WO2021036121A1

WO2021036121A1 - E-liquid

Info

Publication number: WO2021036121A1
Application number: PCT/CN2019/127156
Authority: WO
Inventors: 许静; 姜兴涛; 熊赞华; 李馨铎
Original assignee: 深圳雾芯科技有限公司
Priority date: 2019-08-30
Filing date: 2019-12-20
Publication date: 2021-03-04
Also published as: CN110353298A

Abstract

An e-liquid, comprising naringenin, wherein the content of naringenin in the e-liquid is 0.09% to 10% the total weight of the e-liquid. After the e-liquid is atomized, the naringenin contained therein may be atomized together with the e-liquid and absorbed by a human body, thereby achieving anti-inflammatory, cough-relieving and phlegm-eliminating effects, and inhibiting oral and respiratory bacteria. Moreover, the taste of the e-liquid after atomization is made more comfortable and milder, the aroma of the cigarette is enriched, and the quality of smoking is improved.

Description

Electronic cigarette liquid

Technical field

This application relates to the field of electronic aerosols or electronic atomization devices, and in particular to an electronic cigarette liquid.

Background technique

Traditional tobacco smoking has many possible side effects, such as cardiovascular disease, cancer, chronic obstructive pulmonary disease, etc., which seriously endanger human health. Among them, smoking has the most direct effect on the respiratory system. The harmful substances released by traditional tobacco burning at high temperatures can reduce the defense function of the respiratory tract, proliferate goblet cells, increase mucus secretion, abnormal cilia activity, and reduce mucus excretion. This leads to various respiratory diseases such as pneumonia and chronic obstructive pulmonary disease.

The low-temperature atomization of electronic cigarette oil is not easy to produce harmful substances such as tar, suspended particulates and carbon monoxide that are harmful to human health. The harm to users and others is far less than the harm of traditional cigarettes to the human body.

Therefore, how to further generate aerosol for user inhalation that reduces harmful effects on the human body and improves the beneficial effects on the human body through low-temperature atomization is a research direction that needs to be improved in electronic cigarette products.

Summary of the invention

The present application provides an e-cigarette liquid containing naringenin. The e-cigarette liquid is generated by an atomization device for the user to inhale a certain amount of naringenin in the aerosol that can be absorbed by the human body. Health effects. When the user is smoking the electronic cigarette liquid, the health effects of clearing heat and moisturizing the lungs, relieving cough and eliminating phlegm can be obtained at the same time.

According to one aspect of the application, the application provides an e-cigarette liquid comprising naringenin, wherein the content of the naringenin in the e-cigarette liquid is about 0.09 based on the total weight of the e-cigarette liquid. % To about 10%.

According to another aspect of the present application, the present application provides an atomization device, which includes the above-mentioned e-cigarette liquid. The electronic cigarette liquid of the present application can play a role in inhibiting oral inflammation, coughing or preventing the growth of bacteria.

The additional aspects and advantages of the embodiments of the present application will be partially described, shown, or explained through the implementation of the embodiments of the present application in the subsequent description.

Description of the drawings

Fig. 1A is a chromatogram of the naringenin content of the electronic cigarette liquid according to an embodiment of the present application.

FIG. 1B is a chromatogram of the naringenin content of the aerosol generated by the electronic cigarette liquid according to the embodiment of the present application.

detailed description

The embodiments of this application will be described in detail below. The embodiments of this application should not be construed as limitations on this application.

In addition, sometimes amounts, ratios, and other numerical values are presented in range format herein. It should be understood that such a range format is for convenience and brevity, and should be understood flexibly, not only includes the values explicitly designated as range limits, but also includes all individual values or sub-ranges within the stated range, as if clearly Specify each value and sub-range in general.

As used herein, the terms "substantially", "substantially", "substantially", "substantially" and "about" are used to describe and illustrate small changes. When used in conjunction with an event or situation, the term can refer to an example in which the event or situation occurs precisely and an example in which the event or situation occurs very closely. For example, when used in conjunction with a value, the term can refer to a range of variation less than or equal to ±10% of the stated value, such as less than or equal to ±5%, less than or equal to ±4%, less than or equal to ±3%, Less than or equal to ±2%, less than or equal to ±1%, less than or equal to ±0.5%, less than or equal to ±0.1%, or less than or equal to ±0.05%. For example, if the difference between two values is less than or equal to ±10% of the average value of the value (for example, less than or equal to ±5%, less than or equal to ±4%, less than or equal to ±3%, less than Or equal to ±2%, less than or equal to ±1%, less than or equal to ±0.5%, less than or equal to ±0.1%, or less than or equal to ±0.05%), then the two values can be considered "substantially" the same.

