WO2022075450A1 - Procédé d'inactivation de virus, dispositif d'inactivation de virus et dispositif de soufflerie pourvu d'un dispositif d'inactivation de virus - Google Patents

Procédé d'inactivation de virus, dispositif d'inactivation de virus et dispositif de soufflerie pourvu d'un dispositif d'inactivation de virus Download PDF

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WO2022075450A1
WO2022075450A1 PCT/JP2021/037339 JP2021037339W WO2022075450A1 WO 2022075450 A1 WO2022075450 A1 WO 2022075450A1 JP 2021037339 W JP2021037339 W JP 2021037339W WO 2022075450 A1 WO2022075450 A1 WO 2022075450A1
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virus
plant
waste
extract
light
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PCT/JP2021/037339
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English (en)
Japanese (ja)
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愛 塚田
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学校法人昭和大学
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Priority to CN202180053190.1A priority Critical patent/CN116056732A/zh
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/22—Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26—Accessories or devices or components used for biocidal treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/01—Deodorant compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00—Disinfection, sterilisation or deodorisation of air
    • A61L9/14—Disinfection, sterilisation or deodorisation of air using sprayed or atomised substances including air-liquid contact processes
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F24—HEATING; RANGES; VENTILATING
    • F24F—AIR-CONDITIONING; AIR-HUMIDIFICATION; VENTILATION; USE OF AIR CURRENTS FOR SCREENING
    • F24F7/00—Ventilation
    • F24F7/04—Ventilation with ducting systems, e.g. by double walls; with natural circulation
    • F24F7/06—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit
    • F24F7/08—Ventilation with ducting systems, e.g. by double walls; with natural circulation with forced air circulation, e.g. by fan positioning of a ventilator in or against a conduit with separate ducts for supplied and exhausted air with provisions for reversal of the input and output systems

Definitions

  • the present invention relates to, for example, a virus inactivating method for treating a virus existing in the air or a virus adhering to clothes, a virus inactivating device, and a blower device provided with the virus inactivating device.
  • viruses caused by viruses have a great impact on the entire world as an economic problem, not just a problem of health damage.
  • new coronavirus, influenza virus, norovirus, adenovirus, etc. infect humans and cause various symptoms such as fever, chills, diarrhea, headache, sore throat, joint / muscle pain, cough, and nasal discharge.
  • viruses that infect livestock animals such as bird flu and swine flu cause economic damage because it is necessary to kill the affected livestock animals because there are cases of infection to humans.
  • Patent Document 1 charged fine particle water containing radicals is generated by electrostatic atomization of water, and the charged fine particle water reacts with at least one of ammonia, acetaldehyde, and acetic acid, and among the ammonia, acetaldehyde, and acetic acid.
  • a technique for decomposing at least one and removing airborne bacteria is disclosed. The technique of Patent Document 1 is based on the method of decomposing an odorous component with ultrafine particle water disclosed in Patent Document 2 and the odorous component decomposing device.
  • Non-Patent Document 1 discloses a technique of generating active oxygen species by irradiating a waste extract in wine production with light and sterilizing bacteria and fungi with the generated active oxygen species.
  • white grape pomace and white wine starch are used as wastes in wine production, and the production of reactive oxygen species and antibacterial activity are confirmed in both cases.
  • the present invention is a virus inactivating method for effectively utilizing plant-derived waste such as waste in wine production and treating a virus existing in the air or a virus attached to the substance. It is an object of the present invention to provide a virus inactivating device and a blower equipped with the virus inactivating device.
  • the present invention includes the following.
  • Virus inactivation including a step of irradiating a plant-derived waste containing polyphenols or an extract thereof with light to generate a hydroxyl radical, and a step of inactivating a virus contained in a treatment target by the generated hydroxyl radical.
  • Activation method (2) The virus inactivating method according to (1), wherein the plant-derived waste is waste in wine production.
  • the virus inactivating method according to (1) wherein the plant-derived waste or its extract is irradiated with light at 10 to 1000 mW / cm 2 for 2 to 30 seconds.
  • the virus inactivating method of (1) which comprises spraying a solution containing a plant-derived waste or an extract thereof and irradiating the mist-like solution with light.
