WO2022050384A1 - Système de dosage - Google Patents

Système de dosage Download PDF

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Publication number
WO2022050384A1
WO2022050384A1 PCT/JP2021/032467 JP2021032467W WO2022050384A1 WO 2022050384 A1 WO2022050384 A1 WO 2022050384A1 JP 2021032467 W JP2021032467 W JP 2021032467W WO 2022050384 A1 WO2022050384 A1 WO 2022050384A1
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WO
WIPO (PCT)
Prior art keywords
container
assay
liquid
containers
main body
Prior art date
Application number
PCT/JP2021/032467
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English (en)
Japanese (ja)
Inventor
雄介 渕脇
正人 田中
昌平 山村
直樹 森下
久美子 神谷
誠一郎 松▲崎▼
Original Assignee
国立研究開発法人産業技術総合研究所
日本ハム株式会社
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Application filed by 国立研究開発法人産業技術総合研究所, 日本ハム株式会社 filed Critical 国立研究開発法人産業技術総合研究所
Priority to JP2022546985A priority Critical patent/JPWO2022050384A1/ja
Publication of WO2022050384A1 publication Critical patent/WO2022050384A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass

Definitions

  • the present invention relates to an assay system comprising an assay apparatus configured to perform an assay using a liquid.
  • ⁇ l (microliter) order that is, trace reagents, treatment agents, and substrates of about 1 ⁇ l or more and less than about 1 ml (milliliter).
  • An operation for accurately supplying a liquid such as a solution to the assay device is required.
  • technologies such as POCT (Point Of Care Testing) that rapidly perform tests, diagnoses, assays, etc. are attracting attention.
  • An operation of accurately and easily supplying liquids such as various kinds of reagents to the assay device is required.
  • pipette operation For example, as an operation of supplying a liquid, an operation of manually supplying a liquid to an assay device using a pipette such as a micropipette (hereinafter referred to as “pipette operation”) can be mentioned.
  • pipette operation requires accuracy, it is difficult and complicated. That is, the handling of the liquid is not easy.
  • Such pipette operations need to be performed by a limited number of skilled technicians, which causes a shortage of personnel in the field.
  • manual pipette operation has problems such as a risk of contamination between reagents and an increase in preparation time.
  • an operation of automatically supplying the liquid an operation of automatically supplying the liquid stored in a container such as a tank to the assay device by using a pump (hereinafter referred to as "pump operation") can also be mentioned.
  • pump operation an operation of automatically supplying the liquid stored in a container such as a tank to the assay device by using a pump
  • a high-performance material such as sterilized plastic for the container and the piping for sending the liquid from this container to the pump, and the liquid is automatically supplied from the tank by the pump.
  • the configuration for this is complicated.
  • the device for performing such a pump operation becomes expensive.
  • such an apparatus becomes complicated because it has a tank, a pump, and the like.
  • a plurality of fluid transport means each having an actuator, a plurality of fluid passages connected to each of the plurality of fluid transport means, and at least one obtained by merging the plurality of fluid passages.
  • a microfluidic cartridge comprising at least one merging passage extending from a merging point, wherein the actuator of each fluid transport means is arranged on the fluid passage connected to the fluid transport means, and reagents and the like in the fluid passage. It has a piston that reciprocates in a pressurable manner so as to send the fluid of There is a microfluidic cartridge.
  • an assay technique is a liquid packaging component with multiple liquid storage sections for packaging liquids such as reagents, and multiple burstable seals each covering each of these multiple liquid storage compartments, and each burstable.
  • An assay system that includes a seal rupture component configured to be able to rupture a single seal and an assay device configured to receive liquid from multiple liquid storage sections, with each seal rupturing component being an assay device. Included is an assay system that includes a plunger that compresses a liquid storage compartment covered by its seal so that it is stripped to rupture. (See, for example, Patent Document 2.)
  • an assay technique is an upper treatment chamber configured to perform a bioassay and a lower treatment chamber located below the upper treatment chamber and configured to perform the bioassay.
  • These upper and lower treatment chambers are arranged so as to be separated in the vertical direction, a plurality of reagent containers that fluidly communicate with the upper treatment chamber by the capillary channel, and fluid communication with the lower treatment chamber by the capillary channel.
  • a cartridge with an additional chamber so that when the difference in pressure applied to the upper and lower treatment chambers exceeds the critical value, the fluid enters the lower treatment chamber from the upper treatment chamber through the porous substrate.
  • Cartridges are configured such that the transport of fluid between chambers that are fluid-communicated by the capillary channels is controlled by applying air pressure that exceeds the capillary pressure resistance between these chambers. (See, for example, Patent Document 3.)
  • the assay technique described above when attempting to increase the number of reagents handled, the number of fluid passages containing the reagents increases, which complicates the arrangement structure of the fluid passages, resulting in a microfluidic cartridge. The structure of is complicated. Also in another example of the assay technique described above, when attempting to increase the number of reagents to be handled, the number of liquid storage sections containing the reagents increases, which complicates the arrangement structure of the liquid storage sections. As a result, the structure of the liquid package component is complicated. This makes it difficult to perform assays for various protocols.
  • the assay system is an assay system including an assay device configured to perform an assay using a liquid, so that the liquid can be dropped onto the assay device.
  • a liquid supply device configured, a moving device configured to allow the assay device to move relative to the liquid supply device in the horizontal direction, a liquid drop by the liquid supply device, and the movement.
  • the liquid supply device comprises a plurality of containers, each configured in the form of an instillation container, comprising a control device configured to control the movement of the assay device by the device, where each container contains a liquid.
  • the liquid supply device includes a main body portion formed so as to be accommodating, and a nozzle portion having a discharge port configured to allow the liquid contained in the main body portion to be dropped.
  • a container mounting mechanism configured to allow the plurality of containers to be mounted in a state where the discharge ports of the containers are oriented downward and spaced apart from each other in the horizontal direction, and a liquid is dropped from the discharge ports of the plurality of containers.
  • the control device includes a container pushing mechanism having at least one movable member configured to be able to push the main body portion of the plurality of containers, and the control device includes the plurality of containers in which the at least one movable member has the plurality of movable members. It is configured to control the container pushing mechanism in order to push the main body of the container in a desired order and timing.
  • a plurality of liquids can be supplied quickly and continuously, a small amount of liquid can be supplied with high accuracy, and the configuration for supplying the liquid can be simplified. It can reduce the risk of contamination.
  • FIG. 1 is a perspective view schematically showing the assay system according to the first embodiment.
  • FIG. 2 is a block diagram of the assay system according to the first embodiment.
  • FIG. 3 is a perspective view schematically showing a liquid supply device, a mobile device, an assay device, and peripheral portions of the assay system according to the first embodiment.
  • FIG. 4 is a perspective view schematically showing a container holder of the container mounting mechanism of the liquid supply device according to the first embodiment and a container housed therein.
  • FIG. 5 is a cross-sectional view taken along the line AA of FIG.
  • FIG. 6A schematically shows the container holder of the container mounting mechanism according to the first embodiment, the container housed therein, and the container pushing mechanism in the advanced state so as to be cut along the line BB of FIG.
  • FIG. 6B schematically shows the container holder of the container mounting mechanism according to the first embodiment, the container housed therein, and the container pushing mechanism in the retracted state so as to be cut along the line BB of FIG.
  • FIG. 7 is a side view schematically showing a nozzle portion of a container and a liquid detection device of the assay system according to the first embodiment.
  • FIG. 8 is a plan view schematically showing an assay device of the assay system according to the first embodiment.
  • FIG. 9 is a cross-sectional view taken along the line CC of FIG.
