KR102089736B1 - Composition for bee feeding liquid to improve honey collecting ability and preparing method of thereof - Google Patents

Abstract

The present invention manufactures a composition for a feeding liquid containing a seaweed complex extract, an Artemisia annua extract, and a propolis extract as active components to improve immunity of honey bees through natural products derived from nature, thereby increasing the number of queen bees and the number of queen bee spawning to increase the productivity of bee farms. The present invention provides the composition for a honeybee feeding liquid which contains the seaweed complex extract, the Artemisia annua extract, the propolis extract, citric acid, sodium L-glutamate, and vegetable oil, wherein the seaweed complex extract, the Artemisia annua extract, and the propolis extract are hot water extracted at 40 to 60°C for 4 to 8 hours.

Description

Composition for bee specification liquid for improving water tightness and its manufacturing method {COMPOSITION FOR BEE FEEDING LIQUID TO IMPROVE HONEY COLLECTING ABILITY AND PREPARING METHOD OF THEREOF}

The present invention relates to a bee specification liquid composition for improving water tightness and a method for manufacturing the same, and more specifically, an environmentally friendly bee specification liquid composition for increasing bee farm productivity by increasing immunity of bees and a method for manufacturing the same It is about.

Beekeeping is traditionally used for the production of honey and bees wax, as well as for the production of royal jelly, pollen, propolis, bee venom, etc. It is used in a variety of ways, from using bees to (bee pollination). The proportion of honey production in total livestock production is not very large, but it is known that 70% of the food we eat is pollinated by bees. Therefore, maintaining the population of bees will have a profound effect on agricultural production.

In the domestic beekeeping industry, the domestic market of bee products reaches about 400 billion won, and the ecological pluralistic functions of bees, such as pollen cultivation of crops, are difficult to quantify. In Korea, the economic value of bees accounts for 6 trillion won, which is 50% of the total 12.5 trillion won of fruit and vegetable production, and the overall ecosystem conservation effect exceeds 50 trillion won. Bees are representative livestock that are concentrated and raised in a factory manner. Depending on the farmhouse, the number of beehives ranges from hundreds to thousands, and in the case of Korea, the number of bee-bongs ranks 20th in the world, making one bee per 45 people. It is known that there are about 2 million bongs in Korea as of 2011.

The most important thing in the specification management of beekeeping is to keep the strength of Bonggun strong. If the strength of the Bonggun is strong, the bee can have immunity against disease, improve water tightness, and prevent the verb during wintering. In order to make Bonggun strong, the first step is to manage spawning in spring, minimize pesticide damage in summer, and minimize the occurrence of viral diseases by managing the humidity inside the beehive. In addition, it is very important to reduce the death of bees by preventing old-age disease and controlling mites after the mining of the major domestic wheat sources such as black ash and chestnut honey.

Management of autumn specifications for wintering is to prepare enough food and prepare young worker bees to prepare for wintering and to secure an apiary favorable to wintering. There are several causes of wintering failure, but among them, the weak group (less than 5,000) often fails to control the temperature in the Bonggun, leaving the prepared food and verbalizing it. Therefore, the most important factor for maintaining the strength of the Bonggun is the spawning rate, which should be maintained throughout the year as well as in the spring and autumn.

However, the method for improving the spawning rate used by domestic beekeepers is limited to using folk medicine using herbal medicines or using animal medicines for spawning agents developed to feed chickens (chicken).

In order to solve the problems as described above, the present invention aims to improve the immunity of bees by preparing a composition for a specification solution comprising a seaweed complex extract, a wormwood wormwood extract, and a propolis extract as an active ingredient.

In addition, the present invention aims to increase the productivity of beekeepers by increasing the number of eggs of bees and the number of eggs of queen bees through eco-friendly compositions derived from plant extracts.

In order to achieve the above object, the present invention includes a seaweed complex extract, thrush extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oil, and the seaweed complex extract, thrush extract, and propolis extract are 40 It provides a composition for a bee specification liquid, characterized in that the hot water extraction for 4-8 hours at ~ 60 ℃.

The specification solution composition may be used by diluting 1/100 to 1/1000 in the specification solution.

The seaweed composite extract may be included in an amount of 0.1 to 5% by weight relative to the total composition.

The thrush wormwood extract may be included in an amount of 0.1 to 0.5% by weight relative to the total composition.

The propolis extract may be included in 0.1 to 1% by weight relative to the total composition.