  • a radical generating unit comprising a plant-derived waste containing polyphenols or an extract thereof and generating a hydroxyl radical from the plant-derived waste or an extract thereof. It is provided with a light irradiation unit that irradiates the plant-derived waste or its extract of the radical generation unit with light.
  • a virus that generates a hydroxylated radical by irradiating the plant-derived waste or its extract of the radical generating section with light from the light irradiation section, and inactivates the virus contained in the treatment target by the generated hydroxyl radical. Inactivating device.
  • the virus inactivating device according to (6) wherein the plant-derived waste is waste in wine production.
  • the virus inactivating apparatus according to (7) wherein the waste in wine production is squeezed lees and / or starch after grape squeezing treatment.
  • the radical generating unit is provided with a spraying device for spraying a solution containing plant-derived waste or an extract thereof.
  • the virus inactivating device wherein the light irradiation unit irradiates a mist-like solution sprayed from the spray device with light.
  • a ventilation passage having an intake port and an exhaust port, and the virus inactivating device according to any one of (6) to (10) above are provided, and contact with hydroxylated radicals generated by the virus inactivating device.
  • the virus inactivating method according to the present invention it is possible to effectively inactivate a virus existing in the air or a virus attached to an object by a simple procedure.
  • the virus inactivating device according to the present invention can effectively inactivate a virus in the air or a virus attached to an object with a simple configuration.
  • the ventilation device according to the present invention is provided with the virus inactivating device, so that air in a state in which the existing virus is inactivated can be supplied from the exhaust port.
  • the virus inactivating method according to the present invention utilizes hydroxyl radicals generated by irradiating a plant-derived waste containing polyphenols or an extract thereof with light.
  • the plant-derived waste containing polyphenols is, for example, a residual component after squeezing a plant containing polyphenols (squeezed lees) and a residual component after distilling a plant containing polyphenols.
  • the residual components after solvent extraction treatment of plants containing polyphenols can be mentioned.
  • catechins catechin, galocatechin, epicatechin, epigalocatechin, catechin gallate, galocatechin gallate, epicatechin gallate, epigalocatechin gallate, etc.
  • catechin polymers flavonols (kercetin, mylicetin, etc.) Catechins, etc.) and their sugars; flavones (avigenin, chrysin, luteolin, etc.); isoflavones (diesechin, genistine, etc.) and their sugars; flabanones (naringenin, hesperetin, etc.) and their sugars; Examples thereof include anthocyanidins (cianidine, delphinidin, pelargonidin and the like) and sugar catechins thereof; catechins; curcumins; lignans; coumarins; phenylcarboxylic acids and the like.
  • Plants containing polyphenols are not particularly limited, but are plants containing anthocyanins such as grapes, purple potatoes, blueberries, bilberries, mulberries, blackberries, hascups and cassis, and plants containing catechins such as green tea, tea and oolong tea.
  • Plants containing cacao polyphenols such as cacao, plants containing rutin such as Dattan buckwheat and buckwheat of the family Tade, plants containing curcumin such as turmeric, Dutch biu, soybeans, sprouts, yaenari sprouts, kudzu roots, purple thorns and purple sprouts.
  • Examples of plants containing isoflavone plants containing chlorogenic acids (coffee polyphenols) such as coffee, and plants containing other polyphenols such as ferulic acid, tannin, ellagic acid, lignan or coumarin.
  • the plant containing polyphenol it is preferable to use a plant containing anthocyanin, particularly a fruit containing anthocyanin.
  • a plant containing anthocyanin particularly a fruit containing anthocyanin.
  • grapes are particularly preferable. That is, in the present invention, as the plant-derived waste containing polyphenols, it is preferable to use the waste obtained by using grapes.
  • anthocyanin-containing pericarp and the like are waste.
  • the waste in wine production may contain fruit stems, seeds and the like after de-stemming. In wine production, the sediment that has settled on the bottom of the tank becomes waste.
  • waste in wine production it is preferable to use waste in wine production. That is, as the plant-derived waste containing polyphenols, it is preferable to use the squeezed lees and / or the starch after the grape squeezing treatment.