  • FIG. 10 is a perspective view schematically showing a state in which the measuring device of the assay system according to the first embodiment is measuring the assay device.
  • FIG. 11 is a plan view schematically showing the assay system according to the second embodiment.
  • FIG. 12 is a block diagram of the assay system according to the second embodiment.
  • FIG. 13 is a cross-sectional view taken along the line DD of FIG.
  • the assay system according to the first and second embodiments will be described.
  • the assay system according to each embodiment includes an assay device configured to perform an assay using a liquid.
  • the liquid applicable to the assay device according to the present embodiment is not particularly limited as long as it can flow in the assay device.
  • Liquids applicable to such assay devices can include not only chemically pure liquids, but also gases, other liquids or solids dissolved, dispersed or suspended in liquids.
  • the liquid may be hydrophilic, and the hydrophilic liquid may be, for example, human or animal whole blood, serum, plasma, blood cells, urine, fecal diluent, saliva, sweat, tears, nail extract, etc. Examples thereof include a liquid sample derived from a living body such as a skin extract, a hair extract, or a cerebrospinal fluid.
  • the liquid when the liquid is a reagent used at the time of assay, the liquid includes buffer solution, general biochemical reagent, immunochemistry-related reagent, antibody-related reagent, peptide solution, protein / enzyme-related reagent, cell-related reagent, etc.
  • the liquid is not limited to these.
  • an in vitro diagnostic drug for pregnancy test, urine test, stool test, adult disease test, allergy test, infectious disease test, drug test, cancer test, etc. general-purpose test drug, POCT It is possible to measure a sample that is effective for clinical examination, diagnosis, or analysis in a liquid sample for applications such as (Point Of Care Testing), but the application of the assay device is not particularly limited.
  • the hydrophilic liquid is not limited to biological samples, and includes, for example, food suspensions, food extracts, production line wash water, wiping liquid, drinking water, river water, soil suspensions, and the like. Can be mentioned.
  • the assay device may measure pathogens in food or drinking water, or pollutants in river water or soil.
  • These hydrophilic liquids may typically use water as a solvent, and may be any aqueous solution that can be exchanged by an assay device.
  • lateral flow refers to the flow of liquid that moves due to the driving force of gravitational sedimentation.
  • the movement of the liquid based on the lateral flow refers to the movement of the liquid in which the driving force of the liquid due to gravitational sedimentation acts predominantly (dominantly).
  • the movement of the liquid based on the capillary force refers to the movement of the liquid in which the interfacial tension acts predominantly (predominantly).
  • the movement of liquid based on lateral flow is different from the movement of liquid based on capillary force.
  • specimen refers to a compound or composition that is present in a liquid and is detected or measured.
  • the specimen may be a saccharide (eg, glucose), a protein or peptide (eg, a serum protein, a hormone, an enzyme, an immunomodulator, a phosphokine, a monokine, a cytokine, a glycoprotein, a vaccine antigen, an antibody, a growth factor, or a growth factor).
  • a saccharide eg, glucose
  • protein or peptide eg, a serum protein, a hormone, an enzyme, an immunomodulator, a phosphokine, a monokine, a cytokine, a glycoprotein, a vaccine antigen, an antibody, a growth factor, or a growth factor.
  • Fats amino acids, nucleic acids, cells, steroids, vitamins, pathogens or antigens thereof, natural or synthetic chemicals, contaminants, therapeutic drugs or illegal drugs or toxicants, or metabolites or antibodies of these substances. It is good to
  • the "reference substance" is a known substance different from the sample, which is added to the liquid in a known amount for detecting the sample concentration.
  • the reference substance can be selected from the above options in the same manner as the sample, and can be selected in relation to the sample. In particular, it does not interact with the sample and can be selected from stable substances.
  • a "microchannel” is meant to detect or measure a specimen on the order of ⁇ l (microliter), i.e., with a trace amount of liquid greater than or equal to about 0.1 ⁇ l and less than about 1 ml (milliliter).
  • a trace amount of liquid greater than or equal to about 0.1 ⁇ l and less than about 1 ml (milliliter).
  • film refers to a film-like object or a plate-like object having a thickness of about 200 ⁇ m (micrometer) or less
  • sheet refers to a film-like object or a film-like object having a thickness of more than about 200 ⁇ m. Refers to a plate-like object.
  • plastic refers to a polymerizable material or a polymer material polymerized or molded so as to be used as an essential component. Plastics also include polymer alloys that combine two or more polymers.
  • the "porous medium” may be a member having a plurality of and a large number of micropores and capable of sucking and passing a liquid, or a member capable of capturing or concentrating a solid substance, and may be a paper.
  • the porous medium may be hydrophilic when the liquid is hydrophilic, and may be hydrophobic when the liquid is hydrophobic.
  • the porous medium may be hydrophilic and may be paper, cotton wool or the like containing a large number of fibers.
  • the porous medium can be one or more of cellulose, nitrocellulose, cellulose acetate, filter paper, tissue paper, toilet paper, paper towels, fabrics, cotton, or water-permeable hydrophilic porous polymers. ..
  • a liquid content such as a liquid eye drop contained in the main body is dropped drop by drop from the ejection port of the nozzle portion (in the case of the case).
  • the eye drop container is not a so-called single-use type, but a so-called bottle type.
  • the eye drop container is made of an elastically deformable material so that the main body thereof can be returned to its original shape after being pushed.
  • the eye drop container is preferably made of plastic.
  • the assay system includes an assay device 10 configured to perform an assay using liquid L.
  • the assay system includes a liquid supply device 20 configured to allow the liquid L to be dropped onto the assay device 10.
  • the assay system includes a mobile device 30 configured to allow the assay device 10 to move horizontally relative to the liquid supply device 20.
  • the assay system includes a control device 40 configured to be able to control the dropping of the liquid L by the liquid supply device 20 and the movement of the assay device 10 by the moving device 30.
  • the liquid supply device 20 includes a plurality of containers 21 each configured in the form of an eye drop container.
  • Each container 21 includes a main body portion 21a formed so as to be able to accommodate the liquid L.
  • Each container 21 includes a nozzle portion 21b having a discharge port 21c configured to allow the liquid L contained in the main body portion 21a to be dropped.
  • the main body portion 21a and the nozzle portion 21b can be attached to each other by fastening screws.
  • the liquid supply device 20 is configured so that a plurality of containers 21 can be attached with their discharge ports 21c facing downward and horizontally spaced apart from each other.
  • the container mounting mechanism 22 is included.
  • the liquid supply device 20 may push the main body 21a of the plurality of containers 21 in order to drop the liquid L from the discharge ports 21c of the plurality of containers 21.
  • the control device 40 is configured to control the container pushing mechanism 23 so that at least one movable member 23a pushes the main body 21a of the plurality of containers 21 in a desired order and timing.
  • the assay system according to the present embodiment can be roughly configured as follows.
  • the container mounting mechanism 22 has a plurality of container holders 24 for holding a plurality of containers 21, respectively.
  • Each container holder 24 has a main body accommodating portion 24a that receives the main body portion 21a of the container 21 held therein.
  • Each container holder 24 has a nozzle holding portion 24b that holds the nozzle portion 21b of the container 21 held therein.
  • the main body accommodating portion 24a of each container holder 24 has a passage port 24c that opens so as to allow passage of the movable member 23a that pushes the main body portion 21a of the container 21 accommodated therein.
  • the main body accommodating portion 24a of each container holder 24 is formed so as to receive the main body portion 21a of the container 21 pushed by the movable member 23a passing through the passage port 24c. It has a receiving portion 24d.
