WO2021109509A1

WO2021109509A1 - Composition having immunity-enhancing function and preparation method therefor

Info

Publication number: WO2021109509A1
Application number: PCT/CN2020/094722
Authority: WO
Inventors: 肖伟; 范业文; 刘世娟; 王振东; 张海弢; 李曼曼; 柏伟荣
Original assignee: 江苏康仁医药科技开发有限公司
Priority date: 2019-12-02
Filing date: 2020-06-05
Publication date: 2021-06-10
Also published as: CN110893222A

Abstract

Provided is a composition having an immunity-enhancing function, comprising, in parts by weight: 1-12 parts of poria, 0.1-6 parts of Lycium chinense, 0.1-6 parts of semen coicis, 0.1-4 parts of ginseng, and 0.1-4 parts of Glycyrrhiza uralensis. The combined use of the five medicines can promote the cellular immunity and phagocytosis of mice, and has a significant effect of enhancing immunity.

Description

Composition with function of improving immunity and preparation method thereof

Technical field

The invention relates to the technical fields of medicines, health products and foods, and in particular to a composition with the function of improving immunity and a preparation method thereof.

Background technique

With the development of modern economy, the products developed with Chinese herbal medicine as raw materials with the extensive and profound culture of Chinese medicine as the connotation are deeply loved by people, and people's requirements for the efficacy of Chinese herbal medicine products are becoming higher and higher. Modern people have a fast-paced life, high life pressure, and a long-term "sub-health" state. Work efficiency, quality of life and learning will be affected to varying degrees. In severe cases, they will be prone to fatigue, susceptibility to colds, decreased sleep quality, dizziness and other symptoms. And eventually lead to a decline in immunity, and the low immunity of the body makes the "fatigue syndrome" more prominent and worse, thus forming a vicious circle and ultimately harming human health.

Summary of the invention

The invention intends to provide a Chinese herbal medicine composition, a drink and a preparation method thereof, which are aimed at sub-healthy people with low immunity, have a refreshing and pleasant mouthfeel, suitable for all seasons, and have a better immunity-improving effect.

The present invention proposes a composition with a function of improving immunity, comprising by weight: 1-12 parts of Poria cocos, 0.1-6 parts of wolfberry, 0.1-6 parts of Coix seed, 0.1-4 parts of ginseng, and 0.1-4 parts of licorice Copies. Wherein, the composition and the raw materials used can be directly ground into powder, or it can be an extract prepared by conventional means or other forms.

Further, the composition includes: 2-10 parts of Poria cocos, 0.5-6 parts of wolfberry, 0.5-6 parts of Coix seed, 0.2-3 parts of ginseng, and 0.2-3 parts of licorice.

Further, the composition includes: 3-8 parts of Poria cocos, 1-5 parts of Lycium barbarum, 1-5 parts of Coix seed, 0.5-2.5 parts of ginseng, and 0.5-2.5 parts of licorice.

Further, the composition includes: 4-8 parts of Poria cocos, 1-3 parts of wolfberry, 1-3 parts of coix seed, 0.5-2 parts of ginseng, and 0.5-2 parts of licorice.

Further, the composition includes: 8 parts of Poria cocos, 1 part of Chinese wolfberry, 1 part of Coix seed, 1 part of ginseng, and 1 part of licorice.

The present invention also proposes the application of any of the preceding compositions in the preparation of immunity-enhancing drugs, health products or foods.

The present invention also proposes a medicine, health care product or food with a composition capable of improving immunity as any of the previous items, which may include acceptable excipients in the medicine, health care product or food,

Further, the health food or food may be selected from beverages. Further, the beverage also includes one or more of white sugar, xylitol, sorbitol, and erythritol. Preferable are sorbitol and citric acid, and the ratio of sugar to acid is preferably 156:11-12.