  • the extract of plant-derived waste containing polyphenols is a residual component (squeezed cake) after pressing a plant containing polyphenols, and after distilling a plant containing polyphenols.
  • the solvent for extracting the polyphenol component include water and a water-containing organic solvent.
  • a solution obtained by immersing the squeezed lees produced in wine production after the grape squeezing treatment or the sediment settled at the bottom of the wine tank in an appropriate solvent and extracting the polyphenol component contained in the squeezed lees or the starch is used. Can be done.
  • grape varieties used in wine production include, for example, Chardonnay, Semillon, Riesling, Sauvignon Blanc, Muller Turgau, Muscat, Verdele, Koshu, Traminer, Delaware, Niagara and other white wines. Grape varieties can be mentioned. Examples of grape varieties for red wine include Cabernet Sauvignon, Cabernet Franc, Merlot, Pinot Noir, Syrah, Alicant, Nebbiolo, Muscat Berry A, and Black Queen.
  • the present invention is not limited to the above-mentioned grape-derived waste as plant-derived waste containing polyphenols, and for example, Rutaceae citrus (Rutaceae) such as lime, lemon, grapefruit, summer orange, iyokan, wenshu orange, and poncan. (Genus); Rutaceae, such as Nagakinkan and Malkinkann, Grapevine; Grapevine, such as grapes; Vaccinium, such as blueberries and moss; Waste can also be used.
  • Rutaceae citrus such as lime, lemon, grapefruit, summer orange, iyokan, wenshu orange, and poncan.
  • Rutaceae such as Nagakinkan and Malkinkann
  • Grapevine Grapevine
  • Vaccinium such as blueberries and moss
  • Waste can also be used.
  • plant-derived waste containing polyphenols one type of plant-derived waste may be used, or two or more types of plant-derived waste may be used.
  • plant-derived waste containing polyphenols grape lees and coffee beans after extraction treatment can be used in combination.
  • a hydroxyl radical generated by irradiating a plant-derived waste containing polyphenols or an extract thereof with light acts on the virus to inactivate the virus.
  • inactivating a virus means an effect of removing or reducing the infectious or proliferating ability of the virus.
  • the virus to be processed is not particularly limited, and includes both enveloped type and non-enveloped type.
  • the virus to be processed is any of double-stranded DNA virus, single-stranded DNA virus, double-stranded RNA virus, single-stranded (+) RNA virus, single-stranded (-) RNA virus, and reverse transcription virus. You may.
  • viruses belonging to the family Caliciviridae, Orthomyxoviridae, Coronaviridae, Herpesviruses, and the like examples include viruses belonging to the genus Norovirus, Sapovirus, Lagovirus, Nebovirus and Vesivirus.
  • viruses belonging to the orthomixovirus family examples include viruses belonging to the genus A influenza virus, the genus B influenza virus, the genus C influenza virus, the genus Togotovirus, and the genus Aisavirus.
  • the coronaviridae includes the subfamily Letovirinae and the subfamily Orthocoronavirus.
  • viruses belonging to the subfamily Retrovirus of the family Coronaviridae include viruses belonging to the genus Alphaletovirus.
  • examples of the virus belonging to the ortho-coronavirus subfamily of the coronavirus family include viruses belonging to the genus Alpha Coronavirus, Beta Coronavirus, Delta Coronavirus, and Gamma Coronavirus.
  • examples of the virus belonging to the herpesvirus family include viruses belonging to the genus Simplevirus, Variserovirus, Lymphocryptvirus, Cytomegalovirus, Roseolovirus and Radinovirus.
  • the light to irradiate the plant-derived waste containing polyphenols or its extract can be arbitrarily determined from the wavelength range of visible light of 360 nm to 830 nm. It is preferable to use shorter wavelength visible light in order to provide higher energy. For example, it can be visible light of 360 to 450 nm, preferably 360 to 400 nm visible light, and more preferably 360 to 380 nm visible light.
  • the light irradiating the plant-derived waste containing polyphenols or an extract thereof may have an irradiance and an irradiation time sufficient to generate hydroxylated radicals from the waste or the extract thereof.