  • the assay system includes a liquid detection device 50 configured to be able to separately detect the liquid L dropped from the discharge port 21c of the nozzle portion 21b in the plurality of containers 21.
  • the control device 40 pushes the main body 21a of the container 21 when the liquid detection device 50 detects the liquid L dropped from the discharge port 21c of each container 21. It is configured to control the container pushing mechanism 23 in order to return the main body portion 21a from the state to the retracted state in which the main body portion 21a is not pushed.
  • the assay system comprises a measuring device 60 configured to be capable of measuring the reaction obtained in the assay device 10 by the liquid L dropped from the liquid supply device 20.
  • the measuring device 60 is arranged horizontally apart from the liquid supply device 20.
  • the control device 40 feeds the assay device 10 from the drop section (shown in FIG. 3) to the measurement section (shown in FIG. 10). It is configured to control the moving device 30 in order to move it horizontally relative to the device 20 and the measuring device 60.
  • the movement of the assay device 10 can be performed when the container pushing mechanism 23 changes from the advanced state (shown in FIG. 6A) to the retracted state (shown in FIG. 6B).
  • the dropping section is a section in which the liquid L is dropped from the liquid supply device 20 to the assay device 10. In FIG. 3, the dropping section is located below the liquid supply device 20.
  • the measurement section is a section in which the measuring device 60 measures the reaction obtained by the assay device 10 by the liquid L dropped in the dropping section.
  • the assay device 10 has a plurality of assay modules 11 configured to obtain a reaction by the liquid L dropped from the liquid supply device 20.
  • FIG. 8 shows, as an example, an assay device 10 having six assay modules 11.
  • the number of assay modules in the assay device can be 2-5 or 7 or more.
  • the assay device can also have only one assay module.
  • the liquid supply device 20 is configured to allow the liquid L to be dropped onto the plurality of assay modules 11 in a desired order and timing.
  • the measuring device 60 is configured to be capable of measuring the reactions obtained by the plurality of assay modules 11 by the liquid L dropped from the liquid supply device 20 in a desired order and timing.
  • the control device 40 sets the order and timing for measuring the reaction of the plurality of assay modules 11 by the measuring device 60 in the order in which the liquid L is dropped from the liquid supply device 20 to the plurality of assay modules 11.
  • the liquid supply device 20, the moving device 30, and the measuring device 60 are configured to control the liquid supply device 20, the moving device 30, and the measuring device 60 so as to be the same with respect to the timing at regular time intervals.
  • the container pushing mechanism 23 has a plurality of movable members 23a arranged so as to be able to push the main body portions 21a of the plurality of containers 21 respectively.
  • the mobile device 30 is configured to be able to move the assay device 10 along a predetermined movement path, as indicated by the double-sided arrows R.
  • the plurality of containers 21 are arranged so that the discharge ports 21c of the nozzle portions 21b are arranged along the movement path of the assay device 10.
  • control device 40 is electrically connected to the liquid supply device 20, the moving device 30, the liquid detection device 50, and the measuring device 60.
  • the control device 40 is configured to control the container pushing mechanism 23 so that the plurality of movable members 23a push the main body portions 21a of the plurality of containers 21 in a desired order and timing.
  • the assay device 10 can be configured in detail as follows.
  • Each of the plurality of assay modules 11 is configured as follows. As shown in FIG. 9, the assay module 11 has a microchannel 12 formed to allow the liquid L to flow.
  • the assay module 11 has a top 11a and a bottom 11b facing each other in the height direction of the microchannel 12.
  • Each of the top 11a and the bottom 11b can be made of a plastic film or sheet. However, the top and bottom are not limited to this.
  • the micro flow path 12 is defined in the height direction by the top portion 11a and the bottom portion 11b.
  • the flow direction, width direction, and height direction of the micro flow path 12 are substantially orthogonal to each other. Therefore, as shown in FIG. 8, it can be said that the plurality of assay modules 11 are arranged in the width direction of such a microchannel 12.
  • the microchannel 12 has one end side in the flow direction of the liquid L, that is, the end portion on the upstream side (indicated by the arrow F1 on one side) and the other end side in the flow direction of the fluid L, that is, Extends to and from the downstream end (indicated by one-sided arrow F2).
  • the liquid L can flow from the upstream side to the downstream side of the micro flow path 12 based on the lateral flow generated in the micro flow path 12.
  • the assay module 11 has an inlet 13 formed to allow the liquid L to flow into the microchannel 12. As shown in FIG. 9, the injection port 13 is arranged at the upstream end of the microchannel 12. The inlet 13 is formed so as to penetrate the top 11a.
  • the assay module 11 also has an absorbent porous medium 14 located at the downstream end of the microchannel 12. The absorbing porous medium 14 is configured to be able to absorb the liquid L in the microchannel 12.
  • the assay module 11 has an assay region 15 located in the middle of the flow direction of the microchannel 12.
  • a reagent that specifically binds to the sample in the assay is immobilized in the assay region 15.
  • Reagents involved in signal generation derived from specimens and reference substances include immobilization reagents that are used to pre-fix to the microchannel 12 and microcurrents in the assay process. There are additive reagents used to add to path 12.
  • the immobilization reagent provided in the assay region 15 specifically reacts with the sample in the liquid L and, together with the additive reagent, produces a detectable result of the sample.
  • Specimen detectable results can be observably visible to the naked eye, for example based on color changes, etc., or specimen detectable results can only be detected by a spectroscope or other measuring means. It can also appear.
  • the immobilization reagent provided in the assay region 15 is colored by reaction with an enzyme, an antibody, an epitope, a nucleic acid, a cell, an aptamer, a peptide, a molecular imprint polymer, an adsorption polymer, an adsorption gel, or a sample (III). It can be a chemical such as an ion, a color reagent, or any other substance that produces detectable results by reacting with a sample.
  • the immobilization reagent can be an antibody.
  • the immobilization reagent can be immobilized in the assay region 15 by a well-known immobilization technique such as a physical adsorption method or a chemisorption method.
  • Immobilization reagents include radioactive isotopes, enzymes, gold colloids, coloring reagents, quantum dots, colored molecules such as latex, dyes, electrochemical reactants, fluorescent substances, or luminescence to analyze or amplify the detection signal. Any labeling substance such as a substance can be bound. Alternatively, such labeling material can be attached to an additive reagent used to be added to the microchannel 12 in the assay step. Specifically, the immobilization reagent can be immobilized on one or both of the top 11a and the bottom 11b that define the microchannel 12 in its height direction.
  • the assay is performed in a state where the liquid L is fluidized in the microchannel 12 or the liquid L is allowed to stand in the microchannel 12 or is temporarily stopped. Will be done. Typically, the sample concentration in the liquid L can be detected.
  • the assay module 11 has a confirmation region 16 arranged to line up with the assay region 15 in the flow direction.
  • the confirmation region 16 is located downstream of the assay region 15.
  • the assay region 15 and the confirmatory region 16 are separated from each other to the extent that these generated signals are distinguishable and detectable.
  • the confirmation region 16 is configured to generate a known reaction (second reaction) that can be considered to have the same reaction time as the reaction (first reaction) that occurs in the assay region 15.
  • the confirmation region 16 is provided with an immobilization reagent that specifically binds to the reference substance.
  • the immobilization reagent of the confirmation region 16 can also be an antibody or the like, like the immobilization reagent of the assay region 15. Any labeling substance can be bound to this immobilization reagent.
  • This immobilization reagent can also be immobilized on one or both of the top 11a and the bottom 11b that define the microchannel 12 in its height direction.
  • the assay module 11 has an assay window 17 and a confirmation window 18 formed so that the assay region 15 and the confirmation region 16 can be confirmed from the outside, respectively.
