本发明的实施方式Embodiments of the present invention
以下结合具体实施例来进一步说明本发明,但实施例并不对本发明做任何形式的限定。除非特别说明,本发明采用的试剂、方法和设备为本技术领域常规试剂、方法和设备。The present invention is further described below in conjunction with specific embodiments, but the embodiments do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.
除非特别说明,以下实施例所用试剂和材料均为市购。Unless otherwise specified, the reagents and materials used in the following examples are commercially available.
实施例1
具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波Example 1
Shampoo with the function of treating pet ectoparasite skin diseases and antibacterial function
本实施例提供100 g具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波,由如下重量的各组分组成:The present embodiment provides 100 g of shampoo with the function of treating pet ectoparasite skin disease and antibacterial function, which is composed of the following components by weight:
非泼罗尼2 g、酮康唑1
g、苯扎溴铵1 g、烷基糖苷(APG)6 g、椰油酰胺丙基甜菜碱(CAB)25 g、十二烷基二甲基氧化胺6 g、鲸蜡硬脂基葡糖苷1 g、乙二胺四乙酸二钠(EDTA-2Na)0.1 g、氨基硅油微乳液0.1 g、山梨酸钾0.01 g、其余去离子水补齐。Fipronil 2 g, Ketoconazole 1
g, benzalkonium bromide 1 g, alkyl glycoside (APG) 6 g, cocamidopropyl betaine (CAB) 25 g, dodecyl dimethyl amine oxide 6 g, cetearyl glucoside 1 g, disodium ethylenediaminetetraacetate (EDTA-2Na) 0.1 g, amino silicone oil microemulsion 0.1 g, potassium sorbate 0.01 g, and the rest of the deionized water.
上述具有抗菌功能的动物香波的制备方法为:The preparation method of the above-mentioned animal shampoo with antibacterial function is:
(1)在烧杯中加入35mL的去离子水,并加热到60℃,然后将烷基糖苷、椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入非泼罗尼,待非泼罗尼完全溶解后加入酮康唑,保持在70℃加热下搅拌溶解,待酮康唑充分溶解后加入氨基硅油微乳液,待氨基硅油微乳液完全溶解后停止加热;(1) Add 35 mL of deionized water to the beaker and heat it to 60°C, then add the alkyl glycoside, cocamidopropyl betaine and dodecyl dimethyl amine oxide into the deionized water and stir to dissolve, completely. After dissolving, add fipronil, add ketoconazole after fipronil is completely dissolved, keep stirring at 70°C to dissolve, add amino silicone oil microemulsion after ketoconazole is fully dissolved, and wait until amino silicone oil microemulsion is completely Stop heating after dissolving;
(2)在步骤(1)冷却过程中,加入EDTA-2Na搅拌溶解,冷却至40℃时加入苯扎溴铵搅拌溶解,待苯扎溴铵完全溶解后加入鲸蜡硬脂基葡糖苷搅拌溶解,待鲸蜡硬脂基葡糖苷完全溶解后加入山梨酸钾和余量的去离子水,搅拌均匀后分装保存即可。(2) During the cooling process of step (1), add EDTA-2Na and stir to dissolve. When cooled to 40°C, add benzalkonium bromide and stir to dissolve. After benzalkonium bromide is completely dissolved, add cetearyl glucoside and stir to dissolve. , after the cetearyl glucoside is completely dissolved, add potassium sorbate and the remaining amount of deionized water, stir evenly, and then pack and store.
实施例2
具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波Example 2
Shampoo with the function of treating pet ectoparasite skin diseases and antibacterial function
本实施例提供100 g具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波,由如下重量份的组分组成:The present embodiment provides 100 g of shampoo with the function of treating pet ectoparasite skin disease and antibacterial function, which is made up of the following components by weight:
非泼罗尼1 g、硝酸咪康唑3
g、醋酸氯己定3 g、烷基糖苷(APG)15 g、椰油酰胺丙基甜菜碱(CAB)20 g、十二烷基二甲基氧化胺10g、脂肪聚乙二醇醚(VD-92)5 g、EDTA 1 g、水溶性羊毛脂1 g、苯甲酸钠0.125 g、其余去离子水补齐。Fipronil 1 g, Miconazole Nitrate 3
g, 3 g of chlorhexidine acetate, 15 g of alkyl glycosides (APG), 20 g of cocamidopropyl betaine (CAB), 10 g of dodecyl dimethyl amine oxide, fatty polyethylene glycol ether (VD -92) 5 g, 1 g of EDTA, 1 g of water-soluble lanolin, 0.125 g of sodium benzoate, and the rest of the deionized water.
上述具有抗菌功能的动物香波的制备方法为:The preparation method of the above-mentioned animal shampoo with antibacterial function is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到70℃,然后将烷基糖苷、椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入非泼罗尼,待非泼罗尼完全溶解后加入硝酸咪康唑,保持在70℃加热下搅拌溶解,待硝酸咪康唑充分溶解后加入水溶性羊毛脂,待水溶性羊毛脂完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, heat it to 70 °C, and then add alkyl glycosides, cocamidopropyl betaine and dodecyl dimethyl amine oxide to the beaker. Stir and dissolve in deionized water, add fipronil after complete dissolution, add miconazole nitrate after fipronil is completely dissolved, keep stirring and dissolve under heating at 70 °C, and add water-soluble after miconazole nitrate is fully dissolved Lanolin, stop heating after the water-soluble lanolin is completely dissolved;
(2)在步骤(1)冷却过程中,加入EDTA搅拌溶解,冷却至40℃时加入醋酸氯己定搅拌溶解,待醋酸氯己定完全溶解后加入脂肪聚乙二醇醚(VD-92)搅拌溶解,待脂肪聚乙二醇醚完全溶解后加入苯甲酸钠、其余为去离子水补齐,搅拌均匀后分装保存即可。(2) During the cooling process of step (1), add EDTA and stir to dissolve. When cooled to 40°C, add chlorhexidine acetate and stir to dissolve. After chlorhexidine acetate is completely dissolved, add fatty polyethylene glycol ether (VD-92) Stir to dissolve, add sodium benzoate after the fatty polyethylene glycol ether is completely dissolved, and make up the rest with deionized water, stir evenly, and then pack and store.
实施例3
具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波Example 3
Shampoo with the function of treating pet ectoparasite skin diseases and antibacterial function
本实施例提供100 g具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波,由如下重量份的组分组成:The present embodiment provides 100 g of shampoo with the function of treating pet ectoparasite skin disease and antibacterial function, which is made up of the following components by weight:
非泼罗尼0.2 g、硝酸益康唑2 g、葡萄糖酸氯己定2 g、烷基糖苷(APG)10 g、椰油酰胺丙基甜菜碱(CAB)30 g、十二烷基二甲基氧化胺10g、甲基葡糖二油酸酯(DOE-120)1.5 g、EDTA-2Na 0.1 g、西曲氯铵0.5 g、卡松 0.01g、其余去离子水补齐。Fipronil 0.2 g, Econazole Nitrate 2 g, Chlorhexidine Gluconate 2 g, Alkyl Glycosides (APG) 10 g, Cocamidopropyl Betaine (CAB) 30 g, Dodecyl Dimethyl amine oxide 10g, methylglucodioleate (DOE-120) 1.5g, EDTA-2Na 0.1g, cetrimonium chloride 0.5g, cassone 0.01g, and the rest of the deionized water.