The citric acid compared to the total composition may be included in 0.5 to 3% by weight, the L-sodium glutamate 0.1 to 1% by weight, and the vegetable oil is 0.05 to 1.5% by weight.

In addition, the present invention comprises the steps of: (a) mixing seaweed, greenery, and seaweed and preparing hot water composite extract by extracting hot water at 40-60 ° C. for 4-8 hours; (B) the step of preparing a wormwood mugwort extract by hot water extraction for 4-8 hours at 40-60 ℃; (c) preparing a propolis extract by extracting hot water at 40-60 ° C. for 4-8 hours; And (d) adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, thrush extract, and propolis extract to prepare a composition for a specification solution. Provides a method.

In the step (a), the seaweed complex extract may be extracted by mixing seaweed, green, seaweed and water in a weight ratio of 1:10 to 100.

In the step (a), the seaweed, green, and seaweed may be mixed in a weight ratio of 1: 1: 1 or 2: 1: 1 or 1: 2: 1 or 1: 1: 2.

In step (b), the thrush can be mixed with water in a weight ratio of 1:10 to 100, and in step (c), propolis can be mixed with water in a weight ratio of 1:10 to 100.

The specification liquid composition according to the present invention has an advantage of enhancing immunity of bees in an environmentally friendly manner by using natural products derived from plant extracts.

In addition, by using the composition for the specification solution according to the present invention, it is possible to improve the productivity of the beekeeping products by increasing the number of eggs and the number of eggs by increasing the immunity of the bees.

1 is a graph showing the temperature change after feeding the composition for the specification solution of Example 1, Example 2, and Comparative Example 1 to bees.
FIG. 2 is a graph showing the change in population over time after feeding the composition for specification solutions of Example 1, Example 2, and Comparative Example 1 to bees.
FIG. 3 is a graph showing the change in scattering number over time after the composition of the specification solution of Example 1, Example 2, and Comparative Example 1 was delivered to a queen bee.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains may easily practice. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art. Accordingly, the embodiments of the present invention can be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below.

It should be noted that in the following description, only parts necessary for understanding the embodiments of the present invention are described, and descriptions of other parts will be omitted without detracting from the gist of the present invention.

The terms or words used in the present specification and claims described below should not be construed as being limited to ordinary or dictionary meanings, and the inventor is appropriate as a concept of terms to describe his or her invention in the best way. It should be interpreted as a meaning and a concept consistent with the technical idea of the present invention based on the principle that it can be defined as such. Therefore, the configuration shown in the embodiments and drawings described in this specification is only a preferred embodiment of the present invention, and does not represent all of the technical spirit of the present invention, and various equivalents that can replace them at the time of this application It should be understood that there may be and variations.

Hereinafter, a composition for a specification solution for increasing immunity of a bee according to an embodiment of the present invention and a method of manufacturing the same will be described in detail.

The present invention includes the seaweed complex extract, thrush extract, propolis extract, citric acid, sodium L-glutamate, and vegetable oil, and the seaweed complex extract, thrush extract, and propolis extract are 4 to 40 ~ 60 ° C. It provides a composition for bee specification liquid, characterized in that the hot water extraction for 8 hours.

Seaweed (seaweed) is a macroalgae (macroalgae) that inhabit the sea, refers to multicellular protozoa that grow in the sea and is distinguished from single-cell microalgae in that it has a size that can be observed with the naked eye. The classification in seaweed is divided into green seaweed, brown seaweed, and red seaweed according to photosynthetic pigments, and it also shows differences in the habitat and components of each.

 The green algae include green, blue, and blue, and are grown in marine or fresh water. The red algae include woodfish, seaweed, and caranigin, and are grown in the ocean.

Brown algae include 톳, seaweed, kelp, rhubarb, and capsicum, and the brown algae show characteristics of containing carbohydrates up to about 30 to 67%, especially on a building basis. Most of the polysaccharides in brown algae are composed of alginic acid. Alginic acid is a major component of the cell membrane or intercellular substance of brown algae. Heteropolysaccharide in which β-D-mannuronic acid (M) and α-L-guluronic acid (G) are bound to α-1, 4 or β-1,4. It is known as.

The seaweed complex extract according to an embodiment of the present invention contains alginic acid as an active ingredient. Alginic acid enhances the production of nitric oxide and the proliferation of granular white blood cells, which can improve the immunity of bees.

The alginic acid may be contained in the range of 7.5 to 500 mg / kg compared to the seaweed complex extract, and if it is outside the above range, it is preferable to be included in the above range since the effect of enhancing the immunity of bees due to alginic acid is minimal.