  • the assay and confirmation windows 17, 18 are formed on the top 11a of the assay module 11. Each of the assay and confirmation windows 17, 18 is formed to penetrate the top of the assay module. However, at least one of the assay and confirmation windows can be transparent. At least one of the assay and confirmation windows can be translucent.
  • the liquid supply device 20 can be configured as follows in detail. As shown in FIG. 3, the discharge ports 21c of the plurality of containers 21 in the liquid supply device 20 are arranged in the dropping section. As shown in FIGS. 4 to 6B, in the liquid supply device 20, the plurality of containers 21 are detachably attached to the container attachment mechanism 22. Further, the plurality of containers 21 are removably held by the plurality of container holders 24 of the container mounting mechanism 22. The plurality of container holders 24 are also detachably attached to the container attachment mechanism 22 separately.
  • the liquid supply device 20 includes three containers 21 and three container holders 24 for holding them, respectively.
  • the liquid supply device is not limited to a configuration including three containers and three container holders.
  • the liquid feeder may include two containers and two container holders each holding them, or may include four or more containers and four or more container holders each holding them.
  • the capacity of each container 21 can be about 3 ml (milliliter) or more. More preferably, the capacity of each container 21 can be from about 5 ml to about 15 ml. However, the capacity of the container is not limited to these.
  • the plurality of containers 21 and the plurality of container holders 24 in the container mounting mechanism 22 and the plurality of movable members (also referred to as a plurality of push members) 23a in the container pushing mechanism 23 are along the movement path of the assay device 10. They are lined up. As will be described later, when the movement path of the assay device 10 is substantially along a straight line, a plurality of containers 21 and a plurality of container holders 24 in the container mounting mechanism 22 and a plurality of movable members 23a in the container pushing mechanism 23. Means substantially along a straight line. The plurality of container holders 24 are separated from each other.
  • the plurality of containers and the plurality of container holders in the container mounting mechanism and the plurality of movable members in the container pushing mechanism are, for example, a substantially arc line. It can be lined up along a line including a curve such as a wavy line. At least two of the plurality of container holders can be integrated.
  • each of the plurality of containers 21 can be configured as follows. As shown in FIGS. 5, 6A, and 6B, the main body 21a of the container 21 is formed in a substantially tubular shape extending along the container axis 21d passing through the discharge port 21c. Further, the main body portion 21a can be formed into a substantially cylindrical shape centered on the container axis 21d. However, the shape of the main body of the container is not limited to these. For example, the main body portion can be formed into a substantially elliptical cylinder shape, a substantially polygonal cylinder shape, or the like.
  • the main body portion 21a of the container 21 is located on the base end side of the container 21 in the direction along the container axis 21d.
  • the nozzle portion 21b of the container 21 is located on the tip end side of the container 21 in the direction along the container axis 21d.
  • the main body portion 21a and the nozzle portion 21b are detachably attached to each other.
  • the container 21 is arranged so that the discharge port 21c faces downward and the container axis 21d faces the vertical direction.
  • the container can be arranged so that the container axis is oriented in an inclined direction with respect to the vertical direction.
  • each of such a plurality of container holders 24 can be configured as follows. As shown in FIGS. 5, 6A, and 6B, the main body accommodating portion 24a of the container holder 24 has a receiving space 24e formed so as to correspond to the main body portion 21a of the container 21 accommodated therein. The receiving space 24e of the container holder 24 extends along the holder axis 24f. The passage port 24c of the container holder 24 penetrates the main body accommodating portion 24a in a direction intersecting the holder axis 24f, particularly in a direction substantially orthogonal to the holder axis 24f.
  • the main body accommodating portion 24a of the container holder 24 is located on the base end side of the container holder 24 in the direction along the holder axis 24f.
  • the nozzle holding portion 24b of the container holder 24 is located on the tip end side of the container holder 24 in the direction along the holder axis 24f.
  • the main body accommodating portion 24a and the nozzle holding portion 24b are detachably attached to each other.
  • the passage port 24c is located in the distal end side region of the main body accommodating portion 24a. As shown in FIGS.
  • the receiving portion 24d faces the passing port 24c in the receiving space 24e in the advancing / retreating direction (indicated by the arrows W on both sides) of the movable member 23a of the container pushing mechanism 23 described later. It is arranged like this.
  • the nozzle holding portion 24b of the container holder 24 has an insertion hole 24g through which the nozzle portion 21b of the container 21 can be inserted.
  • the nozzle holding portion 24b of the container holder 24 is formed so as to hold the nozzle 21b in a state where the nozzle portion 21b of the container 21 is inserted into the insertion hole 24g and the discharge port 21c of the container 21 is arranged outside the container holder 24. Will be done.
  • the container pushing mechanism 23 has a plurality of fixing members 23b that each support a plurality of movable members 23a.
  • the container pushing mechanism 23 includes a plurality of actuators 23c having a movable member 23a and a fixing member 23b.
  • the container pushing mechanism 23 has three movable members 23a configured to be able to push each of the three containers 21, and the container pushing mechanism 23 also has three movable members 23a.
  • Each includes three actuators 23c having three fixing members 23b and one movable member 23a and one fixing member 23b.
  • the container pushing mechanism can have two movable members and two fixing members, or can have four or more movable members and four or more fixing members each supporting them. Therefore, the container pushing mechanism can include two actuators or four or more actuators.
  • Each of the plurality of actuators 23c can be configured as follows. As shown in FIGS. 6A and 6B, the fixing member 23b is configured to allow the movable member 23a to be driven forward and backward.
  • the movable member 23a is movable between an advanced state (shown in FIG. 6A) advanced from the fixed member 23b and a retracted state (shown in FIG. 6B) retracted into the fixed member 23b.
  • the movable member 23a extends substantially linearly in the advancing / retreating direction.
  • the movable member 23a can be formed in a substantially rod shape. In this case, the width of the movable member 23a in the direction substantially orthogonal to the advancing / retreating direction can be about 3 mm or more and less than about 1 cm.
  • the movable member 23a can be formed into a substantially cylindrical shape.
  • the diameter of the movable member 23a can be about 3 mm or more and less than about 1 cm.
  • the shape of the movable member is not limited to this.
  • the mobile device 30 can be configured in detail as follows. As shown in FIGS. 1 and 2, the mobile device 30 has a stage 31 configured to detachably attach the assay device 10.
  • the moving device 30 has a guide assembly 32 that movably supports the stage 31.
  • the moving device 30 has a driving mechanism 33 that drives the stage 31 to move along the moving path.
  • the stage 31 is configured to support the bottom 10b of the assay device 10 from below with the top 10a of the assay device 10 facing upward.
  • the assay device 10 is arranged on the stage 31 so as to follow the movement path in the width direction thereof.
  • the guide assembly 32 has at least one guide portion 32a extending along the movement path of the assay device 10.
  • the guide assembly 32 has a base portion 32b that supports at least one guide portion 32a.
  • the drive mechanism 33 has a motor 33a.
  • the motor 33a has a drive shaft 33b that can be driven to rotate.
  • the drive mechanism 33 has a driven shaft 33c located distant from the drive shaft 33b on the opposite side of the drive shaft 33b in the direction along the movement path of the assay device 10.
  • the drive mechanism 33 has an endless belt 33d arranged along the movement path of the assay device 10.
  • the endless belt 33d is hung on the drive shaft 33b and the driven shaft 33c.
  • the stage 31 is attached to the endless belt 33d.
  • the rotary drive of the drive shaft 33b of the motor 33a drives the endless belt 33d in a loop shape, whereby the stage 31 moves along the moving path together with the endless belt 33d.