The present invention also provides a method for preparing a health product or food as described above, and the preparation method includes the following steps:

(1) Extraction: take Poria cocos, wolfberry, coix seed, ginseng and licorice, add 8-12 times the amount of water to the total amount of medicinal materials, heat reflux and extract 1 to 3 times, extract 0.5 to 3 hours each time, filter, and combine the filtrate;

(2) Concentration: After the filtrate is allowed to stand in the refrigerator for 12 to 48 hours, the bottom sediment is centrifuged and combined with the supernatant, and concentrated under reduced pressure to obtain the concentrated solution, which is then left to stand in the refrigerator for 12 to 72 hours. Clarified concentrate;

(3) Preparation: Take the clarified concentrate in (2), add purified water to dilute and mix, then add correctives to prepare, dissolve, mix, and place in the refrigerator for 12 to 72 hours before filling and sterilization.

Specifically, the preparation method includes the following steps:

(1) Extraction: Take poria, wolfberry, coix seed ginseng (artificially planted) and licorice materials, remove impurities, and wash; add 8-12 times the total amount of water for the medicinal materials, reflux and heat to extract 1 to 3 times, each extraction Filter for 0.5 to 3 hours and combine the filtrate;

(2) Concentration: After the filtrate is allowed to stand in a refrigerator at 4°C for 12 to 48 hours, the bottom sediment is centrifuged (speed: 4000 to 6000 r/min, time: 5 to 20 min), then combined with the supernatant, and concentrated under reduced pressure to obtain The concentrated solution with a relative density of 1.00～1.10, again placed in a refrigerator at 4℃ for 12～72h, after high-speed centrifugation (rotation speed: 10000～18000r/min), a clear concentrated solution will be obtained for use;

(3) Blending: In the purification workshop, take the clarified concentrate in (2), add purified water to dilute and mix, the ratio of the clarified concentrate to purified water is 1:1～1:8, and then add the corrective ( Including sweeteners and acidity regulators), dissolve, mix, and stand in a refrigerator at 4°C for 12 to 72 hours.

(4) Filling and sterilization: In the purification workshop, take the liquid prepared in (3) and subject it to high-speed centrifugation (rotating speed: 10000～18000r/min), then fill and sterilize (heat to above 110～125℃, keep warm) Sterilize for 20～45min).

(5) Packaging: Sub-packaging and packaging are carried out in the outer packaging workshop.

More specifically, the preparation method includes the following steps:

Weigh 8 parts of Poria, 1 part of Lycium barbarum, 1 part of Coix Seed, 1 part of Ginseng, 1 part of Licorice;

(1) Extraction: take Poria cocos, wolfberry, coix seed, ginseng and licorice, add 10 times the amount of water to the total amount of medicinal materials, heat reflux for extraction 2 times, filter for 1 hour each time, and combine the filtrate;

(2) Concentration: After the filtrate is allowed to stand in a refrigerator at 4°C for 24h, the bottom sediment is centrifuged at 5000r/min for 10min and combined with the supernatant. After concentration under reduced pressure, a concentrated solution with a relative density of 1.55-1.65 is obtained. Leave it in the refrigerator for 48h, centrifuge at 14000r/min to obtain a clear concentrate, which is ready for use;

(3) Preparation: Take the clarified concentrate in (2), add purified water, the ratio of the clarified concentrate to purified water is 1:4, mix well, then add sorbitol and citric acid, mix, dissolve, mix, Put it in a refrigerator at 4℃ for 48h;

(4) Filling and sterilization: After centrifuging the prepared solution in (3) at 14000r/min, it is filled and sterilized. The sterilization conditions are heating to 115°C, heat preservation and sterilization for 30 minutes;

(5) Packaging: sub-packaging and packaging.

In the present invention, Poria, Lycium barbarum, Coix seed and licorice are all of the same medicine and food, and ginseng (artificially planted) is a new food raw material, which can be used for ordinary foods, which not only has food safety but also enhances the body's immunity. The beverage made by the composition of the present invention has a refreshing and pleasant taste, is suitable for all seasons, and is convenient for consumers to accept.

In the present invention, Poria cocos has a sweet and flat taste, enters the heart, lungs, and spleen channels, and can be used for edema, oliguria, phlegm, palpitations, spleen deficiency, low food, loose stools, diarrhea, restlessness, palpitations, and insomnia; medlar nourishes the kidney, nourishes the liver, Improve eyesight, strengthen muscles and bones, and relieve fatigue; Coix seed can be used for edema, athlete's foot, dysuria, diarrhea of spleen deficiency, etc.; Ginseng is returned to the spleen, lung meridian, and heart meridian, which can greatly invigorate vitality; the five drugs are used in combination, complementing each other, and the obtained solid The pharmacodynamic experiment of the drink has proved that it can significantly improve the phagocytic ability of mononuclear-macrophages in mice, enhance the transformation ability of mouse spleen lymphocytes, promote the cellular immunity and phagocytic ability of mice, and have a significant improvement in immunity effect.

