WO2021041260A1 - Enzymatic rna capping method - Google Patents

Enzymatic rna capping method Download PDF

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Publication number
WO2021041260A1
WO2021041260A1 PCT/US2020/047521 US2020047521W WO2021041260A1 WO 2021041260 A1 WO2021041260 A1 WO 2021041260A1 US 2020047521 W US2020047521 W US 2020047521W WO 2021041260 A1 WO2021041260 A1 WO 2021041260A1
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WIPO (PCT)
Prior art keywords
rna
seq
capping
cap
enzyme
Prior art date
Application number
PCT/US2020/047521
Other languages
English (en)
French (fr)
Inventor
G. Brett Robb
Siu-Hong Chan
Bijoyita Roy
Original Assignee
New England Biolabs, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by New England Biolabs, Inc. filed Critical New England Biolabs, Inc.
Priority to AU2020336278A priority Critical patent/AU2020336278A1/en
Priority to JP2022512342A priority patent/JP2022544712A/ja
Priority to KR1020227009727A priority patent/KR20220046693A/ko
Priority to BR112022003517A priority patent/BR112022003517A2/pt
Priority to PCT/US2020/047533 priority patent/WO2021041267A1/en
Priority to CA3147797A priority patent/CA3147797A1/en
Priority to US17/001,236 priority patent/US20210054016A1/en
Publication of WO2021041260A1 publication Critical patent/WO2021041260A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01056—Methyltransferases (2.1.1) mRNA (guanine-N7-)-methyltransferase (2.1.1.56)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02003—Uridine phosphorylase (2.4.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07045—Guanosine-triphosphate guanylyltransferase (2.7.7.45)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
PCT/US2020/047521 2019-08-23 2020-08-21 Enzymatic rna capping method WO2021041260A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2020336278A AU2020336278A1 (en) 2019-08-23 2020-08-21 Enzymatic RNA capping method
JP2022512342A JP2022544712A (ja) 2019-08-23 2020-08-21 酵素的ｒｎａキャッピング法
KR1020227009727A KR20220046693A (ko) 2019-08-23 2020-08-21 효소학적 rna 캡핑 방법
BR112022003517A BR112022003517A2 (pt) 2019-08-23 2020-08-21 Método de capeamento de rna enzimático
PCT/US2020/047533 WO2021041267A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method
CA3147797A CA3147797A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method
US17/001,236 US20210054016A1 (en) 2019-08-23 2020-08-24 Enzymatic RNA Capping Method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962890821P 2019-08-23 2019-08-23
US62/890,821 2019-08-23

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2020/047533 Continuation WO2021041267A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US17/001,236 Continuation US20210054016A1 (en) 2019-08-23 2020-08-24 Enzymatic RNA Capping Method

Publications (1)

Publication Number Publication Date
WO2021041260A1 true WO2021041260A1 (en) 2021-03-04

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PCT/US2020/047533 WO2021041267A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method
PCT/US2020/047521 WO2021041260A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method

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PCT/US2020/047533 WO2021041267A1 (en) 2019-08-23 2020-08-21 Enzymatic rna capping method

Country Status (3)

Country Link
EP (1) EP4017995A1 (zh)
CN (1) CN112714795A (zh)
WO (2) WO2021041267A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022164428A1 (en) * 2021-01-27 2022-08-04 New England Biolabs, Inc. Faustovirus capping enzyme, mrna capping enzyme compositions, methods and kits
US11725196B2 (en) 2021-01-27 2023-08-15 New England Biolabs, Inc. FCE mRNA capping enzyme compositions, methods and kits

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117069850A (zh) * 2021-12-20 2023-11-17 南京诺唯赞检测技术有限公司 一种vce的单克隆抗体及其制备方法
WO2023115495A1 (zh) * 2021-12-23 2023-06-29 北京毅新博创生物科技有限公司 质谱法检测mRNA加帽效率的方法
CN114369587B (zh) * 2021-12-25 2024-03-15 苏州瀚源新酶生物科技有限公司 牛痘病毒加帽酶突变体、重组载体、重组工程菌及其应用
CN114438084A (zh) * 2022-01-29 2022-05-06 上海兆维科技发展有限公司 一种转录反应液、锁核酸修饰的rna的制备方法以及突变的t7rna聚合酶的应用
CN114574466B (zh) * 2022-05-09 2022-08-02 翌圣生物科技(上海)股份有限公司 一种嵌合加帽酶及其制备方法与应用
CN114941018B (zh) * 2022-06-28 2023-09-22 翌圣生物科技(上海)股份有限公司 cap1帽类似物的合成方法
CN116836974B (zh) * 2023-09-01 2024-02-20 苏州近岸蛋白质科技股份有限公司 一种体外合成加帽mRNA的方法

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WO2015085142A1 (en) 2013-12-05 2015-06-11 New England Biolabs, Inc. Compositions and methods for capping rna
US20150167017A1 (en) 2013-12-13 2015-06-18 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
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US20180237817A1 (en) * 2015-05-29 2018-08-23 Curevac Ag Method for adding cap structures to rna using immobilized enzymes
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WO2018236617A1 (en) 2017-06-21 2018-12-27 New England Biolabs, Inc. USE OF THERMOSTABLE POLYMERAS RNA TO PRODUCE RNA WITH REDUCED IMMUNOGENICITY
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022164428A1 (en) * 2021-01-27 2022-08-04 New England Biolabs, Inc. Faustovirus capping enzyme, mrna capping enzyme compositions, methods and kits
US11725196B2 (en) 2021-01-27 2023-08-15 New England Biolabs, Inc. FCE mRNA capping enzyme compositions, methods and kits

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Publication number Publication date
EP4017995A1 (en) 2022-06-29
CN112714795A (zh) 2021-04-27
WO2021041267A1 (en) 2021-03-04

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