WO2021020759A1

WO2021020759A1 - Restrainer for tail vein injection or blood collection of small animal

Info

Publication number: WO2021020759A1
Application number: PCT/KR2020/008909
Authority: WO
Inventors: 박성걸; 남유리; 박소라; 임창묵
Original assignee: 박성걸
Priority date: 2019-07-30
Filing date: 2020-07-08
Publication date: 2021-02-04
Also published as: KR102038991B1

Abstract

The present invention relates to a restrainer for tail vein injection or blood collection of a small animal, which fixes a laboratory animal used for various experiments, especially a laboratory rat, and then facilitates blood collection from a tail portion thereof. By the restrainer, after a laboratory animal is arranged on a stable seat plate (100), a tail portion of the laboratory animal can be held easily and separately for blood vessel harvesting of or drug injection into a tail vein or a tail artery thereof, etc. Particularly, by the restrainer, a laboratory animal can be held very quickly and accurately, and also a tail portion thereof can be easily held in a state where the tail portion is bent about 90 degrees. Therefore, the restrainer can improve repetitive reproducibility and accuracy at the time of an experimenter's experiment.

Description

Small animal coarse vein administration or blood collection correction device

The present invention relates to a small-animal microvenous administration or blood collection correction device that assists the blood collection from the tail portion after fixing an experimental animal used for various experiments, particularly an experimental rat, and fixing the body of the experimental animal, and It relates to a small animal coarse vein administration or blood collection correction device that can be used for selectively inserting and collecting blood into a vein or artery located at.

In general, intravenous injection has many advantages over other routes. Since the drug reaches the necessary tissues of the body through the heart within a few seconds, the medicinal effect appears quickly, and the reaction also appears clearly. It is used to supply water, salt, and other necessary electrolytes when it is not possible to administer enough water orally, or when injecting drugs such as antidote into the blood or expecting rapid efficacy, including blood supply after bleeding.

Due to this characteristic, a newly developed drug or contrast agent is injected into the experimental animal. In the field of new drug development and basic research, experimental animals are used for a variety of purposes, and according to the variety of research objectives and targets, various types of correction mechanisms to be applied to experiments of experimental animal models are also being proposed.

Among the above experimental animals, experimental rats are used in a relatively large number. In this case, intravenous injection is essentially used, and usually, the rat is held with one hand and the tail is injected with the other hand.

According to the disclosure of Korean Utility Model Registration No. 20-0329108, the experimental rats have thin and weak blood vessels and require technical skill due to the fluctuation of the tail and it is difficult for one person to inject. So, in the past, an auxiliary device was devised to fix the body of an experimental rat inside the barrel, but there was a limitation in use by placing the body inside the barrel so that only the tail vein was secured rather than conveniently and stably fixing the laboratory rat. .

In addition, when a drug is injected into an artery as well as a vein in the tail, it is difficult to identify and find blood vessels in the artery, making it difficult to obtain precise experimental data.

The present invention has been conceived to solve the above problems, stably and quickly fixing the body of a rat for experimentation, and limiting the movement of the tail by removing only the tail separately, so that precise injection can be performed by intravenous administration or blood collection of small animals It is an object of the present invention to provide a correction device.

The present invention is a mounting plate 100 on which an experimental animal with a tail is placed; A fixing part 200 coupled with the seating plate 100 to restrain the experimental animal placed on the seating plate 100; A position control unit 300 coupled to one side of the mounting plate 100 to support the mounting plate 100; And at least one guide plate 400 coupled to the upper surface of the position control unit 300 and configured to be constrained by the experimenter determining the bending direction of the tail. Includes.

The seating plate 100 of the present invention may be coupled to be rotatable about a rotation shaft 110 coupled to the position control unit 300.

The seating plate 100 of the present invention further includes a rotation lever 120 provided with a tail insertion hole 121 through which the tail of the experimental animal is inserted and fixed, and the tail is rotated by the rotation of the rotation lever 120. It can be rotated a predetermined angle.