  • the drive mechanism of the mobile device is not limited to this.
  • such a mobile device 30 has a drop section (shown in FIG. 3) and a measurement section (shown in FIG. 10) so that the stage 31 can be equipped with the assay device 10. It can be moved to an installation section away from (shown).
  • the installation section is a section in which the stage 31 is in a state where the assay device 10 can be installed.
  • the dropping section is arranged between the installation section and the measurement section in the movement path of the assay device 10.
  • the moving device 30 can move the stage 31 together with the assay device 10 from the installation section to the measurement section via the dropping section along the moving path.
  • the moving device 30 can move the stage 31 together with the assay device 10 from the measurement section to the installation section via the dropping section.
  • the moving device 30 can repeatedly reciprocate the stage 31 together with the assay device 10 between the dropping section and the measuring section.
  • the stage 31 is configured to be able to move along the travel path with the assay device 10 to be able to receive.
  • the assay region 15 and the confirmation region 16 of each assay module 11 in the assay device 10 are imaged through the lens 61a of the image pickup unit 61 of the measurement device 60 described later.
  • the stage 31 is configured to be able to move along the movement path together with the assay device 10.
  • the liquid detection device 50 can be configured in detail as follows.
  • the liquid detection device 50 has a plurality of liquid detection units 51 configured to be capable of detecting each of the liquids L dropped from the discharge ports 21c of the plurality of containers 21.
  • Each of the plurality of liquid detection units 51 can be configured as follows. As shown in FIG. 7, the liquid detection unit 51 is located directly below the container 21. The liquid detection unit 51 is configured to be able to detect the liquid L dropped from the discharge port 21c of the container 21 in a non-contact manner.
  • the liquid detection unit 51 is a fiber sensor. However, the liquid detection unit is not limited to this.
  • the measuring device 60 can be configured in detail as follows.
  • the measuring device 60 includes electronic components such as a CPU (Central Processing Unit), a RAM (Random Access Memory), a ROM (Read Only Memory), a flash memory, an input interface, and an output interface, and an electric circuit in which such electronic components are arranged. Can be configured to include.
  • the measuring device 60 has an imaging unit 61 configured to be able to image the assay region 15 and the confirmation region 16 of the assay device 10.
  • the measuring device 60 has a concentration identification unit 62 that can identify the concentrations of the assay and confirmation regions 15 and 16 based on the image captured by the imaging unit 61.
  • the imaging unit 61 has a lens 61a and images the assay region 15 and the confirmation region 16 through the lens 61a.
  • the image pickup unit 61 can be a camera.
  • the lens 61a is arranged in the measurement section.
  • the lens 61a is arranged directly above the movement path of the assay region 15 and the confirmation region 16 in the plurality of assay modules 11 of the assay device 10.
  • the measuring device 60 can be a smartphone (mobile phone, cell phone) with a camera function.
  • the measuring device is not limited to this.
  • the measuring device may be a personal computer or the like to which a camera is connected.
  • the stage 31 of the moving device 30 is moved to the installation section.
  • the assay device 10 is installed on stage 31.
  • the stage 31 on which the assay device 10 is installed is moved from the installation section to the dropping section.
  • the injection port 13 of the first assay module 11 selected from the injection ports 13 of the plurality of assay modules 11 in the assay device 10 is selected from the discharge ports 21c of the plurality of containers 21 in the liquid supply device 20.
  • the stage 31 is moved so as to correspond to the discharge port 21c of the first container 21.
  • the first movable member 23a of the container pushing mechanism 23 corresponding to the first container 21 is advanced, whereby the first movable member 23a pushes the main body portion 21a of the first container 21.
  • the liquid L in the first container 21 is dropped from the discharge port 21c to the injection port 13 of the first assay module 11.
  • the first movable member 23a is retracted, whereby the first movable member 23a is retracted.
  • the first movable member 23a separates from the main body portion 21a of the first container 21.
  • the assay region 15 and the confirmation region 16 of the first assay module 11 are filled with the liquid L from the first container 21.
  • the injection port 13 of the second assay module 11 selected from the injection ports 13 of the plurality of assay modules 11 in the assay device 10 is the first container selected from the discharge ports 21c of the plurality of containers 21 in the liquid supply device 20. It is moved so as to correspond to the discharge port 21c of 21.
  • the first movable member 23a of the container pushing mechanism 23 corresponding to the first container 21 is advanced, whereby the first movable member 23a pushes the main body portion 21a of the first container 21.
  • the liquid L in the first container 21 is dropped from the discharge port 21c to the injection port 13 of the second assay module 11.
  • the first movable member 23a When the first liquid detection unit 51 of the liquid detection device 50 corresponding to the discharge port 21c of the first container 21 detects the dropped liquid L, the first movable member 23a is retracted, whereby the first movable member 23a is retracted.
  • the first movable member 23a separates from the main body portion 21a of the first container 21.
  • the assay region 15 and the confirmation region 16 of the second assay module 11 are filled with the liquid L from the first container 21.
  • the injection port 13 of the first assay module 11 selected from the injection ports 13 of the plurality of assay modules 11 in the assay device 10 is selected from the discharge ports 21c of the plurality of containers 21 in the liquid supply device 20. It is moved so as to correspond to the discharge port 21c of the container 21 of.
  • the second movable member 23a of the container pushing mechanism 23 corresponding to the second container 21 is advanced, whereby the second movable member 23a pushes the main body portion 21a of the second container 21.
  • the liquid L in the second container 21 is dropped from the discharge port 21c to the injection port 13 of the first assay module 11.
  • the second movable member 23a When the second liquid detection unit 51 of the liquid detection device 50 corresponding to the discharge port 21c of the second container 21 detects the dropped liquid L, the second movable member 23a is retracted, whereby the second movable member 23a is retracted.
  • the second movable member 23a separates from the main body portion 21a of the second container 21.
  • the assay region 15 and the confirmation region 16 of the first assay module 11 are filled with the liquid L from the second container 21.
  • the injection port 13 of the second assay module 11 selected from the injection ports 13 of the plurality of assay modules 11 in the assay device 10 is the second container selected from the discharge ports 21c of the plurality of containers 21 in the liquid supply device 20. It is moved so as to correspond to the discharge port 21c of 21.
  • the second movable member 23a of the container pushing mechanism 23 corresponding to the second container 21 is advanced, whereby the second movable member 23a pushes the main body portion 21a of the second container 21.
  • the liquid L in the second container 21 is dropped from the discharge port 21c to the injection port 13 of the second assay module 11.
  • the second movable member 23a When the second liquid detection unit 51 of the liquid detection device 50 corresponding to the discharge port 21c of the second container 21 detects the dropped liquid L, the second movable member 23a is retracted, whereby the second movable member 23a is retracted.
  • the second movable member 23a separates from the main body portion 21a of the second container 21.
  • the assay region 15 and the confirmation region 16 of the second assay module 11 are filled with the liquid L from the second container 21.
  • the stage 31 on which the assay device 10 is installed is moved from the dropping section to the measuring section.
  • the assay and confirmation regions 15 and 16 of the first assay module 11 are moved so as to correspond to the lens 61a of the imaging unit 61 of the measuring device 60.
  • the assay unit 61 captures the assay and confirmation regions 15 and 16 of the first assay module 11.
  • the measuring device 60 identifies the concentration in the assay and confirmation regions 15 and 16 of the first assay module 11 based on the captured image.
  • the assay and confirmation regions 15 and 16 of the second assay module 11 are moved so as to correspond to the lens 61a of the imaging unit 61 of the measuring device 60.
