3%, 93.7% in 60 seconds, 37.5% in 15 seconds, 99.7% in 60 seconds, 99.7% in 15 seconds, 99.9% in 60 seconds in test plot 4. In Test Group 6, the virus infectivity decreased by 95.9% in 15 seconds and 99.3% in 60 seconds, and in Test Group 7, 98.4% in 15 seconds and 99.8% in 60 seconds.

(G) Consideration This time, the inactivation effect test of the test material against PED virus (pig-infected coronavirus) was carried out. As a result, it was found that the contact for a maximum of 60 seconds had an inactivating effect of 90.0 to 99.9%.

VII. [Confirmation test (7)]
Furthermore, the applicant of the present application conducted a confirmation test of the action and effect of the hygienic product of the present invention on the following influenza viruses. The details are described below.

(A) Test material: Seaweed and seaweed extract Test material 1: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution Test material 2: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution (prepared by boiling water dissolution)
A sterile phosphate buffer was used as a control material.

(B) Test microorganism As the test microorganism (virus), influenza virus (swine influenza virus H1N1 IOWA strain) was used.

The cultured cells are MDCK cells (dog kidney-derived cell lines).

As a result, regarding cytotoxicity, it was confirmed that the cells did not grow well in the 10-fold solution of any of the test materials. Therefore, it was found that in the test, it is necessary to dilute the mixed solution of the test material and the virus solution 10 times or more and then inoculate the cells. The virus addition concentration was ¹⁰⁶ TCID ₅₀ / mL or more.

Judgment was made by culturing at 37 ° C. in carbon dioxide gas culture (5%) for 5 days, collecting the culture supernatant in each well, confirming the presence or absence of virus growth by hemagglutination reaction, and calculating the concentration.

(F) Results The test results are shown in FIGS. 7, 7A and 7B.

In the control group, no change in viral load was observed between the start of the test and 60 seconds after the start of the test (10 ^6.9 TCID ₅₀ / mL).

In Test Group 1, the virus infectivity decreased by 99.3% in 15 seconds and 99.9% in 60 seconds, and in Test Group 2, 99.3% in 15 seconds and 99.9% in 60 seconds.

(G) Consideration This time, the test of the inactivating effect of the test material against influenza virus was conducted. As a result, it was found that the contact for 15 to 60 seconds had an inactivating effect of 99.3 to 99.9%.

VIII. [Confirmation test (8)]
Based on the results of the above-mentioned [confirmation test (1)] to [confirmation test (7)], the applicant of the present application further inactivates the virus containing SARS-CoV-2 consisting of kelp and its extract. In order to confirm in more detail, a confirmation test (8) was conducted.

FIG. 8 shows the test result (8) of the effect of the hygienic product for oral cavity, nostril or throat according to the present invention on SARS-CoV-2, which is a new type of coronavirus.

(A) Test material: Seaweed and seaweed extract Test material 1: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution Test material 2: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution Test material 3: 2% kelp powder particle diameter 5 μm physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 4: 2% kelp powder particle size 5 μm physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 5: 2% kelp powder Particle size 5 μm Physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 6: 2% kelp powder particle size 5 μm physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 7: 2% kelp powder Particle size 5 μm Physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 8: 2% laminarin powder physiological sodium chloride solution Test material 9: 2% phloroglucinol powder physiological salt aqueous solution (preparation of boiling aqueous solution)
Test material 10: 2% Ecklonia stolon powder Physiological salt aqueous solution (prepared to dissolve in boiling water)
A sterile phosphate buffer solution was used as a control material.

By the way, the above-mentioned "test material 1", "test material 2", and "test material 3"-"test material 7" are the same, but this confirms the variation in the effect in the same test state, and this test. I am doing it to know the reliability of the result.

(B) Test microorganism As the test microorganism (virus), SARS-CoV-02 (new coronavirus) was used. This SARS-CoV-02 is a human-derived isolate, and after isolation and culture using vero cells from saliva, confirmation of amplification of the SARS-CoV-2 gene using real-time PCR (Ministry of Health, Labor and Welfare notification method). It is a virus strain that performed. The cultured cells used here are vero cells (cells derived from kidney epithelium of African green monkeys).

(F) Results The test results for the above SARS-CoV-02 are shown in FIG. 8, Table 8A, Table 8B, Table 8C and Table 8D. In addition, Table 8C and Table 8D are easy to understand Table 8B.

In the control group, no change in viral load was observed between the start of the test and 60 seconds after the start of the test (10 ^6.3 TCID ₅₀ / mL).