In the detailed description and claims, a list of items connected by the terms "at least one of", "at least one of", "at least one of" or other similar terms may mean the listed items Any combination of. For example, if items A and B are listed, then the phrase "at least one of A and B" means only A; only B; or A and B. In another example, if items A, B, and C are listed, then the phrase "at least one of A, B, and C" means only A; or only B; only C; A and B (excluding C); A and C (exclude B); B and C (exclude A); or all of A, B, and C. Project A can contain a single element or multiple elements. Project B can contain a single element or multiple elements. Project C can contain a single element or multiple elements.

In this context, the term "aerosol for user inhalation" may include, but is not limited to, aerosols, suspended liquids, low-temperature vapors, and volatile gases.

The present application provides an electronic cigarette liquid containing naringenin, which can have various beneficial effects such as anti-inflammatory, relieving cough, reducing phlegm, and inhibiting bacteria while inhaling the atomized electronic cigarette liquid.

According to one aspect of the present application, the present application provides an electronic cigarette liquid, which contains naringenin.

Naringenin has antibacterial, anti-inflammatory, scavenging free radicals, anti-oxidation, anti-tussive and expectorant, lowering blood fat, anti-cancer, anti-tumor, antispasmodic and choleretic, preventing and treating liver disease, inhibiting platelet coagulation, anti-atherosclerosis, etc. . In some embodiments, when the e-cigarette liquid forms an aerosol through the atomizing device, the naringenin contained in it can be mixed into the aerosol through the atomization, and when the user inhales the aerosol, it can directly absorb the grapefruit peel. Therefore, it can prevent the inflammation, cough, phlegm and other reactions that may be caused by smoking. At the same time, because naringenin has a certain antibacterial effect, the e-cigarette liquid with naringenin can also achieve the effect of inhibiting bacteria in the oral cavity and respiratory tract.

In some embodiments, the content of the naringenin in the e-cigarette liquid, based on the total weight of the e-cigarette liquid, is approximately, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.15%, 0.5%, 1.0%, 1.5%, 5.0%, 10.0%, 15% or a range composed of any two of these parts. In some embodiments, the content of the naringenin in the e-cigarette liquid is about 0.09% to about 10% based on the total weight of the e-cigarette liquid, and the naringenin can make the e-cigarette liquid atomized. The taste is more comfortable and milder, enriching the aroma of the smoke and improving the quality of the food.

The e-cigarette liquid also includes a base solvent, wherein the base solvent includes propylene glycol and glycerol (glycerin). In some embodiments, based on the total weight of the e-liquid, the content of propylene glycol in the e-liquid is approximately, for example, 15%, 20%, 25%, 30%, 35%, 40%, 50%, 60%, 70% or any two of these weight percentages; and the content of glycerol in the e-cigarette liquid is roughly, for example, 30%, 35%, 40%, 45%, 50%, 55% , 60%, 65%, 70% or any two of these weight percentages. In some embodiments, the content of the propylene glycol in the e-cigarette liquid is about 20% to about 60%, and the content of the glycerol in the e-cigarette liquid is about 40% to about 70%, so that the electronic cigarette The smoke liquid achieves the best atomization effect and improves the user experience.

Those skilled in the art should understand that the basic solvent can be any solvent known in the art, such as deionized water, propylene glycol, and glycerol, according to specific needs, without being limited.

In some embodiments, the electronic cigarette liquid further comprises a cooling agent, wherein the content of the cooling agent in the electronic cigarette liquid is approximately, for example, 0.1%, 0.2%, 0.3%, 0.5%, 1.0% , 1.5%, 2.0%, 2.5%, 5% or any two of these weight percentages.