  • the assay unit 61 captures the assay and confirmation regions 15 and 16 of the second assay module 11.
  • the measuring device 60 identifies the concentration in the assay and confirmation regions 15 and 16 of the second assay module 11 based on the captured image.
  • the outline of the assay system is an assay system including an assay device 10 configured to perform an assay using a liquid L, so that the liquid L can be dropped onto the assay device 10.
  • the liquid supply device 20 configured in the above, the moving device 30 configured to make the assay device 10 relatively movable in the horizontal direction with respect to the liquid supply device 20, and the liquid by the liquid supply device 20.
  • a plurality of liquid supply devices 20 are configured in the form of an instillation container, including a control device 40 configured to be able to control the dropping of L and the movement of the assay device 10 by the moving device 30.
  • Each container 21 includes a main body portion 21a formed so as to be able to accommodate the liquid L, and a discharge port configured to be capable of dropping the liquid L contained in the main body portion 21a.
  • the liquid supply device 20 includes a nozzle portion 21b having a 21c, and the liquid supply device 20 can attach the plurality of containers 21 in a state where the discharge ports 21c of the plurality of containers 21 are oriented downward and are spaced apart from each other in the horizontal direction. At least one configured to be able to push the main body portion 21a of the plurality of containers 21 in order to drip the liquid L from the container mounting mechanism 22 configured to do so and the discharge ports 21c of the plurality of containers 21.
  • the control device 40 includes a container pushing mechanism 23 having a movable member 23a, in order for the at least one movable member 23a to push the main body portions 21a of the plurality of containers 21 in a desired order and timing. It is configured to control the container pushing mechanism 23.
  • the liquid L is supplied from the discharge port 21c of the container 21 and the main body 21a of the plurality of containers 21 is pushed by at least one movable member 23a. Since the timing can be easily controlled, the timing of supplying the plurality of liquids L can be accurately controlled.
  • the configuration for supplying the liquid L by using a plurality of containers 21 configured in the form of an eye drop container and a container pushing mechanism 23 having at least one movable member 23a for pushing the main body portion 21a of the plurality of containers 21 is simple. be.
  • the operation of putting the liquid L into the container 21 configured in the form of an eye drop container is easy, and since the plurality of liquid Ls are separately housed in the plurality of containers 21, the risk of contamination can be reduced. Therefore, a plurality of liquids L can be supplied quickly and continuously, a small amount of liquid L can be supplied with high accuracy, a configuration for supplying the liquid L can be simplified, and contamination can occur. The risk can be reduced.
  • the container mounting mechanism 22 has a plurality of container holders 24 for holding the plurality of containers 21, and each container holder 24 holds the container 21.
  • the main body housing portion 24a for receiving the main body portion 21a of the container holder 24 and the nozzle holding portion 24b for holding the nozzle portion 21b of the container 21 held by the container holder 24 are provided. It has a passage port 24c that opens so as to allow passage of the movable member 23a that pushes the main body portion 21a of the container 21 housed therein.
  • a plurality of containers 21 can be easily attached to and detached from the container mounting mechanism 22. Therefore, for example, if a plurality of containers 21 each containing a plurality of reagents prepared so as to be different from each other are prepared in advance, a container 21 containing a desired reagent can be attached to the container mounting mechanism from these plurality of containers 21. If installed at 22, the risk of contamination between reagents can be prevented.
  • the container 21 in which the liquid L remains after use can be removed from the container mounting mechanism 22, and the removed container 21 can be stored in a refrigerator or the like, and then the stored container 21 is used again.
  • the container 21 can be attached to the container mounting mechanism 22 again and used. Therefore, the consumption of the liquid L such as the reagent can be reduced, and the container 21 can be used repeatedly.
  • the main body accommodating portion 24a of the container holder 24 is pushed by the movable member 23a to push the main body portion 21a of the container 21. Therefore, a small amount of liquid L can be supplied with high accuracy from the discharge port 21c of the container 21.
  • the main body accommodating portion 24a of each container holder 24 is formed so as to receive the main body portion 21a of the container 21 pushed by the movable member 23a passing through the passage port 24c. It has a receiving portion 24d.
  • the main body accommodating portion 24a of the container holder 24 can stably support the main body portion 21a of the container 21 pushed by the movable member 23a, it is possible to stably support the main body portion 21a of the container 21 from the discharge port 21c of such a container 21.
  • a small amount of liquid L can be supplied with high accuracy.
  • the outline of the assay system includes a liquid detection device 50 configured to be able to separately detect the liquid L dropped from the discharge port 21c of the nozzle portion 21b in the plurality of containers 21, and the control device 40.
  • the liquid detection device 50 detects the liquid L dripping from the discharge port 21c of each container 21, it changes from the advanced state in which the main body 21a of the container 21 is pushed to the retracted state in which the main body 21a is not pushed. It is configured to control the container pushing mechanism 23 in order to return it.
  • the timing of supplying the liquid L from the discharge port 21c of the container 21 can be accurately controlled.
  • the outline of the assay system includes a measuring device 60 configured to be capable of measuring the reaction obtained by the assay device 10 by the liquid L dropped from the liquid supply device 20, and the measuring device 60 is provided.
  • the control device 40 is arranged so as to be horizontally separated from the liquid supply device 20, and the measuring device 60 drops the liquid L from the liquid supply device 20 to the assay device 10.
  • the assay device 10 is horizontally relative to the liquid supply device 20 and the measurement device 60 toward a measurement section in which the reaction obtained by the assay device 10 is measured by the liquid L dropped in the section. It is configured to control the moving device 30 so as to move to.
  • the measuring device 60 measures the reaction obtained by the liquid L in the assay device 10. be able to. Therefore, the timing of supplying the plurality of liquids L can be accurately controlled so that the measuring device 60 appropriately measures the reaction of the assay device 10.
  • the assay device 10 has a plurality of assay modules 11 configured so that a reaction can be obtained by a liquid L dropped from the liquid supply device 20.
  • the supply device 20 is configured to allow the liquid L to be dropped onto the plurality of assay modules 11 in the desired order and timing, and the measuring device 60 is said by the liquid L dropped from the liquid supply device 20.
  • the control device 40 is configured so that the reactions obtained by the plurality of assay modules 11 can be measured in a desired order and timing, and the control device 40 measures the reactions of the plurality of assay modules 11 by the measuring device 60.
  • the liquid supply device 20 and the movement of the liquid supply device 20 so that the timing is the same with respect to the order and timing of dropping the liquid L from the liquid supply device 20 to the plurality of assay modules 11 at regular time intervals. It is configured to control the device 30 and the measuring device 60.
  • the measuring device 60 determines the time required for these assays.
  • the reaction of these assay modules 11 can be appropriately measured in the above order and timing.
  • the timing of supplying the plurality of liquids L can be accurately controlled so that the measuring device 60 appropriately measures the reaction of the assay device 10.
  • the reaction times of the plurality of assay modules 11 can be made uniform.
  • the container pushing mechanism 23 has a plurality of movable members 23a arranged so as to be able to push the main body portions 21a of the plurality of containers 21 respectively.
  • the moving device 30 is configured to be able to move the assay device 10 along a predetermined movement path, and the plurality of containers 21 make the discharge port 21c of these nozzle portions 21b the assay device 10.
  • the control device 40 arranges the container pushing mechanism 23 so that the plurality of movable members 23a push the plurality of containers 21 in the desired order and timing, respectively. It is configured to be controlled.
  • the body portion 21a of the plurality of containers 21 can be pushed more quickly and continuously by the plurality of movable members 23a in a desired order and timing, and as a result, the plurality of containers 21 can be pushed.
