CN215050253U - Nucleic acid detection consumable - Google Patents
Nucleic acid detection consumable Download PDFInfo
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- CN215050253U CN215050253U CN202120005438.4U CN202120005438U CN215050253U CN 215050253 U CN215050253 U CN 215050253U CN 202120005438 U CN202120005438 U CN 202120005438U CN 215050253 U CN215050253 U CN 215050253U
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 96
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- 238000001514 detection method Methods 0.000 title claims abstract description 62
- 238000006243 chemical reaction Methods 0.000 claims abstract description 68
- 238000007789 sealing Methods 0.000 claims abstract description 45
- 239000012528 membrane Substances 0.000 claims abstract description 28
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 6
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000001917 fluorescence detection Methods 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 229910003460 diamond Inorganic materials 0.000 description 2
- 239000010432 diamond Substances 0.000 description 2
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Abstract
The utility model belongs to the nucleic acid detection field discloses a totally enclosed formula nucleic acid detection consumptive material that heat transfer effect is good to solve among the prior art traditional nucleic acid detection consumptive material (PCR pipe) heat transfer effect poor, easily cause technical problem such as aerosol pollution. The nucleic acid detection consumable includes: the chip body is provided with a sample inlet, a sample channel and a reaction area which are sequentially communicated; a sealing membrane for sealing the sample channel and the reaction region; a sealing membrane for sealing the sample inlet. This nucleic acid detects consumptive material uses the sealed chip body of involution membrane, makes only separate through the involution membrane between heat source and the reaction liquid, and the involution membrane has advantages such as satisfying biocompatibility and coefficient of heat conductivity height, can show improvement thermal-insulated character, and simultaneously, the involution membrane is changeed and is destroyed, combines together with totally closed nucleic acid product detection device more easily, realizes no nucleic acid pollution's terminal method product analysis.
Description
Technical Field
The utility model relates to a nucleic acid detects the field, especially relates to a nucleic acid detects consumptive material.
Background
The nucleic acid detection technology is a technology for analyzing the existence and content of target sequence nucleic acid in an object to be detected, and is widely applied to various fields of clinical diagnosis, medical research, environmental monitoring, food safety, criminal investigation and the like.
The nucleic acid detection process generally comprises nucleic acid sample extraction, nucleic acid amplification and product analysis, taking the detection process of a novel coronavirus (SARS-COV-2) as an example, a respiratory tract sample is inactivated and pretreated, then nucleic acid extraction is carried out to obtain a nucleic acid amplification template, the nucleic acid template is amplified under a proper temperature condition by using an N gene primer, a 1ab gene primer and an amplification reagent in a targeted manner, the content index of the nucleic acid template is amplified to reach the level that a physical signal can be detected, and the detection of the nucleic acid can be realized.
Among them, nucleic acid amplification, which amplifies an extremely small amount of nucleic acid template to a detectable level, is a key to nucleic acid detection, and the core of nucleic acid amplification lies in the assurance of appropriate temperature/temperature cycling. The existing nucleic acid amplification is generally carried out in a PCR tube, and the temperature of reagents in the reaction process is indirectly controlled by heating the PCR tube through a metal base. However, when the PCR tube is used for amplification, heat transfer limiting factors such as low heat transfer coefficient of materials, large tube wall thickness and unfavorable influence of liquid distribution rule on uniform temperature distribution exist, so that the temperature control effect in the amplification process is obviously reduced, and the nucleic acid amplification efficiency is further reduced. Therefore, reagent consumables capable of improving heat conduction and temperature control effects are important for improving nucleic acid amplification efficiency.
In addition, the detection of the nucleic acid amplification product is divided into real-time detection and end-point detection, the real-time detection mainly uses a fluorescent dye/probe method, the liquid amount in a PCR tube cannot be too small when the PCR tube is used for carrying out fluorescence detection, or effective light spots cannot be formed, the reagent dosage is large, and the cost is high; end point detection typically involves electrophoresis, hybridization, mass spectrometry, and turbidimetry, but the need for uncapping is prone to nucleic acid aerosol contamination problems.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a reagent quantity is few, the good totally enclosed nucleic acid detection consumptive material of heat transfer effect to solve among the prior art traditional nucleic acid detection consumptive material (PCR pipe) heat transfer effect poor, easily cause technical problem such as aerosol pollution.
In order to achieve the above purpose, the purpose of the present invention is achieved by the following technical solutions:
there is provided a nucleic acid detection consumable comprising: the chip body is provided with a sample inlet, a sample channel and a reaction area which are sequentially communicated; a sealing membrane for sealing the sample channel and the reaction region; a sealing membrane for sealing the sample inlet.
