WO2021042447A1

WO2021042447A1 - Micropore pressure-equalizing anti-capillary fast-sampling fluid test device

Info

Publication number: WO2021042447A1
Application number: PCT/CN2019/110908
Authority: WO
Inventors: 王海剑; 陆维克; 张晨; 高飞
Original assignee: 杭州奥泰生物技术股份有限公司
Priority date: 2019-09-06
Filing date: 2019-10-13
Publication date: 2021-03-11
Also published as: CN210953503U

Abstract

Disclosed is a micropore pressure-equalizing anti-capillary fast-sampling fluid test device. The test device comprises a bearing sheet (4), test paper placement grooves (2) are provided in a working side face of the bearing sheet (4), reagent strips are arranged in the test paper placement grooves (2), exhausting and pressure-equalizing holes (7) are provided in the bottom ends of the test paper placement grooves (2), and transverse anti-capillary grooves are provided in the bottom of the test paper placement grooves (2). Air bubbles are prone to be generated at a liquid-collecting end of the bottom of a placement groove of traditional fluid test inserts, such that the test speed is greatly reduced. The test device greatly improves the sampling speed and the test efficiency by providing the exhausting and pressure-equalizing holes (7) at the bottom of the test paper placement grooves (2), and solves the problem of the judgment of a result being influenced by water drops formed by fogging in a cavity. According to the device, three transverse anti-capillary grooves are additionally formed in the bottom of each test paper placement groove (2), thereby solving the problem of an NC membrane reaction area being infiltrated before a release area, and preventing an abnormal chromatography result.

Description

Micropore uniform pressure and capillary fast sampling fluid detection device

Technical field

The utility model belongs to the technical field of fluid detection, and in particular relates to a microporous uniform pressure anti-capillary fluid detection insert.

Background technique

Although the traditional fluid detection insert can accurately detect the results, its sampling speed is slow and the chromatography is uneven. Often because the liquid sampling end at the bottom of the placement tank is prone to air bubbles, the pressure in the placement tank cavity is too high, which affects the reagent sampling speed. When the temperature of the reaction liquid is higher than the outside temperature, it is easy to form water vapor on the inner wall of the cavity and affect the subsequent interpretation. In addition, the liquid tends to take advantage of the capillary action on the back of the reagent to absorb faster than the front sample pad, causing the NC membrane reaction area to be wetted earlier than the gold label release area, which affects the normal bottom-up chromatography reaction of the reagent and cannot proceed smoothly during use. Chromatography of fluid samples.

Summary of the invention

The purpose of the utility model is to provide a fluid detection device for microporous uniform pressure and capillary sampling prevention.

The utility model includes a bearing sheet. A test paper placement groove is provided on the working side of the supporting sheet. Reagent strips are arranged in the test paper placement grooves. The bottom end of the test paper placement groove is provided with an exhaust pressure equalizing hole. The bottom of the test paper placement groove is provided with a transverse anti-capillary groove.

Preferably, the top of the test paper placement groove is provided with a mushroom cavity, and the width of the mushroom cavity gradually expands along the direction from the bottom end to the top end of the test paper placement groove. The top end of the mushroom cavity is flat.

Preferably, the bottom end of the test paper placement groove is provided with a cornice opening, and the top end is a beveled wedge shape.

Preferably, the top and bottom ends of the side walls of the test paper placement groove are respectively provided with upper staples and lower staples.

Preferably, there are n test paper placement grooves in total, and n≤20; the n test paper placement grooves are all connected to the bottom edge of the carrier sheet. The bottom end of the reagent strip protrudes out of the corresponding test paper placement slot.

Preferably, the length direction of the transverse anti-capillary groove is parallel to the width direction of the test paper placement groove.

Preferably, the exhaust pressure equalizing holes are located at the symmetry planes corresponding to the two sides of the test paper placement groove.

Preferably, the present invention also includes a cover sheet; the cover sheet covers the working side of the carrier sheet; the top edge of the cover sheet is aligned with the top edge of the carrier sheet; the distance from the bottom edge to the top edge of the cover sheet is greater than the bottom edge of the carrier sheet The distance to the top edge.

Preferably, the cover sheet is made of transparent material.

Preferably, an observation window corresponding to the test paper placement groove is opened on the cover sheet.

The beneficial effects of the utility model are:

1. The present utility model greatly improves the sampling speed of the present utility model by setting the exhaust pressure equalizing hole at the bottom of the test paper placement groove, improves the inspection efficiency, and solves the problem of the interpretation of the result caused by fogging in the cavity. problem.

