WO2021065841A1 - Contenant - Google Patents

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Publication number
WO2021065841A1
WO2021065841A1 PCT/JP2020/036746 JP2020036746W WO2021065841A1 WO 2021065841 A1 WO2021065841 A1 WO 2021065841A1 JP 2020036746 W JP2020036746 W JP 2020036746W WO 2021065841 A1 WO2021065841 A1 WO 2021065841A1
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WO
WIPO (PCT)
Prior art keywords
container
recess
container according
guide thread
pedestal
Prior art date
Application number
PCT/JP2020/036746
Other languages
English (en)
Japanese (ja)
Inventor
久 坂井
恭介 岸本
悠 森
孝 辻
尚一 岡本
Original Assignee
京セラ株式会社
国立研究開発法人理化学研究所
株式会社オーガンテクノロジーズ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 京セラ株式会社, 国立研究開発法人理化学研究所, 株式会社オーガンテクノロジーズ filed Critical 京セラ株式会社
Priority to JP2021551272A priority Critical patent/JPWO2021065841A1/ja
Publication of WO2021065841A1 publication Critical patent/WO2021065841A1/fr
Priority to JP2023109779A priority patent/JP2023118836A/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells

Definitions

  • the present disclosure relates to a container that can store a regenerated hair follicle primordium.
  • AGA androgenetic alopecia
  • a large amount of regenerated hair follicle primordia can be produced from a small amount of sample by culturing epithelial stem cells and mesenchymal stem cells separated from the hair follicle organ.
  • the total number of hair follicles on the patient's scalp can be increased without increasing invasiveness.
  • the container according to the embodiment of the present disclosure is a container that can store a regenerated hair follicle primordium, and has a recess that opens upward and a portion of the inside of the recess that extends upward from the bottom surface of the recess. A plurality of wall portions surrounding the recess are provided, and the region is visible from the outside of the recess.
  • FIG. 1st Embodiment of this disclosure It is sectional drawing of the container which concerns on 1st Embodiment of this disclosure, the state which contained the regenerated hair follicle primordium. It is a perspective view of the container main body part shown in FIG. It is a top view of the container body part shown in FIG. It is sectional drawing of the penetration part (groove) shown in FIG. It is sectional drawing which shows the modification of the pedestal part. It is sectional drawing which shows the modification of the height relationship between a pedestal part and a stopper wall part. It is a perspective view of the lid part shown in FIG. It is sectional drawing which shows the deformation example of the presser foot convex part.
  • the regenerated hair follicle primordia is stored and observed. At the same time, there is a need to develop a container that can be transported.
  • the purpose of the present disclosure is to provide a container for storing, observing, and transporting regenerated hair follicle primordium.
  • the container 1 As shown in FIG. 1, the container 1 according to the first embodiment of the present disclosure has a container main body portion 10 and a lid portion 30.
  • the container 1 can store the regenerated hair follicle primordium 2 inside, observe it, and transport it.
  • the container body 10 and the lid 30 are, for example, transparent polystyrene, Teflon (registered trademark), polypropylene, polyvinyl chloride, polyethylene, cyclic polyolefin, polyethylene terephthalate, polycarbonate, polydimethylsiloxane, polymethylmethacrylate, polyarylate, respectively. It can be made of a synthetic resin material such as polysulfone, polyethersulfone, polyetheretherketone, polyetherimide, polymethylpentene, silicon resin, acrylic, PFA, or a transparent material such as transparent glass. , The material can be changed in various ways.
  • the container body portion 10 and the lid portion 30 may be formed of the same material as each other, or may be formed of different materials from each other.
  • the regenerated hair follicle primordium 2 stored in the container 1 is arranged at the tip 3a of the guide thread 3 and integrated with the guide thread 3.
  • the container 1 can store the guide thread 3 together with the regenerated hair follicle primordium 2.
  • the guide thread 3 penetrates the regenerated hair follicle primordium 2.
  • the regenerated hair follicle primordium 2 can be composed of a cell population containing a plurality of epithelial cells 2a and a cell population containing a plurality of mesenchymal cells 2b.
  • the cell population containing the plurality of mesenchymal cells 2b is arranged on the distal end side (left side in FIG. 1) of the guide thread 3 rather than the cell population containing the plurality of epithelial cells 2a.
  • Epithelial cells means cells collected from epithelial tissue and cells obtained by culturing the collected cells, for example, cells collected from the outermost layer of the outer root sheath of the hair sac-derived bulge region (derived from the bulge region). Epithelial cells), cells collected from the epithelium of adult or fetal skin, epithelial cells derived from pluripotent stem cells, hair follicles, epithelial precursor cells that may differentiate into epithelial stem cells that make up the skin Such stem cells can be mentioned.
  • Mesenchymal cells means cells collected from mesenchymal tissue and cells obtained by culturing the collected cells, for example, cells derived from hair papilla (for example, hair papilla cells), hair matrix cells, and the like. Free of hair root sheath cells, inner hair root sheath cells, outer hair root sheath cells (excluding cells in the outermost layer), adult or fetal skin mesenchymal cells, mesenchymal cells derived from pluripotent stem cells, and blood cells Mesenchymal cells, mesenchymal cells derived from bone marrow, mesenchymal cells inside the jawbone, mesenchymal cells derived from head nerve ridge cells, or mesenchymal progenitor cells or stem cells that may differentiate into these cells, etc. be able to.
  • the "pluripotent stem cell” means a cell having an ability to differentiate into various tissues of a living body, for example, ES cell (Embryonic stem cell), ntES cell (nuclear transfer Embryonic stem cell), EG cell (Embryonic germ). Examples include cells), GS cells (germlyonic stem cells), and iPS cells (induced Puripotent Stem cells).
  • the regenerated hair follicle primordium 2 may have a configuration other than the above. Further, the regenerated hair follicle primordium 2 can be produced, for example, by the method described in Patent Document 1.
  • the guide thread 3 is formed in a thread form (fibrous form) by, for example, a polymer such as nylon, a metal such as stainless steel, a chemical fiber such as carbon fiber or glass fiber, or the like.