Cooling agents are additives, which are chemicals that can give users a cooling effect when the product is in use and are not very irritating. In some embodiments, the cooling agent includes selected from the group consisting of menthol, menthone, isomenthone, menthyl lactate, WS-23 (N,2,3-trimethyl-2-isopropylbutanamide) , WS-3(N-ethyl-p-menthyl-3-carboxamide), WS-5(N-(ethoxycarbonylmethyl)-p-alkane-3-carboxamide), WS-12(N-( 4-methoxyphenyl)-p-menthyl-3-carboxamide), menthyl acetate and combinations thereof.

In some embodiments, the e-cigarette liquid further includes flavor components; wherein the flavor components are selected from the group consisting of ursolic acid, mogroside, synephrine, glycyrrhizin, and combinations thereof.

Ursolic acid has the effects of clearing the lungs and relieving cough, lowering the adverse effects and relieving vomiting, and is used to treat lung-heat cough, shortness of breath, gastric heat, vomiting, and thirst. Momordica grosvenori has the effects of clearing heat and moisturizing the lungs, stimulating the sound of the throat, and smoothing the bowels of the intestines to treat pulmonary heat and dry cough, sore throat, aphonia, intestinal dryness and constipation. Synephrine can dispel the wind and regulate the qi, warm the stomach, promote appetite and accelerate metabolism, and is also a fragrant antiphlegm agent. Glycyrrhizin has the effects of invigorating the spleen and qi, detoxifying heat, eliminating phlegm and relieving cough, relieving emergency and relieving pain, and reconciling various drugs. It can be used to treat weakness of the spleen and stomach, fatigue, fatigue, cough and excessive sputum. The electronic cigarette liquid of the present application can further inhibit the inflammation, cough, and sputum in the oral cavity and respiratory tract through the added flavor components, and achieve the effects of antibacterial and oral cleaning during use.

In some embodiments, the content of the flavor component in the e-cigarette liquid, based on the total weight of the e-cigarette liquid, is approximately, for example, 0.001%, 0.005%, 0.01%, 0.05%, 0.06%, 0.07 %, 0.08%, 0.09%, 0.1%, 0.15%, 0.5%, 1.0%, 1.5%, 5.0%, 10.0%, 15% or any two of these parts. In some embodiments, the content of the flavor component in the e-cigarette liquid is about 0.001% to about 10% based on the total weight of the e-cigarette liquid, and the flavor component can enable the e-cigarette liquid to be generated for The mouthfeel of the aerosol inhaled by the user is more mellow and delicate, which enriches the fragrance of the aerosol, thereby enhancing the user experience.

In some embodiments, the e-cigarette liquid further includes nicotine salt. In some embodiments, based on the total weight of the e-cigarette liquid, the content of the nicotine salt in the e-cigarette liquid is approximately, for example, 0.001%, 0.01%, 0.1%, 1.0% , 1.5%, 2.0%, 2.5%, 5%, 7.5%, 10% or any two of these weight percentages. In some embodiments, the content of the nicotine salt in the e-cigarette liquid is about 0.001% to about 10.0% based on the total weight of the e-cigarette liquid.

It should be understood that those skilled in the art can select any known nicotine salt in the art to add to the e-cigarette liquid according to actual needs without limitation. In some embodiments, the nicotine salt comprises benzoate.

In some embodiments, the e-cigarette liquid further includes nicotine. In some embodiments, based on the total weight of the e-cigarette liquid, the content of the nicotine in the e-cigarette liquid is approximately, for example, 0.001%, 0.01%, 0.1%, 0.2%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 5%, or any two of these weight percentages. In some embodiments, the content of the nicotine in the e-cigarette liquid is about 0.001% to about 5.0% based on the total weight of the e-cigarette liquid.

In some embodiments, the above-mentioned e-cigarette liquid can further include flavor.

In some embodiments, the flavor includes: at least one of citronellol, vanillin, limonene, citronone, and ethyl maltol.

Citronellol has a special aroma like fresh rose and has a bitter taste. Adding to the e-cigarette liquid can make the aroma in the generated aerosol more fresh. Vanillin has a pure vanilla flavor, a stable, pure, and odor-free aroma. Adding to the electronic cigarette liquid can make the generated aerosol more pure and delicate. Limonene has a lemon-like scent, and when added to the e-cigarette liquid, the aerosol generated can be fuller and sweeter. Pomelo ketone has a typical citrus and grapefruit aroma. Adding to the e-cigarette liquid can enrich the aroma in the aerosol generated, thereby reducing irritation. Ethyl maltol has a caramel aroma and a fruity taste. Adding to the e-cigarette liquid can improve the aroma in the aerosol that it generates, and at the same time can make the aroma more full.

