WO2021128320A1

WO2021128320A1 - Microbial strain culture device

Info

Publication number: WO2021128320A1
Application number: PCT/CN2019/129383
Authority: WO
Inventors: 蒋伯康
Original assignee: 蒋伯康
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2021-07-01

Abstract

A microbial strain culture device, comprising a culture chamber body (1), a moving incubator (21), a fixed incubator (22), a protective cover (23), a second culture chamber (24), and a first culture chamber (25), the protective cover (23) being arranged at the top end of the culture chamber body (1), the first culture chamber (25) being arranged on one side of the interior of the culture chamber body (1), and the moving incubator (21) being arranged at the bottom end of the first culture chamber (25). Use is safe and convenient, and the microbial strains can be better protected by means of the dual protection of the incubators and the culture chambers; the temperature inside the incubators can be monitored at any time, avoiding the problem of the large-scale death of microbial strains due to being unable to know the frequency of changes to the internal temperature of the incubators in a timely manner; the growth of the microbial strains can be monitored in real time by means of a high-definition camera, pictures being collected to ensure the normal growth of the microbial strains; and the temperature can be made more uniform such that the precision of the culturing is higher and the culturing can better meet daily needs.

Description

Microbial strain cultivation equipment

Technical field

The invention relates to the technical field of microbial strains, in particular to a microbial strain cultivation equipment.

Background technique

Microbial strains are divided into feed fermentation strains and biological fertilizer fermentation strains. This product can not only make up for the amino acids that are easily lacking in conventional feed, but also can quickly transform the nutrient components of other coarse feed materials to achieve the effect of enhancing digestion, absorption and utilization. The fermented feed uses microorganisms and complex enzymes as biological feed starter strains, and Feed raw materials are transformed into microbial cell protein, biologically active small peptide amino acids, microbial active probiotics, and compound enzyme preparations into a whole biological fermented feed. The product can not only make up for the amino acids that are easily lacking in conventional feeds, but also can quickly transform the nutrient components of other rough feed raw materials to achieve the effect of enhancing digestion, absorption and utilization. However, the existing microbial strains need to be cultivated, but the existing microbial strains cultivation equipment can no longer meet the needs of the existing market.

Therefore, how to design a microbial strain culture equipment has become a problem we have to solve at present.

Summary of the invention

The invention provides a microbial strain culture equipment, which is safe and convenient to use. By setting the dual protection of an incubator and a culture room, the microbial strains can be better protected, and the microbial strains are prevented from being affected by different strains during the cultivation process. Interference, these strains will cause the microbial strains to die during the cultivation process, resulting in the reduction of the survival rate of the microbial strains, and the problem of affecting the cultivation efficiency of the incubator; the temperature inside the incubator can be monitored at all times to prevent people from being unable to timely Know the frequency of changes in the internal temperature of the incubator, leading to the large-scale death of microbial species; monitor the growth of microbial species in real time through the high-definition camera, and collect pictures to ensure the normal growth of microbial species, which can make the temperature more uniform and make the culture The precision is higher, and the cultivated ones can better meet the daily requirements, and can effectively solve the problems in the background art mentioned above.