  • the guide thread 3 is made of nylon.
  • the length of the guide thread 3 can be 3 to 10 mm, and the diameter of the guide thread 3 can be 0.02 to 1.20 mm. In the present embodiment, the length of the guide thread 3 is 5 mm, and the diameter of the guide thread 3 is 0.5 mm.
  • the culture solution 4 is stored inside the container 1, and the regenerated hair follicle primordium 2 is immersed in the culture solution 4.
  • the culture solution 4 is a liquid prepared for culturing the regenerated hair follicle primordium 2.
  • the culture medium 4 may be a basal medium used for culturing animal cells, and examples thereof include Dulbecco's modified Eagle's medium (DMEM) and Advanced DMEM / F12 medium. Further, in order to efficiently proliferate epidermal cells or mesenchymal cells, cell growth factors such as serum, FGF (Fibroblast growth factor), and EGF (Epidermal growth factor) can be added.
  • Y27632, Noggin, SAG, or the like may be added as an additive for maintaining the function of cells, and antibiotics such as Penicillin and Streptomycin may be added as needed.
  • antibiotics such as Penicillin and Streptomycin
  • the basal medium, serum, cell growth factors, and additives are appropriately changed depending on the properties of epithelial cells or mesenchymal cells.
  • the container main body 10 has a box shape having a recess 11 opened upward.
  • the recess 11 can accommodate the regenerated hair follicle primordium 2.
  • the recess 11 according to the present embodiment is partitioned by, for example, a bottom surface 11a having a rectangular outer shape and four inner wall surfaces 11b, 11c, 11d, and 11e that are perpendicularly connected to the edge of the bottom surface 11a.
  • the opening of the recess 11 has a rectangular shape in which the distance between the inner wall surface 11b and the inner wall surface 11e is larger than the distance between the inner wall surface 11c and the inner wall surface 11d in the plan view shown in FIG. Is a rectangular and annular plane parallel to the bottom surface 11a.
  • the recess 11 is not limited to the above configuration as long as it can accommodate the regenerated hair follicle primordium 2.
  • the area of the bottom surface 11a may be 10 to 200 mm 2 , and the heights of the inner wall surfaces 11b, 11c, 11d, 11e may be set to 3 to 10 mm. Further, the volume of the recess 11 may be set to , for example, 30 to 2000 mm 3.
  • the longitudinal direction of the guide thread 3 is parallel to the bottom surface 11a and perpendicular to the inner wall surfaces 11b and 11e, and the regenerated hair follicle primordium 2 of the guide thread 3 Is housed inside the recess 11 in a posture in which the tip portion 3a on which the thread is arranged faces the inner wall surface 11b.
  • the direction perpendicular to the bottom surface 11a of the container body 10 and the direction from the bottom surface 11a toward the opening of the recess 11 is upward, and the opposite is downward.
  • a first wall portion 12 and a second wall portion 13 are provided inside the recess 11.
  • the first wall portion 12 and the second wall portion 13 are respectively configured as wall portions extending upward from the bottom surface 11a, and the length of the guide thread 3 housed together with the regenerated hair follicle primordium 2 inside the recess 11. They are arranged so as to face each other so as to intersect in the direction.
  • the surface of the first wall portion 12 facing the second wall portion 13 is the stopper surface 12a
  • the surface of the second wall portion 13 facing the first wall portion 12 is the stopper surface 13a. That is, the first wall portion 12 and the second wall portion 13 are stopper wall portions, respectively.
  • the guide thread 3 can be arranged between the first wall portion 12 and the second wall portion 13 (between the stopper surface 12a and the stopper surface 13a). They are located apart.
  • the distance (interval) between the first wall portion 12 and the second wall portion 13 is the length obtained by adding the length of the guide thread 3 and the length of the regenerated hair follicle original group 2 (guide thread 3). Is longer than the length of the guide thread 3) when it penetrates the regenerated hair follicle primordia 2), and a guide extending straight between the first wall portion 12 and the second wall portion 13.
  • the thread 3 can be arranged so that one end in the longitudinal direction thereof faces the first wall portion 12 and the other end faces the second wall portion 13.
  • the first wall portion 12 and the second wall portion 13 can reduce the movement of the guide thread 3 in the longitudinal direction between them.
  • the container main body 10 is provided with the first wall portion 12 and the second wall portion 13 capable of positioning the guide thread 3 in the longitudinal direction inside the recess 11, so that the tip portion 3a of the guide thread 3 is provided. It is possible to reduce the movement of the regenerated hair follicle primordium 2 arranged in the recess 11 inside the recess 11.
  • the distance between the first wall portion 12 and the second wall portion 13 may be larger than the length of the guide thread 3.
  • the distance (interval) between the first wall portion 12 and the second wall portion 13 can be 3.2 to 11.0 mm. In the present embodiment, the distance (interval) is 7 mm.
  • the stopper surface 12a may be inclined inward so as to gradually approach the second wall portion 13 from the upper side to the lower side.
  • the stopper surface 13a may be inclined inward so as to gradually approach the first wall portion 12 from the upper side to the lower side.
  • the inclination angle ⁇ 1 or ⁇ 2 of the stopper surface 12a or the stopper surface 13a can be set to 2 to 50 °. In the present embodiment, the inclination angles ⁇ 1 and ⁇ 2 are 30 °. Further, the heights of the first wall portion 12 and the second wall portion 13 may be set to, for example, 2 to 10 mm.
  • the distance of the upper end between the first wall portion 12 and the second wall portion 13 is larger than that of the guide thread 3, and the distance of the lower end between the first wall portion 12 and the second wall portion 13 is the guide thread. It may be smaller than 3. In this case, for example, the distance of the upper end between the first wall portion 12 and the second wall portion 13 is set to 6 to 16 mm. Further, the distance of the lower end between the first wall portion 12 and the second wall portion 13 is set to 5 to 15 mm.
  • At least one pedestal portion may be provided between the first wall portion 12 and the second wall portion 13.
  • two pedestals 14 and 15 are provided between the first wall portion 12 and the second wall portion 13.
