WO2021066358A1 - Honeybee feeding solution composition for improving honey collection, and preparation method therefor - Google Patents

Honeybee feeding solution composition for improving honey collection, and preparation method therefor Download PDF

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Publication number
WO2021066358A1
WO2021066358A1 PCT/KR2020/012519 KR2020012519W WO2021066358A1 WO 2021066358 A1 WO2021066358 A1 WO 2021066358A1 KR 2020012519 W KR2020012519 W KR 2020012519W WO 2021066358 A1 WO2021066358 A1 WO 2021066358A1
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Prior art keywords
extract
composition
seaweed
propolis
feeding liquid
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PCT/KR2020/012519
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French (fr)
Korean (ko)
Inventor
하성섭
장순초
박종용
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주식회사 바이오포스
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Priority claimed from KR1020190120748A external-priority patent/KR102089736B1/en
Priority claimed from KR1020190165656A external-priority patent/KR102149215B1/en
Application filed by 주식회사 바이오포스 filed Critical 주식회사 바이오포스
Publication of WO2021066358A1 publication Critical patent/WO2021066358A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms

Definitions

  • the present invention relates to a composition for bee feeding liquid for improving watertightness and a method for producing the same, and more particularly, to an environment-friendly composition for bee feeding liquid and a method for producing the same, which can increase the productivity of beekeepers by increasing the immunity of bees. It is about.
  • Beekeeping is traditionally used not only for the production of honey and beeswax, but also for the production of royal jelly, pollen, propolis, and bee venom. It is used in a variety of ways to the use of bees for (bee pollination). Although the ratio of honey production to the total livestock production is not very large, it is known that 70% of the food we eat is pollinated by bees. Therefore, maintaining bee populations will have a profound impact on agricultural production.
  • the most important thing in the management of beekeeping is to maintain the strength of the beekeeper as a strong force. If the Bonggun is strong, bees can have immunity against diseases, improve watertightness, and prevent the bee from overwintering in winter. In order to make the Bonggun strong, first, spawning in spring must be managed, pesticide damage must be minimized in summer, and the occurrence of viral diseases must be minimized by managing the humidity inside the beehive. In addition, it is very important to reduce the mortality of bees by preventing old horse disease and controlling mites after harvesting of the major domestic wheat sources such as ash and chestnut honey.
  • Feeding management in autumn for wintering is to prepare enough food and train young worker bees to prepare for wintering and to secure a bee farm that is advantageous for wintering.
  • drug groups with less than 5,000 animals
  • the most important factor in maintaining the strength of the Bonggun as a strong force is the improvement of the spawning rate, which should be maintained throughout the year as well as in the spring and autumn seasons.
  • the present invention aims to improve the immunity of bees by preparing a composition for feeding liquid containing seaweed complex extract, wormwood extract, and propolis extract as active ingredients.
  • an object of the present invention is to increase the productivity of beekeepers by increasing the number of bees and the number of spawning of queen bees through an eco-friendly composition derived from plant extracts.
  • the present invention comprises a seaweed complex extract, a wormwood extract, a propolis extract, citric acid, sodium L-glutamate, and vegetable oils, and the seaweed complex extract, a wormwood extract, and a propolis extract are 40 It provides a composition for bee feeding liquid, characterized in that hot water extraction for 4 to 8 hours at ⁇ 60 °C.
  • composition for the feeding solution may be used by diluting 1/100 to 1/1000 in the feeding solution.
  • the seaweed complex extract may be included in an amount of 0.1 to 5% by weight based on the total composition.
  • the wormwood extract may be included in an amount of 0.1 to 0.5% by weight based on the total composition.
  • the propolis extract may be included in an amount of 0.1 to 1% by weight based on the total composition.
  • the citric acid may be included in an amount of 0.5 to 3% by weight, the sodium L-glutamate in an amount of 0.1 to 1% by weight, and the vegetable oil in an amount of 0.05 to 1.5% by weight, based on the total composition.
  • the present invention comprises the steps of (a) mixing seaweed, green laver, and laver and extracting hot water at 40 to 60° C. for 4 to 8 hours to prepare a seaweed complex extract; (b) preparing a wormwood extract by hot water extraction at 40 to 60 °C for 4 to 8 hours; (c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And (d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, wormwood extract, and propolis extract. Provides a way.
  • the seaweed complex extract may be extracted by mixing seaweed, green laver, laver and water in a weight ratio of 1:10 to 100.
  • the seaweed, green laver, and seaweed may be mixed in a weight ratio of 1:1:1 or 2:1:1 or 1:2:1 or 1:1:2.
  • step (b) the wormwood may be mixed with water in a weight ratio of 1:10 to 100, and in step (c), propolis may be mixed with water in a weight ratio of 1:10 to 100.
  • composition for feeding liquid according to the present invention has the advantage of improving the immunity of bees environmentally by using natural products derived from plant extracts.
  • the productivity of beekeeping products can be improved by increasing the number of bees and the number of spawning by increasing the immunity of bees.
  • Example 1 is a graph showing the temperature change after feeding the composition for feeding liquid of Examples 1, 2, and Comparative Example 1 to bees.
  • Example 2 is a graph showing changes in the number of individuals after feeding the composition for feeding liquid of Examples 1, 2, and Comparative Example 1 to bees over time.
  • Example 3 is a graph showing changes in the number of spawning over time after the composition for feeding solutions of Examples 1, 2, and Comparative Example 1 is delivered to a queen bee.
  • the present invention comprises seaweed complex extract, wormwood extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oils, and the seaweed complex extract, wormwood extract, and propolis extract are at 40 to 60°C. It provides a composition for bee feeding liquid, characterized in that hot water extraction for 8 hours.
  • the present invention comprises the steps of (a) mixing seaweed, green laver, and laver and extracting hot water at 40 to 60° C. for 4 to 8 hours to prepare a seaweed complex extract; (b) preparing a wormwood extract by hot water extraction at 40 to 60 °C for 4 to 8 hours; (c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And (d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, wormwood extract, and propolis extract. Provides a way.
  • the present invention comprises seaweed complex extract, wormwood extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oils, and the seaweed complex extract, wormwood extract, and propolis extract are at 40 to 60°C. It provides a composition for bee feeding liquid, characterized in that hot water extraction for 8 hours.
  • Seaweed is a macroalgae that lives in the ocean and refers to a multicellular protozoa that grows in the sea, and is distinguished from single-celled microalgae in that it has a size that can be observed with the naked eye.
  • the classification within seaweed is divided into green seaweed, brown seaweed, and red seaweed according to the photosynthetic pigment, and there are differences in their habitat environment and composition.
  • the green algae include green, auditory, blue, and the like, and are grown in the ocean or fresh water.
  • the red algae include agar, laver, karanigin, and the like, and grow in the ocean.
  • Brown algae include tot, seaweed, kelp, rhubarb, and mackerel, and the brown algae particularly contain up to about 30-67% carbohydrates on a dry matter basis.
  • the polysaccharides contained in brown algae are mostly composed of alginic acid.
  • Alginic acid is a hetero-type polysaccharide in which ⁇ -D-mannuronic acid (M) and ⁇ -L-guluronic acid (G) are ⁇ -1, 4 bonded or ⁇ -1,4 bonded as the main component of the cell membrane or intercellular material of brown algae.
  • M ⁇ -D-mannuronic acid
  • G ⁇ -L-guluronic acid
  • the seaweed complex extract according to an embodiment of the present invention contains alginic acid as an active ingredient.
  • Alginic acid enhances the production of nitric oxide and the spread of granular leukocytes, so it can improve the immunity of bees.
  • the alginic acid may be contained in an amount of 7.5 to 500 mg/kg compared to the seaweed complex extract, and if it is out of the above range, the effect of enhancing the immunity of bees due to alginic acid is insignificant, so it is preferable to be included in the above range.
  • the seaweed complex extract may be prepared by mixing seaweed, green seaweed, and seaweed, and the seaweed, green seaweed, and seaweed 1:1:1 or 2:1:1 or 1:2 It is preferably prepared by mixing in a weight ratio of :1 or 1:1:2. Since alginic acid can be extracted within an effective range only when it is mixed in the weight ratio, it is preferable to mix it in the weight ratio.
  • an extraction solvent for extracting the extract water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used.
  • the mixed solvent is not particularly limited, but it is preferable to use water.
  • the seaweed complex extract may be prepared by mixing seaweed, green laver, and seaweed in a weight ratio of 1:10 to 100 with water and extracting for 4 to 8 hours at 40 to 60°C.
  • the active ingredient of the extract may be extracted in a small amount.
  • the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
  • the kelp (Laminaria japonica) is a seaweed belonging to the Phaeophyta, Phaeophyceae) Kelp tree (Laminariales) Kelp family (Laminariaceae), and is divided into upper leaves, stems, and roots in appearance, and the upper leaves are bands. It is long in shape, and the central part of the upper part of the leaf forms a thick middle part, and both sides of the middle part have vertical flaws.
  • the stem is usually columnar and erect, and the root has a dendritic form branching from the lower part of the stem to the second phase. Since ancient times, the kelp has been widely used for food in Japan, China, including Korea.
  • the seaweed (Undaria pinnatifida) is a seaweed belonging to the algae family of Phaeophyta, Phaeophyceae) Kelp tree (Laminariales) Seaweed family (Alariaceae), the base is dendritic, the stem is oblate, and the upper part Is the middle of the upper leaf, and the leaf has the right upper lobe on the jaw. It is distributed in Korea and Japan. In Korea, it is distributed in a wide area from Baengnyeongdo Island on the west coast to the end of the east coast. It is cultivated in the southern coastal areas such as Busan and Wando, and has been edible in Korea and Japan since ancient times.
  • the green onion is a seaweed belonging to the Galparaeaceae family, and it is rich in iron, methylmethionine, dietary fiber and chlorophyll components, and is effective in improving anemia, neutralizing nicotine, improving constipation, dieting and removing bad breath.
  • Green lava is resistant to pollution and grows well in eutrophied environments, and unlike land plants, it contains a large amount of polysaccharide containing a sulfuric acid group, and is mainly composed of glucurono-rhamnoxyloglycan. It contains about 20% and 21% of sulfuric acid and uronic acid, respectively.
  • the seaweed complex extract according to an embodiment of the present invention may be included in an amount of 0.1 to 5% by weight relative to the composition for the total specification solution.
  • Artemisia annua refers to the annual herb of the Chrysanthemum family of the Chrysanthemum family, which is called fine leaf mugwort, dog mugwort, or blue mugwort. It grows to about 1m in roadsides, vacant lands, and riversides, and is called Cheongho because the back side of the leaf is blue among mugwort. In oriental medicine, it is known to be effective in treating fever, cold, dysentery, childhood, indigestion and dysentery.
  • Artemisinin a polyphenol compound that has antioxidant, anti-inflammatory, anti-cancer, and immunomodulatory effects, is contained in the wormwood.