上述具有抗菌功能的动物香波的制备方法为:The preparation method of the above-mentioned animal shampoo with antibacterial function is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到80℃,然后将烷基糖苷、椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入非泼罗尼,待非泼罗尼完全溶解后加入硝酸益康唑,保持在80℃加热下搅拌溶解,待硝酸益康唑充分溶解后加入西曲氯铵,待西曲氯铵完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, heat it to 80 °C, and then add the alkyl glycoside, cocamidopropyl betaine and dodecyl dimethyl amine oxide to the beaker. Stir and dissolve in deionized water, add fipronil after complete dissolution, add econazole nitrate after fipronil is completely dissolved, keep stirring and dissolve under heating at 80 °C, and add cetroxil after econazole nitrate is fully dissolved Ammonium chloride, stop heating after cetrimonium chloride is completely dissolved;
(2)在步骤(1)冷却过程中,加入EDTA-2Na搅拌溶解,冷却至40℃时加入葡萄糖酸氯己定搅拌溶解,待葡萄糖酸氯己定完全溶解后加入甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入卡松、其余为去离子水补齐,搅拌均匀后分装保存即可。(2) During the cooling process of step (1), add EDTA-2Na and stir to dissolve. When cooled to 40°C, add chlorhexidine gluconate and stir to dissolve. After chlorhexidine gluconate is completely dissolved, add methylglucaric dioleic acid. The ester is stirred to dissolve, and after the methyl glucose dioleate is completely dissolved, add Cassone, and the rest is made up with deionized water. After stirring evenly, it can be stored in separate packages.
实施例4
具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波Example 4
Shampoo with the function of treating pet ectoparasite skin diseases and antibacterial function
本实施例提供100 g具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波,由如下重量份的组分组成:The present embodiment provides 100 g of shampoo with the function of treating pet ectoparasite skin disease and antibacterial function, which is made up of the following components by weight:
非泼罗尼0.5g、克霉唑2
g、三氯生1 g、烷基糖苷(APG)5 g、椰油酰胺丙基甜菜碱(CAB)20 g、十二烷基二甲基氧化胺10g、增稠剂-SCC68 1 g、EDTA 0.1 g、氨基硅油0.1 g、脱氢乙酸钠0.01 g、其余为去离子水。Fipronil 0.5g, Clotrimazole 2
g, triclosan 1 g, alkyl glycoside (APG) 5 g, cocamidopropyl betaine (CAB) 20 g, dodecyl dimethyl amine oxide 10 g, thickener-SCC68 1 g, EDTA 0.1 g, amino silicone oil 0.1 g, sodium dehydroacetate 0.01 g, and the rest are deionized water.
上述具有抗菌功能的动物香波的制备方法为:The preparation method of the above-mentioned animal shampoo with antibacterial function is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到70℃,然后将烷基糖苷、椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入非泼罗尼,待非泼罗尼完全溶解后加入克霉唑,保持在70℃加热下搅拌溶解,待克霉唑充分溶解后加入氨基硅油,待氨基硅油完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, heat it to 70 °C, and then add alkyl glycosides, cocamidopropyl betaine and dodecyl dimethyl amine oxide to the beaker. Stir and dissolve in deionized water, add fipronil after complete dissolution, add clotrimazole after fipronil is completely dissolved, keep stirring and dissolve under heating at 70 °C, add amino silicone oil after clotrimazole is fully dissolved, and wait for Stop heating after the amino silicone oil is completely dissolved;
(2)在步骤(1)冷却过程中,加入EDTA搅拌溶解,冷却至50℃时加入三氯生搅拌溶解,待三氯生完全溶解后加入增稠剂-SCC68搅拌溶解,待增稠剂-SCC68完全溶解后加入脱氢乙酸钠、其余为去离子水补齐,搅拌均匀后分装保存即可。(2) During the cooling process of step (1), add EDTA and stir to dissolve. When cooled to 50 °C, add triclosan and stir to dissolve. After the triclosan is completely dissolved, add thickener-SCC68 to stir and dissolve. After the thickener- After the SCC68 is completely dissolved, add sodium dehydroacetate and make up the rest with deionized water, stir evenly, and then pack and store.
实施例5
具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波Example 5
Shampoo with the function of treating pet ectoparasite skin diseases and antibacterial function
本实施例提供100 g具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波,由如下重量份的组分组成:The present embodiment provides 100 g of shampoo with the function of treating pet ectoparasite skin disease and antibacterial function, which is made up of the following components by weight:
非泼罗尼0.1 g、氟康唑1 g、聚六亚甲基双胍1
g、烷基糖苷(APG)5 g、椰油酰胺丙基甜菜碱(CAB)20 g、十二烷基二甲基氧化胺8g、丙烯酸酯共聚物乳液1 g、EDTA-2Na 0.1 g、西曲氯胺1 g、卡松0.01 g、其余为去离子水。Fipronil 0.1 g, Fluconazole 1 g, Polyhexamethylene biguanide 1
g, alkyl glycoside (APG) 5 g, cocamidopropyl betaine (CAB) 20 g, dodecyl dimethyl amine oxide 8 g, acrylate copolymer emulsion 1 g, EDTA-2Na 0.1 g, Western Trichloramine 1 g, Kasone 0.01 g, and the rest are deionized water.
上述具有抗菌功能的动物香波的制备方法为:The preparation method of the above-mentioned animal shampoo with antibacterial function is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到80℃,然后将烷基糖苷、椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入非泼罗尼,待非泼罗尼完全溶解后加入氟康唑,保持在80℃加热下搅拌溶解,待氟康唑充分溶解后加入西曲氯铵,待西曲氯铵完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, heat it to 80 °C, and then add the alkyl glycoside, cocamidopropyl betaine and dodecyl dimethyl amine oxide to the beaker. Stir and dissolve in deionized water, add fipronil after complete dissolution, add fluconazole after fipronil is completely dissolved, keep stirring and dissolve under heating at 80 °C, and add cetrimonium chloride after fluconazole is fully dissolved , stop heating after cetrimonium chloride is completely dissolved;
(2)在步骤(1)冷却过程中,加入EDTA-2Na搅拌溶解,冷却至50℃时加入聚六亚甲基双胍搅拌溶解,待聚六亚甲基双胍完全溶解后加入丙烯酸酯共聚物乳液搅拌溶解,待丙烯酸酯共聚物乳液完全溶解后加入卡松、其余为去离子水补齐,搅拌均匀后分装保存即可。(2) During the cooling process of step (1), add EDTA-2Na and stir to dissolve. When cooled to 50 °C, add polyhexamethylene biguanide to stir and dissolve. After the polyhexamethylene biguanide is completely dissolved, add acrylate copolymer emulsion. Stir to dissolve, add Cassone after the acrylate copolymer emulsion is completely dissolved, and make up the rest with deionized water. After stirring evenly, it can be stored in separate packages.
