WO2022075992A1 - Dispositifs à auto-capillarité - Google Patents

Dispositifs à auto-capillarité Download PDF

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Publication number
WO2022075992A1
WO2022075992A1 PCT/US2020/054917 US2020054917W WO2022075992A1 WO 2022075992 A1 WO2022075992 A1 WO 2022075992A1 US 2020054917 W US2020054917 W US 2020054917W WO 2022075992 A1 WO2022075992 A1 WO 2022075992A1
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WO
WIPO (PCT)
Prior art keywords
region
analyte
charge
fluid
concentrating
Prior art date
Application number
PCT/US2020/054917
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English (en)
Inventor
Tynan A. BECKER
Adam C. WEISMAN
Anita Rogacs
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Hewlett-Packard Development Company, L.P.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hewlett-Packard Development Company, L.P. filed Critical Hewlett-Packard Development Company, L.P.
Priority to PCT/US2020/054917 priority Critical patent/WO2022075992A1/fr
Publication of WO2022075992A1 publication Critical patent/WO2022075992A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Definitions

  • Self-wicking devices are intended to detect the presence of a target analyte in a sample fluid. These devices are simple and can be used typically without specialized training by the user. Accordingly, self-wicking devices can be widely used for medical diagnostic testing, environmental sample testing, and in laboratories, to name a few.
  • FIG.1 graphically illustrates an example self-wicking device in accordance with the present disclosure
  • FIG.2 graphically illustrates an example self-wicking system in accordance with the present disclosure
  • FIG.3 graphically illustrates a method of concentrating and detecting an analyte in a sample fluid in accordance with the present disclosure.
  • DETAILED DESCRIPTION can permit the detection of a target analyte in a sample fluid. These devices can incorporate a porous substrate. The sample fluid sequentially runs along the porous substrate via capillary flow.
  • the target analyte When a target analyte is present in the sample fluid, the target analyte will interact with a compound having a functional group that is capable of binding to the target analyte.
  • a testing region on the porous substrate can detect a complex of the target analyte and the compound and a control region on the porous substrate can detect the compound.
  • an optical indicator When a complex of the target analyte and the compound is detected in the testing region, an optical indicator will appear or will not appear, depending on the device type.
  • an optical indicator When the compound is detected in the control region, an optical indicator will appear, such as a line.
  • Self-wicking devices may be limited in use due to detection limits of these devices.
  • a self-wicking device can include a fluid flow channel including a porous substrate for sequential fluid flow through multiple regions.
  • the multiple regions of the porous substrate can include a complexing region including a compound that can have a functional group to bind with an analyte in a sample fluid to form an analyte complex; a charge adjusting region to provide charge modulation by modifying a charge of the analyte complex or providing a modified concentration of charged centers to interact with the analyte complex; a concentrating region that can include an immobilized material having an affinity to the analyte complex after the charge modulation; and a detecting region that can receive concentrated analyte from the concentrating region.
  • modification of the charge of the analyte complex in the charge adjusting region can occur by a change in pH therein of from about 1 pH unit to about 3.5 pH units relative to a pH of fluid of the complexing region.
  • a pH of the fluid in the complexing region can be about 6.5 to about 7.5
  • a pH of the fluid in the charge adjusting region can be about 4 to about 6 or from about 8 to about 10.
  • the charge adjusting region, the concentrating region, or a combination thereof includes charged centers including a divalent cation.
  • the divalent cation can be selected from a calcium ion, a magnesium ion, a barium ion, a strontium ion, or a combination thereof.
  • the charge adjusting region and the concentrating region are at a common location onof the porous substrate.
  • the device can further include a fluid input port to flow a sample fluid into the fluid flow channel and the fluid input port can be available to subsequently flow an elution fluid therethrough, or the device can further include a separate elution fluid input port positioned to flow an elution fluid at or upstream of the concentrating region.
  • a self-wicking system can include a self-wicking device and a sample fluid containing the analyte to flow through the fluid flow channel.
  • the self-wicking device can include a fluid flow channel including a porous substrate for sequential fluid flow through multiple regions.