On the other hand, in Test Group 1, 99.3% in 15 seconds and 99.9% in 60 seconds, in Test Group 2, 99.6% in 15 seconds, 99.9% in 60 seconds, and in Test Group 3, 15 seconds. 99.7% in 60 seconds, 99.7% in 60 seconds, 99.3% in 15 seconds, 99.9% in 60 seconds, 99.6% in 15 seconds, 99 in 60 seconds in test plot 5. 9.9%, 99.3% in 15 seconds, 99.9% in 60 seconds in test plot 6, 99.6% in 15 seconds, 99.9% in 60 seconds, 15 seconds in test plot 8. 90.0% in 60 seconds, 96.0% in 60 seconds, 96.0% in 15 seconds in test plot 9, 98.4% in 60 seconds, 99.0% in 15 seconds, 99 in 60 seconds in test plot 10. The virus infection titer decreased by 0.6%.

Below, the detailed results of I. [Confirmation test (1)] to VIII. [Confirmation test (8)] described above are summarized in Tables 9 to 12D.

Table 9 summarizes the virus inactivating effects of each seaweed component.

As shown in Table 9, the main components constituting the seaweeds according to the present invention are the new coronavirus "SARS-CoV-2" that causes COVID-21 and its approximation to this "SARS-CoV-2". It was confirmed that it exerts a remarkable inactivating effect (decrease in the number of viruses) against the "PED virus". From this, it is expected that the same remarkable inactivating action will be exerted on the mutant of the new coronavirus "SARS-CoV-2".

Table 10 is a table summarizing the virus inactivating effect (virus reduction rate) by SARS-CoV-2, PEDV (PED virus), and influenza virus, and 2% kelp powder, physiological saline having a particle size of 5 μm. The number of tests was increased for physiological saline solution of boiling aqueous solution of 2% kelp powder particle diameter of 5 μm, which was the same as that of water, and the variation in the inactivation effect was confirmed.

As shown in Table 10, as in Table 10 above, the main components constituting the seaweeds according to the present invention are the new coronaviruses "SARS-CoV-2" and "influenza virus" that cause COVID-21. , It was confirmed that it exerts a remarkable inactivating effect (a drastic decrease in the number of viruses) against "PED virus" which is similar to "SARS-CoV-2". From this, it is easy to exert the same remarkable inactivating action against various types of "influenza virus" such as Hong Kong type and Soviet type, and various mutants of "SARS-CoV-2". is expected.

Table 11 is a table summarizing the inactivating effect of physiological saline containing 2% powder fine particles of a plurality of types of seaweed (kelp, seaweed, sea lettuce, Ecklonia stolon) against PED virus.

As shown in Table 11, it can exert a remarkable inactivating effect (dramatic decrease in the number of viruses) against the "PED virus" which is similar to the new coronavirus "SARS-CoV-2" that causes COVID-21. It was confirmed.

Table 12A is a table summarizing the inactivating effect of the seaweed powder according to the present invention on the "PED virus" which is similar to "SARS-CoV-2".

As shown in Table 12A, as in Table 10 above, the seaweed powder according to the present invention has a remarkable inactivating effect (virus) on the "PED virus" which is similar to "SARS-CoV-2". It was confirmed that the number was drastically reduced).

Table 12B shows "SARS-CoV-2" and "SARS-CoV-2", which are similar to the new coronaviruses "SARS-CoV-2" and "SARS-CoV-2", which cause COVID-21, which are the main components and various additives constituting the seaweeds according to the present invention. It is a table summarizing the inactivating effect on "PED virus".

As shown in Table 12B, the main components and various additives constituting the seaweed according to the present invention are the new coronaviruses "SARS-CoV-2" and "SARS-CoV-2" that cause COVID-21. It was confirmed that it had a remarkable inactivating effect (a drastic decrease in the number of viruses) against the similar "PED virus".

Table 12C is a table summarizing the inactivating effect of alcohol and liquor containing kelp components on "PED virus" which is similar to the new coronavirus "SARS-CoV-2" which causes COVID-21.

As shown in Table 12C, as is conventionally known, wine containing alcohol and kelp components is resistant to "PED virus" which is similar to the new coronavirus "SARS-CoV-2" which causes COVID-21. It was confirmed that it has a remarkable inactivating effect (a drastic decrease in the number of viruses).

Table 12D is a table summarizing the bactericidal effect results of the seaweed powder according to the present invention on Escherichia coli and Staphylococcus aureus.

As shown in Table 12D, it was confirmed that the seaweed powder according to the present invention has a remarkable bactericidal effect against Escherichia coli and Staphylococcus aureus. From this, it can be understood that the seaweed powder according to the present invention has a bactericidal effect against other bacteria that cause food poisoning.