  • the pedestals 14 and 15 are configured as wall portions that extend upward from the bottom surface 11a of the recess 11 and are perpendicular to the longitudinal direction of the guide thread 3 housed in the recess 11.
  • the pedestals 14 and 15 are arranged apart from each other in the longitudinal direction of the guide thread 3 and separated from the inner wall surfaces 11b, 11c, 11d and 11e of the recess 11. Further, the upper ends of the pedestals 14 and 15 are located below the edge 11f of the opening of the recess 11.
  • only one pedestal portion may be provided between the first wall portion 12 and the second wall portion 13. Further, three or more pedestals may be provided between the first wall portion 12 and the second wall portion 13.
  • the distance between the pedestal portion 14 and the pedestal portion 15, the distance between the pedestal portion 15 and the first wall portion 12, and the distance between the pedestal portion 14 and the second wall portion 13 become uniform. It may be arranged as follows. Alternatively, the guide thread 3 may be arranged so that the distance between the pedestal portion 14 and the pedestal portion 15 is increased so that the guide thread 3 can be easily grasped at the center.
  • the distance (inter-core distance) between the center of the pedestal portion 14 and the center of the pedestal portion 15 can be 1 to 5 mm. In the present embodiment, the interval is 2 mm.
  • a penetrating portion on which the guide thread 3 is arranged may be arranged at the upper end of the pedestal portion 14.
  • the penetrating portion penetrates the pedestal portion 14 in the longitudinal direction of the guide thread.
  • a groove 14a as a penetrating portion may be provided.
  • the groove 14a extends in the direction from one side of the first wall portion 12 and the second wall portion 13 toward the other side and is parallel to the bottom surface 11a.
  • the pedestal portion 14 can support a portion of the groove 14a on the base end side opposite to the tip end portion 3a of the guide thread 3.
  • the groove 14a has a V-shaped cross section. As a result, the guide thread 3 can be easily inserted into the groove 14a and can be easily fixed at the bottom of the groove 14a.
  • the groove 14a is not limited to the V shape. Further, protrusions and protrusions may be provided on each surface of the V-shaped groove 14a to prevent the guide thread 3 from coming out of the groove 14a.
  • a penetrating portion on which the guide thread 3 is arranged may be arranged at the upper end of the pedestal portion 15.
  • a groove 15a in which the guide thread 3 is arranged is formed at the upper end of the pedestal portion 15.
  • the groove 15a is configured as a penetrating portion that penetrates from one surface of the pedestal portion 15 facing the side of the first wall portion 12 toward the other surface facing the side of the second wall portion 13.
  • the groove 15a has a V-shaped cross section, and the bottom portion thereof extends coaxially with the bottom portion of the groove 14a of the pedestal portion 14.
  • the pedestal portion 15 can support the tip portion 3a of the guide thread 3 in the groove 15a. Protrusions may be provided on each surface of the V-shaped groove 15a to prevent the guide thread 3 from coming out of the groove 15a.
  • the bottom portions of the grooves 14a and 15a that support the guide thread 3 are located below the upper ends of the first wall portion 12 and the second wall portion 13, respectively.
  • the guide threads 3 arranged in the grooves 14a and 15a are restricted by the first wall portion 12 and the second wall portion 13 so that the movement in the longitudinal direction thereof is regulated by the first wall portion 12 and the second wall portion 12. It can be placed between 13 and 13.
  • the bottom portions of the grooves 14a and 15a that support the guide thread 3 are located below the height of half the height from the bottom surface 11a of the recess 11 to the edge 11f of the opening. As a result, the regenerated hair follicle primordium 2 stored in the recess 11 can be effectively immersed in the culture solution 4 stored in the recess 11.
  • the height of the pedestals 14 and 15 from the bottom surface 11a is larger than the distance between the edge 11f of the opening of the recess 11 and the upper ends of the pedestals 14 and 15.
  • the heights of the pedestals 14 and 15 may be set to, for example, 1.5 to 10 mm.
  • the pedestals 14 and 15 are provided in the container main body 10, and the guide thread 3 is supported by the pedestals 14 and 15 when the regenerated hair follicle primordium 2 is stored in the recess 11.
  • the regenerated hair follicle primordium 2 arranged at the tip portion 3a of the thread 3 can be held at a position separated upward from the bottom surface 11a.
  • the guide threads 3 supported by the pedestals 14 and 15 are provided in the lateral direction (perpendicular to the longitudinal direction of the guide threads 3).
  • the movement in the direction parallel to the bottom surface 11a) can be restricted to reduce the lateral movement of the regenerated hair follicle primordia 2 arranged in the tip portion 3a of the guide thread 3 inside the recess 11. it can.
  • the intermediate portion of the guide thread 3 is floated from the bottom surface 11a between the two pedestals 14 and 15. Can be held in.
  • the intermediate portion of the guide thread 3 can be easily moved between the pedestal portion 14 and the pedestal portion 15 by a tool such as tweezers. Can be picked up. Therefore, the work of taking out the regenerated hair follicle primordium 2 from the container 1 can be easily performed.
  • the portions of the pedestals 14 and 15 above the bottom portions of the grooves 14a and 15a have a shape that becomes thinner toward the upper side, respectively. More specifically, in the portion of the pedestal portion 14 above the bottom portion of the groove 14a, both the surface facing the side of the pedestal portion 15 and the surface facing the side of the second wall portion 13 face upward from below. By inclining so as to approach each other, it becomes thinner upward. On the other hand, the portion of the pedestal portion 15 above the bottom portion of the groove 15a is thinned upward because only the surface facing the side of the pedestal portion 14 is inclined.
  • the inclination angles ⁇ 3 and ⁇ 4 of the portions above the bottom portions of the grooves 14a and 15a of the pedestals 14 and 15 can be set to 30 to 88 °. In the present embodiment, the inclination angles ⁇ 3 and ⁇ 4 are 45 °.
  • the width of the groove 14a of the pedestal portion 14 may be smaller than the width of the groove 15a (penetration portion) of the pedestal portion 15. As a result, the guide thread can be held in the groove 14a.