In some embodiments, the content of the flavor in the e-cigarette liquid, based on the total weight of the e-cigarette liquid, is approximately, for example, 0.01%, 0.1%, 0.25%, 0.5%, 1.0%, 2.0% , 3.0%, 4.0%, 5.0%, 6.0%, 8.0%, 10% or any two of these parts. In some embodiments, the content of the flavor in the e-cigarette liquid is about 0.5% to about 6% based on the total weight of the e-cigarette liquid.

According to some embodiments of the present application, the present application provides a method for preparing an e-cigarette liquid, which includes the following steps: dissolve a certain amount of naringenin in a basic solvent, and then add flavor components and nicotine salts, and then mix them evenly To obtain e-cigarette liquid.

Those skilled in the art should understand that the electronic cigarette liquid of the embodiments of the present application can be manufactured by any suitable known method according to actual preparation requirements, without limitation.

According to another aspect of the present application, the present application provides an atomization device, wherein the atomization device includes the electronic cigarette liquid in the above embodiment. The electronic cigarette liquid can be atomized by the atomizing device to generate aerosol for inhalation by the user.

In some embodiments, the atomization device includes an electronic cigarette, an ultrasonic atomizer, or a mesh atomizer.

Example

In order to facilitate a better understanding of this application, the following examples are used to illustrate. These examples belong to the scope of protection of this application, but do not limit the scope of protection of this application.

Example 1

Add 3 grams of naringenin to 47 grams of propylene glycol, and then add 50 grams of glycerin to mix, and stir evenly to obtain an electronic cigarette liquid.

Example 2

Add 3 grams of naringenin to 47 grams of propylene glycol, then add 3 grams of ursolic acid and 2 grams of synephrine and mix well, then add 45 grams of glycerin to mix, and stir well to obtain an electronic cigarette liquid.

Example 3

Add 3 grams of naringenin to 47 grams of propylene glycol, then add 1 gram of mogroside and 3 grams of glycyrrhizin and mix well, then add 45 grams of glycerin, mix well, and mix well to obtain an electronic cigarette liquid.

Example 4

Add 3 grams of naringenin to 47 grams of propylene glycol, then add 2 grams of ursolic acid, 1 gram of mogroside, 1 gram of synephrine and 1 gram of glycyrrhizin and mix well, then add 45 grams of glycerin to mix, and mix well. , Get the e-cigarette liquid.

Example 5

Add 3 grams of naringenin to 37 grams of propylene glycol, and then add 2 grams of nicotine salt, 2.06 grams of limonene, 0.34 grams of vanillin, 0.08 grams of ethyl maltol, 0.01 grams of naringenone, and 55.6 grams of glycerol to mix, and stir. Evenly, an electronic cigarette liquid is obtained.

Example 6

Add 1 g of naringenin to 43 g of propylene glycol, then add 3 g of ursolic acid and 2 g of synephrine and mix well, then add 3 g of nicotine salt, 1.43 g of limonene, 0.47 g of vanillin, and 0.1 g of ethyl malt. Phenol, 0.04 g round grape ketone and 46 g glycerol are mixed and stirred evenly to obtain an electronic cigarette liquid.

Example 7

Add 2 grams of naringenin to 34 grams of propylene glycol, then add 1 gram of mogroside and 3 grams of glycyrrhizin and mix well, then add 5 grams of nicotine salt, 2.98 grams of limonene, 0.45 grams of vanillin, and 0.15 grams of ethyl maltol. , 0.07 g of round grapefruit ketone and 51.35 g of glycerin are mixed and stirred evenly to obtain an electronic cigarette liquid.

Example 8

Add 1g of naringenin to 38g of propylene glycol, then add 2g of ursolic acid, 1g of mogroside, 1g of synephrine and 1g of glycyrrhizin and mix well, then add 5g of nicotine salt, 1.63g of limonene, 0.43 grams of vanillin, 0.01 grams of citronellol, 0.11 grams of ethyl maltol, 0.02 grams of citronone, and 48.8 grams of glycerol were mixed and stirred evenly to obtain an electronic cigarette liquid.