Detailed ways

The following describes the present invention in more detail through specific embodiments, so as to better understand the solutions of the present invention and the advantages of various aspects thereof. However, the content of the specific embodiments described below is for illustrative purposes only, and is not a limitation to the present invention.

It should be noted that if the specific conditions are not specified, they will be carried out in accordance with the conventional conditions or the conditions recommended by the manufacturer. The raw materials or auxiliary materials used, and the reagents or instruments used without the manufacturer’s instructions are all commercially available. Regular products. Unless otherwise stated, all percentages, ratios, ratios or parts are by weight.

Unless otherwise defined, all professional and scientific terms used in the text have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the content described can be applied to the present invention.

Example 1

A drink for improving immunity, weighing raw materials according to the following weight ratio: Poria 60kg, Lycium chinense 20kg, Coix seed 20kg, Ginseng (artificially planted) 10kg, Licorice 10kg, Sorbitol 93.15kg, Citric acid 0.69kg. The specific preparation method is as follows:

(1) Extraction: Take poria, wolfberry, coix seed, ginseng (artificially planted) and licorice materials, remove impurities, and wash; add 10 times the amount of the total amount of the medicinal materials, heat and extract twice, and filter for 1 hour each time After that, the filtrates were combined.

(2) Concentration: After the filtrate is allowed to stand in a refrigerator at 4°C for 24 hours, the bottom sediment is centrifuged (speed: 5000r/min, time: 10min), then combined with the supernatant, and concentrated under reduced pressure to obtain a relative density of 1.55-1.65 The concentrated solution was placed again in a refrigerator at 4°C for 48h, and a clear concentrated solution was obtained after high-speed centrifugation (rotational speed: 14000r/min) for use.

(3) Preparation: In the purification workshop, take the clarified concentrate from (2), add purified water, the ratio of the clarified concentrate to purified water is 1:4, mix well, then add sorbitol and citric acid, mix and dissolve , Mix well, and let stand in a refrigerator at 4°C for 48h.

(4) Filling and sterilization: In the purification workshop, take the liquid prepared in (3) and centrifuge at high speed (rotation speed: 14000r/min), then fill and sterilize. The sterilization conditions are heating to 115°C, heat preservation and sterilization. Bacteria 30min.

(5) Packaging: Sub-packaging and packaging are carried out in the outer packaging workshop, that is, to improve the immunity of the drink.

Example 2

A drink for improving immunity, weighing raw materials according to the following weight ratio: Poria cocos 80 kg, wolfberry 15 kg, coix seed 15 kg, ginseng (artificial planting) 5 kg, licorice 5 kg, erythritol 93.15 kg, and citric acid 0.69 kg. The specific preparation method is as follows:

(3) Blending: In the purification workshop, take the clarified concentrate from (2), add purified water, the ratio of the clarified concentrate to purified water is 1:4, mix well, then add erythritol and citric acid to blend, Dissolve, mix, and let stand in a refrigerator at 4°C for 48h.

Example 3

A drink for improving immunity, weighing raw materials according to the following weight ratio: Poria cocos 40 kg, wolfberry 30 kg, coix seed 30 kg, ginseng (artificially planted) 10 kg, licorice 10 kg, sorbitol 93.15 kg, and citric acid 0.39 kg. The specific preparation method is as follows:

(2) Concentration: After the filtrate is placed in a refrigerator at 4°C for 24 hours, the bottom sediment is centrifuged (speed: 5000r/min, time: 10min), then combined with the supernatant, and concentrated under reduced pressure to obtain a relative density of 1.55-1.65 The concentrated solution was placed again in a refrigerator at 4°C for 48h, and a clear concentrated solution was obtained after high-speed centrifugation (rotational speed: 14000r/min) for use.