  • the penetrating portion (groove) 15a may have a first portion 25 and a second portion 26 having a larger inclination angle than the first portion 25.
  • the penetrating portion (groove) 15a may have a two-step inclination.
  • the penetrating portion (groove) 15a can hold the guide thread and can easily immerse the regenerated hair follicle primordium 2 in the culture solution 4.
  • the inclination angle ⁇ 5 of the first part 25 may be, for example, 5 to 50 °
  • the inclination angle ⁇ 6 of the second part 26 may be, for example, 30 to 88 °.
  • the portions above the bottom portions of the grooves 14a and 15a of the pedestals 14 and 15 have a shape that becomes thinner toward the upper side, respectively. Therefore, the container 1 is opened and the regenerated hair is regenerated from the inside of the recess 11.
  • a tool such as tweezers between the pedestal portion 14 and the pedestal portion 15, and the work of picking the guide thread 3 between the pedestal portion 14 and the pedestal portion 15 is further performed. Can be facilitated. Therefore, it is possible to more easily perform the work of picking the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1.
  • the regenerated hair follicle primordium 2 stored in the container 1 picks the base end portion of the guide thread 3 opposite to the tip portion 3a between the pedestal portion 14 and the second wall portion 13 with a tool such as tweezers. It can also be taken out from the recess 11. Also in this case, since the surface of the pedestal portion 14 facing the second wall portion 13 and the stopper surface 13a of the second wall portion 13 are inclined, between the pedestal portion 14 and the pedestal portion 15. Tools such as tweezers can be easily inserted. Therefore, the work of picking the base end portion of the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1 can be easily performed. Further, the pressing convex portion 32 and the pressing convex portion 33 of the lid portion 30 can be easily inserted into the pedestal portion 16 and the pedestal portion 17 facing each other.
  • the length of the guide threads 3 of the pedestals 14 and 15 in the longitudinal direction is such that the support area supported by the pedestals 14 and 15 of the guide threads 3 is not supported by the pedestals 14 and 15 of the guide threads 3. Is also set to be small. As a result, in the state of being stored in the container 1, the area that can be picked by a tool such as tweezers of the guide thread 3 is enlarged, and when the regenerated hair follicle primordium 2 is taken out from the inside of the recess 11, the tweezers or the like is used. The work of picking the guide thread 3 can be performed more easily by the tool of the above.
  • FIG. 5 shows a configuration in which the bottom portion of the groove 14a of the pedestal portion 14 is made higher than the bottom portion of the groove 15a of the pedestal portion 15.
  • the bottom portions of the grooves 14a and 15a of the pedestals 14 and 15 extend coaxially with each other so as to be inclined with respect to the bottom surface 11a.
  • the bottom portions of the grooves 14a and 15a of the pedestals 14 and 15 can have an inclination angle ⁇ 7 with respect to the bottom surface 11a coaxially with each other at 1 to 30 °. In the present embodiment, their inclination angles ⁇ 7 are 7 °.
  • the container 1 is arranged so that the regenerated hair follicle primordium 2 is stored in a predetermined position inside the recess 11 so as to be immersed in the culture solution 4, and the guide thread 3 is arranged closer to the opening of the recess 11.
  • the work of picking the guide thread 3 with a tool such as tweezers can be performed more easily.
  • the lid portion 30 and the regenerated hair follicle primordium 2 are not held in parallel as in the modified example of FIG. 5, the regenerated hair follicle primordium 2 does not stick to the lid portion 30, so that the lid portion 30 can be easily made. Can be taken from the container body 10.
  • the upper ends of the pedestals 14 and 15 may be located below the upper ends of the first wall portion 12 and the second wall portion 13.
  • the guide thread 3 is positioned in the longitudinal direction by the first wall portion 12 and the second wall portion 13 in advance, and the guide thread 3 is placed on the pedestal portion. Since it can be inserted into the grooves 14a and 15a of 14 and 15, the work of storing the regenerated hair follicle primordium 2 and the guide thread 3 in the recess 11 can be facilitated.
  • a pedestal portion 16 is provided between the pedestal portion 14 and the pedestal portion 15 adjacent to the pedestal portions 14 and 15. Further, a pedestal portion 17 is provided adjacent to the pedestal portion 14 and the second wall portion 13.
  • the cradle portions 16 and 17 are configured as convex portions that project upward from the bottom surface 11a of the concave portion 11 and extend perpendicularly to the longitudinal direction of the guide thread 3 housed in the concave portion 11.
  • the heights of the pedestals 16 and 17 are lower than the bottom portions of the grooves 14a and 15a that support the guide threads 3 of the pedestals 14 and 15, and the guide threads 3 and the receivers arranged in the grooves 14a and 15a are received.
  • a predetermined gap is provided between the upper surfaces of the bases 16 and 17.
  • the pedestal portion 14 and the pedestal portion 15 are provided in order to open the container 1 and take out the regenerated hair follicle primordium 2 from the inside of the recess 11.
  • a tool such as tweezers
  • the tip of the tool can be brought into contact with the base portion 16 to hold the guide thread 3 in a position where it can be easily picked. ..
  • a tool such as tweezers is inserted between the pedestal portion 14 and the second wall portion 13, and the tool is used.
  • the tip of the tool can be brought into contact with the base portion 17 to hold the guide thread 3 in a position where it can be easily picked. Therefore, it is possible to more easily perform the work of picking the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1.
  • the length (width) of the guide thread 3 in the plane direction parallel to the bottom surface 11a of the pedestal portion 16 in the direction orthogonal to the longitudinal direction is longer than the length (width) of the pedestal portion 17. More specifically, the length of the pedestal portion 17 is the same as the length of the pedestal portions 14, 15 and the second wall portion 13, whereas the length of the pedestal portion 16 is the length of the pedestal portions 14, 15 And it is longer than the length of the second wall portion 13. This makes it easier to find the pedestal portion 16, and when a tool such as tweezers is inserted between the pedestal portion 14 and the pedestal portion 15 to pick up the guide thread 3, the tip of the tool is inserted during the work. It can be brought into contact with the cradle portion 16. Therefore, it is possible to more easily perform the work of picking the guide thread 3 with a tool such as tweezers and taking out the regenerated hair follicle primordium 2 from the container 1.
