WO2021201791A1

WO2021201791A1 - Propolis extract containing lactic acid

Info

Publication number: WO2021201791A1
Application number: PCT/TR2020/050448
Authority: WO
Inventors: Şadan KAYHAN
Original assignee: Api̇park Aricilik Üreti̇m Danişmanlik Eği̇ti̇m Islah Ve Arge İthalat İhracat Sanayi̇ Ti̇caret A.Ş.
Priority date: 2020-04-03
Filing date: 2020-05-22
Publication date: 2021-10-07

Abstract

A propolis extract developed to be used in fields of food, cosmetics and health characterized in that containing lactic acid syrup as solvent, and water to dilute propolis and lactic acid syrup. Said lactic acid syrup contains lactic acid ranging 20% to 85% by weight, and propolis extract contains 30-99% by weight of lactic acid syrup, 1-60% by weight of water and 5-30% by weight of water.

Description

PROPOLIS EXTRACT CONTAINING LACTIC ACID

Technical Field

The invention relates to propolis extract which contains lactic acid, developed to be used at food, cosmetics and health industries, and its production method thereof.

In particular, the invention relates to a propolis extract has area of usage in food industry as supplement and conservative agent, in cosmetics and healthy industries, which is obtained by propolis solubilized in a mixture of lactic acid and water and production method thereof.

State of Art

Nowadays, dietary habits tend to change along with changing lifestyle and working conditions. People apply to supplement foods for many reasons such as supplementing normal dietary, to feel more energized, to enhance their immune system by taking vitamins and minerals which are not sufficiently present in the food substances.

In the state of art propolis which included in the supplementary food additives, is a resin-like product produced by bees processing the collected materials, which are from coniferous trees especially from buds, leaves, trunks and excretions, with the enzymes secreted from secretory glands located in their heads. People used propolis as a medicine throughout the history, due to its rich ingredients. Different from cerago, propolis contains vitamins B1 , B2, C, E, and also is rich in minerals such as zinc, ferrum, calcium, magnesium, sodium and potassium. It is known to be used to treat various diseases due to its antioxidant, antiseptic, antibacterial, antiviral, antifungal, anticancer and anti-inflammatory effects. Propolis, known to eliminate harmful viruses and bacteria as used by people, is a natural antibiotic which can be used both by adults and children to enhance their immune system. However, unprocessed propolis is in a mixture-state with a material similar to beeswax.

Ethanol, propylene glycol, glycerin, water and various oils usually used as solvents for propolis extracts. For instance, TR 2012/08415 discloses an herbal treatment with propolis. According to such disclosure, composition of an extract containing ethanol, used for medical purposes, pure and natural bee propolis, pure cerago and water components with particular mixture rates. Nevertheless, ethanol (ethyl alcohol) is primarily not halal and also propolis extracts containing ethanol are proved to be harmful for human body in some studies. Water cannot sufficiently solve propolis as a solvent.

CN105560289A discloses a liquid propolis preparation technique. Disclosed technology consists of following steps: tearing raw propolis material down, preparation of liquid mixture consists of caprylic/ capric triglyceride, propylene glycol, glycerin, pure water and some alcohol, mixing two components together, filling and packaging liquid propolis mixture.

As known from above identified patents and many more studies propylene glycol, an alternative solvent, dissolves propolis very well but may cause serious side-effects. Adequate amount of such solvent may cause high acidity in blood where emergency medical attention required. Acidosis development, which may cause rapid coma and kidney failure, was observed with the patients who were injected with psychiatric medicines containing propylene glycol. At the same time, propylene glycol toxicity may cause symptoms similar to sepsis or serious inflammatory response syndrome.

In other aspects, there is a possibility to observance of some side-effects with propolis dissolved with glycerin. Since glycerin is not fully absorbed by body, consuming glycerin in great amounts alone or in combination with other food substances may cause gas and diarrhea. Also, if herbal glycerin is ingested may cause dizziness, headache, nausea, vomiting and excessive thirst.

Besides, extraction process gets slower if various oils are used as solvents and pre treatment of propolis and various temperature applications are needed.