Example 4

A drink for improving immunity, weighing raw materials according to the following weight ratio: Poria cocos 80kg, wolfberry 10kg, coix seed 10kg, ginseng (artificially planted) 10kg, licorice 10kg, xylitol 93.15kg, and citric acid 0.69kg. The specific preparation method is as follows:

(3) Preparation: In the purification workshop, take the clarified concentrate from (2), add purified water, the ratio of the clarified concentrate to purified water is 1:4, mix well, then add xylitol and citric acid, mix, and dissolve , Mix well, and let stand in a refrigerator at 4°C for 48h.

Example 5

A drink for improving immunity. The raw materials are weighed according to the following weight ratio: Poria 60kg, Lycium barbarum 15kg, Coix seed 15kg, Ginseng (artificially planted) 15kg, Licorice 15kg, White sugar 93.15kg, and citric acid 0.69kg. The specific preparation method is as follows:

(2) Concentration: After the filtrate is allowed to stand in a refrigerator at 4°C for 24 hours, the bottom sediment is centrifuged (speed: 5000r/min, time: 10min), then combined with the supernatant, and concentrated under reduced pressure to obtain a relative density of 1.55-1.65 The concentrated solution was again placed in a refrigerator at 4°C for 48 hours, and then a clear concentrated solution was obtained after high-speed centrifugation (rotational speed: 14000r/min) for use.

(3) Preparation: In the purification workshop, take the clarified concentrate in (2), add purified water, the ratio of the clarified concentrate to purified water is 1:4, mix well, then add white sugar and citric acid, mix and dissolve , Mix well, and let stand in a refrigerator at 4°C for 48h.

Experimental example 1 Immunity enhancement function experiment

1 Experimental materials

1.1 Experimental animals and grouping

ICR mouse, clean grade, 18-22g, male.

In this experiment, immune group Ⅰ and immunization group Ⅱ were set up, and the blank group, the control group (0.16g/kg·BW, equivalent to 5 times the recommended amount of human body,), the experimental group (given the clarified concentrate prepared in Example 1 1.15 g/kg·BW (equivalent to 1g crude drug/kg·BW, 5 times the recommended amount of the human body), 10 per group; immune group I was used for the mouse lymphocyte transformation experiment, and immune group II was used for the carbon clearance experiment. The group and the experimental group were given intragastric administration at 1.0mL/kg·bw, and the blank group was given an equal volume of purified water once a day. After continuous administration for 30 days, various indexes were measured.

1.2 Drugs and reagents

1.2.1 Drugs

Positive control drug: Dendrobium candidum and American ginseng capsules (recommended dose 1.92g/d for human body).

Experimental group drug: the clarified concentrated liquid in Example 1 (the weight ratio of the medicinal materials to the clarified concentrated liquid is 1: 1.15).

1.2.2 Reagents

ConA, RPMI1640 culture medium, MTT, 0.1% Na ₂ CO ₃ solution, Indian ink.

1.3. Experimental instrument

Animal platform scales, ultra-clean workbenches, animal balances, electronic analytical balances, 722 spectrophotometers, CO ₂ incubators, microplate readers, sterile surgical instruments, cell counters, cell culture plates, disposable syringes.

2 Experimental method

2.1 ConA-induced mouse splenic lymphocyte transformation experiment (MTT method)

Take the spleen aseptically to prepare a cell suspension, adjust the cell concentration to 3×10 ⁶ cells/mL. The cell suspension was added to a 24-well culture plate in two wells, 1 mL per well, 75 μL of ConA solution was added to one hole, and the other hole was used as a control, _{and cultured in a 5% CO 2} , 37° C. incubator for 72 hours. Remove 0.7mL of the supernatant, add 0.7mL of RPMI1640 culture medium without calf serum, add 50μL/well of MTT, continue to incubate for 4h, add 1mL of acidic isopropanol, and dissolve it into a 96-well culture plate. Three parallel, 570nm microplate reader to measure the optical density (OD).