  • the multiple regions can include a complexing region including a compound that can have a functional group to bind with an analyte in a sample fluid to form an analyte complex; a charge adjusting region to provide charge modulation by modifying a charge the analyte complex or providing charged centers to interact with the analyte complex; a concentrating region that can include an immobilized material having an affinity with the analyte complex after the charge modulation; and a detecting region that can receive concentrated analyte from the concentrating region.
  • modification of the charge of the analyte complex in the charge adjusting region can occur by a change in pH of the sample fluid therein of from about 1 pH unit to about 3.5 pH units relative to a pH of the sample fluid in the complexing region, or wherein the charge adjusting region, the concentrating region, or a combination thereof includes charged centers that can include a divalent cation.
  • the system can further include an elution fluid to decouple the analyte complex from the immobilized material.
  • a pH of fluid in the complexing region can be from about 6.5 to about 7.5, and a pH of fluid in the charge adjusting region can be from about 4 to about 6 and the elution fluid can include a tris base, a dibasic sodium phosphate, magnesium oxide, disodium hydrogen phosphate, arginine, sodium bicarbonate, ammonium bicarbonate, sodium carbonate, borate, glycine-NaOH, or a combination thereof; or can be from about 8 to about 10 and the elution fluid can include citric acid, tris-HCl, monobasic sodium phosphate, tartaric acid, succinic acid, acetic acid, phosphoric acid, glycerine-HCl, sodium acetate, or a combination thereof.
  • the charge adjusting region, the concentrating region, or the combination thereof can include the divalent cation and the elution fluid can include EDTA, EGTA, GLDA, trisodium ⁇ -DL-alanine diacetate, phytic acid, tetrasodium iminodisuccinate, or a combination thereof.
  • a method of concentrating and detecting an analyte in a sample (“method”) is disclosed herein. The method can include flowing a sample fluid along a fluid flow channel including a porous substrate, where relative to fluid flow, the fluid flow channel sequentially includes a complexing region, a charge adjusting region, a concentrating region, and a detecting region.
  • the method can also include binding an analyte carried by the sample fluid to a compound having a function group to bind with the analyte in the complexing region to form an analyte complex, and modulating a charge by modifying a charge of the analyte complex in the charge adjusting region or providing a modification of charged centers in the charge adjusting region or the concentrating region.
  • the method can further include immobilizing the analyte complex in the concentrating region, releasing concentrated analyte from the concentrating region, and detecting the concentrated analyte in the detecting region.
  • modulating the charge occurs by modifying the charge of the analyte complex in the charge adjusting region by changing a pH of the sample fluid therein of from about 1 pH unit to about 3.5 pH units relative to a pH of fluid in the complexing region, or providing a divalent cation loaded in the charge adjusting region.
  • the releasing of the concentrated analyte can include passing an elution fluid through the concentrating region.
  • FIG.1 and FIG.2 depict various self-wicking devices and systems, respectively, and FIG.3 depicts an example method.
  • FIGS.1-2 depict various self-wicking devices and systems, respectively, and FIG.3 depicts an example method.
  • a self-wicking device 100 can include a fluid flow channel including a porous substrate 110 for sequential fluid flow through multiple regions.
  • the multiple regions can include a complexing region 120 that can include a compound 122 having a functional group to bind with an analyte in a sample fluid to form an analyte complex, a charge adjusting region 130 configured to provide charge modulation by modifying a charge of the analyte complex or providing a modified concentration of charged centers to interact with the analyte complex, a concentrating region 140 that can include an immobilized material 142 with an affinity to the analyte complex after the charge modulation, and a detecting region 150 that can receive concentrated analyte from the concentrating region.
  • the charge adjusting region and the concentrating region can overlap or be within the same physical area, but can provide both functions.
  • the detecting region can include a testing strip 152 and a control strip 154 as shown in FIG.1.
  • the fluid flow channel can include a negative space that can be etched, molded, or engraved from a material of a housing and may surround the porous substrate of a self-wicking device.