TR 2019/20656 discloses water based liquid propolis contains boron-based components prepared according to human dietary and liquidized with a special technology. Such composition may cause various side-effects on health due to boron composition.

As conclusion, a novelty is required in related technical field due to above mentioned disadvantages and requirement of extracts with propolis without any side-effects on health and prolonging shelf-life on which product they might be used in. Purpose of the Invention

Purpose of the present invention is to develop propolis extract containing lactic acid to be used in food, cosmetics and health industries and their production thereof.

The specific purpose of the invention is to develop a propolis extract having no side-effect to human body and therefore, having no maximum in-take limit which achieved by fully metabolizing aqueous lactic acid solution naturally in body.

Another purpose of the invention is to enable dissolution of propolis in lactic acid solution without even crumbling since solution rate of propolis in lactic acid solution is very high. However, in the current technic lactic acid needs to be pulverized in order to be solved in solvents such as ethanol, propylene alcohol, glycerin and oil.

Another purpose of the invention is to reveal more of strong antioxidant, antiseptic, antibacterial, antiviral, antifungal, anticancer and anti-inflammatory characteristics of propolis compared against present art. Lactic acid solution in propolis extraction observed to have 3 times more phenolic value than the ethanol solution in propolis extraction. Phenolic compounds known to have antioxidant effect.

A purpose of the invention is to develop a propolis extract which can be consumed by every religious group since lactic acid solution is halal.

Another purpose of the invention is to develop a propolis extract can be consumed by vegetarians who do not consume meat, milk and dairy products and people who are allergic to dairy products or lactose intolerant.

Another purpose of the invention is to develop a propolis extract which can be used in functional foods such as soda, yoghurt, ice cream (for example soda with propolis, yoghurt with propolis, ice cream with propolis etc.)

One of the purposes of the invention is to develop a propolis extract suitable to be used in fields of skin care and cosmetics.

Another purpose of the invention is to develop a propolis extract which can present shelf life extending effects due to antimicrobial, antifungal effects of the propolis and lactic acid acting as conservative. Therefore, if subject matter propolis extract is added to food substances, in amount ranging 0,1% to 2%, provides extension in shelf life of such food substances naturally and creates an alternative to chemical conservatives.

A propolis extract containing propolis and lactic acid solution as dissolver and their production method thereof is developed in scope of the invention in order to accomplish above disclosed purposes.

Method developed in scope of the invention in order to realize above disclosed purposes consists of following steps;

Mixing lactic acid and water at room temperature and obtaining lactic acid solution,

Mixing propolis into lactic acid solution in small pieces,

Keeping propolis, lactic acid solution and water mixture in a dark room and in an acid-resistant material at temperature range O-dOΌ for 1 day to 4 weeks,

Stirring such mixture during duration of hold,

Filtration of mixture consisting of liquid and solid particles by the end of the hold duration and obtaining liquid and pure propolis extract.

In a preferred embodiment of the invention, dilution of lactic acid solution contains water.

In order to accomplish above identified purposes, said lactic acid solution contains lactic acid ranging 20% to 85% by weight.

In order to accomplish above identified purposes, said propolis extract contains 30- 99% by weight of lactic acid solution, 1 - 60% by weight of propolis and 5- 30% by weight of water.

In a preferred embodiment of the invention, said propolis, lactic acid solution and water mixture is kept in glass bottles in a dark room for 2 weeks at 21 Ό room temperature.

In a preferred embodiment of the invention, the obtained mixture is stirred for 5 minutes daily during the holding time.

In a preferred embodiment of the invention, the mixture containing liquid and solid particles is filtrated 1 to 7 times at the end of the duration of hold.

In a preferred embodiment of the invention, obtained propolis extract can be consumed with dairy or liquid foodstuff by dripping in 5 to 10 drops daily. In a preferred embodiment of the invention, obtained propolis extract has conservative characteristic and can be added to food substances in amount ranging 0,1% to 2%.