2.2 Mouse carbon clearance experiment

Mice were injected with Indian ink diluted 4 times with normal saline into the tail vein, and timed immediately after ink was injected. At 2 and 10 minutes after the ink was injected, 20 μL of blood was taken from the medial canthal vein and added to ₂ mL of 0.1% Na 2 CO ₃ solution, and the optical density (OD) was measured at a wavelength of 600 nm. The mice were sacrificed, the liver and spleen were weighed, and the phagocytic index was calculated.

Phagocytosis index a = end weight × K ^1/3 (liver weight + spleen weight)

K=(lgOD _2min lgOD _10min )/(10min-2min)

2.3 Experimental data statistics: use SPSS11.0 software for statistical analysis.

3 experimental results

3.1 ConA-induced mouse splenic lymphocyte transformation experiment

Table 1 Splenic lymphocyte transformation of mice in each group

It can be seen from Table 1 that the proliferation ability of spleen lymphocytes of the control group and the experimental group is significantly different from that of the blank group (P<0.05).

3.2 Mouse carbon clearance experiment

Table 2 Mouse monocyte-macrophage carbon clearance phagocytic index in each group

It can be seen from Table 2 that the mononuclear-macrophage carbon clearance phagocytic index of mice in the control group and the experimental group is significantly different from that of the blank group (P<0.05).

Based on the above experimental results, it is shown that the present invention can significantly improve the phagocytic ability of mononuclear-macrophages in mice, and can enhance the transformation ability of mouse spleen lymphocytes, indicating that the present invention has the function of enhancing immunity.

Experimental Example 2 Investigation of the ratio of sweetener and sugar acid in beverage

In order to make the drink of the present invention have a suitable taste, it is necessary to add a certain amount of sweetener, and the formation of the flavor of the drink is produced by the combination of the sweetener and the Chinese medicinal material extract, and the appropriate sweetness can cover the bitter taste of the Chinese medicinal material , Make the taste more pleasant.

1. Sensory evaluation criteria for beverages

To ensure the accuracy and feasibility of the sensory evaluation, a total of 10 judges, 5 males and 5 females, are judged. The judges have a certain professional foundation and good taste acuity. Comprehensive evaluation is carried out according to color, smell, taste, tissue state, and impurities, and the average is taken Points are scored for their senses.

Table 3 Sensory Evaluation Criteria

2. Investigation of the dilution factor of the clarified concentrate

Take the clarified concentrated liquid in Example 1, 100g each, 6 parts in total, according to the ratio of clarified concentrated liquid to purified water of 1:1, 1:2, 1:3, 1:4, 1:5 and 1:6 Dilute, mix well, and perform sensory evaluation.

Table 4 Sensory scores of dilution multiples of clarified concentrates

The experimental results show that when the ratio of clarified concentrated liquid to purified water is 1:4, the sensory score is the highest, the characteristic flavor is appropriate, and will not be too thick or too weak; when the ratio of clarified concentrated liquid to purified water is 1:1 to 3, the characteristic flavor Too strong, the bitterness is strong; when the ratio of clarified concentrate to purified water is 1:5-6, the characteristic flavor is too weak, and the taste is weak. Therefore, the ratio of clarified concentrate to purified water is 1:4.

3. Sweetener screening

Take the clarified concentrated solution in Example 1 and dilute it according to the ratio of clarified concentrated solution to purified water of 1:4. Take 4 portions, each 100g, and add 15.6g white sugar, xylitol, sorbitol, and erythritol. The sugar alcohol is stirred until it is completely dissolved, and the sensory evaluation is performed.

Table 5 Sensory scores of drinks prepared with different sweeteners

The experimental results show that sorbitol has the highest sensory evaluation score and the most pleasant taste. The sensory scores of drinks added with white sugar and xylitol are the lowest, because their sweetness is too high and the sweetness is too strong; the sensory scores of erythritol and sorbitol are similar, but the price of erythritol is higher, which is to control the cost Therefore, sorbitol was chosen as a sweetener.

4. Inspect the ratio of sugar and acid

Take the clarified concentrated solution in Example 1 and dilute it according to the ratio of clarified concentrated solution to purified water of 1:4, take 5 portions, each 1000g, first add 156g sorbitol and stir until it is completely dissolved, then add 10.0g respectively , 11.0g, 11.5g, 12.0g, 13.0g, stir until completely dissolved, and perform sensory evaluation.