  • the fluid flow channel can have a channel size that can range from about 5 ⁇ m to about 15 mm in diameter. In yet other examples, the fluid flow channel can have a diameter that can range from about 5 ⁇ m to about 1,000 ⁇ m, from about 1 mm to about 15 mm, from about 100 ⁇ m to about 500 ⁇ m, from about 500 ⁇ m to about 1,000 ⁇ m, or from about 5 mm to about 10 mm, etc.
  • the fluid flow channel may include a pathway.
  • the pathway may be a linear pathway, a curved path, a pathway with turns, a branched pathway, a serpentine pathway, or any other pathway configuration.
  • the pathway may be linear and/or branched.
  • the porous substrate may be present in a portion of or throughout the entire length of the fluid flow channel.
  • the porous substrate may include a fibrous substrate that can allow a sample fluid to flow therethrough via capillary action.
  • the porous substrate can include nitrocellulose, cellulose, acetate cellulose, fiberglass, porous silica, polyester, surface modified polyester, hydrogel, or a combination thereof.
  • the porous substrate can include a nitrocellulose pad, a cellulose pad, or a fiberglass pad. In another example, the porous substrate can include a nitrocellulose pad. Pores of the porous substrate can have an average pore size ranging from about 500 nm to about 10 ⁇ m, from about 1 ⁇ m to about 10 ⁇ m, from about 5 ⁇ m to about 10 ⁇ m, from about 500 nm to about 5 ⁇ m, or from about 2 ⁇ m to about 8 ⁇ m. A thickness of the porous substrate can range from about 0.1 mm to about 1 mm, from about 0.5 mm to about 1 mm, or from about 0.2 mm to about 0.8 mm.
  • a length of the porous substrate can range from about 1 mm to about 10 mm, from about 5 mm to about 10 mm, from about 1 mm to about 5 mm, or from about 2 mm to about 8 mm.
  • a width of the porous substrate can range from about 1 mm to about 20 mm, from about 5 mm to about 15 mm, or from about 8 mm to about 16 mm.
  • the porous substrate can include multiple regions that can be designed to interact with an analyte in a sample fluid.
  • the porous substrate can include a complexing region, a charge adjusting region, a concentrating region, and a detecting region.
  • the complexing region in further detail can be a hydrophilic region and can have a pH ranging from about 6.5 to about 7.5, from about 7 to about 7.5, or from about 6.5 to about 7.
  • the complexing region can be impregnated with a compound having a functional group that can bind with an analyte in a sample fluid to form an analyte complex.
  • the complexing region can release the compound having the functional group to bind with the analyte in the sample upon application and movement of a sample fluid therethrough.
  • the compound having a functional group to bind with an analyte in a sample may vary based on the analyte and the purpose of the self-wicking device.
  • the compound having the functional group to bind with the analyte in the sample fluid can include a colloidal gold, a colored latex particle, a fluorescent latex particle, a paramagnetic latex particle, a cellulose nanobead, a florescent tag, or a combination thereof.
  • the compound having the functional group to bind with the analyte can be a detection moiety that can be detected in a control strip of the detecting region; thereby indicating that a sample fluid has passed through the self-wicking device.
  • the charge adjusting region can provide charge modulation.
  • the charge modulation may include modifying a charge of the analyte.
  • the charge modulation may include providing a modified concentration of charged centers to interact with the analyte complex.
  • adjusting the charge of the analyte complex can occur by adjusting a pH of the sample fluid that the analyte complex is in.
  • Modification of the charge of the analyte complex in the charge adjusting region can occur by a change in pH of the sample fluid therein of from about 1 pH unit to about 3.5 pH units relative to a pH of the sample fluid in the complexing region.
  • a pH of the sample fluid in the complexing region can range from about 6.5 to about 7.5.
  • a pH of the sample fluid can be reduced to a pH ranging from about 4 to about 6, from about 4.5 to about 5.5, or from about 5 to about 6.
  • a pH of the sample fluid can be increased to a pH ranging from about 8 to about 10, from about 9 to about 10, or from about 8.5 to about 9.5.
