WO2021128320A1 - Dispositif de culture de souche microbienne - Google Patents

Dispositif de culture de souche microbienne Download PDF

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Publication number
WO2021128320A1
WO2021128320A1 PCT/CN2019/129383 CN2019129383W WO2021128320A1 WO 2021128320 A1 WO2021128320 A1 WO 2021128320A1 CN 2019129383 W CN2019129383 W CN 2019129383W WO 2021128320 A1 WO2021128320 A1 WO 2021128320A1
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WO
WIPO (PCT)
Prior art keywords
culture chamber
culture
pipe
incubator
chamber body
Prior art date
Application number
PCT/CN2019/129383
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English (en)
Chinese (zh)
Inventor
蒋伯康
Original Assignee
蒋伯康
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 蒋伯康 filed Critical 蒋伯康
Priority to PCT/CN2019/129383 priority Critical patent/WO2021128320A1/fr
Publication of WO2021128320A1 publication Critical patent/WO2021128320A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • A01G18/60—Cultivation rooms; Equipment therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Definitions

  • the invention relates to the technical field of microbial strains, in particular to a microbial strain cultivation equipment.
  • Microbial strains are divided into feed fermentation strains and biological fertilizer fermentation strains.
  • This product can not only make up for the amino acids that are easily lacking in conventional feed, but also can quickly transform the nutrient components of other coarse feed materials to achieve the effect of enhancing digestion, absorption and utilization.
  • the fermented feed uses microorganisms and complex enzymes as biological feed starter strains, and Feed raw materials are transformed into microbial cell protein, biologically active small peptide amino acids, microbial active probiotics, and compound enzyme preparations into a whole biological fermented feed.
  • the product can not only make up for the amino acids that are easily lacking in conventional feeds, but also can quickly transform the nutrient components of other rough feed raw materials to achieve the effect of enhancing digestion, absorption and utilization.
  • the existing microbial strains need to be cultivated, but the existing microbial strains cultivation equipment can no longer meet the needs of the existing market.
  • the invention provides a microbial strain culture equipment, which is safe and convenient to use. By setting the dual protection of an incubator and a culture room, the microbial strains can be better protected, and the microbial strains are prevented from being affected by different strains during the cultivation process.
  • these strains will cause the microbial strains to die during the cultivation process, resulting in the reduction of the survival rate of the microbial strains, and the problem of affecting the cultivation efficiency of the incubator; the temperature inside the incubator can be monitored at all times to prevent people from being unable to timely Know the frequency of changes in the internal temperature of the incubator, leading to the large-scale death of microbial species; monitor the growth of microbial species in real time through the high-definition camera, and collect pictures to ensure the normal growth of microbial species, which can make the temperature more uniform and make the culture The precision is higher, and the cultivated ones can better meet the daily requirements, and can effectively solve the problems in the background art mentioned above.
  • a microbial strain culture equipment including a culture chamber body, a cooling pipe, a sprayer, a first temperature and humidity sensor, a thermal pad, a regulator, a water inlet pipe, a water pump, a control box, PLC controller, timer, second temperature and humidity sensor, blower, blade, rotating shaft, cooling and heating regulator, support rod, water inlet, high-definition camera, camera sealing protective cover, mobile incubator, fixed incubator, protective cover, The second incubation room, the first incubation room, the base, the temperature and temperature controller, the tray, the fixed nut, the rotating motor, the heating tube, the support rod, the culture dish, the greenhouse, the preheating box, the exhaust fan, the air inlet pipe, the air outlet Tube, heating tube and air pipe, the top of the culture chamber body is provided with a protective cover, the inner side of the culture chamber body is provided with a first culture chamber, and the bottom end of the first culture chamber is provided with a mobile
  • the bottom of the chamber body is provided with a thermal insulation pad
  • the other side of the inside of the culture chamber body is provided with a second culture chamber
  • the inside of the second culture chamber is provided with a fixed incubator
  • the bottom end of the fixed incubator is provided with
  • the base the upper right of the base is provided with a temperature controller
  • the left side of the temperature controller is provided with a tray
  • the rotating electric machine is installed under the tray
  • fixing nuts are arranged on both sides of the lower side of the rotating electric machine, so A heating tube is arranged below the rotating electric machine.
  • a waterproof fog film is provided on the outer side of the high-definition camera.
  • the rotating shaft is connected with each other by bolts.
  • the air outlet of the exhaust fan is connected with a dust-proof net through a movable buckle.
  • the top view of the tray is circular.
  • a heat dissipation strip is provided on the outside of the control box.
  • a partition is provided between the second culturing chamber and the first culturing chamber, and the partition is connected to the body of the culturing chamber by bolts.
  • the present invention has the following beneficial effects: it is safe and convenient to use.
  • the microbial strains can be better protected, and the microbial strains are prevented from being affected by different bacteria during the cultivation process.