实施例6
非泼罗尼溶剂筛选Example 6
Fipronil Solvent Screening
由于驱虫剂非泼罗尼难溶于水,而溶于丙酮等对宠物皮肤有害的有机溶剂,这些有机溶剂不能添加到宠物香波体系中,本发明创新地提供了一种可用于宠物香波而且无毒的复配表面活性剂配方来溶解非泼罗尼,不仅解决了非泼罗尼难溶的问题,同时也解决了香波体系发泡稳泡的问题。Since the repellant fipronil is insoluble in water, it is soluble in organic solvents such as acetone, which are harmful to pet skin, and these organic solvents cannot be added to the pet shampoo system. The non-toxic compound surfactant formula to dissolve fipronil not only solves the problem of insoluble fipronil, but also solves the problem of foaming and stable foaming of the shampoo system.
1、单溶剂溶解非泼罗尼筛选试验1. Single-solvent dissolving fipronil screening test
(1)实验方法(1) Experimental method
称取0.2g的驱虫剂非泼罗尼,在温度控制在80 ℃下分别加入20g表1所示的各处方溶剂中,观察驱虫剂非泼罗尼的溶解程度。Weigh 0.2 g of the insect repellant fipronil, and add 20 g of each formulation solvent shown in Table 1 under the temperature control of 80° C. to observe the degree of dissolution of the insect repellant fipronil.
表1Table 1
(2)溶解结果(2) Dissolution results
单一溶剂溶解非泼罗尼的情况如表2所示,从表2中可以看出一些表面活性剂并不能单独溶解非泼罗尼,非泼罗尼是一种难溶物质,在上述表1中部分特定的溶剂中虽然可以溶解,但单独一种溶剂去溶解效果都不理想,都为微溶于水,不能充分溶解非泼罗尼,无法符合产品质量的需求,因此考虑复配的方法来溶解非泼罗尼。The situation of dissolving Fipronil in a single solvent is shown in Table 2. It can be seen from Table 2 that some surfactants cannot dissolve Fipronil alone. Fipronil is an insoluble substance. Although it can be dissolved in some specific solvents, the dissolution effect of a single solvent is not ideal. They are all slightly soluble in water and cannot fully dissolve fipronil, which cannot meet the requirements of product quality. Therefore, the method of compounding is considered. to dissolve fipronil.
表2Table 2
2、两种溶剂复配溶解非泼罗尼筛选试验2. Screening test of two kinds of solvents to dissolve fipronil
(1)实验方法(1) Experimental method
称取0.2 g的驱虫剂非泼罗尼,在温度控制在80 ℃下分别加入表3所示的各处方溶剂中,观察驱虫剂非泼罗尼的溶解程度。Weigh 0.2 g of the repellant fipronil, and add it to each of the formulation solvents shown in Table 3 at a temperature of 80 °C to observe the degree of dissolution of the repellant fipronil.
表3table 3
(2)溶解结果(2) Dissolution results
两种溶剂复配溶解非泼罗尼的情况如表4所示。Table 4 shows the situation of dissolving fipronil in two solvents.
表4Table 4
从表4的溶解情况可以看出,APG和CAB复配,APG和OB-2复配以及CAB和OB-2复配都能较快地完全溶解驱虫剂非泼罗尼,但是处方3、4、10的溶剂复配并不能完全溶解非泼罗尼。From the dissolution situation in Table 4, it can be seen that the combination of APG and CAB, the combination of APG and OB-2, and the combination of CAB and OB-2 can completely dissolve the insect repellant fipronil quickly, but prescription 3, 4 and 10 of the solvent composition cannot completely dissolve fipronil.
APG、CAB和OB-2均为化妆品中常用的表面活性剂,不是非泼罗尼专用的溶剂,但经试验发现表面活性剂复配体系可以较好的溶解难溶成分非泼罗尼。APG, CAB and OB-2 are surfactants commonly used in cosmetics, and are not special solvents for fipronil. However, it has been found that the surfactant compound system can better dissolve the insoluble component fipronil.
3、三种溶剂复配溶解非泼罗尼筛选试验3. Screening test of three kinds of solvents to dissolve fipronil
(1)实验方法(1) Experimental method
称取0.2 g的驱虫剂非泼罗尼,在温度控制在80 ℃下分别加入表5所示的各处方溶剂中,观察驱虫剂非泼罗尼的溶解程度。Weigh 0.2 g of the repellant fipronil, and add it to each of the formulation solvents shown in Table 5 under the temperature control of 80 °C to observe the degree of dissolution of the repellant fipronil.
表5table 5
(2)溶解结果(2) Dissolution results
三种溶剂复配溶解非泼罗尼的情况如表6所示。Table 6 shows the situation of dissolving fipronil in three solvents.
表6Table 6
从表6的溶解时间可以看出,三种溶剂复配溶解非泼罗尼的时间相较于两种溶剂大大缩短,尽管本发明所用的溶剂不是非泼罗尼的专用溶剂,并且单用和两者复配溶解效果不佳,但本发明将三者在合适的比例下进行复配,能较快地完全溶解难溶物质非泼罗尼驱虫剂。As can be seen from the dissolution time in Table 6, the time for dissolving Fipronil in three solvents is greatly shortened compared to the two solvents, although the solvent used in the present invention is not a special solvent for Fipronil, and the single use and The compounding and dissolving effect of the two is not good, but in the present invention, the three are compounded in an appropriate ratio, and the insoluble substance non-prednisolone insect repellant can be dissolved quickly and completely.
实施例7
香波稳定性试验Example 7
Shampoo Stability Test
一、香波的制备1. Preparation of shampoo
按香波总重量为100 g计算。Calculated on a total weight of 100 g of shampoo.
1、实施例3制备的为宠物香波1。1. The pet shampoo 1 prepared in Example 3 is pet shampoo 1.
2、实施例2制备的为宠物香波2。2. The pet shampoo 2 prepared in Example 2 is pet shampoo 2.