  • the liquid L can be supplied more quickly and continuously from the discharge port 21c of the above.
  • FIGS. 11 to 13 An outline of the assay system according to the present embodiment will be described with reference to FIGS. 11 to 13. As shown in FIGS. 11 to 13, the assay system according to the present embodiment is generally configured in the same manner as the assay system according to the first embodiment.
  • the assay system according to the present embodiment is schematically and in detail the same as the assay device 10, the mobile device 30, and the measuring device 60 according to the first embodiment. It has 10, a moving device 30, and a measuring device 60.
  • the assay system according to the present embodiment has a liquid supply device 120 corresponding to the liquid supply device 20 according to the first embodiment.
  • the assay system according to the present embodiment has a control device 140 and a liquid detection device 150 corresponding to the control device 40 and the liquid detection device 50 according to the first embodiment, respectively.
  • the liquid supply device 120 has a plurality of containers 21 according to the first embodiment and a plurality of containers 21 substantially and in detail similarly configured.
  • the liquid supply device 120 has a container mounting mechanism 122 and a container pushing mechanism 123 corresponding to the container mounting mechanism 22 and the container pushing mechanism 23 according to the first embodiment, respectively.
  • the container mounting mechanism 122 has a container holder 124 corresponding to a plurality of container holders 24 according to the first embodiment.
  • the container pushing mechanism 123 has a movable member 123a corresponding to the movable member 23a according to the first embodiment.
  • Each container holder 124 has a main body accommodating portion 24a, a nozzle holding portion 24b, a passing port 24c, and a main body accommodating portion 124a corresponding to a receiving portion 24d, a nozzle holding portion 124b, a passing port 124c, and a receiving portion, respectively, according to the first embodiment. It has a portion 124d.
  • the liquid supply device 120 of the assay system has a container moving mechanism 125 configured to allow a plurality of containers 21 to be moved with respect to the container pushing mechanism 123.
  • the container pushing mechanism 123 has one movable member 123a configured to be able to push a plurality of containers 21 separately.
  • the control device 140 is electrically connected to the liquid supply device 120, the mobile device 30, the liquid detection device 150, and the measuring device 60.
  • the control device 140 is configured to control the container moving mechanism 125 so that the plurality of containers 21 move to the container pushing position corresponding to one movable member 123a in a desired order and timing.
  • the control device 140 is also configured to control the container pushing mechanism 123 so that one movable member 123a pushes the main body 21a of the plurality of containers 21 moved by the container moving mechanism 125 in a desired order and timing. ing.
  • the liquid supply device 120 can be configured as follows in detail. As shown in FIG. 11, the discharge ports 21c of the plurality of containers 21 in the liquid supply device 120 are sequentially arranged one by one in the dropping section. As shown in FIGS. 11 and 13, in the liquid supply device 120, the plurality of containers 21 are detachably attached to the container attachment mechanism 122. Further, the plurality of containers 21 are removably held by the plurality of container holders 124 of the container mounting mechanism 122. The plurality of container holders 124 are integrally formed so as to form a holder assembly 126.
  • the liquid supply device 120 includes four containers 21 and four container holders 124 for holding them, respectively.
  • the liquid feeder may include two containers and two container holders each holding them, three containers and three container holders each holding them, or five or more. It may also include a container and five or more container holders holding each of them.
  • the plurality of containers 21 and the plurality of container holders 124 in the container mounting mechanism 122 are arranged along a substantially arc-shaped holder alignment line 126a.
  • the holder alignment line can have a shape other than the substantially arc shape.
  • the holder alignment line may have a substantially straight line shape or the like.
  • the plurality of container holders 124 are spaced apart from each other in the direction along the holder alignment line 126a (hereinafter referred to as "holder alignment direction").
  • the holder alignment line 126a is also arranged so as to pass through the dropping section.
  • One pushing member 123a in the container pushing mechanism 123 is arranged in the dropping area.
  • Each of such a plurality of container holders 124 can be configured as follows. As shown in FIG. 13, the main body accommodating portion 124a of the container holder 124 has a receiving space 124e formed so as to correspond to the main body portion 21a of the container 21 accommodated therein. The receiving space 124e of the container holder 124 extends along the holder axis 124f. The passage port 124c of the container holder 124 penetrates the main body accommodating portion 124a in a direction intersecting the holder axis 124f, particularly in a direction substantially orthogonal to the holder axis 124f.
  • the main body accommodating portion 124a of the container holder 124 is located on the base end side of the container holder 124 in the direction along the holder axis 124f.
  • the nozzle holding portion 124b of the container holder 124 is located on the tip end side of the container holder 124 in the direction along the holder axis 124f.
  • the main body accommodating portion 124a and the nozzle holding portion 124b are detachably attached to each other.
  • the main body accommodating portion 124a is opened so as to open the receiving space 124e to the outside of the holder 124 on the base end side of the holder 124.
  • the container 21 is accommodated in the receiving space 124e from the base end side of the opening main body accommodating portion 124a.
  • the passage port 124c also opens on the base end side of the main body accommodating portion 124a.
  • the receiving portion 124d is arranged in the receiving space 124e so as to face the passing port 124c in the advancing / retreating direction (indicated by the arrows W on both sides) of the movable member 123a of the container pushing mechanism 123.
  • the nozzle holding portion 124b of the container holder 124 has an insertion hole 124g penetrating so that the nozzle portion 21b of the container 21 can be inserted.
  • the nozzle holding portion 124b of the container holder 124 is formed so as to hold the nozzle 21b in a state where the nozzle portion 21b of the container 21 is inserted into the insertion hole 124g and the discharge port 21c of the container 21 is arranged outside the container holder 124. Will be done.
  • the holder assembly 126 is formed so as to have an outer peripheral edge portion 126b extending substantially following the holder alignment line 126a.
  • the holder assembly 126 can be formed so as to have a substantially arcuate outer peripheral edge portion 126b extending following the substantially arcuate holder alignment line 126a.
  • the holder assembly 126 can be formed so as to have a substantially circular outer peripheral edge portion 126b extending following the substantially circular holder alignment line 126a.
  • the holder assembly 126 is rotatably supported around the central axis 126c located at the substantially center of the substantially circular holder alignment line 126a, as indicated by the arrows Q on both sides.
  • the holder assembly 126 has a driven gear 126d having a plurality of teeth arranged substantially parallel to the holder alignment line 126a.
  • the passage port 124c of each container holder 124 penetrates the main body accommodating portion 124a of the container holder 124 from the receiving space 124e of the container holder 124 toward the outer peripheral edge portion 126b of the holder assembly 126.
  • the container moving mechanism 125 has a motor 125a configured to be rotatable and driveable. As shown by the arrows P on both sides, the container moving mechanism 125 has a drive gear 125b configured to be rotatable about the central axis 125c by rotationally driving the motor 125a.
  • the drive gear 125b has a plurality of teeth arranged along its outer circumference. A plurality of teeth of the drive gear 125b mesh with the driven gear 126d.
  • the drive gear 125b is rotated by the rotational drive of the motor 125a, whereby the driven gear 126d that meshes with the drive gear 125b is moved.
  • the holder assembly 126 is operated so that the plurality of container holders 124 move along the holder alignment line 126a.
  • the plurality of containers 21 can be moved to the container pushing positions corresponding to one movable member 123a in a desired order and timing.
  • the container pushing mechanism 123 has one fixing member 123b that supports one movable member 123a.
  • the container pushing mechanism 123 includes one actuator 123c having one movable member 123a and one fixing member 123b. More specifically, the movable member 123a, the fixing member 123b, and the actuator 123c of the container pushing mechanism 123 are configured in the same manner as the movable member 23a, the fixing member 23b, and the actuator 23c according to the first embodiment.