  • it is known to contain 50-70% of oregano, which is known as the most excellent material for its antioxidant activity.
  • a polyphenol is included as an active ingredient, and the polyphenol may be contained in an amount of 0.01 to 1000 mg/kg compared to the wormwood extract.
  • the polyphenol has antioxidant, antibacterial, and antibiotic effects, and helps relieve stress, thereby enhancing the immunity of honey.
  • an extraction solvent for extracting the extract water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used.
  • the mixed solvent is not particularly limited, but it is preferable to use water.
  • the wormwood extract may be prepared by mixing the wormwood with water in a weight ratio of 1:10 to 100 and extracting for 4 to 8 hours at 40 to 60°C.
  • the active ingredient of the extract may be extracted in a small amount.
  • the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
  • the wormwood extract according to an embodiment of the present invention may be included in an amount of 0.1 to 0.5% by weight relative to the composition for the total specification solution.
  • Propolis is a material made by mixing saliva and enzymes with resin-like materials extracted from various plants by bees for survival and reproduction. It contains flavonoids and the like.
  • Propolis is a natural antibiotic and is known to contain the most organic matters and minerals (inorganic salts), along with 104 kinds of ingredients.
  • As its main active ingredients flavonoids, polyphenols, aromatic carboxylic acids, aromatic aldehydes, etc. It is known and its main actions include antibacterial action, antioxidant action, anti-inflammatory action, local anesthetic action, viral proliferation inhibitory action, and immune regulation action.
  • the propolis extract according to an embodiment of the present invention may contain polyphenol as an active ingredient, and the polyphenol may be contained in an amount of 0.001 to 1000 mg/kg compared to the propolis extract.
  • the polyphenol has antioxidant, antibacterial, and antibiotic effects, and helps relieve stress, thereby enhancing the immunity of honey.
  • an extraction solvent for extracting the extract water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used.
  • the mixed solvent is not particularly limited, but it is preferable to use water.
  • the propolis extract may be prepared by mixing propolis with water in a weight ratio of 1:10 to 100 and extracting at 40 to 60°C. When the weight ratio of the propolis and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
  • the propolis extract according to an embodiment of the present invention may be included in an amount of 0.1 to 1% by weight based on the total composition.
  • the mixture of the seaweed complex extract, wormwood extract, and propolis extract may be contained in an amount of 0.3 to 6.5% by weight compared to the composition for the total specification solution.
  • the content of the seaweed complex extract, wormwood extract, and propolis extract is less than 0.3% by weight of the total composition, it is difficult to produce the desired effect in enhancing the immunity of honey, and when the content exceeds 6.5% by weight, it is effective compared to the input of the raw material. It is not economically desirable because the improvement of is minimal.
  • composition for feeding liquid according to the present invention may further include citric acid, sodium L-glutamate, and vegetable fats in addition to the active ingredients.
  • the citric acid may be included in an amount of 0.5 to 3% by weight of the total composition
  • the sodium L-glutamate may be included in an amount of 0.1 to 1% by weight
  • the vegetable oil may be included in an amount of 0.05 to 1.5% by weight.
  • the composition for the feeding solution may be used by diluting 1/100 to 1/1000 in the feeding solution.
  • the composition for feeding liquid may be used with different dilution concentrations depending on the application. For example, when it is used for making a strong group or for wintering, it is preferable to mix 500 ml of the non-freezing composition with 100 l of the non-freezing solution, and dilute it to 1/200.
  • the present invention comprises the steps of: (a) mixing seaweed, green laver, and laver and extracting hot water at 40 to 60° C. for 4 to 8 hours to prepare a seaweed complex extract; (b) preparing a wormwood extract by hot water extraction at 40 to 60 °C for 4 to 8 hours; (c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And (d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, wormwood extract, and propolis extract. Provides a way.
  • the seaweed complex extract may be prepared by mixing seaweed, green laver, and laver, and the seaweed, green laver, and laver 1:1:1 or 2:1:1 or 1:2:1 or It is preferably prepared by mixing in a weight ratio of 1:1:2.
  • the extraction solvent for extracting the extract in step (a) water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used.
  • the mixed solvent is not particularly limited, but it is preferable to use water.
  • the seaweed complex extract may be prepared by mixing seaweed, green laver, and seaweed in a weight ratio of 1:10 to 100 with water and extracting for 4 to 8 hours at 40 to 60°C.
  • the active ingredient of the extract may be extracted in a small amount.
  • the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
  • the extraction solvent for extracting the extract in step (b) water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used.
  • the mixed solvent is not particularly limited, but it is preferable to use water.
  • the wormwood extract may be prepared by mixing the wormwood with water in a weight ratio of 1:10 to 100 and extracting for 4 to 8 hours at 40 to 60°C.
  • the active ingredient of the extract may be extracted in a small amount.
  • the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
  • the extraction solvent for extracting the extract in step (c) water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used.
  • the mixed solvent is not particularly limited, but it is preferable to use water.
  • the propolis extract may be prepared by mixing propolis with water in a weight ratio of 1:10 to 100 and extracting for 4 to 8 hours at 40 to 60°C.
  • the active ingredient of the extract may be extracted in a small amount.
  • the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
  • citric acid may be added in an amount of 0.5 to 3% by weight relative to the total composition
  • the sodium L-glutamate may be added in an amount of 0.1 to 1% by weight
  • the vegetable oil may be added in an amount of 0.05 to 1.5% by weight.
  • the composition for a feeding solution prepared by the above step can be used by diluting 1/100 to 1/1000 in a feeding solution.
  • the composition for feeding liquid may be used with different dilution concentrations depending on the application. For example, in the case of making a strong group and using it for wintering specifications, it is preferable to mix 500 ml of the non-freezing composition with 100 l of the non-freezing solution, and dilute it to 1/200.
  • composition for feeding liquid according to the present invention has the advantage of improving the immunity of bees environmentally by using natural products derived from plant extracts.
  • the productivity of beekeeping products can be improved by increasing the number of bees and the number of spawning by increasing the immunity of bees.
  • seaweed complex extract In order to manufacture the seaweed complex extract, all domestic seaweed, green laver, and seaweed were purchased as living organisms. In order to remove salt and foreign substances from the seaweed, green seaweed, and seaweed, it was washed with running water three times or more, then first frozen and freeze-dried. Thereafter, the weight ratio of seaweed, green seaweed, and seaweed was mixed to be 1:1:1. The mixture and water were mixed to be 10% (w/w), immersed for 24 hours, extracted with stirring at 50° C. for 6 hours, and filtered to prepare an extract.
  • the leaves of the wormwood were washed with water, mixed so that the leaves and water were 10% (w/w), extracted with stirring at 50° C. for 6 hours, and filtered to prepare a wormwood extract.
  • the propolis powder and water were mixed so as to be 10% (w/w), extracted with stirring at 50° C. for 6 hours, and then filtered to prepare a propolis extract.
  • the prepared composition for specification solution was diluted 1/200 in 30% sugar water of Comparative Example 1 and added.
  • composition for a feeding solution prepared by the same method as in Example 1 was diluted 1/250 in 30% sugar water of Comparative Example 1 and added.
  • Example 1 and 2 and Comparative Example 1 were fed to bees for 20 minutes over three times at 0 am, 4 am, and 8 am, and the temperature change after feeding is shown in FIG. 1.
  • Example 1 and 2 and Comparative Example 1 were fed to bees for 20 minutes over three times at 0 am, 4 am, and 8 am, and the change in the number of individuals after feeding is shown in FIG. 2.
  • Example 2 the increase in the number of individuals was 7.3% in Comparative Example 1, 35.1% in Example 1, and 15.9% in Example 2. It could be confirmed that feeding by adding honey was helpful to increase the number of bees.
  • Example 2 in which the composition for feeding solution was diluted 250 times. After 15 days passed, it could be confirmed that the number of individuals decreased.
  • Example 1 in which the composition for feeding solution was diluted 200 times, the number of bees gradually increased as time passed, and as a result, it was confirmed that the number of bees increased by 35.1%.
  • composition according to the present invention by diluting 200 times in the feeding solution, and in the season when there is no effect on the increase in the number of individuals, diluting the dilution factor by more than 250 times is effective for feeding management I could confirm.
  • Examples 1 and 2 and Comparative Example 1 were fed for 20 minutes over three times at 0 am, 4 am, and 8 am and delivered to the queen bee. .
  • the spawning number of queen bees was measured in empty consumption after removing pupae and larvae from all honeycomb consumption before the measurement day.
  • Example 1 Compared to Comparative Example 1, the number of scattering in Example 1 was increased by 40.6%, and the number of scattering in Example 2 was increased by 5.7%. Therefore, it was confirmed that feeding by adding the composition for feeding solution according to the present invention rather than feeding the feeding solution without any treatment was helpful in increasing the spawning number of the queen bee.
  • Example 1 which was diluted 200 times and used, compared to Example 2, which was used after diluting the composition for a feeding solution 250 times. Therefore, it was confirmed that it is most preferable to use the composition according to the present invention by diluting it 200 times in the feeding solution at a time when a lot of spawning is required for feeding management.
  • composition for a bee feeding solution and a method for preparing the same according to an embodiment of the present invention have been described, but it is obvious that various embodiments can be modified without departing from the scope of the present invention.
  • composition for feeding liquid according to the present invention can enhance the immunity of bees environmentally by using natural products derived from plant extracts.
  • the productivity of beekeeping products can be improved by increasing the number of bees and the number of spawning by increasing the immunity of bees.

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Abstract

The purpose of the present invention is to prepare a feeding solution composition containing, as active ingredients, a seaweed composite extract, an Artemisia annua extract and a propolis extract, so as to improve the immunity of honeybees through a natural product derived from nature, and thus increase the population of honeybees and the egg yield of queen bees, thereby improving productivity of beekeeping farmers. The present invention provides a honeybee feeding solution composition comprising a seaweed composite extract, an Artemisia annua extract, a propolis extract, citric acid, sodium L-glutamate and vegetable oil, wherein the seaweed composite extract, the Artemisia annua extract and the propolis extract are obtained by performing hot water extraction at 40-60°C for 4-8 hours.

Description

수밀력 향상을 위한 꿀벌 사양액용 조성물 및 그의 제조방법Composition for bee feeding liquid for improving watertightness and its manufacturing method
본 발명은 수밀력 향상을 위한 꿀벌 사양액용 조성물 및 그의 제조방법에 관한 것으로, 더욱 상세하게는 꿀벌의 면역력을 증가시켜 양봉농가의 생산성을 증대시킬 수 있는 환경 친화적인 꿀벌 사양액용 조성물 및 그의 제조방법에 관한 것이다. The present invention relates to a composition for bee feeding liquid for improving watertightness and a method for producing the same, and more particularly, to an environment-friendly composition for bee feeding liquid and a method for producing the same, which can increase the productivity of beekeepers by increasing the immunity of bees. It is about.