3、宠物香波的制备方法为:3. The preparation method of pet shampoo is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到60~80℃,然后将10 g烷基糖苷、20g椰油酰胺丙基甜菜碱加入去离子水中搅拌溶解,完全溶解后再加入0.2 g非泼罗尼,待非泼罗尼完全溶解后加入1 g酮康唑,保持在60~80℃加热下搅拌溶解,待酮康唑充分溶解后加入0.1g氨基硅油微乳液,待氨基硅油完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, and heat it to 60-80 °C, then add 10 g of alkyl glycosides and 20 g of cocamidopropyl betaine into deionized water and stir to dissolve , add 0.2 g of fipronil after it is completely dissolved, add 1 g of ketoconazole after the fipronil is completely dissolved, keep stirring and dissolve under heating at 60-80 °C, and add 0.1 g of amino acid after the ketoconazole is fully dissolved. Silicone oil microemulsion, stop heating after amino silicone oil is completely dissolved;
(2)在步骤(1)冷却过程中,加入0.1gEDTA-2Na搅拌溶解,冷却至40~50℃时加入1 g苯扎溴铵搅拌溶解,待苯扎溴铵完全溶解后加入1 g甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入0.01g山梨酸钾、其余为去离子水补齐,搅拌均匀后分装保存即可得宠物香波3。(2) During the cooling process of step (1), add 0.1 g of EDTA-2Na and stir to dissolve. When cooled to 40-50 °C, add 1 g of benzalkonium bromide and stir to dissolve. After the benzalkonium bromide is completely dissolved, add 1 g of methyl methyl bromide. Gluconate dioleate is stirred and dissolved, and after methyl glucose dioleate is completely dissolved, 0.01 g of potassium sorbate is added, and the rest is made up with deionized water.
4、宠物香波的制备方法为:4. The preparation method of pet shampoo is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到60~80℃,然后将6 g烷基糖苷、6g十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入0.2 g非泼罗尼,待非泼罗尼完全溶解后加入1 g酮康唑,保持在60~80℃加热下搅拌溶解,待酮康唑充分溶解后加入0.1g氨基硅油微乳液,待氨基硅油完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, and heat it to 60-80 °C, then add 6 g of alkyl glycosides and 6 g of dodecyl dimethyl amine oxide into the deionized water. Stir to dissolve, add 0.2 g of fipronil after complete dissolution, add 1 g of ketoconazole after fipronil is completely dissolved, keep stirring and dissolve under heating at 60-80 °C, and add 0.1 g of ketoconazole after fully dissolved g amino silicone oil microemulsion, stop heating after the amino silicone oil is completely dissolved;
(2)在步骤(1)冷却过程中,加入0.1gEDTA-2Na搅拌溶解,冷却至40~50℃时加入1 g苯扎溴铵搅拌溶解,待苯扎溴铵完全溶解后加入1 g甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入0.01g山梨酸钾、其余为去离子水补齐,搅拌均匀后分装保存即可得宠物香波4。(2) During the cooling process of step (1), add 0.1 g of EDTA-2Na and stir to dissolve. When cooled to 40-50 °C, add 1 g of benzalkonium bromide and stir to dissolve. After the benzalkonium bromide is completely dissolved, add 1 g of methyl methyl bromide. Gluconate dioleate is stirred and dissolved, and after methyl glucose dioleate is completely dissolved, 0.01 g of potassium sorbate is added, and the rest is made up with deionized water.
5、宠物香波的制备方法为:5. The preparation method of pet shampoo is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到60~80℃,然后将20g椰油酰胺丙基甜菜碱、6g十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入0.2 g非泼罗尼,待非泼罗尼完全溶解后加入1 g酮康唑,保持在60~80℃加热下搅拌溶解,待酮康唑充分溶解后加入0.1g氨基硅油微乳液,待氨基硅油完全溶解后停止加热;(1) Calculated according to the total volume of 100 mL, add 35 mL of deionized water to the beaker, and heat it to 60-80 °C, then add 20 g of cocamidopropyl betaine and 6 g of dodecyl dimethyl amine oxide into the beaker. Stir and dissolve in deionized water, add 0.2 g of fipronil after complete dissolution, add 1 g of ketoconazole after fipronil is completely dissolved, keep stirring and dissolve under heating at 60-80 °C, and wait until ketoconazole is fully dissolved Then add 0.1g amino silicone oil microemulsion, stop heating after the amino silicone oil is completely dissolved;
(2)在步骤(1)冷却过程中,加入0.1gEDTA-2Na搅拌溶解,冷却至40~50℃时加入1 g苯扎溴铵搅拌溶解,待苯扎溴铵完全溶解后加入1 g甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入0.01g山梨酸钾、其余为去离子水补齐,搅拌均匀后分装保存即可得宠物香波5。(2) During the cooling process of step (1), add 0.1 g of EDTA-2Na and stir to dissolve. When cooled to 40-50 °C, add 1 g of benzalkonium bromide and stir to dissolve. After the benzalkonium bromide is completely dissolved, add 1 g of methyl methyl bromide. Gluconate dioleate is stirred and dissolved. After methyl glucose dioleate is completely dissolved, 0.01 g of potassium sorbate is added, and the rest is made up with deionized water.
二、香波稳定性评价2. Evaluation of shampoo stability
(1)测试方法(1) Test method
泡沫体积:搅动法,分别称取0.5 g步骤一中制备的5种香波样品于100 mL量筒中,加入20 mL纯水,均匀振动100次,于1 min时读取泡沫体积値;Foam volume: by stirring method, weigh 0.5 g of the 5 shampoo samples prepared in step 1 into a 100 mL graduated cylinder, add 20 mL of pure water, shake uniformly 100 times, and read the foam volume at 1 min;
稳定性实验:将制备好的处方样品常温放置统计析出时间。Stability test: The prepared prescription samples were placed at room temperature to count the precipitation time.
(2)实验结果(2) Experimental results
表7Table 7
从表7的结果可以看出,从稳定性试验结果可以看出三种表活复配的体系对非泼罗尼有很好的助溶效果,而对比香波3~5都为两种表活复配,溶解硝酸咪康唑的效果较差而且析出和泡沫体积也低。由此可以看出三种表活复配对香波体系有益,也可以让香波体系更加稳定。As can be seen from the results in Table 7, it can be seen from the results of the stability test that the three kinds of surface active compounds have a good solubilizing effect on fipronil, while the comparison shampoos 3 to 5 are all two kinds of surface active agents. Compounding, the effect of dissolving miconazole nitrate is poor and the precipitation and foam volume are also low. From this, it can be seen that the three types of rejuvenation are beneficial to the shampoo system, and can also make the shampoo system more stable.
实施例8
增稠剂筛选试验Example 8
Thickener Screening Test
由于目前市场上常见香波体系一般都为阴离子体系,而处方中加入的抗细菌剂中大多都是是阳离子表面活性剂,而且加入的调理剂中也有阳离子的表面活性剂,所以本发明选择的非阴离子表面活性剂作为主要表面活性剂,就造成了香波体系难增稠的问题,本实施例试验挑选了常用的一些增稠剂和一些特殊的增稠剂来对香波进行增稠,通过试验对比筛选出最佳的增稠剂。Since the common shampoo systems on the market are generally anionic systems, and most of the antibacterial agents added in the prescription are cationic surfactants, and the added conditioning agents also contain cationic surfactants, the non- As the main surfactant, anionic surfactants cause the problem that the shampoo system is difficult to thicken. In this example, some commonly used thickeners and some special thickeners were selected to thicken the shampoo, and compared through experiments Screen out the best thickener.