  • providing charge modulation can occur by providing a modified concentration of charged centers.
  • the charged centers can include a divalent cation.
  • the divalent cation can be selected from a calcium ion, a magnesium ion, a barium ion, a strontium ion, or a combination thereof.
  • the divalent cation can be selected from a calcium ion, a magnesium, or a combination thereof.
  • the divalent cation can be located in the charge adjusting region, the concentrating region, or a combination thereof.
  • the analyte complex may become temporarily trapped in the concentrating region.
  • An immobilized material in the concentrating region can have an affinity with the analyte complex.
  • affinity with indicates that the immobilized material is capable of attracting, interacting, binding, covalent bonding, ionic bonding, hydrogen bonding, slowing the movement of, attesting, or any combinations thereof of the analyte complex as it enters and flows through the concentrating region. Other components of the sample fluid will continue to pass therethrough.
  • the immobilized material can include a ligand, gold, polymer, or a combination thereof that can be responsive with the analyte complex.
  • the affinity between the immobilized material and the analyte complex can be reversed by further modulating a charge of the analyte complex. This can occur as a charge-modifying fluid flows through the concentrating region and a pH of the fluid returns to neutral or as the divalent ion is removed.
  • the charge-modifying fluid can include an elution fluid or additional sample fluid.
  • the charge-modifying fluid may be added to the porous substrate of the self-wicking device at or upstream of the concentrating region.
  • the charge adjusting region and the concentrating region can overlap or be within the same physical area, but can provide both functions.
  • the detecting region can be configured to receive concentrated analyte from the concentrating region.
  • “Concentrated analyte” as used herein can refer to the analyte in any form and may include analyte complex, charge-modified analyte, analyte, or any other forms of analyte that are flowing through the detecting region.
  • the detecting region can include a test strip and a control strip.
  • the detecting region may be a sandwich format detecting region or a competitive format detecting region.
  • a sandwich format detecting region can generate a positive result by displaying an optical indicator, such as a colored line.
  • a competitive format detecting region can generate a positive result by displaying the absence of an optical indicator.
  • the self-wicking device may further include other components.
  • the device may include a fluid input port.
  • the fluid input port may be used to access the complexing region so that a sample fluid, an elution fluid, or a combination thereof may be applied through the fluid flow channel and onto the porous substrate.
  • the self-wicking device may also include an elution fluid input port.
  • the elution fluid input port may be positioned at or upstream of the concentrating region.
  • the elution fluid input port may also be used to add other charge-modifying fluids into the self-wicking device.
  • the self-wicking device can further include a flow controlling agent.
  • the flow controlling agent may be impregnated within the porous substrate and may include buffer salts, proteins, surfactants, and the like.
  • the self-wicking device may further include a housing.
  • a housing can be a casing that the porous substrate may be disposed within.
  • the housing may further include a viewing window over the detecting region. The viewing window may be an opening in the housing or may include an optically transparent material in the area of the detecting region to allow a user to view the results of a self-wicking test.
  • the self-wicking device can further include a backing. The backing can support the porous substrate.
  • the backing can include polyphenylene ether, polyester, polytetrafluoroethylene, glass, glass fiber, cellulose, nitrocellulose, or a combination thereof.
  • the backing can be used to provide stability to the porous substrate.
  • Self-wicking Systems [0023] Also presented herein is a self-wicking system 200.
  • the system can include a self-wicking device 100 and a sample fluid 210.
  • the self-wicking device can include a fluid flow channel including a porous substrate 110 for sequential fluid flow through multiple regions.
  • the multiple regions can include a complexing region 120 that can include a compound 122 having a functional group to bind with an analyte in a sample to form an analyte complex, a charge adjusting region 130 that can provide charge modulation by modifying a charge of the analyte complex or by providing a modified concentration of charged centers to interact with the analyte complex, a concentrating region 140 that can include an immobilized material 142 having an affinity to the analyte complex after charge modulation, and a detecting region 150 that can receive concentrated analyte from the concentrating region.