  • the irradiance of 10 to 1000 [mW / cm 2 ] can be set to 2 to 30 [seconds]
  • the irradiance of 50 to 100 [mW / cm 2 ] can be set to 2 to 30 [seconds].
  • the irradiance of 100 [mW / cm 2 ] can be set to 15 to 30 [seconds]
  • the irradiance of 50 [mW / cm 2 ] can be set to 2 to 6 [seconds].
  • a hydroxyl radical is generated by irradiating a plant-derived waste containing polyphenols or an extract thereof with light, thereby inactivating the virus. do.
  • Virus inactivation can be quantitatively evaluated by, for example, the TCID 50 (Median tissue culture infectious dose) method or the plaque method. According to the virus inactivation method according to the present invention, when the amount of virus (the amount of infectious virus) is measured by the TCID 50 method, it can be statistically significantly reduced as compared with the untreated virus.
  • the amount of virus can be reduced by at least 10%, the amount of virus can be reduced by at least 20%, the amount of virus can be reduced by at least 30%, and the amount of virus can be reduced by at least 40%. Can reduce the amount of virus by at least 50%, reduce the amount of virus by at least 60%, reduce the amount of virus by at least 70%, reduce the amount of virus by at least 80%. Alternatively, the amount of virus can be reduced by at least 90%.
  • the virus inactivating method according to the present invention described above can be realized as, for example, the virus inactivating device 1 as shown in FIG.
  • the virus inactivating device 1 shown in FIG. 1 includes a waste holding unit 2 (radical generating unit) including a plant-derived waste containing polyphenol or an extract thereof, and a plant-derived waste held in the waste holding unit 2. It is provided with a light irradiation unit 3 that irradiates an object or an extract thereof with light having a predetermined wavelength and irradiance.
  • the waste holding unit 2 may be a frame filled with the above-mentioned plant-derived waste, or a non-woven fabric or woven fabric impregnated with an extract of the plant-derived waste. can. Further, in the virus inactivating device 1, it is preferable that the waste holding portion 2 is detachably arranged.
  • the light irradiating unit 3 irradiates the waste holding unit 2 with light having a predetermined wavelength and irradiance.
  • hydroxide radicals are generated from the polyphenol component contained in the plant-derived waste or its extract in the space 4 between the waste holding portion 2 and the light irradiation portion 3. Therefore, the virus can be inactivated in the space 4 by introducing a gas containing the virus into the space 4.
  • the waste or the like holding portion 2 when the waste or the like holding portion 2 is detachable in the virus inactivating device 1, the waste or the like holding portion 2 can be replaced when the amount of hydroxylated radicals generated decreases. As a result, it is possible to prevent a decrease in the amount of hydroxyl radicals generated and permanently treat the gas containing the virus.
  • the virus inactivating device 1 to which the present invention is applied is not limited to the configuration shown in FIG. 1, and as shown in FIG. 2, instead of the waste holding unit 2, plant-derived waste containing polyphenols or It may be provided with a spraying device 5 for spraying the extract.
  • the spraying device 5 includes a tank 6 filled with a solution containing plant-derived waste or an extraction solution of plant-derived waste, and a spray nozzle 8 connected to the tank 6 via a pipe member 7. .
  • the tank 6 can be additionally filled with a solution containing plant-derived waste or an extraction solution of plant-derived waste.
  • the extract of plant-derived waste can be sprayed from the spraying device 5. Then, the virus inactivating device 1 irradiates the sprayed extract of plant-derived waste with light having a predetermined wavelength and irradiance from the light irradiation unit 3. As a result, hydroxide radicals are generated from the polyphenol component contained in the extract sprayed into the space 4 between the waste holding portion 2 and the light irradiation portion 3. Therefore, the virus can be inactivated in the space 4 by introducing a gas containing the virus into the space 4.
  • the virus inactivating device 1 shown in FIG. 2 since the solution containing the plant-derived waste or the extraction solution of the plant-derived waste is sprayed by the spraying device 5, the hydroxylated radical is sprayed in a relatively wide range (space 4). ), And can treat a wide range of gas containing viruses.