1、香波的制备同实施例3,各香波的区别在于添加的增稠剂不同,具体如表8所示。1. The preparation of shampoo is the same as in Example 3, and the difference of each shampoo is that the added thickener is different, as shown in Table 8.
2、实验结果2. Experimental results
(1)增稠效果:将香波放置消去表面气泡,消泡后在粘度仪上测量其粘度。并观察香波放置消泡后的澄清度。(1) Thickening effect: Place the shampoo to eliminate surface air bubbles, and measure its viscosity on a viscometer after defoaming. And observe the clarity of the shampoo after defoaming.
表8Table 8
从表8的实验结果可以看出,香波2、3、4中通过3种高分子聚合物类增稠剂制备的香波粘度虽然够了,但香波整体并不澄清透明,所以这两款增稠剂不予考虑使用。盐类如氯化钠增稠效果不明显且较容易析出从而影响溶液澄清度及稳定性。而氨基酸增稠剂和其他几款增稠剂都有一定增稠作用但以氨基酸增稠剂粘度最佳,其中氨基酸增稠剂有鲸蜡硬脂基葡糖苷、丙烯酸酯共聚物乳液(SF-1)、增稠剂-SCC68、脂肪聚乙二醇醚(VD-92)、甲基葡糖二油酸酯(DOE-120),氨基酸增稠剂能使难增稠的氨基酸表活体系增稠,但在其他体系增稠效果并不明显,而本发明发现氨基酸增稠剂在本专利发明的特殊非阴离子表活体系中也能起到很好的作用。It can be seen from the experimental results in Table 8 that although the viscosity of the shampoo prepared by three kinds of polymer thickeners in shampoo 2, 3 and 4 is sufficient, the overall shampoo is not clear and transparent, so these two thickening agents Agents are not considered for use. Salts such as sodium chloride have less thickening effect and are more likely to precipitate out, thereby affecting the clarity and stability of the solution. The amino acid thickener and several other thickeners have a certain thickening effect, but the amino acid thickener has the best viscosity, among which the amino acid thickener includes cetearyl glucoside, acrylate copolymer emulsion (SF- 1), thickener-SCC68, fatty polyethylene glycol ether (VD-92), methyl glucose dioleate (DOE-120), amino acid thickener can make the amino acid surface active system that is difficult to thicken thicken. Thickening, but the thickening effect is not obvious in other systems, and the invention found that the amino acid thickener can also play a very good role in the special non-anionic surface active system of the patent invention.
(2)反复冻融稳定性试验(2) Repeated freeze-thaw stability test
将上述表8中制备好的添加不同增稠剂的香波样品1、5-9、11和不加增稠剂的空白对照样品一起先放置于-20℃条件下2d,再取出在40℃加速条件下放置2d。循环三次,每次循环都取样并在高效液相色谱仪上检测各药物含量变化,以此推断处方中药物的稳定性情况。The shampoo samples 1, 5-9, 11 prepared in the above table 8 with different thickeners and the blank control sample without thickener were first placed at -20 °C for 2d, and then taken out and accelerated at 40 °C. Condition placed for 2d. Three cycles were performed, and samples were taken in each cycle and the changes in the content of each drug were detected on a high-performance liquid chromatograph to infer the stability of the drugs in the prescription.
表9
反复冻融试验主成分含量结果Table 9
Principal component content results of repeated freeze-thaw test
从表9的实验结果可以看出,处方5、7和9不仅粘度高而且含量变化幅度偏小。It can be seen from the experimental results in Table 9 that formulations 5, 7 and 9 not only have high viscosity but also have a small variation in content.
表10
反复冻融试验处方粘度结果表(mPa•S)Table 10
Table of formula viscosity results of repeated freeze-thaw test (mPa•S)
从表10的结果可知,氨基酸增稠剂不仅能很好的为难增稠香波体系增稠而且具有增加香波体系稳定性的作用。It can be seen from the results in Table 10 that the amino acid thickener can not only thicken the refractory thickening shampoo system well but also have the effect of increasing the stability of the shampoo system.
实施例9 制备工艺筛选和优化Example 9 Preparation process screening and optimization
1、非泼罗尼溶解最佳温度筛选试验1. Screening test of optimal temperature for dissolving fipronil
本实施例提供100 g具有治疗宠物体外寄生虫皮肤病功能和抗菌功能的香波,由如下重量份的组分组成:The present embodiment provides 100 g of shampoo with the function of treating pet ectoparasite skin disease and antibacterial function, which is made up of the following components by weight:
非泼罗尼0.2 g、硝酸益康唑2 g、葡萄糖酸氯己定2 g、烷基糖苷(APG)10 g、椰油酰胺丙基甜菜碱(CAB)30 g、十二烷基二甲基氧化胺10g、甲基葡糖二油酸酯(DOE-120)1.5 g、EDTA-2Na 0.1 g、西曲氯铵0.5 g、卡松 0.01g、其余去离子水补齐。Fipronil 0.2 g, Econazole Nitrate 2 g, Chlorhexidine Gluconate 2 g, Alkyl Glycosides (APG) 10 g, Cocamidopropyl Betaine (CAB) 30 g, Dodecyl Dimethyl amine oxide 10g, methylglucodioleate (DOE-120) 1.5g, EDTA-2Na 0.1g, cetrimonium chloride 0.5g, cassone 0.01g, and the rest of the deionized water.
上述具有抗菌功能的动物香波的制备方法为:The preparation method of the above-mentioned animal shampoo with antibacterial function is:
(1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,按照表11中各工艺的温度分别加热到30℃、40℃、50℃、60℃、70℃、80℃、90℃,然后将烷基糖苷、椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺加入去离子水中搅拌溶解,完全溶解后再加入非泼罗尼,待非泼罗尼完全溶解后加入硝酸益康唑,保持在各温度加热下搅拌溶解,待硝酸益康唑充分溶解后加入西曲氯铵,待西曲氯铵完全溶解后停止加热;(1) Calculated based on the total volume of 100 mL, add 35 mL of deionized water to the beaker, and heat it to 30 °C, 40 °C, 50 °C, 60 °C, 70 °C, 80 °C, 90 °C according to the temperature of each process in Table 11. ℃, then add alkyl glycoside, cocamidopropyl betaine and dodecyl dimethyl amine oxide into deionized water and stir to dissolve, and then add fipronil after complete dissolution, and after fipronil is completely dissolved Add econazole nitrate, keep stirring and dissolve under heating at various temperatures, add cetrimonium chloride after econazole nitrate is fully dissolved, and stop heating after cetrimonium chloride is completely dissolved;
(2)在步骤(1)冷却过程中,加入EDTA-2Na搅拌溶解,冷却至40℃时加入葡萄糖酸氯己定搅拌溶解,待葡萄糖酸氯己定完全溶解后加入甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入卡松、其余为去离子水补齐,搅拌均匀后分装保存即可。(2) During the cooling process of step (1), add EDTA-2Na and stir to dissolve. When cooled to 40°C, add chlorhexidine gluconate and stir to dissolve. After chlorhexidine gluconate is completely dissolved, add methylglucaric dioleic acid. The ester is stirred to dissolve, and after the methyl glucose dioleate is completely dissolved, add Cassone, and the rest is made up with deionized water. After stirring evenly, it can be stored in separate packages.