Subsequently, the e-cigarette liquid of Example 1 and its atomized aerosol were measured for naringenin content, and the e-cigarette liquids of Examples 1-8 were subjected to a bacterial inhibition test and a taste score test. To further illustrate, the content of naringenin in the aerosol of the e-cigarette liquid of the present application, the antibacterial effect and the improvement of the user experience during the use process.

Naringenin content test method;

Sample preparation: The e-cigarette liquid of Example 1 was used to generate the aerosol for inhalation by the user using the following suction method: the atomization device was an electronic cigarette, the suction time was 3.0 seconds ± 0.1 seconds, and the interval between puffs It is 30.0 seconds ± 0.5 seconds, the number of puffs is 50 times, and the volume of each puff is 55 mL ± 0.3 mL. A Cambridge filter was used to collect the aerosol, and 10 mL of methanol was used to immerse the Cambridge filter. After 30 minutes of ultrasonic extraction, a flue gas extraction solution was obtained.

Liquid chromatography test: Ultra-high performance liquid chromatography (UPLC) (model: Waters ACQUITY)

The HSS T3 C ₁₈ chromatographic column (2.1mm×100mm, 1.7μm) is used to measure the naringenin content of the e-cigarette liquid and its aerosol extraction solution. The liquid chromatography conditions are as follows: the mobile phase is acetonitrile-0.1% formic acid solution ( 30:70, v/v), isocratic elution, flow rate of 0.3mL/min, column temperature of 35°C, injection volume of 5μL, and detection wavelength of 276nm.

Figures 1A and 1B are respectively chromatograms of the naringenin content measured by UPLC of the e-cigarette liquid and the aerosol generated therefrom according to an embodiment of the present application.

As shown in Figures 1A and 1B, naringenin is present in the aerosol of the e-cigarette liquid of the present application, and the composition of naringenin in the aerosol is basically the same as the composition of the e-cigarette liquid. Therefore, naringenin can be effectively absorbed by the human body through the aerosol after the electronic cigarette, and then achieve the effects of anti-inflammatory, anti-cough, phlegm and inhibit bacteria.

Bacterial inhibition test method;

Sample preparation: The CORESTA (Cooperation Centre for Scientific Research Relative to Tobacco) standard (test document: ISO 20768:2018) is used to atomize the sample to generate aerosol through an atomizing device (electronic cigarette), and a Cambridge filter is used to collect the aerosol. Its aerosol absorption weight is 0.716g. Then put the Cambridge filter into a small beaker, then add 7.16 mL of 50% ethanol, and use ultrasonic extraction for 30 minutes to prepare an extraction solution with a concentration of 100 mg/mL. The extraction solution was diluted to obtain a test solution with a series of gradient concentrations (50, 25, 12.5, 6.25, 3.125, 1.562, 0.781 mg/mL).

Preparation of bacterial suspension: Take out the preserved strains: Streptococcus mutans (ATCC 25175) and Porphyromonas gingivalis (ATCC 33277) from the environment of -80°C, pick an appropriate amount of colonies from them, place them on the plate, and place them on the plate at 37°C. Incubate at ℃ for 36 h to activate. Then pick an appropriate amount of colonies from the plate and place them in another plate, incubate at 37° C. for 36 h, and repeat this step twice.

Use an inoculating loop to pick an appropriate amount of bacteria in a centrifuge tube, shake well, and dilute to obtain a bacterial suspension of 105 to 106 CFU/mL.

Preparation of the medicated plate: Take 1 mL of test solution of different concentrations and add it to the petri dish, then add 9 mL of the culture medium to the petri dish, and shake it well. Prepare a series of gradient concentration (10, 5, 2.5, 1.25, 0.625, 0.3125, 0.1562, 0.0781 mg/mL) drug plates, the sample solvent is a negative control, after pouring, place the petri dish horizontally to solidify the medium for use.

Bacterial inhibition assay: Take 100μL of bacterial suspension and spread it evenly on the medicated plate. Invert the culture at 37°C for 24 hours, observe the growth of the test bacteria, and record the minimum sample concentration that completely inhibits the growth of the bacteria as the Minimum Inhibitory Concentration (MIC).