  • the interference of species, these species will cause the death of the microbial species during the cultivation process, resulting in the reduction of the survival rate of the microbial species, and the problem of affecting the cultivation efficiency of the incubator; the temperature value inside the incubator can be monitored at all times to avoid people Unable to know the change frequency of the internal temperature of the incubator in time, leading to the large-scale death of microbial species; monitor the growth of microbial species in real time through the high-definition camera, and collect pictures to ensure the normal growth of microbial species, which can make the temperature more uniform and make The training accuracy is higher, and the training can better meet the daily requirements.
  • Figure 1 is a schematic diagram of the structure of the present invention
  • Figure 2 is a schematic diagram of the structure of the fixed incubator of the present invention.
  • Figure 3 is a schematic diagram of the structure of the mobile incubator of the present invention.
  • Figure 4 is a schematic diagram of the structure of the regulator of the present invention.
  • FIG. 5 is a schematic diagram of the installation structure of the cooling pipe of the present invention.
  • Temperature Temperature controller 28, tray; 29, fixed nut; 30, rotating motor; 31, heating tube; 32, support rod; 33, petri dish; 34, greenhouse; 35, preheating box; 36, exhaust fan; 37 3. Air inlet pipe; 38. Air outlet pipe; 39. Heating pipe; 40. Air pipe.
  • the present invention provides a microbial strain culture equipment, including a culture chamber body 1, a cooling pipe 2, a sprayer 3, a first temperature and humidity sensor 4, an insulation pad 5, and a regulator 6.
  • the top of the culture chamber body 1 is provided with a protective cover 23, the inner side of the culture chamber body 1 is provided with a first culture chamber 25, and the bottom end of the first culture chamber 25 is provided with a mobile incubator 21.
  • the interior of the mobile incubator 21 is provided.
  • a support rod 32 There is a support rod 32, a petri dish 33 is placed on one side of the support rod 32, a preheating box 35 is installed at the bottom of the adjusting chamber 34, an exhaust fan 36 is installed outside the upper end of the preheating box 35, and the upper end outside of the exhaust fan 36 is connected with
  • the inlet pipe 37, the exhaust fan 36 is connected with an outlet pipe 38 outside one end, a heating pipe 39 is installed inside the preheating box 35, one end of the preheating box 35 is connected to one end of the air pipe 40, and the other end of the air pipe 40
  • the top of the cultivation room body 1 is provided with a high-definition camera 19, the outside of the high-definition camera 19 is provided with a camera sealing protection cover 20, a cooling pipe 2 is provided under the high-definition camera 19, and one side of the cooling pipe 2 is provided
  • a sprayer 3 one end of the cooling pipe 2 is provided with a water inlet 18, the middle of
  • a control box 9 is provided on one side of the control box 9, a PLC controller 10 is provided inside the control box 9, a rotating shaft 15 is provided in the middle of the blower 13, a blade 14 is provided on one side of the rotating shaft 15, and one side of the blower 13 is provided
  • the heat and cold regulator 16, the first temperature and humidity sensor 4 is installed on the inner wall of the left side of the culture chamber body 1, the inner wall of the right side of the culture chamber body 1 is provided with a second temperature sensor 12, and the bottom of the culture chamber body 1 is provided with an insulation pad 5 ,
  • the other side of the culture chamber body 1 is provided with a second culture chamber 24, the inside of the second culture chamber 24 is provided with a fixed incubator 22, the bottom end of the fixed incubator 22 is provided with a base 26, and the upper right of the base 26 is provided
  • There is a temperature controller 27, the left side of the temperature controller 27 is provided with a tray 28, the rotating electric machine 30 is installed under the tray 28, the lower two sides of the rotating electric machine 30 are provided with fixing nuts 29, and the rotating electric machine
  • a waterproof fog film is provided on the outer side of the high-definition camera 19.
  • the rotating shafts 15 and 14 are connected by bolts.
  • the air outlet of the exhaust fan 36 is connected with a dust-proof net through a movable buckle.
  • the top view of the tray 28 is circular.
  • a heat dissipation strip is provided on the outside of the control box 9.
  • a partition is provided between the second culture chamber 24 and the first culture chamber 25, and the partition and the culture chamber body 1 are connected by bolts.
  • the advantages of the present invention are that it is safe and convenient to use.
  • the microbial strains can be better protected, and the microbial strains are prevented from being interfered by different strains during the cultivation process.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif de culture de souche microbienne, comprenant un corps de chambre de culture (1), un incubateur mobile (21), un incubateur fixe (22), un couvercle de protection (23), une seconde chambre de culture (24), et une première chambre de culture (25), le couvercle de protection (23) étant disposé au niveau de l'extrémité supérieure du corps de chambre de culture (1), la première chambre de culture (25) étant située sur un côté de l'intérieur du corps de chambre de culture (1), et l'incubateur mobile (21) étant situé au niveau de l'extrémité inférieure de la première chambre de culture (25). L'utilisation est sûre et pratique, et les souches microbiennes peuvent être mieux protégées au moyen de la double protection des incubateurs et des chambres de culture ; la température à l'intérieur des incubateurs peut être surveillée à tout moment, ce qui permet d'éviter le problème de la mort à grande échelle de souches microbiennes en raison de l'incapacité de connaître la fréquence des changements au niveau de la température interne des incubateurs d'une manière opportune ; la croissance des souches microbiennes peut être surveillée en temps réel au moyen d'une caméra haute définition, des images étant recueillies pour assurer la croissance normale des souches microbiennes ; et la température peut être rendue plus uniforme de sorte que la précision de la culture soit plus élevée et que la culture puisse mieux répondre aux besoins quotidiens.
PCT/CN2019/129383 2019-12-27 2019-12-27 Dispositif de culture de souche microbienne WO2021128320A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/129383 WO2021128320A1 (fr) 2019-12-27 2019-12-27 Dispositif de culture de souche microbienne