Table 13 is similar to the new coronaviruses "SARS-CoV-2" and "SARS-CoV-2" that cause COVID-21, which are the main components and additives constituting the seaweeds according to the present invention. It is a table summarizing the inactivating effect (dramatic decrease in the number of viruses) against "PED virus".

As shown in Table 13, the main components and additives constituting the seaweed according to the present invention are the new coronaviruses "SARS-CoV-2" and "SARS-CoV-2" that cause COVID-21. It was confirmed that it exerts a remarkable inactivating effect (a drastic decrease in the number of viruses) against the "PED virus" which is close to the above.

In addition, Table 13 shows the "PED virus" which is similar to the new coronaviruses "SARS-CoV-2" and "SARS-CoV-2" that cause COVID-21 in the seaweed powder and its components according to the present invention. It is a table summarizing the inactivating effect on viruses such as "norovirus" and the bactericidal effect on "E. coli" and "yellow staphylococcus".

As shown in Table 13, the seaweed powder and its components according to the present invention are "PED viruses" similar to the new coronaviruses "SARS-CoV-2" and "SARS-CoV-2" that cause COVID-21. It was confirmed that it has a remarkable inactivating effect on viruses such as "Norovirus" and a bactericidal effect on "E. coli" and "Yellow staphylococcus".

(G) Consideration This time, the inactivation effect test of the test material on SARS-CoV-2 was carried out. As a result, it was found that the contact for a maximum of 60 seconds had an inactivating effect of 96.0 to 99.9%.

The antiviral agent or virus inactivating agent containing seaweeds such as kelp, wakame, and aosa according to the present invention or seaweeds extracted from seaweed powder and products extracted from seaweed extracts is a vaccine. It does not cause such side effects, and it does not have an infection-preventing effect only on a specific virus, but it is difficult to be infected with various viruses by eating it on a daily basis or ingesting an extract extracted from it. It is expected to inactivate the virus, which has the effect of not exacerbating the symptoms even if infected with the virus.

Further, what is important in relation to the seaweed and the seaweed extract according to the present invention is that the acidity action of citric acid promotes saliva secretion when it is eaten. In addition, when citric acid or the like is eaten, saliva secretion is promoted due to its taste-stimulating effect. The saliva exerts a digestive action, an oral self-cleaning action, an oral mucosa protective action, a pH regulation, an antibacterial action, and an antiviral action.

Further, in relation to the seaweed and the seaweed extract according to the present invention, baking soda is a food additive prepared in accordance with the provisions of the Food Sanitation Law, and as a pharmaceutical product, it is used as an antacid against excessive gastric acid. Sometimes used, it not only has bactericidal and antiviral effects in itself, but also promotes saliva production.

By the way, the seaweeds and seaweed extracts confirmed to have an inactivating effect on the above-mentioned corona-type virus were produced by dissolving them in saline solution or physiological saline solution (confirmed at a weight ratio of 0.9% in the test). Therefore, it is possible and desirable to use it on a daily basis as a food additive for adding a taste component to foods such as candy and kelp tea and cooked foods.

In addition, since kelp usually contains about 5% of fucoidan containing a mucous polysaccharide, influenza-specific secretion of fucoidan (F-type, U-type, G-type) into the trachea of the human body, which has been confirmed conventionally. It is also expected to promote the production of type Ig antibody.

In addition, iodine, which is abundant in kelp, is present in the thyroid gland in the human body and constitutes thyroid hormone. Thyroid hormones control physiological processes such as reproduction, growth, and development, and promote the development and growth of the brain, peripheral tissues, skeleton, etc., especially in fetuses and infants, during pregnancy. There is no problem even if a woman or an infant takes an appropriate amount, and it is desirable to take it positively.

Periodontal disease, which is said to be the cause of tooth loss, is one of the lifestyle-related diseases in parallel with the westernization of eating habits. Many inflammatory diseases are mainly caused by plaque, but they are caused by many complex factors, not just plaque. There are also many periodontal diseases that are completely unrelated to plaque (non-plaque). Furthermore, there are individual differences in causal factors, and the susceptibility and degree of progression of periodontal disease vary from person to person.

According to a report by the World Health Organization (WHO), about 38% of the world's total population is deficient in iodine. It is estimated that the Japanese ingest about 1.5 milligrams of iodine as an average daily intake, but no decrease in thyroid function or goiter due to iodine intake has been observed. ..

However, since it has been reported that thyroid poisoning occurred by ingesting 28 milligrams per day, mainly kelp soup stock, it is desirable to refrain from overdose over a long period of time. It has been reported that the upper limit should be .2 milligrams.