The test results of the e-cigarette liquids of Examples 1-8 through the bacterial inhibition test are shown in Table 1 below.

Table 1

It can be seen from the data in Table 1 that the e-cigarette liquids in Examples 1-8 of the present application can effectively inhibit the growth of bacteria, so as to achieve the effects of cleaning the oral cavity and preventing dental caries.

Taste score test method;

Statistical method: 20 reviewers were asked to rate the aerosol experience generated by the e-cigarette liquids of the foregoing Examples 1-8 according to the evaluation indicators in Table 2 below.

Table 2

The test results of the e-cigarette liquids of Examples 1-8 through the mouthfeel scoring test are shown in Table 3 below.

table 3

From the data in Table 3, it can be seen that the aerosol formed by the e-cigarette liquids of Examples 1-8 of the present application after atomization for users to inhale has a good user experience in terms of mouthfeel and taste. . In addition, by comparing the examples, it can be seen that adding flavor components to the electronic cigarette liquid can further enhance the taste and mouthfeel of the aerosol generated by the electronic cigarette liquid.

It should be understood from the foregoing embodiments of this application that this application provides an e-cigarette liquid containing naringenin. The e-cigarette liquid is generated by an atomizing device for the user to inhale a certain amount of Naringenin is absorbed by the human body to reduce inflammation, relieve cough and expectorant. At the same time, the e-cigarette liquid has further antibacterial effects. The electronic cigarette liquid of the present application can be atomized by the atomization device, and the mouthfeel after atomization is more comfortable and milder, enriching the aroma of the cigarette, and improving the quality of smoking food

Although the application has been described and illustrated with reference to the specific embodiments of the application, these descriptions and illustrations do not limit the application. Those skilled in the art can clearly understand that without departing from the true spirit and scope of the application as defined by the appended claims, various changes can be made to make specific situations, materials, material compositions, and materials The method or process is suitable for the goal, spirit and scope of this application. All such modifications are intended to be within the scope of the appended claims. Although the methods disclosed herein have been described with reference to specific operations performed in a specific order, it should be understood that these operations can be combined, subdivided, or reordered to form equivalent methods without departing from the teachings of this application. Therefore, unless specifically indicated herein, the order and grouping of operations is not a limitation of the present application.

The foregoing summarizes the features of several embodiments and details of this application. The embodiments described in this application can be easily used as a basis for designing or modifying other processes and structures for performing the same or similar purpose and/or obtaining the same or similar advantages of the embodiments introduced herein. Such equivalent constructions do not depart from the spirit and scope of the present application, and various changes, substitutions and changes can be made without departing from the spirit and scope of the present application.

Claims

An electronic cigarette liquid comprising naringenin, wherein the content of the naringenin in the electronic cigarette liquid is 0.09% to 10% based on the total weight of the electronic cigarette liquid.
The electronic cigarette liquid according to claim 1, further comprising nicotine salt, and the content of the nicotine salt in the electronic cigarette liquid is 0.001% to 10% based on the total weight of the electronic cigarette liquid.
The electronic cigarette liquid according to claim 1, further comprising nicotine, and the content of the nicotine in the electronic cigarette liquid is 0.001% to 5% based on the total weight of the electronic cigarette liquid.
The electronic cigarette liquid according to any one of claims 1 to 3, further comprising a flavor.
The electronic cigarette liquid according to claim 4, wherein the flavor includes at least one of citronellol, vanillin, limonene, citrone, and ethyl maltol.
The electronic cigarette liquid according to claim 5, wherein the content of the flavor in the electronic cigarette liquid is 0.5% to 6% based on the total weight of the electronic cigarette liquid.
The electronic cigarette liquid according to any one of claims 1 to 3, further comprising a flavor component; wherein the flavor component is selected from the group consisting of ursolic acid, mogroside, synephrine, glycyrrhizin, and combinations thereof 'S group.
The electronic cigarette liquid according to claim 7, wherein the content of the flavor component in the electronic cigarette liquid is 0.001% to 10% based on the total weight of the electronic cigarette liquid.
An atomizing device comprising the electronic cigarette liquid according to any one of claims 1 to 8.
The atomization device according to claim 9, wherein the atomization device comprises an electronic cigarette, an ultrasonic atomizer or a mesh atomizer.