According to an embodiment of the present invention, the seaweed complex extract may be prepared by mixing seaweed, green onion, and seaweed, and the seaweed, green onion, and seaweed may be 1: 1: 1 or 2: 1: 1 or 1: 2. It is preferably prepared by mixing in a weight ratio of 1: 1 or 1: 1: 2. Since it is possible to extract alginic acid within an effective range when it is mixed in the above weight ratio, it is preferable to mix in the above weight ratio.

As an extraction solvent for extracting the extract, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but water is preferably used.

The seaweed composite extract may be prepared by mixing seaweed, greenery, and seaweed with water in a weight ratio of 1:10 to 100, and extracting them at 40-60 ° C for 4-8 hours. When the weight ratio of the seaweed, green, seaweed and water is outside the above range, the active ingredient of the extract may be extracted in a small amount. In addition, if the extraction temperature is less than 40 ° C, the active ingredient may not be extracted, and if it exceeds 60 ° C, the active ingredient may be extracted in a small amount due to extraction of unnecessary substances.

In the present invention, kelp (Laminaria japonica) is a seaweed belonging to the kelp family (Phaeophyta) kelp (Phaeophyceae) kelp tree (Laminariales) kelp family (Laminariaceae), apparently divided into leaf, stem, and root, and the frond is stripe The shape is long and the central part of the upper part of the blade forms a thick middle part, and both sides of the middle part are vertically flawed. The stem is usually columnar and erect, and the root has a dendritic shape branching from the lower part of the stem to the car. The kelp has been widely used for food in Korea, Japan, and China since ancient times.

In addition, in the present invention, seaweed (Undaria pinnatifida) is a seaweed belonging to the algae of the brown seaweed plant (Phaeophyta) brown seaweed (Phaeophyceae) kelp tree (Laminariales), the base is dendritic, the stem is flat, the upper part Is the middle part of the upper part of the lobe, and the leaf has the right upper lobe. It is distributed in Korea and Japan. It is distributed in a wide area from Baengnyeongdo Island to the end of the East Coast in the west coast. It has been cultivated in Korea and Japan since ancient times.

On the other hand, the green leaf is a seaweed belonging to the family Green, which is rich in iron, methyl methionine, dietary fiber, and chlorophyll components, and is effective for anemia improvement, nicotine neutralization, constipation improvement, diet and bad breath removal. Blue is strong in pollution and grows well in a eutrophic environment, and unlike land plants, it contains a large amount of polysaccharides containing sulfate groups, and is mainly composed of glucurono-rhamnoxyloglycan, Sulfuric acid groups and uronic acid are about 20% and 21%, respectively.

The seaweed composite extract according to an embodiment of the present invention may be included in an amount of 0.1 to 5% by weight compared to the total specification solution composition.

Artemisia annua refers to the annual grass of the chrysanthemum family, Calliflora mugwort, Gaedang mugwort, or Cheongho mugwort. It grows about 1m from the roadside, glade, or riverside, and is called Cheongho because the back side of the leaves is blue in mugwort. In oriental medicine, it is known to be effective in the treatment of fever, colds, school problems, indigestion, dyspepsia and dysentery.

The wormwood wormwood contains an anti-oxidant, anti-inflammatory, anti-cancer, and artemisinin component, a polyphenol compound with immunomodulatory effects. In particular, antioxidant activity is known to contain 50-70% of oregano, which is known as the best material.

The thrush extract according to an embodiment of the present invention contains polyphenol as an active ingredient, and the polyphenol may be contained in an amount of 0.01 to 1000 mg / kg compared to thrush extract. The polyphenol has an antioxidant effect, an antibacterial effect, and an antibiotic effect and can help relieve stress, thereby enhancing honey's immunity.

As an extraction solvent for extracting the extract, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but water is preferably used.

The thrush wormwood extract may be prepared by mixing thrush wormwood with water in a weight ratio of 1:10 to 100 and extracting it at 40-60 ° C for 4-8 hours. When the weight ratio of the wormwood mugwort and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, if the extraction temperature is less than 40 ° C, the active ingredient may not be extracted, and if it exceeds 60 ° C, the active ingredient may be extracted in a small amount due to extraction of unnecessary substances.

The wormwood wormwood extract according to an embodiment of the present invention may be included in an amount of 0.1 to 0.5% by weight compared to the total specification solution composition.

Propolis is a material made by mixing saliva and enzymes with a substance such as resin extracted from various plants for bees to survive and reproduce. Resin, beeswax, essential oil, pollen, etc. Flavonoids and the like.