양봉은 전통적으로 벌꿀(honey)과 밀랍(bee wax) 생산뿐만 아니라, 왕유(royal jelly), 화분(pollen), 봉교(propolis), 봉독(bee venom) 등의 생산에 이용되거나, 농작물의 화분매개(bee pollination)에 꿀벌을 이용하기까지 다양하게 활용되고 있다. 벌꿀 생산액이 전체 축산업 생산액에서 차지하는 비율은 그리 크지는 않지만, 우리가 먹는 음식의 70 %가 꿀벌에 의하여 수분이 되는 것으로 알려져 있다. 따라서 꿀벌의 개체수를 유지하는 것은 농업 생산에 지대한 영향을 미칠 것이다.Beekeeping is traditionally used not only for the production of honey and beeswax, but also for the production of royal jelly, pollen, propolis, and bee venom. It is used in a variety of ways to the use of bees for (bee pollination). Although the ratio of honey production to the total livestock production is not very large, it is known that 70% of the food we eat is pollinated by bees. Therefore, maintaining bee populations will have a profound impact on agricultural production.
국내 양봉 산업은 꿀벌 산물의 국내 시장이 약 4,000억 규모에 달하며, 농작물의 화분매개 등 벌의 생태계 다원적 기능은 계량화하기 어려울 정도로 크다. 국내에서 꿀벌에 의한 경제적 가치는 전체 과채류 생산액인 12.5조원 중 50 %인 6조원을 담당하고 있으며, 전체 생태계 보존효과는 50조원을 초과한다. 꿀벌은 공장식으로 밀집되어 사육되는 대표적인 가축이다. 농가에 따라서 벌통이 수 백 통에서부터 수 천 통까지 이르며 우리나라의 경우 봉군 수가 세계 20위로 45명당 1개의 봉군 비를 이루고 있다. 국내에는 2011년 기준으로 약 200만 개의 봉군이 있는 것으로 알려져 있다.In the domestic beekeeping industry, the domestic market of bee products reaches about 400 billion won, and the ecological plural functions of bees such as pollen mediation of agricultural crops are so large that it is difficult to quantify. The economic value of honeybees in Korea accounts for 50% of the total fruit and vegetable production of 12.5 trillion won, or 6 trillion won, and the overall ecosystem preservation effect exceeds 50 trillion won. Bees are a representative livestock that is concentrated and reared in a factory style. Depending on the farm, the number of beehives ranges from several hundred to thousands, and in Korea, the number of beehives is the 20th in the world, making up one beehive per 45 people. It is known that there were about 2 million rebels in Korea as of 2011.
양봉의 사양관리에서 가장 중요한 것은 봉군의 강세를 강군으로 유지시키는 것이다. 봉군의 강세가 강군일 경우 꿀벌은 질병에 대해 면역력을 가질 수 있고, 수밀력이 향상되며, 겨울철 월동시 동사를 방지할 수 있다. 봉군의 강세를 강군으로 만들기 위해서는 첫번째로 봄철 산란을 관리해야 하며 여름철 농약피해를 최소화시키고, 벌통 내부의 습도를 관리하여 바이러스 질병의 발생을 최소화시켜야 한다. 또한, 국내 주요 밀원인 아까시, 밤꿀 등의 채밀이 끝난 후 노제마병을 예방하고 응애를 방제하여 꿀벌의 폐사를 줄이는 것이 매우 중요하다. The most important thing in the management of beekeeping is to maintain the strength of the beekeeper as a strong force. If the Bonggun is strong, bees can have immunity against diseases, improve watertightness, and prevent the bee from overwintering in winter. In order to make the Bonggun strong, first, spawning in spring must be managed, pesticide damage must be minimized in summer, and the occurrence of viral diseases must be minimized by managing the humidity inside the beehive. In addition, it is very important to reduce the mortality of bees by preventing old horse disease and controlling mites after harvesting of the major domestic wheat sources such as ash and chestnut honey.
월동을 위한 가을철 사양관리로는 충분한 먹이를 준비하는 것과 젊은 일벌을 양성하여 월동을 대비하고 월동에 유리한 양봉장을 확보하는 것이다. 월동 실패의 원인이 몇 가지 있으나 그 중 약군(5,000 마리 이하)의 경우 봉군 내 온도를 조절하지 못해 준비한 식량을 남기고 동사하는 경우가 많다. 따라서, 봉군의 강세를 강군으로 유지하기 위한 가장 중요한 요인은 산란율 향상이며, 이는 봄, 가을철 뿐만 아니라 1년 내내 유지되어야 한다. Feeding management in autumn for wintering is to prepare enough food and train young worker bees to prepare for wintering and to secure a bee farm that is advantageous for wintering. There are several causes of wintering failure, but among them, drug groups (with less than 5,000 animals) are often unable to control the temperature in the Bonggun, leaving behind prepared food. Therefore, the most important factor in maintaining the strength of the Bonggun as a strong force is the improvement of the spawning rate, which should be maintained throughout the year as well as in the spring and autumn seasons.
그러나 국내 양봉 농가에서 사용하고 있는 산란율 향상 방법은 한약재를 이용한 민간요법이나 산란계(닭)에게 먹이기 위해 개발된 산란제용 동물의약품 사용에 한정되어 있는 실정이다.However, the method used by domestic beekeeping farms is limited to folk remedies using herbal medicinal herbs or animal medicines for spawning products developed to feed laying hens (chicken).
상기와 같은 문제를 해결하기 위해 본 발명은 해조 복합추출물, 개똥쑥 추출물, 및 프로폴리스 추출물을 유효성분으로 포함하는 사양액용 조성물을 제조하여 꿀벌의 면역력을 향상시킴을 목적으로 한다.In order to solve the above problems, the present invention aims to improve the immunity of bees by preparing a composition for feeding liquid containing seaweed complex extract, wormwood extract, and propolis extract as active ingredients.
또한, 본 발명은 식물 추출물에서 유래한 친환경적인 조성물을 통해 꿀벌의 개체수와 여왕벌의 산란수를 늘려 양봉농가의 생산성을 증대시킴을 목적으로 한다.In addition, an object of the present invention is to increase the productivity of beekeepers by increasing the number of bees and the number of spawning of queen bees through an eco-friendly composition derived from plant extracts.
상기 목적을 달성하기 위하여 본 발명은 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물, 구연산, L-글루타민산나트륨, 및 식물성 유지를 포함하며, 상기 해조 복합추출물, 개똥쑥 추출물, 및 프로폴리스 추출물은 40~60 ℃에서 4~8 시간 동안 열수추출되는 것을 특징으로 하는 꿀벌 사양액용 조성물을 제공한다. In order to achieve the above object, the present invention comprises a seaweed complex extract, a wormwood extract, a propolis extract, citric acid, sodium L-glutamate, and vegetable oils, and the seaweed complex extract, a wormwood extract, and a propolis extract are 40 It provides a composition for bee feeding liquid, characterized in that hot water extraction for 4 to 8 hours at ~60 ℃.
상기 사양액용 조성물은 사양액에 1/100~1/1000로 희석하여 사용될 수 있다. The composition for the feeding solution may be used by diluting 1/100 to 1/1000 in the feeding solution.
상기 해조 복합추출물은 전체 조성물 대비 0.1~5 중량%로 포함될 수 있다.The seaweed complex extract may be included in an amount of 0.1 to 5% by weight based on the total composition.
상기 개똥쑥 추출물은 전체 조성물 대비 0.1~0.5 중량%로 포함될 수 있다.The wormwood extract may be included in an amount of 0.1 to 0.5% by weight based on the total composition.
상기 프로폴리스 추출물은 전체 조성물 대비 0.1~1 중량%로 포함될 수 있다.The propolis extract may be included in an amount of 0.1 to 1% by weight based on the total composition.
전체 조성물 대비 상기 구연산은 0.5~3 중량%, 상기 L-글루타민산나트륨은 0.1~1 중량%, 및 상기 식물성유지는 0.05~1.5 중량%로 포함될 수 있다.The citric acid may be included in an amount of 0.5 to 3% by weight, the sodium L-glutamate in an amount of 0.1 to 1% by weight, and the vegetable oil in an amount of 0.05 to 1.5% by weight, based on the total composition.
또한 본 발명은 (a) 미역, 파래, 및 김을 혼합하고 40~60 ℃에서 4~8 시간 동안 열수추출하여 해조 복합추출물을 제조하는 단계; (b) 개똥쑥을 40~60 ℃에서 4~8 시간 동안 열수추출하여 개똥쑥 추출물을 제조하는 단계; (c) 프로폴리스를 40~60 ℃에서 4~8 시간 동안 열수추출하여 프로폴리스 추출물을 제조하는 단계; 및 (d) 상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물의 혼합추출물에 구연산, L-글루타민산나트륨, 및 식물성 유지를 첨가하여 사양액용 조성물을 제조하는 단계;를 포함하는 꿀벌 사양액용 조성물의 제조방법을 제공한다.In addition, the present invention comprises the steps of (a) mixing seaweed, green laver, and laver and extracting hot water at 40 to 60° C. for 4 to 8 hours to prepare a seaweed complex extract; (b) preparing a wormwood extract by hot water extraction at 40 to 60 ℃ for 4 to 8 hours; (c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And (d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, wormwood extract, and propolis extract. Provides a way.
상기 (a) 단계에서 상기 해조 복합추출물은 미역, 파래, 김과 물을 1:10~100의 중량비로 혼합하여 추출될 수 있다.In the step (a), the seaweed complex extract may be extracted by mixing seaweed, green laver, laver and water in a weight ratio of 1:10 to 100.
상기 (a) 단계에서, 상기 미역, 파래, 및 김을 1:1:1 또는 2:1:1 또는 1:2:1 또는 1:1:2의 중량비로 혼합할 수 있다.In the step (a), the seaweed, green laver, and seaweed may be mixed in a weight ratio of 1:1:1 or 2:1:1 or 1:2:1 or 1:1:2.
상기 (b) 단계에서 개똥쑥을 물과 1:10~100의 중량비로 혼합하고, 상기 (c) 단계에서 프로폴리스를 물과 1:10~100 중량비로 혼합할 수 있다.In step (b), the wormwood may be mixed with water in a weight ratio of 1:10 to 100, and in step (c), propolis may be mixed with water in a weight ratio of 1:10 to 100.
본 발명에 따른 사양액용 조성물은 식물 추출물에서 유래한 천연물을 이용하여 친환경적으로 꿀벌의 면역력을 증진시킬 수 있는 장점이 있다.The composition for feeding liquid according to the present invention has the advantage of improving the immunity of bees environmentally by using natural products derived from plant extracts.