The position control unit 300 of the present invention may be hinge-coupled with a support arm 600 fixed to one side of the position control unit 300 so as to rotate by a predetermined angle with respect to the ground.

The guide plate 400 of the present invention includes a first guide plate 410 fixed to an upper surface of one side of the position control unit 300 and a first guide groove 440 in which the tail is disposed. A second guide plate 420 may be spaced apart from the plate 410 and fixed to the upper surface of the other side of the position control unit 300.

The first or second guide plate 410, in order to provide a second guide groove 450 for changing the direction of the tail, at the lower side of the first or

second guide plate

410, 420 of the present invention. It may include a third guide plate 430 that is spaced apart from the 420 by a predetermined distance and fixed to the upper surface of the other side of the position control unit 300.

The fixing part 200 of the present invention includes a jacket 210 coated on the body of the experimental animal and a boa system 220 for intimate contact with the seating plate 100, or the fixing part 200 is coupled to the mounting plate 100, may be a mold 230 for pressing the body of the experimental animal.

The present invention constrains the movement of the body of the test subject and minimizes the movement of the tail, so that when the drug is injected into the tail, the injection can be accurately performed, and the angle and height formed by the test subject and the tail can be adjusted. .

In addition, the present invention can easily find and inject a vein or arterial blood vessel in the tail portion due to the configuration of the specimen seating plate and the guide plate that can rotate at a certain angle.

1 is a conceptual diagram illustrating a coarse vein blood collection system including an experimental animal correction device according to the present invention.

Figure 2 is a conceptual diagram showing an embodiment related to the guide plate of the experimental animal correction apparatus according to the present invention.

3 is a conceptual diagram showing another embodiment related to a guide plate among the experimental animal correction apparatus according to the present invention.

Figure 4 is a plan view showing another embodiment related to the guide plate of the experimental animal correction apparatus according to the present invention.

Figure 5 is a conceptual diagram related to the rotation lever of the experimental animal correction device according to the present invention.

The present invention will be described in detail with reference to the accompanying drawings.

1 is an overall conceptual diagram of a coarse vein blood collection or administration system. An experimental animal correction apparatus according to the present invention is shown in order to collect a blood sample from an experimental animal fixed to the experimental animal correction apparatus according to the present invention, in particular, of a rat. The near-infrared rays are irradiated on the upper side of the caudal vein of the experimental animal fixed to the experimental animal correction device, and the position on the caudal vein is accurately determined using an optical microscope. Magnification using a digital camera connected to an optical microscope, and the experimenter can visually check it through a digital viewer.

Thermal action and angiography are possible using a near-infrared irradiation device, and using an optical microscope to magnify the caudal vein of the mouse 10 to 100 times and check the position of the needle with a digital viewer (LCD screen), Administration or blood collection can be performed.

Hereinafter, the experimental animal correction apparatus will be described in detail. This will be described in detail with reference to FIGS. 1 to 3.

The present invention includes a seating plate 100, a fixing part 200, a position control part 300, a guide plate 400, a heating part 500, and the like. A support arm 600 and a base 700 for supporting and fixing them may be further included.

Referring to Figure 1, the base 700 is used for support by being placed on the ground or an experimental table. A support arm 600 for fixing the position control unit 300 or the like, which will be described later, is fixed on the upper part of the base 700. The support arm 600 may be fixed in a state inclined at a predetermined angle with respect to the base 700 in consideration of the user's usability. The slope can be arbitrarily designed in consideration of the experimenter's convenience.

1 to 3, a position control unit 300 and the like are provided above the support arm 600.

[Position control unit (300)]

Referring to FIG. 1, the position control unit 300 serves as a base to which an experimental animal placed on a mounting plate 100 to be described later is fixed. The position control unit 300 may be hinged (not shown) with the support arm 600 described above to be tilted at a predetermined angle. That is, while adjusting the inclination of the position control unit 300 according to the propensity of the experimenter, the position of the caudal vein is identified or the experiment is made easy. In relation to the hinge coupling, since various known techniques may be applied, specific details are avoided.

[Guide plate (400)]

Referring to Figures 1 to 4, the guide plate 400 of the present invention will be described in detail.