Propolis is a natural antibiotic, and it is known to contain the most organic and minerals (inorganic salts) and contains about 104 kinds of ingredients. Flavonoids, polyphenols, aromatic carboxylic acids, aromatic aldehydes, etc. are the main active ingredients. This is known and the main action is antibacterial action, antioxidant action, anti-inflammatory action, local anesthesia action, virus growth suppression action, immune regulation action, and the like.

Propolis extract according to an embodiment of the present invention may include a polyphenol as an active ingredient, the polyphenol may be contained in 0.001 ~ 1000 mg / kg compared to the propolis extract. The polyphenol has an antioxidant effect, an antibacterial effect, and an antibiotic effect and can help relieve stress, thereby enhancing honey's immunity.

As an extraction solvent for extracting the extract, water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but water is preferably used.

The propolis extract may be prepared by mixing propolis with water in a weight ratio of 1:10 to 100 and extracting at 40-60 ° C. When the weight ratio of the propolis and water is outside the above range, the active ingredient of the extract may be extracted in a small amount. In addition, if the extraction temperature is less than 40 ° C, the active ingredient may not be extracted, and if it exceeds 60 ° C, the active ingredient may be extracted in a small amount due to extraction of unnecessary substances.

Propolis extract according to an embodiment of the present invention may be included in 0.1 to 1% by weight of the total composition.

The mixture of the seaweed complex extract, thrush extract, and propolis extract may be contained in an amount of 0.3 to 6.5% by weight compared to the total specification solution composition. When the content of the seaweed complex extract, thrush extract, and propolis extract is less than 0.3% by weight compared to the total composition, it is difficult to produce a desired effect in enhancing the immunity of honey. It is not economically desirable because the improvement of is insignificant.

The specification solution composition according to the present invention may further include citric acid, L-sodium glutamate, and vegetable fats and oils in addition to the active ingredients.

The citric acid may be included in 0.5 to 3% by weight compared to the total composition, the L-sodium glutamate may be included in 0.1 to 1% by weight, the vegetable oil may be included in 0.05 to 1.5% by weight.

The specification solution composition may be used by diluting 1/100 to 1/1000 in the specification solution. The specification solution composition may be used with different dilution concentrations depending on the application. For example, when used for the purpose of making a steel group and winter specifications, it is preferable to mix 500 ml of the specification solution composition with 100 l of the specification solution and dilute it to 1/200.

In addition, when used for the purpose of increasing the mite resistance, it is preferable to use a mixture of 250 ml of the specification solution for 100 l of the specification solution and diluting it to 1/400.

In addition, when used for general specifications, it is preferable to mix 333 ml of the specification liquid composition with 100 l of the specification liquid and dilute it to 1/300.

According to another aspect of the present invention, the present invention comprises the steps of: (a) mixing seaweed, greenery, and seaweed and extracting hot water for 4-8 hours at 40-60 ° C. to produce a seaweed complex extract; (B) the step of preparing a wormwood mugwort extract by hot water extraction for 4-8 hours at 40-60 ℃; (c) preparing a propolis extract by extracting hot water at 40-60 ° C. for 4-8 hours; And (d) adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, thrush extract, and propolis extract to prepare a composition for a specification solution. Provides a method.

In the step (a), the seaweed complex extract may be prepared by mixing seaweed, greenery, and seaweed, and the seaweed, greenery, and seaweed may be 1: 1: 1 or 2: 1: 1 or 1: 2: 1 or It is preferably prepared by mixing in a weight ratio of 1: 1: 2.

As the extraction solvent for extracting the extract in step (a), water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but water is preferably used.

The seaweed composite extract may be prepared by mixing seaweed, greenery, and seaweed with water in a weight ratio of 1:10 to 100, and extracting them at 40-60 ° C for 4-8 hours. When the weight ratio of the seaweed, green, seaweed and water is outside the above range, the active ingredient of the extract may be extracted in a small amount. In addition, if the extraction temperature is less than 40 ° C, the active ingredient may not be extracted, and if it exceeds 60 ° C, the active ingredient may be extracted in a small amount due to extraction of unnecessary substances.

As the extraction solvent for extracting the extract in the step (b), water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but water is preferably used.