表11 非泼罗尼溶解最佳温度筛选试验结果表Table 11 Results of the screening test for the optimal temperature for dissolving fipronil
“-”为不溶解"-" means insoluble
从温度筛选试验结果可知,在70~80℃条件下溶解时间较短,其中80℃最优,90℃粘度虽然与70℃差不多但粘度相比80℃有下降,温度越高成本越高。所以最佳温度为80℃。From the results of the temperature screening test, it can be seen that the dissolution time is shorter under the condition of 70~80℃, of which 80℃ is the best. Although the viscosity at 90℃ is similar to that of 70℃, the viscosity is lower than that at 80℃. The higher the temperature, the higher the cost. So the optimum temperature is 80°C.
2、加料顺序优化2. Optimization of feeding sequence
香波的配方和参数条件同上述工艺6。The formula and parameter conditions of the shampoo are the same as the above-mentioned process 6.
(1)工艺8:(1) Process 8:
(1-1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到80℃,先加入处方量的非泼罗尼,然后加入处方量的烷基糖苷、加热搅拌十分钟加入椰油酰胺丙基甜菜碱和十二烷基二甲基氧化胺搅拌溶解,完全溶解后再加入硝酸益康唑,保持在80℃加热下搅拌溶解,待硝酸益康唑充分溶解后加入西曲氯铵,待西曲氯铵完全溶解后停止加热;(1-1) Calculated according to the total volume of 100mL, add 35mL of deionized water to the beaker, heat it to 80°C, first add the prescribed amount of fipronil, then add the prescribed amount of alkyl glycosides, heat and stir for ten times. Add cocamidopropyl betaine and dodecyl dimethyl amine oxide and stir to dissolve, add econazole nitrate after complete dissolution, keep stirring and dissolve under heating at 80 °C, and add econazole nitrate after fully dissolved Cetrimonium chloride, stop heating after cetrimonium chloride is completely dissolved;
(1-2)在步骤(1)冷却过程中,加入EDTA-2Na搅拌溶解,冷却至40℃时加入葡萄糖酸氯己定搅拌溶解,待葡萄糖酸氯己定完全溶解后加入甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入卡松、其余为去离子水补齐,搅拌均匀后分装保存即可。(1-2) During the cooling process of step (1), add EDTA-2Na and stir to dissolve. When cooled to 40 °C, add chlorhexidine gluconate and stir to dissolve. After the chlorhexidine gluconate is completely dissolved, add methylglucose two The oleic acid ester is stirred and dissolved. After the methyl glucose dioleate is completely dissolved, add Cassone, and the rest is made up with deionized water. After stirring evenly, it can be stored in separate packages.
(2)工艺9
:
(2) Process 9 :
(1-1)按总体积为100mL计算,在烧杯中加入35mL的去离子水,并加热到80℃,然后先加入烷基糖苷加热搅拌、完全溶解后加入非泼罗尼,加热搅拌10分钟后加入椰油酰胺丙基甜菜碱和西曲氯胺,加热搅拌十分钟后加入十二烷基二甲基氧化胺,完全溶解后再加入硝酸益康唑,保持在80℃加热下搅拌溶解,待硝酸益康唑充分溶解后停止加热;(1-1) Calculated according to the total volume of 100 mL, add 35 mL of deionized water to the beaker, and heat it to 80 °C, then add the alkyl glycoside, heat and stir, completely dissolve, then add fipronil, and heat and stir for 10 minutes Then add cocamidopropyl betaine and cetrichloramine, add dodecyl dimethyl amine oxide after heating and stirring for ten minutes, add econazole nitrate after complete dissolution, keep stirring and dissolving under heating at 80 ℃, Stop heating after econazole nitrate is fully dissolved;
(1-2)在步骤(1)冷却过程中,加入EDTA-2Na搅拌溶解,冷却至40℃时加入葡萄糖酸氯己定搅拌溶解,待葡萄糖酸氯己定完全溶解后加入甲基葡糖二油酸酯搅拌溶解,待甲基葡糖二油酸酯完全溶解后加入卡松、其余为去离子水补齐,搅拌均匀后分装保存即可。(1-2) During the cooling process of step (1), add EDTA-2Na and stir to dissolve. When cooled to 40 °C, add chlorhexidine gluconate and stir to dissolve. After the chlorhexidine gluconate is completely dissolved, add methylglucose two The oleic acid ester is stirred and dissolved. After the methyl glucose dioleate is completely dissolved, add Cassone, and the rest is made up with deionized water. After stirring evenly, it can be stored in separate packages.
(3)观察非泼罗尼从加入至完全溶解的时间以及香波整体粘度,统计结果如表12所示。(3) Observe the time from the addition of fipronil to complete dissolution and the overall viscosity of the shampoo. The statistical results are shown in Table 12.
表12 不同工艺试验结果表Table 12 Results of different process tests
实施例10
宠物犬猫临床寄生虫性皮肤病治疗效果试验Example 10
Clinical trial of the therapeutic effect of pet dogs and cats on parasitic skin diseases
1、试验动物1. Experimental animals
选取广东省云浮市新兴雷米高宠物犬场犬猫寄生虫性皮肤病自然发病病例共计40头。其中犬20头,猫20头。A total of 40 natural cases of parasitic skin diseases of dogs and cats in Xinxing Remigao pet dog farm in Yunfu City, Guangdong Province were selected. There are 20 dogs and 20 cats.
2、实验方法2. Experimental method
(1)临床检查:进行临床全身检查,包括体温、呼吸、心率等常规指标;局部检查体表瘙痒程度、皮肤破损程度、红疹或脓疹的数量,皮肤病灶的部位与分布情况。(1) Clinical examination: carry out clinical general examination, including routine indicators such as body temperature, respiration, heart rate, etc.; local examination of the degree of pruritus on the body surface, the degree of skin damage, the number of rashes or pustules, and the location and distribution of skin lesions.
(2)体外寄生虫的检查:取患部皮肤,显微镜下观察并计算局部病灶中外寄生虫的数量。对于跳蚤感染:计算与统计跳蚤数量、蚤粪分布与数量;对于蜱叮咬性皮炎:计算与统计蜱的数量和皮肤病损情况。(2) Inspection of ectoparasites: Take the skin of the affected part, observe under a microscope and count the number of ectoparasites in the local lesions. For flea infestation: count and count the number of fleas, distribution and quantity of flea feces; for tick bite dermatitis: count and count the number of ticks and skin lesions.