In order to achieve the above objective, the present invention provides the following technical solutions: a microbial strain culture equipment, including a culture chamber body, a cooling pipe, a sprayer, a first temperature and humidity sensor, a thermal pad, a regulator, a water inlet pipe, a water pump, a control box, PLC controller, timer, second temperature and humidity sensor, blower, blade, rotating shaft, cooling and heating regulator, support rod, water inlet, high-definition camera, camera sealing protective cover, mobile incubator, fixed incubator, protective cover, The second incubation room, the first incubation room, the base, the temperature and temperature controller, the tray, the fixed nut, the rotating motor, the heating tube, the support rod, the culture dish, the greenhouse, the preheating box, the exhaust fan, the air inlet pipe, the air outlet Tube, heating tube and air pipe, the top of the culture chamber body is provided with a protective cover, the inner side of the culture chamber body is provided with a first culture chamber, and the bottom end of the first culture chamber is provided with a mobile incubator A support rod is arranged inside the mobile incubator, a petri dish is placed on one side of the support rod, a preheating box is installed at the bottom of the adjusting chamber, and an exhaust fan is installed outside the upper end of the preheating box, An air inlet pipe is connected to the outside of the upper end of the exhaust fan, an outlet pipe is connected to the outside of one end of the exhaust fan, a heating pipe is installed inside the preheating box, and an air pipe is connected to one side of the preheating box At one end, the other end of the air pipe extends to the inside of the culture chamber, the top of the culture chamber body is provided with a high-definition camera, the outside of the high-definition camera is provided with a camera sealing protective cover, and a cooling pipe is provided under the high-definition camera A sprayer is arranged on one side of the cooling pipe, a water inlet is arranged on one end of the cooling pipe, a support rod is arranged in the middle of the cooling pipe, the water inlet pipe is installed on one side of the culturing chamber body, and the inlet One end of the water pipe is provided with a water pump, one side of the water pump is provided with a control box, the inside of the control box is provided with a PLC controller, the middle of the blower is provided with a rotating shaft, and one side of the rotating shaft is provided with a blade A cooling and heating regulator is provided on one side of the blower, the first temperature and humidity sensor is installed on the inner wall of the left side of the culture chamber body, and the inner wall of the right side of the culture chamber body is provided with a second temperature sensor. The bottom of the chamber body is provided with a thermal insulation pad, the other side of the inside of the culture chamber body is provided with a second culture chamber, the inside of the second culture chamber is provided with a fixed incubator, and the bottom end of the fixed incubator is provided with The base, the upper right of the base is provided with a temperature controller, the left side of the temperature controller is provided with a tray, the rotating electric machine is installed under the tray, and fixing nuts are arranged on both sides of the lower side of the rotating electric machine, so A heating tube is arranged below the rotating electric machine.

According to the above technical solution, a waterproof fog film is provided on the outer side of the high-definition camera.

According to the above technical solution, the rotating shaft is connected with each other by bolts.

According to the above technical solution, the air outlet of the exhaust fan is connected with a dust-proof net through a movable buckle.

According to the above technical solution, the top view of the tray is circular.

According to the above technical solution, a heat dissipation strip is provided on the outside of the control box.

According to the above technical solution, a partition is provided between the second culturing chamber and the first culturing chamber, and the partition is connected to the body of the culturing chamber by bolts.

Compared with the prior art, the present invention has the following beneficial effects: it is safe and convenient to use. By setting the dual protection of the incubator and the culture room, the microbial strains can be better protected, and the microbial strains are prevented from being affected by different bacteria during the cultivation process. The interference of species, these species will cause the death of the microbial species during the cultivation process, resulting in the reduction of the survival rate of the microbial species, and the problem of affecting the cultivation efficiency of the incubator; the temperature value inside the incubator can be monitored at all times to avoid people Unable to know the change frequency of the internal temperature of the incubator in time, leading to the large-scale death of microbial species; monitor the growth of microbial species in real time through the high-definition camera, and collect pictures to ensure the normal growth of microbial species, which can make the temperature more uniform and make The training accuracy is higher, and the training can better meet the daily requirements.

Description of the drawings

The accompanying drawings are used to provide a further understanding of the present invention, and the description constitutes a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention.

In the attached picture:

Figure 1 is a schematic diagram of the structure of the present invention;

Figure 2 is a schematic diagram of the structure of the fixed incubator of the present invention;

Figure 3 is a schematic diagram of the structure of the mobile incubator of the present invention;

Figure 4 is a schematic diagram of the structure of the regulator of the present invention;

5 is a schematic diagram of the installation structure of the cooling pipe of the present invention;

Labels in the figure: 1. Culture room body; 2. Cooling pipe; 3. Sprayer; 4. First temperature and humidity sensor; 5. Insulation pad; 6. Regulator; 7. Water inlet pipe; 8. Water pump; 9. Control box 10, PLC controller; 11, timer; 12, second temperature and humidity sensor; 13, blower; 14, blades; 15, rotating shaft; 16, heat and cold regulator; 17, support rod; 18, water inlet; 19. HD camera; 20. Camera sealed protective cover; 21. Mobile incubator; 22. Fixed incubator; 23. Protective cover; 24. Second incubation room; 25. First incubation room; 26. Base; 27. Temperature Temperature controller; 28, tray; 29, fixed nut; 30, rotating motor; 31, heating tube; 32, support rod; 33, petri dish; 34, greenhouse; 35, preheating box; 36, exhaust fan; 37 3. Air inlet pipe; 38. Air outlet pipe; 39. Heating pipe; 40. Air pipe.

Detailed ways

The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not used to limit the present invention.