The thrush wormwood extract may be prepared by mixing thrush wormwood with water in a weight ratio of 1:10 to 100 and extracting it at 40-60 ° C for 4-8 hours. When the weight ratio of the wormwood mugwort and water is out of the above range, the active ingredient of the extract may be extracted in a small amount. In addition, if the extraction temperature is less than 40 ° C, the active ingredient may not be extracted, and if it exceeds 60 ° C, the active ingredient may be extracted in a small amount due to extraction of unnecessary substances.

As the extraction solvent for extracting the extract in step (c), water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used. The mixed solvent is not particularly limited, but water is preferably used.

The propolis extract may be prepared by mixing propolis with water in a weight ratio of 1:10 to 100 and extracting it at 40-60 ° C for 4-8 hours. When the weight ratio of the propolis and water is outside the above range, the active ingredient of the extract may be extracted in a small amount. In addition, if the extraction temperature is less than 40 ° C, the active ingredient may not be extracted, and if it exceeds 60 ° C, the active ingredient may be extracted in a small amount due to extraction of unnecessary substances.

In step (d), citric acid, sodium L-glutamate, and vegetable oil are further added to prepare a composition for a specification solution. The citric acid may be added in an amount of 0.5 to 3% by weight relative to the total composition, the L-sodium glutamate may be added in an amount of 0.1 to 1% by weight, and the vegetable oil may be added in an amount of 0.05 to 1.5% by weight.

The composition for the specification liquid prepared by the above step can be used by diluting it in the specification liquid at 1/100 to 1/1000. The specification solution composition may be used with different dilution concentrations depending on the application. For example, when used for the purpose of making a steel group and the winter specification, it is preferable to mix 500 ml of the specification solution composition with 100 l of the specification solution and dilute it to 1/200.

In addition, when used for the purpose of increasing the mite resistance, it is preferable to use a mixture of 250 ml of the specification solution for 100 l of the specification solution and diluting it to 1/400.

In addition, when used for general specifications, it is preferable to mix 333 ml of the specification solution composition with 100 liters of the specification solution and dilute it to 1/300.

The specification liquid composition according to the present invention has an advantage of enhancing immunity of bees in an environmentally friendly manner by using natural products derived from plant extracts.

In addition, by using the composition for the specification solution according to the present invention, it is possible to improve the productivity of the beekeeping products by increasing the number of eggs and the number of eggs by increasing the immunity of the bees.

Example

Hereinafter, examples will be described in detail to specifically describe the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

<Comparative Example 1>

30% sugar water was prepared and used as a specification solution.

<Example 1>

First, domestic seaweed, green onion, and seaweed were all purchased as organisms to produce a seaweed complex extract. The water was washed three or more times with flowing water to remove salts and foreign matters from seaweed, greens, seaweed, and then freeze-dried by primary freezing. Thereafter, the weight ratio of seaweed, green pepper, and seaweed was mixed to be 1: 1: 1. The mixture and water were mixed so as to be 10% (w / w), immersed for 24 hours, then stirred and extracted at 50 ° C. for 6 hours, and then filtered to prepare an extract.

Next, the leaves of wormwood mugwort were washed with water, and then mixed with the leaves of wormwood mugwort so that water was 10% (w / w), stirred and extracted at 50 ° C. for 6 hours, and then filtered to prepare wormwood wormwood extract. In addition, the propolis powder and water were mixed so as to be 10% (w / w), and the mixture was stirred and extracted at 50 ° C. for 6 hours, and then filtered to prepare a propolis extract.

After mixing the seaweed complex extract, thrush wormwood extract, and propolis extract, citric acid, sodium L-glutamate, and vegetable oil were further added to prepare a composition for a specification solution. The prepared specification solution composition was diluted to 1/200 in 30% sugar water of Comparative Example 1 and added.

<Example 2>

The specification solution composition prepared by the same method as in Example 1 was diluted to 1/250 in 30% sugar water of Comparative Example 1 and added.

Experimental Example 1: Change in temperature of bees after feeding the specifications

The specifications of Examples 1, 2 and Comparative Example 1 were fed to bees for 20 minutes over 0 AM, 4 AM and 8 AM 3 times, and the temperature change after feeding was shown in FIG. 1.

Referring to FIG. 1, it was confirmed that the temperature was increased by 0.5 to 2 ° C. when Example 1 and Example 2 were fed as compared to the case where the specification liquid of Comparative Example 1, which had not processed anything, was fed.

As a result of this, it is judged that the temperature in the system (honeycomb) increased due to the growth and activity of the bee by adding the composition for the specification solution. Since the increase in temperature is used as an index indicating the strength of the Bonggun, when using the composition for the specification solution according to the present invention, the activity of honey can be increased, wintering can be overcome, and the effect of forming the steel group was confirmed.