(3)给药方法(3) Administration method
用水浸湿犬猫的毛发,将香波涂在全身,避免眼睛、鼻子和嘴巴。洗发并按摩,确保产品与皮肤及毛皮接触10分钟。用水冲洗干净,可带上伊丽莎白圈确保犬猫在洗澡时不舔自己或是泡沫。试验药物组每周使用两次实施例3的香波,香波每次使用量为1-2mg/kg动物;空白组为不使用香波作为对照。Wet the dog or cat's hair with water and apply the shampoo all over the body, avoiding the eyes, nose and mouth. Shampoo and massage, making sure the product is in contact with the skin and fur for 10 minutes. Rinse off with water and wear an Elizabethan ring to ensure dogs and cats don't lick themselves or lather while bathing. The experimental drug group used the shampoo of Example 3 twice a week, and the amount of the shampoo used each time was 1-2 mg/kg animal; the blank group was not using the shampoo as a control.
(4)疗效评价指标:(4) Efficacy evaluation indicators:
有效:用药治疗期间和用药结束后,皮肤的体外寄生虫数量平均减少率≥
90%,患部无瘙痒,无红疹、皮屑或试验动物自我啃咬的损伤。Effective: The average reduction rate of the number of ectoparasites in the skin during and after the drug treatment period is ≥
In 90% of cases, there was no itching in the affected area, no rash, dandruff or self-biting injury of the experimental animals.
无效:用药治疗期间和用药结束后,皮肤的体外寄生虫数量平均减少率<90%,患部仍有瘙痒,红疹、皮屑等,动物自我啃咬性损伤明显存在。Ineffective: During and after the drug treatment, the average reduction rate of ectoparasites on the skin is less than 90%, the affected part is still itchy, rash, dander, etc., and the animal self-gnawing injury is obvious.
复发率监测:对治愈后的犬猫进行停药后1周内的皮肤临床症状及皮肤寄生虫数量监测。Monitoring of recurrence rate: The clinical symptoms of the skin and the number of skin parasites were monitored within 1 week after drug withdrawal in the cured dogs and cats.
3、实验结果3. Experimental results
(1)疗效评价结果(1) Efficacy evaluation results
表13
宠物犬猫临床寄生虫性皮肤病治疗效果试验结果Table 13
The results of the clinical trial of clinical parasitic skin diseases in pet dogs and cats
从表13可以看出,本发明的宠物香波对患有寄生虫性皮肤病犬的治愈率达到100%,对患有寄生虫性皮肤病猫的治愈率达到90%。As can be seen from Table 13, the cure rate of the pet shampoo of the present invention to dogs suffering from parasitic skin disease reaches 100%, and the cure rate to cats suffering from parasitic skin disease reaches 90%.
(2)复发率监测结果(2) Monitoring results of recurrence rate
停药后1周内观察,试验治疗组中,10只犬患寄生虫性皮肤病治愈后未见有复发病例,9只猫寄生虫性皮肤病治愈后有1例复发寄生虫性皮肤病;可见,本发明的香波施用后,可以有效的驱除宠物体表的寄生虫,让宠物免于受寄生虫的困扰。Observed within 1 week after drug withdrawal, in the experimental treatment group, 10 dogs were cured of parasitic skin disease and there was no recurrence case, and 9 cats were cured of parasitic skin disease and had 1 case of recurrent parasitic skin disease; It can be seen that after the shampoo of the present invention is applied, the parasites on the body surface of the pet can be effectively expelled, so that the pet is free from the trouble of parasites.
实施例10
宠物犬猫临床真菌性皮肤病治疗效果试验Example 10
Trial of the therapeutic effect of clinical fungal skin disease in pet dogs and cats
1、试验动物1. Experimental animals
选取广东省云浮市新兴雷米高宠物犬场犬场犬猫真菌性皮肤病自然发病病例共计40只。其中犬20只,猫20只。A total of 40 cases of natural onset of dog and cat fungal skin disease in Xinxing Remigao Pet Dog Farm, Yunfu City, Guangdong Province were selected. Among them, there are 20 dogs and 20 cats.
2、实验方法2. Experimental method
(1)临床检查:进行临床全身和局部检查,以及必要的皮肤刮片检查(1) Clinical examination: perform clinical general and local examinations, as well as necessary skin scraping examinations
(2)微生物学检查:对病例动物从患部采集样品,进行致病菌分离并鉴定,最后进行致病菌计数。(2) Microbiological examination: collect samples from the affected part of the case animals, isolate and identify the pathogenic bacteria, and finally count the pathogenic bacteria.
(3)给药方法(3) Administration method
用水浸湿犬猫的毛发,将香波涂在全身,避免眼睛、鼻子和嘴巴。洗发并按摩,确保产品与皮肤及毛皮接触10分钟。用水冲洗干净,可带上伊丽莎白圈确保犬猫在洗澡时不舔自己或是泡沫。试验药物组(实施例3的香波)每周使用两次,香波每次使用量为1-2mg/kg动物。空白组为不使用香波作为对照。Wet the dog or cat's hair with water and apply the shampoo all over the body, avoiding the eyes, nose and mouth. Shampoo and massage, making sure the product is in contact with the skin and fur for 10 minutes. Rinse off with water and wear an Elizabethan ring to ensure dogs and cats don't lick themselves or lather while bathing. The experimental drug group (the shampoo of Example 3) was used twice a week, and the amount of the shampoo used each time was 1-2 mg/kg of animals. The blank group used no shampoo as a control.
(4)疗效评价指标(4) Efficacy evaluation index
治愈:用药后,皮肤的致病真菌的分离转阴率≥80%,同时炎性分泌物显著消失,患部无异味,红疹、皮屑、脓疹等显著消失,瘙痒症状显著消失。Cure: After taking the medicine, the isolation rate of pathogenic fungi on the skin is ≥80%, and the inflammatory secretions disappear significantly, the affected part has no peculiar smell, the rashes, dandruff, pustules, etc. disappear significantly, and the itching symptoms disappear significantly.
有效:用药后,皮肤的致病真菌的分离转阴率≥60%,同时炎性分泌物减少,患部基本无异味,红疹、皮屑、脓疹等减少,瘙痒症状减轻。Effective: After the medication, the isolation rate of pathogenic fungi on the skin is ≥60%, and the inflammatory secretions are reduced, the affected area is basically free of peculiar smell, the rash, dandruff, pustules, etc. are reduced, and the itching symptoms are relieved.
无效:用药后,皮肤的致病真菌的分离转阴率<60%,同时炎性分泌物依然存在,患部仍有异味,红疹、皮屑、脓疹等未见减少,患部仍有明显的瘙痒症状。Ineffective: After the medication, the isolation rate of pathogenic fungi on the skin is less than 60%, and the inflammatory secretions still exist, the affected part still has peculiar smell, the rash, dandruff, pustules, etc. have not been reduced, and the affected part still has obvious symptoms. Itching symptoms.
(5)复发率监测:对治愈后的犬猫进行停药后1
周内的皮肤临床症状及皮肤微生物学检查。(5) Monitoring of recurrence rate: After stopping the treatment for cured dogs and cats 1
Skin clinical symptoms and skin microbiological examination within a week.