또한, 본 발명에 따른 사양액용 조성물을 사용하면 꿀벌의 면역력 증가를 통해 꿀벌의 개체수와 산란수 증가로 양봉 산물의 생산성을 향상시킬 수 있다.In addition, when the composition for feeding liquid according to the present invention is used, the productivity of beekeeping products can be improved by increasing the number of bees and the number of spawning by increasing the immunity of bees.
도 1은 실시예 1, 실시예 2, 및 비교예 1의 사양액용 조성물을 꿀벌에 급이한 후의 온도변화를 나타낸 그래프이다.1 is a graph showing the temperature change after feeding the composition for feeding liquid of Examples 1, 2, and Comparative Example 1 to bees.
도 2는 실시예 1, 실시예 2, 및 비교예 1의 사양액용 조성물을 꿀벌에 급이한 후의 개체수 변화를 시간에 따라 나타낸 그래프이다. 2 is a graph showing changes in the number of individuals after feeding the composition for feeding liquid of Examples 1, 2, and Comparative Example 1 to bees over time.
도 3은 실시예 1, 실시예 2, 및 비교예 1의 사양액용 조성물이 여왕벌에게 전달된 후의 산란수 변화를 시간에 따라 나타낸 그래프이다. 3 is a graph showing changes in the number of spawning over time after the composition for feeding solutions of Examples 1, 2, and Comparative Example 1 is delivered to a queen bee.
본 발명은 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물, 구연산, L-글루타민산나트륨, 및 식물성 유지를 포함하며, 상기 해조 복합추출물, 개똥쑥 추출물, 및 프로폴리스 추출물은 40~60 ℃에서 4~8 시간 동안 열수추출되는 것을 특징으로 하는 꿀벌 사양액용 조성물을 제공한다. The present invention comprises seaweed complex extract, wormwood extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oils, and the seaweed complex extract, wormwood extract, and propolis extract are at 40 to 60°C. It provides a composition for bee feeding liquid, characterized in that hot water extraction for 8 hours.
또한, 본 발명은 (a) 미역, 파래, 및 김을 혼합하고 40~60 ℃에서 4~8 시간 동안 열수추출하여 해조 복합추출물을 제조하는 단계; (b) 개똥쑥을 40~60 ℃에서 4~8 시간 동안 열수추출하여 개똥쑥 추출물을 제조하는 단계; (c) 프로폴리스를 40~60 ℃에서 4~8 시간 동안 열수추출하여 프로폴리스 추출물을 제조하는 단계; 및 (d) 상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물의 혼합추출물에 구연산, L-글루타민산나트륨, 및 식물성 유지를 첨가하여 사양액용 조성물을 제조하는 단계;를 포함하는 꿀벌 사양액용 조성물의 제조방법을 제공한다.In addition, the present invention comprises the steps of (a) mixing seaweed, green laver, and laver and extracting hot water at 40 to 60° C. for 4 to 8 hours to prepare a seaweed complex extract; (b) preparing a wormwood extract by hot water extraction at 40 to 60 ℃ for 4 to 8 hours; (c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And (d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, wormwood extract, and propolis extract. Provides a way.
이하, 본원의 발명이 속하는 기술분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 본 발명의 실시형태를 들어 상세히 설명한다. 본 발명의 실시형태는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 더욱 완전하게 설명하기 위해서 제공되는 것이다. 따라서, 본 발명의 실시형태는 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 이하 설명하는 실시형태로 한정되는 것은 아니다.Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily implement the present invention. Embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art. Accordingly, embodiments of the present invention may be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below.
하기의 설명에서는 본 발명의 실시예를 이해하는데 필요한 부분만이 설명되며, 그 이외 부분의 설명은 본 발명의 요지를 흩트리지 않는 범위에서 생략될 것이라는 것을 유의하여야 한다.In the following description, it should be noted that only parts necessary for understanding the embodiments of the present invention will be described, and descriptions of other parts will be omitted without distracting the gist of the present invention.
이하에서 설명되는 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념으로 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다. 따라서 본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 바람직한 실시예에 불과할 뿐이고, 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형예들이 있을 수 있음을 이해하여야 한다.Terms or words used in the present specification and claims described below should not be construed as being limited to a conventional or dictionary meaning, and the inventor is appropriate as a concept of terms in order to describe his own invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention on the basis of the principle that it can be defined as such. Therefore, the embodiments described in the present specification and the configurations shown in the drawings are only preferred embodiments of the present invention, and do not represent all the technical spirit of the present invention, and various equivalents that can replace them at the time of application It should be understood that there may be variations and variations.
이하, 본 발명의 일 실시예에 따른 꿀벌의 면역력 증가를 위한 사양액용 조성물 및 그의 제조방법을 구체적으로 설명한다.Hereinafter, a composition for feeding liquid for increasing the immunity of bees according to an embodiment of the present invention and a method of manufacturing the same will be described in detail.
본 발명은 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물, 구연산, L-글루타민산나트륨, 및 식물성 유지를 포함하며, 상기 해조 복합추출물, 개똥쑥 추출물, 및 프로폴리스 추출물은 40~60 ℃에서 4~8 시간 동안 열수추출되는 것을 특징으로 하는 꿀벌 사양액용 조성물을 제공한다.The present invention comprises seaweed complex extract, wormwood extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oils, and the seaweed complex extract, wormwood extract, and propolis extract are at 40 to 60°C. It provides a composition for bee feeding liquid, characterized in that hot water extraction for 8 hours.
해조류(seaweed)는 해양에서 서식하는 거대조류(macroalgae)로서 바다에 생육하는 다세포 원생생물을 지칭하며 육안으로 관찰할 수 있는 크기를 가진 점에서 단세포의 미세조류(microalgae)와 구별된다. 해조류 내의 구분은 광합성 색소에 따라 녹조류(green seaweed), 갈조류(brown seaweed), 홍조류(red seaweed)로 나눠지며 각각이 지니는 서식환경 및 구성 성분에서도 차이를 보인다.Seaweed is a macroalgae that lives in the ocean and refers to a multicellular protozoa that grows in the sea, and is distinguished from single-celled microalgae in that it has a size that can be observed with the naked eye. The classification within seaweed is divided into green seaweed, brown seaweed, and red seaweed according to the photosynthetic pigment, and there are differences in their habitat environment and composition.
상기 녹조류는 파래, 청각, 청태 등을 포함하며, 해양 또는 담수에서 생육된다. 상기 홍조류는 우뭇가사리, 김, 카라니긴 등을 포함하며, 해양에서 생육한다. The green algae include green, auditory, blue, and the like, and are grown in the ocean or fresh water. The red algae include agar, laver, karanigin, and the like, and grow in the ocean.
갈조류는 톳, 미역, 다시마, 대황, 모자반 등이 있으며, 상기 갈조류는 특히 건물기준으로 탄수화물을 약 30~67 %까지 함유하는 특징을 보인다. 갈조류가 함유하는 다당류는 대부분이 알긴산으로 구성되어 있다. 알긴산은 갈조류의 세포막 또는 세포간 물질을 구성하는 주성분으로 β-D-mannuronic acid(M)와 α-L-guluronic acid(G)가 α-1, 4결합 또는 β-1,4 결합한 hetero형 다당류로 알려져 있다. Brown algae include tot, seaweed, kelp, rhubarb, and mackerel, and the brown algae particularly contain up to about 30-67% carbohydrates on a dry matter basis. The polysaccharides contained in brown algae are mostly composed of alginic acid. Alginic acid is a hetero-type polysaccharide in which β-D-mannuronic acid (M) and α-L-guluronic acid (G) are α-1, 4 bonded or β-1,4 bonded as the main component of the cell membrane or intercellular material of brown algae. Known as.
본 발명의 일 실시예에 따른 해조 복합추출물에는 유효성분으로 알긴산이 포함되어 있다. 알긴산에 의해 산화질소 생산 및 과립성 백혈구의 확산이 강화되므로 꿀벌의 면역력을 향상시킬 수 있다. The seaweed complex extract according to an embodiment of the present invention contains alginic acid as an active ingredient. Alginic acid enhances the production of nitric oxide and the spread of granular leukocytes, so it can improve the immunity of bees.
상기 알긴산은 해조 복합추출물 대비 7.5~500 mg/kg로 함유될 수 있으며, 상기 범위를 벗어나는 경우 알긴산으로 인한 꿀벌의 면역력 증진효과가 미미하므로 상기 범위로 포함되는 것이 바람직하다. The alginic acid may be contained in an amount of 7.5 to 500 mg/kg compared to the seaweed complex extract, and if it is out of the above range, the effect of enhancing the immunity of bees due to alginic acid is insignificant, so it is preferable to be included in the above range.
본 발명의 일 실시예에 따르면 상기 해조 복합추출물은 미역, 파래, 및 김을 혼합하여 제조될 수 있으며, 상기 미역, 파래, 및 김을 1:1:1 또는 2:1:1 또는 1:2:1 또는 1:1:2의 중량비로 혼합하여 제조되는 것이 바람직하다. 상기 중량비로 혼합하여야 알긴산이 유효한 범위 내에서 추출가능하므로 상기 중량비로 혼합하는 것이 바람직하다. According to an embodiment of the present invention, the seaweed complex extract may be prepared by mixing seaweed, green seaweed, and seaweed, and the seaweed, green seaweed, and seaweed 1:1:1 or 2:1:1 or 1:2 It is preferably prepared by mixing in a weight ratio of :1 or 1:1:2. Since alginic acid can be extracted within an effective range only when it is mixed in the weight ratio, it is preferable to mix it in the weight ratio.
상기 추출물을 추출하는 추출용매로는 물, 탄소수 1 내지 4급의 저급알코올, 또는 이들의 혼합용매를 사용할 수 있다. 혼합용매는 특별히 한정되는 것은 아니지만, 물을 사용하는 것이 바람직하다.As an extraction solvent for extracting the extract, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but it is preferable to use water.