Experimental Example 2: Changes in the number of bees after feeding the specifications

Specimens of Examples 1 and 2 and Comparative Example 1 were fed to bees for 20 minutes at 0 am, 4 am and 8 am 3 times, and the change in population after feeding is shown in FIG. 2.

Referring to Figure 2, the increase in the population of Comparative Example 1 is 7.3%, Example 1 is 35.1%, Example 2 is 15.9%, the composition for the specification solution according to the present invention rather than feeding the specification solution without processing anything It was confirmed that feeding by adding was helpful in increasing the population of bees.

In addition, in the case of feeding the specification liquid of Comparative Example 1 in which nothing was treated, the population increased after 15 days, but the population decreased again after 30 days, and in the case of Example 2, the composition for the specification liquid was diluted 250 times. After 15 days, it was confirmed that the population decreased. In the case of Example 1 in which the specification solution composition was diluted 200 times, the number of bees gradually increased over time, and as a result, it was confirmed that the number of individuals increased by 35.1%.

Therefore, it was confirmed that it is most preferable to use the composition according to the present invention by diluting it 200 times in the specification solution, and in the season when there is no influence on the increase in population, diluting the diluted multiple by more than 250 times is effective for specification management. I could confirm.

Experimental Example 3: Change in the number of spawning bees after feeding the specifications

The specifications of Examples 1 and 2 and Comparative Example 1 were fed to the queen bee by feeding for 20 minutes over 3 times at 0 AM, 4 AM, and 8 AM, and the change in the number of eggs after feeding was shown in FIG. 3. . The spawning number of the queen bee was measured in the empty consumption state after removing the pupae and larvae of all honeycomb consumption before the measurement day.

Referring to FIG. 3, in comparison with Comparative Example 1, Example 1 increased the scattering number by 40.6%, and Example 2 increased the scattering number by 5.7%. Therefore, it was confirmed that the feeding by adding the composition for the specification liquid according to the present invention rather than feeding the specification liquid not treated with anything helps to increase the number of spawning queen bees.

In addition, it was confirmed that the increase in the number of scattering was greater in the case of Example 1, which was diluted by 200-fold than Example 2, which was used by diluting the composition for the specification solution by 250-fold. Therefore, it was confirmed that it is most preferable to use the composition according to the present invention by diluting it 200 times in the specification solution at a time when scattering is required for specification management.

So far, specific examples of the composition for preparing bee venom and the preparation method thereof according to an embodiment of the present invention have been described, but it is obvious that various modifications can be made without departing from the scope of the present invention.

Therefore, the scope of the present invention should not be limited to the described embodiments, but should be defined not only by the claims to be described later, but also by the claims and equivalents.

That is, the above-described embodiment is illustrative in all respects, and should be understood as not limiting, and the scope of the present invention is indicated by the claims to be described later rather than the detailed description, and the meaning and scope of the claims and It should be construed that any altered or modified form derived from the equivalent concept is included in the scope of the present invention.

Claims (10)

delete delete delete delete delete delete (A) preparing a seaweed complex extract by mixing seaweed, greenery, and seaweed and extracting hot water for 4-8 hours at 40-60 ° C;
(B) the step of preparing a wormwood mugwort extract by hot water extraction for 4-8 hours at 40-60 ℃;
(c) preparing a propolis extract by extracting hot water at 40-60 ° C. for 4-8 hours; And
(d) adding citric acid, sodium L-glutamate, and vegetable oil to the mixed extract of the seaweed complex extract, thrush extract, and propolis extract to prepare a composition for a specification solution;
Method for producing a composition for a bee specification liquid containing a. The method of claim 7,
In step (a) above
The seaweed composite extract is a method of producing a composition for a honeybee specification, characterized in that it is extracted by mixing seaweed, green, seaweed and water in a weight ratio of 1:10 to 100. The method of claim 7,
In step (a),
Method of producing a composition for a honey bee specification, characterized in that the seaweed, green, and seaweed are mixed in a weight ratio of 1: 1: 1 or 2: 1: 1 or 1: 2: 1 or 1: 1: 2. The method of claim 7,
In the step (b), the wormwood wormwood extract is characterized by being extracted by mixing wormwood wormwood and water in a weight ratio of 1:10 to 100,
In the step (c), the propolis extract is a method of preparing a composition for bee specification liquid, characterized in that the propolis and water are extracted by mixing in a weight ratio of 1:10 to 100.

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