Example: As shown in Figures 1-5, the present invention provides a microbial strain culture equipment, including a culture chamber body 1, a cooling pipe 2, a sprayer 3, a first temperature and humidity sensor 4, an insulation pad 5, and a regulator 6. Water inlet pipe 7, water pump 8, control box 9, PLC controller 10, timer 11, second temperature and humidity sensor 12, blower 13, blade 14, rotating shaft 15, cooling and heating regulator 16, support rod 17, water inlet 18 , HD camera 19, camera sealing protective cover 20, mobile incubator 21, fixed incubator 22, protective cover 23, second incubation chamber 24, first incubation chamber 25, base 26, temperature controller 27, tray 28, fixed Nut 29, rotating motor 30, heating pipe 31, support rod 32, petri dish 33, greenhouse 34, preheating box 35, exhaust fan 36, air inlet pipe 37, air outlet pipe 38, heating pipe 39 and air pipe 40, The top of the culture chamber body 1 is provided with a protective cover 23, the inner side of the culture chamber body 1 is provided with a first culture chamber 25, and the bottom end of the first culture chamber 25 is provided with a mobile incubator 21. The interior of the mobile incubator 21 is provided. There is a support rod 32, a petri dish 33 is placed on one side of the support rod 32, a preheating box 35 is installed at the bottom of the adjusting chamber 34, an exhaust fan 36 is installed outside the upper end of the preheating box 35, and the upper end outside of the exhaust fan 36 is connected with The inlet pipe 37, the exhaust fan 36 is connected with an outlet pipe 38 outside one end, a heating pipe 39 is installed inside the preheating box 35, one end of the preheating box 35 is connected to one end of the air pipe 40, and the other end of the air pipe 40 Extending to the inside of the cultivation room 8, the top of the cultivation room body 1 is provided with a high-definition camera 19, the outside of the high-definition camera 19 is provided with a camera sealing protection cover 20, a cooling pipe 2 is provided under the high-definition camera 19, and one side of the cooling pipe 2 is provided There is a sprayer 3, one end of the cooling pipe 2 is provided with a water inlet 18, the middle of the cooling pipe 2 is provided with a support rod 17, and the water inlet pipe 7 is installed on one side of the cultivation chamber body 1, and one end of the water inlet pipe 7 is provided with a water pump 8. A control box 9 is provided on one side of the control box 9, a PLC controller 10 is provided inside the control box 9, a rotating shaft 15 is provided in the middle of the blower 13, a blade 14 is provided on one side of the rotating shaft 15, and one side of the blower 13 is provided The heat and cold regulator 16, the first temperature and humidity sensor 4 is installed on the inner wall of the left side of the culture chamber body 1, the inner wall of the right side of the culture chamber body 1 is provided with a second temperature sensor 12, and the bottom of the culture chamber body 1 is provided with an insulation pad 5 , The other side of the culture chamber body 1 is provided with a second culture chamber 24, the inside of the second culture chamber 24 is provided with a fixed incubator 22, the bottom end of the fixed incubator 22 is provided with a base 26, and the upper right of the base 26 is provided There is a temperature controller 27, the left side of the temperature controller 27 is provided with a tray 28, the rotating electric machine 30 is installed under the tray 28, the lower two sides of the rotating electric machine 30 are provided with fixing nuts 29, and the rotating electric machine 30 is provided with a heating tube 31.

According to the above technical solution, a waterproof fog film is provided on the outer side of the high-definition camera 19.

According to the above technical solution, the rotating

shafts

15 and 14 are connected by bolts.

According to the above technical solution, the air outlet of the exhaust fan 36 is connected with a dust-proof net through a movable buckle.

According to the above technical solution, the top view of the tray 28 is circular.

According to the above technical solution, a heat dissipation strip is provided on the outside of the control box 9.

According to the above technical solution, a partition is provided between the second culture chamber 24 and the first culture chamber 25, and the partition and the culture chamber body 1 are connected by bolts.

Based on the above, the advantages of the present invention are that it is safe and convenient to use. By setting the dual protection of the incubator and the culture room, the microbial strains can be better protected, and the microbial strains are prevented from being interfered by different strains during the cultivation process. These strains will cause the microbial strains to die during the cultivation process, resulting in the reduction of the survival rate of the microbial strains, and the problem of affecting the cultivation efficiency of the incubator; the temperature inside the incubator can be monitored at all times to prevent people from being unable to know in time The frequency of changes in the internal temperature of the incubator leads to the death of a large area of microbial strains; real-time monitoring of the growth of microbial strains through high-definition cameras, and collecting pictures to ensure the normal growth of microbial strains can make the temperature more uniform and make the culture more accurate. High, the cultivated ones can better meet the daily requirements.