상기 해조 복합추출물은 미역, 파래, 김을 물과 1:10 내지 100의 중량비로 혼합하여 40~60 ℃에서 4~8 시간 동안 추출하여 제조될 수 있다. 상기 미역, 파래, 김과 물의 중량비가 상기 범위를 벗어나는 경우 추출물의 유효성분이 적은 양으로 추출될 수 있다. 또한, 추출온도가 40 ℃ 미만인 경우에는 유효성분이 추출되지 않을 수 있으며, 60 ℃를 초과하는 경우에는 불필요한 물질의 추출로 인해 유효성분이 적은 양으로 추출될 수 있다.The seaweed complex extract may be prepared by mixing seaweed, green laver, and seaweed in a weight ratio of 1:10 to 100 with water and extracting for 4 to 8 hours at 40 to 60°C. When the weight ratio of the seaweed, green laver, laver and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
본 발명에 있어서, 다시마(Laminaria japonica)는 갈조식물문(Phaeophyta) 갈조강(Phaeophyceae) 다시마목(Laminariales) 다시마과(Laminariaceae)에 속하는 해조류로, 외관상 엽상부, 줄기, 뿌리로 구분되며, 엽상부는 띠모양으로 길고, 엽상부의 중앙부분은 두터운 중대부(中帶部)를 이루며, 중대부의 양 가는 세로로 흠이 있다. 줄기는 보통 원주상이며 직립하며, 뿌리는 줄기의 하부에서 차상으로 분지하는 수지상의 형태를 나타낸다. 상기 다시마는 예로부터 우리나라를 비롯한 일본, 중국에서 식용으로 널리 사용되어 왔다.In the present invention, the kelp (Laminaria japonica) is a seaweed belonging to the Phaeophyta, Phaeophyceae) Kelp tree (Laminariales) Kelp family (Laminariaceae), and is divided into upper leaves, stems, and roots in appearance, and the upper leaves are bands. It is long in shape, and the central part of the upper part of the leaf forms a thick middle part, and both sides of the middle part have vertical flaws. The stem is usually columnar and erect, and the root has a dendritic form branching from the lower part of the stem to the second phase. Since ancient times, the kelp has been widely used for food in Japan, China, including Korea.
또한 본 발명에 있어서, 미역(Undaria pinnatifida)은 갈조식물문(Phaeophyta) 갈조강(Phaeophyceae) 다시마목(Laminariales) 미역과(Alariaceae)에 속하는 해조류로, 기부는 수지상이고, 줄기는 편원이며, 그 상부는 엽상부의 중륵으로 되고 잎은 자우에 우상열편을 가진다. 우리나라와 일본에 분포하며, 우리나라에서는 서해안의 백령도에서 동해안 끝에 이르기까지 넓은 영역에 분포하며, 부산, 완도 등 남해안 일대에서 양식하고, 우리나라와 일본에서 예로부터 식용되어왔다.In addition, in the present invention, the seaweed (Undaria pinnatifida) is a seaweed belonging to the algae family of Phaeophyta, Phaeophyceae) Kelp tree (Laminariales) Seaweed family (Alariaceae), the base is dendritic, the stem is oblate, and the upper part Is the middle of the upper leaf, and the leaf has the right upper lobe on the jaw. It is distributed in Korea and Japan. In Korea, it is distributed in a wide area from Baengnyeongdo Island on the west coast to the end of the east coast. It is cultivated in the southern coastal areas such as Busan and Wando, and has been edible in Korea and Japan since ancient times.
한편, 상기 파래는 갈파래과에 속하는 해조류로, 철분, 메틸메티오닌, 식이섬유 및 클로로필 성분이 풍부하게 함유되어 있어, 빈혈개선, 니코틴 중화, 변비개선, 다이어트 및 구취제거에 효과적이다. 파래는 오염에 강하고 부영양화된 환경에서도 잘 생육하며, 육상 식물과는 달리 황산기를 함유한 다당을 다량 함유하고 있으며, 주로 글루쿠로노-라암옥실로글리칸(glucurono-rhamnoxyloglycan)으로 구성되어 있고, 황산기와 우론산이 각각 약 20 %와 21 %정도 함유되어 있다. On the other hand, the green onion is a seaweed belonging to the Galparaeaceae family, and it is rich in iron, methylmethionine, dietary fiber and chlorophyll components, and is effective in improving anemia, neutralizing nicotine, improving constipation, dieting and removing bad breath. Green lava is resistant to pollution and grows well in eutrophied environments, and unlike land plants, it contains a large amount of polysaccharide containing a sulfuric acid group, and is mainly composed of glucurono-rhamnoxyloglycan. It contains about 20% and 21% of sulfuric acid and uronic acid, respectively.
본 발명의 일 실시예에 따른 해조 복합추출물은 전체 사양액용 조성물 대비 0.1~5 중량%로 포함될 수 있다.The seaweed complex extract according to an embodiment of the present invention may be included in an amount of 0.1 to 5% by weight relative to the composition for the total specification solution.
개똥쑥(Artemisia annua)이란 잔잎쑥, 개땅쑥, 또는 청호쑥으로 불리우는 초롱꽃목 국화과의 한해살이 풀을 말한다. 길가나 빈터, 강가에서 약 1m 가량으로 자라며, 쑥 중에서 잎의 뒷면이 푸른색을 띄기 때문에 청호라 불리운다. 한방에서는 발열 감기, 학질, 소아경기, 소화불량 및 이질 등의 치료에 효과가 있는 것으로 알려져 있다.Artemisia annua refers to the annual herb of the Chrysanthemum family of the Chrysanthemum family, which is called fine leaf mugwort, dog mugwort, or blue mugwort. It grows to about 1m in roadsides, vacant lands, and riversides, and is called Cheongho because the back side of the leaf is blue among mugwort. In oriental medicine, it is known to be effective in treating fever, cold, dysentery, childhood, indigestion and dysentery.
상기 개똥쑥에는 항산화, 항염, 항암, 면역조절 효과가 있는 폴리페놀(polyphenol) 화합물인 아르테미시닌(Artemisinin) 성분이 함유되어 있다. 특히 항산화 활성은 가장 우수한 소재로 알려진 오레가노(oregano)의 50~70 %를 함유하는 것으로 알려져 있다. Artemisinin, a polyphenol compound that has antioxidant, anti-inflammatory, anti-cancer, and immunomodulatory effects, is contained in the wormwood. In particular, it is known to contain 50-70% of oregano, which is known as the most excellent material for its antioxidant activity.
본 발명의 일 실시예에 따른 개똥쑥 추출물에는 유효성분으로 폴리페놀이 포함되어 있으며, 상기 폴리페놀은 개똥쑥 추출물 대비 0.01~1000 mg/kg로 함유될 수 있다. 상기 폴리페놀은 항산화 효과, 항균, 및 항생효과가 있고 스트레스 완화에 도움울 주어 벌꿀의 면역력을 증진시킬 수 있다. In the embodiment of the present invention, a polyphenol is included as an active ingredient, and the polyphenol may be contained in an amount of 0.01 to 1000 mg/kg compared to the wormwood extract. The polyphenol has antioxidant, antibacterial, and antibiotic effects, and helps relieve stress, thereby enhancing the immunity of honey.
상기 추출물을 추출하는 추출용매로는 물, 탄소수 1 내지 4급의 저급알코올, 또는 이들의 혼합용매를 사용할 수 있다. 혼합용매는 특별히 한정되는 것은 아니지만, 물을 사용하는 것이 바람직하다.As an extraction solvent for extracting the extract, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but it is preferable to use water.
상기 개똥쑥 추출물은 개똥쑥을 물과 1:10 내지 100의 중량비로 혼합하여 40~60 ℃에서 4~8 시간 동안 추출하여 제조될 수 있다. 상기 개똥쑥과 물의 중량비가 상기 범위를 벗어나는 경우 추출물의 유효성분이 적은 양으로 추출될 수 있다. 또한, 추출온도가 40 ℃ 미만인 경우에는 유효성분이 추출되지 않을 수 있으며, 60 ℃를 초과하는 경우에는 불필요한 물질의 추출로 인해 유효성분이 적은 양으로 추출될 수 있다.The wormwood extract may be prepared by mixing the wormwood with water in a weight ratio of 1:10 to 100 and extracting for 4 to 8 hours at 40 to 60°C. When the weight ratio of the wormwood and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
본 발명의 일 실시예에 따른 개똥쑥 추출물은 전체 사양액용 조성물 대비 0.1~0.5 중량%로 포함될 수 있다.The wormwood extract according to an embodiment of the present invention may be included in an amount of 0.1 to 0.5% by weight relative to the composition for the total specification solution.
프로폴리스(propolis)는 꿀벌이 생존과 번식을 위해 여러 식물에서 뽑아낸 수지와 같은 물질에 침과 효소 등을 섞어서 만든 물질로서, 꿀벌이 벌집에 저장하는 수지상 물질에 수지, 밀랍, 정유, 화분, 플라보노이드 등이 함유되어 있다.Propolis is a material made by mixing saliva and enzymes with resin-like materials extracted from various plants by bees for survival and reproduction. It contains flavonoids and the like.
프로폴리스는 천연 항생물질로서 유기물과 미네랄(무기염류)이 가장 많고 이와 함께 104종 정도의 성분이 들어있는 것으로 공지되어 있으며, 그 주요한 유효성분으로서 플라보노이드, 폴리페놀, 방향족 카르복실산, 방향족 알데히드 등이 알려져 있고 주요 작용은 항균 작용, 항산화 작용, 항염증 작용, 국소마취 작용, 비루스 증식 억제작용, 면역 조절작용 등이 있다.Propolis is a natural antibiotic and is known to contain the most organic matters and minerals (inorganic salts), along with 104 kinds of ingredients. As its main active ingredients, flavonoids, polyphenols, aromatic carboxylic acids, aromatic aldehydes, etc. It is known and its main actions include antibacterial action, antioxidant action, anti-inflammatory action, local anesthetic action, viral proliferation inhibitory action, and immune regulation action.
본 발명의 일 실시예에 따른 프로폴리스 추출물은 유효성분으로서 폴리페놀을 포함할 수 있으며, 상기 폴리페놀은 프로폴리스 추출물 대비 0.001~1000 mg/kg로 함유될 수 있다. 상기 폴리페놀은 항산화 효과, 항균, 및 항생효과가 있고 스트레스 완화에 도움울 주어 벌꿀의 면역력을 증진시킬 수 있다. The propolis extract according to an embodiment of the present invention may contain polyphenol as an active ingredient, and the polyphenol may be contained in an amount of 0.001 to 1000 mg/kg compared to the propolis extract. The polyphenol has antioxidant, antibacterial, and antibiotic effects, and helps relieve stress, thereby enhancing the immunity of honey.
상기 추출물을 추출하는 추출용매로는 물, 탄소수 1 내지 4급의 저급알코올, 또는 이들의 혼합용매를 사용할 수 있다. 혼합용매는 특별히 한정되는 것은 아니지만, 물을 사용하는 것이 바람직하다.As an extraction solvent for extracting the extract, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but it is preferable to use water.
상기 프로폴리스 추출물은 프로폴리스를 물과 1:10 내지 100의 중량비로 혼합하여 40~60 ℃에서 추출하여 제조될 수 있다. 상기 프로폴리스와 물의 중량비가 상기 범위를 벗어나는 경우 추출물의 유효성분이 적은 양으로 추출될 수 있다. 또한, 추출온도가 40 ℃ 미만인 경우에는 유효성분이 추출되지 않을 수 있으며, 60 ℃를 초과하는 경우에는 불필요한 물질의 추출로 인해 유효성분이 적은 양으로 추출될 수 있다.The propolis extract may be prepared by mixing propolis with water in a weight ratio of 1:10 to 100 and extracting at 40 to 60°C. When the weight ratio of the propolis and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
본 발명의 일 실시예에 따른 프로폴리스 추출물은 전체 조성물 대비 0.1~1 중량%로 포함될 수 있다. The propolis extract according to an embodiment of the present invention may be included in an amount of 0.1 to 1% by weight based on the total composition.