  • the movable member 123a can move forward and backward as shown by the arrows W on both sides.
  • the liquid detection device 150 can be configured in detail as follows.
  • the liquid detection device 150 has one liquid detection unit 151 configured to be able to detect the liquid L dropped from the discharge port 21c of one container 21 located in the dropping section. More specifically, the liquid detection unit 151 is configured in the same manner as the liquid detection unit 51 according to the first embodiment.
  • stage 31 of the moving device 30 is moved to the installation section.
  • the assay device 10 is installed on stage 31.
  • the container moving mechanism 125 moves the first container 21 selected from the plurality of containers 21 in the liquid supply device 120 so as to correspond to one movable member 123a in the container pushing mechanism 123 in the dropping section.
  • the stage 31 on which the assay device 10 is installed is moved from the installation section to the dropping section.
  • the injection port 13 of the first assay module 11 selected from the injection ports 13 of the plurality of assay modules 11 in the assay device 10 corresponds to the discharge port 21c of the first container 21. Move the stage 31.
  • the movable member 123a is advanced, whereby the movable member 123a pushes the main body portion 21a of the first container 21.
  • the liquid L in the first container 21 is dropped from the discharge port 21c to the injection port 13 of the first assay module 11.
  • the movable member 123a is retracted, whereby the movable member 123a is separated from the main body portion 21a of the first container 21.
  • the concentrations in the assay region 15 and the confirmation region 16 of the first assay module 11 change.
  • the container moving mechanism 125 moves the second container 21 selected from the plurality of containers 21 in the liquid supply device 120 so as to correspond to one movable member 123a in the container pushing mechanism 123 in the dropping section.
  • the injection port 13 of the second assay module 11 selected from the injection ports 13 of the plurality of assay modules 11 in the assay device 10 corresponds to the discharge port 21c of the second container 21. Move the stage 31.
  • the movable member 123a is advanced, whereby the movable member 123a pushes the main body portion 21a of the second container 21.
  • the liquid L in the second container 21 is dropped from the discharge port 21c to the injection port 13 of the second assay module 11.
  • the movable member 123a is retracted, whereby the movable member 123a is separated from the main body portion 21a of the second container 21.
  • the concentrations in the assay region 15 and the confirmation region 16 of the second assay module 11 change.
  • the stage 31 on which the assay device 10 is installed is moved from the dropping section to the measuring section.
  • the assay and confirmation regions 15 and 16 of the first assay module 11 are moved so as to correspond to the lens 61a of the imaging unit 61 of the measuring device 60.
  • the assay unit 61 captures the assay and confirmation regions 15 and 16 of the first assay module 11.
  • the measuring device 60 identifies the concentration in the assay and confirmation regions 15 and 16 of the first assay module 11 based on the captured image.
  • the assay and confirmation regions 15 and 16 of the second assay module 11 are moved so as to correspond to the lens 61a of the imaging unit 61 of the measuring device 60.
  • the assay unit 61 captures the assay and confirmation regions 15 and 16 of the second assay module 11.
  • the measuring device 60 identifies the concentration in the assay and confirmation regions 15 and 16 of the second assay module 11 based on the captured image.
  • the liquid supply device 120 includes a container moving mechanism 125 configured to make the plurality of containers 21 movable with respect to the container pushing mechanism 123.
  • the container pushing mechanism 123 has one movable member 123a configured so that the main body portions 21a of the plurality of containers 21 can be pushed separately, and the control device 140 has the plurality of.
  • the container 21 is configured to control the container moving mechanism 125 in order to move to the container pushing position corresponding to the one movable member 123a in the desired order and timing, and the one movable member 123a.

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Abstract

Le problème décrit par la présente invention est de fournir un système de dosage par lequel une pluralité de liquides sont fournis rapidement et consécutivement, une minuscule quantité de liquide est fournie avec une précision élevée, la configuration de l'alimentation en liquide est simplifiée, la manipulation du liquide est simplifiée et le risque de contamination est réduit. La solution selon la présente invention concerne un système de dosage comprenant un dispositif de dosage (10) qui effectue un dosage au moyen d'un liquide (L). Ce système de dosage comporte : un dispositif d'alimentation en liquide (20, 120) qui fait s'égoutter un liquide sur un dispositif de dosage ; un dispositif de déplacement (30) qui déplace le dispositif de dosage ; et un dispositif de commande (40, 140) qui commande le dispositif d'alimentation en liquide et le dispositif mobile. Le dispositif d'alimentation en liquide comprend une pluralité de récipients (21) formés respectivement sous la forme d'un récipient d'instillation, et présente au moins un élément mobile (23a, 123a) qui pousse des corps principaux des multiples récipients de telle sorte qu'un liquide s'égoutte à partir d'orifices d'évacuation (21c) des multiples récipients. Le dispositif de commande commande l'élément ou les éléments mobiles de façon à pousser les corps principaux de la pluralité de récipients dans un ordre souhaité et à un moment souhaité.
PCT/JP2021/032467 2020-09-07 2021-09-03 Système de dosage WO2022050384A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022546985A JPWO2022050384A1 (fr) 2020-09-07 2021-09-03

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JP2020-149810 2020-09-07
JP2020149810 2020-09-07

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WO2022050384A1 true WO2022050384A1 (fr) 2022-03-10

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4130224A (en) * 1976-10-08 1978-12-19 Envair, Inc. Viscous liquid dispenser
US4515294A (en) * 1982-03-31 1985-05-07 Southern Chemical Products Company Liquid dispenser, valve therefor and process of producing the valve
JPS6393373A (ja) * 1986-10-09 1988-04-23 Showa Denko Kk 液滴化装置
JPH0562253U (ja) * 1991-06-04 1993-08-20 株式会社アルメックス 点滴速度監視装置
JPH0737051A (ja) * 1993-07-22 1995-02-07 Brother Ind Ltd 溶液の更新時期告知装置
US6092695A (en) * 1992-05-11 2000-07-25 Cytologix Corporation Interchangeable liquid dispensing cartridge pump
US20150048119A1 (en) * 2012-05-08 2015-02-19 Roche Diagnostics Operations, Inc. Microfluidic dispenser, cartridge and analysis system for analyzing a biological sample
WO2019045118A1 (fr) * 2017-09-04 2019-03-07 国立研究開発法人産業技術総合研究所 Contenant d'emballage de liquide et dispositif d'éjection de liquide

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4130224A (en) * 1976-10-08 1978-12-19 Envair, Inc. Viscous liquid dispenser
US4515294A (en) * 1982-03-31 1985-05-07 Southern Chemical Products Company Liquid dispenser, valve therefor and process of producing the valve
JPS6393373A (ja) * 1986-10-09 1988-04-23 Showa Denko Kk 液滴化装置
JPH0562253U (ja) * 1991-06-04 1993-08-20 株式会社アルメックス 点滴速度監視装置
US6092695A (en) * 1992-05-11 2000-07-25 Cytologix Corporation Interchangeable liquid dispensing cartridge pump
JPH0737051A (ja) * 1993-07-22 1995-02-07 Brother Ind Ltd 溶液の更新時期告知装置
US20150048119A1 (en) * 2012-05-08 2015-02-19 Roche Diagnostics Operations, Inc. Microfluidic dispenser, cartridge and analysis system for analyzing a biological sample
WO2019045118A1 (fr) * 2017-09-04 2019-03-07 国立研究開発法人産業技術総合研究所 Contenant d'emballage de liquide et dispositif d'éjection de liquide

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