  • the container body 10 is provided with a pair of side walls 18 and 19 that are integrally connected to the side of the pedestal 15 and project from the inner walls 11c and 11d toward the inside of the recess 11.
  • the pair of side walls 18 and 19 are configured as wall portions extending upward from the bottom surface 11a of the recess 11 and parallel to the longitudinal direction of the guide thread 3 housed in the recess 11.
  • the pair of side walls 18 and 19 may be the inner wall surfaces 11c and 11d of the recess 11.
  • a storage portion 20 for storing the regenerated hair follicle primordium 2 is provided inside the recess 11.
  • the storage portion 20 is partitioned inside the recess 11 as a region surrounded by a first wall portion 12, a pedestal portion 15, and a pair of side walls 18 and 19, which are configured as wall portions, respectively.
  • the upper ends of the first wall portion 12, the pedestal portion 15, and the pair of side walls 18 and 19 are located below the edge 11f of the opening of the recess 11, respectively, and the culture solution 4 is the culture solution 4.
  • a predetermined amount (for example, 300 ⁇ L) is stored inside the container 1 so that the liquid level is located above the upper ends of the first wall portion 12, the pedestal portion 15, and the pair of side walls 18 and 19.
  • the culture solution 4 stored in the container 1 is arranged in the first wall portion 12, the pedestal portion 15, and the inner storage portion 20 of the pair of side walls 18 and 19 inside the recess 11, and is inside the recess 11. It is also arranged in other areas other than the storage unit 20.
  • the culture solution 4 flows in and out of the storage portion 20 and other regions above the upper ends of the first wall portion 12, the pedestal portion 15, and the pair of side walls 18 and 19. It is continuous as possible. Therefore, the storage unit 20 can maintain the regenerated hair follicle primordium 2 in a state of being immersed in the culture solution 4 so that a predetermined amount of the culture solution 4 is stored.
  • the groove 15a (penetration portion) of the pedestal portion 15 also contributes to the inflow and outflow of the culture solution 4.
  • a second penetrating portion 23 penetrating from the inside to the outside of the storage portion 20 is also arranged between the first wall portion 12 and the side wall 18.
  • a third penetrating portion 24 penetrating from the inside to the outside of the storage portion 20 is also arranged between the first wall portion 12 and the side wall 19. That is, by having the second penetrating portion 23 and the third penetrating portion 24 different from the first penetrating portion (groove 15a) on the plurality of wall portions, the regenerated hair follicle primordium 2 is maintained in a state of being immersed in the culture solution 4. It will be easier.
  • the width of the first penetrating portion (groove 15a) may be smaller than the width of the second penetrating portion 23 and the third penetrating portion 24.
  • the guide thread 3 extends from the storage unit 20 to the outside of the storage unit 20 via the groove 15a. Further, the guide thread 3 further extends outward through the groove 14a.
  • the regenerated hair follicle primordium 2 arranged at the tip portion 3a of the guide thread 3 is stored in the storage portion. It can be held inside 20. As a result, it is possible to reduce the contact of the regenerated hair follicle primordium 2 arranged in the storage portion 20 with the side walls 18, 19 and the like, and reduce the damage of the regenerated hair follicle primordium 2.
  • the distance from the bottom surface 20a of the storage portion 20 to the bottom portion of the groove 15a of the pedestal portion 15 is along the direction perpendicular to the longitudinal direction of the guide thread 3 of the regenerated hair follicle primordium 2 arranged in the storage portion 20. It is larger than half of the diameter (half of the maximum width in the direction perpendicular to the longitudinal direction of the guide thread 3). This distance may be set to, for example, 0.5 to 2 mm. As a result, it is possible to reduce the contact of the regenerated hair follicle primordium 2 arranged in the storage unit 20 with the bottom surface 20a of the storage unit 20.
  • the nutrient of the culture solution 4 is distributed to the regenerated hair follicle primordium 2 arranged in the storage portion 20. Therefore, it is possible to reduce the deterioration of the quality of the regenerated hair follicle primordium 2.
  • the lid portion 30 includes a rectangular plate-shaped lid main body 31 corresponding to the edge 11f of the opening of the recess 11 of the container main body portion 10.
  • the lid body 31 covers the opening of the recess 11 and the container body portion 10 is closed.
  • the lid body 31 may seal the opening of the recess 11 by being liquid-tightly fixed to the entire circumference of the edge 11f of the opening of the recess 11 by means such as welding or adhesion.
  • the lid main body 31 may be provided with at least one pressing convex portion extending downward from the lower surface of the lid main body 31.
  • a pair of pressing protrusions 32, 33 are provided on the lower surface of the lid main body 31.
  • the presser foot convex portions 32 and 33 are arranged in parallel with each other at intervals in the longitudinal direction of the guide thread 3, and when the lid portion 30 is fixed to the container body portion 10, the presser foot convex portions 32 and the pedestal portion 14 It is located in the area between the pedestal portion 15 and the pressing convex portion 33 is located in the region between the pedestal portion 14 and the second wall portion 13.
  • the lid main body 31 may have a configuration in which only one pressing convex portion is provided. Further, the lid main body 31 may be configured to be provided with three or more pressing protrusions.
  • the length of the presser foot convex portion 32 along the longitudinal direction of the guide thread 3 may be smaller than the length of the pedestal portion 16 along the longitudinal direction of the guide thread 3, whereby the presser foot convex portion 32 is placed on the pedestal portion. It can be reliably arranged in the area between the 14 and the pedestal portion 15.
  • the length of the presser foot convex portion 33 along the longitudinal direction of the guide thread 3 may be smaller than the length of the pedestal portion 17 along the longitudinal direction of the guide thread 3, thereby causing the presser foot convex portion 33 to be formed. It can be reliably arranged in the area between the pedestal portion 14 and the second wall portion 13.