상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물의 혼합물은 전체 사양액용 조성물 대비 0.3~6.5 중량%로 함유될 수 있다. 상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물의 함량이 전체 조성물 대비 0.3 중량% 미만인 경우 벌꿀의 면역력 증진에 있어 원하는 효과를 내기 어려우며, 함량이 6.5 중량%를 초과하는 경우는 원료의 투입대비 효과의 향상이 미미하므로 경제적으로 바람직하지 못하다. The mixture of the seaweed complex extract, wormwood extract, and propolis extract may be contained in an amount of 0.3 to 6.5% by weight compared to the composition for the total specification solution. When the content of the seaweed complex extract, wormwood extract, and propolis extract is less than 0.3% by weight of the total composition, it is difficult to produce the desired effect in enhancing the immunity of honey, and when the content exceeds 6.5% by weight, it is effective compared to the input of the raw material. It is not economically desirable because the improvement of is minimal.
본 발명에 따른 사양액용 조성물은 상기 유효성분 외에 구연산, L-글루타민산나트륨, 식물성 유지를 더 포함할 수 있다. The composition for feeding liquid according to the present invention may further include citric acid, sodium L-glutamate, and vegetable fats in addition to the active ingredients.
상기 구연산은 전체 조성물 대비 0.5~3 중량%로 포함될 수 있고, 상기 L-글루타민산나트륨은 0.1~1 중량%로 포함될 수 있으며, 상기 식물성 유지는 0.05~1.5 중량%로 포함될 수 있다. The citric acid may be included in an amount of 0.5 to 3% by weight of the total composition, the sodium L-glutamate may be included in an amount of 0.1 to 1% by weight, and the vegetable oil may be included in an amount of 0.05 to 1.5% by weight.
상기 사양액용 조성물은 사양액에 1/100~1/1000로 희석하여 사용될 수 있다. 상기 사양액용 조성물은 용도에 따라 희석 농도를 다르게 하여 사용할 수 있다. 예를 들어 강군을 만드는 용도 및 월동 사양으로 사용하는 경우 사양액 100 ℓ에 사양액용 조성물 500 ㎖를 혼합하여 1/200로 희석하여 사용하는 것이 바람직하다.The composition for the feeding solution may be used by diluting 1/100 to 1/1000 in the feeding solution. The composition for feeding liquid may be used with different dilution concentrations depending on the application. For example, when it is used for making a strong group or for wintering, it is preferable to mix 500 ml of the non-freezing composition with 100 ℓ of the non-freezing solution, and dilute it to 1/200.
또한, 진드기 저항성을 증가시키는 용도로 사용하는 경우 사양액 100 ℓ에 사양액용 조성물 250 ㎖를 혼합하여 1/400 로 희석하여 사용하는 것이 바람직하다.In addition, in the case of use for increasing tick resistance, it is preferable to use after mixing 250 ml of the composition for non-watering solution to 100 ℓ of the non-watering solution and diluting it to 1/400.
또한, 일반사양 용도로 사용하는 경우 사양액 100 ℓ에 사양액용 조성물 333 ㎖를 혼합하여 1/300 로 희석하여 사용하는 것이 바람직하다.In addition, in the case of using for general specification purposes, it is preferable to use after mixing 333 ml of the composition for non-standard solution with 100 ℓ of the non-standard solution and diluting it to 1/300.
본 발명의 다른 측면에 의하면, 본 발명은 (a) 미역, 파래, 및 김을 혼합하고 40~60 ℃에서 4~8 시간 동안 열수추출하여 해조 복합추출물을 제조하는 단계; (b) 개똥쑥을 40~60 ℃에서 4~8 시간 동안 열수추출하여 개똥쑥 추출물을 제조하는 단계; (c) 프로폴리스를 40~60 ℃에서 4~8 시간 동안 열수추출하여 프로폴리스 추출물을 제조하는 단계; 및 (d) 상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물의 혼합추출물에 구연산, L-글루타민산나트륨, 및 식물성 유지를 첨가하여 사양액용 조성물을 제조하는 단계;를 포함하는 꿀벌 사양액용 조성물의 제조방법을 제공한다.According to another aspect of the present invention, the present invention comprises the steps of: (a) mixing seaweed, green laver, and laver and extracting hot water at 40 to 60° C. for 4 to 8 hours to prepare a seaweed complex extract; (b) preparing a wormwood extract by hot water extraction at 40 to 60 ℃ for 4 to 8 hours; (c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And (d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, wormwood extract, and propolis extract. Provides a way.
상기 (a) 단계에서 해조 복합추출물은 미역, 파래, 및 김을 혼합하여 제조될 수 있으며, 상기 미역, 파래, 및 김을 1:1:1 또는 2:1:1 또는 1:2:1 또는 1:1:2의 중량비로 혼합하여 제조되는 것이 바람직하다. In the step (a), the seaweed complex extract may be prepared by mixing seaweed, green laver, and laver, and the seaweed, green laver, and laver 1:1:1 or 2:1:1 or 1:2:1 or It is preferably prepared by mixing in a weight ratio of 1:1:2.
상기 (a) 단계에서 상기 추출물을 추출하는 추출용매로는 물, 탄소수 1 내지 4급의 저급알코올, 또는 이들의 혼합용매를 사용할 수 있다. 혼합용매는 특별히 한정되는 것은 아니지만, 물을 사용하는 것이 바람직하다.As the extraction solvent for extracting the extract in step (a), water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but it is preferable to use water.
상기 해조 복합추출물은 미역, 파래, 김을 물과 1:10 내지 100의 중량비로 혼합하여 40~60 ℃에서 4~8 시간 동안 추출하여 제조될 수 있다. 상기 미역, 파래, 김과 물의 중량비가 상기 범위를 벗어나는 경우 추출물의 유효성분이 적은 양으로 추출될 수 있다. 또한, 추출온도가 40 ℃ 미만인 경우에는 유효성분이 추출되지 않을 수 있으며, 60 ℃를 초과하는 경우에는 불필요한 물질의 추출로 인해 유효성분이 적은 양으로 추출될 수 있다.The seaweed complex extract may be prepared by mixing seaweed, green laver, and seaweed in a weight ratio of 1:10 to 100 with water and extracting for 4 to 8 hours at 40 to 60°C. When the weight ratio of the seaweed, green laver, laver and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
상기 (b) 단계에서 상기 추출물을 추출하는 추출용매로는 물, 탄소수 1 내지 4급의 저급알코올, 또는 이들의 혼합용매를 사용할 수 있다. 혼합용매는 특별히 한정되는 것은 아니지만, 물을 사용하는 것이 바람직하다.As the extraction solvent for extracting the extract in step (b), water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but it is preferable to use water.
상기 개똥쑥 추출물은 개똥쑥을 물과 1:10 내지 100의 중량비로 혼합하여 40~60 ℃에서 4~8 시간 동안 추출하여 제조될 수 있다. 상기 개똥쑥과 물의 중량비가 상기 범위를 벗어나는 경우 추출물의 유효성분이 적은 양으로 추출될 수 있다. 또한, 추출온도가 40 ℃ 미만인 경우에는 유효성분이 추출되지 않을 수 있으며, 60 ℃를 초과하는 경우에는 불필요한 물질의 추출로 인해 유효성분이 적은 양으로 추출될 수 있다.The wormwood extract may be prepared by mixing the wormwood with water in a weight ratio of 1:10 to 100 and extracting for 4 to 8 hours at 40 to 60°C. When the weight ratio of the wormwood and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
상기 (c) 단계에서 상기 추출물을 추출하는 추출용매로는 물, 탄소수 1 내지 4급의 저급알코올, 또는 이들의 혼합용매를 사용할 수 있다. 혼합용매는 특별히 한정되는 것은 아니지만, 물을 사용하는 것이 바람직하다.As the extraction solvent for extracting the extract in step (c), water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but it is preferable to use water.
상기 프로폴리스 추출물은 프로폴리스를 물과 1:10 내지 100의 중량비로 혼합하여 40~60 ℃에서 4~8 시간 동안 추출하여 제조될 수 있다. 상기 프로폴리스와 물의 중량비가 상기 범위를 벗어나는 경우 추출물의 유효성분이 적은 양으로 추출될 수 있다. 또한, 추출온도가 40 ℃ 미만인 경우에는 유효성분이 추출되지 않을 수 있으며, 60 ℃를 초과하는 경우에는 불필요한 물질의 추출로 인해 유효성분이 적은 양으로 추출될 수 있다.The propolis extract may be prepared by mixing propolis with water in a weight ratio of 1:10 to 100 and extracting for 4 to 8 hours at 40 to 60°C. When the weight ratio of the propolis and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, when the extraction temperature is less than 40° C., the active ingredient may not be extracted, and when the extraction temperature exceeds 60° C., the active ingredient may be extracted in a small amount due to the extraction of unnecessary substances.
상기 (d) 단계에서 구연산, L-글루타민산나트륨, 및 식물성 유지를 더 첨가하여 사양액용 조성물을 제조한다. 상기 구연산은 전체 조성물 대비 0.5~3 중량%로 첨가될 수 있고, 상기 L-글루타민산나트륨은 0.1~1 중량%로 첨가될 수 있으며, 상기 식물성 유지는 0.05~1.5 중량%로 첨가될 수 있다.In the step (d), citric acid, sodium L-glutamate, and vegetable fats and oils are further added to prepare a composition for feeding liquid. The citric acid may be added in an amount of 0.5 to 3% by weight relative to the total composition, the sodium L-glutamate may be added in an amount of 0.1 to 1% by weight, and the vegetable oil may be added in an amount of 0.05 to 1.5% by weight.
상기 단계에 의해 제조된 사양액용 조성물은 사양액에 1/100~1/1000로 희석하여 사용할 수 있다. 상기 사양액용 조성물은 용도에 따라 희석 농도를 다르게 하여 사용할 수 있다. 예를 들어 강군을 만드는 용도 및 월동사양으로 사용하는 경우 사양액 100 ℓ에 사양액용 조성물 500 ㎖를 혼합하여 1/200로 희석하여 사용하는 것이 바람직하다.The composition for a feeding solution prepared by the above step can be used by diluting 1/100 to 1/1000 in a feeding solution. The composition for feeding liquid may be used with different dilution concentrations depending on the application. For example, in the case of making a strong group and using it for wintering specifications, it is preferable to mix 500 ml of the non-freezing composition with 100 ℓ of the non-freezing solution, and dilute it to 1/200.
또한, 진드기 저항성을 증가시키는 용도로 사용하는 경우 사양액 100 ℓ에 사양액용 조성물 250 ㎖를 혼합하여 1/400 로 희석하여 사용하는 것이 바람직하다.In addition, in the case of use for increasing tick resistance, it is preferable to use after mixing 250 ml of the composition for non-watering solution to 100 ℓ of the non-watering solution and diluting it to 1/400.