Finally, it should be noted that the above are only preferred examples of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, it is still possible for those skilled in the art. The technical solutions described in the foregoing embodiments are modified, or some of the technical features are equivalently replaced. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

A microbial strain culture equipment, including a culture chamber body (1), a cooling pipe (2), a sprayer (3), a first temperature and humidity sensor (4), an insulation pad (5), a regulator (6), and a water inlet pipe (7), water pump (8), control box (9), PLC controller (10), timer (11), second temperature and humidity sensor (12), blower (13), blade (14), rotating shaft ( 15) Heat and cold regulator (16), support rod (17), water inlet (18), high-definition camera (19), camera sealing protection cover (20), mobile incubator (21), fixed incubator (22) , Protective cover (23), second culture chamber (24), first culture chamber (25), base (26), temperature controller (27), tray (28), fixing nut (29), rotating motor ( 30), heating pipe (31), support rod (32), petri dish (33), greenhouse (34), preheating box (35), exhaust fan (36), air inlet pipe (37), air outlet pipe (38) A heating tube (39) and a gas pipe (40), characterized in that: the top of the culture chamber body (1) is provided with a protective cover (23), and one side of the inside of the culture chamber body (1) A first culture chamber (25) is provided, a mobile incubator (21) is provided at the bottom end of the first culture chamber (25), and a support rod (32) is provided inside the mobile incubator (21), so A petri dish (33) is placed on one side of the support rod (32), a preheating box (35) is installed at the bottom of the conditioning chamber (34), and an exhaust fan is installed outside the upper end of the preheating box (35) (36), an air inlet pipe (37) is connected to the outer side of the upper end of the exhaust fan (36), an air outlet pipe (38) is connected to the outer side of one end of the exhaust fan (36), and the preheating box (35) A heating pipe (39) is installed inside the preheating box (35), one end of the air pipe (40) is connected to one side of the preheating box (35), and the other end of the air pipe (40) extends to the inside of the culture chamber (8), so A high-definition camera (19) is provided on the top of the culture room body (1), a camera sealing protective cover (20) is provided outside the high-definition camera (19), and a cooling pipe (19) is provided below the high-definition camera (19). 2) A sprayer (3) is provided on one side of the cooling tube (2), a water inlet (18) is provided at one end of the cooling tube (2), and a support rod is provided in the middle of the cooling tube (2) (17) The water inlet pipe (7) is installed on one side of the culture chamber body (1), one end of the water inlet pipe (7) is provided with a water pump (8), and one side of the water pump (8) is provided with A control box (9), a PLC controller (10) is arranged inside the control box (9), a rotating shaft (15) is arranged in the middle of the blower (13), one side of the rotating shaft (15) A blade (14) is provided, one side of the blower (13) is provided with a cooling and heating regulator (16), the first temperature and humidity sensor (4) is installed on the inner wall of the left side of the culture chamber body (1), so Shupei The inner wall on the right side of the cultivation chamber body (1) is provided with a second temperature sensor (12), the bottom of the cultivation chamber body (1) is provided with an insulation pad (5), and the other inside the cultivation chamber body (1) A second culture chamber (24) is provided on the side, a fixed incubator (22) is provided inside the second culture chamber (24), and a base (26) is provided at the bottom end of the fixed incubator (22), so A temperature controller (27) is arranged on the upper right of the base (26), a tray (28) is arranged on the left side of the temperature controller (27), and the rotating motor (30) is installed below the tray (28) A fixing nut (29) is arranged on the lower two sides of the rotating electric machine (30), and a heating tube (31) is arranged below the rotating electric machine (30).
The microbial strain cultivation equipment according to claim 1, characterized in that: the outer side of the high-definition camera (19) is provided with a waterproof fog film.
The microbial strain cultivation equipment according to claim 1, characterized in that the rotating shafts (15) and (14) are connected by bolts.
The microbial strain culture equipment according to claim 1, characterized in that: the air outlet of the exhaust fan (36) is connected with a dust-proof net through a movable buckle.
The microbial strain culture equipment according to claim 1, wherein the top view of the tray (28) is circular.
The microbial strain culture equipment according to claim 1, characterized in that: the outer side of the control box (9) is provided with a heat sink.
The microbial strain culture equipment according to claim 1, wherein a partition is provided between the second culture chamber (24) and the first culture chamber (25), and the partition is connected to the culture chamber body ( 1) They are connected by bolts.