  • the length of the pressing convex portion 32 along the longitudinal direction of the guide thread 3 may be smaller than the length of the guide thread 3 between the pedestal portion 14 and the pedestal portion 15 along the longitudinal direction. , The pressing convex portion 32 can be easily inserted between the pedestal portion 14 and the pedestal portion 15.
  • the length of the presser foot convex portion 33 along the longitudinal direction of the guide thread 3 may be smaller than the length of the guide thread 3 between the pedestal portion 14 and the pedestal portion 15 along the longitudinal direction. Thereby, the pressing convex portion 33 can be easily inserted between the pedestal portion 14 and the second wall portion 13.
  • the lower ends of the pressing protrusions 32 and 33 can be configured to be located below the upper ends of the first wall portion 12 and the second wall portion 13 and the upper ends of the pedestal portions 14 and 15, respectively.
  • the guide thread 3 is positioned between the first wall portion 12 and the second wall portion 13 in a state where the regenerated hair follicle primordium 2 is stored in the storage portion 20 inside the recess 11. It can be positioned in the vertical direction.
  • the lower ends of the pressing protrusions 32 and 33 can be configured to be located above the bottom portions of the grooves 14a and 15a that support the guide threads 3 of the pedestals 14 and 15, respectively.
  • the movement of the guide thread 3 arranged in the grooves 14a and 15a of the pedestals 14 and 15 is restricted by the convex portions 32 and 33, and the guide thread 3 is positioned in the vertical direction. Can be done.
  • the pair of pressing convex portions of the lid portion 30 can be gradually lowered in accordance with the bottom portions of the grooves 14a and 15a.
  • the upward movement of the guide thread 3 arranged in the grooves 14a and 15a of the pedestals 14 and 15 is restricted by the pressing protrusions 32 and 33.
  • the guide thread 3 can be positioned in the vertical direction.
  • the guide threads 3 arranged in the grooves 14a and 15a of the pedestals 14 and 15 can be tilted and arranged, and the regenerated hair follicle primordium 2 with the guide threads 3 is attached to the lid portion 30 during transportation and storage. It is possible to suppress sticking and make it easier to remove the regenerated hair follicle primordium 2 after removing the lid portion 30.
  • the lower surface of the pressing convex portion 32 may be brought into contact with the upper surface of the pedestal portion 14.
  • the upper surface of the pedestal portion 14 is preferably a flat surface parallel to the bottom surface 11a.
  • the movement of the guide thread 3 arranged in the groove 14a of the pedestal portion 14 upward is restricted by the convex portion 32 within the range of the groove 14a, and the guide thread is restricted. 3 can be positioned in the vertical direction within the range inside the groove 14a.
  • a plurality of pressing protrusions 32 and a pedestal portion 14 facing the pressing protrusions 32 may be provided and brought into contact with each other.
  • the storage portion 20 is visible from the outside of the recess 11. That is, it suffices that the container body 10 and the like have translucency so that the storage portion 20 can be visually recognized.
  • the container body 10 and the lid 30 are made of a transparent material as a whole, and the storage portion 20 can be visually recognized from an arbitrary direction outside the recess 11.
  • the regenerated hair follicle primordium 2 is housed in the recess 11, and after the opening of the recess 11 is closed by the lid portion 30, the regenerated hair follicle primordium 2 is visually or photographed from the outside through the flat surface 35a or the flat surface 22a of the container 1. It is possible to confirm the state by observing by such means.
  • the container body 10 and the lid 30 may be made of, for example, a material having a light transmittance of 70% or more.
  • a recess 21 is provided in the container body 10 so that the regenerated hair follicle primordium 2 stored in the storage portion 20 can be observed more effectively.
  • the recessed portion 21 is provided at a position corresponding to the storage portion 20 of the container main body portion 10, and is recessed so as to open to the outside of the recessed portion 11 and narrow the storage portion 20. That is, for example, the recessed portion 21 may be located on the outer surface of the container main body portion 10 or may be located on the bottom surface.
  • the bottom wall of the container body 10 in the portion where the recess 21 is provided is a flat window wall 22, and the flat surface 22a facing the outside of the window wall 22 is located above the outer bottom surface 11g of the recess 11. There is. As a result, the vertical distance from the flat surface 22a to the bottom surface 20a of the storage unit 20 is smaller than the vertical distance from the outer bottom surface 11g of the recess 11 to the bottom surface 20a of the storage unit 20.
  • the recessed portion 21 is located so that the depth of the storage portion 20 (region) is shallower than that of the other regions.
  • the recessed portion 21 is located on the outer bottom surface of the recess 11 so that the distance between the outside of the container body 10 and the bottom surface of the recess 11 is small. That is, it is recessed upward from the outer bottom surface 11g of the recess 11 so as to open to the outside of the recess 11.
  • the distance between the outside of the container main body portion 10 and the bottom surface 20a of the storage portion 20 of the concave portion 11 is set to the recessed portion 21. It can be made smaller than the case where is not provided. Therefore, the downwardly facing portion of the regenerated hair follicle primordium 2 stored in the storage portion 20 can be observed from the side of the plane 22a through the window wall 22 from a closer position. The observation can be performed using, for example, a microscope.
  • the regenerated hair follicle primordium 2 stored in the storage portion 20 is observed from the lower side via the window wall 22 and also from the side (upper side) of the lid portion 30.
  • the lid portion 30 is provided with a second recessed portion 34 so that the lid portion 30 can also be observed.
  • the second recessed portion 34 is provided at a position corresponding to the storage portion 20 of the lid portion 30, and is opened upward. In other words, the second recessed portion 34 is recessed downward with respect to the lid main body 31.
  • the bottom portion of the second recessed portion 34 is a flat window wall 35, and the vertical distance from the flat surface 35a facing the outside of the window wall 35 to the storage portion 20 is from the upper surface of the lid main body 31 to the storage portion 20. It is smaller than the vertical distance of.
  • the distance between the outside of the lid portion 30 and the storage portion 20 of the recess 11 is larger than that in the case where the second recess portion 34 is not provided. Can also be made smaller. Therefore, the portion of the regenerated hair follicle primordium 2 stored in the storage portion 20 facing upward can be observed from a closer position through the window wall 35 from the side of the plane 35a. The observation can also be performed using, for example, a microscope.