2. The present utility model solves the problem that the NC membrane reaction zone is infiltrated before the release zone by adding three transverse anti-capillary grooves at the bottom of the test paper placement groove, and avoids abnormal chromatography results.

3. The utility model adds cornices at the bottom of the test paper placement groove and expands the top to form a "mushroom head", which makes the assembly of the reagents of the utility model more convenient, stable and efficient.

4. The utility model can complete the sampling of multiple reagent strips at the same time, thereby realizing the simultaneous detection of multiple indicators of the same sample.

Description of the drawings

Figure 1 is a schematic diagram of the overall structure of embodiment 1 of the utility model;

Figure 2 is a schematic side view of the overall structure in embodiment 1 of the utility model;

Fig. 3 is a schematic front view of the test paper placement groove in embodiment 1 of the utility model.

[Corrected according to Rule 91 02.12.2019]
Fig. 4 is a schematic side view of the test paper placement groove in embodiment 1 of the utility model.

Fig. 5 is a schematic side view of the test paper placement groove in embodiment 3 of the present invention.

detailed description

Hereinafter, the utility model will be further explained in conjunction with the accompanying drawings.

Example 1

As shown in Figures 1 and 2, a microporous pressure equalizing and capillary fast sampling fluid detection device includes a cover sheet 1 and a carrier sheet 4. The supporting sheet 4 has a fan ring shape. The working side of the supporting sheet 4 is provided with n test paper placement grooves 2 arranged in sequence, n=14. The n test paper placement grooves 2 are all connected with the bottom edge of the supporting sheet 4. The n test paper placement grooves 2 are all arranged along the radial direction of the bottom edge of the carrier sheet 4. Reagent strips are provided in the n strips of test paper placement tank 2. The reagent strip adopts the colloidal gold reagent strip. The bottom end of the reagent strip protrudes out of the corresponding test paper placement slot 2. The upper and lower ends of the side wall of the test strip placement groove 2 are provided with upper staples 2-1 and lower staples 2-2. The upper staples 2-1 and the lower staples 2-2 can jam the reagent strip and prevent the reagent strip from being along the test strip. The installation slot 2 slides in the longitudinal direction.

As shown in FIGS. 1, 3 and 4, a mushroom cavity 5 is provided at the top of the test paper placement groove 2, and the width of the mushroom cavity 5 gradually expands along the direction from the bottom end to the top end of the test paper placement groove 2. The top end surface of the mushroom cavity 5 is flat. As the tip space of the test paper placement groove 2 is enlarged, compared with the test paper groove with chamfered tops in the prior art, the test paper placement groove 2 of the present invention can ensure that all reagent strips are better installed on the same horizontal line, thereby improving the detection accuracy , And better observe the test results. The bottom end of each test paper placement groove 2 is provided with a cornice opening. The cross-sectional area of the cornice opening increases sequentially from the inside to the outside, which is conducive to the entry of the tested sample liquid. The bottom end of each test paper placement groove 2 is provided with an exhaust pressure equalizing hole 7. The exhaust pressure equalizing hole 7 is a through hole. The exhaust pressure equalizing holes 7 are located at the symmetry planes corresponding to the two sides of the test paper placement groove 2.

When bubbles are generated at the bottom edge of the eaves opening of the test paper placement groove 2, since the exhaust pressure equalization hole 7 is connected to the tested sample liquid outside the test paper placement groove 2, the exhaust pressure equalization hole 7 can guide the bubbles at the eaves opening Into the sample liquid to be tested, so as to avoid the situation that the sampling stops or the sampling speed is too slow. At the same time, the discharge of air bubbles from the exhaust pressure equalizing hole 7 can also reduce the fogging of the cavity and the formation of water droplets on the wall.

The bottom of the test paper placement groove 2 is provided with three transverse anti-capillary grooves arranged in sequence along the length direction of the test paper placement groove 2 at intervals. The longitudinal direction of the transverse anti-capillary groove is parallel to the width direction of the corresponding test paper placement groove 2. When the tested sample solution rises along the gap between the bottom surface of the test paper placement groove 2 and the reagent strip, the three transverse anti-capillary grooves can store part of the tested sample solution to prevent the tested sample solution from rising too fast and reacting on the NC membrane of the reagent strip. The area is soaked by the test sample before the release area is wet, resulting in abnormal chromatographic results.