또한, 일반사양 용도로 사용하는 경우 사양액 100ℓ 에 사양액용 조성물 333 ㎖를 혼합하여 1/300 로 희석하여 사용하는 것이 바람직하다.In addition, in the case of using for general specification purposes, it is preferable to mix 333 ml of the composition for non-existence solution with 100ℓ of the non-exchange solution, and dilute it to 1/300.
본 발명에 따른 사양액용 조성물은 식물 추출물에서 유래한 천연물을 이용하여 친환경적으로 꿀벌의 면역력을 증진시킬 수 있는 장점이 있다.The composition for feeding liquid according to the present invention has the advantage of improving the immunity of bees environmentally by using natural products derived from plant extracts.
또한, 본 발명에 따른 사양액용 조성물을 사용하면 꿀벌의 면역력 증가를 통해 꿀벌의 개체수와 산란수 증가로 양봉 산물의 생산성을 향상시킬 수 있다.In addition, when the composition for feeding liquid according to the present invention is used, the productivity of beekeeping products can be improved by increasing the number of bees and the number of spawning by increasing the immunity of bees.
실시예Example
이하, 본 발명을 구체적으로 설명하기 위해 실시예를 들어 상세하게 설명하기로 한다. 그러나, 본 발명에 따른 실시예는 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 아래에서 상술하는 실시예에 한정되는 것으로 해석되어서는 안 된다. 본 발명의 실시예는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해서 제공되는 것이다.Hereinafter, examples will be described in detail in order to describe the present invention in detail. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely describe the present invention to those of ordinary skill in the art.
<비교예 1><Comparative Example 1>
30 % 설탕물을 제조하여 사양액으로 사용하였다.30% sugar water was prepared and used as a feeding solution.
<실시예 1><Example 1>
먼저 해조 복합추출물을 제조하기 위하여 국내산 미역, 파래, 김을 모두 생물로 구매하였다. 상기 미역, 파래, 김의 염분 및 이물질의 제거를 위해 흐르는 물로 3회 이상 수세 후 일차 냉동하여 동결건조를 실시하였다. 이후, 미역, 파래, 김의 중량비가 1:1:1이 되도록 혼합하였다. 상기 혼합물과 물이 10 %(w/w)가 되도록 혼합하고, 24 시간 동안 침지시킨 뒤 50 ℃에서 6 시간 동안 교반 추출한 다음 여과하여 추출물을 제조하였다.First, in order to manufacture the seaweed complex extract, all domestic seaweed, green laver, and seaweed were purchased as living organisms. In order to remove salt and foreign substances from the seaweed, green seaweed, and seaweed, it was washed with running water three times or more, then first frozen and freeze-dried. Thereafter, the weight ratio of seaweed, green seaweed, and seaweed was mixed to be 1:1:1. The mixture and water were mixed to be 10% (w/w), immersed for 24 hours, extracted with stirring at 50° C. for 6 hours, and filtered to prepare an extract.
다음으로 개똥쑥의 잎을 수세한 다음 개똥쑥 잎과 물이 10 %(w/w)가 되도록 혼합하여 50 ℃에서 6 시간 동안 교반 추출한 다음 여과하여 개똥쑥 추출물을 제조하였다. 또한, 프로폴리스 분말과 물을 10 %(w/w)가 되도록 혼합하고 50 ℃에서 6 시간 동안 교반 추출한 다음 여과하여 프로폴리스 추출물을 제조하였다.Next, the leaves of the wormwood were washed with water, mixed so that the leaves and water were 10% (w/w), extracted with stirring at 50° C. for 6 hours, and filtered to prepare a wormwood extract. In addition, the propolis powder and water were mixed so as to be 10% (w/w), extracted with stirring at 50° C. for 6 hours, and then filtered to prepare a propolis extract.
상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물을 혼합한 뒤 구연산, L-글루타민산나트륨, 식물성 유지를 더 첨가하여 사양액용 조성물을 제조하였다. 제조된 사양액용 조성물을 비교예 1의 30 % 설탕물에 1/200로 희석하여 첨가하였다.After mixing the seaweed complex extract, wormwood extract, and propolis extract, citric acid, sodium L-glutamate, and vegetable oil were further added to prepare a composition for feeding liquid. The prepared composition for specification solution was diluted 1/200 in 30% sugar water of Comparative Example 1 and added.
<실시예 2><Example 2>
상기 실시예 1과 동일한 방법에 의해 제조된 사양액용 조성물을 비교예 1의 30 % 설탕물에 1/250로 희석하여 첨가하였다.The composition for a feeding solution prepared by the same method as in Example 1 was diluted 1/250 in 30% sugar water of Comparative Example 1 and added.
실험예 1: 사양액 급이 후 꿀벌의 온도변화Experimental Example 1: Temperature change of bees after feeding the feeding solution
실시예 1, 2 및 비교예 1의 사양액을 오전 0시, 오전 4시, 오전 8시 3회에 걸쳐 20분 동안 꿀벌에 급이하였으며, 급이 후의 온도변화를 도 1에 나타내었다.The feeding solutions of Examples 1 and 2 and Comparative Example 1 were fed to bees for 20 minutes over three times at 0 am, 4 am, and 8 am, and the temperature change after feeding is shown in FIG. 1.
도 1을 참조하면, 아무것도 처리하지 않은 비교예 1의 사양액을 급이한 경우와 비교하여 실시예 1 및 실시예 2를 급이한 경우 온도가 0.5 내지 2 ℃ 상승한 것을 확인할 수 있었다. Referring to FIG. 1, it was confirmed that the temperature increased by 0.5 to 2° C. when feeding Examples 1 and 2 compared to the case of feeding the feeding solution of Comparative Example 1 without any treatment.
이와 같은 결과는 사양액용 조성물을 첨가하여 벌의 성장과 활동성이 증가함으로써 계상(벌집) 내의 온도가 상승한 것으로 판단된다. 온도의 상승은 봉군의 강세를 나타내는 지표로 활용되기 때문에, 본원발명에 따른 사양액용 조성물을 사용하는 경우 벌꿀의 활동성을 증가시키고 월동을 극복시킬 수 있으며, 강군을 형성할 수 있는 효과를 확인하였다.Such a result is judged to increase the temperature in the cage (honeycomb) by increasing the growth and activity of the bees by adding the composition for feeding liquid. Since the increase in temperature is used as an indicator of the strength of Bonggun, when the composition for feeding liquid according to the present invention is used, the activity of honey can be increased, wintering can be overcome, and the effect of forming a strong group was confirmed.
실험예 2: 사양액 급이 후 꿀벌의 개체수 변화Experimental Example 2: Change in the number of bees after feeding the feeding solution
실시예 1, 2 및 비교예 1의 사양액을 오전 0시, 오전 4시, 오전 8시 3회에 걸쳐 20분 동안 꿀벌에 급이하였으며, 급이 후의 개체수 변화를 도 2에 나타내었다.The feeding solutions of Examples 1 and 2 and Comparative Example 1 were fed to bees for 20 minutes over three times at 0 am, 4 am, and 8 am, and the change in the number of individuals after feeding is shown in FIG. 2.
도 2를 참조하면, 개체수 증가는 비교예 1이 7.3 %, 실시예 1이 35.1 %, 실시예 2가 15.9 %로 나타나 아무것도 처리하지 않은 사양액을 급이하는 것보다 본원발명에 따른 사양액용 조성물을 첨가하여 급이하는 것이 꿀벌의 개체수 증가에 도움이 된다는 것을 확인할 수 있었다.2, the increase in the number of individuals was 7.3% in Comparative Example 1, 35.1% in Example 1, and 15.9% in Example 2. It could be confirmed that feeding by adding honey was helpful to increase the number of bees.
또한, 아무것도 처리하지 않은 비교예 1의 사양액을 급이한 경우 15 일이 지난 후 개체수가 증가하였으나 30 일 지난 후 개체수가 다시 감소하였으며, 사양액용 조성물을 250 배로 희석하여 사용한 실시예 2의 경우도 15 일이 지난 후 오히려 개체수가 감소한 것을 확인할 수 있었다. 사양액용 조성물을 200 배로 희석하여 사용한 실시예 1의 경우 시간이 지날수록 점점 꿀벌의 개체수가 증가하는 양상을 보이며 결과적으로 개체수가 35.1 % 증가한 것으로 확인되었다. In addition, in the case of feeding the feeding solution of Comparative Example 1 without any treatment, the number of individuals increased after 15 days, but the number of individuals decreased again after 30 days, and in the case of Example 2, in which the composition for feeding solution was diluted 250 times. After 15 days passed, it could be confirmed that the number of individuals decreased. In the case of Example 1, in which the composition for feeding solution was diluted 200 times, the number of bees gradually increased as time passed, and as a result, it was confirmed that the number of bees increased by 35.1%.
따라서 본원발명에 따른 조성물을 사양액에 200 배 희석하여 사용하는 것이 가장 바람직함을 확인할 수 있었으며, 개체수 증가에 영향이 없는 계절에는 희석배수를 250 배 이상으로 희석하여 사용하는 것이 사양관리에 효과적임을 확인할 수 있었다. Therefore, it was confirmed that it is most preferable to use the composition according to the present invention by diluting 200 times in the feeding solution, and in the season when there is no effect on the increase in the number of individuals, diluting the dilution factor by more than 250 times is effective for feeding management I could confirm.
실험예 3: 사양액 급이 후 꿀벌의 산란수 변화Experimental Example 3: Change in the number of spawning bees after feeding the feeding solution
실시예 1, 2 및 비교예 1의 사양액을 오전 0시, 오전 4시, 오전 8시 3회에 걸쳐 20분 동안 급이하여 여왕벌에 전달하였으며, 급이 후의 산란수 변화를 도 3에 나타내었다. 여왕벌의 산란수는 측정일 전에 모든 벌집 소비의 번데기 및 유충을 제거한 후 빈 소비상태에서 측정하였다.The feeding solutions of Examples 1 and 2 and Comparative Example 1 were fed for 20 minutes over three times at 0 am, 4 am, and 8 am and delivered to the queen bee. . The spawning number of queen bees was measured in empty consumption after removing pupae and larvae from all honeycomb consumption before the measurement day.
도 3을 참조하면, 비교예 1에 대비하여 실시예 1은 산란수가 40.6 % 증가하였으며, 실시예 2는 산란수가 5.7 % 증가하였다. 따라서 아무것도 처리하지 않은 사양액을 급이하는 것보다 본원발명에 따른 사양액용 조성물을 첨가하여 급이하는 것이 여왕벌의 산란수 증가에 도움이 된다는 것을 확인할 수 있었다.Referring to FIG. 3, compared to Comparative Example 1, the number of scattering in Example 1 was increased by 40.6%, and the number of scattering in Example 2 was increased by 5.7%. Therefore, it was confirmed that feeding by adding the composition for feeding solution according to the present invention rather than feeding the feeding solution without any treatment was helpful in increasing the spawning number of the queen bee.