  • the regenerated hair follicle primordium 2 is made clearer by reducing the air layer above the culture solution 4 between the storage portion 20 and the outside of the window wall 35. Can be observed.
  • the container body 10 and the lid 30 are made transparent as a whole, but if at least the window wall 22 of the container body 10 and the window wall 35 of the lid 30 are transparent, other The portion of may be opaque or transparent.
  • FIG. 9 is a cross-sectional view of the container 100 according to the second embodiment of the present disclosure in a state in which the regenerated hair follicle primordium 2 is housed
  • FIG. 10 is a perspective view of the container body 10 shown in FIG.
  • FIG. 11 is a plan view of the container body 10 shown in FIG.
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • the container 100 according to the second embodiment of the present disclosure shown in FIG. 9 is replaced with a lid portion 30 having a structure in which the lid main body 31 is provided with a pair of pressing convex portions 32, 33 as a member for closing the opening of the concave portion 11. Therefore, the lid 40 formed in the form of a thin and flexible sheet made of a synthetic resin material, an aluminum alloy, or the like is used.
  • the lid 40 is liquid-tightly fixed to the entire circumference of the edge 11f of the opening of the recess 11 by means of welding, adhesion, or the like to cover and seal the opening of the recess 11.
  • the upper ends of the first wall portion 12 and the second wall portion 13 provided in the container main body portion 10 are located at the same height as the edge 11f of the opening of the recess 11, and the lid 40 is the first wall portion. It is in contact with the upper ends of 12 and the second wall portion 13. It is sufficient that either the first wall portion 12 or the second wall portion 13 is in contact with each other.
  • the upper ends of the pair of pedestals 14 and 15 provided on the container body 10 are also located at the same height as the edge 11f of the opening of the recess 11, and the lid 40 is located on the upper ends of the pair of pedestals 14 and 15. Are also in contact.
  • the lid 40 By making the lid 40 in contact with the upper ends of the pair of pedestals 14 and 15, the openings of the grooves 14a and 15a can be closed by the lid 40. Therefore, the guide thread 3 can be held inside the grooves 14a and 15a without providing the lid 40 with the portions corresponding to the pressing protrusions 32 and 33.
  • the guide thread 3 is provided along the grooves 14a and 15a of the pair of pedestals 14 and 15 at the upper ends of the grooves 14a and 15a. May move to the side.
  • the culture solution 4 fills the entire inside of the recess 11, and the liquid level thereof is located at the same height as the edge 11f of the opening of the recess 11. Therefore, even if the guide thread 3 moves to the upper end side of the grooves 14a and 15a, the regenerated hair follicle primordium 2 can be maintained in a state of being immersed in the culture solution 4.
  • the guide thread 3 moves to the upper end side of the grooves 14a and 15a.
  • the guide thread 3 can be positioned in the longitudinal direction by the first wall portion 12 and the second wall portion 13.
  • the grooves 14a and 15a of the pedestals 14 and 15 have lengths directed downward from the upper ends of the pedestals 14 and 15 in directions perpendicular to the longitudinal direction of the guide thread 3 of the regenerated hair follicle primordium 2, respectively. It can be configured to be longer than the radius along it. Thereby, it is possible to reduce the contact of the regenerated hair follicle primordium 2 with the lid 40 in a state where the guide thread 3 is supported by the grooves 14a and 15a.
  • the grooves 14a and 15a of the pedestals 14 and 15 have lengths directed downward from the upper ends of the pedestals 14 and 15 in directions perpendicular to the longitudinal direction of the guide thread 3 of the regenerated hair follicle primordium 2, respectively. It can be configured to be longer than half the maximum facing width. Thereby, it is possible to reduce the contact of the regenerated hair follicle primordium 2 with the lid 40 in a state where the guide thread 3 is supported by the grooves 14a and 15a.
  • the upper ends of the first wall portion 12 and the second wall portion 13 and the upper ends of the pair of pedestal portions 14 and 15 may be fixed to the lid 40 by means such as welding and adhesion, respectively.
  • FIG. 12 is a cross-sectional view of the container 200 according to the third embodiment of the present disclosure in a state in which the regenerated hair follicle primordium 2 is housed
  • FIG. 13 is a perspective view of the container body 10 shown in FIG. 14 is a cross-sectional view of the portion of the window wall 22 shown in FIG. 12,
  • FIG. 15 is a perspective view of the lid portion 30 shown in FIG.
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • a portion of the regenerated hair follicle primordium 2 housed in the storage unit 20 facing downward can be observed from a plurality of directions. It is a thing.
  • the window wall 22 of the recessed portion 21 provided in the container main body portion 10 has two outer inclined surfaces 22b and 22c in the lower portion of the storage portion 20.
  • the bottom surface 20a of the storage portion 20 inside the recess 11 has a V-shaped groove shape whose depth increases toward the center portion.
  • the side walls 18 and 19 that partition the storage portion 20 are inclined with respect to the bottom surface 11a, respectively, and the surfaces of the side walls 18 and 19 form an inner inclined surface of the bottom surface 20a, respectively.
  • the outer inclined surfaces 22b and 22c of the recessed portion 21 are located along the inner inclined surfaces formed by the surfaces of the side walls 18 and 19, respectively.
  • the window wall 22 has a V-shaped cross section having a constant thickness.
  • the side walls 18 and 19 that partition the storage portion 20 can have an inclination angle ⁇ 8 with respect to the bottom surface 11a of 30 to 60 °, respectively. In this embodiment, their tilt angle is 45 °.
  • a portion of the regenerated hair follicle primordium 2 housed in the storage portion 20 facing downward can be observed through the window wall 22 from a direction perpendicular to the outer inclined surface 22b, and the outer inclined surface 22c can be observed. It can be observed through the window wall 22 from a direction perpendicular to the window wall 22. Thereby, the regenerated hair follicle primordium 2 can be observed from two directions. The observation can also be performed using, for example, a microscope.