For the kelp extract, a leachate such as water (including salt solution) is added to finely chopped seaweed and its extract, and the mixture is left as it is for a certain period of time to leach various components contained in the kelp. Is concentrated or produced by reducing the pressure below 85 ° C, for example, so that the kelp is not filtered or the volatile components are not lost.

If the concentrated solution having a starch syrup-like concentration is added in the conventional manufacturing process of candy, throat candy, chewing gum, syrup kelp, troche, drinking water, etc., candy, chewing gum, syrup kelp as food can be added. It is possible to produce drinking water as food such as.

Similarly, the concentrated solution containing kelp extract can be used as a supplement such as tablets or capsules, as a quasi-drug, as a mouthwash or as a nasal wash.

In addition, the kelp extract can be used not only in the body but also as a mouthwash or nasal wash, disinfectant, and soaps (shampoo, bar soap, hand wash, etc.).

As described above, in the oral hygiene products or foods according to the present invention, for respiratory diseases and digestive diseases caused by viruses and bacteria, the throat for breathing (mouth, throat and trachea) and the oral cavity for taking in food and drink (lips, Since it enters the body via the mouth, teeth, tongue, and esophagus), viruses and bacteria that have entered from the outside world in the oral cavity and throat are extremely effective and do not cause any side effects on the human body. It can provide cavity hygiene products and food.

The hygienic product according to the present invention is not a vaccine that inactivates a specific virus or a drug that kills a specific bacterium, but can inactivate various viruses and sterilize various bacteria. I was able to confirm it.

The hygienic product according to the present invention is not a therapeutic drug or a drug for treating a specific disease, and is resistant to any health safety even if it is ingested / taken for a long period of time. There is no risk of developing viruses or resistant bacteria.

Further, of particular note is that the seaweed and its extract according to the present invention are ingested or taken in contact with sensitive mucous membranes in the human body such as the throat, nose, oral cavity, or respiratory organs such as bronchi. However, the seaweed and / or its extract according to the present invention is pulverized to a size of approximately 5 μm so as not to cause pain, distress, or discomfort and to have no problem in texture, taste, and smell. Moreover, in the effect confirmation test in which this ultrafine size powder was dissolved in physiological saline (cold water and boiling water), a remarkable inactivating action and bactericidal action of the virus could be confirmed.

Here, since the oral hygiene product or food according to the present invention is used so as to remain in the oral cavity for a certain period of time without causing discomfort to humans, not only Escherichia coli and Staphylococcus aureus other than viruses, but also dental caries bacteria. It can also be expected to have the effect of sterilizing periodontal bacteria.

Furthermore, periodontal disease bacteria in periodontal plaque (dental plaque) inflame the hug and destroy surrounding tissues. In recent years, it has contributed to diabetes, brain disease, and heart disease. A research paper has been published, and the oral hygiene products or foods according to the present invention can be expected to have these secondary effects.

Claims

Mouthwash, oral cavity, nasal cavity or throat, which has a bactericidal action against bacteria including Escherichia coli and Staphylococcus aureus, as well as an inactivating action against a virus consisting of seaweeds such as kelp, wakame and aosa and / or an extract thereof. Oral or nasal hygiene products containing mouthwashes.
The hygienic product according to claim 1, wherein the virus is contained in SARS-CoV-2.
The hygienic product according to claim 1, wherein the virus contains influenza virus and norovirus.
The hygiene product according to claim 2 or 3, wherein the oral hygiene product includes a throat lozenge, chewing gum, and a pacifier food that stay in the mouth for a certain period of time to promote saliva secretion.
The sanitary product according to claim 2 or 3, wherein the seaweed extract contains one or more of the viscous polysaccharides fucoidan, alginic acid, laminarin, mannuronic acid, gluronic acid, or iodine.
The hygienic product according to claim 5, wherein either or both of citric acid and baking soda are added to the hygienic product.
The hygienic product according to claim 5, wherein aminolevulinic acid is added to the hygienic product.
The hygienic product according to claim 5, wherein L-glutamic acid is added to the hygienic product.
The hygiene product according to claim 5, wherein polyphenol is added to the hygiene product.
The hygiene product according to claim 5, wherein alcohol for drinking is added to the hygiene product.
The hygiene product according to claim 5, wherein β-carotene is added to the hygiene product.
The sanitary product according to claim 5, wherein the seaweed extract is produced by dissolving dried seaweed powder in a saline solution or a physiological saline solution.
The sanitary product according to claim 12, wherein the dried seaweed powder has a particle size of approximately 5 μm.
The sanitary product according to claim 12 or 13, wherein the weight ratio of the dried seaweed powder to the saline solution or physiological saline solution is approximately 2%.