Detailed Description of the Invention

The invention relates to propolis extract which developed to be used in fields of food, cosmetics and health and their production method thereof. Said propolis extract is obtained by dissolving propolis in lactic acid and water mixture.

Propolis which included in the extract of the subject invention is defined as “Natural antibiotics” and has inhibitor effect on 21 bacteria, 9 fungi, 3 protozoa and many virus species. As known to be used in treatment of various diseases due to its strong antioxidant, antiseptic, antibacterial, antiviral, antifungal, anticancer and anti-inflammatory effects. Clinical studies proof propolis is effective on respiratory diseases (bronchitis, COPD, influenza), skin diseases (herpes, dermatophyte, allergies, burn, skin ulcer, apse), periodontal diseases (gum disease, mouth sore), ear-nose-throat disorders, digestive system diseases (parasites, colitis, gastric ulcer, reflux), gyneacological diseases (vaginal and cervical diseases) and urinary system diseases (urinary tract infections). In recent years, many researches have been studied on the effect of propolis on cancer cells. Since propolis is effective against various cancer cells, it is suggested that propolis is effective against cancer independent from cancer type. Research, based on such powerful effect of the propolis, conducted to determine whether propolis has cytotoxic or apoptotic effect on cell line of acute lymphoblastic leukemia, and it was determined that caffeic acid and caffeic acid phenyl ester (CAPE) shows great cytotoxic effect and stops the growth of tumor cell. Food supplements such as propolis which are rich by polyphenolic compounds play an essential role on prevention and / or treatment of prostate cancer. It is expressed that propolis extract obtained from the plants having high antioxidant characteristic in our country may have antiproliferative effect on PC-3 prostate cancer. Patients are advised to use propolis during radiotherapy on cancer treatment. Propolis creates kind of a protective barrier over healthy cells and therefore prevents them to be effected by radioactive rays, and only cancerous, distorted cells are affected by such rays. Thus, intake of radiation levels is reduced. Also, due to its immune system enhancing properties, propolis supports vital functions of cancer patients and enhances body immunity of cancer patients. The potential positive results of propolis are seen in many geriatric and neurological diseases, considering that polyphenols are gaining increasing attention every day due to their role in reducing oxidative stress and protecting nerve cells, which are effective on aging, and that propolis is one of the best polyphenol-containing sources (especially flavonoids and phenolic acids). It has been determined that using propolis as diluted mouthwash greatly prevents many negativities such as mouth sores, bad breath, dental cavities, and toothpastes made of propolis give more effective results with fighting against cavities than the toothpastes made based of zinc and calcium hydroxide. Due to its high prophylactic effect, propolis is utmost successful in the treatment of cold irritation, hemorrhoid, first- and second-degree burns, calluses, boils, eczema, psoriasis, fungi, shingles and scars in the time of recovery during post-operative period. As known, propolis has protective and healing effects against peptic ulcer, an important digestive system disease. It is determined that propolis has utmost antibacterial effect against microbial diseases that occur in the respiratory system (Streptococcus pneumoniae, Haemophilus influenzae, Haemophilus parainfluenza, Moraxella catarrhalis and Streptococcus pyogenes, Mycobacterium humanus). Therefore, it is very effective against respiratory diseases such as flu, pneumonia, bronchitis, tuberculosis. Condensed propolis extract lowers the blood pressure, balances blood pressure and has a calming effect. Propolis is a healing source with its protective and complementary medical treatment properties against various diseases; since nowadays many people tend to have more natural diet.

On the other hand, another compound of the present invention lactic acid is monomer of polylactic acid and they are different from each other. While polylactic acid is a thermoplastic polyester also is a natural acid produced by bacteria in the lactic acid fermented food, and therefore daily intake amount is not limited. Polylactic acid is used to obtain solid products, however lactic acid solutions are liquid. Lactic acid acts chemically both as acid and as alcohol, thus propolis solubility is rapid and very strong when compared against other solvents. It has no side-effects and can be consumed by every religious group. Also, it is suitable for people who are allergic to dairy or lactose intolerant.

It is informed that a person who weighs 70 kilograms may safely consume daily 100 grams lactic acid.