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/129383 WO2021128320A1 (fr) 2019-12-27 2019-12-27 Dispositif de culture de souche microbienne

Publications (1)

Publication Number Publication Date
WO2021128320A1 true WO2021128320A1 (fr) 2021-07-01

Family

ID=76575415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2019/129383 WO2021128320A1 (fr) 2019-12-27 2019-12-27 Dispositif de culture de souche microbienne

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WO (1) WO2021128320A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN203072509U (zh) * 2012-12-19 2013-07-24 李丹丹 教学用恒温培养箱
US20170022468A1 (en) * 2015-04-23 2017-01-26 Comecer S.P.A. Modular Incubator
CN206580817U (zh) * 2017-03-22 2017-10-24 广州九妆化妆品有限公司 一种高精度生化培养箱
CN206978245U (zh) * 2017-04-08 2018-02-09 江苏丰收菇业有限公司 一种食用蘑菇智能培养室
CN207002729U (zh) * 2017-05-09 2018-02-13 辽宁精研药物评价试验平台有限公司 一种多功能恒温恒湿培养箱
CN207468647U (zh) * 2017-08-24 2018-06-08 苏州科创生物技术有限公司 一种对比隔离式二氧化碳培养箱

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN203072509U (zh) * 2012-12-19 2013-07-24 李丹丹 教学用恒温培养箱
US20170022468A1 (en) * 2015-04-23 2017-01-26 Comecer S.P.A. Modular Incubator
CN206580817U (zh) * 2017-03-22 2017-10-24 广州九妆化妆品有限公司 一种高精度生化培养箱
CN206978245U (zh) * 2017-04-08 2018-02-09 江苏丰收菇业有限公司 一种食用蘑菇智能培养室
CN207002729U (zh) * 2017-05-09 2018-02-13 辽宁精研药物评价试验平台有限公司 一种多功能恒温恒湿培养箱
CN207468647U (zh) * 2017-08-24 2018-06-08 苏州科创生物技术有限公司 一种对比隔离式二氧化碳培养箱

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