  • the regenerated hair follicle primordium 2 stored in the storage portion 20 can be observed from two directions, so that the lid portion 30 is a second recessed portion.
  • a configuration not provided with 34 is used.
  • a container having a second recess 34 as shown in FIGS. 1 and 7 can be used.
  • the regenerated hair follicle primordium 2 stored in the storage unit 20 can be observed from a total of three directions, two directions on the lower side and one direction on the upper side.
  • the window wall 22 is provided with two outer inclined surfaces 22b and 22c, but the regenerated hair follicle primordium 2 is provided with three or more outer inclined surfaces 22b and stored in the storage portion 20.
  • the downwardly facing portion may be configured to be observable from three or more directions.
  • FIG. 16 is a cross-sectional view of the container 300 according to the fourth embodiment of the present disclosure in a state in which the regenerated hair follicle primordium 2 is housed
  • FIG. 17 is a perspective view of the container body 10 shown in FIG.
  • FIG. 18 is a cross-sectional view of the portion of the window wall 22 shown in FIG.
  • the members corresponding to the above-mentioned members are designated by the same reference numerals, and the description thereof will be omitted again.
  • the container 300 according to the fourth embodiment of the present disclosure shown in FIG. 16 is a member for closing the opening of the recess 11 in the container 200 according to the third embodiment, instead of the lid portion 30, which is shown in FIG.
  • the lid 40 formed in the form of a thin and flexible sheet made of a synthetic resin material, an aluminum alloy, or the like is used.
  • the lid 40 is liquid-tightly fixed to the entire circumference of the edge 11f of the opening of the recess 11 by means of welding, adhesion, or the like to cover and seal the opening of the recess 11.
  • the upper ends of the first wall portion 12 and the second wall portion 13 provided in the container main body portion 10 are openings of recesses 11.
  • the lid 40 is located at the same height as the edge 11f of the lid 40, and is in contact with the upper ends of the first wall portion 12 and the second wall portion 13.
  • the upper ends of the pair of pedestals 14 and 15 provided on the container body 10 are also located at the same height as the edge 11f of the opening of the recess 11, and the lid 40 is located on the upper ends of the pair of pedestals 14 and 15. They are in contact with each other to close the openings of the grooves 14a and 15a.
  • the culture solution 4 fills the entire inside of the recess 11, and the liquid level thereof is located at the same height as the edge 11f of the opening of the recess 11.
  • both ends of the recessed portion 21 may be provided with inclined surfaces that descend from the ends of the window wall 22 toward the inner wall surface 11c and the inner wall surface 11d. As a result, the amount of the culture solution 4 added to the recess 11 side of the first wall portion 12 and the recess 11 side of the second wall portion 13 can be increased.
  • a container having flat surfaces provided at both ends of the recessed portion 21 can be used as shown in FIG.
  • the containers 1, 100, 200, and 300 have a recess 11 that opens upward, and a plurality of wall portions 12, 15 that extend upward from the bottom surface 11a of the recess 11 and surround a part of the inside of the recess 11. , 18, 19, and the region is visible from the outside of the recess 11, and other configurations or shapes of members can be variously changed.
  • the penetrating portion is a groove
  • the penetrating portion may be a through hole

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)

Abstract

Ce contenant (1, 100, 200, 300) peut recevoir un primordium de follicule pileux régénéré (2) et est caractérisé en ce qu'il comporte : une partie évidée (11) ayant une ouverture au niveau de sa partie supérieure ; et une pluralité de parois (12, 15, 18, 19) qui s'étendent vers le haut à partir d'une surface inférieure (11a) de la partie évidée (11) de manière à entourer une zone partielle (20) de l'intérieur de la partie évidée (11), ladite zone (20) étant visible depuis l'extérieur de la partie évidée (11).
PCT/JP2020/036746 2019-09-30 2020-09-28 Contenant WO2021065841A1 (fr)

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JP2021551272A JPWO2021065841A1 (fr) 2019-09-30 2020-09-28
JP2023109779A JP2023118836A (ja) 2019-09-30 2023-07-04 容器

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JP2019180865 2019-09-30
JP2019-180896 2019-09-30
JP2019-180865 2019-09-30
JP2019180896 2019-09-30
JP2019-180881 2019-09-30
JP2019180881 2019-09-30
JP2019-180848 2019-09-30
JP2019-180866 2019-09-30
JP2019180866 2019-09-30
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012108069A1 (fr) * 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ Procédé pour produire une ébauche d'organe régénératrice pourvue d'un guide pour transplantation, composition contenant une ébauche d'organe régénératrice pourvue d'un guide pour transplantation produite par celui-ci, et procédé pour transplanter une ébauche d'organe régénératrice pourvue d'un guide pour transplantation
JP2015171381A (ja) * 2009-02-09 2015-10-01 大日本印刷株式会社 細胞培養容器
JP2017063744A (ja) * 2015-10-01 2017-04-06 大日本印刷株式会社 細胞取扱容器
JP2017216967A (ja) * 2016-06-09 2017-12-14 大日本印刷株式会社 細胞培養容器

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2567564Y2 (ja) * 1992-04-17 1998-04-02 住友ベークライト株式会社 培養用器具
JPH114679A (ja) * 1997-06-18 1999-01-12 Showa Yakuhin Kako Kk 化学的及び微生物学的試験用用具

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015171381A (ja) * 2009-02-09 2015-10-01 大日本印刷株式会社 細胞培養容器
WO2012108069A1 (fr) * 2011-02-09 2012-08-16 株式会社オーガンテクノロジーズ Procédé pour produire une ébauche d'organe régénératrice pourvue d'un guide pour transplantation, composition contenant une ébauche d'organe régénératrice pourvue d'un guide pour transplantation produite par celui-ci, et procédé pour transplanter une ébauche d'organe régénératrice pourvue d'un guide pour transplantation
JP2017063744A (ja) * 2015-10-01 2017-04-06 大日本印刷株式会社 細胞取扱容器
JP2017216967A (ja) * 2016-06-09 2017-12-14 大日本印刷株式会社 細胞培養容器

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