According to the utility model discloses nucleic acid detects consumptive material, the closure membrane is the one-sided glued membrane that has the biocompatibility, is the one-sided glued membrane that the biocompatible material was handled for example, preferably pressure-sensitive glued membrane.
According to the utility model discloses nucleic acid detects consumptive material, the thickness of sealing membrane is 50-100 μm.
According to the utility model discloses nucleic acid detects consumptive material, the thickness of sealing membrane is 50-75 μm.
According to the utility model discloses nucleic acid detects consumptive material, the shape of reaction zone is rhombus, circular or oval.
According to the utility model discloses nucleic acid detects consumptive material, the total volume of sample passageway and reaction zone is 5-200 mu L.
According to the utility model discloses nucleic acid detects consumptive material, the total volume of sample passageway and reaction zone is 12.5-100 mu L.
According to the utility model discloses nucleic acid detects consumptive material, the volume of reaction zone is 3-60 mu L
According to the utility model discloses nucleic acid detects consumptive material, the total volume of sample passageway and reaction zone is 95-115% of the total volume of the reaction solution that awaits measuring.
According to the utility model discloses nucleic acid detects consumptive material, the volume of reaction zone is 30-60% of the total volume of the reaction solution that awaits measuring.
According to the utility model discloses nucleic acid detects consumptive material, still be provided with balanced passageway on the chip body, balanced passageway intercommunication the sample entry with the reaction zone.
According to the utility model discloses nucleic acid detects consumptive material, the quantity of balanced passageway is two, distribute in the both sides of sample passageway.
According to the utility model discloses nucleic acid detects consumptive material, still be provided with the locating hole on the chip body, the locating hole is located the first half of chip body, sample entry, sample passageway and reaction zone are located the lower half of chip body.
According to the utility model discloses nucleic acid detects consumptive material, sample entry, sample passageway and reaction zone are arranged from top to bottom along the vertical direction of chip body in proper order.
The beneficial effects of the utility model reside in that:
1. the sealing film is used for sealing the chip body, so that a heat source and reaction liquid are separated only through the sealing film, the sealing film has the advantages of biological compatibility, high heat conductivity coefficient and the like, the heat conductivity can be obviously improved, meanwhile, the sealing film is easier to damage and is easier to combine with a fully-closed nucleic acid product detection device, and the product analysis of a terminal method without nucleic acid pollution is realized.
2. The reaction zone is also matched with a balance channel structure, so that the stable position of the fluid in the reaction zone can be ensured.
Drawings
FIG. 1 is a schematic structural diagram of a consumable for nucleic acid detection according to an embodiment of the present invention;
FIG. 2 is a side exploded view of the consumable for nucleic acid detection according to the embodiment of the present invention.
In the figure: 1-a chip body; 2-sealing the membrane; 3-sealing film; 11-sample inlet; 12-a sample channel; 13-a reaction zone; 14-a balance channel; 15-positioning holes.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in FIG. 1 to FIG. 2, the utility model provides a consumable for nucleic acid detection, which comprises: the chip body 1 is provided with a sample inlet 11, a sample channel 12 and a reaction area 13 which are sequentially communicated; a sealing membrane 2 for sealing the sample channel 12 and the reaction region 13; and a sealing film 3 for sealing the sample inlet 11.
When preparing the nucleic acid detection consumptive material, realize semi-closed through sealed sample passageway 12 of closure membrane 2 and reaction zone 13, when using the nucleic acid detection consumptive material, enter into reaction zone 13 back through sample entry 11 and sample passageway 12 with reaction solution and realize totally closed through sealed sample entry 11 of sealing membrane 3, then place totally closed nucleic acid detection consumptive material in the heat source environment and heat and carry out the amplification reaction, at this moment, the heat source only separates through the closure membrane with reaction solution, heat transfer distance is low, can show improvement heat transfer effect. Meanwhile, the sealing membrane 2 is used for sealing the reaction area 13, and the sealing membrane 2 is easier to damage and is easier to be combined with a totally-closed nucleic acid product detection device, so that the product analysis of the end-point method without nucleic acid pollution is realized.
More specifically, when preparing the nucleic acid detection consumptive material, obtain the chip body through machine tooling, the chip body after the processing is accomplished needs to carry out the preliminary treatment, and the preliminary treatment step includes: firstly, deburring a chip body, then soaking and washing the chip body for 30min by using clear water, then ultrasonically bathing the chip body for 30min by using 100mM NaOH and 0.1% Sodium Dodecyl Sulfate (SDS), and finally washing the chip body by using deionized water; after the pretreatment step is finished, the sealing film is sealed in the sample channel and the reaction area on the chip body by using a hydraulic machine to prepare the nucleic acid detection consumable for standby.