  • the charge adjusting region and the concentrating region can overlap or be within the same physical area, but can provide both functions.
  • the sample fluid 210 can include the analyte 212 to flow through the fluid flow channel.
  • the self-wicking device can be as described above.
  • the sample fluid may be a fluid that can include an analyte to be detected or can exclude the analyte to be detected by the self-wicking device. When the sample fluid includes the analyte, a positive result may be generated by the device. When the sample fluid excludes the analyte, a negative result may be generated by the device.
  • the analyte in the sample fluid may be selected from amino acids, peptide strands, glycans, polypeptides, antibodies, proteins, or a combination thereof. In one example, the analyte can include a protein.
  • the analyte may be at least ten residues long.
  • a single strand of the analyte may have a weight average molecular weight ranging from about 1,500 Daltons to about 250 KD, about 5,000 Daltons to about 200 KD, or from about 50 KD to about 250 KD.
  • the system can further include an elution fluid.
  • the elution fluid can decouple the analyte complex from the immobilized material in the concentrating region.
  • the sample fluid with the analyte complex can have a pH of from about 4 to about 6 and the elution fluid can be selected from a tris base, a dibasic sodium phosphate, magnesium oxide, disodium hydrogen phosphate, arginine, sodium bicarbonate, ammonium bicarbonate, sodium carbonate, borate, glycine-NaOH, or a combination thereof.
  • the sample fluid with the analyte complex can have a pH of from about 8 to about 10 and the elution fluid can be selected from citric acid, tris-HCl, monobasic sodium phosphate, tartaric acid, succinic acid, acetic acid, phosphoric acid, glycerine-HCl, sodium acetate, or a combination thereof.
  • the charge adjusting region, the concentrating region, or a combination thereof can include a divalent cation
  • the elution fluid can be selected from EDTA, EGTA, GLDA, trisodium ⁇ -DL-alanine diacetate, phytic acid, tetrasodium iminodisuccinate, or a combination thereof.
  • the method 300 can include flowing 310 a sample fluid along a fluid flow channel including a porous substrate, where relative to fluid flow, the fluid flow channel sequentially can include a complexing region, a charge adjusting region, a concentrating region, and a detecting region.
  • a sample fluid can pass sequentially through the complexing region, the charge adjusting region, the concentrating region, and the detecting region.
  • the method can further include binding 320 an analyte carried by the sample fluid to a compound having a functional group to bind with the analyte in the complexing region to form an analyte complex; modulating 330 a charge by modifying a charge of the analyte complex in the charge adjusting region or by providing a modification of charged centers in the charge adjusting region; immobilizing 340 the analyte complex in the concentrating region; releasing 350 concentrated analyte from the concentrating region; and detecting 360 the concentrated analyte in the detecting region.
  • modifying a charge of the analyte complex in the charge adjusting region can occur by modifying a pH of the sample fluid that the analyte complex is located in or by providing a divalent cation.
  • Modifying the charge by adjusting a pH can involve changing the pH of the sample fluid by from about 1 pH unit to about 3.5 pH units relative to a pH of the sample fluid in the complexing region.
  • the pH modifying fluids and divalent cations can be as described above.
  • the releasing can include, in some examples, passing an elution fluid through the concentrating region.
  • the elution fluid may vary depending on how the charge modulation occurs and can be as described above.
  • the detecting can include visually inspecting the detecting region for the presence of an optical indicator.
  • the optical indicator may be a colored dye in an example.
  • the method can concentrate an analyte when present in a sample fluid in the concentrating region of the device, thereby eliminating a need for sample preparation and concentrating steps before applying a sample fluid to a porous substrate of a self-wicking device. Further, concentrating the analyte in the concentrating region can improve the results of the device by increasing a quantity of the concentrated analyte passing through the detecting region at a time period thereby increasing an overall sensitivity of the device.
  • a range format is used merely for convenience and brevity and should be interpreted flexibly to include the numerical values explicitly recited as the limits of the range, and also to include all the individual numerical values or sub-ranges encompassed within that range as if individual numerical values and sub-ranges are explicitly recited.