The cover sheet 1 has a fan ring shape. Cover sheet 1 uses transparent polyethylene. The cover sheet 1 covers the working side of the carrier sheet 4. The top edge of the cover sheet 1 is aligned with the top edge of the carrier sheet 4. The outer diameter of the cover sheet 1 is equal to the outer diameter of the carrier sheet 4. The inner diameter of the cover sheet 1 is smaller than the inner diameter of the carrier sheet 4, so that the bottom edge of the cover sheet 1 exceeds the bottom edge of the carrier sheet 4, so as to protect the reagent strips protruding out of the test paper placement groove 2.

As a preferred technical solution, the carrier sheet 4 is a molded plastic product.

The working principle of the utility model is as follows:

The user immerses the bottom of the cover sheet 1 and the supporting sheet 4 into the tested sample solution and then takes it out; during the immersion process, the tested sample solution rises along the gap in the test paper placement groove 2 and is absorbed by the reagent strip. The reagent strip reacts under the action of the sample liquid to be tested. When air bubbles are generated at the bottom edge of the test paper arranging groove 2, the air bubbles are discharged from the exhaust pressure equalizing hole, so that the reagent strip absorbs the test sample liquid more quickly and evenly. When the size of the container for the sample liquid to be tested is small, since the carrier sheet 4 is a flexible sheet-like plastic and has a fan ring shape, it can be rolled into a funnel shape, and the bottom extends into the container for the sample liquid to be tested In, sampling is achieved.

In addition, the three horizontal anti-capillary grooves on the test paper placement groove 2 can prevent capillary phenomenon on the back of the reagent strip, so as to ensure that the tested sample liquid first infiltrates the release area and then infiltrates the NC membrane reaction area.

Example 2

The difference between this embodiment and embodiment 1 is that the cover sheet 1 is made of a non-transparent material. The cover sheet 1 is provided with n observation windows corresponding to the n test paper placement slots 2 respectively.

Example 3

As shown in FIG. 5, the difference between this embodiment and the first embodiment is that the top end of the test paper placement groove 2 is an oblique wedge shape 8, that is, the depth gradually decreases along the direction from the bottom end to the top end of the test paper placement groove 2. The top of the beveled wedge 8 can facilitate the insertion and fixation of the reagent strip.

Claims

A microporous uniform pressure and anti-capillary fast sampling fluid detection device, comprising a bearing sheet; characterized in that: a test paper placement groove is arranged on the working side of the carrier sheet; a reagent strip is arranged in the test paper placement groove; The bottom end is provided with an exhaust pressure equalizing hole; the bottom of the test paper placement groove is provided with a transverse anti-capillary groove.
The microporous uniform pressure and anti-capillary fast sampling fluid detection device according to claim 1, wherein the top of the test paper placement groove is provided with a mushroom cavity, and the width of the mushroom cavity is along the bottom end of the test paper placement groove. The direction to the top gradually expands; the top end of the mushroom cavity is flat.
The microporous constant pressure and anti-capillary fast sampling fluid detection device according to claim 1, wherein the bottom end of the test paper placement groove is provided with a cornice opening, and the top end is an oblique wedge shape.
The microporous constant pressure and capillary fast sampling fluid detection device according to claim 1, wherein the top and bottom ends of the side walls of the test paper placement groove are respectively provided with upper staples and lower staples.
The microporous constant pressure anti-capillary fast sampling fluid detection device according to claim 1, wherein there are n test paper placement grooves in total, n≤20; the n test paper placement grooves are all connected to the bottom of the carrier sheet The edges are connected; the bottom end of the reagent strip protrudes out of the corresponding test paper placement groove.
The microporous constant pressure anti-capillary fast sampling fluid detection device according to claim 1, wherein the length direction of the transverse anti-capillary groove is parallel to the width direction of the test paper placement groove.
The microporous pressure equalizing and anti-capillary fast sampling fluid detection device according to claim 1, wherein the exhaust pressure equalizing holes are located at the symmetry planes corresponding to the two sides of the test paper placement groove.
The microporous uniform pressure and anti-capillary fast sampling fluid detection device according to claim 1, characterized in that: the utility model further comprises a cover sheet; the cover sheet covers the working side of the carrier sheet; the top edge of the cover sheet and The top edges of the carrier sheet are aligned; the distance from the bottom edge to the top edge of the cover sheet is greater than the distance from the bottom edge to the top edge of the carrier sheet.
8. The microporous constant pressure anti-capillary fast sampling fluid detection device according to claim 8, wherein the cover sheet is made of a transparent material.
The microporous constant pressure anti-capillary fast sampling fluid detection device according to claim 8, wherein the cover sheet is provided with an observation window corresponding to the test paper placement groove.