Conclusively, water is used to dilute lactic acid syrup. Lactic acid- water mixture that contains 20% to 85% lactic acid by weight is used to dissolve propolis.

Table 1 : Content (in kg) and percentage of propolis extract subject to invention

Production Method:

Production method subject to invention comprises following process steps:

Mixing lactic acid and water according to desired percentage at room temperature under the open atmosphere and obtaining lactic acid solution (80%: 20% lactic acid: water mixture),

Propolis added in to the obtained lactic acid solution in small pieces and does not need to be pulverized since propolis dissolved good in lactic acid,

Keeping propolis, lactic acid solution and water mixture in a dark room and in any acid-resistant material, preferably in glass bottles, between temperature range 0- dOΌ, preferably at room temperature at 21 Ό for 1 day to 4 weeks, preferably for 2 weeks,

Stirring obtained mixture for 5 minutes daily during the duration of hold,

Filtration of the mixture consists of liquid and solid particles 1 to 7 times and obtaining the liquid and pure propolis extract and bottling of such extract.

Since the lactic acid solution dissolves the propolis quite well, continuous shaking is not required. Extraction temperature can be applied in temperature range 0 to 50^eC for said method.

The subject of the invention propolis extract should be consumed by dropping preferably into dairy products or any liquid foodstuff (water, ayran, lemonade, carbonated drinks) without dropping into mouth alone. Such product can be consumed 5 to 10 drops daily. Said propolis extract provides naturally extended shelf life to food substances which was added in the range of 0,1% to 2% and acts as a conservative agent, and stands out as an alternate product against chemical conservatives.

Also, such product can be used in skin care and peeling application in cosmetics field, can be also dropped and rubbed onto desired area after concentrated with water as medical treatment complementary and for its protective effective against various diseases in health industry.

Claims

1. The propolis extract developed to be used in fields of food, cosmetics and health characterized in that containing lactic acid syrup for solvent and propolis.

2. The extract as claimed in claim 1 , characterized in that said lactic acid contains water for dilution purposes.

3. The extract as claimed in claim 1 , characterized in that said lactic acid solution contains lactic acid ranging 20% to 85% by weight.

4. The extract as claimed in one of the claims 1 to 3, characterized in that said propolis extract consists of 30% to 99% by weight of lactic acid solution, 1% to 60% by weight of propolis and 5% to 30% by weight of water.

5. The extract as claimed in one of the claims 1 to 4, characterized in that can be consumed by dropping 5 to 10 drops daily into dairy products or liquid foodstuff.

6. The extract as claimed in one of the claims 1 to 5, characterized in that can be added to food substances in amount ranging 0,1% to 2% as conservative agent.

7. Production method of propolis extract to be used in the field of food, cosmetics and health characterized in that comprising the following steps;

Mixing propolis into lactic acid solution in small pieces,

Stirring such mixture during duration of hold,

8. A method in accordance to claim 7, characterized in that adding water for dilution of said lactic acid solution.

9. A method in accordance to claim 7, characterized in that said lactic acid solution contains lactic acid ranging 20% to 85% by weight.

10. A method in accordance to one of the claims 7 to 9, characterized in that said propolis extract contains 30- 99% by weight of lactic acid solution, 1-60% by weight of propolis and 5-30% by weight of water.

11. A method in accordance to claim 7, characterized in that lactic acid solution and water mixture is kept at room temperature at 21 Ό f or 2 weeks in a dark room and in glass bottles.

12. A method in accordance to claim 7, characterized in that the obtained mixture is stirred for 5 minutes daily during the duration of hold.

13. A method in accordance to claim 7, characterized in that the mixture containing liquid and solid particles is filtrated 1 to 7 times at the end of the duration of hold.

14. A method in accordance to one of the claims 7 to 13, characterized in that said propolis extract can be consumed by dropping 5 to 10 drops daily into dairy products or any liquid foodstuff.

15. A method in accordance to one of the claims 7 to 14, characterized in that said propolis extract can be added to food substances in amount ranging 0,1 to 2% as conservative agent.