When the nucleic acid detection consumable is used, reaction liquid needs to be added from a sample inlet through a liquid device/pipe, then the sealing film is pasted at the sample inlet to realize the full sealing of the nucleic acid detection consumable, then the chip body is swung or flicked to remove bubbles possibly appearing in a reaction area, and the nucleic acid detection consumable can be inserted into a temperature control/detection device to start reaction. Further, sample entry, sample passageway and reaction zone arrange from top to bottom in proper order along the vertical direction of chip body, when using, vertically place the nucleic acid detection consumptive material.
Furthermore, the sealing film 2 is a single-sided adhesive film with biocompatibility, so that the sealing film 2 can meet the requirement of the biocompatibility, has high heat conductivity coefficient and further provides a heat transfer effect. Further, the thickness of the sealing film 2 is 50 to 100. mu.m, preferably 50 to 75 μm.
Furthermore, the sealing film 2 and the sealing film 3 are both provided with release paper and can be cut into required specifications by using a cutting die.
The reaction zone 13 is in the shape of a diamond, a circle or an ellipse. The reaction region 13 of the nucleic acid detecting consumable is preferably diamond-shaped. The constructed rhombic reaction zone 13 is designed according to the light path requirement of fluorescence detection, and the liquid quantity requirement can be reduced as much as possible on the premise of meeting the requirement of fluorescence detection, so that the volume requirement of a reaction system is reduced, and the reagent cost is further reduced.
The nucleic acid detection consumable body is also provided with an equilibrium channel 14, and the equilibrium channel 14 is communicated with the sample inlet 11 and the reaction area 13. Thus, both ends of the equilibrium channel 14 and both ends of the sample channel 12 are communicated by the sample inlet 11 and the reaction region 13, respectively, wherein one end of the equilibrium channel 14 and one end of the sample channel 12 are communicated at the sample inlet 11, and the other end of the equilibrium channel 14 and the other end of the sample channel 12 are communicated at the reaction region 13. The balance channel 14 can ensure the stable position of the fluid in the reaction zone.
Further, the number of the balance channels 14 is two, and the balance channels are distributed on two sides of the sample channel 12.
The total volume of the nucleic acid detection consumable is 5-200 mu L, and preferably 12.5-100 mu L. It is worth to say that, the total volume of the nucleic acid detection consumable can correspond to the detection specification of the nucleic acid detection consumable, and different total volumes correspond to different detection specifications, i.e., the nucleic acid detection consumable can be respectively used for detecting reaction solutions with different volumes according to different total volumes. Further, the total volume of the nucleic acid detection consumables is 95-115% of the total volume of the reaction solution to be detected, and preferably 105-110%.
In some embodiments, the total volume of the nucleic acid detection consumables is the total volume of the sample channel 12 and the reaction region 13.
In some embodiments, the total volume of the nucleic acid detecting consumables is the total volume of the sample channel 12, the reaction region 13, and the equilibrium channel 14.
Further, the volume of the reaction zone is 3-60. mu.L. The volume of the reaction zone 13 is 30 to 60%, preferably 40 to 50%, of the total volume of the reaction solution to be measured.
For example: for a nucleic acid detection consumable of 12.5. mu.L format, the volume of the reaction region 13 is 3-6. mu.L, preferably 4-5. mu.L; for a 25. mu.L format nucleic acid detection consumable, the volume of the reaction zone 13 is 7.5-15. mu.L, preferably 9-12.5. mu.L; for a nucleic acid detection consumable of 50. mu.L format, the volume of the reaction region 13 is 15 to 30. mu.L, preferably 20 to 25. mu.L; for a 100. mu.L format nucleic acid detection consumable, the reaction region 13 has a volume of 30 to 60. mu.L, preferably 40 to 50. mu.L.
The nucleic acid detection consumable body is also provided with a positioning hole 15, the positioning hole 15 is positioned at the upper half part of the chip body, and the sample inlet 11, the sample channel 12, the reaction area 13 and the balance channel 14 are positioned at the lower half part of the chip body.
Example 1
The nucleic acid detection consumable of the present embodiment includes: chip body, sealing membrane and sealing membrane. The detection specification of the nucleic acid detection consumable is 25 muL, wherein the size of the chip body is as follows: the chip is 40mm multiplied by 15mm multiplied by 2mm, and the chip body is made of PP material; the sealing film is a 3M pressure-sensitive adhesive film with the thickness of 60 mu M; the sealing film is single-sided adhesive with release paper, and the size is 15mm multiplied by 7.5mm multiplied by 0.05 mm.