The guide plate 400 is disposed on the upper surface of the position control unit 300 described above. A plurality of regularly or irregularly holes or protrusions 310 may be arranged on the upper surface of the position control unit 300 as shown. The guide plate 400 may also have a protrusion or a hole 470 at a position corresponding thereto. Alternatively, a magnet may be provided in all or one of them so that the guide plate 400 and the position control unit 300 may be coupled with a magnet or the like. The above-described holes or

protrusions

310 and 470 may facilitate coupling between the guide plate 400 and the position control unit 300 and serve as a guide for the experimenter to determine the position for various purposes such as experiments.

1 to 3, the guide plate 400 includes first to

third guide plates

410, 420, and 430. The first and

second guide grooves

440 and 450 may be naturally formed by being coupled to the upper surface of the position control unit 300 by the guide plate 400 made of at least three or more plates. The tail of the experimental animal is inserted and fixed inside the first and second guide grooves 440 and 450.

In particular, referring to FIG. 3, the first guide plate 410 may be fixedly coupled to one half-divided surface of the position control unit 300. Of the other half-divided surfaces, a second guide plate 420 is attached to the upper divided surface and a third guide plate 430 is fixed to the position control unit 300 on the lower divided surface. The first and second guide grooves 440 and 450 as described above may be formed between them (see FIGS. 1 and 2 ). Alternatively, referring to FIGS. 3 and 4, the second guide groove 450 may be cut so that the ends of the second and

third guide plates

420 and 430 have a'base (a)' shape. The second guide groove 450 is naturally formed by the coupling of the second and

third guide plates

420 and 430. The tail of the experimental animal may be seated and fixed in the second guide groove 450.

Meanwhile, as shown in FIGS. 2 and 4, the experimenter may arbitrarily change the positions of the first to

third guide plates

410, 420, and 430 for convenience of experimentation. In particular, the second and

third guide plates

420 and 430 may be manufactured symmetrically. For this reason, even if the second and

third guide plates

420 and 430 are flipped in place or moved left and right, it is preferable to be fastened to the position control unit 300 in the same shape or to perform the same function.

3 and 4, a pair of guide rollers 460 may be disposed at a portion bent from the first guide groove 440 to the second guide groove 450 so that the tail of the experimental animal can be firmly fixed. . The guide roller 460 may include a core 461 around the shaft 462 and a louver 461 surrounding the core 461. The louver 461 is a part to which the tail of the experimental animal comes into contact, and may be formed of a material that does not slip, for example, rubber.

As shown in Figs. 1 to 4, it is preferable to fix the tail in a curved state of approximately 90 degrees in order to improve the accuracy of experiments such as accurate microvein check and blood collection by utilizing the above-described configuration.

In order to further improve the accuracy of the experiment, it is desirable to increase the temperature of the tail, so it is preferable that the position control unit 300, the

guide plates

400, 410, 420, 430, and the like are heated. To this end, it is preferable that a heating unit 500 is provided between the position control unit 300 and the

guide plates

400, 410, 420, and 430. Alternatively, the heating unit 500 may be provided on the inner side of the position control unit 300. That is, since the heating unit 500 has the purpose of raising the temperature of the tail of the experimental animal, any location for raising the temperature may be used.

[Seat plate (100)]

1 to 3, the seating plate 100 is fixed to one side of the position control unit 300 so as to be rotatable. As shown in FIG. 3, the rotation shaft 110 is fixed to one side of the position control unit 300. The experimental animal is rotated by rotating the mounting plate 100 on which the body of the experimental animal is fixed. According to the rotation of the experimental animal, the position of the caudal vein or the caudal artery can be accurately varied.

Referring to FIG. 5, a rotation lever 120 is provided on one side of the seating plate 100. It is preferable to configure the rotation lever 120 to be able to rotate alone or to rotate integrally with the seating plate 100. It is preferable that the rotation shaft 110 is provided at an approximately central portion of the rotation lever 110 and the tail insertion hole 121 is provided at an upper central side. The tail insertion hole 121 may be formed in a circular or semicircular shape. When only the rotation lever 120 is independently rotated, it is desirable to form a semicircular shape rather than a circular shape. That is, since only the tail can be twisted (rotated) when the rotation lever 120 is independently rotated, the position of the caudal vein or the caudal artery can be controlled.