Table 6 Sensory scores of drinks prepared with different sugar and acid ratios

The experimental results show that the citric acid addition amount is 11.0～12.0g, the sensory evaluation score is higher, so 156:11～12 is selected as the better sugar-acid ratio.

Based on the above results, the preferred sweetener is sorbitol, and the preferred sugar-to-acid ratio is 156:11-12.

The description of the above embodiments is only used to help understand the method and core idea of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

A composition with a function of improving immunity, characterized in that it comprises by weight: 1-12 parts of Poria cocos, 0.1-6 parts of wolfberry, 0.1-6 parts of Coix seed, 0.1-4 parts of ginseng, and 0.1-4 parts of licorice Copies.
The composition according to claim 1, characterized in that it comprises: 2-10 parts of Poria cocos, 0.5-6 parts of wolfberry, 0.5-6 parts of coix seed, 0.2-3 parts of ginseng, and 0.2-3 parts of licorice.
The composition according to claim 1, characterized in that it comprises 3 to 8 parts of Poria, 1 to 5 parts of Lycium barbarum, 1 to 5 parts of Coix Seed, 0.5 to 2.5 parts of Ginseng, and 0.5 to 2.5 parts of Licorice.
The composition according to claim 1, characterized in that it comprises: 4 to 8 parts of Poria cocos, 1 to 3 parts of wolfberry, 1 to 3 parts of coix seed, 0.5 to 2 parts of ginseng, and 0.5 to 2 parts of licorice.
The use of the composition of any one of claims 1 to 4 in the preparation of immunity-enhancing drugs, health products or foods.
A medicine, health care product or food containing a composition with the function of improving immunity according to any one of claims 1-4.
The health food or food according to claim 6, wherein the health food or food is selected from beverages.
The health product or food according to claim 7, wherein the beverage comprises one or more of white sugar, xylitol, sorbitol, and erythritol.
A method for preparing health care products or foods according to claim 7, wherein the preparation method comprises the following steps:

(1) Extraction: Take Poria cocos, wolfberry, coix seed, ginseng and licorice, add 8-12 times the amount of water to the total amount of medicinal materials, heat reflux and extract for 1 to 3 times, filter for 0.5 to 3 hours each time, and combine the filtrate;

(2) Concentration: After the filtrate is allowed to stand in the refrigerator for 12 to 48 hours, the bottom sediment is centrifuged and combined with the supernatant, and concentrated under reduced pressure to obtain the concentrated solution, which is then left to stand in the refrigerator for 12 to 72 hours. Clarified concentrate;

(3) Blending: Take the clarified concentrate in (2), add purified water to dilute and mix, then add correctives to blend, dissolve, mix, and place it in the refrigerator for 12 to 72 hours before filling and sterilizing.
A method for preparing health care products or foods according to claim 9, characterized in that, the preparation method comprises the following steps:

Weigh 8 parts of Poria, 1 part of Lycium barbarum, 1 part of Coix Seed, 1 part of Ginseng, 1 part of Licorice;

(1) Extraction: take Poria cocos, wolfberry, coix seed, ginseng and licorice, add 10 times the amount of water to the total amount of medicinal materials, heat reflux for extraction 2 times, filter for 1 hour each time, and combine the filtrate;

(2) Concentration: After the filtrate is allowed to stand in a refrigerator at 4°C for 24h, the bottom sediment is centrifuged at 5000r/min for 10min and combined with the supernatant. After concentration under reduced pressure, a concentrated solution with a relative density of 1.55-1.65 is obtained. Leave it in the refrigerator for 48h, centrifuge at 14000r/min to obtain a clear concentrate, which is ready for use;

(3) Preparation: Take the clarified concentrate in (2), add purified water, the ratio of the clarified concentrate to purified water is 1:4, mix well, then add sorbitol and citric acid, mix, dissolve, mix, Put it in a refrigerator at 4℃ for 48h;

(4) Filling and sterilization: After centrifuging the prepared solution in (3) at 14000r/min, it is filled and sterilized. The sterilization conditions are heating to 115°C, heat preservation and sterilization for 30 minutes;

(5) Packaging: sub-packaging and packaging.