  • the tank 6 since the tank 6 can be additionally filled with the solution containing the plant-derived waste or the extraction solution of the plant-derived waste, the gas containing the virus can be additionally filled for a long period of time. Can be processed over.
  • the virus inactivating device 1 to which the present invention is applied can inactivate the virus contained in the gas by the hydroxyl radical generated from the plant-derived waste containing polyphenols or the extract thereof. Therefore, the virus in the air can be inactivated by applying the virus inactivating device 1 to the ventilation device.
  • the ventilation device 10 provided with the virus inactivating device 1 has an intake port 11 for taking in ambient air and an exhaust port 12 for discharging the treated air in the middle of the ventilation path 13.
  • the virus inactivating device 1 can be arranged in the air.
  • the ventilation device 10 is not limited to the size of the device and other components, and includes any device that allows air to pass through the ventilation passage 13 and supplies air from the exhaust port 12.
  • the ventilation device 10 examples include an air conditioner (air conditioner) that supplies air set to a desired temperature, an air purifier that removes fine particles in the air, a hair dryer, a dryer, and circulation of air in the device. Blowers and the like can be mentioned. Therefore, although not shown, the ventilation device 10 can be provided with a fan, a motor, a temperature control device (cooling device or heater), a filter, or the like as means for blowing air.
  • air conditioner air conditioner
  • the ventilation device 10 can be provided with a fan, a motor, a temperature control device (cooling device or heater), a filter, or the like as means for blowing air.
  • the ventilation device 10 configured in this way, the air introduced from the intake port 11 is exposed to the hydroxyl radical in the virus inactivating device 1, and the virus contained in the air can be inactivated. Therefore, the ventilation device 10 can supply the virus-inactivated air from the exhaust port 12. More specifically, as shown in FIG. 4, the ventilation device 10 can include the virus inactivating device 1 shown in FIG. In this case, the space 4 is irradiated with light under predetermined conditions for the mist-like extract of plant-derived waste, and the hydroxyl radical generated by this is present. Then, the air introduced from the intake port 11 is exposed to the hydroxide radical in the space 4. Then, the air inactivated by the virus is discharged from the exhaust port 12.
  • the ventilation device 10 may be provided with a filter 14 between the virus inactivating device 1 and the exhaust port 12.
  • the filter 14 may be, for example, one filled with the above-mentioned plant-derived waste in a frame, or a non-woven fabric or woven fabric impregnated with an extract of the plant-derived waste.
  • the filter 14 contains the above-mentioned polyphenol-containing plant-derived waste and its extract, residual hydroxide radicals can be eliminated.
  • the virus inactivating device 1 described above can be applied not only to the ventilation device 10 but also to the decontamination device 20 as shown in FIG.
  • the decontamination device 20 decontaminates all objects to be treated 21 that are considered to be contaminated by a virus, such as masks, medical goggles, and clothes of medical workers.
  • the decontamination device 20 carries a virus inactivating device 1 having a spraying device 5, a light irradiation unit 3 that irradiates a light irradiation unit 3 to irradiate an extraction solution of plant-derived waste sprayed on the space 4, and an object to be treated. It is provided with a mounting table 22 for placing.
  • the decontamination device 20 configured as described above can decontaminate the object to be processed 21 placed on the mounting table 22 by the hydroxide radical generated in the space 4. That is, the virus inactivating device 1 can not only inactivate the virus in the air, but also inactivate the virus attached to the object to be treated. At this time, since the polyphenol component contained in the extracted solution of the sprayed plant-derived waste has an antioxidant effect unlike the hydroxyl radical, the functionality due to the antioxidant effect of the polyphenol component is added to the object to be treated. can do.
  • Example 1 In this example, it was found that when polyphenols are irradiated with light under predetermined conditions, hydroxyl radicals are generated via hydrogen peroxide (Tsukada M, Nakashima T, Kamachi T, Niwano Y. Prooxidative potential of photo-irradiated aqueous extracts. Based on PLoS One, 11 (6): e0158197, 2016), we examined whether the hydroxyl radicals generated in this way could inactivate the virus.
  • influenza virus and norovirus were treated.