3、试验结果3. Test results
(1)疗效评价结果(1) Efficacy evaluation results
表14
宠物犬猫临床真菌性皮肤病治疗效果试验结果Table 14
Experimental results of clinical fungal skin diseases in pet dogs and cats
从表14可以看出,本发明的宠物香波对患有真菌性皮肤病的犬和猫的治愈率高达90%。As can be seen from Table 14, the cure rate of the pet shampoo of the present invention to dogs and cats with fungal skin diseases is as high as 90%.
(2)复发率监测结果(2) Monitoring results of recurrence rate
停药后1周内观察,试验治疗组中,9只犬患真菌性皮肤病治愈后未见复发病例,9只猫真菌性皮肤病病治愈后有1例复发真菌性皮肤病;可见,本发明的香波施用后,可以有效的改善宠物真菌性皮肤病,且复发率较低。Observation within 1 week after drug withdrawal showed that in the experimental treatment group, 9 dogs with fungal skin disease had no recurrence after being cured, and 1 case of recurrent fungal skin disease was found in 9 cats after being cured; After the shampoo of the invention is applied, the pet fungal skin disease can be effectively improved, and the recurrence rate is low.
实施例11
宠物犬猫临床细菌性皮肤病治疗效果试验Example 11
Experiment on the therapeutic effect of clinical bacterial skin disease in pet dogs and cats
1、试验动物1. Experimental animals
选取广东省云浮市新兴雷米高宠物犬场犬场犬猫细菌性皮肤病自然发病病例共计40只。其中犬20只,猫20只。A total of 40 cases of naturally occurring bacterial skin diseases of dogs and cats in Xinxing Remigao Pet Dog Farm, Yunfu City, Guangdong Province were selected. Among them, there are 20 dogs and 20 cats.
2、实验方法2. Experimental method
(1)临床检查:进行临床全身和局部检查,以及必要的皮肤刮片检查(1) Clinical examination: perform clinical general and local examinations, as well as necessary skin scraping examinations
(2)微生物学检查:对病例动物从患部采集样品,进行致病菌分离并鉴定,最后进行致病菌计数。(2) Microbiological examination: collect samples from the affected part of the case animals, isolate and identify the pathogenic bacteria, and finally count the pathogenic bacteria.
(3)给药方法(3) Administration method
用水浸湿犬猫的毛发,将香波涂在全身,避免眼睛、鼻子和嘴巴。洗发并按摩,确保产品与皮肤及毛皮接触10分钟。用水冲洗干净,可带上伊丽莎白圈确保犬猫在洗澡时不舔自己或是泡沫。试验药物组(实施例3的香波)每周使用两次,香波每次使用量为1-2mg/kg动物。空白组为不使用香波作为对照。。Wet the dog or cat's hair with water and apply the shampoo all over the body, avoiding the eyes, nose and mouth. Shampoo and massage, making sure the product is in contact with the skin and fur for 10 minutes. Rinse off with water and wear an Elizabethan ring to ensure dogs and cats don't lick themselves or lather while bathing. The experimental drug group (the shampoo of Example 3) was used twice a week, and the amount of the shampoo used each time was 1-2 mg/kg of animals. The blank group used no shampoo as a control. .
(4)疗效评价指标(4) Efficacy evaluation index
治愈:用药后,皮肤的致病细菌的分离转阴率≥80%,同时炎性分泌物显著消失,患部无异味,红疹、皮屑、脓疹等显著消失,瘙痒症状显著消失。Cure: After taking the medicine, the isolation and conversion rate of pathogenic bacteria on the skin is ≥80%, and the inflammatory secretions disappear significantly, the affected part has no peculiar smell, the rash, dandruff, pustules, etc. disappear significantly, and the itching symptoms disappear significantly.
有效:用药后,皮肤的致病细菌的分离转阴率≥60%,同时炎性分泌物减少,患部基本无异味,红疹、皮屑、脓疹等减少,瘙痒症状减轻。Effective: After taking the medicine, the isolation rate of pathogenic bacteria on the skin is ≥60%, and the inflammatory secretions are reduced, the affected area is basically free of peculiar smell, the rash, dandruff, pustules, etc. are reduced, and the itching symptoms are relieved.
无效:用药后,皮肤的致病细菌的分离转阴率<60%,同时炎性分泌物依然存在,患部仍有异味,红疹、皮屑、脓疹等未见减少,患部仍有明显的瘙痒症状。Ineffective: After the medication, the isolation rate of pathogenic bacteria on the skin is less than 60%, and the inflammatory secretions still exist, the affected area still has peculiar smell, the rash, dandruff, pustules, etc. have not been reduced, and the affected area still has obvious symptoms. Itching symptoms.
(5)复发率监测:对治愈后的犬猫进行停药后1周内的皮肤临床症状及皮肤微生物学检查。(5) Monitoring of recurrence rate: The clinical symptoms and skin microbiological examinations of the cured dogs and cats were conducted within 1 week after drug withdrawal.
3、试验结果3. Test results
(1)疗效评价结果(1) Efficacy evaluation results
表15
宠物犬猫临床细菌性皮肤病治疗效果试验结果Table 15
Experimental results of clinical treatment of bacterial skin diseases in pet dogs and cats
从表15可以看出,本发明的宠物香波对患有细菌性皮肤病的犬的治愈率达90%,对患有细菌性皮肤病猫的治愈率达100%。As can be seen from Table 15, the pet shampoo of the present invention has a cure rate of 90% for dogs with bacterial skin disease, and a cure rate of 100% for cats with bacterial skin disease.
(2)复发率监测结果(2) Monitoring results of recurrence rate
停药后1周内观察,试验治疗组中,9只犬患细菌性皮肤病治愈后未见有复发病例,10只猫细菌性皮肤病病治愈后未见有复发细菌性皮肤病,可见,本发明的香波施用后,可以有效的改善宠物细菌性皮肤病,且复发率较低。Observed within 1 week after drug withdrawal, in the experimental treatment group, 9 dogs with bacterial skin disease had no recurrence after being cured, and 10 cats had no recurrence of bacterial skin disease after being cured. It can be seen that, After the shampoo of the present invention is applied, the pet bacterial skin disease can be effectively improved, and the recurrence rate is low.
最后应当说明的是,以上实施例仅用以说明本发明的技术方案而非对本发明保护范围的限制,对于本领域的普通技术人员来说,在上述说明及思路的基础上还可以做出其它不同形式的变化或变动,这里无需也无法对所有的实施方式予以穷举。凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等,均应包含在本发明权利要求的保护范围之内。Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit the scope of protection of the present invention. For those of ordinary skill in the art, on the basis of the above descriptions and ideas, other Variations or variations in different forms need not and cannot be exhaustive of all implementations here. Any modifications, equivalent replacements and improvements made within the spirit and principle of the present invention shall be included within the protection scope of the claims of the present invention.