  • a numeric range that ranges from about 10 to about 500 should be interpreted to include the explicitly recited sub-range of about 10 to about 500 as well as sub-ranges thereof such as about 50 and about 300, as well as sub-ranges such as from about 100 to about 400, from about 150 to about 450, from about 25 to about 250, etc.
  • the sample fluid is applied to a self-wicking device including a nitrocellulose porous substrate having a complexing region, a charge adjusting region, a concentrating region, and a detecting region thereon, as described above.
  • the complexing region includes a colored latex particle capable of binding with the protein to form an analyte complex.
  • a pH of the sample fluid including the analyte complex is reduced using monobasic sodium phosphate.
  • the analyte complex binds to immobilized gold in the concentrating region of the device.
  • a pH of the sample fluid is increased using dibasic sodium phosphate. Following an increase in the pH the analyte complex is released and passes to the detecting region, where a testing strip indicates the presence of the protein by showing a colored line.
  • Example 2 Antibody Concentration and Detection
  • a sample fluid is tested for the presence of an antibody.
  • the sample fluid is applied to a self-wicking device including a nitrocellulose porous substrate having a complexing region, a charge adjusting region, a concentrating region, and a detecting region thereon, as described above.
  • the complexing region includes a fluorescent latex particle capable of binding with the antibody to form an analyte complex.
  • Divalent calcium ions in the charge adjusting region provide additional interaction centers allowing the analyte complex to further interact and concentrate.
  • the analyte complex binds to immobilized gold in the concentrating region.
  • EDTA is flowed through the self-wicking device and the analyte complex is released from the concentrating region.
  • the analyte complex passes to the detecting region, where a testing strip indicates the presence of the antibody by showing a colored line.

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  • Health & Medical Sciences (AREA)
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  • Immunology (AREA)
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  • Urology & Nephrology (AREA)
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Abstract

La présente invention concerne un dispositif à auto-capillarité. Le dispositif peut comprendre un canal d'écoulement de fluide comprenant un substrat poreux pour un écoulement de fluide séquentiel à travers de multiples régions. Les multiples régions peuvent comprendre une région de complexation, une région d'ajustement de charge, une région de concentration et une région de détection. La région de complexation peut comprendre un composé ayant un groupe fonctionnel pour se lier avec un analyte dans un échantillon pour former un complexe d'analyte. La région d'ajustement de charge peut fournir une modulation de charge par la modification d'une charge du complexe d'analyte ou la fourniture d'une concentration modifiée de centres chargés pour interagir avec le complexe d'analyte. La région de concentration peut comprendre une matière immobilisée ayant une affinité pour le complexe d'analyte à la suite de la modulation de charge. La région de détection peut recevoir un analyte concentré provenant de la région de concentration.
PCT/US2020/054917 2020-10-09 2020-10-09 Dispositifs à auto-capillarité WO2022075992A1 (fr)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134796A1 (en) * 2004-12-17 2006-06-22 3M Innovative Properties Company Colorimetric sensors constructed of diacetylene materials
US8647888B2 (en) * 2010-10-01 2014-02-11 Hologic, Inc. Immunoassay test strip for use in a diagnostic system
EP2469269B1 (fr) * 2007-09-01 2016-03-30 Alere Switzerland GmbH Dispositif d'analyse avec des zones partagées
US10267792B2 (en) * 2016-09-09 2019-04-23 International Business Machines Corporation Device for detecting toxic shock syndrome toxins and method of making the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134796A1 (en) * 2004-12-17 2006-06-22 3M Innovative Properties Company Colorimetric sensors constructed of diacetylene materials
EP2469269B1 (fr) * 2007-09-01 2016-03-30 Alere Switzerland GmbH Dispositif d'analyse avec des zones partagées
US8647888B2 (en) * 2010-10-01 2014-02-11 Hologic, Inc. Immunoassay test strip for use in a diagnostic system
US10267792B2 (en) * 2016-09-09 2019-04-23 International Business Machines Corporation Device for detecting toxic shock syndrome toxins and method of making the same

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