On the other hand, the position control unit 300 is provided with a stopper unit 310, it is possible to restrict the rotation degree of the rotation lever 120.

[Government (200)]

The fixing part 200 may be composed of a jacket 210 and a bore system 220, or may be composed of a mold 230, as shown in FIGS. 1 and 2.

As shown in FIGS. 1 and 2, after the jacket 210 is coated on the experimental animal, the experimental animal can be constrained to the seating plate 100 by using the boa system 220. Here, the boA system 220 is a fastening type mainly used for shoes, in which the strap is tightened according to the rotation of the fixed dial using a fixed dial and a strap, and a detailed description thereof will be omitted. Alternatively, a string or the like is fastened together with the jacket 210, and a part of the string is constrained by the seating plate 100 so that the experimental animal may be constrained while wearing the jacket 210.

Meanwhile, as shown in FIG. 3, after the experimental animal is placed on the seating plate 100, the mold 230 may be fastened to the seating plate 100 while pressing the back of the experimental animal. The mold 230 may be made of a frame formed of one mold, and it is preferable that the mold 230 and the fixing part 200 are easily fastened and fixed using a magnet or the like.

Due to the detailed description as described above, after placing the experimental animal on the top of the seating plate 100, the tail can be easily fixed separately for collecting blood vessels or injecting drugs into the caudal vein or the caudal artery. In particular, not only can the experimental animal be fixed very quickly and accurately, but also the tail can be easily fixed in a curved state of about 90 degrees, which will improve the repeatability and accuracy of the experimenter's experiment. .

Claims

A mounting plate 100 on which an experimental animal with a tail is placed;

A fixing part 200 coupled with the seating plate 100 to restrain the experimental animal placed on the seating plate 100;

A position control unit 300 coupled to one side of the mounting plate 100 to support the mounting plate 100; And

At least one guide plate 400 coupled to the upper surface of the position control unit 300 and configured to allow an experimenter to determine and restrain the bending direction of the tail;

Small animal microvenous administration or blood collection correction device comprising a.
The method of claim 1,

The seating plate 100 is a small animal coarse vein administration or blood collection correction device, characterized in that the rotational shaft 110 coupled to the position control unit 300 is coupled to be rotatable.
The method of claim 1,

The seating plate 100 further includes a rotation lever 120 provided with a tail insertion hole 121 through which the tail of the experimental animal is inserted and fixed, and the tail rotates by a predetermined angle by rotation of the rotation lever 120 Small animal microvenous administration or blood collection correction device, characterized in that the.
The method of claim 1,

The position control unit 300 is hinged with a support arm 600 fixed to one side of the position control unit 300 so that the position control unit 300 can rotate by a predetermined angle with respect to the ground. Correction device.
The method of claim 1,

The guide plate 400 includes the first guide plate 410 to provide a first guide plate 410 fixed to an upper surface of one side of the position control unit 300 and a first guide groove 440 in which the tail is disposed. ) And a second guide plate (420) that is spaced a predetermined distance from and fixed to the upper surface of the other side of the position control unit (300).
The method of claim 5,

The first or second guide plates 410 and 420 are provided at the lower side of the first or second guide plates 410 and 420 in order to provide a second guide groove 450 for changing the direction of the tail. Small animal coarse vein administration or blood collection correction device, characterized in that it comprises a third guide plate 430 that is spaced a predetermined distance and fixed to the upper surface of the other side of the position control unit (300).
The method of claim 1,

The fixing part 200 includes a jacket 210 coated on the body of the experimental animal and a boa system 220 for intimate contact with the seating plate 100,

The fixing part 200 is coupled to the seating plate 100 and is a small animal microvenous administration or blood collection correction device, characterized in that it is a mold 230 for pressing the body of the experimental animal.