  • Influenza virus was infected with MDCK cells (derived from canine kidney cells), and norovirus was infected with RAW264.7 cells (derived from mouse macrophages) and cultured.
  • the amount of virus was 3 ⁇ 10 5/100 ⁇ L / well.
  • the polyphenol-containing sample and the virus solution were mixed at a ratio of 1:10, irradiated with ultraviolet light (405 nm, 20 W) at 0, 15, 30 and 120 seconds, and then the amount of virus reduction was quantified by the TCID 50 method. ..
  • the polyphenol-containing sample uses a grape peel water extract (polyphenol amount; 760 ⁇ g / mL) and a persimmon water extract (polyphenol amount; 390 ⁇ g / mL), and the control is water as an extraction solvent. And said.
  • Example 2 In this example, the amount of hydrogen peroxide generated when the grape peel water extract and the persimmon water extract used in Example 1 were irradiated with light was measured.
  • Example 1 ⁇ Experimental method> The conditions of light irradiation for 15 seconds and 30 seconds for the grape peel water extract and the persimmon water extract for which the effect of the virus inactivation test was obtained in Example 1 were verified. First, diluted products 5 times and 10 times the stock solution concentration were prepared for each sample, and irradiated with light (402 nm, 20 W) at the above time. After irradiation, the principle commonly used to measure the amount of hydrogen peroxide (quantified based on the formation of a complex of xylinol orange and ferric (produced by peroxide-dependent oxidation of ferric ions)). The amount of hydrogen peroxide was quantified. A 2-fold diluted series product with a hydrogen peroxide concentration of 10 ⁇ M was used as the standard for the calibration curve.
  • Example 1 As shown in Table 1, as a result of Example 1, the persimmon-derived polyphenol-containing extract tended to have higher inactivation activity than the grape-derived polyphenol-containing extract, but the test results of this example support this. It became. In addition, the efficacy of gallic acid (single polyphenol) tends to decrease with long-term irradiation, but when a plant-derived extract that is thought to contain various polyphenols is used, hydroxylation radicals are generated over a long period of time. It was possible to consider that. All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.

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  • Inorganic Chemistry (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention inactive les virus dans l'air et les virus présents sur un objet en tant que cible à traiter. Dans la présente invention, des déchets d'origine végétale contenant des polyphénols ou un extrait de ceux-ci sont irradiés avec de la lumière pour générer des radicaux hydroxyles et les radicaux hydroxyles générés inactivent des virus présents dans un objet à traiter.
PCT/JP2021/037339 2020-10-09 2021-10-08 Procédé d'inactivation de virus, dispositif d'inactivation de virus et dispositif de soufflerie pourvu d'un dispositif d'inactivation de virus WO2022075450A1 (fr)

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JPS57177324A (en) * 1981-04-24 1982-11-01 Mitsubishi Electric Corp Air purifier
JP2000210521A (ja) * 1999-01-20 2000-08-02 Cosmo Ace:Kk 気体清浄化方法及び気体清浄機
WO2012060450A1 (fr) * 2010-11-05 2012-05-10 国立大学法人徳島大学 Procédé de stérilisation de fruits et de légumes
CN103656719A (zh) * 2013-12-19 2014-03-26 刘传林 羟基自由基对室内空气消毒解毒净化的方法及其装置
JP2017172068A (ja) * 2016-03-23 2017-09-28 入江　敏夫 基材−シリカゾル乾燥物複合体、その応用物品及びその製造方法
JP2018023977A (ja) * 2017-11-02 2018-02-15 国立研究開発法人農業・食品産業技術総合研究機構 還元性有機物を利用した光触媒

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JPS57177324A (en) * 1981-04-24 1982-11-01 Mitsubishi Electric Corp Air purifier
JP2000210521A (ja) * 1999-01-20 2000-08-02 Cosmo Ace:Kk 気体清浄化方法及び気体清浄機
WO2012060450A1 (fr) * 2010-11-05 2012-05-10 国立大学法人徳島大学 Procédé de stérilisation de fruits et de légumes
CN103656719A (zh) * 2013-12-19 2014-03-26 刘传林 羟基自由基对室内空气消毒解毒净化的方法及其装置
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