또한, 사양액용 조성물을 250 배로 희석하여 사용한 실시예 2에 비해 200 배로 희석하여 사용한 실시예 1의 경우가 산란수 증가율이 더욱 큰 것으로 확인되었다. 따라서 사양관리상 산란이 많이 필요한 시기에는 본원발명에 따른 조성물을 사양액에 200 배 희석하여 사용하는 것이 가장 바람직함을 확인할 수 있었다.In addition, it was confirmed that the increase rate of the scattering number was greater in Example 1, which was diluted 200 times and used, compared to Example 2, which was used after diluting the composition for a feeding solution 250 times. Therefore, it was confirmed that it is most preferable to use the composition according to the present invention by diluting it 200 times in the feeding solution at a time when a lot of spawning is required for feeding management.
지금까지 본 발명의 일 실시예에 따른 꿀벌 사양액용 조성물 및 그의 제조방법에 관한 구체적인 실시예에 관하여 설명하였으나, 본 발명의 범위에서 벗어나지 않는 한도 내에서는 여러 가지 실시 변형이 가능함은 자명하다.Until now, specific examples of the composition for a bee feeding solution and a method for preparing the same according to an embodiment of the present invention have been described, but it is obvious that various embodiments can be modified without departing from the scope of the present invention.
그러므로 본 발명의 범위는 설명된 실시예에 국한되어 정해져서는 안 되며, 후술하는 특허청구범위뿐만 아니라 이 특허청구범위와 균등한 것들에 의해 정해져야 한다.Therefore, the scope of the present invention should not be defined by being limited to the described embodiments, and should be defined by the claims and equivalents as well as the claims to be described later.
즉, 전술된 실시예는 모든 면에서 예시적인 것이며, 한정적인 것이 아닌 것으로 이해되어야 하며, 본 발명의 범위는 상세한 설명보다는 후술될 특허청구범위에 의하여 나타내어지며, 그 특허청구범위의 의미 및 범위 그리고 그 등가 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.That is, it should be understood that the above-described embodiments are illustrative in all respects and not limiting, and the scope of the present invention is indicated by the claims to be described later rather than the detailed description, and the meaning and scope of the claims and All changes or modified forms derived from the equivalent concept should be construed as being included in the scope of the present invention.
본 발명에 따른 사양액용 조성물은 식물 추출물에서 유래한 천연물을 이용하여 친환경적으로 꿀벌의 면역력을 증진시킬 수 있다.The composition for feeding liquid according to the present invention can enhance the immunity of bees environmentally by using natural products derived from plant extracts.
또한, 본 발명에 따른 사양액용 조성물을 사용하면 꿀벌의 면역력 증가를 통해 꿀벌의 개체수와 산란수 증가로 양봉 산물의 생산성을 향상시킬 수 있다.In addition, when the composition for feeding liquid according to the present invention is used, the productivity of beekeeping products can be improved by increasing the number of bees and the number of spawning by increasing the immunity of bees.

Claims (10)

  1. 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물, 구연산, L-글루타민산나트륨, 및 식물성 유지를 포함하며, Contains seaweed complex extract, mugwort extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oils,
    상기 해조 복합추출물, 개똥쑥 추출물, 및 프로폴리스 추출물은 40~60 ℃에서 4~8 시간 동안 열수추출되는 것을 특징으로 하는 꿀벌 사양액용 조성물.The seaweed complex extract, wormwood extract, and propolis extract are hot water extract for 4 to 8 hours at 40 to 60 ℃ composition for bee feeding liquid.
  2. 제1항에 있어서,The method of claim 1,
    상기 사양액용 조성물은 사양액에 1/100~1/1000로 희석하여 사용되는 것을 특징으로 하는 꿀벌 사양액용 조성물.The composition for feeding liquid is used by diluting 1/100 to 1/1000 in the feeding liquid.
  3. 제1항에 있어서,The method of claim 1,
    상기 해조 복합추출물은 전체 조성물 대비 0.1~5 중량%로 포함되는 것을 특징으로 하는 꿀벌 사양액용 조성물.The seaweed complex extract is a composition for bee feeding liquid, characterized in that contained in an amount of 0.1 to 5% by weight relative to the total composition.
  4. 제1항에 있어서,The method of claim 1,
    상기 개똥쑥 추출물은 전체 조성물 대비 0.1~0.5 중량%로 포함되는 것을 특징으로 하는 꿀벌 사양액용 조성물.The wormwood extract is a composition for a bee diet, characterized in that contained in an amount of 0.1 to 0.5% by weight relative to the total composition.
  5. 제1항에 있어서,The method of claim 1,
    상기 프로폴리스 추출물은 전체 조성물 대비 0.1~1 중량%로 포함되는 것을 특징으로 하는 꿀벌 사양액용 조성물.The propolis extract composition for bee feeding liquid, characterized in that contained in an amount of 0.1 to 1% by weight relative to the total composition.
  6. 제1항에 있어서,The method of claim 1,
    전체 조성물 대비 상기 구연산은 0.5~3 중량%, 상기 L-글루타민산나트륨은 0.1~1중량%, 및 상기 식물성유지는 0.05~1.5 중량%로 포함되는 것을 특징으로 하는 꿀벌 사양액용 조성물.The composition for bee feeding solution, characterized in that the citric acid is 0.5 to 3% by weight, the sodium L-glutamate is 0.1 to 1% by weight, and the vegetable oil is contained in an amount of 0.05 to 1.5% by weight.
  7. (a) 미역, 파래, 및 김을 혼합하고 40~60 ℃에서 4~8 시간 동안 열수추출하여 해조 복합추출물을 제조하는 단계;(a) mixing seaweed, green laver, and seaweed and hot water extraction at 40-60° C. for 4-8 hours to prepare a seaweed complex extract;
    (b) 개똥쑥을 40~60 ℃에서 4~8 시간 동안 열수추출하여 개똥쑥 추출물을 제조하는 단계;(b) preparing a wormwood extract by hot water extraction at 40 to 60 ℃ for 4 to 8 hours;
    (c) 프로폴리스를 40~60 ℃에서 4~8 시간 동안 열수추출하여 프로폴리스 추출물을 제조하는 단계; 및(c) preparing a propolis extract by extracting propolis with hot water at 40 to 60° C. for 4 to 8 hours; And
    (d) 상기 해조 복합추출물, 개똥쑥 추출물, 프로폴리스 추출물의 혼합추출물에 구연산, L-글루타민산나트륨, 및 식물성 유지를 첨가하여 사양액용 조성물을 제조하는 단계;(d) preparing a composition for feeding liquid by adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, mugwort extract, and propolis extract;
    를 포함하는 꿀벌 사양액용 조성물의 제조방법.Method for producing a composition for a bee feeding liquid comprising a.
  8. 제7항에 있어서,The method of claim 7,
    상기 (a) 단계에서In step (a)
    상기 해조 복합추출물은 미역, 파래, 김과 물을 1:10~100의 중량비로 혼합하여 추출되는 것을 특징으로 하는 꿀벌 사양액용 조성물의 제조방법.The seaweed complex extract is a method for producing a composition for a bee feeding liquid, characterized in that it is extracted by mixing seaweed, green laver, laver and water in a weight ratio of 1:10 to 100.
  9. 제7항에 있어서,The method of claim 7,
    상기 (a) 단계에서, In step (a),
    상기 미역, 파래, 및 김을 1:1:1 또는 2:1:1 또는 1:2:1 또는 1:1:2의 중량비로 혼합하는 것을 특징으로 하는 꿀벌 사양액용 조성물의 제조방법.The method for producing a composition for a bee feeding liquid, characterized in that mixing the seaweed, green onion, and seaweed in a weight ratio of 1:1:1 or 2:1:1 or 1:2:1 or 1:1:2.
  10. 제7항에 있어서,The method of claim 7,
    상기 (b) 단계에서 상기 개똥쑥 추출물은 개똥쑥과 물을 1:10~100의 중량비로 혼합하여 추출되는 것을 특징으로 하며,In the step (b), the wormwood extract is characterized in that it is extracted by mixing the wormwood and water in a weight ratio of 1:10 to 100,
    상기 (c) 단계에서 상기 프로폴리스 추출물은 프로폴리스와 물을 1:10~100 중량비로 혼합하여 추출되는 것을 특징으로 하는 꿀벌 사양액용 조성물의 제조방법.In the step (c), the propolis extract is a method for producing a composition for bee feeding liquid, characterized in that the propolis and water are mixed in a weight ratio of 1:10 to 100 and are extracted.
PCT/KR2020/012519 2019-09-30 2020-09-16 Honeybee feeding solution composition for improving honey collection, and preparation method therefor WO2021066358A1 (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH035785B2 (en) * 1984-10-01 1991-01-28 Nippon Haigo Shiryo Kk
KR20150045105A (en) * 2013-10-18 2015-04-28 원웅희 Bee nutrition fodder of fermented liquefaction extracted from mixed sugar and production method
KR20180015199A (en) * 2018-01-17 2018-02-12 이정훈 Production method of monosaccharide honey containing functional ingredients using natural bees
KR20180079197A (en) * 2016-12-30 2018-07-10 전남대학교산학협력단 A Feed Composition for Insects Containing MSG, A Breeding Method for Insects Using the Same, Insects Obtained Therefrom And Food Composition
KR102089736B1 (en) * 2019-09-30 2020-03-17 주식회사 바이오포스 Composition for bee feeding liquid to improve honey collecting ability and preparing method of thereof
KR102149215B1 (en) * 2019-12-12 2020-08-31 주식회사 바이오포스 Composition for bee feeding liquid to improve honey collecting ability and preparing method of thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH035785B2 (en) * 1984-10-01 1991-01-28 Nippon Haigo Shiryo Kk
KR20150045105A (en) * 2013-10-18 2015-04-28 원웅희 Bee nutrition fodder of fermented liquefaction extracted from mixed sugar and production method
KR20180079197A (en) * 2016-12-30 2018-07-10 전남대학교산학협력단 A Feed Composition for Insects Containing MSG, A Breeding Method for Insects Using the Same, Insects Obtained Therefrom And Food Composition
KR20180015199A (en) * 2018-01-17 2018-02-12 이정훈 Production method of monosaccharide honey containing functional ingredients using natural bees
KR102089736B1 (en) * 2019-09-30 2020-03-17 주식회사 바이오포스 Composition for bee feeding liquid to improve honey collecting ability and preparing method of thereof
KR102149215B1 (en) * 2019-12-12 2020-08-31 주식회사 바이오포스 Composition for bee feeding liquid to improve honey collecting ability and preparing method of thereof

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