The chip body is provided with a sample inlet, a sample channel, a reaction area and a balance channel, wherein the diameter of the sample inlet is 4mm, the volume of the reaction area is 12.5 mu L, the total volume of the sample channel and the balance channel is 14 mu L, and the included angle of two top side surfaces parallel to a fluorescence light path and corresponding to the diamond structure of the reaction area is 45 degrees.
When the kit is used, 25 mu L of reaction liquid is added from a sample inlet through a pipette, and the sealing film is attached to the sample inlet, so that the complete sealing of the nucleic acid detection consumable is realized. Then the upper part of the nucleic acid detection consumable is pinched by hand, and the bottom of the consumable is flicked by fingers to remove air bubbles possibly existing in the reaction area. And finally, inserting the consumable into the temperature control equipment to start reaction.
Use and be based on the utility model discloses a nucleic acid detects consumptive material and conventional nucleic acid detects consumptive material and compares:
utilize conventional PCR amplification consumptive material to carry out nucleic acid testing, utilize simultaneously the utility model provides a nucleic acid testing consumptive material is according to the embodiment the mode is operated, and the contrast result is shown in table 1:
TABLE 1
Can know in table 1, compare with conventional PCR amplification consumptive material (PCR pipe), the utility model provides a nucleic acid detects consumptive material because of the structural feature bubble gets rid of more conveniently and there is certain advantage in the application of sample process, and the good temperature raising and lowering time that can show and shorten reaction liquid of heat transfer effect simultaneously, thereby can the greatly reduced reagent liquid measure requirement reduce cost to the reaction zone of fluorescence light path design, still be expected to provide new nucleic acid product analysis pollution-free solution with anti-pollution device cooperation in addition.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (10)
1. A nucleic acid detection consumable, comprising:
the chip body is provided with a sample inlet, a sample channel and a reaction area which are sequentially communicated;
a sealing membrane for sealing the sample channel and the reaction region;
a sealing membrane for sealing the sample inlet.
2. The nucleic acid detection consumable of claim 1, wherein the sealing membrane is a biocompatible single-sided adhesive membrane.
3. The nucleic acid detection consumable of claim 1, wherein the sealing membrane has a thickness of 50-100 μm.
4. The nucleic acid detection consumable of claim 1, wherein the reaction zone is diamond-shaped, circular, or oval in shape.
5. The nucleic acid detection consumable of claim 1, wherein the total volume of the sample channel and the reaction region is 5-200 μ L, and the volume of the reaction region is 3-60 μ L.
6. The nucleic acid detection consumable of claim 1, wherein the total volume of the sample channel and the reaction area is 95-115% of the total volume of the reaction solution to be detected; the volume of the reaction area is 30-60% of the total volume of the reaction liquid to be measured.
7. The consumable for nucleic acid detection according to claim 1, wherein the chip body is further provided with an equilibrium channel, and the equilibrium channel is communicated with the sample inlet and the reaction region.
8. The nucleic acid detection consumable of claim 7, wherein the number of the equilibration channels is two and the equilibration channels are distributed on both sides of the sample channel.
9. The consumable for nucleic acid detection according to claim 1, wherein the chip body is further provided with a positioning hole, the positioning hole is located on the upper half part of the chip body, and the sample inlet, the sample channel and the reaction region are located on the lower half part of the chip body.
10. The nucleic acid detection consumable of claim 1, wherein the sample inlet, the sample channel, and the reaction region are sequentially arranged from top to bottom along a vertical direction of the chip body.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202120005438.4U CN215050253U (en) | 2021-01-04 | 2021-01-04 | Nucleic acid detection consumable |
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| CN202120005438.4U CN215050253U (en) | 2021-01-04 | 2021-01-04 | Nucleic acid detection consumable |
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| CN215050253U true CN215050253U (en) | 2021-12-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115747037A (en) * | 2023-01-09 | 2023-03-07 | 深圳市科瑞达生物技术有限公司 | Nucleic acid analysis card and method of use thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115747037A (en) * | 2023-01-09 | 2023-03-07 | 深圳市科瑞达生物技术有限公司 | Nucleic acid analysis card and method of use thereof |
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Address after: 523808 No.1, Gongye North Road, Songshanhu Park, Dongguan City, Guangdong Province Patentee after: Guangdong Dongyangguang Pharmaceutical Co.,Ltd. Address before: 523808 No.1, Gongye North Road, Songshanhu Park, Dongguan City, Guangdong Province Patentee before: SUNSHINE LAKE PHARMA Co.,Ltd. |
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