WO2021167330A1 - 수축 제어가 가능한 진피층 개발, 및 이를 이용한 균일한 성능의 인공피부의 제조 - Google Patents
수축 제어가 가능한 진피층 개발, 및 이를 이용한 균일한 성능의 인공피부의 제조 Download PDFInfo
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- WO2021167330A1 WO2021167330A1 PCT/KR2021/001993 KR2021001993W WO2021167330A1 WO 2021167330 A1 WO2021167330 A1 WO 2021167330A1 KR 2021001993 W KR2021001993 W KR 2021001993W WO 2021167330 A1 WO2021167330 A1 WO 2021167330A1
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- WIPO (PCT)
- Prior art keywords
- biogel
- alginate
- gelatin
- skin
- cells
- Prior art date
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Abstract
Description
구분 | 시험결과 (단위: mm) | |||||
1 | 2 | 3 | 4 | 5 | 평균 | |
콜라겐 1 (세포 없음) |
9.62 | 9.58 | 9.55 | 9.51 | 9.54 | 9.56 |
콜라겐 2 (세포 있음, 7일 후) |
3.08 | 3.02 | 3.00 | 3.01 | 3.04 | 3.03 |
알지네이트 1(세포 없음) | 9.15 | 9.48 | 9.57 | 9.58 | 9.49 | 9.45 |
알지네이트 2(세포 있음, 7일 후) | 9.08 | 9.27 | 9.42 | 9.41 | 9.48 | 9.33 |
알지네이트 3(세포 있음, 14일 후) | 9.55 | 9.51 | 9.51 | 9.60 | 9.61 | 9.56 |
알지네이트 4(세포 있음, 21일 후) | 9.68 | 9.53 | 9.69 | 9.57 | 9.42 | 9.58 |
시료 | 1 | 2 | 3 | 평균 | 표준편차 |
W0 (g) | 0.5 | 0.5 | 0.5 | ||
W1 (g) | 9.868 | 9.333 | 9.420 | ||
W2 (g) | 9.875 | 9.351 | 9.440 | ||
초기 지지체 면적 (m2) | 0.002 | 0.002 | 0.002 | ||
흡수력 (g/m2/hr) | 8.849 | 17.699 | 17.699 | 13.274 | 6.194 |
수분 투과성 (%) | 4.755 | 4.902 | 4.838 | 4.83 | 0.073 |
Claims (9)
- 알지네이트 및 젤라틴을 포함하는 바이오겔, 및 상기 바이오겔에 혼입된 진피 형성세포를 포함하는 진피층; 및표피 형성세포를 포함하는 표피층을 포함하는 인공피부.
- 청구항 1에 있어서, 상기 바이오겔은 0.1 내지 10 중량%의 알지네이트, 및 5 내지 10 중량%의 젤라틴을 포함하는 것인 인공피부.
- 청구항 1에 있어서, 상기 진피 형성세포는 무세포 진피 파우더, 섬유아세포 또는 이들의 조합인 것인 인공피부.
- 청구항 1에 있어서, 상기 표피 형성세포는 각질형성세포인 것인 인공피부.
- 청구항 1에 있어서, 상기 진피층은 엘라스틴(Elastin), 키토산(Chitosan), 글리코사미노글루칸(GAGs; glycosaminoglycans), 히알루론산(HA; hyaluronic acid) 또는 이들의 조합인 진피를 구성하는 세포외기질 (extracellular matrix)을 더 포함하는 것인 인공피부.
- 청구항 1에 있어서, 상기 바이오겔에 피부 상태 개선용 또는 피부 질환 치료용 약물이 담지되는 것인 인공피부.
- 알지네이트 및 젤라틴을 포함하는 바이오겔을 겔화하는 단계;상기 겔화가 완료된 바이오겔에 진피 형성세포를 혼입하여 배양하는 단계; 및상기 배양을 통해 제조된 진피층에 표피 형성세포를 부착시켜 배양하는 단계를 포함하는 인공피부를 제조하는 방법.
- 청구항 7에 있어서, 상기 바이오겔을 겔화하는 단계는 캐스팅 겔(casting gel)을 1차 겔화하는 단계; 및 겔화된 캐스팅 겔 상에서 알지네이트 및 젤라틴을 포함하는 바이오 겔을 2차 겔화하는 단계를 포함하는 것인 방법.
- 청구항 7 또는 8에 있어서, 상기 바이오겔은 0.1 내지 10 중량%의 알지네이트, 및 5 내지 10 중량%의 젤라틴을 포함하는 것인 방법.
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